A sex lure for the diamondback moth and a lure core

Abstract

The application provides a sex lure for Chilo suppressalis and a lure core, and relates to the technical field of insect sex lure.The active ingredient of the sex lure comprises the following raw materials in parts by weight: cis-11-hexadecenal 50 parts, cis-13-octadecenal 4-10 parts, cis-9-hexadecenal 4-10 parts and cis-11-hexadecenol 0.4-200 parts.The sex lure has a strong attracting effect on Chilo suppressalis, is safe for human and livestock, has no pollution to the environment, and can reduce the use of pesticides.The sex lure has a low use cost, is convenient to operate, can be applied to the prediction and control of Chilo suppressalis, and has good social economic and ecological benefits.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of insect sex attractant, and particularly relates to a sex attractant for Chilo suppressalis and a lure core. BACKGROUND

[0002] Chilo suppressalis (Walker) belongs to the class of insects, order of Lepidoptera, and family of Pyralidae, and is widely distributed in the temperate zone, subtropical zone and tropical zone, and is a worldwide rice pest. The distribution of Chilo suppressalis is almost all over the rice areas in China, and the harm of Chilo suppressalis to rice mainly causes withered stem, withered spike, white spike and worm-damaged plant, and greatly reduces the yield of rice. The prevention and control of Chilo suppressalis usually uses chemical pesticides, but over time, Chilo suppressalis inevitably has different degrees of resistance to various pesticides. Chilo suppressalis in many rice areas has developed serious resistance to the commonly used pesticides, and with the increase of global temperature, the change of field cultivation system and the development of pesticide resistance, the prevention and control of Chilo suppressalis is facing crisis, the annual pest population is increasing, and the prevention and control cost is increasing, which causes serious losses to the income of farmers and the production of rice. Therefore, it is necessary to actively seek various control methods to comprehensively control the large occurrence of Chilo suppressalis.

[0003] The sex pheromone technology plays an important role in the comprehensive management of Chilo suppressalis pests. The sex pheromone technology can monitor the occurrence period of Chilo suppressalis through trapping technology to guide the application of pesticides at the right time, and can also reduce the mating rate of female and male moths and the pest population density of the next generation of Chilo suppressalis through mass trapping method. At present, the components of Chilo suppressalis sex pheromone lure core at home and abroad are composed of cis-11-hexadecenyl aldehyde (Z11-16:Ald), cis-13-octadecenyl aldehyde (Z13-18:Ald) and cis-9-hexadecenyl aldehyde (Z9-16:Ald). Further research shows that the mass ratio of the three main components of Chilo suppressalis sex pheromone is 48:6:5 (Jiao Xiaoguo, Xuan Weijian, Wang Hongto, etc. Research progress of Chilo suppressalis sex pheromone control. Insect Knowledge. 2003, 40(3): 193-199). However, a large number of field tests and applications show that the trapping effect of the lure core made by this ratio is not ideal, especially when the population density in the field is low, it is difficult to trap male moths. SUMMARY

[0004] Therefore, the purpose of the present application is to provide a sex attractant for Chilo suppressalis and a lure core, which can not only be used for monitoring the occurrence dynamics of Chilo suppressalis population at low pest population density, but also can prevent and control Chilo suppressalis through mass trapping method of sex pheromone lure core, so as to reduce the use of pesticides, improve the safety of agricultural products, and maintain the stability of ecological environment.

[0005] In order to achieve the above-mentioned purpose of the application, the present application provides the following technical solutions:

[0006] The application provides a sex attractant for Chilo partellus, and active ingredients of the sex attractant include the following raw materials in parts by weight: 50 parts of cis-11-hexadecenal, 4-10 parts of cis-13-octadecenal, 4-10 parts of cis-9-hexadecenal and 0.4-200 parts of cis-11-hexadecenol.

[0007] Preferably, the sex attractant further includes an antioxidant and an anti-ultraviolet light stabilizer; the mass ratio of the antioxidant to cis-11-hexadecenal is 0.002-0.02:1; and the mass ratio of the anti-ultraviolet light stabilizer to cis-11-hexadecenal is 0.002-0.02:1.

[0008] Preferably, the antioxidant includes 2,6-di-tert-butyl-4-methylphenol, di-tert-butyl hydroquinone and antioxidant 168.

[0009] Preferably, the anti-ultraviolet light stabilizer includes a hindered amine anti-ultraviolet light stabilizer; the hindered amine anti-ultraviolet light stabilizer includes UV-328, UV-531 and UV-P.

[0010] The application further provides an attractant core for Chilo partellus, which includes the sex attractant, and the content of the sex attractant is 0.05-50 mg per attractant core.

[0011] Preferably, the attractant core further includes a releaser, and the material of the releaser includes natural rubber, synthetic rubber, polyethylene, polyvinyl chloride, resin or viscose material.

[0012] The application provides a sex attractant for Chilo partellus, and active ingredients of the sex attractant include the following raw materials in parts by weight: 50 parts of cis-11-hexadecenal, 4-10 parts of cis-13-octadecenal, 4-10 parts of cis-9-hexadecenal and 0.4-200 parts of cis-11-hexadecenol. In the sex attractant, cis-11-hexadecenal and cis-13-octadecenal are necessary active components and play a role in long-distance attraction, and cis-9-hexadecenal and cis-11-hexadecenol have been proved to have strong activity through field tests, and significantly enhance the landing and wing-flapping behavior of male moths in a short distance.

[0013] The application further provides an attractant core containing the sex attractant, which can greatly improve the trapping effect of the sex attractant for Chilo partellus through a large number of field tests, and has the advantages of large trapping amount and stable attraction effect, solves the problem of poor control effect of the sex attractant for Chilo partellus, is safe for human and livestock, does not pollute the environment, can reduce the use of pesticides, has low use cost, is easy to operate, is applied to the prediction and control of Chilo partellus, and has good social, economic and ecological benefits. DETAILED DESCRIPTION

[0014] The present application provides a sex attractant for Chilo spp., and the active ingredient of the sex attractant comprises the following raw materials by weight: cis-11-hexadecenal 50 parts, cis-13-octadecenal 4-10 parts, cis-9-hexadecenal 4-10 parts, and cis-11-hexadecenol 0.4-200 parts.

[0015] The active ingredient of the sex attractant comprises cis-11-hexadecenal (Z11-16:Ald), which can play a long-distance attracting role. The source of Z11-16:Ald is not particularly limited, and preferably a Z11-16:Ald standard compound with a purity of more than 98%.

[0016] The active ingredient of the sex attractant comprises cis-13-octadecenal (Z13-18:Ald), and the weight of Z13-18:Ald is preferably 4-8 parts, more preferably 4-6 parts, and most preferably 6 parts. The Z13-18:Ald can play a long-distance attracting role. The source of Z13-18:Ald is not particularly limited, and preferably a Z13-18:Ald standard compound with a purity of more than 98%.

[0017] The active ingredient of the sex attractant comprises cis-9-hexadecenal (Z9-16:Ald), and the weight of Z9-16:Ald is preferably 4-8 parts, more preferably 4-6 parts, and most preferably 5 parts. The Z9-16:Ald can play a short-distance attracting role and can also enhance the attracting effect on Chilo spp. males. The source of Z9-16:Ald is not particularly limited, and preferably a Z9-16:Ald standard compound with a purity of more than 98%.

[0018] The active ingredient of the sex attractant comprises cis-11-hexadecenol (Z11-16:OH), and the weight of Z11-16:OH is preferably 2-100 parts, more preferably 2-80 parts, and most preferably 50 parts. The Z11-16:OH can significantly enhance the attracting effect on Chilo spp. males. The source of Z11-16:OH is not particularly limited, and preferably a Z11-16:OH standard compound with a purity of more than 98%.

[0019] The sex attractant of the present application preferably further comprises an antioxidant and an ultraviolet light stabilizer; the mass ratio of the antioxidant to cis-11-hexadecenal is preferably 0.002-0.02:1, more preferably 0.008-0.02:1, and most preferably 0.01:1. The antioxidant of the present application preferably comprises a mixture of one or more of 2,6-di-tert-butyl-4-methylphenol (BHT), di-tert-butylhydroquinone (DTBHQ) and antioxidant 168. The antioxidant of the present application can prevent oxidation of the aldehyde component in the sex pheromone component. The source of the antioxidant of the present application is not particularly limited and conventional reagents in the art can be used.

[0020] The mass ratio of the ultraviolet light stabilizer to cis-11-hexadecenal is preferably 0.002-0.02:1, more preferably 0.008-0.02:1, and most preferably 0.01:1. The ultraviolet light stabilizer of the present application preferably comprises a hindered amine ultraviolet light stabilizer; the hindered amine ultraviolet light stabilizer preferably comprises UV-328, UV-531 and UV-P. The ultraviolet light stabilizer of the present application can keep the aldehyde component in the sex pheromone component relatively stable. The source of the ultraviolet light stabilizer of the present application is not particularly limited and conventional reagents in the art can be used.

[0021] The present application also provides a sex attractant core for attracting the diamondback moth, comprising the sex attractant, and the content of the sex attractant is 0.05-50 mg per core. The core of the present application preferably further comprises a releasing device, and the material of the releasing device preferably comprises natural rubber, synthetic rubber, polyethylene, polyvinyl chloride, resin or viscose material. The releasing device of the present application can slowly release the sex attractant.

[0022] The raw materials of the sex attractant are respectively configured into heavy n-hexane solutions, mixed in proportion, and then added to the releasing device (such as a silicone rubber plug) after the solvent is naturally volatilized. The sex attractant and the core for attracting the diamondback moth provided by the present application are described in detail below in combination with examples, but they should not be understood as limiting the scope of protection of the present application.

[0023] Example 1

[0024] Test site: Guangchen Town, Jiaxing City, Zhejiang Province.

[0025] Reagents: cis-11-hexadecenal (Z11-16:Ald) standard compound, cis-13-octadecenal (Z13-18:Ald) standard compound, cis-9-hexadecenal (Z9-16:Ald), cis-11-hexadecenol (Z11-16:OH) standard compound, all with a purity of more than 98%.

[0026] Lure preparation: cis-11-hexadecenyl aldehyde, cis-13-octadecenyl aldehyde, cis-9-hexadecenyl aldehyde, cis-11-hexadecenyl alcohol were respectively configured into a certain concentration of heavy distilled n-hexane solution. After mixing according to the set mass ratio, it was added to a silicone rubber plug to obtain the lure.

[0027] Formulation: cis-11-hexadecenyl aldehyde (Z11-16:Ald) 410 μg and cis-13-octadecenyl aldehyde (Z13-18:Ald) 50 μg and cis-9-hexadecenyl aldehyde (Z9-16:Ald) 40 μg and cis-11-hexadecenyl alcohol (Z11-16:OH) 25 μg; anti-ultraviolet light stabilizer UV-328 4.1 μg, antioxidant BHT 4.1 μg.

[0028] Comparative Example 1

[0029] Formulation: cis-11-hexadecenyl aldehyde (Z11-16:Ald) 410 μg and cis-13-octadecenyl aldehyde (Z13-18:Ald) 50 μg and cis-9-hexadecenyl aldehyde (Z9-16:Ald) 40 μg.

[0030] The lures prepared in Example 1 and Comparative Example 1 were tested:

[0031] The test was carried out from May 3 to 21, 2019. Five replicates were set for each treatment, and a boat-shaped trap was selected, and one lure was placed in each trap. The traps were placed in the wheat field, 1.5 m above the ground, one trap every 15 m, and different treatments were placed alternately, and the sticky board was replaced every 5 days, and the number of Chilo suppressalis was counted every 2 days. After each investigation, the positions of the traps were exchanged to reduce the errors caused by the differences in the number of Chilo suppressalis in a small area.

[0032] The effect of the lure on attracting Chilo suppressalis in the field was counted, as shown in Table 1,

[0033] Table 1 Comparison of the effect of different sex pheromone lure formulations on attracting Chilo suppressalis in the field

[0034]

[0035] As can be seen from Table 1, the average number of moths attracted by Example 1 was 72.89% higher than that of Comparative Example 1.

[0036] Example 2

[0037] Test site: Guangchen Town, Jiaxing City, Zhejiang Province.

[0038] Lure preparation: cis-11-hexadecenyl aldehyde, cis-13-octadecenyl aldehyde, cis-9-hexadecenyl aldehyde, cis-11-hexadecenyl alcohol were respectively configured into a certain concentration of heavy distilled n-hexane solution. After mixing according to the set mass ratio, it was added to a silicone rubber plug to obtain the lure.

[0039] Formulation: cis-11-hexadecenyl aldehyde (Z11-16:Ald) 250 μg and cis-13-octadecenyl aldehyde (Z13-18:Ald) 30 μg and cis-9-hexadecenyl aldehyde (Z9-16:Ald) 25 μg with cis-11-hexadecenyl alcohol (Z11-16:OH) 500 μg; Anti-UV light stabilizer UV-531 2.5 μg, antioxidant BHT 2.5 μg.

[0040] Comparative Example 2

[0041] Formulation: cis-11-hexadecenyl aldehyde (Z11-16:Ald) 250 μg and cis-13-octadecenyl aldehyde (Z13-18:Ald) 30 μg and cis-9-hexadecenyl aldehyde (Z9-16:Ald) 25 μg.

[0042] The lures prepared in Example 2 and Comparative Example 2 were tested:

[0043] The test was carried out from April 17 to May 19, 2019. Four replicates were set for each treatment, and new type moth traps were selected, with one lure placed in each trap. The traps were placed in wheat fields, 1.5 m above the ground, with one trap every 15 m, and different treatments were placed alternately. The number of Chilo suppressalis was counted every 7 days or so. After each investigation, the positions of the traps were exchanged to reduce errors caused by differences in the number of Chilo suppressalis in a small area.

[0044] The effect of the lure on attracting Chilo suppressalis in the field was counted, as shown in Table 2,

[0045] Table 2 Comparison of the effect of different sex pheromone lure formulations on attracting Chilo suppressalis in the field

[0046]

[0047] As can be seen from Table 2, the average number of moths attracted by Example 2 was 101.04% higher than that of Comparative Example 2.

[0048] Example 3

[0049] Test site: Guangchen Town, Jiaxing City, Zhejiang Province.

[0050] Lure preparation: cis-11-hexadecenyl aldehyde, cis-13-octadecenyl aldehyde, cis-9-hexadecenyl aldehyde, and cis-11-hexadecenyl alcohol were respectively configured into a certain concentration of heavy n-hexane solution. After mixing according to the set mass ratio, it was added to a silicone rubber plug to obtain the lure.

[0051] Formulation: cis-11-hexadecenyl aldehyde (Z11-16:Ald) 250 μg and cis-13-octadecenyl aldehyde (Z13-18:Ald) 30 μg and cis-9-hexadecenyl aldehyde (Z9-16:Ald) 25 μg and cis-11-hexadecenyl alcohol (Z11-16:OH) 1000 μg; Anti-UV light stabilizer UV-531 2.5 μg, antioxidant BHT 2.5 μg.

[0052] Comparative Example 3

[0053] Formulation: cis-11-hexadecenyl aldehyde (Z11-16:Ald) 250 μg and cis-13-octadecenyl aldehyde (Z13-18:Ald) 30 μg and cis-9-hexadecenyl aldehyde (Z9-16:Ald) 25 μg.

[0054] The lures prepared in Example 3 and Comparative Example 3 were tested:

[0055] The test was carried out from April 17 to May 19, 2019. Four replicates were set for each treatment, and new moth traps were selected, with one lure placed in each trap. The traps were placed in the wheat field, 1.5 m above the ground, with one trap every 15 m, and different treatments were placed alternately. The number of Chilo suppressalis was counted every 7 days or so. After each investigation, the positions of the traps were exchanged to reduce errors caused by differences in the number of Chilo suppressalis in a small area.

[0056] The effect of the lure on attracting Chilo suppressalis in the field was counted, as shown in Table 3,

[0057] Table 3 Comparison of the effect of different sex pheromone lure formulations on attracting Chilo suppressalis in the field

[0058]

[0059] As can be seen from Table 3, the average number of moths attracted by Example 3 was 96.01% higher than that of Comparative Example 3.

[0060] The present application provides a sex lure and lure for attracting Chilo suppressalis, which can significantly enhance the attracting effect of Chilo suppressalis, is safe for humans and animals, does not pollute the environment, and can reduce the use of pesticides. The use cost is lower, the operation is convenient, it can be applied to the prediction and control of Chilo suppressalis, and has good social, economic and ecological benefits.

[0061] The above only describes the preferred embodiments of the present application, and it should be pointed out that those skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A sex attractant for Chilo partellus, characterized by comprising, The active ingredients of the sex attractant include the following raw materials by weight: cis-11-hexadecenal 50 parts, cis-13-octadecenal 6 parts, cis-9-hexadecenal 5 parts and cis-11-hexadecenol 100 parts; The sex attractant further includes an antioxidant and an anti-ultraviolet light stabilizer; the mass ratio of the antioxidant to cis-11-hexadecenal is 0.002-0.02:1; the mass ratio of the anti-ultraviolet light stabilizer to cis-11-hexadecenal is 0.002-0.02:1; The antioxidant includes 2,6-di-tert-butyl-4-methylphenol, di-tert-butyl hydroquinone and antioxidant 168; The anti-ultraviolet light stabilizer includes a hindered amine anti-ultraviolet light stabilizer; the hindered amine anti-ultraviolet light stabilizer includes UV-328, UV-531 and UV-P.

2. A lures core for attracting Chilo uatellus, characterized by, The sex attractant includes the sex attractant of claim 1, and the content of the sex attractant is 0.05-50 mg per lure core.

3. The lure of claim 2, wherein, The lure core further includes a release device, and the material of the release device includes natural rubber, synthetic rubber, polyethylene, polyvinyl chloride, resin or viscose material.

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