A special device and working method for zebrafish larvae tumor transplantation

Abstract

The present invention relates to a special device and working method for zebrafish fry tumor transplantation. The device includes a box body and a transplant body, both of which are rectangular concave bodies with baffles arranged on all sides. When the transplant body is inserted into the box body, the two are combined into a whole. Four rectangular columns are respectively arranged at the four corners of the lower bottom surface of the transplant body, and a plurality of grooves are arranged on the inner bottom surface. A plurality of drainage holes and a ruler are arranged on the inner bottom surface of each groove, and a plurality of groups of scale lines are arranged on the left and right inner walls. The device is not only conducive to fixing the position of the fry during tumor transplantation, which is convenient for operation, but also can accurately quantify the dose of transplanted tumor cells. In addition, the device can also effectively avoid the fry from being accidentally sucked into the fry during the process of absorbing excess liquid before tumor transplantation, causing damage to the fry; it is also conducive to saving transplantation time and avoiding the death of the fry due to lack of water due to excessive operation time. The culture device is ingeniously designed, easy to use, obvious in function, and has good effects, which ensures the accuracy of the experiment and improves the work efficiency.

Description

Technical Field

[0001] The invention relates to a special device and a working method for zebrafish fry tumor transplantation, belonging to the technical field of experimental animal research devices. Background Art

[0002] Model organism tumor transplantation is an important method in tumor biology research. Tumor transplantation is particularly important for analyzing and studying cell malignancy and migration ability, and is the key to identifying potential tumor stem cells. In addition, model organism tumor transplantation also plays an important role in drug research and can be used to screen drugs with tumor-suppressing activity or optimize tumor treatment plans.

[0003] Zebrafish (Danio rerio) is one of the three most commonly used model organisms in the field of life sciences. It has the characteristics of high egg production, low breeding cost, short reproduction cycle, in vitro fertilization, in vitro development, transparent embryos, etc.; it also has physiological and biochemical characteristics similar to mammals. Compared with traditional model organisms such as mice, zebrafish has unique advantages in tumor transplantation: 1) Good tumorigenicity: zebrafish have no adaptive immunity within about 4 to 6 weeks after fertilization. Therefore, human tumor cells have good tumorigenicity after xenotransplantation. Existing data show that about 80% of human tumor cells can survive after transplantation into zebrafish; 2) Intuitive and diverse indicators: zebrafish fry are transparent and easy to observe; in addition to evaluating tumor tissue size, survival rate and survival time, it is also possible to observe abnormal vascular proliferation induced by tumor cells, the relationship between tumor metastasis and vascular proliferation, etc.; 3) Short experimental cycle: pharmacodynamic evaluation can be completed within 3 days after administration. A large number of verification experiment results show that the anti-cancer pharmacodynamic evaluation results obtained by administering drugs to zebrafish for 3 days are very predictive; 4) Small sample amount: Early sample preparation was very difficult, and the zebrafish model only required about 10 mg of sample to complete the experiment, saving the time and cost of sample preparation; 5) Conservative biological characteristics: A large number of literatures show that after human tumor cells are transplanted into zebrafish, their biological characteristics are basically stable.

[0004] When zebrafish are transplanted with tumors, the fry are usually placed in a culture dish, and then combined with microinjection technology, the dose of the transplanted tumor is controlled by adjusting the injection pressure and injection time. However, since the length and thickness of the injection needle seriously affect the injection volume, if the injection needle is replaced under the same injection pressure and injection time, the injection volume is likely to change, and the corresponding dose of the transplanted tumor will also change. When the injection pressure and time are constant, it is impossible to accurately quantify the injection volume by observing the volume of the injection solution in the fry with the naked eye and the help of an indicator, and thus it is impossible to accurately calculate the actual dose of the transplanted tumor.

[0005] In view of the defects of existing culture dishes for tumor transplantation, it is urgent to design a special device for zebrafish larvae tumor transplantation to solve the problem of inaccurate exogenous tumor transplantation dosage in the existing transplantation process. Summary of the invention

[0006] In view of the deficiencies of the prior art, the present invention provides a special device for zebrafish fry tumor transplantation.

[0007] The invention also provides a working method of the device.

[0008] The technical solution of the present invention is as follows:

[0009] A special device for zebrafish larvae tumor transplantation, comprising a box body and a transplant body;

[0010] The box body and the transplant body are both rectangular concave bodies with baffles arranged around them. The transplant body is located in the concave body of the box body. The inner bottom surface of the transplant body is provided with at least two rectangular grooves. The bottom of each rectangular groove is provided with at least two circular drainage holes and a ruler. At least two groups of scale lines are provided on the inner longitudinal wall of each rectangular groove.

[0011] Four rectangular columns are arranged at the four corners of the outer bottom surface of the transplant body.

[0012] After the transplant is inserted into the box body, it is combined into a whole. The advantage of this design is that the rectangular box body and the transplant are easy to process and manufacture, and the rectangular transplant body has a large internal space, which is convenient for setting multiple grooves; the rectangular groove inside the transplant body is similar to the body shape of the zebrafish fry, which is convenient for fixing the fish body during tumor transplantation; during tumor transplantation, a pipette is used to put the fry into the groove of the transplant body, and the excess liquid in the pipette can enter the box body through the drainage hole at the bottom of the groove, thereby avoiding interference with the transplantation operation. At the same time, the bottom of the box body remains dry and can directly enter the next observation procedure. In addition, when there is too much liquid in the box body, in order to prevent the liquid from overflowing into the transplant body again, the transplant body can be taken out of the box body, so as to pour out the liquid in the box body, and the transplant body taken out can be fixed on the operating table through four rectangular columns, and its bottom surface can be kept from contacting the operating table, thereby avoiding contamination.

[0013] Preferably, the baffles on the two sides of the transplant body are arranged opposite to each other in an inclined manner. The advantage of this design is that the baffles on the two sides are arranged as slopes, which makes it convenient to take and put the transplant body in the box.

[0014] Preferably, the two opposite sides of the transplant body are provided with horizontal handles, and the horizontal handles are connected to the upper edge of the baffle of the transplant body. The advantage of this design is that the horizontal handles are provided on the left and right sides of the transplant body, which is convenient for taking the transplant body from the box body.

[0015] Further preferably, two arc-shaped grooves are arranged on the upper surfaces of the two oppositely arranged horizontal handles of the transplant body. The advantage of this design is that two arc-shaped grooves are arranged on the left and right horizontal handles of the transplant body respectively, which facilitates the temporary fixation of the needle holder during tumor transplantation.

[0016] Preferably, the ruler is arranged in the middle of the rectangular groove. The advantage of this design is that when multiple zebrafish fry are transplanted with tumors, the zebrafish fry are arranged in sequence on the left and right sides of the ruler, which can effectively prevent the fry from moving during the tumor transplantation process and facilitate the transplantation. The ruler can also be used to measure the diameter of the spherical droplets formed after the culture medium containing tumor cells enters the fry, and then the precise dose of tumor cells transplanted each time can be calculated.

[0017] Preferably, the number of the scale lines is 4-10 groups, and the distance between each group of scale lines is 2-7 mm.

[0018] Further preferably, the number of the scale lines is 6 groups, and the distance between each group of scale lines is 4 mm.

[0019] Further preferably, each group of scale lines includes 4 scales, and the corresponding scales are 1 mm, 2 mm, 3 mm, and 4 mm, respectively. The advantage of this design is that when zebrafish fry are undergoing tumor transplantation, the scale lines can be used to assist in measuring the diameter of the spherical droplets formed after the culture medium containing tumor cells enters the fry, thereby accurately calculating the dose of tumor cells transplanted each time.

[0020] Preferably, the rectangular column is hinged to the outer bottom surface of the transplant body, and the four rectangular columns are connected by three connecting rods, and one of the rectangular columns is also hinged with an elbow hanging rod. The rectangular column can be rotated to be parallel to the outer bottom surface of the transplant body, which can further shorten the space between the transplant body and the box body, and avoid the overall height of the device affecting subsequent microscopic observation. When it needs to be erected, the elbow hanging rod is pulled, and the four columns are brought to an upright state under the action of the connecting rod, and then the elbow hanging rod is hung on the edge of the transplant body. When it needs to be folded, the elbow hanging rod is removed to rotate the rectangular column to a horizontal state, and then it can be folded.

[0021] Preferably, a filter screen is provided at the bottom of the rectangular groove, and a scale is provided horizontally above the filter screen. The filter screen is used to replace the circular drainage hole to filter out excess water.

[0022] Preferably, the number of the rectangular grooves is 3-6.

[0023] Further preferably, the number of the rectangular grooves is 5.

[0024] Preferably, the number of the circular drainage holes is 20-60.

[0025] Further preferably, the number of the circular drainage holes is 40.

[0026] Preferably, the transparent box body is 70-110 mm long, 50-70 mm wide, and 1.5-2.5 mm thick.

[0027] Further preferably, the box body is 90 mm long, 60 mm wide and 2 mm thick.

[0028] Preferably, the transplant body is 50-100 mm long, 45-60 mm wide, and 2-6 mm thick.

[0029] More preferably, the transplant body is 80 mm long, 55 mm wide, and 5 mm thick.

[0030] Preferably, the baffle of the box body is 10-30 mm high and 1.5-2.5 mm thick.

[0031] Further preferably, the baffle of the box body is 25 mm high and 2 mm thick.

[0032] Preferably, the baffle of the transplant body is 10-30 mm high and 0.5-2.5 mm thick.

[0033] Further preferably, the baffle of the transplant body is 20 mm high and 1 mm thick.

[0034] Preferably, the horizontal handle is 45-60 mm long, 5-20 mm wide, and 5-13 mm thick.

[0035] Further preferably, the horizontal handle is 55 mm long, 15 mm wide, and 9 mm thick.

[0036] Preferably, the arc-shaped groove is 45-60 mm long, 4-8 mm deep, and the wide mouth is 6 mm-15 mm long.

[0037] Further preferably, the arc-shaped groove is 55 mm long, 7 mm deep, and the wide mouth is 11 mm long.

[0038] Preferably, the rectangular column is 1-4 mm long, 1-4 mm wide, and 2-5 mm high.

[0039] Further preferably, the rectangular column is 3 mm long, 2 mm wide and 4 mm high.

[0040] Preferably, the rectangular groove is 40-50 mm long, 5-15 mm wide, and 1.5-4.7 mm deep; and the distance between adjacent rectangular grooves is 3-7 mm.

[0041] Further preferably, the rectangular groove is 45 mm long, 10 mm wide, and 4.5 mm deep; and the distance between adjacent rectangular grooves is 5 mm.

[0042] Preferably, the ruler is 40-50 mm long, 1-3 mm wide, and 0.1-0.7 mm thick.

[0043] Further preferably, the ruler is 45 mm long, 2 mm wide, and 0.3 mm thick.

[0044] Preferably, the diameter of the circular drainage hole is 0.5-2 mm.

[0045] Further preferably, the diameter of the circular drainage hole is 1 mm.

[0046] Preferably, the minimum unit of the scale mark is 0.05-3 mm.

[0047] Further preferably, the minimum unit of the scale mark is 0.1 mm.

[0048] A working method using the above-mentioned dedicated device for zebrafish fry tumor transplantation comprises the following steps:

[0049] Insert the transplant into the box, use a pipette to transfer the zebrafish fry into the groove of the transplant, move the transplant out of the box, pour out the liquid in the box, reinsert the transplant into the box, use a needle holder to perform tumor transplantation, and measure the diameter of the spherical droplet formed after the culture medium containing tumor cells enters the fry.

[0050] The beneficial effects of the present invention are:

[0051] The present invention provides a special device for zebrafish fry tumor transplantation, which is provided with a transplant body, in which a plurality of rectangular grooves are provided, and the inner bottom surface and inner side wall of the rectangular groove are respectively provided with a ruler and a scale line. When the tumor is transplanted, the fry is placed on both sides of the ruler in the rectangular groove, which is conducive to adjusting the position of the fry so that they are arranged neatly, preventing the position of the fry from moving during the transplantation process, and facilitating tumor transplantation; and the diameter of the spherical droplet formed after the culture fluid containing tumor cells enters the fry can be measured with the help of the ruler and the scale line, and then according to the spherical volume V=πd 3 / 6, calculate the exact dose of tumor cells transplanted each time.

[0052] The technical solution of the present invention is provided with horizontal handles on the left and right sides of the transplant body, and the horizontal handles are provided with arc-shaped grooves. During the experiment, if the tumor transplantation is temporarily stopped, the needle holder can be temporarily fixed in the arc-shaped groove to prevent the needle holder from rolling and avoid the injection needle from breaking due to the rolling of the needle holder.

[0053] The present invention arranges four rectangular columns at the four corners of the lower bottom surface of the transplant body, and arranges a plurality of rectangular grooves on the inner bottom surface, and circular drainage holes are arranged in the rectangular grooves. When the tumor is transplanted, the transplant body is inserted into the box body and combined into a whole; a pipette is used to put the young fish into the groove of the transplant body, and the excess water in the groove can be quickly discharged into the box body through the drainage holes at the bottom of the groove, which can avoid the young fish from being accidentally sucked when absorbing water, thereby avoiding the harm to the young fish; it is also beneficial to save experimental time, avoid the death of the young fish due to long-term lack of water, and because the lower surface of the box body is dry, it can avoid residual droplets from contaminating the microscope, and facilitate experimental operation. In addition, when there is too much water in the box body, the transplant body can be taken out of the box body, and the water in the box body can be poured out, thereby avoiding the water from overflowing into the transplant body again and affecting the experimental operation. The transplant body taken out can be fixed on the operating table through the four rectangular columns, and its lower bottom surface cannot contact the operating table, thereby avoiding contamination. It is easy to use, has obvious effects, and has good effects, ensuring the accuracy of the experiment and improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] Figure 1 It is a schematic diagram of the structure of the box body in the present invention.

[0055] Figure 2 It is a schematic diagram of the structure of the transplant body in the present invention.

[0056] Figure 3 It is a top view of the box body in the present invention.

[0057] Figure 4 It is a top view of the transplant body of the present invention.

[0058] Figure 5 It is a schematic diagram of the assembly of the box body and the transplant body in the present invention.

[0059] Figure 6 It is a schematic diagram of the structure of the transplant body and the rectangular column in the present invention.

[0060] Wherein: 1. box body, 101. box body baffle, 102. box body baffle, 2. transplant body, 201. transplant body baffle, 202. transplant body baffle, 3. rectangular column, 4. rectangular groove, 401. drainage hole, 402. ruler, 403. scale line, 5. horizontal handle, 501. arc-shaped groove. DETAILED DESCRIPTION

[0061] The invention will be further described below by way of embodiments in conjunction with the accompanying drawings, but is not limited thereto.

[0062] Example 1

[0063] A special device for zebrafish larvae tumor transplantation, such as Figure 1-5As shown, it includes a transparent box body 1 and a transplant body 2, and the box body 1 and the transplant body 2 are combined into a whole. The material of the box body 1 is transparent glass or plastic sheet; the shape of the box body 1 is rectangular. Baffles 101 and 102 are arranged on the four sides of the rectangular box body 1, and the materials of the baffles 101 and 102 are glass or plastic. The entire box body 1 is surrounded by the baffles on all sides to form a rectangular concave body. The material of the transplant body 2 is transparent glass or plastic sheet; the shape of the transplant body 2 is rectangular, and baffles 201 and 202 are arranged on the four sides of the rectangular transplant body 2, and the materials of the baffles 201 and 202 are glass or plastic. The entire transplant body 2 is surrounded by the baffles on all sides to form a rectangular concave body.

[0064] The inner bottom surface of the transplant body is provided with five rectangular grooves 4, each of which is provided with 40 circular drainage holes 401 and a ruler 402 at the bottom. The ruler is provided in the middle of the rectangular groove, and six groups of scale lines 403 are provided on the inner longitudinal wall of each rectangular groove.

[0065] Four rectangular columns 3 are arranged at the four corners of the outer bottom surface of the transplant body. Among them, the baffles 202 on the left and right sides of the transplant body 2 are arranged at an angle, and horizontal handles 5 are arranged at the relative positions on the left and right sides of the transplant body 2. The arrangement of the inclined baffles 202 and the horizontal handles 5 facilitates the removal and placement of the transplant body 2 from the box body 1. Two arc-shaped grooves 501 are respectively arranged on the upper surfaces of the horizontal handles 5 on the left and right sides. The arrangement of the arc-shaped grooves 501 facilitates the temporary fixation of the needle holder during the tumor transplantation operation.

[0066] When performing tumor transplantation, the transplant body 2 is inserted into the box body 1, and the zebrafish fry is transferred to the groove 4 of the transplant body 2 by using a pipette. The excess water in the groove 4 can be quickly drained into the box body 1 through the drainage hole 401 at the bottom of the groove 4, which can avoid the fry from being accidentally sucked when absorbing water, thereby avoiding damage to the fry; it is also beneficial to save the operation time of tumor transplantation, avoid the death of zebrafish fry due to lack of water due to too long operation time, and because the lower surface of the box body 1 is dry, it can avoid residual droplets from contaminating the microscope, which is convenient for experimental operation. When there is too much water in the box body 1, the transplant body 2 can be taken out of the box body 1, and the water in the box body 1 can be poured out, so as to avoid the water from overflowing into the transplant body again and affecting the experimental operation. The transplant body 2 taken out can be fixed on the operating table through four rectangular columns 3, and its lower bottom surface cannot contact the operating table, thereby avoiding contamination.

[0067] When performing tumor transplantation, the zebrafish fry are placed on both sides of the ruler 402 in the groove 4 to prevent the fry from moving during the transplantation process, making the transplantation operation easier. The ruler 402 and the scale line 403 in the groove 4 can also be used to measure the diameter of the spherical droplets formed after the culture fluid containing tumor cells enters the fry, thereby calculating the precise dose of tumor cells transplanted each time.

[0068] Example 2

[0069] A special device for zebrafish fry tumor transplantation, the structure of which is as described in Example 1, except that it includes a transparent box body and a transplant body, and the box body and the transplant body are combined into a whole. The box body is 70mm long, 50mm wide, and 1.5mm thick, and the surrounding baffles are 10mm high and 1.5mm thick. The transplant body is 50mm long, 45mm wide, and 2mm thick, and the surrounding baffles are 10mm high and 0.5mm thick. The horizontal handles on both sides are 45mm long, 5mm wide, and 5mm thick. The horizontal handles are provided with arc-shaped grooves with a length of 45mm, a depth of 4mm, and a wide mouth length of 6mm.

[0070] Four rectangular columns with a length of 2mm, a width of 1mm, and a height of 2mm are set at the four corners of the lower bottom of the transplant body; four rectangular grooves with a spacing of 7mm, a length of 40mm, a width of 5mm, and a depth of 1.5mm are set on the inner bottom surface. A ruler and 30 circular drainage holes with a diameter of 2mm are set on the inner bottom surface of each rectangular groove; four groups of scale lines with a spacing of 7mm are set on the left and right inner walls, and each group of scale lines has a total of 4 lines, corresponding to 1mm, 2mm, 3mm, and 4mm respectively. The ruler in the rectangular groove is 40mm long, 1mm wide, and 0.2mm thick, and the smallest unit is 0.05mm.

[0071] Example 3

[0072] A special device for zebrafish fry tumor transplantation, the structure of which is as described in Example 1, except that the box body is 110 mm long, 70 mm wide, and 2.5 mm thick, and the surrounding baffles are 30 mm high and 2.5 mm thick. The transplant body is 100 mm long, 60 mm wide, and 6 mm thick, and the surrounding baffles are 30 mm high and 2.5 mm thick. The horizontal handles on both sides are 60 mm long, 20 mm wide, and 13 mm thick. The horizontal handles are provided with arc-shaped grooves with a length of 60 mm, a depth of 8 mm, and a wide mouth length of 15 mm.

[0073] Four rectangular columns with a length of 4mm, a width of 3mm, and a height of 5mm are set at the four corners of the lower bottom of the transplant body, and six rectangular grooves with a spacing of 3mm, a length of 50mm, a width of 15mm, and a depth of 4.7mm are set on the inner bottom surface. A ruler and 60 circular drainage holes with a diameter of 0.5mm are set on the inner bottom surface of each rectangular groove; 8 groups of scale lines with a spacing of 2mm are set on the left and right inner walls, and each group of scale lines has a total of 4 lines, corresponding to 1mm, 2mm, 3mm, and 4mm respectively. The ruler in the rectangular groove is 50mm long, 3mm wide, and 0.7mm thick, and the smallest unit is 3mm.

[0074] Example 4

[0075] A special device for zebrafish fry tumor transplantation, the structure of which is as described in Example 1, except that the box body is 90 mm long, 60 mm wide, and 2 mm thick, and the surrounding baffles are 25 mm high and 2 mm thick. The transplant body is 80 mm long, 55 mm wide, and 5 mm thick, and the surrounding baffles are 20 mm high and 1 mm thick. The horizontal handles on both sides are 55 mm long, 15 mm wide, and 9 mm thick. The horizontal handles are provided with arc-shaped grooves with a length of 55 mm, a depth of 7 mm, and a wide mouth length of 11 mm.

[0076] Four rectangular columns with a length of 3mm, a width of 2mm, and a height of 4mm are set at the four corners of the lower bottom of the transplant body, and five rectangular grooves with a spacing of 5mm, a length of 45mm, a width of 10mm, and a depth of 4.5mm are set on the inner bottom surface. A ruler and 40 circular drainage holes with a diameter of 1mm are set on the inner bottom surface of each rectangular groove; 6 groups of scale lines with a spacing of 4mm are set on the left and right inner walls, and each group of scale lines has a total of 4 lines, corresponding to 1mm, 2mm, 3mm, and 4mm respectively. The ruler in the rectangular groove is 45mm long, 2mm wide, and 0.3mm thick, and the smallest unit is 0.1mm.

[0077] Example 5

[0078] A special device for zebrafish fry tumor transplantation, the structure of which is as described in Example 1, except that the rectangular column is hinged to the outer bottom surface of the transplant body, the four rectangular columns are connected by three connecting rods, and one of the rectangular columns is also hinged with an elbow hanging rod, such as Figure 6 As shown. The rectangular column can be rotated to be parallel to the outer bottom surface of the transplant body, which can further shorten the space between the transplant body and the box body, and prevent the overall height of the device from affecting subsequent microscopic observation. When it needs to be erected, the elbow hanging rod is pulled, and the four columns are made upright under the action of the connecting rod, and then the elbow hanging rod is hung on the edge of the transplant body. When it needs to be folded, the elbow hanging rod is removed to rotate the rectangular column to a horizontal position and then folded.

[0079] Example 6

[0080] A special device for zebrafish fry tumor transplantation, the structure of which is as described in Example 1, except that a filter is provided at the bottom of the rectangular groove, and a scale is provided above the filter. The filter is used to replace the circular drainage hole to filter out excess water.

Claims

1. A special device for zebrafish fry tumor transplantation, characterized in that: comprising a box body and a transplant body; The box body and the transplant body are both rectangular concave bodies with baffles arranged around them. The transplant body is located in the concave body of the box body. The inner bottom surface of the transplant body is provided with at least two rectangular grooves. The bottom of each rectangular groove is provided with at least two circular drainage holes and a ruler. At least two groups of scale lines are provided on the inner longitudinal wall of each rectangular groove. The baffles on the two sides of the transplant body that are arranged opposite to each other are arranged obliquely. The ruler is arranged in the middle of the rectangular groove. The number of scale lines is 4-10 groups, and the distance between each group of scale lines is 2-7mm. Each group of scale lines includes 4 scales, and the corresponding scales are 1mm, 2mm, 3mm, and 4mm respectively. Four rectangular columns are arranged at the four corners of the outer bottom surface of the transplant body; the rectangular columns are hinged to the outer bottom surface of the transplant body, the four rectangular columns are connected by three connecting rods, and one of the rectangular columns is also hinged with an elbow hanging rod.

2. The dedicated device for zebrafish fry tumor transplantation according to claim 1, characterized in that: Horizontal handles are arranged on two opposite sides of the transplant body, and the horizontal handles are connected to the upper edge of the baffle of the transplant body.

3. The dedicated device for zebrafish fry tumor transplantation according to claim 2, characterized in that: Two arc-shaped grooves are arranged on the upper surfaces of the horizontal handles on both sides of the transplant body which are arranged opposite to each other.

4. The dedicated device for zebrafish fry tumor transplantation according to claim 1, characterized in that: The number of the scale lines is 6 groups, and the distance between each group of scale lines is 4 mm.

5. The dedicated device for zebrafish fry tumor transplantation according to claim 3, characterized in that: The number of the rectangular grooves is 3-6, the number of the circular drainage holes is 20-60, the box body is 70-110mm long, 50-70mm wide and 1.5-2.5mm thick, the transplant body is 50-100mm long, 45-60mm wide and 2-6mm thick, the baffle of the box body is 10-30mm high and 1.5-2.5mm thick, the baffle of the transplant body is 10-30mm high and 0.5-2.5mm thick, the horizontal handle is 45-60mm long, 5-20mm wide and 5-13mm thick, The arc-shaped groove is 45-60mm long, 4-8mm deep, and 6mm-15mm long at the wide mouth. The rectangular column is 1-4mm long, 1-4mm wide, and 2-5mm high. The rectangular groove is 40-50mm long, 5-15mm wide, and 1.5-4.7mm deep. The distance between adjacent rectangular grooves is 3-7mm. The ruler is 40-50mm long, 1-3mm wide, and 0.1-0.7mm thick. The diameter of the circular drainage hole is 0.5-2mm, and the minimum unit of the ruler scale is 0.05-3mm.

6. The dedicated device for zebrafish fry tumor transplantation according to claim 5, characterized in that: The number of the rectangular grooves is 5, the number of the circular drainage holes is 40, the box body is 90mm long, 60mm wide and 2mm thick, the transplant body is 80mm long, 55mm wide and 5mm thick, the baffle of the box body is 25mm high and 2mm thick, the baffle of the transplant body is 20mm high and 1mm thick, the horizontal handle is 55mm long, 15mm wide and 9mm thick, the arc-shaped groove is 55mm long, 7mm deep, and the wide mouth is 11mm long, the rectangular column is 3mm long, 2mm wide and 4mm high, the rectangular groove is 45mm long, 10mm wide and 4.5mm deep; the distance between adjacent rectangular grooves is 5mm, the ruler is 45mm long, 2mm wide and 0.3mm thick, the diameter of the circular drainage hole is 1mm, and the minimum unit of the ruler scale is 0.1mm.

7. A method for using the dedicated device for zebrafish fry tumor transplantation according to claim 1, characterized in that: The method comprises the following steps: inserting a transplant into a box body, transferring zebrafish fry into a groove of the transplant using a pipette, moving the transplant out of the box body, pouring out the liquid in the box body, reinserting the transplant into the box body, performing tumor transplantation using a needle holder, and measuring the diameter of a spherical droplet formed after a culture fluid containing tumor cells enters the fry.

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