Embryo observation device

Abstract

The invention discloses an embryo observation device, comprising a workbench, a fixing fixture and a measuring mechanism, wherein the fixing fixture is installed on the workbench, a through hole is opened on the fixing fixture, and the through hole is used to fix a culture dish, and the measuring mechanism comprises a driving component, a microscope and a light-emitting component, wherein the microscope is located below the fixing fixture, the light-emitting component is located above the fixing fixture, a line connecting the microscope and the light-emitting component is vertically pointed to the through hole, the microscope is fixed on the driving component, the light-emitting component is fixed on the driving component, a moving end of the driving component drives the microscope and the light-emitting component to translate synchronously, and a telescopic end of the driving component drives the microscope to move toward or away from the light-emitting component; the problem that the prior art is time-consuming, labor-intensive, inefficient and difficult to meet the needs when performing long-term continuous measurement on a large number of embryos is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of embryo culture, and in particular to an embryo observation device. Background Art

[0002] Currently, during the embryo culture process, a microscope is needed to observe the embryo in order to determine the embryo's growth status. The existing observation method is to manually place the embryo under a microscope for observation. However, when conducting long-term continuous observation on large quantities of embryos, it is time-consuming, labor-intensive, inefficient, and difficult to meet demand. Summary of the invention

[0003] The purpose of the present invention is to overcome the above technical deficiencies and provide an embryo observation device to solve the problem in the prior art that when a large number of embryos need to be continuously measured for a long time, it is time-consuming and labor-intensive, inefficient and difficult to meet the needs.

[0004] To solve the above technical problems, the present invention provides an embryo observation device, comprising a workbench, a fixing fixture and a measuring mechanism, wherein the fixing fixture is installed on the workbench, a through hole is opened on the fixing fixture, and the through hole is used to fix the culture dish, and the measuring mechanism comprises a driving component, a microscope and a light-emitting component, the microscope is located below the fixing fixture, the light-emitting component is located above the fixing fixture, a line connecting the microscope and the light-emitting component is vertically pointed to the through hole, the microscope is fixed on the driving component, the light-emitting component is fixed on the driving component, the moving end of the driving component drives the microscope and the light-emitting component to translate synchronously, and the telescopic end of the driving component drives the microscope to move toward or away from the light-emitting component.

[0005] The beneficial effects of the present invention are as follows: different from the prior art, the present invention arranges a fixing fixture, on which a plurality of culture dishes can be placed at one time; specifically, the light source is provided by irradiating the embryo through a light-emitting component, and the embryo can be observed through a microscope; after observing one embryo, a driving component is arranged, and a moving belt of the driving component can carry the microscope and the light-emitting component workbench to translate synchronously, so that the growth condition of the embryo in the next culture dish can be observed, which saves time and effort; and by arranging the driving component, the telescopic end of the driving component can carry the microscope to move toward or away from the light-emitting component, so that the distance from the microscope to the light source can be adjusted, so as to adjust the focal length, and observe different levels of the embryo; the embryo does not need to be moved to observe the embryo; and after observing one embryo, the next embryo can be quickly found through the driving component for observation, which is convenient and quick. BRIEF DESCRIPTION OF THE DRAWINGS

[0006] Figure 1 It is a schematic diagram of the structure of the embryo measuring device of the present invention;

[0007] Figure 2 It is a front view of the present invention;

[0008] Figure 3 It is a side view of the present invention. DETAILED DESCRIPTION

[0009] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0010] like Figure 1 As shown, the present invention provides an embryo observation device, including a workbench 100, a fixing fixture (not shown in the figure) and a measuring mechanism 300. During implementation, the measuring mechanism 300 and the fixing fixture are both installed on the workbench 100, wherein the fixing fixture is fixed relative to the workbench 100, and multiple culture dishes can be placed in the fixing fixture. Specifically, the measuring mechanism 300 moves relative to the culture chamber 200 to facilitate the measuring end of the measuring mechanism 300 to approach the culture dish on the fixing fixture. After observing one of the culture dishes, the next culture dish can be accurately found, which is explained in more detail below.

[0011] like Figure 1 As shown, the workbench 100 in this embodiment includes a base 110, a plurality of brackets 120 and a support plate 130. The base 110 and the support plate 130 are arranged in parallel, and the plurality of brackets 120 are evenly distributed around the base 110. Each bracket 120 is in a T-structure, and one end is connected to the base 110, and the other end of each bracket 120 is connected to the support plate 130.

[0012] Both the base 110 and the support plate 130 are flat plates, wherein the height of the bracket 120 should be sufficient to allow the microscope 320 to adjust its relative position with the fixing fixture in the vertical direction, so as to facilitate the adjustment of the microscope 320. It can be understood that the gap between the base 110 and the support is the size of the space in which the microscope 320 can move, and the shapes of the base 110 and the support plate 130 can also be replaced by other forms of structures.

[0013] The fixing fixture in this embodiment is installed on the workbench 100. A through hole is opened on the fixing fixture, and the through hole is used to fix the culture dish. It can be understood that the fixing fixture can also be replaced by other forms of mechanisms, and it can only fix multiple culture dishes between the microscope 320 and the light-emitting component 330.

[0014] Preferably, the fixing fixture is a culture chamber 200, which includes a fixing frame 210 and a chamber body 220. The chamber body 220 is slidably connected to a slide groove provided on the side wall of the fixing frame 210. The chamber body 220 has a channel for gas inflow and outflow, through which a gas mixed with a good ratio of CO2 and O2 can be introduced to maintain a constant pH value and oxygen concentration. The heating glass under the chamber body 220 can maintain a constant temperature of the culture chamber 220, thereby ensuring the long-term survival of the embryo.

[0015] More preferably, there may be multiple cabins 220, which correspond one-to-one to the multiple through holes opened on the side wall of the fixed frame 210. Multiple culture dishes can be placed in one cabin 220, and the cabin 220 is provided with through holes for snapping in the culture dishes.

[0016] Among them, the cabin 220 is frame-shaped, the opening above the cabin 220 faces the transparent glass, the opening below the cabin 220 is installed with transparent heating glass and a temperature sensor, the top of the fixed frame 210 is provided with a mounting hole, transparent glass is placed in the mounting hole, the mounting hole faces the culture dish in the cabin 220, and the bottom of the fixed frame 210 has an opening, and the opening faces the heating glass.

[0017] It can be understood that the fixed frame 210 and the cabin body 220 are connected in a drawer-like manner, and the transparent glass installed above the fixed frame 210 is facing the culture dish in the cabin body 220. When the light-emitting component 330 is illuminated, the light-emitting component 330 sequentially illuminates the transparent glass, the embryo in the culture dish, and the heating glass until it is illuminated into the microscope 320, thereby facilitating observation.

[0018] Furthermore, the culture chamber 200 can provide the embryo with a desired growth environment. After culturing the embryo, observation can be completed without moving the embryo, saving time and effort. Therefore, using the culture chamber 200 as a fixed fixture is the most preferred solution in this embodiment.

[0019] like Figure 1 As shown, the measuring mechanism 300 in this embodiment includes a driving component 310, a microscope 320 and a light-emitting component 330. The microscope 320 is located below the fixing fixture, and the light-emitting component 330 is located above the fixing fixture. The line connecting the microscope 320 and the light-emitting component 330 points vertically to the through hole. The microscope 320 is fixed on the driving component 310, and the light-emitting component 330 is fixed on the driving component 310. The moving end of the driving component 310 drives the microscope 320 and the light-emitting component 330 to translate synchronously, and the telescopic end of the driving component 310 drives the microscope 320 to move toward or away from the light-emitting component 330.

[0020] like Figure 2-3As shown, the driving component 310 includes a moving seat 311, a lifting member 312, a first translation member 313 and a second translation member 314. One end of the lifting member 312 is connected to the moving seat 311, and the other end of the lifting member 312 is connected to the microscope 320. The lifting member 312 drives the microscope 320 to move up and down. The light-emitting component 330 is fixed on the moving seat 311 and is located directly above the microscope 320. One end of the first translation member 313 is connected to the moving seat 311, and the output end of the first translation member 313 drives the moving seat 311 to move horizontally. The other end of the first translation member 313 is connected to the second translation member 314, and the second translation member 314 is installed on the base 110. The output end of the second translation member 314 drives the first translation member 313 to move horizontally, and the movement directions of the output end of the first translation member 313 and the output end of the second translation member 314 are perpendicular to each other.

[0021] Preferably, the lifting member 312 includes a motor, a screw, a fixed cylinder and a threaded tube. The motor and the fixed cylinder are both installed on the second translation member 314. The output shaft of the motor is connected to one end of the screw, and the other end of the screw extends into the threaded tube. The inner wall of the threaded tube is threadedly connected to the screw, and the end of the threaded tube away from the motor is connected to the microscope 320, and the outer wall of the threaded tube is slidably connected to the inner wall of the fixed cylinder.

[0022] Preferably, the first translation member 313 includes a first motor, a first guide rail and a first mounting block, the first motor and the first mounting block are both mounted on the first guide rail, the first guide rail is fixed on the second translation member 314, the telescopic end of the first motor is connected to the first mounting block, the first mounting block is slidably connected to the first guide rail, and the lifting member 312 is fixed on the first mounting block.

[0023] Preferably, the second translation member 314 includes a second motor, a second guide rail and a second mounting block, the second motor and the second mounting block are both mounted on the second guide rail, the second guide rail is fixed on the base 110, the telescopic end of the second motor is connected to the second mounting block, the second mounting block is slidably connected to the second guide rail, and the first translation member 313 is fixed on the second mounting block.

[0024] It can be understood that the lifting member 312, the first translation member 313 and the second translation member 314 constitute the three output ends of the three-axis linkage driving component 310. Specifically, the moving directions of the first translation member 313 and the second translation member 314 are perpendicular to each other, driving the microscope 320 and the light-emitting component 330 to translate to any position on the base 110. The movement direction of the telescopic end of the lifting member 312 is perpendicular to the base 110, thereby driving the microscope 320 to move toward or away from the light-emitting component 330.

[0025] Preferably, the microscope 320 is a phase contrast microscope, and the output end of the microscope 320 can be externally connected to a CCD.

[0026] Preferably, the light-emitting component 330 includes a cold light LED lamp and a lampshade. The cold light LED lamp is installed in the lampshade. The lampshade is fixed on the driving component 310. The opening direction of the lampshade points to the fixing clamp. The cold light LED lamp can reduce the influence of the heat generated by the light-emitting component 330 on the embryonic growth. The lampshade can specify the lighting direction of the cold light LED to avoid the influence on the surrounding environment.

[0027] Workflow: insert multiple culture dishes containing embryos into multiple through holes on the fixing fixture. If the fixing fixture adopts a culture cabin 200, pull out the cabin body 220 from the fixing frame 210, put multiple culture dishes into the cabin body 220 in turn, insert the cabin body 220 into the fixing frame 210, turn on the first motor and the second motor, drive the microscope 320 and the light-emitting component 330 to move relative to the base 110, so that the microscope 320 and the light-emitting component 330 are facing one of the culture dishes at the same time, turn on the motor, the motor drives the lead screw to rotate, the lead screw is threadedly connected to the threaded rod, so as to adjust the microscope 320 connected to the threaded tube to move up and down until the microscope 320 moves to a predetermined distance from the corresponding culture dish, turn on the light-emitting component 330, and the light-emitting component 330 sequentially irradiates the transparent glass, the embryo in the culture dish, and the heating glass until it is irradiated into the microscope 320, which is convenient for observation. The microscope 320 can continue to be moved up and down to observe the growth of embryos at different levels. After the observation, repeat the above steps to observe the next culture dish.

[0028] Different from the prior art, the present invention sets a fixing fixture on which a plurality of culture dishes can be placed at one time. Specifically, the light source is provided by irradiating the embryo through the light-emitting component 330, and the embryo can be observed through the microscope 320. After observing one embryo, the driving component 310 is set, and the moving belt of the driving component 310 can move synchronously with the microscope 320 and the light-emitting component 330 on the workbench 100, so that the growth condition of the embryo in the next culture dish can be observed, which saves time and effort. Moreover, by setting the driving component 310, the telescopic end of the driving component 310 can move the microscope 320 toward or away from the light-emitting component 330, so that the distance from the microscope 320 to the light source can be adjusted, thereby adjusting the focal length, and observing different levels of the embryo. There is no need to move the embryo to observe the embryo, and after observing one embryo, the next embryo can be quickly found through the driving component 310 for observation, which is convenient and quick.

[0029] It should be noted that the above embodiments all belong to the same inventive concept, and the description of each embodiment has its own focus. For matters that are not described in detail in some embodiments, reference may be made to the description in other embodiments.

[0030] The above-mentioned embodiments only express the implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. An embryo observation device, characterized in that: It comprises a workbench, a fixing fixture and a measuring mechanism, wherein the fixing fixture is installed on the workbench, a through hole is formed on the fixing fixture, and the through hole is used to fix the culture dish, and the measuring mechanism comprises a driving component, a microscope and a light-emitting component, wherein the microscope is located below the fixing fixture, and the light-emitting component is located above the fixing fixture, and a line connecting the microscope and the light-emitting component is vertically pointed to the through hole, the microscope is fixed on the driving component, and the light-emitting component is fixed on the driving component, and the moving end of the driving component drives the microscope and the light-emitting component to translate synchronously, and the telescopic end of the driving component drives the microscope to move toward or away from the light-emitting component; The fixing fixture is a culture chamber, which includes a fixing frame and a chamber body, the chamber body is slidably connected to a slide groove provided on a side wall of the fixing frame, and the chamber body has a gas inflow and outflow channel; There are multiple cabins, and the multiple cabins correspond to the multiple through holes opened on the side wall of the fixed frame one by one. Multiple culture dishes can be placed in one cabin, and the cabin is provided with through holes for the culture dishes to be clamped; The workbench includes a base, a plurality of brackets and a support plate, the base and the support plate are arranged in parallel, the plurality of brackets are evenly distributed around the base, each bracket is in a T-shaped structure, and one end is connected to the base, and the other end of each bracket is connected to the support plate; The cabin is frame-shaped, the opening above the cabin faces the transparent glass, the opening below the cabin is installed with a transparent heating glass and a temperature sensor, the top of the fixed frame is provided with a mounting hole, the transparent glass is placed in the mounting hole, the through hole faces the culture dish in the cabin, and the bottom of the fixed frame has an opening, the opening faces the heating glass; The driving component includes a moving seat, a lifting member, a first translation member and a second translation member, one end of the lifting member is connected to the moving seat, the other end of the lifting member is connected to the microscope, the lifting member drives the microscope to move up and down, the light-emitting component is fixed on the moving seat and is located directly above the microscope, one end of the first translation member is connected to the moving seat, and the output end of the first translation member drives the moving seat to move laterally, the other end of the first translation member is connected to the second translation member, the second translation member is installed on the base, and the output end of the second translation member drives the first translation member to move laterally, and the movement directions of the output ends of the first translation member and the second translation member are perpendicular to each other.

2. An embryo observation device according to claim 1, characterized in that: The lifting member includes a motor, a screw rod, a fixed cylinder and a threaded tube. The motor and the fixed cylinder are both installed on the second translation member. The output shaft of the motor is connected to one end of the screw rod, and the other end of the screw rod extends into the threaded tube. The inner wall of the threaded tube is threadedly connected to the screw rod, and the end of the threaded tube away from the motor is connected to the microscope, and the outer wall of the threaded tube is slidably connected to the inner wall of the fixed cylinder.

3. An embryo observation device according to claim 2, characterized in that: The first translation member includes a first motor, a first guide rail and a first mounting block, the first motor and the first mounting block are both mounted on the first guide rail, the first guide rail is fixed on the second translation member, the telescopic end of the first motor is connected to the first mounting block, the first mounting block is slidably connected to the first guide rail, and the lifting member is fixed on the first mounting block.

4. The embryo observation device according to claim 1, characterized in that: The second translation member includes a second motor, a second guide rail and a second mounting block, the second motor and the second mounting block are both mounted on the second guide rail, the second guide rail is fixed on the base, the telescopic end of the second motor is connected to the second mounting block, the second mounting block is slidably connected to the second guide rail, and the first translation member is fixed on the second mounting block.

5. The embryo observation device according to claim 1, characterized in that: The microscope is a phase contrast microscope.

6. The embryo observation device according to claim 1, characterized in that: The light-emitting assembly comprises a cold light LED lamp and a lampshade. The cold light LED lamp is installed in the lampshade. The lampshade is fixed on the driving assembly, and the opening direction of the lampshade points to the fixing fixture.

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