A kind of sterile instant undenatured natural collagen freeze-dried product and its preparation method

Abstract

The invention provides a sterile instant undenatured natural collagen freeze-dried product and a preparation method thereof. The preparation method includes the steps that (1) an active collagen stocksolution with a certain concentration is prepared, a blocking agent is added while stirring is conducted, the stirring time is 0.5-2 hours, the reaction temperature is 10-20 DEG C, then an instantizer is added, the stirring time is 0.5-2 hours, the reaction temperature is 10-20 DEG C, and a reaction pH is controlled to be 5-7; and (2) after a reaction, the instant undenatured natural collagen freeze-dried product is obtained after filling and freeze-drying. The collagen freeze-dried product retains the structural properties of natural undenatured collagen, and can be dissolved in water for 1-2 min without acylation treatment, no thickening agent is added to the collagen freeze-dried product, after being dissolved with the water, a thick skin feeling is achieved, and the product is safer;and the collagen freeze-dried product is subjected to terminal sterilization by using a low-dose electron beam irradiation sterilization process, and is a sterile product, and the natural activity ofthe collagen is still remained after sterilization.

Description

technical field

[0001] The invention relates to a sterile instant undenatured natural collagen freeze-dried product and a preparation method. Background technique

[0002] Collagen, or collagen, is widely distributed in various animals and is the main structural protein in animals, accounting for about 30% of the total protein in the body. At present, 28 kinds of collagens have been found in vertebrates, among which type I collagen is the most abundant, accounting for about 90% of the total collagen in animals. Collagen exists widely in connective tissues such as skin, bone, tendon and so on of pigs, cattle, sheep and other mammals, which provides the possibility for its large-scale application. The unique triple helical structure of collagen endows it with many excellent properties, such as good mechanical properties, low antigenicity and good biocompatibility, which is conducive to the survival and growth of cells, and also has moisturizing properties and obvious promotio...

Examples

Embodiment 1

[0027] A preparation method of a sterile instant undenatured natural collagen freeze-dried product is as follows:

[0028] 1. Prepare 100g of active collagen stock solution with a concentration of 0.1% in mass percentage, add 0.1g of mannitol while stirring, the stirring time is 1 hour, and the reaction temperature is 10°C, then add 0.1g of disodium hydrogen phosphate, and the stirring time is 0.5 hours , the reaction temperature is 10°C. Control the reaction pH to about 5.0.

[0029] 2. Stop stirring, take samples, test the pH value, and conduct microbial detection. After passing the test, fill it into a spherical mold and freeze-dry to obtain instant undenatured natural collagen freeze-dried balls.

[0030] 3. The lyophilized spheres are sterilized and irradiated with an electron beam at a dose of 6 kGy. The sterile instant undenatured natural collagen freeze-dried product is obtained.

Embodiment 2

[0032] A preparation method of a sterile instant undenatured natural collagen freeze-dried product is as follows:

[0033] 1. Prepare 100g of active collagen stock solution with a concentration of 0.2% in mass percentage, add 0.2g of mannitol while stirring, the stirring time is 1.5 hours, and the reaction temperature is 15°C, then add 0.2g of disodium hydrogen phosphate, and the stirring time is 1 hour , and the reaction temperature was 15°C. Control the reaction pH to about 6.0.

[0034] 2. Stop stirring, take samples, test the pH value, and conduct microbial detection. After passing the test, fill it into a spherical mold and freeze-dry to obtain instant undenatured natural collagen freeze-dried balls.

[0035] 3. The freeze-dried balls are sterilized and irradiated with an electron beam, and the dose is 8kGy. The sterile instant undenatured natural collagen freeze-dried product is obtained.

Embodiment 3

[0037] A preparation method of a sterile instant undenatured natural collagen freeze-dried product is as follows:

[0038] 1. Prepare 100g of active collagen stock solution with a concentration of 0.3% by mass, add 0.3g of mannitol while stirring, the stirring time is 2 hours, the reaction temperature is 20°C, then add 0.3g of disodium hydrogen phosphate, and the stirring time is 2 hours , the reaction temperature is 20°C. Control the reaction pH to about 7.

[0039] 2. Stop stirring, take samples, test the pH value, and conduct microbial detection. After passing the test, fill it into a spherical mold and freeze-dry to obtain instant undenatured natural collagen freeze-dried balls.

[0040] 3. The lyophilized spheres are sterilized and irradiated with an electron beam at a dose of 10 kGy. The sterile instant undenatured natural collagen freeze-dried product is obtained.

technical field

[0001] The invention relates to a sterile instant undenatured natural collagen freeze-dried product and a preparation method. Background technique

[0002] Collagen, or collagen, is widely distributed in various animals and is the main structural protein in animals, accounting for about 30% of the total protein in the body. At present, 28 kinds of collagens have been found in vertebrates, among which type I collagen is the most abundant, accounting for about 90% of the total collagen in animals. Collagen exists widely in connective tissues such as skin, bone, tendon and so on of pigs, cattle, sheep and other mammals, which provides the possibility for its large-scale application. The unique triple helical structure of collagen endows it with many excellent properties, such as good mechanical properties, low antigenicity and good biocompatibility, which is conducive to the survival and growth of cells, and also has moisturizing properties and obvious promotio...

Examples

Embodiment 1

[0027] A preparation method of a sterile instant undenatured natural collagen freeze-dried product is as follows:

[0028] 1. Prepare 100g of active collagen stock solution with a concentration of 0.1% in mass percentage, add 0.1g of mannitol while stirring, the stirring time is 1 hour, and the reaction temperature is 10°C, then add 0.1g of disodium hydrogen phosphate, and the stirring time is 0.5 hours , the reaction temperature is 10°C. Control the reaction pH to about 5.0.

[0029] 2. Stop stirring, take samples, test the pH value, and conduct microbial detection. After passing the test, fill it into a spherical mold and freeze-dry to obtain instant undenatured natural collagen freeze-dried balls.

[0030] 3. The lyophilized spheres are sterilized and irradiated with an electron beam at a dose of 6 kGy. The sterile instant undenatured natural collagen freeze-dried product is obtained.

Embodiment 2

[0032] A preparation method of a sterile instant undenatured natural collagen freeze-dried product is as follows:

[0033] 1. Prepare 100g of active collagen stock solution with a concentration of 0.2% in mass percentage, add 0.2g of mannitol while stirring, the stirring time is 1.5 hours, and the reaction temperature is 15°C, then add 0.2g of disodium hydrogen phosphate, and the stirring time is 1 hour , and the reaction temperature was 15°C. Control the reaction pH to about 6.0.

[0034] 2. Stop stirring, take samples, test the pH value, and conduct microbial detection. After passing the test, fill it into a spherical mold and freeze-dry to obtain instant undenatured natural collagen freeze-dried balls.

[0035] 3. The freeze-dried balls are sterilized and irradiated with an electron beam, and the dose is 8kGy. The sterile instant undenatured natural collagen freeze-dried product is obtained.

Embodiment 3

[0037] A preparation method of a sterile instant undenatured natural collagen freeze-dried product is as follows:

[0038] 1. Prepare 100g of active collagen stock solution with a concentration of 0.3% by mass, add 0.3g of mannitol while stirring, the stirring time is 2 hours, the reaction temperature is 20°C, then add 0.3g of disodium hydrogen phosphate, and the stirring time is 2 hours , the reaction temperature is 20°C. Control the reaction pH to about 7.

[0039] 2. Stop stirring, take samples, test the pH value, and conduct microbial detection. After passing the test, fill it into a spherical mold and freeze-dry to obtain instant undenatured natural collagen freeze-dried balls.

[0040] 3. The lyophilized spheres are sterilized and irradiated with an electron beam at a dose of 10 kGy. The sterile instant undenatured natural collagen freeze-dried product is obtained.

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