Preparation method and application of daylily cut flower preservative

Abstract

A method for preparing a daylily cut flower preservative comprises the following steps: (1) preparing garlic juice: (2) mixing, and its application comprises the following steps: 1. cutting time; 2. pre-treatment before vase insertion; 3. preservation treatment. The present invention utilizes organic acids and nutrients contained in natural extract lemon juice, and the pH value of the solution is 3-4, which inhibits bacterial growth and supplements the vitamins and trace elements required for cut flower preservation; utilizes the natural bactericidal components contained in garlic juice to make the preservative itself have disinfection and sterilization functions, replaces the chemical preservatives on the market, and avoids the product unsafe due to the artificial addition of chemical substances; distilled water reduces the chance of various microorganisms and bacteria breeding; the formula composition is simple, the nutrients are comprehensive, the preparation is convenient, the cost is low, and the preservation effect is obvious, which overcomes the problems of expensive cut flower preservation equipment, harmful chemical preservatives to the human body and the environment, and poor preservation effect in the prior art, and has no harmful effects on the human body and is environmentally friendly and pollution-free.

Description

Technical Field

[0001] The invention belongs to the technical field of fresh cut flower preservation, and particularly relates to a preparation method and application of a daylily cut flower preservative. Background Art

[0002] Daylily, also known as daylily and daylily, is a special vegetable that is practical, medicinal and ornamental. In recent years, with the expansion of the cultivated area, the industry development has entered a bottleneck period, and extending the product industry chain has become an inevitable trend for its sustainable development. At present, deep-processing products have entered the stage of diversified research and development and application. Exploring the culture of mother flowers and conducting research on fresh cut flowers has become another development direction. However, during the period of vase insertion, the flowering of daylily cut flowers gradually shrinks, the buds are difficult to grow, and the buds are easy to fall off. When inserted in a bottle with distilled water, the lifespan is only about 3 days.

[0003] At present, there are few reports on the preservation of cut daylily flowers. Although other cut flower preservatives used in the market can also extend the vase life of cut daylily flowers, most of them are chemical preservatives, which lack safety for edible daylily flowers. Therefore, it is urgent to develop a safe and non-toxic fresh cut daylily flower preservative, which can be used to enjoy the flowers and eat the flower buds at ease, and has a good guiding significance for the development direction of the daylily industry. Summary of the invention

[0004] In view of the problems raised in the background technology that the existing preservatives on the market are inedible, non-specialized, and have a short shelf life, the present invention studies and designs a day lily cut flower preservative and a method of using the same, with the purpose of providing a preparation method and application of a day lily cut flower preservative whose flowers can be viewed and buds can be eaten during vase placement and has a long vase life.

[0005] The technical solution of the present invention:

[0006] A method for preparing a daylily cut flower preservative, characterized in that it comprises the following steps: (1) preparing garlic juice: weighing 100g of garlic and putting it into a juicer, adding 200ml of pure water, squeezing the juice for 40s and filtering it with gauze, taking the filtrate as garlic juice for later use; (2) mixing: weighing 30-50g of white sugar with an analytical balance, measuring 5-10ml of lemon juice, 10-15ml of garlic juice, and 5-10ml of a growth regulator with a pipette, dissolving them in 1L of pure water in turn, mixing and stirring evenly, thereby obtaining the daylily cut flower preservative of the present invention.

[0007] The preparation method of the lemon juice in the mixture of step (2) is as follows: wash and dry the lemons, cut them in half, put on disposable gloves, squeeze out the fresh lemon juice, and filter it with a single layer of gauze for later use.

[0008] The preparation method of the growth regulator in the mixture of step (2) is as follows: weigh 0.5 g of the growth promoter, dissolve it in 95% alcohol, dilute it to 50 ml with distilled water, and put it into a reagent bottle for later use.

[0009] Preferably, the mixture in step (2) contains 30 g of white sugar, 5 ml of lemon juice, 10 ml of garlic juice and 5 ml of growth regulator.

[0010] The growth promoter is one of the biochemical reagents IAA, GA3, KT, 2,4-D, NAA, and 6-BA produced by Jinan Plant Biotechnology Co., Ltd., and the purity of each of the biochemical reagents is ≥99%.

[0011] The invention discloses an application of a daylily cut flower preservative, characterized in that the application comprises the following steps: 1. Flower cutting time: after the first flower bud reaches the picking standard, the flower stalk is cut; 2. Pre-treatment before vase insertion: according to the height of the vase, the redundant flower stalk is cut off, and the base of the lowest inflorescence axis is preferably equal to or higher than 10 cm from the vase mouth, and the flower stalk is cut at an angle of 45° with a utility knife whose blade is wiped clean with 75% alcohol; 3. Preservative treatment: the treated flower stalk is inserted into a vase filled with fresh flower preservative liquid, the height of the vase is 25-35 cm, the preservative liquid can be replaced every 3-4 days to extend the vase life, and the flower branches are placed in a place with sufficient light to facilitate flowering. This method can maintain flowering for 7-14 days, and more than 90% of the flower buds meet the edible standard.

[0012] Preferably, the height of the vase in the fresh-keeping treatment in step 3 is 28 cm.

[0013] The invention has the following beneficial effects: the invention utilizes organic acids and nutrients contained in natural extract lemon juice to adjust the pH value of the solution to 3-4, inhibits bacterial growth, and achieves the purpose of supplementing vitamins and trace elements required for cut flower preservation; utilizes natural bactericidal components contained in garlic juice to enable the preservative itself to have disinfection and sterilization functions, replaces chemical preservatives on the market, and avoids product unsafety caused by artificial addition of chemical substances; utilizes distilled water that does not contain harmful ions that have side effects on flowers, and at the same time, reduces the chance of various microorganisms and bacteria growing; the above formula has simple components, comprehensive nutrients, convenient preparation, low cost, and obvious preservation effect, overcomes the problems in the prior art that the cut flower preservation equipment is expensive, or the chemical preservative is harmful to the human body and the environment, and the preservation effect is poor, has no harmful effects on the human body, is friendly to the environment and has no pollution, is easily accepted by ordinary families and the market, and is suitable for various flower business places and offices and families. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 Effects of different plant growth regulators on the bud weight of cut daylily flowers.

[0015] Figure 2Effects of different plant growth regulators on the bud length of cut daylily flowers.

[0016] Figure 3 The effects of different plant growth regulators on the flower length of daylily.

[0017] Figure 4 Effects of different plant growth regulators on the development of cut daylily flowers.

[0018] Figure 5 Effects of different plant growth regulators on the unit weight of cut daylily flowers.

[0019] Figure 6 Effects of different plant growth regulators on the soluble sugar content in cut daylily buds.

[0020] Figure 7 This study is about the effects of different plant growth regulators on the VC content in cut daylily flower buds.

[0021] Figure 8 Effects of different plant growth regulators on the MDA content of cut daylily flowers.

[0022] Fig. 9 Effects of different plant growth regulators on the SOD content of cut daylily flowers.

[0023] Fig.10 Effects of different plant growth regulators on the proline content in cut daylily flowers. DETAILED DESCRIPTION

[0024] The present invention is further described below in conjunction with specific embodiments, but the present invention is not limited to these embodiments.

[0025] The test variety was eggplant flower. The seedlings were purchased from the Yellow Flower Research Institute in Shaodong County, Hunan Province. They were transplanted to the Yunhe Bay Base of Suqian Academy of Agricultural Sciences in August 2016. Starting from July 17, 2019, the flower stalks were collected in three batches every 7 days. The first-level flower branches with strong plants and no diseases and insect pests were selected. The harvesting standard was that the first flower was fully open and the other flowers were in the bud state (12 to 15 effective flower buds). They were picked at 16:00 and transported to the laboratory after harvesting. The flower stalks were trimmed and cut into a 45° bevel with a sterilized single-sided blade so that the lowest branch was about 10 cm higher than the bottle mouth.

[0026] Example 1 (T1)

[0027] The trimmed cut flower vases were inserted into a loose-mouthed vase containing 1L of preservative and placed in a sunny place indoors. The pH value of the preservative was maintained at 3-4. The preservative ingredients and concentrations were 30g·L-1 sucrose, 5ml·L-1 lemon juice, 5g·L-1 garlic juice, and 50mg·L-1 IAA. Each group had 3 bottles (repeated 3 times), and each bottle had 5 flowers for observation of the preservation effect. Each group had 3 bottles of the same repetition for determination of physiological indicators. The preservation treatment was carried out at room temperature, with an average laboratory temperature of 25±2℃ and a relative humidity of 60%-80%.

[0028] Example 2 (T2)

[0029] The trimmed cut flowers were placed in a loose-mouthed vase containing 1L of preservative and placed in a sunny place indoors. The pH value of the preservative was maintained at 3-4. The ingredients and concentrations of the preservative were 30g·L-1 sucrose, 5ml·L-1 lemon juice, 5g·L-1 garlic juice, 50mg·L-1 GA 3 Each group has 3 bottles (3 replicates), 5 flowers per bottle, for observation of preservation effect, and each group has 3 bottles of the same replicates for determination of physiological indicators. The preservation treatment was carried out at room temperature, with an average laboratory temperature of 25±2℃ and a relative humidity of 60% to 80%.

[0030] Example 3 (T3)

[0031] The above-mentioned trimmed cut flower bottles were inserted into a loose-mouthed vase containing 1L of preservative and placed in a sunny place indoors. The pH value of the preservative was maintained at 3-4, and the preservative ingredients and concentrations were 30g·L-1 sucrose, 5ml·L-1 lemon juice, 5g·L-1 garlic juice, 50mg·L-1 KT. There were 3 bottles in each group (repeated 3 times), 5 flowers in each bottle, for observation of the preservation effect, and 3 bottles of the same repetition were set in each group for determination of physiological indicators. The preservation treatment was carried out at room temperature, the average laboratory temperature was 25±2℃, and the relative humidity was 60%-80%.

[0032] Example 4 (T4)

[0033] The trimmed cut flower vases were inserted into a loose-mouthed vase containing 1L of preservative and placed in a sunny place indoors. The pH value of the preservative was maintained at 3-4. The preservative ingredients and concentrations were 30g·L-1 sucrose, 5ml·L-1 lemon juice, 5g·L-1 garlic juice, and 50mg·L-1 2,4-D. There were 3 bottles in each group (repeated 3 times) and 5 flowers in each bottle for observing the preservation effect. Each group had 3 bottles of the same repetition for physiological index determination. The preservation treatment was carried out at room temperature, with an average laboratory temperature of 25±2℃ and a relative humidity of 60%-80%.

[0034] Example 5 (T5)

[0035] The trimmed cut flower vases were inserted into a loose-mouthed vase containing 1L of preservative and placed in a sunny place indoors. The pH value of the preservative was maintained at 3-4. The preservative ingredients and concentrations were 30g·L-1 sucrose, 5ml·L-1 lemon juice, 5g·L-1 garlic juice, and 50mg·L-1 NAA. Each group had 3 bottles (repeated 3 times), and each bottle had 5 flowers for observation of the preservation effect. Each group had 3 bottles of the same repetition for determination of physiological indicators. The preservation treatment was carried out at room temperature, with an average laboratory temperature of 25±2℃ and a relative humidity of 60%-80%.

[0036] Example 6 (T6)

[0037] The trimmed cut flower vases were inserted into a loose-mouthed vase containing 1L of preservative and placed in a sunny place indoors. The pH value of the preservative was maintained at 3-4. The preservative ingredients and concentrations were 30g·L-1 sucrose, 5ml·L-1 lemon juice, 5g·L-1 garlic juice, and 50mg·L-1 6-BA. There were 3 bottles in each group (repeated 3 times), 5 flowers in each bottle, for observation of the preservation effect. Each group had 3 bottles of the same repetition for determination of physiological indicators. The preservation treatment was carried out at room temperature, with an average laboratory temperature of 25±2℃ and a relative humidity of 60%-80%.

[0038] Example 7 (T7)

[0039] The trimmed cut flower vases were inserted into a loose-mouthed vase containing 1L of preservative and placed in a sunny place indoors. The pH value of the preservative was maintained at 3-4. The preservative ingredients and concentrations were 30g·L-1 sucrose, 5ml·L-1 lemon juice, and 5g·L-1 garlic juice. No plant growth regulators were added. Each group had 3 bottles (repeated 3 times), 5 flowers per bottle, for observation of the preservation effect. Each group had 3 bottles of the same repetition for determination of physiological indicators. The preservation treatment was carried out at room temperature, with an average laboratory temperature of 25±2℃ and a relative humidity of 60%-80%.

[0040] Control group (CK)

[0041] The trimmed cut flowers were placed in a loose-mouthed vase filled with 1L of distilled water and placed in a sunny place indoors. Each group had 3 bottles (3 replicates) and 5 flowers in each bottle for observation of preservation effects. Each group had 3 bottles of the same replicate for determination of physiological indicators. The preservation treatment was carried out at room temperature, with an average laboratory temperature of 25±2℃ and a relative humidity of 60% to 80%.

[0042] Effects of different plant growth regulators on vase life and cut flower quality

[0043] The vase life is the number of days from the day the flower branch is inserted into the solution to the day before the petals lose water and wilt or the flower buds fall off and turn yellow, losing their ornamental value. The method for measuring the degree of spread is: measure the maximum horizontal diameter of the petals with a ruler every day, and take the average of the maximum values ​​of different branches of 5 flowers in each treatment as the degree of petal spread. The method for judging the degree of flower head bending is: observe the morphology of the flower stems and pedicels of daylily cut flowers every day. No bending phenomenon is upright; some buds are bent, which is easy to bend; buds are bent and some open flowers are bent, which is severe bending. The survey method of the number of open flowers: record the proportion of the number of flowers on each branch of daylily cut flowers to the total number of flowers on each branch at 9 o'clock every day. The proportion of flowering (%) = the number of open flowers per day / total number of flowers × 100.

[0044] Effects of different plant growth regulators on nutritional indexes of cut daylily buds

[0045] The largest flower buds on the day of 2, 4, 6, and 8 days after vase insertion were taken to determine the nutritional quality indicators of the flower buds. 4-5 flower buds were sampled for each treatment each time, and repeated 3 times. The soluble sugar content was determined by the anthrone colorimetric method; the VC content was determined by the molybdenum blue colorimetric method.

[0046] Effects of different plant growth regulators on the physiological process of senescence in cut daylily flowers

[0047] The petals of the flowers that opened on the same day were selected for determination of senescence physiological indicators at 2, 4, 6, and 8 days after vase insertion. Three flowers were sampled for each treatment each time, and the process was repeated three times. The MDA content was determined by the thiobarbituric acid (TBA) method; the SOD content was determined by the nitroblue tetrazolium (NBT) method; and the proline content was determined by the ninhydrin colorimetric method.

[0048] The experimental data were processed and analyzed using Excel software, and the SPSS variance analysis method was used for significant difference analysis.

[0049] Table 1 Effects of different plant growth regulators on vase life and cut flower quality of day lily in Examples 1 to 7 and the control group

[0050]

[0051] As shown in Table 1, different plant growth regulators have different effects on the vase life of daylily. Except for KT and 6-BA, the vase life of all treatments is extended to varying degrees compared with CK. The GA3 treatment has the longest life (9 days), which is 4 days longer than CK. The 2,4-D and NAA treatments are second in extending the vase life, extending 3 days compared with CK, and the IAA and hormone-free treatments are 2 days longer than CK, indicating that the IAA and hormone-free treatments are more effective in extending the vase life. In the KT and 6-BA treatments, the flower buds turned slightly yellow and lost water on the second day of treatment. On the third day, the flower buds basically did not grow and began to fall, indicating that the KT and 6-BA treatments inhibited the flowering and bud growth of vase-inserted daylily.

[0052] The flowering rate was the highest in the 2,4-D treatment, followed by the NAA treatment, the GA3 treatment was the third, and the IAA and basic preservative treatments were the fourth, all of which were higher than CK. However, the flowering rates of the KT and 6-BA treatments were lower than those of the control. After 6 days of vase insertion, only the flower buds treated with GA3 and NAA remained normal. Except for KT and 6-BA, the flower buds were not easy to fall off after the growth regulator treatment, while the flower buds were more likely to dry and fall off after the basic preservative and water treatment.

[0053] Comprehensive observation results: After IAA treatment of cut daylily flowers, the flower buds remained normal, the flowering period was short, and the petals were easy to close; GA3 treatment promoted flowering, prolonged the flowering period, the flower buds were green, the vase life was the longest, and the nutrient solution remained clear; after KT treatment, the flower buds quickly wilted, shriveled, aged prematurely, and the preservative solution was turbid; 2,4-D promoted the opening of effective flower buds of daylily, the flowering period was long, the incision of the flower stalk turned yellow and rotten, and the base of the flower buds was easy to bend; NAA promoted the opening of effective flower buds of daylily, the flower buds had normal color, a long flowering period, and the base of the flower buds was bent; 6-BA flower buds wilted, shriveled, aged prematurely, and the liquid was turbid; after basic preservative treatment, the flower buds maintained normal size, were easy to fall off, the petals became narrower, and were not easy to open; while the buds fell early, the flowering period was short, and the solution was clear when treated with clean water.

[0054] Daylily is a vegetable that can be used for both viewing and eating, so the quality of daylily buds is also an important evaluation indicator of the preservation effect of cut flowers. Figure 1 It can be seen that, except for KT and 6-BA treatments, the bud weights of all growth regulator treatments were higher than those of the control water treatment. On the second day of vase placement, the bud weights were close to or slightly higher than those of the control. After the fourth day of vase placement, GA3, 2,4-D and NAA could still maintain the same initial bud weight, while the IAA treatment, although higher than the control water treatment, decreased significantly compared with the initial bud weight. After six days of vase placement, although the bud weights were higher than those of the control water treatment, all growth regulator treatments decreased significantly compared with the initial bud weight. The bud weights all showed a downward trend with the increase of vase placement days, among which the KT and 6-BA treatments had the largest decrease.

[0055] Depend on Figure 2It can be seen that, except for the KT and 6-BA treatments, the flower bud length of each treatment of daylily in vase was higher than that of the control water treatment, and showed a slow downward trend with the increase of vase days. Among them, the flower bud length of IAA treatment decreased significantly from the initial value starting on the 6th day of vase, while the flower bud length of GA3, 2,4-D, NAA and basic preservative treatments only showed a significant decrease on the 8th day of vase, and the decrease was obvious on the tenth day. The flower bud length of KT and 6-BA treatments was smaller than that of the control water treatment starting on the 2nd day of vase, and showed a very significant downward trend with the number of vase days. According to the observation data, the flower buds wilted, yellowed, and fell off, indicating that the KT and 6-BA treatments inhibited the growth of fresh-cut daylily buds, and the vase effect was not ideal.

[0056] The flowering period of daylily is one day. Whether it can continue to bloom normally after cut flower cultivation is an important indicator to determine the preservation effect of fresh cut flowers. Figure 3 It can be seen that the KT and 6-BA treatments stopped blooming after 2 days of vase placement, and the control water treatment stopped blooming after 4 days of vase placement. For the basic preservative treatment, the flower length showed a wave-like change with the vase placement time. Except for 2,4-D, the flower length of cut flowers treated with other growth regulators showed a downward trend with the vase placement time, and the flower length of cut flowers was higher than that of the control water treatment. The flower length of IAA treatment remained at a large value within 6 days after vase placement, but it stopped blooming after 6 days.

[0057] Depend on Figure 3 , 4 It can be seen that all treatments stopped blooming after 6 days of vase placement, and the degree of cut flower development was 0. On the 8th day of vase placement, the flowers of GA3, NAA, 2,4-D and basic preservatives were elongated but not opened. After 2 days of vase placement, the flowers of KT and 6-BA treatments did not elongate or open. The treatments of various growth regulators increased first and then decreased with the number of days of vase placement, and were all higher than the control treatment. On the 2nd day of vase placement, the degree of cut flower development of each treatment was not much different. On the 4th day of vase placement, the degree of cut flower development of each treatment was significantly different, with NAA being the highest, followed by 2,4-D, GA3, basic preservatives, and IAA. On the 6th day of vase placement, the cut flowers of the control water treatment no longer grew and opened, and the degree of cut flower development of other treatments was still significantly different, with NAA being the highest, followed by GA3, basic preservatives, 2,4-D, and IAA. This shows that 2,4-D treatment can promote the elongation of daylily flowers, and NAA treatment can promote the opening of daylily cut flowers.

[0058] Depend on Figure 5It can be seen that except for KT and 6-BA treatments, the single weight of cut daylily flowers with aborted flowers in other treatments was higher than that in the control. Among them, the single weight of aborted flowers in 2,4-D, IAA, GA3 and NAA treatments showed a trend of first rising and then falling, while the basic preservatives and the control treatments showed a gradual downward trend. In the 2,4-D treatment, the single weight of aborted flowers remained the highest after 2 days of vase placement. Combined with the length and development of cut flowers, it is believed that the 2,4-D treatment can promote the elongation of cut flowers and the opening of effective flower buds. NAA can promote the opening of effective flower buds of daylily and prolong the flowering period; after IAA treatment, the cut daylily can promote the elongation of flower buds, but the flowering period is short and the petals are easy to close; GA3 treatment can promote the elongation and opening of cut flowers, with a long flowering period, green flower buds and the longest vase life; after KT and 6-BA treatment, the growth of flower buds is inhibited, resulting in water loss in cut flowers, rapid wilting, shriveling and premature aging of flower buds.

[0059] Soluble sugar is one of the most important nutrients in cut flowers, reflecting the nutritional physiology of cut flowers. Figure 6 It can be seen that the soluble sugar content of each plant growth regulator treatment during the vase period was higher than that of the control. Except for the 2,4-D treatment which showed a trend of decreasing, then increasing and then decreasing, the other treatments generally showed a trend of decreasing first and then increasing. All of them can improve the nutritional physiology of daylily cut flowers, with the basal nutrient solution and GA3 treatments having the highest soluble sugar content. GA3 had the best improvement effect after 6 days of vase insertion.

[0060] VC is an indispensable small molecule organic compound for maintaining plant life activities, participating in the redox reaction in plants and an important indicator of vegetable quality. Figure 7 It can be seen that the VC content of the buds of the control and various treatments of cut daylily flowers fluctuated in the range of 40-80 mg / 100 g, but compared with the control, the basic preservatives and various growth regulators had no significant effect on improving the antioxidant effect of cut daylily flowers.

[0061] Malondialdehyde (MDA) is one of the main products of membrane lipid peroxidation, and its content reflects the degree of damage to plant organs. The higher the MDA content, the stronger the degree of membrane lipid peroxidation and the faster the plant aging. Figure 8 It can be seen that the MDA content of the control and the treatments with various plant growth regulators during the vase period has different trends. The control shows an upward trend, while the other plant growth regulator treatments show a trend of first decreasing and then increasing. The MDA content of the vase control is significantly higher than that of the various plant growth regulator treatments, indicating that the plant growth regulator treatment can effectively protect the cut flower petals from peroxidation damage. The overall MDA content of the GA3 treatment is low, indicating that its antioxidant capacity is good. Without plant growth regulator treatment, the MDA content is the lowest on the 4th day of vase insertion and the highest on the 6th day of vase insertion, indicating that the basic nutrient solution can alleviate peroxidation damage in the short term, but the antioxidant capacity in the later period is worse than that of the plant growth regulator.

[0062] SOD can remove active oxygen in plants and protect cell membranes. Its activity is related to plant resistance to disease, stress, and aging. Fig. 9 It can be seen that the SOD content of the basic preservative treatment and NAA treatment showed a trend of first increasing and then decreasing with the vase insertion time, and the SOD content of the 2,4-D, GA3, and control water treatment showed a trend of first decreasing and then increasing with the vase insertion time. In addition, the SOD content of the 2,4-D and GA3 treatments on the 2nd and 6th days of vase insertion were higher than those of the basic preservative treatment, NAA treatment, and control water treatment, indicating that 2,4-D and GA3 can increase the activity of SOD, thereby improving the ability of daylily cut flowers to scavenge free radicals. The preservative treatment containing 2,4-D had the best effect, followed by the preservative effect of GA3, which is consistent with the fact that the cut flowers had the longest vase insertion life of the two treatments.

[0063] Increased proline content is a sign that plants are damaged by stress. Fig.10 It can be seen that during the vase period, the proline content of the control was generally higher than that of the plant growth regulator treatment. After vase insertion, the control and IAA treatments showed a gradual upward trend, while the other growth regulator treatments showed a trend of first decreasing and then increasing. After the fourth day of vase insertion, the increase rate increased rapidly. The GA3 treatment was always lower than the other treatments, indicating that the cut daylily flowers treated with GA3 were less affected by water stress.

[0064] Therefore, the basic preservative can extend the vase life of cut daylily flowers, promote the growth of flower buds, and the vase life is 2 days longer than the control, but the buds fall seriously, the petals become narrower, and it is not easy to open. 50mg·L-1GA3 treatment can extend the vase life of cut daylily flowers, promote the growth and opening of flower buds, maintain the color and normal growth of flower buds, reduce the content of MDA, reduce the degree of membrane lipid peroxidation, maintain cell activity, increase SOD activity, reduce physiological stress after cutting flowers, and have the longest vase life. The preservative can remain clear. 50mg·L-12,4-D treatment can promote the growth and opening of flower buds of cut daylily flowers, the cut flowers have the largest development, the long flowering period, and the single flower withered is heavy. However, as the number of days in the vase increases, the base of the flower buds bends, affecting the quality of the cut flowers. After the cut daylily flowers are treated with 50mg·L-1IAA, the flower buds remain normal and promote the elongation of the flowers, but the development is not high, the flowering period is short, and the petals are easy to close. 50mg·L-1NAA can promote the growth and opening of effective flower buds of daylily, the color of flower buds is normal, the length and opening degree of flowers are good in the early stage, the flowering period is long, the proline content is reduced, and the water stress after cutting is reduced, but the base of the flower buds is easy to bend. After treatment with 50mg·L-1KT and 50mg·L-16-BA, the flower buds wilt rapidly, become shrunken, and prematurely age, and the preservative is easy to become turbid, which is not suitable for the preservation of cut daylily flowers.

[0065] In summary, 50mg·L-1GA 3Treatment can extend the vase life of cut daylily flowers, promote the elongation and opening of flower buds, reduce water stress and membrane lipid oxidation, and achieve the best preservation effect.

[0066] Although the above describes the specific implementation methods of the present invention in combination with the embodiments, it is not intended to limit the scope of protection of the present invention. Technical personnel in the relevant field should understand that various modifications or variations that can be made by technical personnel in the field without creative work on the basis of the technical solution of the present invention are still within the scope of protection of the present invention.

Claims

1. A method for preserving cut daylily flowers, characterized in that: The following steps are involved:

1. Flower cutting time: After the first flower bud reaches the picking standard, start cutting the flower stalk; 2. Treatment before vase insertion: According to the height of the vase, cut off the excess flower stalks, preferably with the base of the lowest inflorescence axis equal to or higher than 10cm from the vase mouth, and use a utility knife with the blade wiped clean with 75% alcohol to cut the flower stalk at a 45° angle; 3. Fresh-keeping treatment: Insert the treated flower stalk into a vase filled with fresh flower preservative solution. The height of the vase is 25-35cm. To extend the vase life, the fresh flower preservative solution can be replaced every 3-4 days, and the flower branches are placed in a well-lit place for cultivation; The pH value of the preservative is maintained at 3-4, and the preservative composition and concentration are sucrose 30g·L -1 , lemon juice 5ml·L -1 , Garlic juice 5g·L -1 , 50mg·L -1 GA 3, 3 bottles per group, 5 flowers per bottle, for observation of preservation effect, 3 bottles per group for determination of physiological indexes, preservation treatment was carried out at room temperature, the average laboratory temperature was 25±2℃, relative humidity was 60%~80%.

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