Seneca virus A immunogenic compositions and methods thereof

Abstract

The present invention relates to killed / inactivated and / or recombinant Senecavirus A immunogenic compositions and vaccines, and methods of using such immunogenic compositions and vaccines to prevent or treat animals in need thereof.

Description

[0001] Sequence Listing

[0002] This application contains a Sequence Listing according to 37 CFR 1.821-1.825. The Sequence Listing accompanying this application is incorporated herein by reference in its entirety. Background of the Invention

[0003] A. Technical field

[0004] The present invention relates to Seneca Valley virus A or Seneca virus A (SVA), and its use as an immunogenic composition or vaccine to treat animals infected with Seneca virus A.

[0005] B. Description of Related Technology

[0006] SVA is a non-enveloped, single-stranded, positive-sense RNA virus in the Picornaviridae family, which also includes foot-and-mouth disease virus (FMDV) and swine vesicular disease virus (SVDA).

[0007] The virus was originally discovered as a contaminant from cell culture medium (see Hales, LM et al., Complete genome sequence analysis of Seneca Valley virus-001, a novel oncolytic picornavirus. J Gen Virol, 2008. 89(Pt 5): 1265-75, incorporated herein by reference in its entirety). However, neutralizing antibodies against the virus have been detected in pigs, cattle, mice and humans (see Knowles, NJ et al., Epidemiology of Seneca Valley Virus: Identification and Characterization of Isolates from Pigs in the United States, in The Northern Lights EUROPIC 2006-14th meeting of the European Study Group on the Molecular Biology of Picornaviruses. 2006: Saairselka, Inari, Finland, incorporated herein by reference in its entirety). Clinical signs reported following infection include vesicular lesions on the snout and girdle, acute lameness, girdle ulcers, and hoof loss (Singh, K. et al., Seneca Valley Virus and vesicular lesions in a pig with idiopathic vesicular disease. J Vet Sci Technol, 2012. 3(6), and Pasma, T., S. Davidson and SL Shaw, Idiopathic vesicular disease in swine in Manitoba. CVJ, 2008. 49: pp. 84-85, both of which are incorporated herein by reference in their entireties). In 2016, Koch's postulate was realized when a cell culture-propagated SVA isolate was used to inoculate conventional animals and vesicular lesions were observed four days after inoculation (Montiel, N. et al., Vesicular Disease in 9-Week-Old Pigs Experimentally Infected with Senecavirus A. Emerg Infect Dis, 2016. 22(7): 1246-8, incorporated herein by reference in its entirety).

[0008] US 8,039,606 describes the use of Seneca Valley virus for treating tumors. However, this Seneca Valley virus is different from the SVA of the present invention.

[0009] There has been an unexplained increase in SVA cases in the United States, Canada, Australia, Italy, New Zealand, and Brazil. Furthermore, due to the similarity of clinical signs to FMDV, this virus has attracted interest from the swine industry. Center for Veterinary Biologics (CVB) Notice 16-03 confirms the CVB's interest in licensing biological products and / or prophylactic agents for SVA virus. Summary of the Invention

[0010] The present invention provides immunogenic compositions, vaccines, and related methods that overcome deficiencies in the art. The compositions and methods provide immunogenic compositions comprising inactivated / killed and / or recombinant forms of non-enveloped (+) single-stranded RNA viruses of SVA. In particular, the present application provides vaccines for generating an immune response in pigs for protection against diseases associated with Senecavirus A. The Senecavirus A isolate NAC#20150909 (SEQ ID NO: 1, SEQ ID NO: 2, and / or SEQ ID NO: 3) herein was isolated from vesicular fluid collected from 5-month-old pigs displaying vesicular lesions on the snout and coronary bands.

[0011] The immunogenic compositions and vaccines of the present invention comprise SEQ ID NO:1, SEQ ID NO:2 and / or SEQ ID NO:3.

[0012] An exemplary composition of the invention comprises the polypeptide sequence of SEQ ID NO: 3 or a fragment thereof that is immunoreactive with SVA.

[0013] The immunogenic compositions and vaccines of the present invention comprise an SVA antigen, which, in one non-limiting example, is expressed in insect cells via a recombinant baculovirus expressing modified SVA P1, 2A, portions 2B and 3B, and the 3C protease, such as a modified SVA nucleic acid sequence (SEQ ID NO: 18) encoding the amino acid sequence (SEQ ID NO: 19), and typically also include an adjuvant. Vaccines may also include other components, such as preservatives, stabilizers, and antigens against other porcine pathogens.

[0014] Preferred P1-2A-P3 nucleic acid sequences suitable for use in the present invention are polynucleotides encoding P1-2A-P3 polypeptides that have at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88% or 89% similarity to SEQ ID NOs: 18, 20, 22, 24, 32 and 33, more preferably at least 90%, 91%, 92%, 93% or 94%, and most preferably at least 95%, 96%, 96.1%, 96.2%, 96.3%, 96.4%, 96.5%, 96.6%, 96.7%, 96.8%, 96.9%, 97%, 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 97.1%, 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 97.1%, 97.1%, 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6 ... 7.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9% sequence identity. “As used herein, it is expressly understood that the terms “sequence identity to SEQ ID NO: X” or “identical SEQ ID NO: X” are equivalent to the terms “sequence identity to SEQ ID NO: X over the length of SEQ ID NO: X” or “sequence identity to SEQ ID NO: X over the length of SEQ ID NO: X”, respectively, wherein “X” in this context is any integer selected from 18, 20, 22, 24, 32 and 33.”

[0015] Preferred P1-2A-P3 polypeptides suitable for use in the present invention are polypeptides having the sequences shown in SEQ ID NOs: 19, 21, 23, 25, 27 and 29, having at least 80% homology to SEQ ID NOs: 19, 21, 23, 25, 27 and 29, such as at least 85% homology to SEQ ID NOs: 19, 21, 23, 25, 27 and 29, such as at least 85% homology to SEQ ID NOs: 19, 21, 23, 25, 27 and 29, such as at least 90% homology to SEQ ID NOs: 19, 21, 23, 25, 27 and 29, such as at least 95%, at least 98% or at least 99% homology to SEQ ID NOs: 19, 21, 23, 25, 27 and 29.

[0016] In another aspect, the present invention provides nucleic acid sequences encoding one or more polypeptides, antibody constructs, or antibody conjugates. The gene sequence encoding the polypeptide comprises a nucleic acid sequence that is at least 95%, 90%, 85%, or even 80% homologous and / or identical to SEQ ID NO: 1 or SEQ ID NO: 2, or a fragment thereof that encodes a polypeptide immunoreactive against SVA. Exemplary nucleic acid sequences of the present invention include any of SEQ ID NO: 1, SEQ ID NO: 2, and fragments thereof that encode polypeptides immunoreactive against SVA.

[0017] Furthermore, as used herein, polypeptides of the present invention include, but are not limited to, polypeptides comprising:

[0018] i) a polypeptide comprising the amino acid sequence of SEQ ID NO: 3;

[0019] ii) a polypeptide that is at least 80% homologous and / or identical to the polypeptide of i);

[0020] iii) fragments of the polypeptides of i) and / or ii);

[0021] iv) the polypeptide of i) or ii);

[0022] v) a fragment of iii) or iv), which comprises at least 5, preferably 8, more preferably 10, even more preferably 15 contiguous amino acids included in the sequence of SEQ ID NO: 3;

[0023] vi) a polypeptide encoded by a polynucleotide comprising the sequence of SEQ ID NO: 1 or 2;

[0024] vii) a polypeptide encoded by a polynucleotide that is at least 80% homologous or identical to the polynucleotide of vi);

[0025] viii) a protein fragment encoded by a polynucleotide comprising at least 15, preferably 24, more preferably 30, even more preferably 45 contiguous nucleotides comprised in the sequence of SEQ ID NO: 1 or SEQ ID NO: 2.

[0026] The immunogenic composition of the invention comprising at least one or more SVA polypeptides as defined herein may further comprise a physiologically acceptable vehicle, such as a pharmaceutically or veterinarily acceptable carrier, adjuvant or a combination thereof.

[0027] Any of the SVA polypeptides provided herein, or any immunogenic composition comprising one or more of these SVA polypeptides provided herein, can be used as a medicament, preferably as a vaccine or immunogenic composition, most preferably for the prevention or treatment of SVA infection in a subject.

[0028] Those skilled in the art will appreciate that the compositions used herein can be incorporated into known injectable, physiologically acceptable sterile solutions. In order to prepare ready-to-use solutions for parenteral injection or infusion, isotonic aqueous solutions, such as saline or plasma protein solutions, are readily available. In addition, the immunogenic and vaccine compositions of the present invention may include veterinarily acceptable carriers, diluents, isotonic agents, stabilizers, or adjuvants.

[0029] Methods of the present invention include, but are not limited to, methods of eliciting an immune response against SVA infection in a subject, comprising the step of administering to the subject an immunogenic composition comprising one or more SVA polypeptides as defined herein. The compositions of the present invention may be used to treat or, alternatively, prevent SVA infection. Preferably, such an immune response reduces the incidence or severity of one or more clinical symptoms associated with or caused by infection with an SVA serotype.

[0030] As used herein, suitable subjects and those to whom the compositions of the present invention are administered include animals in need of prevention or treatment of viral-related infections, diseases, or conditions. Animals in which an immune response is stimulated by using the compositions or methods of the present invention include livestock, such as pigs, cattle, goats, and sheep. Preferred animals include porcine, murine, equine, lagomorph, and bovine. Most preferably, an immune response is stimulated in pigs.

[0031] The present invention also provides a method for reducing the incidence or severity of one or more clinical signs associated with or caused by SVA infection, comprising the step of administering an immunogenic composition of the present invention comprising one or more SVA peptides as provided herein and preferably a carrier molecule, such that the incidence or severity of clinical signs of SVA infection is reduced by at least 10%, preferably at least 20%, even more preferably at least 30%, even more preferably at least 50%, even more preferably at least 70%, and most preferably at least 100%, relative to a subject that has not received the immunogenic composition as provided herein.

[0032] Such clinical signs include vesicular disease, such as open or closed blisters or lesions located on the snout, oral mucosa, and / or where the skin and hoof wall meet (coronary band), nail bed hemorrhages, sudden lameness with redness and swelling in or around the coronary band; and breeding females who suddenly stop eating, become lethargic, have anorexia, and / or have a fever as high as 105°F (~40.6°C).

[0033] There appears to be a short-term (4-10 day) increase in mortality in newborn piglets (less than 7 days old) which may or may not have associated diarrhea. Estimates of morbidity and mortality over this short period range from 30-70%. Vesicular lesions in breeding animals are often noted when investigating increases in neonatal mortality. This type of infection, which causes vesicles in the snout and coronary bands in pigs, has also been referred to as idiopathic vesicular disease in pigs.

[0034] According to a further aspect, the present invention also relates to a method for preventing SVA infection, wherein the SVA infection may be caused by Seneca Valley virus A, the method comprising the step of administering an immunogenic composition of the present invention comprising one or more SVA peptides as provided herein.

[0035] The present invention also provides a method for preparing any immunogenic composition provided herein, comprising mixing one or more SVA peptides as provided herein with a carrier molecule, preferably such that the one or more SVA peptides and the carrier molecule are covalently coupled or conjugated to each other. Such conjugates can be multivalent or monovalent. A multivalent composition or vaccine comprises an immunoconjugate of multiple SVA peptides with a carrier molecule. In a further aspect, the present invention provides a method for producing one or more SVA peptides, comprising transforming a host cell, preferably a prokaryotic cell, such as Escherichia coli (E. coli), with a nucleic acid molecule encoding any SVA peptide as provided herein. Alternatively, the host cell can be a eukaryotic cell, such as an animal cell, a protist cell, a plant cell, or a fungal cell. Preferably, the eukaryotic cell is a mammalian cell, such as CHO, BHK or COS, or a fungal cell, such as Saccharomyces cerevisiae, or an insect cell, such as Sf9.

[0036] Another aspect of the present invention provides a method for producing one or more SVA peptides that induce an immune response against SVA. This method comprises culturing a transformed expression vector encoding and expressing one or more SVA peptides disclosed herein. The expressed protein is either retained by the expressing organism or secreted into the culture medium. Expression is performed under conditions sufficient to produce an SVA peptide capable of inducing an immune response against SVA.

[0037] Also provided are methods for producing recombinantly expressed P1-2A-P3 antigen vaccines produced in insect cells via recombinant baculovirus expressing modified SVA P1-2A-P3 proteins. In one exemplary embodiment, the method comprises cloning the SVA P1-2A-P3 sequence into the vector pVL1393 (BD ​​Biosciences) and co-transfecting Sf9 insect cells. For inactivated recombinant SVA material, the SVA baculovirus harvest is inactivated for 24 hours using 5 mM BEI, clarified, and filtered through 0.45 μm. Typically, the inactivated virus is further processed, such as by concentration and blending with other components, to produce a commercial formulation. For example, the inactivated virus-containing fluid can be concentrated and blended with an adjuvant and / or antigens directed against one or more other porcine pathogens.

[0038] The method of preparing the composition of the present invention may further comprise mixing the conjugate of one or more SVA peptides or inactivated whole virus preparations and a carrier molecule with a physiologically acceptable vehicle, such as a pharmaceutically or veterinarily acceptable carrier, adjuvant, or combination thereof. Those skilled in the art will recognize that the choice of vehicle, adjuvant, or combination will be determined, among other things, by the route of delivery, personal preference, and animal species.

[0039] In another aspect, the present invention provides a method for diagnosing an SVA infection in a subject. The method comprises providing one or more SVA peptides; contacting the one or more SVA peptides with a sample obtained from the subject; and identifying the subject as having an SVA infection if antibodies capable of binding to the one or more SVA peptides are detected in the sample.

[0040] In another aspect, the present invention provides a method for determining whether a subject has been previously exposed to an SVA infection and is capable of expressing an immune response against SVA. The method comprises providing one or more SVA peptides; contacting the one or more SVA peptides with a sample obtained from the subject; and if antibodies capable of binding to the one or more SVA peptides are detected in the sample, identifying the subject as having an SVA infection.

[0041] The present invention also provides a kit comprising: an immunogenic composition comprising one or more SVA peptides, preferably together with a carrier molecule; a container for packaging the immunogenic composition; a set of printed instructions; and a dispenser capable of administering the immunogenic composition to an animal. Optionally, the one or more SVA peptides and the carrier molecule can be packaged as a conjugate or separate compounds. If supplied separately, means for conjugating the one or more SVA peptides and the carrier molecule are also provided, along with appropriate printed instructions.

[0042] The present invention also provides a kit for vaccinating an animal, comprising a set of printed instructions; a dispenser capable of administering to an animal an immunogenic composition provided herein comprising one or more SVA peptides; and wherein the at least one SVA peptide is effective in immunizing the animal against at least one disease associated with SVA infection. Preferably, the one or more SVA peptides are selected from those provided herein. The kit of the present invention may further comprise a veterinarily acceptable carrier, adjuvant, or a combination thereof.

[0043] The dispenser in the kit of the present invention is capable of dispensing its contents as droplets; and the immunogenic composition comprising an SVA peptide as provided herein, included in the kit, is capable of reducing the severity of at least one clinical sign of SVA infection when administered intranasally, orally, intradermally, or intramuscularly to an animal. Preferably, the severity of the clinical sign is reduced by at least 10%, preferably at least 20%, even more preferably at least 30%, even more preferably at least 50%, even more preferably at least 70%, and most preferably at least 100%, compared to an untreated infected animal.

[0044] Also disclosed are methods for treating or preventing infection caused by SVA. The methods comprise administering to a subject an effective amount of an immunogenic composition of the invention, wherein the treating or preventing is selected from the group consisting of reducing signs of SVA infection, reducing the severity or incidence of clinical signs of SVA infection, reducing the mortality rate of a subject due to SVA infection, and combinations thereof.

[0045] The compositions of the present invention further comprise a veterinarily acceptable carrier, adjuvant, or combination thereof. Such compositions can be used as vaccines, and include inactivated vaccines. Such vaccines elicit a protective immune response against at least one disease associated with SVA.

[0046] Those skilled in the art will appreciate that the compositions used herein can be incorporated into known injectable, physiologically acceptable sterile solutions. In order to prepare ready-to-use solutions for parenteral injection or infusion, isotonic aqueous solutions, such as saline or plasma protein solutions, are readily available. In addition, the immunogenic and vaccine compositions of the present invention may include pharmaceutically or veterinarily acceptable carriers, diluents, isotonic agents, stabilizers, or adjuvants.

[0047] The method of the present invention may further comprise mixing the composition of the present invention with a veterinary acceptable carrier, adjuvant or combination thereof. Those skilled in the art will recognize that the choice of carrier, adjuvant or combination is determined by, among other things, the route of delivery, personal preference and animal species.

[0048] Also disclosed are methods for treating or preventing infection caused by SVA. The methods comprise administering to an animal an effective amount of an immunogenic composition of the present invention, wherein the treatment or prevention is selected from the group consisting of reducing signs of SVA infection, reducing the severity or incidence of clinical signs of SVA infection, reducing the mortality of an animal due to SVA infection, and combinations thereof.

[0049] Preferred routes of administration include intranasal, oral, intradermal and intramuscular. Administration via the intramuscular route is preferred, most preferably in a single dose. Those skilled in the art will recognize that the compositions of the present invention can also be administered in two or more doses, as well as by other routes of administration. For example, such other routes include subcutaneous, intradermal, intravenous, intravascular, intraarterial, intraperitoneal, intrathecal, intratracheal, intradermal, intracardial, intralobar, intramedullary, intrapulmonary or intravaginal. Depending on the required duration and effectiveness for the treatment, the compositions of the present invention can be administered once or several times, and intermittently, for example, on a daily basis for a total of several days, weeks or months, and with different dosages.

[0050] The present invention also provides a kit for vaccinating an animal, comprising a set of printed instructions; a dispenser capable of administering the vaccine to the animal; and at least one isolate from a SVA culture. The kit of the present invention may further comprise a veterinary acceptable carrier, adjuvant, or a combination thereof.

[0051] The dispenser in the kits of the present invention is capable of dispensing its contents as liquid droplets; and the isolate included in the kit is capable of reducing the severity of at least one clinical sign of SVA infection when administered intranasally, orally, intradermally, or intramuscularly to an animal. In some kits, the isolate is also capable of reducing the severity of at least one clinical sign of SVA infection. Preferably, the severity of the clinical sign is reduced by at least 10% compared to an untreated infected animal.

[0052] Other objects, features and advantages of the present invention will become apparent from the following detailed description. However, it should be understood that the detailed description and specific examples, while indicating preferred embodiments of the present invention, are given by way of illustration only, as various changes and modifications within the spirit and scope of the present invention will become apparent to those skilled in the art from this detailed description. Sequence Listing <110> Boehringer Ingelheim Animal Health USA, Inc. <120> Seneca virus A immunogenic compositions and methods thereof <130> 10-0177-WO-1 <160> 45 <170> PatentIn version 3.5 <210> 1 <211> 7221 <212> RNA <213> Seneca virus <400> 1 cuguguuugc uagaggcaca gaggagcaac auccaaccug cuuuuguggg gaacggugcg 60 gcuccaauuc cugcgucgcc aaagguguua gcgcacccaa acggcgcauc uaccaaugcu 120 auuggugugg ucugcgaguu cuagccuacu cguuucuccc cuacucacuc auucacgcac 180 aaaaacugug uuguaacuau aagauuuagc ccucgcacgg gaugugcgau aaccgcaaga 240 uugacucaag cgcggaaagc gcuguaacca caugcuguua gucccuuuau ggcugcgaga 300 uggcuaucca ccucggauca cugaacugga gcucgacccu ccuuaguaag ggaaccgaga 360 ggccuucuug caacaagcuc cgacacagag uccacgugau ugcuaccacc augaguacau 420 gguucucccc ucucgaccca ggacuucuuu uugaauaucc acggcucgau ccagagggug 480 gggcaugauc ccccuagcau agcgagcuac agcgggaacu guagcuaggc cuuagcgugc 540 cuuggauacu gccugauagg gcgacggccu agucgugucg guucuauagg uagcacauac 600 aaguaugcag aacucucauu uuucuuucga uacagccucu ggcaccuuug 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cuggugcccc agcaugcuuc ccuuucgcag cuacaagcag aagaugcuga ugcaaucagg 3420 cgacgucgag accaacccug gcccugcuuc ugacaacccg aucuuggagu uucuugaagc 3480 ggaaaacgau guagucacuc ugggccucucu cuggaaug guacacucug uucaacacuc 3540 3600. 3600. 3600. 3600. 3600. 3600. 3600. 3600. 3600. 3600 ggaaggcucc aucgccuugg ccgccacgcu aucuaaccaa gcuucaguga aagcucucuu gggccugcau uuucucucuc gagggcucau uuacacagau uuuacucuu uacugauaga gaaaugcucu aguuucuuua cuguagaacc gccuccucca ccagcugaa aucugaugac caagcccucc gugaagucga aauuccgaaa gcuguuuaag augcaaggac ccauggacac agucaaagac covered uagccgccgg covered uuccaauuug uucgugaccu agucaaagag gugguugacu ggcuccaggc cuggaucaau aaagagaag ccagcccugu 4020. ccuccaguac cagcuggaga ugaagaagcu cggggcccgug gcuuuggcuc augaugccuu cauggccggu uccgggcccc cucumber cgaccagauu gaauaccucc agaaccucaa aucucuugcc cuaacacugg gaaagacuaa uuuggcccaa aucucacca cuaugaucaa cgccaagcag agcuccgccc aacgagucga acccguugug gugguccuca gaggcaagcc gggaugcggc aaaagcuugg ccuccacguu gauugcccag gcugugucua agcgucucua cggcucacag aguguguauu cucuuccucc ggacccagac uucuucgacg gauauaaagg 4320 acaguuugua accuugaugg acgaucuggg acaaaacccg gaugggcaag auuucuccac 4380 cuuuugucag auggugucga ccgcccaauu ucuucccaac auggcggacc uugcagagaa 4440 ggggcguccc uucaccucca aucuuaucau ugcaacuaca aaccucccuc acuuuagccc 4500 ugucaccauu gcugacccuu cugcagucuc ucggcguauc aacuacgacc ugacucuaga 4560 aguaucugag gccuacaaga agcacacgcg gcugaauuuc gaccuggcuu ucagacgcac 4620 ugacgccccc cccauuuauc cuuuugcugc ccaugugccc uucguggacg uggcugugcg 4680 cuucaaaaau ggucaucaaa gcuucaaucu ccuggaguug gucgacucua uuugugcaga 4740 cauucgggcc aagcaacaag gugcccgaaa uaugcagacu cugguucuac agagcccuaa 4800 cgagaacgac gacacccccg ucgacgaggc guuggguaga guucucaccc ccgcugcggu 4860 cgacgaggcg cuugucgacc ucgcuccaga ugccgacccg guuggccgcu uagcuauucu 4920 cgccaagcua ggucuugccc uagcugcggu cacuccuggu uugauaaucu uggcaguggg 4980 acucuacaag uacuucucug gcucugauac 5040. acucuacaag uacuucucug gcucugauac ugcuaaagug ccuaggagcg agaaugcuua ugacggccca aagaaaaacu ccaagccccc 5160. uggagcgcuc ucucuuaugg aaaugcaaca gcccaacgug gacaugggcu uugaggcugc cover aaaguggucg uccccauuac cover cccaacagac cover uacacagucc gcucuucuug uggccggccg gaccuuccua aucaaugagc auacaugguc caaccccucc uggaccagcu ucacaauccg uggugaggug cacacucgug augagcccuu 5400. ccaacgguu cauuuuacuc accauggucu ucccacagau cugaugaugg uacgucucgg accgggcaac ucuuucccua acaaucuaga uaaguuugga cuugaccaga ugccggcacg uaacucccgu gugguugggcg uuucggcuag uuacgguaac uucucucucucucucucuu ccucggguuu guugacucca ucaccucuga ccaaggaacc uaugcgagac uuuucaggua 5640. 5640. 5640. 5640. 5640. 5640. 5640. 5640. 5640. 5640. 5640. 5640 ccgacgcauc auuggcaugc auucugcugg ugccgcuggu aucggcgccg ggacuuacau cucaaaauua ggacugauca aagcccuuaa acaccucggu gagccucugg cuacaaugca 5760 aggacugaug acugagcuag agccuggagu caccguacau guaccccgaa aaucuaaauu 5820 gagaaagacg accgcacacg cgguguacaa accggaguuu gaaccugcug uguugucaaa 5880 auuugauccc agacugaaca aggauguuga ccuagaugag guaauuuggu cuaaacacac 5940 cgccaacguc ccuuaucaac cuccuuuguu cuacacauac augucagagu acgcucaucg 6000 gguuuucucc uuuuugggaa aagacaauga cauucugacc gucaaagaag caauccuggg 6060 caucccugga cuagacccua uggaucccca cacagcuccg gguuugcccu acgccauuag 6120 cggccuucga cguacugauc ucgucgauuu ugcgaacggc acgguagacc cggcacuggc 6180 caugcagauc cagaaauucu uagacgguga cuacucugau caugucuucc aaacuuuucu 6240 gaaagaugaa aucagacccu cagagaaggu ccgggcggga aaaacccgca uugucgaugu 6300 gccccuccug gcgcacugca uuuggggcag aaugcugcuu gggcgcuuug ccgccaaguu 6360 ucaaucccau ccuggcuuuc uccuuggcuc cgcuaucggg ucugacccug augucuucug 6420 gaccgucaua ggggcucagc ucgagggaag aaagaacacg uaugacgugg acuacagugc 6480 cuuugacucu ucacacggca cuggcuccuu cgaggcucuc aucucucacu uuuucaccgu 6540 ggacaauggu uucagcccug cgcugggacc guaucucaga ucccuggcug ucucggugca 6600 6660 cagccugcug aacacagugc ucaacaaugu gaucaucagg acugcucugg cauugaccua 6720 caaggaauuu gaauugaca ugguugauau caucgccuac ggugacgacc uucugguugg 6780 uacggauuau gaucuggacu ucaugaggu ggcgcggcgc gcugccaaac ugggguauaa 6840 6900 uuuucuaaaa cgcaaauucg uccaaaacaa ugacggcuua uauaaaccag uuauggauuu 6960 aaagaauuug gaagccaugc ucuccuacuu caaaccagga acacuacucg agaagcugca 7020 aucuguuucu auguuggcuc aacauucugg aaaagaagaa uacgauagau ugaugcaccc 7080 cuucgcugac uauggugccg uaccgaguca cguaccug caggcaagau ggagggccuu 7140 guucgacuga ccuggauagc cuaacgcgcu ucggugcugc cggcgauucu gggagaaccc 7200 agucggaaca gaaaagggga a 7221 <210> 2 <211> 6546 <212> RNA <213> Seneca virus <400> 2 augcagaacu cucauuuuuc uuucgauaca gccucuggca ccuuugaaga cguaaccgga 60 acaaaaguca agaucguuga acaccccaga ucggugaaca augguguuua cgauucgucc 120 acucauuuag agauacugaa ccuacagggu gaaauugaaa uuuuaaaguc uuucaacgaa 180 uaccaaauuc gcgccgccaa acaacaacuu ggacuggaca ucguauacga acuacagggu 240 aauguucaga caaccucaaa gaaugauuuu gauucccgcg gcaauaaugg uaacaugacc 300 uucaauuacu acgcaaacac uuaccagaau ucaguagacu ucucgaccuc cucgucggcg 360 ucaggcgccg gacccgggaa cucccggggc ggauuagcgg gucuccucac aaauuucagu 420 ggaaucuuga acccucuugg cuaccucaaa gaucacaaua ccgaagaaau ggaaaacucu 480 gccgaucgag ucauaacgca aacggcgggc aacacugcca uaaacacgca aucaucacug 540 gguguguugu gugccuacgu ugaagacccg accaaaucug acccuccguc cagcagcaca 600 660 uggacaaaag cuguaaaaac cuucucuuuu caggccguuc cgcucccugg agccuuccug 720 ucuagacagg gaggccucaa cggaggggcc uucacggcca cccuacaauag acauuucuua 780 augaagugcg gguggcaggu ccagguucaa ugcaauuuga cgcaauucca ccaaggugcu 840 cuucuuguug ccaugguccc cgaaaccacc cuugauguca aaccugacgg caaggcaaag 900 agcuuacagg agcugaauga agagcagugg guggaaugu cugacgacua ccggaccggg 960 aaaaacaugc cuuuucaguc ucuuggcaca uacuaucggc ccccuaacug gacuuggggc 1020 cccaauuuua uuaaccccua ucaaguaaca gucuucccac accaaauucu gaacgcgaga 1080 1140 gagacacaga acuccuggac ccuccuuguu auggugcuug ucccccugga cuacaaaggag 1200 ggagccacaa cugacccaga aauuacauuu ucuguaaggc cuacaagucc cuacuucaau 1260 gggcuucgua accguuucaa gaccgggacg gacgaggaac aggggcccau ucccacagca 1320 1380 gggaaugugc guaccccucc cgucaauuac cuccccggug aaauaaccga ccucuuacaa 1440 cuggcccgua uacccacucu cauggcguuu gggcgggcgu cugaacccga gccgccuca 1500 gacgcauaug ugcccuacgu ugccguuccu gcccaguucg acgacaagcc ucucaucucc 1560 uucccgauca cccuuucaga uccugucuac cagaacaccc ugguaggcgc caucaguucg 1620 1680 gcaagaggga aauuccugcu cucguauucu cccccaaaaug gagcacaacc acagacccuu 1740 1800 uuugucaucc ccuacaucuc gcccagugau uaccgugaaa cucgggcuau uaccaacuca 1860 guuuauucug cugaugguug guuuagcuug cacaagcuga ccaaaauuac ucuaccaccu 1920 gacugcccac agagucccug uauucucuuu uucgccucug cuggugagga uaacacccuc 1980 cgucucccug uugauuguaa uccuuccuac guguuccacu ccaccgacaa cgccgagacu 2040 gggguuauug aggcagguaa cacugacacc gauuuucucug gugaacuggc ggcuccuggc 2100 2160 guacuggaga aggacgccgu cuucccccgu ccuuuccccca cagcaacagg ugcacagcag 2220 2280 cguuucggcc uguacgucaa cccgucugac aguggcguuc ucgcuaacac uucacuggau 2340 uucaauuuuu acaguuuggc cuguuucacu uacuuuagau cagaccuuga agucacggug 2400 gucucacugg agccagauuu ggaguucgcc guggggugu uccccucugg cagugaguac 2460 2520 ccccgcgcuu ucaccagcaa ggguggaaag guaucuuucg ugcucccuug gaacucuguc 2580 ucuuccgugc uucccgugcg cuggggggc gccuccaagc uuucuucugc cacgcgggggu 2640 cugccggcuc augcugacug ggggaccauu uacgccuuua ucccccgucc caacgagaag 2700 aaaagcaccg cuguaaagca cguggcggug uacguucggu acaagaacgc gcgugccugg 2760 ugcccagca ugcucccuu ucgcagcuac your area ugcugaugca aucaggcgac 2820 gucgagacca acccuggccc ugcuucugac aacccgaucu uggaguuucu ugaagcggaa 2880 2940 agaaaguaug ugagaaacga caauuuuuugg cccaacuugc ucagugagcu agugggggaa 3000 ggcuccaucg ccuuggccgc cacgcuaucu aaccaagcuu cagugaaagc ucucuuggc 3060 3120 3180 cccuccguga agucgaaauu ccgaaagcug uuuaagaugc aaggacccau ggacacaguc 3240 aaagacugga accaaauagc cgccggcuug aagaauuucc aauuuguucg ugaccuaguc 3300 aaagaggugg uugacuggcu ccaggccugg aucaauaaag agaaagccag cccuguccuc 3360 caguaccagc uggagaugaa gaagcucgggg cccguggcuu uggcucauga ugccuucaug 3420 gccgguuccg ggcccccucu uggugacgac cagauugaau accuccagaa ccucaaaucu 3480 cuugcccuaa cacugggaaa gacuaauuug gcccaaaguc ucaccacuau gaucaacgcc 3540 aagcagagcu ccgcccaacg agucgaaccc guuguggugg uccucagagg caagccggga 3600 3660 3720 uuuguaaccu ugauggaacga ucugggacaa aacccggaug ggcaagauuu cuccaccuuu 3780 ugucagaugg ugucgaccgc ccaauuucuu cccaacaugg cggaccuugc agaaaggggg 3840 3900 3960 ucugaggccu acaagaagca cacgcggcug aauuucgacc uggcuuucag acgcacugac 4020 gcccccccca uuuauccuuu ugcugcccau gugcccuucg uggacguggc ugugcgcuuc 4080 aaaaaugguc aucaaagcuu caaucuccug gaguuggucg acucuauuug ugcagacauu 4140 cgggccaagc aacaaggugc ccgaaaoug cagacucugg uucuacagag cccuaacgag 4200 aacgacgaca cccccgucga cgaggcguug gguagaguuc ucacccccgc ugcggucgac 4260 gaggcgcuug ucgaccucgc uccagaugcc gacccgguug gccgcuuagc uauucucgcc 4320 aagcuagguc uugcccuagc ugcggucacu ccugguuuga uaaucuuggc agugggacuc 4380 uacaaguacu ucucuggcuc uguaacagac caagagaaa cagaaaguga ggagccugcu 4440 aaagugccua ggagcgagaa ugcuuaugac ggcccaaaga aaaacuccaa gccccugga 4500 gcgcucucuc uuauggaaau gcaacagccc aacguggaca ugggcuuuga ggcugcaguu 4560 gcuaagaaag uggucguccc cauuaccuuc augguuccca acagaccuuc cggacuuaca 4620 caguccgcuc uucuuguggc cggccggacc uuccuaauca augaccauac augguccaac 4680 cccuccugga ccagcuucac aauccguggu gaggugcaca cucgugauga gcccuuccaa 4740 acgguucauu uuacucacca uggucuuccc acagaucuga ugaugguacg ucucggaccg 4800 ggcaacucuu ucccuaacaa ucuagauaag uuuggacuug accagaugcc ggcacguaac 4860 ucccgugugg uggcguuuc ggcuaguuac gguaacuucu ucucucugg gaacuccuc 4920 ggguuuguug cucucaucac cucugaccaa cucugaccaaug cgagacuuuu cagguacagg 4980 gugacgacuu acaagggaug gugcgguucg gcccuggucu gugaggccgg ugguguccga 5040 cgcaucauug gcaugcauuc ugcuggugcc gcugguaucg gcgccgggac uacaucuca 5100 aaauuaggac ugaucaagc ccuuaaacac cucggugagc cucuggcuac auugcaagga 5160 cugaugacug agcuagagcc uggagucacc guacauguac cccgaaaauc uaaauugaga 5220 aagacgaccg cacacgcggu guacaaccg gaguuugaac cugcuguguu write 5280 gaucccagac ugacaagga ugugaccua gaugagguaa uuuggucuaa accaccgcc 5340 aacgucccuu aucaccucc uuuguucuac acauacuu cagaguacgc ucaucggguu 5400 uucuccuuuu ugggaaaga caugacauu cugaccguca aagaagcaau cuggggcauc 5460 ccuggacuag acccuaugga cuccacaca gcuccggguu ugccuacgc cauagcggc 5520 cucgacgua cugaucucgu cgauuuugcg aacggcacgg uagacccggc acuggccaug 5580 cagauccaga aauucuuaga cggugacuac ucugaucaug ucuuccaaac uuuucugaaa 5640 gaugaaauca gacccucaga gaagguccgg gcgggaaaaa cccgcauugu cgaugugccc 5700 ucccuggcgc acugcauugu gggcagaaug cugcuuggc gcuuugccgc caaguucaa 5760 ucccauccug gcuuucuccu uggcuccgcu aucggguucug acccugaugu cuucuggacc 5820 gucauagggg cucagcucga gggaaaag aacacguaug acguggacua cagugccuuu 5880 5940 aaugguuuca gcccugcgcu gggaccguau cucagauccc uggcugucuc ggugcacgcu 6000 uacggcgagc gucgcaucaa gauuaccgga ggccuccccu cugguugugc cgcgaccagc 6060 cugcugaaca cagugcucaa caaugugauc aucaggacug cucuggcauu gaccuacaag 6120 gaauugaau augacauggu ugauaucauc gccuacggug agccuucu gguuggguacg 6180 gauuaugauc uggacuucaa ugagguggcg cggcgcgcug ccaaacuggg guauaagaug 6240 acuccugcca acaaggguuc ugucuuccu ccgacuuccu cucucuccga ugcuguuuuu 6300 cuaaaacgca aauucgucca aaacaaugac ggcuuauaua aaccaguuau ggauuuaaag 6360 aauuuggaag ccaugcucuc cuacuucaaa ccaggaacac uacucgagaa gcugcaaucu 6420 guuucuaugu uggcucaaca uucuggaaaa gaagaauacg auagauugau gcaccccuuc 6480 gcugacuaug gugccguacc gagucacgag uaccugcagg caagauggag ggccuuguuc 6540 gacuga 6546 <210> 3 <211> 2181 <212> PRT <213> Seneca virus <400> 3 Met Gln Asn Ser His Phe Ser Phe Asp Thr Ala Ser Gly Thr Phe Glu 1 5 10 15 Asp Val Thr Gly Thr Lys Val Lys Ile Val Glu His Pro Arg Ser Val 20 25 30 Asn Asn Gly Val Tyr Asp Ser Ser Thr His Leu Glu Ile Leu Asn Leu 35 40 45 Gln Gly Glu Ile Glu Ile Leu Lys Ser Phe Asn Glu Tyr Gln Ile Arg 50 55 60 Ala Ala Lys Gln Gln Leu Gly Leu Asp Ile Val Tyr Glu Leu Gln Gly 65 70 75 80 Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly Asn Asn 85 90 95 Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn Ser Val 100 105 110 Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly Asn Ser 115 120 125 Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile Leu Asn 130 135 140 Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu Asn Ser 145 150 155 160 Ala Asp Arg Val Ile Thr Gln Thr Ala Gly Asn Thr Ala Ile Asn Thr 165 170 175 Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro Thr Lys 180 185 190 Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr Phe Thr 195 200 205 Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr Lys Ala 210 215 220 Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala Phe Leu 225 230 235 240 Ser Arg Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr Leu His 245 250 255 Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln Cys Asn 260 265 270 Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val Pro Glu 275 280 285 Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu Gln Glu 290 295 300 Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg Thr Gly 305 310 315 320 Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro Pro Asn 325 330 335 Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr Val Phe 340 345 350 Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp Ile Ser 355 360 365 Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr Gln Asn 370 375 380 Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr Lys Glu 385 390 395 400 Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro Thr Ser 405 410 415 Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Lys Thr Gly Thr Asp Glu 420 425 430 Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu Met Phe 435 440 445 Leu Ser Thr Ile Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn Val Arg 450 455 460 Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu Leu Gln 465 470 475 480 Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Gly Arg Ala Ser Glu Pro 485 490 495 Glu Pro Ala Ser Asp Ala Tyr Val Pro Tyr Val Ala Val Pro Ala Gln 500 505 510 Phe Asp Asp Lys Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser Asp Pro 515 520 525 Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe Ala Asn 530 535 540 Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro Met Met 545 550 555 560 Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly Ala Gln 565 570 575 Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile Trp Asp 580 585 590 Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Ile Pro Tyr Ile Ser Pro 595 600 605 Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr Ser Ala 610 615 620 Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu Pro Pro 625 630 635 640 Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala Gly Glu 645 650 655 Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr Val Phe 660 665 670 His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly Asn Thr 675 680 685 Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn His Thr 690 695 700 Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val Ile Lys 705 710 715 720 Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr Ala Thr 725 730 735 Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro Arg Pro 740 745 750 Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Val Asn Pro 755 760 765 Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn Phe Tyr 770 775 780 Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val Thr Val 785 790 795 800 Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe Pro Ser 805 810 815 Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu His Val 820 825 830 Pro Tyr His Phe Thr Gly Arg Thr Pro Arg Ala Phe Thr Ser Lys Gly 835 840 845 Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser Val Leu 850 855 860 Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr Arg Gly 865 870 875 880 Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Ile Pro Arg 885 890 895 Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val Tyr Val 900 905 910 Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro Phe Arg 915 920 925 Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Val Glu Thr Asn 930 935 940 Pro Gly Pro Ala Ser Asp Asn Pro Ile Leu Glu Phe Leu Glu Ala Glu 945 950 955 960 Asn Asp Leu Val Thr Leu Ala Ser Leu Trp Lys Met Val His Ser Val 965 970 975 Gln Gln Thr Trp Arg Lys Tyr Val Lys Asn Asp Asn Phe Trp Pro Asn 980 985 990 Leu Leu Ser Glu Leu Val Gly Glu Gly Ser Ile Ala Leu Ala Ala Thr 995 1000 1005 Leu Ser Asn Gln Ala Ser Val Lys Ala Leu Leu Gly Leu His Phe 1010 1015 1020 Leu Ser Arg Gly Leu Asn Tyr Thr Asp Phe Tyr Ser Leu Leu Ile 1025 1030 1035 Glu Lys Cys Ser Ser Phe Phe Thr Val Glu Pro Pro Pro Pro Pro 1040 1045 1050 Ala Glu Asn Leu Met Thr Lys Pro Ser Val Lys Ser Lys Phe Arg 1055 1060 1065 Lys Leu Phe Lys Met Gln Gly Pro Met Asp Thr Val Lys Asp Trp 1070 1075 1080 Asn Gln Ile Ala Ala Gly Leu Lys Asn Phe Gln Phe Val Arg Asp 1085 1090 1095 Leu Val Lys Glu Val Val Asp Trp Leu Gln Ala Trp Ile Asn Lys 1100 1105 1110 Glu Lys Ala Ser Pro Val Leu Gln Tyr Gln Leu Glu Met Lys Lys 1115 1120 1125 Leu Gly Pro Val Ala Leu Ala His Asp Ala Phe Met Ala Gly Ser 1130 1135 1140 Gly Pro Pro Leu Gly Asp Asp Gln Ile Glu Tyr Leu Gln Asn Leu 1145 1150 1155 Lys Ser Leu Ala Leu Thr Leu Gly Lys Thr Asn Leu Ala Gln Ser 1160 1165 1170 Leu Thr Thr Met Ile Asn Ala Lys Gln Ser Ser Ala Gln Arg Val 1175 1180 1185 Glu Pro Val Val Val Val Leu Arg Gly Lys Pro Gly Cys Gly Lys 1190 1195 1200 Ser Leu Ala Ser Thr Leu Ile Ala Gln Ala Val Ser Lys Arg Leu 1205 1210 1215 Tyr Gly Ser Gln Ser Val Tyr Ser Leu Pro Pro Asp Pro Asp Phe 1220 1225 1230 Phe Asp Gly Tyr Lys Gly Gln Phe Val Thr Leu Met Asp Asp Leu 1235 1240 1245 Gly Gln Asn Pro Asp Gly Gln Asp Phe Ser Thr Phe Cys Gln Met 1250 1255 1260 Val Ser Thr Ala Gln Phe Leu Pro Asn Met Ala Asp Leu Ala Glu 1265 1270 1275 Lys Gly Arg Pro Phe Thr Ser Asn Leu Ile Ile Ala Thr Thr Asn 1280 1285 1290 Leu Pro His Phe Ser Pro Val Thr Ile Ala Asp Pro Ser Ala Val 1295 1300 1305 Ser Arg Arg Ile Asn Tyr Asp Leu Thr Leu Glu Val Ser Glu Ala 1310 1315 1320 Tyr Lys Lys His Thr Arg Leu Asn Phe Asp Leu Ala Phe Arg Arg 1325 1330 1335 Thr Asp Ala Pro Pro Ile Tyr Pro Phe Ala Ala His Val Pro Phe 1340 1345 1350 Val Asp Val Ala Val Arg Phe Lys Asn Gly His Gln Ser Phe Asn 1355 1360 1365 Leu Leu Glu Leu Val Asp Ser Ile Cys Ala Asp Ile Arg Ala Lys 1370 1375 1380 Gln Gln Gly Ala Arg Asn Met Gln Thr Leu Val Leu Gln Ser Pro 1385 1390 1395 Asn Glu Asn Asp Asp Thr Pro Val Asp Glu Ala Leu Gly Arg Val 1400 1405 1410 Leu Thr Pro Ala Ala Val Asp Glu Ala Leu Val Asp Leu Ala Pro 1415 1420 1425 Asp Ala Asp Pro Val Gly Arg Leu Ala Ile Leu Ala Lys Leu Gly 1430 1435 1440 Leu Ala Leu Ala Ala Val Thr Pro Gly Leu Ile Ile Leu Ala Val 1445 1450 1455 Gly Leu Tyr Lys Tyr Phe Ser Gly Ser Asp Thr Asp Gln Glu Glu 1460 1465 1470 Thr Glu Ser Glu Glu Pro Ala Lys Val Pro Arg Ser Glu Asn Ala 1475 1480 1485 Tyr Asp Gly Pro Lys Lys Asn Ser Lys Pro Pro Gly Ala Leu Ser 1490 1495 1500 Leu Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala 1505 1510 1515 Ala Val Ala Lys Lys Val Val Val Pro Ile Thr Phe Met Val Pro 1520 1525 1530 Asn Arg Pro Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Ala Gly 1535 1540 1545 Arg Thr Phe Leu Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp 1550 1555 1560 Thr Ser Phe Thr Ile Arg Gly Glu Val His Thr Arg Asp Glu Pro 1565 1570 1575 Phe Gln Thr Val His Phe Thr His His Gly Leu Pro Thr Asp Leu 1580 1585 1590 Met Met Val Arg Leu Gly Pro Gly Asn Ser Phe Pro Asn Asn Leu 1595 1600 1605 Asp Lys Phe Gly Leu Asp Gln Met Pro Ala Arg Asn Ser Arg Val 1610 1615 1620 Val Gly Val Ser Ala Ser Tyr Gly Asn Phe Phe Phe Ser Gly Asn 1625 1630 1635 Phe Leu Gly Phe Val Asp Ser Ile Thr Ser Asp Gln Gly Thr Tyr 1640 1645 1650 Ala Arg Leu Phe Arg Tyr Arg Val Thr Thr Tyr Lys Gly Trp Cys 1655 1660 1665 Gly Ser Ala Leu Val Cys Glu Ala Gly Gly Val Arg Arg Ile Ile 1670 1675 1680 Gly Met His Ser Ala Gly Ala Ala Gly Ile Gly Ala Gly Thr Tyr 1685 1690 1695 Ile Ser Lys Leu Gly Leu Ile Lys Ala Leu Lys His Leu Gly Glu 1700 1705 1710 Pro Leu Ala Thr Met Gln Gly Leu Met Thr Glu Leu Glu Pro Gly 1715 1720 1725 Val Thr Val His Val Pro Arg Lys Ser Lys Leu Arg Lys Thr Thr 1730 1735 1740 Ala His Ala Val Tyr Lys Pro Glu Phe Glu Pro Ala Val Leu Ser 1745 1750 1755 Lys Phe Asp Pro Arg Leu Asn Lys Asp Val Asp Leu Asp Glu Val 1760 1765 1770 Ile Trp Ser Lys His Thr Ala Asn Val Pro Tyr Gln Pro Pro Leu 1775 1780 1785 Phe Tyr Thr Tyr Met Ser Glu Tyr Ala His Arg Val Phe Ser Phe 1790 1795 1800 Leu Gly Lys Asp Asn Asp Ile Leu Thr Val Lys Glu Ala Ile Leu 1805 1810 1815 Gly Ile Pro Gly Leu Asp Pro Met Asp Pro His Thr Ala Pro Gly 1820 1825 1830 Leu Pro Tyr Ala Ile Ser Gly Leu Arg Arg Thr Asp Leu Val Asp 1835 1840 1845 Phe Ala Asn Gly Thr Val Asp Pro Ala Leu Ala Met Gln Ile Gln 1850 1855 1860 Lys Phe Leu Asp Gly Asp Tyr Ser Asp His Val Phe Gln Thr Phe 1865 1870 1875 Leu Lys Asp Glu Ile Arg Pro Ser Glu Lys Val Arg Ala Gly Lys 1880 1885 1890 Thr Arg Ile Val Asp Val Pro Ser Leu Ala His Cys Ile Val Gly 1895 1900 1905 Arg Met Leu Leu Gly Arg Phe Ala Ala Lys Phe Gln Ser His Pro 1910 1915 1920 Gly Phe Leu Leu Gly Ser Ala Ile Gly Ser Asp Pro Asp Val Phe 1925 1930 1935 Trp Thr Val Ile Gly Ala Gln Leu Glu Gly Arg Lys Asn Thr Tyr 1940 1945 1950 Asp Val Asp Tyr Ser Ala Phe Asp Ser Ser His Gly Thr Gly Ser 1955 1960 1965 Phe Glu Ala Leu Ile Ser His Phe Phe Thr Val Asp Asn Gly Phe 1970 1975 1980 Ser Pro Ala Leu Gly Pro Tyr Leu Arg Ser Leu Ala Val Ser Val 1985 1990 1995 His Ala Tyr Gly Glu Arg Arg Ile Lys Ile Thr Gly Gly Leu Pro 2000 2005 2010 Ser Gly Cys Ala Ala Thr Ser Leu Leu Asn Thr Val Leu Asn Asn 2015 2020 2025 Val Ile Ile Arg Thr Ala Leu Ala Leu Thr Tyr Lys Glu Phe Glu 2030 2035 2040 Tyr Asp Met Val Asp Ile Ile Ala Tyr Gly Asp Asp Leu Leu Val 2045 2050 2055 Gly Thr Asp Tyr Asp Leu Asp Phe Asn Glu Val Ala Arg Arg Ala 2060 2065 2070 Ala Lys Leu Gly Tyr Lys Met Thr Pro Ala Asn Lys Gly Ser Val 2075 2080 2085 Phe Pro Pro Thr Ser Ser Leu Ser Asp Ala Val Phe Leu Lys Arg 2090 2095 2100 Lys Phe Val Gln Asn Asn Asp Gly Leu Tyr Lys Pro Val Met Asp 2105 2110 2115 Leu Lys Asn Leu Glu Ala Met Leu Ser Tyr Phe Lys Pro Gly Thr 2120 2125 2130 Leu Leu Glu Lys Leu Gln Ser Val Ser Met Leu Ala Gln His Ser 2135 2140 2145 Gly Lys Glu Glu Tyr Asp Arg Leu Met His Pro Phe Ala Asp Tyr 2150 2155 2160 Gly Ala Val Pro Ser His Glu Tyr Leu Gln Ala Arg Trp Arg Ala 2165 2170 2175 Leu Phe Asp 2180 <2^10> 4 <2^11> 7300 <2^12> RNA <2^13> Seneca virus <4^00> 4 uuugaaaugg ggggcugggc ccucaugccc aguccuuccu uuccccuucc gggggguaaa 60 ccggcugugu uugcuagagg cacagaggag caacauccaa ccugcucuug ugggggaacgg 120 ugcggcucca auuccugcgu cgccaaaggu guuagcgcac ccaaacggcg caucuaccaa 180 ugcuauug guggucucgc aguucuagcc uacucguuuc uccccuaucc acuacucac 240 gcacaaaaag uguguuguaa cuacaagacu uagcccucgc acgagaugug cgauuaaccgc 300 aagauugacu caagcgcgga aagcgcugua accacaugcu guuagucccu uuauggcugc 360 gagauggcua uccccucgg aucacugaac uggagcucga cccccuuag uaaggggaacc 420 gagaggccuu cuugcaacaa gcuccgacac agaguccacg ugauugcuac caccaugagu 480 acauggucu ccccucucga cccaggacuu cuuuuuugaau auccacggcu cgauccagag 540 gguggggcau gaucccccua gcauagcgag cuaagcggg aacuguagcu aggccuuagc 600 gugcuuugga uacugccuga uagggcgacg gccuagucgu gucgguucua uagguagcac 660 auacaauau cgagaacucu cauuuuucuu ucgauacagc cucuggcacc uuugaagacg 720 uaaccggaac aaaagucaag aucguugaau acccuagauc ggugaacaau gguguuuacg 780 auucguccac ucauuuagag aucugaacc uacaggguga aauugaaauu uuaaggucuu 840 ucaaugaaua ccaaauucgc gccgccaaac aacaacuugg acuggacauc guauaugaac 900 uaacaggguaa uguucaagaca accuacaaga augauuuga uucccgcggc aauaauggua 960 acaugaccuu caauuacuac gcaaacacuu aucagaauuc aguagacuuc ucgaccuccu 1020 cgucggcguc aggcgccgga cccgggaacu cccggggcgg auuagcgggu cuccucacaa 1080 auuucagugg aaucuugaac ccucuuggcu accucaaaga ccacaauacc gaagaaugg 1140 aaaacucugc ugaucgaguc auaacacaaa cggcgggcaa cacugccaua aacacgcaau 1200 caucacuggg uguguugugu gccuaccguug aagacccgac caaaucugac ccuccgucca 1260 1320 ucaauucuug gacaaaagcu guaaaaaccu ucucuuuca ggccgucccg cucccuggag 1380 ccuuccuguc uagacaggga ggccucaaug gagggggccuu cacggcuacc cuacauagac 1440 auuucuuaau gaagugcggg uggcaggugc agguccaaug caauuugaca caauuccacc 1500 aaggugcucu ucuuguugcc augguccccg aaaccaccu ugaugucaag cccgacggca 1560 agggaaagag ccuaggag cugauagaag agcagugggu aggaaugucu gacgauuacc 1620 ggaccgggaaaacaugccu uucagucuc uuggcaua cuaucggccc cuacugga 1680 cuggggccc uaauuucauc aaaauucauc aaaaaaacagu uuuccacac aaaaucuga 1740 acgcgagaac cucuaccucg guagacauaa gugucccaua acgggggg cucucucacac 1800 auccucaga gacagaac ccuggacccc uccucguuau ggugcuuguc ccccuggacu 1860 acaaggagggg agccacaacu gacccagaa uacauuuuc cguaggccu acaaguccuu 1920 acucaaugg gcuucguac cgcuacaaga ccgggacgga cgaggaacag gggcccauuc 1980 ccacagcacc cagagaaau ucgcuuauugu ucucucgac caucccugac cagacugucc 2040 cugcuuacgg gaagugcgu accccuccg ucauuaccu uccuggugaa auaaccgacc 2100 ucuuacacu ggcccguaua cccacucuca uggcguuugg gcgggugccu gaaccugaac 2160 cugccucaga cgcuuaugug cccuacguug ccguucccac ccaguucgau ganaagccuc 2220 ucaucuccuu cccgaucacc cuuucagauc cugucuacca gaauacucug guaggcgcca 2280 ucaguucaaa uuucgccaac uaccgggggu guauccaaau cacucugaca uuuuguggac 2340 cuaugauggc aagagggaaa uuccuacucu cguauucucc cccaaaugga acacaaccac 2400 agacccuuuc ugaagccaug cagugcacau auucuauuug ggauauaggc uuaaacuua 2460 guuggaccuu ugucaucccc uacaucucgc ccagugacua ccgugaaacu cgggccauua 2520 ccaauucggu uuauucugcu gauggcuggu uuagccugca caagcugacc aaaauuacuc 2580 uaccaccuga uugcccacag agccccugua uucucuuuuu cgccucugcu ggugaggau 2640 acacccuccg ucuccccguu gauuguaauc cuucuuaugu guuccacucc accgacaacg 2700 ccgagacugg gguuauugag gcggguaaca cugacaccga uuucucuggu gaauuggcgg 2760 cuccuggcuc uaaccacacu aaugucaagu uccuguuuga ccgauucucga uaacugaaug 2820 uaauuaaggu acuggagaag gacgccgucu ucccccgccc uuucccaca gcaacuggua 2880 cacaacagga cgaugguuac uuuugucuuc uaacaccccg cccaacaguc gccucccgac 2940 ccgccacucg uuucggccug uacgucaguc cgucugacag uggcguucuc gccaacacuu 3000 caucuggauuu caauuuuuac agcuuggccu guuucacuua cuuuagauca gaccuugaag 3060 ucacgguggu cucacuggag ccagaucugg aauucgcugu agggugguuc cccucuggca 3120 gugaguacca ggcuuccagc uuugucuacg accaacugca uguacccuac cauuuaacug 3180 ggcgcacucc ccgcgcuuuc gccagcaagg gugggaaggu aucuuucgug cucccuugga 3240 acucugucuc auccgugcuu cccgugcgcu gggggggcgc uuccaagcuu ucuucugcca 3300 cgcggggucu gccggcucau gcugacuggg gcacuauua cgccuuuauc ccccguccca 3360 acgagaagaa aagcaccgcu guaaagcaug uggccgugua cguucgguac aagaacgcgc 3420 gugccuggug ccccagcaug cuucccuuuc gcagcuacaa gcagagaug cugaugcaau 3480 caggcgacgu cgagaccaac ccaggcccug cuucugacaa cccaaucuug gaauuucuug 3540 aagcagaaaa ugaucuaguc acucuggccu cucucuggaa gauggguacac ucuguucaac 3600 agaccuggag aaaguaugug aagaacgaca auuuuuggcc caauuuacuc agugaguuag 3660 ugggggaagg cuccaucgcc uuggccgcca cgcugucuaa ccaagcuuca guaaaagcuc 3720 ucuugggccu gcauuuucuc ucucgagggc ucaauuacac agacuuuuac ucuuuacuga 3780 uagagaaaug cucuaguuuc uuuacuguag aaccgccucc uccaccagcu gaaaaucuga 3840 ugaccaagcc cuccgugaag ucgaaauucc gaaagcuguu uaagaugcaa ggacccaugg 3900 acaaagucaa agacuggaac caaauagccg ccggcuugaa gaauuuucaa uuuguucgug 3960 accuagucaa agaggugguc gacuggcucc aggccugggau caacaaagag aaagccagcc 4020 cuguccucca guaccaguug gagaugaaga agcucgggcc cguggcuuug gcucaugaug 4080 ccuucauggc cgguuccggg cccccucuua gugacgacca gauugaauac cuccaaaacc 4140 ucaaaucucu ugcccuaaca cuggggaaga cuauuuggc ccaaagucuc accacuauga 4200 ucaaugccaa gcaaaguucc gcccaacgag ucgaacccgu uguggugguc cucagaggca 4260 agccgggaug cggcaagagc uuggccucca cguugauugc ccaggcugug uccaagcguc 4320 ucuacggcuc acaaagugug uauucucuuc cuccggaccc agacuucuuc gacggauaca 4380 agggagou uguaccuug auggauu ugggachaaaa cccggauggg aaaaaaccu 4440 ccaccuuuug ucagauggug ucgaccgccc auuuucuucc ciacauggcg gaccuugcag 4500 agaaggggcg squirrel squirrel squirrel squirrel squirrel squirrel squirrel squirrel 4560 gcccugucac cauugcugau ccucugcgg ucucucgucg gaucacuac gacuugacuc 4620 uagaaguauc ugaggccuac aaaaagcaca cacggcugaa uuuugaccug gcuuucaggc 4680 gcacagacgc ccccccauu uauccuuuug cugcccaugu gccuuucgug gacguggcug 4740 ugcgcuuca aaauggccac ucuuuuugug 4800 cagacauucg agccaagca caaggugccc gaauaugca gacucuagu cuacagagcc 4860 quaacgagaa ugaugacacc cccgucgacg aggcgcuggg uagaguucc acuccccgcug 4920 cggucgacga ggcgcuuguc gaccucgcuc aagaggccga uccgguuggc cgcuuggcua 4980 uucuugccaa acuagucuu gcucuagcug cggucacccc cggcuugaua aucuuggcag 5040 ugggacucua uagguacuuc ucuggcucug augcagacca agagaaacg gaaagugagg 5100 aaccugcuaa agcgccuagg agcgagaaug cuuaugacgg cccgaagaaa aacucuaagc 5160 ccccuggagc gcucucuu auggaaugc aacagcccaa cguggacaug ggcuuugagg 5220 cugcggucgc uaagaaagug gucgucccca uuacauucau gguucccaac agaccuucug 5280 gacuuacaca guccgcccuu cuuguggccg gccggaccuu ccuaauuaau gagcauacau 5340 gguccaaccc cuccuggacc aguuuucacaa uccgugguga ggugcacacu cgugaugagc 5400 5460 ucggaccggg caacucuuuc ccuaacaauc uagacaaguu uggacuugac cagaugccgg 5520 cacguaacuc ccguguugguu ggcguuucgg cuaguuacgg uaauuucuuc uucucuggga 5580 auuuccuugg guuuguugac uccaucaccu cugaacaagg aacuuauagca agacuuuuuua 5640 5700 guguccggcg caucauuggc cugcauucug cuggugccgc ugguaucggc gccgggaccu 5760 acaucucaaa auuaggacug aucaaagccc uuaaacaccu cggugaaccu cuggcuacaa 5820 5880 aauugagaaa gacgaccgca cacgcggugu acaaaccgga guuugaaccu gcuguuguugu 5940 caaaauuuga ucccagacug aacaaggaug uugaccuaga ugagguaauu uggucuaaac 6000 6060 aucgggguuuu cuccuuuuug ggaaaagaca augacauucu gaccguuaaa gaagcaaucc 6120 ugggcauccc uggacuagac ccuauggauc cccacacagc uccgggucug cccuacgcca 6180 uuagcggccu ucgacguacu gaucucgucg auuuuugaa cgguacggua gacgcagcac 6240 uggcaugca aauccagaaa uucuuagacg gugacuacuc ugaucauguc uuccaaacuu 6300 uucugaaaga ugaaaucaga cccucagaga agguccgagc gggaaaaacc cgcauugucg 6360 augugcccuc ccuggcacac ugcauugugg gcagaaugcu gcucgggcgu uucgccgcca 6420 aguuuucaauc ccaucccggc uuucuucuug guucugcuau cggguccgac ccugaugucu 6480 ucuggaccgu cauaggggcu cagcucgagg gaaaagaa cacguacgac guggacuaca 6540 gugccuuuga cucuucacac ggcacuggcu ccuucgaggc ucucaucucu cacuuuuca 6600 ccguugacaa ugguuuuagc ccugcgcugg gaccguaucu cagaucccug gcugucucgg 6660 ugcacgcuua cggcgagcgu cgcaucaaga uuaccggagg ccuucccucu gguugugccg 6720 cgaccagccu ucugaucaca gugcucaaca augugaucau caggacugcu cuggcauuga 6780 cuuacaagga auuugaguau gacaugguug auaucaucgc cuacggugac gaccuuuugg 6840 uuggucgga uuugaucug gacuucaaug aaguggcgcg gcgcgcugcc aaacuggggu 6900 auaagaugac uccugccaac aaggguuccg ucuucccucc gacuuccucu cucuccgaug 6960 cuguuuuucu aaaacgcaaa uucguccaaa acaaugacgg cuuauauaaa ccaguuaugg 7020 auuuaaagaa uuuggaagcc augcucuccu acuucaaacc aggaacacua cucgagaagc 7080 ugcaaucugu uucuauguug gcucaacauu cuggaaaaga agaauaugau agauugaugc 7140 gccccuucgc ugacuacggu gccguaccga gucacgagua ccugcaggca agauggaggg 7200 ccuuguucga uugaccuaga uagcccaacg cgcuucggug ccgccggcga uucugggaga 7260 acucagucgg aacagaaaag ggaaaaaaaa aaaaaaaaaa 7300 <210> 5 <211> 7286 <212> RNA <213> Seneca virus <400> 5 uuugaaaugg ggggcugggc ccucaugccc aguccuuccu uuccccuucc gggggguaaa 60 ccggcugugu uugcuagagg cacagaggag caacauccaa ccugcucuug uggggaacgg 120 ugcggcucca auuccugcgu cgccaaaggu guuagcgcac ccaaacggcg caucuaccaa 180 ugcuauuggu guggucugcg aguucuagcc uacucguuuc uccccuaucc acucacucac 240 gcacaaaaag uguguuguaa cuacaagacu uagcccucgc acgagaugug cgauaaccgc 300 aagauugacu caagcgcgga aagcgcugua accacaugcu guuagucccu uuauggcugc 360 gagauggcua uccaccucgg aucacugaac uggagcucga cccuccuuag uaagggaacc 420 gagaggccuu cuugcaacaa gcuccgacac agaguccacg ugauugcuac caccaugagu 480 acaugguucu ccccucucga cccaggacuu cuuuuugaau auccacggcu cgauccagag 540 gguggggcau gaucccccua gcauagcgag cuacagcggg aacuguagcu aggccuuagc 600 gugcuuugga uacugccuga uagggcgacg gccuagucgu gucgguucua uagguagcac 660 auacaauau cgagaacucu cauuuuucuu ucgauacagc cucuggcacc uuugaagacg 720 uaaccggaac aaaagucaag aucguugaau acccuagauc ggugaacaau gguguuuacg 780 auucguccac ucauuuagag aucugaacc uacaggguga aauugaaauu uuaaggucuu 840 ucaaugaaua ccaaauucgc gccgccaaac aacaacuugg acuggacauc guauaugaac 900 uaacaggguaa uguucaagaca accuacaaga augauuuga uucccgcggc aauaauggua 960 acaugaccuu caauuacuac gcaaacacuu aucagaauuc aguagacuuc ucgaccuccu 1020 cgucggcguc aggcgccgga cccgggaacu cccggggcgg auuagcgggu cuccucacaa 1080 auuucagugg aaucuugaac ccucuuggcu accucaaaga ccacaauacc gaagaaugg 1140 aaaacucugc ugaucgaguc auaacacaaa cggcgggcaa cacugccaua aacacgcaau 1200 caucacuggg uguguugugu gccuaccguug aagacccgac caaaucugac ccuccgucca 1260 1320 ucauucuug gaaaagcu guaaaaaccu ucuuuuca ggccgucccg cucccuggag 1380 cucuccaug oagacaggga ggcucaug gaggggcuu cucucuacc cucauagac 1440 auuucuuaau gaagugcgggg uggcaggugc aggguccaaug caauugaca caauccacc 1500 aaggugcucu ucuuguugcc augguccccg aaaccaccu ugaugucaag cccgacggca 1560 agggaaagag ccuaggag cugauagaag agcagugggu aggaaugucu gacgauuacc 1620 ggaccgggaaaacaugccu uucagucuc uuggcaua cuaucggccc cuacugga 1680 cuggggccc uaauuucauc aaaauucauc aaaaaaacagu uuuccacac aaaaucuga 1740 acgcgagaac cucuaccucg guagacauaa gugucccaua acgggggg cucucucacac 1800 auccucaga gacagaac ccuggacccc uccucguuau ggugcuuguc ccccuggacu 1860 acaaggagggg agccacaacu gacccagaa uacauuuuc cguaggccu acaaguccuu 1920 acucaaugg gcuucguac cgcuacacga ccgggacgga cgaggaacag gggcccauuc 1980 ccacagcacc cagagaaau ucgcuuauugu ucucucgac caucccugac cagacugucc 2040 cugcuuacgg gaaugugcgu acccccucccg ucaauuaccu uccuggugaa auaaccgacc 2100 ucuaacacu ggcccguaua cccacucuca uggcguuugg gcgggugccu gaaccugaac 2160 2220 ucaucuccuu cccgaucacc cuuucagauc cugucuacca gaauacucug guaggcgcca 2280 ucaguucaaa uuucgccaac uaccgggggu guauccaaau cacucugaca uuuuguggac 2340 cuaugauggc aagagggaaa uuccuacucu cguauucucc cccaaaugga acacaaccac 2400 agacccuuuc ugaagccaug cagugcacau auucuauuug ggauauaggc uuaaacuua 2460 guuggaccuu ugucaucccc uacaucucgc ccagugacua ccgugaaacu cgggccauua 2520 ccaauucggu uuauucugcu gauggcuggu uuagccugca caagcugacc aaaauuacuc 2580 uaccaccuga uugcccacag agccccugua uucucuuuuu cgccucugcu ggugaggau 2640 acacccuccg ucuccccguu gauuguaauc cuucuuaugu guuccacucc accgacaacg 2700 ccgagacugg gguuauugag gcggguaaca cugacaccga uuucucuggu gaauuggcgg 2760 cuccuggcuc uaaccacacu aaugucaagu uccuguuuga ccgauucucga uaacugaaug 2820 uaauuaaggu acuggagaag gacgccgucu ucccccgccc uuucccaca gcaacuggua 2880 cacaacagga cgaugguuac uuuugucuuc uaacaccccg cccaacaguc gccucccgac 2940 ccgccacucg uuucggccug uacgucaguc cgucugacag uggcguucuc gccaacacuu 3000 caucuggauuu caauuuuuac agcuuggccu guuucacuua cuuuagauca gaccuugaag 3060 ucacgguggu cucacuggag ccagaucugg aauucgcugu agggugguuc cccucuggca 3120 gugaguacca ggcuuccagc uuugucuacg accaacugca uguacccuac cauuuaacug 3180 ggcgcacucc ccgcgcuuuc gccagcaagg gugggaaggu aucuuucgug cucccuugga 3240 acucugucuc auccgugcuu cccgugcgcu gggggggcgc uuccaagcuu ucuucugcca 3300 cgcggggucu gccggcucau gcugacuggg gcacuauua cgccuuuauc ccccguccca 3360 acgagaagaa aagcaccgcu guaaagcaug uggccgugua cguucgguac aagaacgcgc 3420 gugccuggug ccccagcaug cuucccuuuc gcagcuacaa gcagagaug cugaugcaau 3480 caggcgacgu cgagaccaac ccaggcccug cuucugacaa cccaaucuug gaauuucuug 3540 aagcagaaaa ugaucuaguc acucuggccu cucucuggaa gaugguacac ucuguucaac 3600 agaccuggag aaaguaugug aagaacgaca auuuuuggcc caauuuacuc agugaguuag 3660 ugggggaagg cuccaucgcc uuggccgcca cgcugucuaa ccaagcuuca guaaaagcuc 3720 ucuugggccu gcauuuucuc ucucgagggc ucaauuacac agacuuuuac ucuuuacuga 3780 uagagaaaug cucuaguuuc uuuacuguag aaccgccucc uccaccagcu gaaaaucuga 3840 ugaccaagcc cuccgugaag ucgaaauucc gaaagcuguu uaagaugcaa ggacccaugg 3900 acaaagucaa agacuggaac caaauagccg ccggcuugaa gaauuuucaa uuuguucgug 3960 accuagucaa agaggugguc gacuggcucc aggccugggau caacaaagag aaagccagcc 4020 cuguccucca guaccaguug gagaugaaga agcucgggcc cguggcuuug gcucaugaug 4080 ccuucauggc cgguuccggg cccccucuua gugacgacca gauugaauac cuccaaaacc 4140 ucaaaucucu ugcccuaaca cuggggaaga cuauuuggc ccaaagucuc accacuauga 4200 ucaugccaa gcaaguucc gccaacgag ucgaacccgu ugugguc cucagaggca 4260 agccgggaug cggcaagagc uuggccucca cguugauugc ccaggcugug uccaagcguc 4320 ucuacggcuc acaagugug uauucucuc cuccggaccc agacuucuuc gacggauaca 4380 agggagou uguaccuug auggauu ugggachaaaa cccggauggg aaaaaaccu 4440 ccaccuuuug ucagauggug ucgaccgccc auuuucuucc ciacauggcg gaccuugcag 4500 agaaggggcg squirrel squirrel squirrel squirrel squirrel squirrel squirrel squirrel 4560 gcccugucac cauugcugau ccucugcgg ucucucgucg gaucacuac gacuugacuc 4620 uagaaguauc ugaggccuac aaaaagcaca cacggcugaa uuuugaccug gcuuucaggc 4680 gcacagacgc ccccccauu uauccuuuug cugcccaugu gccuuucgug gacguggcug 4740 ugcgcuuca aaauggccac ucuuuuugug 4800 cagacauucg agccaagca caaggugccc gaauaugca gacucuagu cuacagagcc 4860 quaacgagaa ugaugacacc cccgucgacg aggcgcuggg uagaguucc acuccccgcug 4920 cggucgacga ggcgcuuguc gaccucgcuc aagaggccga uccgguuggc cgcuuggcua 4980 uucuugccaa acuaggucuu gcucuagcug cggucacccc cggcuugaua aucuuggcag 5040 ugggacua uagguacuuc ucuggcucug augcagacca aagaaacg gaagugagg 5100 aaccugcuaa agcgccuagg agcgagaaug cuuaugacgg cccgaagaaa aacucuaagc 5160 ccccuggagc gcucucuu auggaaugc aacagcccaa cguggacaug ggcuuugagg 5220 cugcggucgc uaagaaagug gucgucccca uuacauucau gguucccaac agaccuucug 5280 gacuuacaca guccgcccuu cuuguggccg gccggaccuu ccuaauuaau gagcauacau 5340 gguccaaccc cuccuggacc aguuuucacaa uccgugguga ggugcacacu cgugaugagc 5400 5460 ucggaccggg caacucuuuc ccuaacaauc uagacaaguu uggacuugac cagaugccgg 5520 cacguaacuc ccguguugguu ggcguuucgg cuaguuacgg uaauuucuuc uucucuggga 5580 auuuccuugg guuuguugac uccaucaccu cugaacaagg aacuuauagca agacuuuuuua 5640 ggguacagggu gaccaccuac aagggauggu gcgguucggc ccuggucugu gaggccggug 5760. 5760. 5760. 5760. 5760. 5760. 5760. 5760. 5760. 5760 acaucucaaa auuaggacug aucaaagccc uuaaacaccu cggugaaccu cuggcuacac ugcaaggacu gaugacugag cuagagccug gagucaccgu gcauguaccc cggaaucua aauugagaaa gacgaccgca cacgcggugu acaaaccgga guuugaaccu gcuguguugu 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000 6060. acacugccaa cgucccuuau cacuccuu uguucuacac auacauguca gaguacgcuc aucggguuuu cuccuuuuug ggaaaagaca augacauucu gaccguuaaa gaagcaaucc ugggcauccc uggacuagac ccuauggauc cccacacagc uccgggucug cccuacgcca 6240. uuagcggccu ucgacguacu gaucucgucg auuuugugaa cgguacggua gacgcagcac 6300. uggccaugca aauccagaaa uucuuagacg ugaucauguc uuccaaacuu 6360. uucugaaaga ugaaaucaga cccucagaga agguccgagc gggaaaaacc cgcauugucg augugcccuc ccuggcacac ugcauugugg gcagaaugcu gcucgggcgu uucgccgcca 6420 aguuuucaauc ccaucccggc uuucuucuug guucugcuau cggguccgac ccugaugucu 6480 ucuggaccgu cauaggggcu cagcucgagg gaaaagaa cacguacgac guggacuaca 6540 gugccuuuga cucuucacac ggcacuggcu ccuucgaggc ucucaucucu cauuuuuca 6600 ccguugacaa ugguuuuagc ccugcgcugg gaccguaucu cagaucccug gcugucucgg 6660 ugcacgcuua cggcgagcgu cgcaucaaga uuaccggagg ccuucccucu gguugugccg 6720 6780 cuuaacaagga auuugaguau gacaugguug auucaucgc cuacggugac gaccuuuugg 6840 uugguacgga uuaugaucug gacuucaaug aaguggcgcg gcgcgcugcc aaacuggggu 6900 auaagaugac uccugccaac aaggguuccg ucuucccucc gacuuccucu cucuccgaug 6960 cuguuuuucu aaaacgcaaa uucguccaaa acaaugacgg cuuauauaaa ccaguuaugg 7020 auuuaaagaa uuugggaagcc augcucuccu acuucaaacc aggaacacua cucgagaagc 7080 ugcaaucugu uucuauguug gcucaacauu cuggaaaaga agaauaugau agauugaugc 7140 accccuucgc ugacuacggu gccguaccga gucacgagua ccugcaggca agauggaggg 7200 ccuuguucga uugaccuaga uagcccaacg cgcuucggug ccgccggcga uucugggaga 7260 acucagucgg aacagaaaag ggaaaa 7286 <210> 6 <211> 7270 <212> RNA <213> Seneca virus <400> 6 cugggcccuc augcccaguc cuuccuuucc ccuuccgggg gguaaaccgg cuguguuugc 60 uagaggcaca gaggagcaac auccaaccug cuuuuguggg gaacggugcg gcuccaauuc 120[[ID=​​​​​​​​​​caaagcuc cgacacagag uccacgugau ugcuaccacc augaguacau gguucuccccc 480 540 600 gccugauagg gcgacggccu agucgucg guucuauagg uagcacauac aauauugcag 660 aacucucacu uuucuuucga uacagcccu ggcaccuuug aagacguaac cggaacaaaa 720 780 uuagagauac ugaaccuaca gggugaaauu gaauuuuaa agucuuuucaa ugaauaccaa 840 auucgcgccg ccaaacaaca acuuggacug gacaucguau acgaacuaca ggguaauguu 900 cagacaaccu caaagaauga uuuugauucc cgcgguaaua augguaacau gaccuucaau 960 uacuacgcaa acacuuacca gaauucagua gacuucucga ccccucgguc ggcgucaggc 1020 gccggacccg ggaacucccg gggcggauua gcgggucucc ucacaaauuu caguggaauc 1080 uugaacccuc uuggcuaccu caaagaucac aauaccgaag aauggaaaa cucugcugau 1140 cgagucauaa cgcaaacggc gggcaacacu gccauaaaca cgcaaucauc acugggugug 1200 uugugugcccu acguugaaga cccgaccaaa ucugacccuc cguccagcag cacagaucaa 1260 cccaccacca cuuuuaacugc caucgacagg ugguacacug gacgccucaa uucuuggaca 1320 aaagcuguaa aaaccuucuc uuuucaggcc gucccgcucc cuggagccuu ccugucuaga 1380 cagggaggcc ucaacggagg ggccuucacg gcuacccuac auagacauuu cuuaaugaag 1440 ugcggguggc aggugcaggu ccaaugcaau uugacgcaau uccaccaagg ugcucuucuu 1500 guugccaugg uccccgaaac cacccuugau gucaaaccug acggcaaggc aaagagcuua 1560 caggagcuga augaagaaca guggguggaa augucugacg auuaccggac cgggaaaaac 1620 augccuucc agucucuugg cacauacuau cggcccccua acuggacuug gggccccaau 1680 uucaucaacc ccuaucaagu aacaguuuuc ccacaccaaa uucugaacgc gagaaccucu 1740 accucgguag acauaagugu cccauacauc ggggagacuc cuacaacaauc cucagagaca 1800 cagaacuccu ggacccuccu cguuauggug cuugucccccc uggacuacaa ggagggagcc 1860 acaacugacc cagaauuac auuuucugua aggccuaca accumulate caaugggcua cguaaccguu ucacgaccgg gacggacgag gaacaggggc ccauucccac agcacccaga gaaaauucgc uuauguuucu cucaaccauc ccugacgaca cugucccugc uuacgggaau 2100. snow snow snow snow snow snow snow snow snow 2160. cguauaccca cucucauggc guucgggcgg ccgagccugc cucagacaca uauugugcccu acguugccgu uccugcccag uucgacgaca agccucucau cuccuucccg aucacccuuu cagauccugu cuaccagaac acccugguag gcgccaucag uucgaauuuc gccaacuacc ggggguguau gccaaucacu cugacauuuu gccaaccau gauggcaaga gggaaauucc uacucucgua uacuccucca aauggagcac aaccacagac ccuuucuga gcuaugcagu gcacguacuc uauuugggau auaggcuuga acucuaguug gaccuuuguc auccccuaca ucucgcccag ugauuaccgu gaaacucggg cuauuacca cucuguuuauau 2580. ucugcugaug guugguuuag ccugcacaag cugacuaaaa uuacucuacc accugacugc ccacagaguc ccuguauucu cuuuuuucgcc ucugcuggug aggauuacac ccuccgucuc 2640 ccuguugauu guaauccuuc cuacguguuc cacuccaccg acaacgccga gacuggguu 2700 2760 cacacuaaug ucaaguuccu guuugaucga ucucgauuac ugaauguaau uaagguacug 2820 gagaaggacg ccgucuuccc ccguccuuuc cccacagcaa caggugcaca gcaggacgau 2880 gguuacuuuu gccuucuaac accccgccca acagucgcuu cccgacccgc cacucguuuc 2940 ggccuguacg ucaauccguc ugacaguggc guucucgcua acacuucacu ggauuucaau 3000 uuuuacagcu uggccuguuu cacuuacuuu agaucagacc uugaagucac gguggucuca 3060 cggagccag aucuggaauu cgcuguaggg ugguuccccu cuggcaguga guaccaggcu 3120 uccagcuuug ucuacgacca acugcaugua cccuaccacu uuacugggcg cacuccccgc 3180 gcuuucacca gcaagggugg aaagguaucu uucgugcuuc cuuggaacuc ugucucaucc 3240 gugcuucccg ugcgcugggg gggcgcuucc aagcuuucuu ccgccacgcg gggucuaccg 3300 gcuaugcug acugggggac cauuuacgcc uucauccccc guccuaauga gaaaaagc 3360 accgcugcaa agcacguggc gguguacguu cgguacaaga acgcgcgugc cuggugcccc 3420 3480 accaacccug gcccugcuuc ugacaaccca aucuuggagu uucuugaagc ugaaaacgau 3540 cuacuacuc uggcucucu cuggagaaug guacacucug uucaacagac cuggagaaag 3600 uaugugaaga augacaauuu uuggcccaau uugcucagug agcuagugg ggaaggcucc 3660 aucgccuugg ccgccacgcu aucuaaccaa gcuucaguaa aagcucucuu aggccugcau 3720 uuucucuccc gagggcucaa uuauacagau uuuaacucuu uacugauaga gaaaugcucu 3780 aguuucuuua cuguagaacc gccuccucca ccagcugaaa aucugaugac caagcccucc 3840 gugaggucga aauuccgaaa gcuguuuaag augcaaggac ccauggacac agucaaagac 3900 uggaaccaaa uagccgccgg ccugaagaau uuccaauuug uucgugaccu ggucaaagag 3960 guggucgauu ggcuccaggc cuggauuaau aaagagaaag ccagcccugu ccuccaguac 4020 cagcuggaga ugaagaaacu cggacccgug gcuuuggcuc augaugccuu cauggccggu 4080 uccgggcccc cucuuaguga cgaccagauu gaauaucucc agaaccucaa aucucuugcc 4140 cuaacacugg ggaagacuaa uuuggcccaa agucucacca cuaugaucaa ugccaagcaa 4200 agcuccgcuc aacgagucga acccguugug gugguccuua gaggcaagcc gggauguggc 4260 aagagcuugg ccuccacguu gauugcccag gcugugucca agcgucucua cggcucacaa 4320 aguguguauu cucuuccucc ggauccagac uucuucgacg gauacaaagg acaguuugua 4380 accuugaugg acgaucuggg acaaaacccg gaugggcaag auuucuccac cuuuugucag 4440 auggugucga ccgcccaauu ucuucccaac auggcggacc uuacagagaa ggggcguccc 4500 uucaccucca aucuuaucau ugcaacuaca aaccucccuc acuucagccc ugucaccauu 4560 gcugauccuu cugcagucuc ucgucguauc aacuacgacc ugacucuaga aguaucugag 4620 gccuauaaga agcacacacg gcugaauuuu gaccuggcuu ucagacgcac agacgccccc 4680 cccauuuauc cuuuugcugc ccaugugccc uucguggacg uggcugugcg cuucaaaaau 4740 ggucaucaga gcuucaaucu ccuagaguug gucgacucua uuugugcaga cauucgagcc 4800 aagcaacaag gugcccgaaa uaugcagacu cugguucuac agagccccaa cgagaacgac 4860 4920 cuugucgacc ucgcuccaga ugccgaccg guuggccguu uggcuauucu cgccaagcua 4980 ggucuugccc uagcugcggu caccccuggu uugauaaucu uggcaggggg acuucacagg 5040 5100 ccuaggagcg agaaugcuua ugacggcccg aagaaaaacu cuaagcccccc uggagcgcuc 5160 ucccuuaugg aaaugcaaca gcccaacgug gacaugggcu uugaggcugc ggucgcuaag 5220 aaaguggucg uccccauuac cuucaugguu cccaacagac cuucuggacu uacacagucc 5280 gcucuuug uggccggccg gaccuuccua auuaaugagc auacaugguc caaccccucc 5340 uggaccagcu ucacaauccg uggugaggug caacccggug augagccuu ccaaacgguu 5400 cauuuuaacuc accauggugu ucccacagau cuggugaugg uacgucucgg accgggcaac 5460 uccuucccua acaaucuaga caaguuugga cuugaucaga ugccggcacg uaacucccgu 5520 gugguggcg uuucggcuag uuacggcaac uucuucuucu cugggaacuu ccucggguuu 5580 guugacucca ucaccucuga ccaaggaacu uaugcgagac uuuuuaggua cagggugacg 5640 accuacaagg gguggugcgg uucggcccug guuugugagg ccgguggugu ccggcgcauc 5700 auuggccugc auucugcugg ugccgcuggu aucggcgccg ggacuuacau cucaaaauua 5760 ggacugauca aagcccucaa acaccucggu gaaccucugg cuacaaugca aggacugaug 5820 acugagcuag agccuggagu caccgugcau guaccccgga aaucuaaauu gagaaagacg 5880 accgcacacg cgguguacaa accggaguuu gaaccugcug uguugucaaa auuugauccc 5940 agacugaaca aggauguuga ccuagaugag guaauuuggu cuaaacacac ugccaacguc 6000 ccuuaucaac cuccuuuguu cuacacauac augucagagu acgcucaucg gguuuucucc 6060 uuuuuugggaa aagacaauga cauucugacc gucaaagaag caaucuuggg aaucccugga 6120 cuagacccua uggaucccca cacagcuccg ggucugcccu acgccauuag cggcccuucga 6180 6240 caaaaauucu uagacgguga cuacucuguau caugucuucc aaacuuuucu gaagaugaa 6300 aucagacccu cagagaaggu ccgagcggga aaaacccgca uugucgaugu gcccuccug 6360 gcgcacugca uugugggcag aauguugcuu gggcgcuuug ccgccaaguu ucaaucccau 6420 ccuggcuuuc uccuuggcuc ugcuaucggg ucugacccug augucuucug gaccgucaua 6480 ggggcucagc ucgagggaag aaagaacacg uaugacgugg acuacagugc cuuugacucu 6540 ucacacggca cuggcuccuu cgaggcucuc aucucucacu uuuucaccgu ggacaauggu 6600 uuuagcccug cgcugggauc guaucucaga ucccuggcug ucucggugca cgcuuacggc 6660 6720 aacacagugc ucaacaaugu gaucaucagg acugcucugg cauugaccua caaggaauuu 6780 6840 gaucugacu ucaugaggu ggcgcggcgc gcugccaaac ugggguauaa gaugacuccu 6900 gccaacaaag guucugucuu cccuccgacu uccucucucu ccgacgcugu uuuucuaaaa 6960 cgcaaauucg uccaaaacaa ugacggcuua uacaaaccag uuauggauuu aaagaauuug 7020 gaagccaugc ucuccuacuu caaaccagga acacuacucg agaagcugca aucuguuucu 7080<000103" +auguuggcuc aacauucugg aaaagaagaa uaugauagau ugaugcaccc cuuugcugac 7140 uacggugccg uaccgaguca cgaguaccug caggcaagau ggagggccuu guucgacuga 7200<000" +ccuagauagc ccaacgcgcu ccggugcugc cggcgauucu gggagaacuc agucggaaca 7260 gaaaagggaa 7270 <210> " + <211> 7270 <212> RNA <213> Seneca virus <400> " + cugggcccuc augcccaguc cuuccuuucc ccuuccgggg gguaaaccgg cuguguuugc 60 uagaggcaca gaggagcaac auccaaccug cuuuuguggg gaacggugcg gcuccaauuc 120 cugcgucgcc aaagguguua gcgcacccaa acggcgcauc uaccaaugcu auuggugugg 180 ucugcgaguu cuagccuacu cguuucuccc cuauccacuc acucacgcac aaaaagugug 240<" +cuguaauuac aagauuuagc ccucgcacga gaugugcgau aaccgcaaga uugacuaag 300 cgcggaaagc gcuguaacca caugcuguua gucccuucau ggcugcgaga uggcuaucca 360 ccucggauca cugaacugga gcucgacccu ccuuaguaag ggaaccgaga ggccuucuug 420 caaagcuc cgacacagag uccacgugau ugcuaccacc augaguacau gguucuccccc 480 540 600 gccugauagg gcgacggccu agucgucg guucuauagg uagcacauac aauauugcag 660 aacucucacu uuucuuucga uacagcccu ggcaccuuug aagacguaac cggaacaaaa 720 780 uuagagauac ugaaccuaca gggugaaauu gaauuuuaa agucuuuucaa ugaauaccaa 840 auucgcgccg ccaaacaaca acuuggacug gacaucguau acgaacuaca ggguaauguu 900 cagacaaccu caaagaauga uuuugauucc cgcgguaaua augguaacau gaccuucaau 960 uacuacgcaa acacuuacca gaauucagua gacuucucga ccccucguc ggcgucaggc 1020 gccggacccg ggaacucccg gggcggauua gcgggucucc ucacaaauuu caguggaauc 1080 uugaacccuc uuggcuaccu caaagaucac aauaccgaag aaauggaaa cucugcugau 1140 cgagucauaa cgcaaacggc gggcaacacu gccauaaaca cgcaaucauc acugggugug 1200 uugugugcccu acguugaaga cccgaccaaa ucugacccuc cguccagcag cacagaucaa 1260 cccaccacca cuuuuaacugc caucgacagg ugguacacug gacgccucaa uucuuggaca 1320 aaagcuguaa aaaccuucuc uuuucaggcc gucccgcucc cuggagccuu ccugucuaga 1380 cagggaggcc ucaacggagg ggccuucacg gcuacccuac auagacauuu cuuaaugaag 1440 ugcggguggc aggugcaggu ccaaugcaau uugacgcaau uccaccaagg ugcucuucuu 1500 guugccaugg uccccgaaac cacccuugau gucaaaccug acggcaaggc aaagagcuua 1560 caggagcuga augaagaaca guggguggaa augucugacg auuaccggac cgggaaaaac 1620 augccuucc agucucuugg cacauacuau cggcccccua acuggacuug gggccccaau 1680 uucaucaacc ccuaucaagu aacaguuuuc ccacaccaaa uucugaacgc gagaaccucu 1740 1800 cagaacuccu ggacccuccu cguuauggug cuugucccccc uggacuaacaa ggagggagcc 1860 acaacugacc cagaaauuac auuuucugua aggccuaacaa guccuuacuu caaugggcua 1920 cguaaccguu ucacgaccgg gacggacgag gaacaggggc ccauucccac agcacccaga 1980 gaaauucgc uuauguuucu cucaaccauc ccugacgaca cuguccugc uuacgggaau 2040 gugcguaccc cucccgucaa uuaccucccu ggugaaauaa ccgaccucuu acaacuggcc 2100 cguauaccca cucuauggc guucgggcgg gugugugaac ccgagccugc cucagacaca 2160 uaugugcccu acguugccgu uccugcccag uucgacgca agccucucau cuccuucccg 2220 aucacccuuu cagauccugu cuaccagaac acccugguag gcgccaucag uucgaauuuc 2280 gccaacuacc ggggguguau ccaaaucacu cugacauuuuu guggacccau gauggcaaga 2340 gggaaauucc uacucucgua uucuccucca aauggagcac aaccacagac ccuuucugaa 2400 gcuaugcagu gcacguacuc uauuugggau auaggcuuga acucuaguug gaccuuuguc auccccuaca ucucgcccag ugauuaccgu gaaacucggg cuauuacca cucuguuuauau 2580. ucugcugaug guugguuuag ccugcacaag cugacuaaaa uuacucuacc accugacugc 2640. cccagaguc ccuguauucu cuuuuucgcc ucugcuggug aggauuacac ccuccgucuc ccuguugauu guaauccuuc cuacguguuc cacuccaccg acaacgccga gacugggguu auugaggcgg guaacacuga caccgacuuc ucuggugagc uggcggcucc uggcucuaac 2820. 2820. 2820. 2820. 2820. 2820. 2820 gagaaggacg ccgucuuccc ccguccuuuc cccacagcaa caggugcaca gcaggacgau gguaacuuuu gccuucuaac accccgccca acagucgcuu cccgacccgc cacucguuc ggccuguacg ucaauccguc ugacaguggc ggccuguacg acacuucau ggauuucaau uuuuacagcu uggccuguuu cacuuacuuu agaucagacc uugaagucac gguggucuca cuggagccag aucuggaauu cgcuguaggg coverccccu cuggcaguga guaccaggcu uccagcuuug ucuacgacca acugcaugua cccuaccacu uuacugggcg cacuccccgc 3180 gcuuucacca gcaagggugg aaagguaucu uucgugcuuc cuuggaacuc ugucucaucc 3240 gugcuucccg ugcgcugggg gggcgcuucc aagcuuucuu ccgccacgcg gggucuaccg 3300 gcuaugcug acugggggac cauuuacgcc uucauccccc guccuaauga gaaaaagc 3360 accgcugcaa agcacguggc gguguacguu cgguacaaga acgcgcgugc cuggugcccc 3420 3480 accaacccug gcccugcuuc ugacaaccca aucuuggagu uucuugaagc ugaaaacgau 3540 cuacuacuc uggcucucu cuggagaaug guacacucug uucaacagac cuggagaaag 3600 uaugugaaga augacaauuu uuggcccaau uugcucagug agcuagugg ggaaggcucc 3660 aucgccuugg ccgccacgcu aucuaaccaa gcuucaguaa aagcucucuu aggccugcau 3720 uuucucuccc gagggcucaa uuauacagau uuuaacucuu uacugauaga gaaaugcucu 3780 aguuucuuua cuguagaacc gccuccucca ccagcugaaa aucugaugac caagcccucc 3840 gugaggucga aauuccgaaa gcuguuuaag augcaaggac ccauggacac agucaaagac 3900 uggaaccaaa uagccgccgg ccugaagaau uuccaauuug uucgugaccu ggucaaagag 3960 guggucgauu ggcuccaggc cuggauuaau aaagagaaag ccagcccugu ccuccaguac 4020 cagcuggaga ugagaaacu cggacccgug gcuuggcuc augaugccuu cauggccggu 4080 uccgggcccc cucuuaguga cgaccagauu gaauaucucc agaaccucaa aucucuugcc 4140 cuaacugg ggagacuaa uuuggcccaa agucucacca cuaugaucaa ugccaagcaa 4200 4260 aagagcuugg ccuccacguu gauugcccag gcugugucca agcgucucua cggcucacaa 4320 aguguguauu cucuuccucc ggauccagac uucuucgacg gauacaaagg acaguuugua 4380 accuugaugg acgaucuggg acaaaacccg gaugggcaag auucuccac cuuuugucag 4440 auggugucga ccgcccaauu ucuucccaac auggcggacc uuacagagaa ggggcguccc 4500 4560 gcugauccuu cugcagucuc ucgucguauc aacuacgacc ugacucuaga aguaucugag 4620 gccuauaaga agcacacacg gcugaauuuu gaccuggcuu ucagacgcac agacgcccc 4680 cccauuuauc cuuuugcugc ccaugugccc uucguggacg uggcugugcg cuucaaaaau 4740 ggucaucaga gcuucaaucu ccuagaguug gucgacucua uuugugcaga cauucgagcc 4800 aagcaacaag gugcccgaaa uaugcagacu cugguucuac agagccccaa cgagaacgac 4860 gacacccccg ucgacgaggc guuggguaga guucucaccc ccgcugcggu cgacgaggcg 4920 cuugucgacc ucgcuccaga ugccgacccg guuggccguu uggcuauucu cgccaagcua 4980 ggucuugccc uagcugcggu caccccuggu uugauaaucu uggcagugg acucuacagg 5040 uacuucucug gcucugauac agaccaagaa gaaacagaag guggagcc ugcuaaagcg 5100 ccuaggagcg agaaugcuua ugacggcccg aagaaaaacu cuaagcccccc uggagcgcuc 5160 ucccuuaugg aaaugcaaca gcccaacgug gacaugggcu uugaggcugc ggucgcuaag 5220 aaaguggucg uccccauuac cuucaugguu cccaacagac cuucuggacu uacacagucc 5280 gcucuucuug uggccggccg cucumber auuaaugagc auacaugguc caaccccucc 5400. 5400. 5400. 5400. 5400. 5400. 5400. 5400. 5400. cauuuuacuc accauggugu ucccacagau cuggugaugg uacgucucgg accgggcaac 5520. ugccggcacg uaacucccgu snow uuucggcuag uuacggcaac snow snow snow snow snow snow snow snow snow snow snow snow snow snow accuacaagg gguggugcgg uucggcccug guuugugagg ccgguggugu ccggcgcauc auuggccugc auucugcugg ugccgcuggu aucggcgccg ggacuuacau cucaaaauua ggacugauca aagcccucaa acaccucggu gaaccucugg cuacaaugca aggacugaug acugagcuag agccuggagu caccgugcau guaccccgga aaucuaaauu gagaaagacg accgcacacg cgguguacaa accggaguuu gaaccugcug uguugucaaa auuugauccc 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000. 6000 ccuuaucaac cuccuuuguu cuacacauac augucagagu acgcucaucg gguuuucucc 6060 uuuuuugggaa aagacaauga cauucugacc gucaaagaag caaucuuggg aaucccugga 6120 cuagacccua uggaucccca cacagcuccg ggucugcccu acgccauuag cggcccuucga 6180 cguacugauc ucgucgauuu ugcgaacggc acgguggacc cgacacuggc cguacaaauc 6240 caaaaauucu uagacgguga cuacucugau caugucuucc aaacuuuucu gaaagaugaa 6300 aucagacccu cagagaaggu ccgagcggga aaaacccgca uugucgaugu gccccccug 6360 gcgcacugca uugugggcag aauguugcuu gggcgcuuug ccgccaaguu ucaaucccau 6420 ccuggcuuuc uccuuggcuc ugcuaucggg ucugacccug augucuucug gaccgucaua 6480 ggggcucagc ucgagggaag aaagaacacg uaugacgugg acuacagugc cuuugacucu 6540 ucacacggca cuggcuccuu cgaggcucuc aucucucacu uuuucaccgu ggacaauggu 6600 uuuagcccug cgcugggauc guaucucaga ucccuggcug ucucggugca cgcuuacggc 6660 gagcgucgca ucaagauuac cggaggccuc cccucugguu gugccgcgac cagccugcug 6720 aacacagugc ucaacaaugu gaucaucagg acugcucugg cauugaccua caaggaauuu 6780 gaauaugaca ugguugauau caucgccuac ggugacgacc uucugguugg uacggauuac 6840 gaucuggacu ucaaugaggu ggcgcggcgc gcugccaaac ugggguauaa gaugacuccu 6900 gccaacaaag guucugucuu cccuccgacu uccucucucu ccgacgcugu uuuucuaaaa 6960 cgcaaauucg uccaaaacaa ugacggcuua uacaaaccag uuauggauuu aaagaauuug 7020 gaagccaugc ucuccuacuu caaaccagga acacuacucg agaagcugca aucuguuucu 7080 auguuggcuc aacauucugg aaaagaagaa uaugauagau ugaugcaccc cuuugcugac 7140 uacggugccg uaccgaguca cgaguaccug caggcaagau ggagggccuu guucgacuga 7200 ccuagauagc ccaacgcgcu ccggugcugc cggcgauucu gggagaacuc agucggaaca 7260 gaaaagggaa 7270 <210> 8 <211> 7270 <212> RNA <213> Seneca virus <400> 8 cugggcccuc augcccaguc cuuccuuucc ccuuccgggg gguaaaccgg cuguguuugc 60 uagaggcaca gaggagcaac auccaaccug cuuuugugg gaacggugcg gcuccaauuc 120 cugcgucgcc aaagguguua gcgcacccaa acggcgcauc uaccaaugcu auuggugugg 180 240 cuguaacuac aagauuuagc ccucgcacga gaugugcgau aaccgcaaga uugacuaag 300 cgcggaaagc gcuguaacca caugcuguua gucccuucau ggcugcgaga uggcuaucca 360 ccucggauca cugaacugga gcucgacccu ccuuaguaag ggaaccgaga ggccuucuug 420 caaagcuc cgacacagag uccacgugau ugcuaccacc augaguacau gguucuccccc 480 540 600 gccugauagg gcgacggccu agucgucg guucuauagg uagcacauac aauauugcag 660 aacucucauu uuucuuucga uacagcccu ggcaccuuug aagacguaac cggaacaaaa 720 780 uuagagauac ugaaccuaca gggugaaauu gaauuuuaa agucuuuucaa ugaauaccaa 840 auucgcgccg ccaaacaaca acuuggacug gacaucguau acgaacuaca ggguaauguu 900 cagacaaccu caaagaauga uuuugauucc cgcgguaaua augguaacau gaccuucaau 960 uacuacgcaa acacuuacca gaauucagua gacuucucga ccccucgguc ggcgucaggc 1020 gccggacccg ggaacucccg gggcggauua gcgggucucc ucacaaauuu caguggaauc 1080 uugaacccuc uuggcuaccu caaagaucac aauaccgaag aauggaaaa cucugcugau 1140 cgagucauaa cgcaaacggc gggcaacacu gccauaaaca cgcaaucauc acugggugug 1200 uugugcccu acguugaagaa cccgaccaaa ucugacccuc cguccagcag cacagaucaa 1260 cccaccacca cuuuuaacugc caucgacagg uggucacug gacgccucaa uucuggaca 1320 aaagcuguaa aaaccuucuc uuuucaggcc gucccgcucc cuggagccuu ccugucuaga 1380 cagggaggcc ucaacggagg ggccuucacg gcuacccuac auagacauuu cuuaaugaag 1440 ugcggguggc aggugcaggu ccaaugcaau uugacgcaau uccaccaagg ugcucuucuu 1500 guugccaugg uccccgaaac cacccuugau gucaaaccug acggcaaggc aaagagcuua 1560 caggagcuga augaagaaca guggguggaa augucugacg auuaccggac cgggaaaaac 1620 augccuucc agucucuugg cacauacuau cggcccccua acuggacuug gggccccaau 1680 uucaucaacc ccuaucaagu aacaguuuuc ccacaccaaa uucugaacgc gagaaccucu 1740 accucgguag acauaagugu cccauacauc ggggagacuc cuacaacaauc cucagagaca 1800 cagaacuccu ggacccuccu cguuauggug cuugucccccc uggacuacaa ggagggagcc 1860 acaacugacc cagaaauuac auuuucugua aggcuacaa guccuuauuu caaugggcua 1920 cguaaccguu ucacgaccgg gacggacgag gaacaggggc ccauucccac agcacccaga 1980 gaaaauucgc uuauguuucu cucaaccauc ccugacgaca cugucccugc uuacgggaau 2040 gugcguaccc cucccgucaa uuaccucccu ggugaaauaa ccgaccucuu acaacuggcc 2100 cguauaccca cucuauggc guuugggcgg gugucugaac ccgagccugc cucagacaca 2160 uaugugcccu acguugccgu uccugcccag uucgacgaca agccucucau cuccuucccg 2220 aucacccuuu cagauccugu cuaccagaac acccugguag gcgccaucag uucgaauuuc gccaacuacc ggggguguau gccaaucacu cugacauuuu gccaaccau gauggcaaga gggaaauucc uacucucgua uucuccacca aauggagcac aaccacagac ccuuucuga gcuaugcagu gcacguacuc uauuugggau auaggcuuga acucuaguug gaccuuuguc auccccuaca ucucgcccag ugauuaccgu gaaacucggg cuauuacca cucuguuuauau 2580. ucugcugaug guugguuuag ccugcacaag cuaacuaaaa uuacucuacc accugacugc 2640. cccagaguc ccuguauucu cuuuuucgcc ucugcuggug aggauuacac ccuccgucuc ccuguugauu guaauccuuc cuacguguuc cacuccaccg acaacgccga gacugggguu auugaggcgg guacacuga caccgauuuc ucuggugagc uggcggcucc uggcucuaac 2820. 2820. 2820. 2820. 2820. 2820. 2820 gagaaggacg ccgucuuccc ccguccuuuc cccacagcaa caggugcaca gcaggacgau gguaacuuuu gccuucuaac accccgccca acagucgcuu cccgacccgc cacccguuc ggccuguacg ucaauccguc ugacaguggc guucucgcua acacuucacu ggauuucaau 3000 uuuuacagcu uggccuguuu cacuuacuuu agaucagacc uugaagucac gguggucuca 3060 cggagccag aucuggaauu cgcuguaggg ugguuccccu cuggcaguga guaccaggcu 3120 uccagcuuug ucuacgacca acugcaugua cccuaccacu uuacugggcg cacuccccgc 3180 gcuuucacca gcaagggugg aaagguaucu uucgugcuuc cuuggaacuc ugucucaucc 3240 gugcuucccg ugcgcugggg gggcgcuucc aagcuuucuu ccgccacgcg gggucuaccg 3300 gcuaugcug acugggggac cauuuacgcc uucauccccc guccuaacga gaaaaagc 3360 accgcuguaa agcacguggc gguguacguu cgguacaaga acgcgcgugc cuggugcccc 3420 3480 accaacccug gcccugcuuc ugacaaccca aucuuggagu uucuugaagc ugaaaacgau 3540 cuacuacuc uggcucucu cuggagaaug guacacucug uucaacagac cuggagaaag 3600 uaugugaaaa acgacaauuu uuggcccaau uugcucagug agcuagugg ggaaggcucc 3660 aucgccuugg ccgccacgcu aucuaaccaa gcuucaguaa aagcucucuu aggccugcau 3720 uuucucuccc gagggcucaa uuauacagau uuuaacucuu uacugauaga gaaaugcucu 3780 aguuucuuua cuguagaacc gccuccucca ccagcugaaa aucugaugac caagcccucc 3840 gugaggucga aauuccgaaa gcuguuuaag augcaaggac ccauggacac agucaaagac 3900 uggaaccaaa uagccgccgg ccugaagaau uuccaauuug uucgugaccu ggucaaagag 3960 guggucgauu ggcuccaggc cuggauuaau aaagagaaag ccagcccugu ccuccaguac 4020 cagcuggaga ugagaaacu cggacccgug gcuuggcuc augaugccuu cauggccggu 4080 uccgggcccc cucuuaguga cgaccagauu gaauaucucc agaaccucaa aucucuugcc 4140 cuaacugg ggagacuaa uuuggcccaa agucucacca cuaugaucaa ugccaagcaa 4200 4260 aagagcuugg ccuccacguu gauugcccag gccgugucca agcgucucua cggcucacaa 4320 aguguguauu cucuuccucc ggauccagac uucuucgacg gauacaaagg acaguuugua 4380 4440. accuugaugg acgaucuggg acaaaacccg gaugggcaag auuucuccac cuuuugucag auggugucga ccgcccaauu ucuucccaac auggcggacc uugcagaaaa ggggcguccc 4560. uucaccucca aucuuaucau ugcaacuaca aaccucccuc acuucagccc ugucaccauu 4620. ccgcagucuc ccgcagucuc ccgcagucuc gccuauaaga agcacacacg gcugaauuuu gaccuggcuu ucagacgcac agacgccccc cccauuuauc cuuuugcugc ccaugugccc uucguggacg uggcugugcg cuucaaaaau ggucaucaga gcuucaaucu ccuagaguug gucgacucua uuugugcaga cauucgagcc aagcaacaag gugcucgaaa uaugcagacu cugguucuac agagccccaa cgagaacgac gacacccccg ucgacgaggc guuggguaga guucucaccc ccgcugcggu cgacgaggcg snowflake snowflake snowflake snowflake snowflake snowflake 5040. ggucuugccc uagcugcggu caccccuggu uugauaaucu uggcaguggg acucuacagg uacuucucug gcucugauac 5100. uacuucucug gcucugauac ccuaggagcg agaaugcuua ugacggcccg aagaaaaacu ccaagcccc uggagcgcuc ucccuuaugg aaaugcaaca gcccaacgug gacaugggcu uugaggcugc ggucgcuaag aaaguggucg uccccauuac snow cccaacagac snow uacacagucc gcucuucuug uggccggccg cucumber auuaaugagc auacaugguc caaccccucc uggaccagcu ucacaauccg uggaccagcu cacgcucgug augagccuuu ccaaacgguu 5460. 5460. 5460. 5460. 5460. 5460. 5460. 5460. 5460 5520. ucuuucccua acaaucuaga caaguuugga ugccggcacg uaacucccgu snow uuucggcuag uuacggcaac snow snow snow snow snow snow snow snow snow snow snow snow snow snow acuuacaagg gauggugcgg uucggcccug guuugugagg ccgguggugu ccggcgcauc auuggccugc auucugcugg ugccgcuggu aucggcgccg ggacuuacau cucaaaauua ggacugauca aagcccucaa acaccucggu gaaccucugg cuacaaugca agggcugaug acugagcuag agccuggagu caccgugcau guaccccgga aaucuaaauu gagaaagacg 5880 accgcacacg cgguguacaa accggaguuu gaaccugcug uguugucaaa auuugauccc 5940 agacugaaca aggauguuga ccuagaugag gcaauuuggu cuaaacacac ugccaacguc 6000 ccuuaucaac cuccuuuguu cuacacauac augucagagu acgcucaucg gguuuucucc 6060 uuuuuugggaa aagacaauga cauucugacc gucaaagaag caauuuuggg aaucccugga 6120 cuagacccua uggaucccca cacagcuccg ggucugcccu acgccauuag cggcccuucga 6180 cguacugauc ucgucgauuu ugcgaacggc acgguagacc cgauacuggc caugcaaauc 6240 caaaaauucu uagacgguga cuacucugau caugucuucc aaacuuuucu gaaagaugaa 6300 aucagacccu cagagaaggu ccgagcggga aaaacccgca uugucgaugu gccccccug 6360 gcgcacugca uugugggcag aauguugcuu gggcgcuuug ccgccaaguu ucaaucccau 6420 ccuggcuuuc uccuuggcuc ugcuauuggg ucugacccug augucuucug gaccgucaua 6480 ggggcucagc ucgagggaag aaagaacacg uaugacgugg acuacagugc cuuugacucu 6540 ucacacggca cuggcuccuu cgaggcucuc aucucucacu uuuucaccgu ggacaauggu 6600 uuuagcccug cgcugggacc guaucucaga ucccuggcug ucucggugca cgcuuacggc 6660 6720 aacacagugc ucaacaaugu gaucaucagg acugcucugg cauugaccua caaggaauuu 6780 6840 gaucugacu ucaugaggu ggcgcggcgc gcugccaaac ugggguauaa gaugacuccu 6900 gccaacaaag guucugucuu cccuccgacu uccucucucu ccgacgcugu uuuucuaaaa 6960 cgcaaauucg uccaaaacaa ugacggcuua uacaaaccag uuauggauuu aaagaauuug 7020 gaagcaugc ucuccuacuu caaaccagga acacuacucg agaagcugca aucuguuucu 7080 auguuggcuc aacauucugg aaaagaagaa uaugauagau ugaugcaccc cuuugcugac 7140 uacggugccg uaccgaguca cgaguaccug caggcaagau ggagggccuu guucgacuga 7200 ccuagauagc ccaacgcgcu ccggugcugc cggcgauucu gggagaacuc aguggaaca 7260 gaaaagggaa 7270 <210> 9 <211> 7310 <212> RNA <213> Seneca virus <400> 9 uuugaaaugg ggggcugggc ccugaugccc aguccuuccu uuccccuucc gggggguuaa 60 ccggcugugu uugcuagagg cacagagggg caacauccaa ccugcuuuug cggggaacgg 120 ugcggcuccg auuccugcgu cgccaaaggu guuagcgcac ccaaacggcg caccuaccaa 180 uguuauuggu guggucugcg aguucuagcc uacucguuuc ucccccgacc auucacucac 240 ccacgaaaag uguguuguaa ccauaagauu uaacccccgc acgggaugug cgauaaccgu 300 aagacuggcu caagcgcgga aagcgcugua accacaugcu guuagucccu uuauggcugc 360 aagauggcua cccaccucgg aucacugaac uggagcucga cccuccuuag uaagggaacc 420 gagaggccuu cgugcaacaa gcuccgacac agaguccacg ugacugcuac caccaugagu 4 / 80 acaugguucu ccccucucga cccaggacuu cuuuuugaau auccacggcu cgauccagag 540 gguggggcau gaccccuagc auagcgagcu acagcgggaa cuguagcuag gccuuagcgu 600 It should be noted that in the translation of the sequence part, it is just a literal translation. In the context of biology, it may need to be further verified and understood in combination with specific biological knowledge. And there may be some inaccuracies in the translation of the specific virus name "塞内卡病毒", and it is recommended to check with the standard biological name database for more accurate information. Also, in the translated text of line 26, the "4 / 80" in the original text may be incorrect or unclear, and it is translated as "4 / 80" as it is for now.gccuuggaua cugccugaua gggcgacggc cuagucgugu cgguucuaua gguagcacau 660 acaaauaugc agaacucuca uuuuucuuuc gauacagccu cuggcaccuu ugaagaugua 720 accggaacaa aagucaagau cguugaauac cccagaucgg ugaacaaugg uguuuacgau 780 ucgucuacuc auuuggagau acugaaccua cagggugaaa uugaaauuuu aaggucuuuc 840 aaugaauacc aaauucgcgc cgccaaacaa caacucggac uggacaucgu guacgaacua 900 caggguaaug uucagacaac gucaaagaau gauuuugauu cccguggcaa uaaugguaac 960 augaccuuca auuacuacgc aaacacuuau cagaauucag uagacuucuc gaccuccucg 1020 ucggcgucag gcgccggacc cgggaacucc cggggcggau uagcgggucu ccucacaaau 1080 uucaguggaa ucuugaaccc ucuuggcuac cucaaagauc acaacaccga agaaauggaa 1140 aacucugcug aucgagucac aacgcaaacg gcgggcaaca cugccauaaa cacgcaauca 1200 ucauugggug uguugugugc cuacguugaa gacccgacca aaucugaucc uccguccagc 1260 agcacagauc aacccaccac cacuuucacu gccaucgaca ggugguacac uggacgucuc 1320 aauucuugga caaaagcugu aaaaaccuuc ucuuuucagg ccgucccgcu ucccggggcc 1380 uuucugucua ggcagggagg ccucaacgga ggggccuuca cagcuaccu acauagacac 1440 uuuuugauga agugcgggug gcaggugcag guccaaugua auuugacaca auuccaccaa 1500 ggcgcucuuc uuguugccau gguuccugaa accaccuug augucaagcc cgacgguaag 1560 ccaaagagcu uacaggagcu gaaugaagaa cagugggugg aaaugucuga cgauuaccgg 1620 accgggaaaa acaugccuu ucagucucuu ggcacauacu aucggcccccc uaacuggacu 1680 ugggguccca auuucaucaa ccccuauacaa guaacgguuu ucccacacca aauucugaac 1740 gcgagaaccu cuaccucggu agacauaaac gucccauaca ucggggagac ccccacgcaa 1800 1860 aaaggag ccacaacuga cccagaaauu acauuucug uaaggccuac aagucccuac 1920 uucaaugggc uucgcaaccg cuaccggcc gggacggacg aagaacaggg gcccauuccu 1980 acggcaccca gagaaaauuc gcuuauguuu cucucaaccc ucccugacga cacugucccu 2040 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100 uugcaacugg cccgcauacc cacucucaug gcauugagc gggugccuga acccgugccu gccucagaca cauaugugcc cuacguugcc guuccccc aguucgauga caggccucuc aucuccuucc cgaucacccu uucagauccc gucuaucaga acacccuggu uggcgccauc cover ucgccaauua ccgugggugu auccaaauca cucugacauu uuguggaccc augauggcga gaggaauu ccugcucucg uauucucccc caaauggac gcaaccacag 2460. acucuuuccg aagcuaugca gugcacauac ucuauuuggg acauaggcuu gaacucuagu uggaccuucg ucguccccua caucucgccc agugacuacc gugaaacucg agccauuacc aacucgguuu acuccgcuga cover agccugcaca cover aauuacucua ccaccugacu guccgcaaag ucccugcauu cucuuuuucg cucugcugg ugaggauuac 2700. cgacaacgcc 2700. cgacaacgcc gagaccgggg uuauugaggc ggguaacacu gacaccgauu ucucugguga acuggcggcu ccuggcucua accacacuaa ugucaaguuc cuguuugauc gaucucgauu auugaaugua 2820 aucaagguac uggagaagga cgccguuuuc ccccgcccuu ucccuacaca agaaggugcg 2880 cagcaggaug augguuacuu uugucuucug accccccgcc caacagucgc uucccgaccc 2940 gccacucguu ucggccugua cgccaauccg uccggcagug guguucuugc uaacacuuca 3000 cuggacuuca auuuuuauag cuuggccugu uucacuuacu uuagaucgga ccuugagguu 3060 acgguggucu cacuagagcc ggaucuggaa uuugcuguag ggugguuucc uucuggcagu 3120 gaauaccagg cuuccagcuu ugucuacgac cagcugcaug ugcccuucca cuuuacuggg 3180 cgcacucccc gcgcuuucgc uagcaagggu gggaagguau cuuucgugcu cccuuggaac 3240 ucugucucgu cugugcuccc cgugcgcugg gggggggcuu ccaagcucuc uucugcuacg 3300 cggggucuac cggcgcaugc ugauuggggg acuauuuacg ccuuuguccc ccguccuaau 3360 gagaagaaaa gcaccgcugu aaaacacgug gccguguaca uucgguacaa gaacgcacgu 3420 gccuggugcc ccagcaugcu ucccuuucgc agcuacaagc agaagaugcu gaugcaaucu 3480 ggcgauaucg agaccaaucc ugguccugcu ucugacaacc caauuuugga guuucuugaa 3540 gcagaaaaaaug aucuagucac ucuggcccucu cucuggagaa uggugcacuc uguucaacag 3600 accuggagaa aguaugugaa gaacgaugau uuuuggccca auuuacucag cgagcuagug 3660 ggggaaggcu cugucgccuu ggccgccacg cuauccaacc aagcuucagu aaagcucucuu 3720 uugggccugc acuuucucuc ucggggcuc aauuacacug auuuacuc uuuacugaua 3780 3840 accaagcccu cagugaaguc gaauuccga aaacuguuua agaugcaagg acccauggac 3900 aaagucaaag acuggaacca aauagcugcc ggcuugaagaa auuucaauu uguucgugac 3960 cuaucaaag agguggucga uuggcugcag gccuggauca acaaagagaa agccagcccu 4020 guccuccagu accaguugga gaugagaag cucgggccug uggccuuggc ucaugacgcu 4080 uucauggcug guuccgggcc cccucuuagc gacgaccaga uugaauaccu ccagaaccuc 4140 aaaucucuug cccuaacacu ggggaagacu aauuuggccc aaagucucac cacuaugauc 4200 aaugccaaac aaaguucagc ccaacgaguu gaacccguug uggugguccu uagaggcaag 4260 ccgggaugcg gcaagagcuu ggccucuacg uugauugccc aggcugugc caagcgccuc 4320 uauggcuccc aaaguguaua uucucuuccc ccagauccag auuuuucga uggauacaaa 4380 ggacaguucg ugaccuugau ggaugauuug ggacaaaacc cggauggaca aguauucucc 4440 accuuuuguc agaugguguc gaccgcccaa uuucuccccca acauggcgga ccuugcagag 4500 aaagggcguc ccuuuaccuc caaucucauc auugcaacua caaucuccc ccacuucagu 4560 4620 gaaguaucug aggccuacaa gaacacaca cggcugaauu uugacuuggc uuucaggcgc 4680 acagacgccc cccccauuuua uccuuuugcu gcccaugugc ccuuugugga cguagcugug 4740 4800 gacauucgag ccaagcaaca aggugcccga aacaugcaga cucugguucu acagagcccc 4860 4920 gucgaugagg cgcuugucga ccucacucca gaggccgacc cgguuggccg uuuggcuauu 4980 cuugccaagc uaggucuugc ccuagcugcg gucaccccug gucugauaau cuuggcagug 5040 ggacuuca gguacuucuc uggcucugau gcagaccaag aagaaacaga aagugaggga 5100 ucugucaagg cacccaggag cgaaaaugcu uaugacggcc cgaagaaaaa cucuaagccc 5160 ccuggagcac ucucucucau ggaaaugcaa cagcccaacg uggacauggg cuuugaggcu 5220 gcggucgcua agaaaguggu cgucccauu accuucaugg uucccaacag accuucuggg 5280 cuuacacagu ccgcucuucu ggugaccggc cggaccuucc uaaucaauga acauacaugg 5340 uccaaucccu ccuggaccag cuucacaauc cgcggugagg uacacacucg ugaugagccc 5400 uuccaaacgg uucauuac ucaccacggu auucccacag aucugaugau gguacgucuc 5460 ggaccgggca auucuuuccc uaacaaucua gacaaguuug gacuugacca gaugccggca 5520 cgcaacuccc gugguugg cguuucgucc aguuacggaa acuucuucu cucuggaaau 5580 uuccucggau uuguugauuc caucaccucu gaacaaggaa cuuacgcaag acucuuuagg 5640 uacaggguga cgaccuacaa aggauggugc ggcucggccc uggucuguga ggccgguggc 5700 guccgacgca ucauuggccu gcauucugcu ggcgccgccg guaucggcgc cgggaccuau 5760 aucucaaaau uaggacuaau caaagcccug aaacaccucg gugaaccuuu ggccacaaug 5820 caaggacuga ugacugaauu agagccugga aucaccguac auguaccccg gaaauccaaa 5880 uugaaaga cgaccgcaca cgcgguguac aaacggagu uugagccugc uguguuguca 5940 aaauuugauc ccagacugaa caaggauguu gacuuggaug aaguaauuug gucuaaacac 6000 6060 cgagucuucu ccuucuuggg gaagacaau gacauucuga ccgucaaaga agcaauucug 6120 ggcauccccg gacuagaccc cauggauccc cacagcuc cgggucgcc uuacgccauc 6180 6240 gcuguacaaa uccagaaauu cuuagacggu gacuacucug accaugucuu ccaacuuuu 6300 cugaaagaug agaucagacc cucagagaaa guccgagcgg gaaaacccg cauuguugau 6360 gugcccuccc uggcgcauug cauuguggggc agaaguugc ugggcgcuu ugcugccaag 6420 uuucaauccc auccuggcuu ucuccucggc ucugcuaucg gucugaccc ugauguuuuc 6480 uggaccguca uaggggcuca acucgagggg agaagaaca cguauugacgu ggacuacagu 6540 gccuuugacu cucacacgg cucuggcucc ucgaggcucc cuuuuucacc 6600 guggacaug guuuuagcccc ugcgcuggga ccguaucuca gaucccuggc ugucucggug 6660 cacgcuuacg gcgagcgucg caucaagou accgguggcc uccccuccgg uugugccgcg 6720 accagccugc ugacacagu gcucaacaau gugaucauca ggcugcucu ggcauugacu 6780 uacaggaau uugaauauuga caugguugau aucaucgccu acggugacga ccucugguu 6840 ggcacggauu acgaucugga cucaugag guggcacgac gcgcugcca guuggguau 6900 aagaugacuc cugccaacaa ggguucguc uucccuccga cuccucucu uuccgaugcu 6960 guuuuucuaa agcgcaauu cguccaaaac aacgacggcu uauacaaacc aguauuggau 7020 uuaaagaauu uggaaccau gcucuac uuaaaaaaaaaacuacu cgagaagcug 7080 caaucuguuu cuauguuggc ucaacauucu ggaaaagaag aauaugauag auugaugcac 7140 cccuucgcug acuacggugc cguaccgagu cacgaguacc ugcaggcaag auggagggcc 7200 uuguucgacu gacccagaua gcccaaggcg cuucggugcu gccggcgauu cugggagaac 7260 ucagucggaa cagaaaaggg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 7310 <210> 10 <211> 7356 <212> RNA <213> Seneca virus <400> 10 aggggggggc ugggcccuca ugcccagucc uuccuuuccc cuuccggggg guaaaccggc 60 uguguuugcu agaggcacag aggagcaaca cccaaccugc uuuugugggg aacggugcgg 120 cuccgauucc ugcgucgcca aagguguuag cgcacccaaa cggcgcaucu accaaugcua 180 uugguguggu cugcgaguuc uagccuacuc guuucucccc uauccauuca cucacgcaca 240 aaaagugugu uguaacuaca agauuuagcc cucgcacgag augugcgaua accgcaagac 300 ugacucaagc gcggaaagcg cuguaaccac augcuguuag ucccuuuaug gcugcgagau 360 ggcuauccac cucggaucac ugaacuggag cucgacccuc cuuaguaagg gaaccgagag 420 gccuucuugc aacaagcucc ghacacagagu ccacgugauu gcuaccacca ugaguacaug 480 guucuccccu cucgacccag gacuucuuuu ugaauaucca cggcucgauc cagagggggg 540 ggcaugaucc cccuagcaua gcgagcuaca gcgggaacug uagcuaggcc uuagcgugcc 600 uuggauacug ccugauaggg cgacggccua gucgugucgg uucuauaggu agcacauaua 660 aauaugcaga acucucauuu uucuuucgau acagccucug gcaccuuuga agacguaacc 720 ggaaaaag ucaagaucgu ugaauacccc agaucgguga acauggugu uuacgauucg 780 uccacucauu uagagauacu gaaccuacag ggugaaauug aauuuuaag gucuuucaau 840 gaauaaccaa uucgcgccgc eeaaaaaaaaaaaaaaaaaaaacuacugg 900 gguaaguuc agacaaccuc aaagaauugau uuugauuccc gcggcaauaa cgguaacaug 960 accuucaauu acuacgcaa cacuuaucag auucaguag acucucgac cuccucgucg 1020 gcgucaggcg ccgggcccgg gaacucccgg ggcggauuag cgggucuccu cacaauuuc 1080 aguggaacu ugaacccucu uggcuaccuc aaagaucaca auaccgaaga auggaaaac 1140 ucugcugauc gagucauaac gcaaacggcg ggcaacacug ccauaaacac gcaaucauca 1200 cugggugu uggugugccua uggagaagac ccgaccaaau cugacccucc guccagcagc 1260 acagaucaac ccaccaccac uuuuacugcc aucgacaggu gguacacugg acgccucaau 1320 ucuuggacaa aagcuguaaa aaccuucucu uuucaggccg ucccgcuccc uggagccuuc 1380 cugucuagac agggaggccu caacggaggg gccuucacgg cuacccuaca cagacauuuc 1440 uuaaugaagu gcggguggca gguggcagguc caaugcaauu ugacacaauu ccaccaaggu 1500 gcucuucuug uugccauggu ccccgaaacc acccuygaug ucaagcccga cggcaaggca 1560 aagagccuac aggagcugaa ugaagagcag uggguagaaa ugucugacga uuaccggacc 1620 gggaaaaaaca ugccuuuca gucucuuggc acauacuauc ggcccccuaa cuggacuugg 1680 ggcccuaauu ucaucaaccc cuaucaagua acaguuuucc cacaccaaau ucugaacgcg 1740 agaaccucua cccgguaga cauaaguguc ccauacaucg gggagacucc uacacaaucc 1800 ucagagacac agaacuccug gacccuccuc guuauggugc uugucccccu ggacuacaag 1860 gagggagcca caacugaccc agaaauuaca uuuucuguaa ggccuacaag uccuuacuuc 1920 aaugggcuuc guaaccguua cacgaccggg acggacgagg aacaggggcc cauucccaca 1980 gcacccagag aaaauucgcu uauguuucuc ucaaccaucc cugacgacac ugucccugcu 2040 uacgggaaug ugcguacccc ucccgucaau uaccucccug gugaaauaac cgaccucuua 2100 caacuggccc guauacccac ucucauggcg uuugggcggg ugucugaacc cgagccugcc 2160 ucagacgcau augugcccua cguugccguu cccacccagu ucgaugacaa gccucucauc 2220 uccuucccga ucacccuuuc agauccuguc uaccagaaca cccugguagg cgccaucagu 2280 ucgaauuucg ccaacuaccg gggguguauc caaaucacuc ugacauuuug uggacccaug 2340 auggcaagag ggaaauuccu acucucguau ucucccccaa auggaacgca accacagacu 2400 cuuucugaag ccaugcagug cacauacucc auuugggaua uaggcuugaa cucuaguugg 2460 accuuuguca uccccuacau cucgcccagu gacuaccgug aaacucgggc caucaccaac 2520 ucgguuuacu cugccgaugg uugguuuagc cugcacaagc ugaccaaaau cacucuaccg 2580 ccugacugcc cacagaaacc cuguauucuc uuuuucgccu cugcugguga ggauuacacc 2640 cuccgucucc ccguugauug uaauccuucc uacguguucc acuccaccga caacgccgag 2700 acugggguua uugaggcggg uaacacugac accgauuucu cuggugaacu ggcggcuccu 2760 ggcucuaacc acacuaaugu caaguuccug uuugaucgau cucgauuacu gaauguaauu 2820 aagguacugg agaaggacgc cgucuucccc cguccuuucc ccacagaaac aggugcgcag 2880 caggacgaug guuacuuuug ucuucuaaca ccccgcccaa cagucgccuc ccgacccgcc 2940 acucguuucg gccuguacgu caauccgucu gacaguggcg uucucgccaa cacuucacug 3000 gauuucaauu uuuacagcuu ggccuguuuc acuuacuuua gaucagaccu ugaagucacg 3060 guggucucac uggagccaga ucuggaauuu gcuguagggu gguuccccuc uggcagugag 3120 uaccaggcuu ccagcuuugu cuacgaccaa cugcauguac ccuaccacuu uaccgggcgc 3180 acuccccgcg cuuucaccag caaggguggg aagguaucuu ucgugcuccc uuggaacucu 3240 gucucauccg ugcuucccgu gcgcuggggg gcggcuucca agcucucuuc ugccacgcgg 3300 gguuugccgg cgcaugcuga cugggggacu auuuauugcuu ucgucccccg uccuaugag 3360 aagaaaagca ccgcuguaaa gcacguggcc guguacguuc gguacaagaa cgcacgugcc 3420 uggugcccua gcaugcuucc cuucggcagc uacaagchaga agaugcugau gcaugcuggc 3480 gauaucgaga ccaauccugg cccugcuucu vakaacccaa ucuuggaguu ucuugaggca 3540 gaaaacgauc uagucacucu ggcucucuc uggaagaugg uacaccugu uaccucucu 3600 uggagaagu acgugagaa cgacaacuuu uggcccaauu uacucaguga gcuggugggg 3660 gaaggcucca chick 3720 ggccugcau ucucucccg ggggcucau uacacagacu uuuacuccuu acugauagag 3780 aaugcucua guuucuucac uguagaacca cuccuccac cagccgagaa ccugaugacc 3840 aagcccuccg ugagucgaa auccgaag cuguuuaga ugcaagggcc cauggacaaa 3900 write ggaaccaaau agccgccggu uugagaauu uccaauuuu ccgugaccua 3960 gcugcaggcc uggacaaca aggagaagc cgcccugc 4020 cuccaguauc aguuagagau gaagaagcuc gggccugugg cucuggcuca ugaugcuuuc 4080 auggcugguu cugggcccccc ucuuagugac gaucagauug aauaucucca gaaccuuaaa 4140 ucucuuggccc uaacacuggg gaagcuaau uuggcccaaa gucucaccac uaugaucaau 4200 gccaaacaaa guuccgccca acgagucgaa cccguugugg ugguccuuag aggcaagccg 4260 ggaugcggca agagcuuggc cuccacgcug auugcccagg cuguguccaa gcgucucuac 4320 4380 caguuuguaa ccuugaugga ugaucuggga caaaacccgg auggacaaga uuucuccacc 4440 uuuugucaga uggugucgac cgcccaauuu cuccccaaca uggcggaccu ugcagagaag 4500 gggcgucccu ucaccccaa ucuuaucauu gcaacacaa accuccccca cuucagcccu 4560 4620 guaucugagg ccuacaagaa gcauacacgg cugaauuuug accuggcuuu caggcgcaca 4680 gacgcccccc ccauuauaucc uuuugcugcc caugugcccu ucggugacgu agcugugcgc 4740 uucaaaaaug gucaccagaa cuuuaaucuc cuagaguugg ucgacuccau uugugcagac 4800 4860 gagaugaug acacccccgu cgacgaggcg uuggguagag uucucacccc cgcugcgguc 4920 gacgaggcgc uugucgaccu cgcuccagag gccgauccgg uuggccgcuu ggcuauucuu 4980 gccaagcuag gucuugcccu agcugcgguc accccugguu ugauaaucuu ggcaguggga 5040 cuuuacaggu acuucucugg cucugauaca gaccaagaug aaacagaaug ugaggaaucu 5100 accaaagcac cuaggagcga aaaugcuuau gacggcccga agaaaaacuc uaagcccccu 5160 ggagcacucu cucuuaugga aaugcaacag cccaacgugg acaugggcuu ugaggcugcg 5220 gucgcuaaga aaguggucgu ccccauuacc uucaugguuc ccaacagacc cucuggacuu 5280 acacaguccg cucuucuugu ggccggccgg accuuccuaa ucaaugagca cacauggua 5340 5400 5460 ccgggcaacua cuuccuaaaacuagac ugaccagau gccggcacgc 5520 aacucccgug ugguuggcgu uacgucuagu uacggcaauu ucucuucuc ugggaauuuc 5580 cucggguucg uugacuccau cuccucugaa cucgguagguac uuuuagguac uuuuagguac 5640 agggugacga cuacaaggg cuggugcggc ucggcccugg ucugugaggc cgguggguc 5700 cggcgcauca uuggccugca uucugcuggu gccgcuggua uuggccgg gaccuacauc 5760 ucaaauuag gacugaucaa agcucugaaa caccucggug aaccucuggc uacaugcaa 5820 ggacugauga cugaguuaga gccuggauc accguacaug uacccggaa aucuaaauug 5880 agaaagacga ccgcacacgc aguguacaa ccggaguuug aaccugcugu gugucaaa 5940 uuugauccca gacugaaca ggauguugac uaagaugagg uaauuugguc uaaacacacu 6000 gccaacgucc cuaucauc uccuuuguuc uacacauaca ugucagagua cgcucaucga 6060 guuuucuccu ucuugggaa agacaugac aucugaccg ucaagaagc auccuggggc 6120 aucccuggac uagaccccau ggauccccac aaccuccgg gucugcccua uggcuggac 6180 ggccuucgac guacugaucu cgucgacuuu gugaacggca cgguagacgc agcgcuggcc 6240 augcaaaucc agaaauucuu agacggugac uacucugauc augucuucca aacuuucug 6300 aaagaugaaa ucagacccuc agaaagguc cgagcgggaa aaacccgcau ugucgaugug 6360 cccucccugg cgcauugcau ugugggcaga augcugcuug ggcgcuugc cgccaaguuu 6420 6480 accgucauag gggcucagcu cgagggaaga aagaacacgu augacgugga uaacagugcc 6540 uuugacucuu cacacggcac uggcuccuuc gagggcucuca ucucucacuu uuucaccgug 6600 gacaaugguu uuagcccugc gcugggaccg uaucucagau cccuggcugu cucggugcac 6660 gcuuacggcg agcgucgcau caagauuacc gguggccucc ccuccgguug ugccgcgacc 6720 agcuugcuga acacagugcu caacaaugug aucaucagga cugcucuggc auugacuuac 6780 aaaggaauuug aguaugacau gguugauauc aucgccuacg gugacgaccu ucugguuggu 6840 acggauacg aucuggacuu caugaggug gcacgacgcg cugccaaacu gggguauaag 6900 augacucccg ccaacaaagg uuccgucuuc ccuccgacuu ccucucucuc cgaugcuguu 6960 uuucuaaaac gcaaauucgu ccaaaacaau gauggcuuau acaaaccagu uauggauuua 7020 aagaauuugg aagccaugcu cuccuacuuc aaaccaggaa cacuacucga gaagcugcaa 7080 ucuguuucua uguuggcuca acauucugga aaagaagaau augauagauu gaugcacccc 7140 uucgcugacu acggugccgu accgagucac gaguaccugc aggcaagaug gagggccuug 7200 uucgauugac ccagauagcc caaggcgcuu cggugcugcc ggcgauucug ggagaacuca 7260 gucggaacag aaaagggaaa aaaaaaawaa aaaaaaraaa aaaacaaaaa auaaaaacaa 7320 aaauaaacac gaccucacua cgagagcuga gacugc 7356 <210> 11 <211> 7310 <212> RNA <213> Seneca virus <400> 11 uuugaaaugg ggggcugggc ccugaugccc aguccuuccu uuccccuucc gggggguuaa 60 ccggcugugu uugcuagagg cacagagggg caacauccaa ccugcuuuug cggggaacgg 120 ugcggcuccg auuccugcgu cgccaaaggu guuagcgcac ccaaacggcg caccuaccaa 180 uguuauuggu guggucugcg aguucuagcc uacucguuuc ucccccgacc auucacucac 240 ccacgaaaag uguguuguaa ccauaagauu uaacccccgc acgggaugug cgauuaaccgu 300 aagacuggcu caagcgcgga aagcgcugua accacaugcu guuagucccu uuauggcugc 360 aagauggcua cccaccucgg aucacugaac uggagcucga cccccuuag uaaggggaacc 420 gagaggccuu cgugcaacaa gcuccgacac agaguccacg ugacugcuac caccaugagu 480 acauggucu cccccucucga cccaggacuu cuuuuuugaau auccacggcu cgauccagag 540 gguggggcau gaccccuagc auagcgagcu acagcgggaa cuguagcuag gccuuagcgu 600 gccuuggaua cugccugaua gggcgacggc cuagucgugu cgguucuaua gguagcacau 660 acaauauagc agaacucuca uuuucuuuc gauacagccu cuggcaccuu ugaagaaguagua 720 accggaacaa aagucaagau cguugaauac cccagaucgg ugaacaaugg uguuuacgau 780 ucgucuacuc auuuggagau acugaaccua cagggugaaa uugaaauuuu aaggucuuuc 840 aaugaauacc aaauucgcgc cgccaaaca caacucggac uggacaucgu guacgaacua 900 caggguaaug uucagacaac gucaaagaau gauuuugauu cccguggcaa uaaugguaac 960 augaccuuca auuacuacgc aaacacuuau cagaauucag uagacuucuc gaccuccucg 1020 ucggcgucag gcgccggacc cgggaacucc cggggcggau uagcgggucu ccucacaaau 1080 uucaguggaa ucuugaaccc ucuuggcuac cucaaagauc acaacaccga agaaauggaa 1140 aacucugcug aucgagucac aacgcaaacg gcgggcaaca cugccauaaa cacgcaauca 1200 ucauugggug uguugugugc cuacguugaa gacccgacca aaucugaucc uccguccagc 1260 agcacagauc aacccaccac cacuuucacu gccaucgaca ggugguacac uggacgucuc 1320 aauucuugga caaaagcugu aaaaaccuuc ucuuuucagg ccgucccgcu ucccggggcc 1380 uuucugucua ggcagggagg ccucaacgga ggggccuuca cagcuacccu acauagacac 1440 uuuuugauga agugcgggug gcaggugcag guccaaugua auuugacaca auuccaccaa 1500 ggcgcucuuc uuguugccau gguuccugaa accacccuug augucaagcc cgacgguaag 1560 gcaaagagcu uacaggagcu gaaugaagaa cagugggugg aaaugucuga cgauuaccgg 1620 accgggaaaa acaugccuu ucagucucuu ggcacauacu aucggcccccc uaacuggacu 1680 ugggguccca auuucaucaa ccccuauacaa guaacgguuu ucccacacca aauucugaac 1740 gcgagaaccu cuaccucggu agacauaaac gucccauaca ucggggagac ccccacgcaa 1800 1860 aaaggag ccacaacuga cccagaaauu acauuucug uaaggccuac aagucccuac 1920 uucaaugggc uucgcaaccg cuaccggcc gggacggacg aagaacaggg gcccauuccu 1980 acggcaccca gagaaaauuc gcuuauguuu cucucaaccc ucccugacga cacugucccu 2040 gcuuacggga augugcguac cccccuguc aauuaccucc cuggugaaau aaccgaccuu 2100 2160 2220 aucucccuucc cgaucacccu uucagauccc gucuauaga aacccucgu uggcgccauc 2280 aguucaaauu ucgccaauua ccguggugu auccaaauca cucugacauuu uuguggaccc 2340 augauggcga gaggaauu ccugcucucg uauucucccc caaauggac gcaaccacag 2460. acucuuuccg aagcuaugca gugcacauac ucuauuuggg acauaggcuu gaacucuagu uggaccuucg ucguccccua caucucgccc agugacuacc gugaaacucg agccauuacc aacucgguuu acuccgcuga cover agccugcaca cover aauuacucua ccaccugacu guccgcaaag ucccugcauu cucuuuuucg cucugcugg ugaggauuac 2700. cgacaacgcc 2700. cgacaacgcc gagaccgggg uuauugaggc ggguaacacu gacaccgauu ucucugguga acuggcggcu ccuggcucua accacacuaa ugucaaguc cuguuugauc gaucucgauu auugaaugua aucaagguac uggagaagga cgccguuuuc ccccgcccuu ucccuacaca agaaggugcg cagcaggaug augguuacuu uugucuucug accccccgcc caacagucgc uucccgaccc gccacucguu ucggccugua cgccaauccg uccggcagug uaacacuuca snow auuuuuauag snow snow uuagaucgga snow acgguggucu cacuagagcc ggaucuggaa uuugcuguag ggugguuucc uucuggcagu 3120 gaauaccagg cuuccagcuu ugucuacgac cagcugcaug ugcccuucca cuuuacuggg 3180 cgcacucccc gcgcuuucgc uagcaagggu gggaagguau cuuucgugcu cccuuggaac 3240 ucugucucgu cugugcuccc cgugcgcugg gggggggcuu ccaagcucuc uucugcuacg 3300 cggggucuac cggcgcaugc ugauuggggg acuauuuacg ccuuuguccc ccguccuaau 3360 gagaagaaaa gcaccgcugu aaaacacgug gccguguaca uucgguacaa gaacgcacgu 3420 gccuggugcc ccagcaugcu ucccuuucgc agcuacaagc agaagaugcu gaugcaaucu 3480 ggcgauaucg agaccaaucc ugguccugcu ucugacaacc caauuuugga guuucuugaa 3540 gcagaaaaug aucuagucac ucuggccucu cucuggaaga uggugcacuc uguucaacag 3600 accuggagaa aguaugugaa gaacgaugau uuuuggccca auuuacucag cgagcuagug 3660 ggggaaggcu cugucgccuu ggccgccacg cuauccaacc aagcuucagu aaaggcucuu 3720 uugggccugc acuuucucuc ucgggggcuc aauuacacug acuuuuacuc uuuacugaua 3780 3840 accaagcccu cagugaaguc gaauuccga aaacuguuua agaugcaagg acccauggac 3900 aaagucaaag acuggaacca aauagcugcc ggcuugaagaa auuucaauu uguucgugac 3960 cuaucaaag agguggucga uuggcugcag gccuggauca acaaagagaa agccagcccu 4020 guccuccagu accaguugga gaugagaag cucgggccug uggccuuggc ucaugacgcu 4080 uucauggcug guuccgggcc cccucuuagc gacgaccaga uugaauaccu ccagaaccuc 4140 aaaucucuug cccuaacacu ggggaagacu aauuuggccc aaagucucac cacuaugauc 4200 aaugccaaac aaaguucagc ccaacgaguu gaacccguug uggugguccu uagaggcaag 4260 ccgggaugcg gcaagagcuu ggccucuacg uugauugccc aggcugugc caagcgccuc 4320 uauggcuccc aaaguguaua uucucuuccc ccagauccag auuuuucga uggauacaaa 4380 ggacaguucg ugaccuugau ggaugauuug ggacaaaacc cggauggaca aguauucucc 4440 accuuuuguc agaugguguc gaccgcccaa uuucuccccca acauggcgga ccuugcagag 4500 aaagggcguc ccuuuaccuc caaucucauc auugcaacua caaucuccc ccacuucagu 4560 4620 gaaguaucug aggccuacaa gaacacaca cggcugaauu uugacuuggc uuucaggcgc 4680 acagacgccc cccccauuuua uccuuuugcu gcccaugugc ccuuugugga cguagcugug 4740 4800 gacauucgag ccaagcaaca aggugcccga aacaugcaga cucugguucu acagagcccc 4860 4920 gucgaugagg cgcuugucga ccucacucca gaggccgacc cgguuggccg uuuggcuauu 4980 cuugccaagc uaggucuugc ccuagcugcg gucaccccug gucugauaau cuuggcagug 5040 ggacuuca gguacuucuc uggcucugau gcagaccaag aagaaacaga aagugaggga 5100 ucugucaagg cacccaggag cgaaaaugcu uaugacggcc cgaagaaaaa cucuaagccc 5160 ccuggagcac ucucucucau ggaaaugcaa cagcccaacg uggacauggg cuuugaggcu 5220 gcggucgcua agaaaguggu cgucccauu accuucaugg uucccaacag accuucuggg 5280 cuuacacagu ccgcucuucu ggugaccggc cggaccuucc uaaucaauga acauacaugg 5340 uccaaucccu ccuggaccag cuucacaauc cgcggugagg uacacacucg ugaugagccc 5400 uuccaaacgg uucauuac ucaccacggu auucccacag aucugaugau gguacgucuc 5460 ggaccgggca auucuuuccc uaacaaucua gacaaguuug gacuugacca gaugccggca 5520 cgcaacuccc gugguugg cguuucgucc aguuacggaa acuucuucu cucuggaaau 5580 uuccucggau uuguugauuc caucaccucu gaacaaggaa cuuacgcaag acucuuuagg 5640 uacaggguga cgaccuacaa aggauggugc ggcucggccc uggucuguga ggccgguggc 5700 guccgacgca ucauuggccu gcauucugcu ggcgccgccg guaucggcgc cgggaccuau 5760 aucucaaaau uaggacuaau caaagcccug aaacaccucg gugaaccuuu ggccacaaug 5820 caaggacuga ugacugaauu agagccugga aucaccguac auguaccccg gaaauccaaa 5880 uugaaaga cgaccgcaca cgcgguguac aaacggagu uugagccugc uguguuguca 5940 aaauuugauc ccagacugaa caaggauguu gacuuggaug aaguaauuug gucuaaacac 6000 6060 cgagucuucu ccuucuuggg gaagacaau gacauucuga ccgucaaaga agcaauucug 6120 ggcauccccg gacuagaccc cauggauccc cacagcuc cgggucgcc uuacgccauc 6180 6240 gcuguacaaa uccagaaauu cuuagacggu gacuacucug accaugucuu ccaacuuuu 6300 cugaaagaug agaucagacc cucagagaaa guccgagcgg gaaaacccg cauuguugau 6360 gugcccuccc uggcgcauug cauugugggc agaauguugc uugggcgcuu ugcugccaag 6420 uuucaauccc aucggcuu ucucccggc ucugcuaucg ggucugaccc ugauguuuuc 6480 uggaccguca uaggggcuca acucgagggg agaaagaaca cguaugacgu ggacuacagu 6540 gccuuugacu cuucacacgg cacuggcucc uucgaggcuc ucaucucuca cuuuuuucacc 6600 guggacaaug guuuuagccc ugcgcuggga ccguaucuca gaucccuggc ugucucggug 6660 cacgcuuacg gcgagcgucg caucaagauu accgguggcc uccccuccgg uugugccgcg 6720 accagccugc ugaacacagu gcucaacaau gugaucauca ggacugcucu ggcauugacu 6780 uacaaggaau uugaauauga caugguugau aucaucgccu acggugacga ccuucugguu 6840 ggcacggauu acgaucugga cuucaaugag guggcacgac gcgcugccaa guugggguau 6900 aagaugacuc cugccaacaa ggguucuguc uucccuccga cuuccucucu uuccgaugcu 6960 guuuuucuaa agcgcaaauu cguccaaaac aacgacggcu uauacaaacc aguuauggau 7020 uuaaagaauu uggaagccau gcucuccuac uucaaaccag gaacacuacu cgagaagcug 7080 caaucuguuu cuauguuggc ucaacauucu ggaaaagaag aauaugauag auugaugcac 7140 cccuucgcug acuacggugc cguaccgagu cacgaguacc ugcaggcaag auggagggcc 7200 uuguucgacu gacccagaua gcccaaggcg cuucggugcu gccggcgauu cugggagaac 7260 ucagucggaa cagaaaaggg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 7310 <210> 12 <211> 7310 <212> RNA <213>Séneca virus <400> 12 uuugaaaugg ggggcugggc ccugaugccc aguccuuccu uuccccuucc gggggguuaa 60 ccggcugugu uugcuagagg cacagagggg caacauccaa ccugcuuuug cggggaacgg 120 ugcggcuccg auuccugcgu cgccaaaggu guuagcgcac ccaaacggcg caccuaccaa 180 uguuauuggu guggucugcg aguucuagcc uacucguuuc ucccccgacc auucacucac 240 ccacgaaaag uguguuguaa ccauaagauu uaacccccgc acgggaugug cgauuaaccgu 300 aagacuggcu caagcgcgga aagcgcugua accacaugcu guuagucccu uuauggcugc 360 aagauggcua cccaccucgg aucacugaac uggagcucga cccccuuag uaaggggaacc 420 gagaggccuu cgugcaacaa gcuccgacac agaguccacg ugacugcuac caccaugagu 480 acauggucu cccccucucga cccaggacuu cuuuuuugaau auccacggcu cgauccagag 540 gguggggcau gaccccuagc auagcgagcu acagcgggaa cuguagcuag gccuuagcgu 600 gccuuggaua cugccugaua gggcgacggc cuagucgugu cgguucuaua gguagcacau 660 acaauauagc agaacucuca uuuucuuuc gauacagccu cuggcaccuu ugaagaaguagua 720 accggaacaa aagucaagau cguugaauac cccagaucgg ugaacaaugg uguuuacgau 780 ucgucuacuc auuuggagau acugaaccua cagggugaaa uugaaauuuu aaggucuuuc 840 aaugaauacc aaauucgcgc cgccaaacaa caacucggac uggacaucgu guacgaacua 900 caggguaaug uucagacaac gucaaagaau gauuuugauu cccguggcaa uaaugguaac 960 augaccuuca auuacuacgc aaacacuuau cagaauucag uagacuucuc gaccuccucg 1020 ucggcgucag gcgccggacc cgggaacucc cggggcggau uagcgggucu ccucacaaau 1080 uucaguggaa ucuugaaccc ucuuggcuac cucaaagauc acaacaccga agaaauggaa 1140 aacucugcug aucgagucac aacgcaaacg gcgggcaaca cugccauaaa cacgcaauca 1200 ucauugggug uguugugugc cuacguugaa gacccgacca aaucugaucc uccguccagc 1260 agcacagauc aacccaccac cacuuucacu gccaucgaca ggugguacac uggacgucuc 1320 aauucuugga caaaagcugu aaaaaccuuc ucuuuucagg ccgucccgcu ucccggggcc 1380 uuucugucua ggcagggagg ccucaacgga ggggccuuca cagcuacccu acauagacac 1440 uuuuugauga agugcgggug gcaggugcag guccaaugua auuugacaca auuccaccaa 1500 ggcgcucuuc uuguugccau gguuccugaa accaccuug augucaagcc cgacgguaag 1560 ccaaagagcu uacaggagcu gaaugaagaa cagugggugg aaaugucuga cgauuaccgg 1620 accgggaaaa acaugccuu ucagucucuu ggcacauacu aucggcccccc uaacuggacu 1680 ugggguccca auuucaucaa ccccuauacaa guaacgguuu ucccacacca aauucugaac 1740 gcgagaaccu cuaccucggu agacauaaac gucccauaca ucggggagac ccccacgcaa 1800 1860 aaaggag ccacaacuga cccagaaauu acauuucug uaaggccuac aagucccuac 1920 uucaaugggc uucgcaaccg cuaccggcc gggacggacg aagaacaggg gcccauuccu 1980 acggcaccca gagaaaauuc gcuuauguuu cucucaaccc ucccugacga cacugucccu 2040 gcuuacggga augugcguac cccccuguc aauuaccucc cuggugaaau aaccgaccuu 2100 2160 gccucagaca cauaugugcc cuacguugcc guuccccc aguucgauga caggccucuc aucuccuucc cgaucacccu uucagauccc gucuaucaga acacccuggu uggcgccauc cover ucgccaauua ccgugggugu auccaaauca cucugacauu uuguggaccc augauggcga gaggaauu ccugcucucg uauucucccc caaauggac gcaaccacag 2460. acucuuuccg aagcuaugca gugcacauac ucuauuuggg acauaggcuu gaacucuagu uggaccuucg ucguccccua caucucgccc agugacuacc gugaaacucg agccauuacc aacucgguuu acuccgcuga cover agccugcaca cover aauuacucua ccaccugacu guccgcaaag ucccugcauu cucuuuuucg cucugcugg ugaggauuac 2700. cgacaacgcc 2700. cgacaacgcc gagaccgggg uuauugaggc ggguaacacu gacaccgauu ucucugguga acuggcggcu ccuggcucua accacacuaa covered covered covered covered covered covered covered covered covered covered covered aucaagguac uggagaagga cgccguuuuc ccccgcccuu ucccuacaca agaaggugcg cagcaggaug augguuacuu uugucuucug accccccgcc caacagucgc uucccgaccc 2940 gccacucguu ucggccugua cgccaauccg uccggcagug guguucuugc uaacacuuca 3000 cuggacuuca auuuuuauag cuuggccugu uucacuuacu uuagaucgga ccuugagguu 3060 acgguggucu cacuagagcc ggaucuggaa uuugcuguag ggugguuucc uucuggcagu 3120 gaauaccagg cuuccagcuu ugucuacgac cagcugcaug ugcccuucca cuuuacuggg 3180 cgcacucccc gcgcuuucgc uagcaagggu gggaagguau cuuucgugcu cccuuggaac 3240 ucugucucgu cugugcuccc cgugcgcugg gggggggcuu ccaagcucuc uucugcuacg 3300 cggggucuac cggcgcaugc ugauuggggg acuauuuacg ccuuuguccc ccguccuaau 3360 gagaagaaaa gcaccgcugu aaaacacgug gccguguaca uucgguacaa gaacgcacgu 3420 gccuggugcc ccagcaugcu ucccuuucgc agcuacaagc agaagaugcu gaugcaaucu 3480 ggcgauaucg agaccaaucc ugguccugcu ucugacaacc caauuuugga guuucuugaa 3540 gcagaaaaug aucuagucac ucuggccucu cucuggaaga uggugcacuc uguucaacag 3600 accuggagaa aguaugugaa gaacgaugau uuuuggccca auuuacucag cgagcuagug 3660 ggggaaggcu cugucgccuu ggccgccacg cuauccaacc aagcuucagu aaagcucucuu 3720 uugggccugc acuuucucuc ucggggcuc aauuacacug auuuacuc uuuacugaua 3780 3840 accaagcccu cagugaaguc gaauuccga aaacuguuua agaugcaagg acccauggac 3900 aaagucaaag acuggaacca aauagcugcc ggcuugaagaa auuucaauu uguucgugac 3960 cuaucaaag agguggucga uuggcugcag gccuggauca acaaagagaa agccagcccu 4020 guccuccagu accaguugga gaugagaag cucgggccug uggccuuggc ucaugacgcu 4080 uucauggcug guuccgggcc cccucuuagc gacgaccaga uugaauaccu ccagaaccuc 4140 aaaucucuug cccuaacacu ggggaagacu aauuuggccc aaagucucac cacuaugauc 4200 aaugccaaac aaaguucagc ccaacgaguu gaacccguug uggugguccu uagaggcaag 4260 ccgggaugcg gcaagagcuu ggccucuacg uugauugccc aggcugugc caagcgccuc 4320 uauggcuccc aaaguguaua uucucuuccc ccagauccag auuuuucga uggauacaaa 4380 ggacaguucg ugaccuugau ggaugauuug ggacaaaacc cggauggaca aguauucucc 4440 accuuuuguc agaugguguc gaccgcccaa uuucuccccca acauggcgga ccuugcagag 4500 aaagggcguc ccuuuaccuc caaucucauc auugcaacua caaucuccc ccacuucagu 4560 4620 gaaguaucug aggccuacaa gaacacaca cggcugaauu uugacuuggc uuucaggcgc 4680 acagacgccc cccccauuuua uccuuuugcu gcccaugugc ccuuugugga cguagcugug 4740 4800 gacauucgag ccaagcaaca aggugcccga aacaugcaga cucugguucu acagagcccc 4860 4920 gucgaugagg cgcuugucga ccucacucca gaggccgacc cgguuggccg uuuggcuauu 4980 cuugccaagc uaggucuugc ccuagcugcg gucaccccug gucugauaau cuuggcagug 5040 ggacuuca gguacuucuc uggcucugau gcagaccaag aagaaacaga aagugaggga 5100 ucugucaagg cacccaggag cgaaaaugcu uaugacggcc cgaagaaaaa cucuaagccc 5160 ccuggagcac ucucucucau ggaaaugcaa cagcccaacg uggacauggg cuuugaggcu 5220 gcggucgcua agaaaguggu cgucccauu accuucaugg uucccaacag accuucuggg 5280 cuuacacagu ccgcucuucu ggugaccggc cggaccuucc uaaucaauga acauacaugg 5340 uccaaucccu ccuggaccag cuucacaauc cgcggugagg uacacacucg ugaugagccc 5400 uuccaaacgg uucauuac ucaccacggu auucccacag aucugaugau gguacgucuc 5460 ggaccgggca auucuuuccc uaacaaucua gacaaguuug gacuugacca gaugccggca 5520 cgcaacuccc gugguugg cguuucgucc aguuacggaa acuucuucu cucuggaaau 5580 uuccucggau uuguugauuc caucaccucu gaacaaggaa cuuacgcaag acucuuuagg 5640 uacaggguga cgaccuacaa aggauggugc ggcucggccc uggucuguga ggccgguggc 5700 guccgacgca ucauuggccu gcauucugcu ggcgccgccg guaucggcgc cgggaccuau 5760 aucucaaaau uaggacuaau caaagcccug aaacaccucg gugaaccuuu ggccacaaug 5820 caaggacuga ugacugaauu agagccugga aucaccguac auguaccccg gaaauccaaa 5880 uugaaaga cgaccgcaca cgcgguguac aaacggagu uugagccugc uguguuguca 5940 aaauuugauc ccagacugaa caaggauguu gacuuggaug aaguaauuug gucuaaacac 6000 6060 cgagucuucu ccuucuuggg gaagacaau gacauucuga ccgucaaaga agcaauucug 6120 ggcauccccg gacuagaccc cauggauccc cacagcuc cgggucgcc uuacgccauc 6180 6240 gcuguacaaa uccagaaauu cuuagacggu gacuacucug accaugucuu ccaacuuuu 6300 cugaaagaug agaucagacc cucagagaaa guccgagcgg gaaaacccg cauuguugau 6360 gugcccuccc uggcgcauug cauugugggc agaauguugc uugggcgcuu ugcugccaag 6420 uuucaauccc aucggcuu ucucccggc ucugcuaucg ggucugaccc ugauguuuuc 6480 uggaccguca uaggggcuca acucgagggg agaagaaca cguauugacgu ggacuacagu 6540 gccuuugacu cucacacgg cucuggcucc ucgaggcucc cuuuuucacc 6600 guggacaug guuuuagcccc ugcgcuggga ccguaucuca gaucccuggc ugucucggug 6660 cacgcuuacg gcgagcgucg caucaagou accgguggcc uccccuccgg uugugccgcg 6720 accagccugc ugacacagu gcucaacaau gugaucauca ggcugcucu ggcauugacu 6780 uacaggaau uugaauauuga caugguugau aucaucgccu acggugacga ccucugguu 6840 ggcacggauu acgaucugga cucaugag guggcacgac gcgcugcca guuggguau 6900 aagaugacuc cugccaacaa ggguucguc uucccuccga cuccucucu uuccgaugcu 6960 guuuuucuaa agcgcaauu cguccaaaac aacgacggcu uauacaaacc aguauuggau 7020 uuaaagaauu uggaaccau gcucuac uuaaaaaaaaaacuacu cgagaagcug 7080 caacuguuu quauguuggc ucaucu ggaaagaag auauugauag auugaugcac 7140 cccuucgcug acuacggugc cguaccgagu ccgaguacc ugcaggcag auggaggggcc 7200 uuguucgacu gacccagaua gcccaaggcg cuucggugcu gccggcgauu cugggagaac 7260 ucagucggaa cagaaaaggg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 7310 <210> 13 <211> 7310 <212> RNA <213> Seneca virus <400> 13 uuugaaaugg ggggcugggc ccugaugccc aguccuuccu uuccccuucc gggggguuaa 60 ccggcugugu uugcuagagg cacagagggg caacauccaa ccugcuuuug cggggaacgg 120 ugcggcuccg auuccugcgu cgccaaaggu guuagcgcac ccaaacggcg caccuaccaa 180 uguuauuggu guggucugcg aguucuagcc uacucguuuc ucccccgacc auucacucac 240 ccacgaaaag uguguuguaa ccauaagauu uaacccccgc acgggaugug cgauaaccgu 300 aagacuggcu caagcgcgga aagcgcugua accacaugcu guuagucccu uuauggcugc 360 aagauggcua cccaccucgg aucacugaac uggagcucga cccuccuuag uaagggaacc 420 gagaggccuu cgugcaacaa gcuccgacac agaguccacg ugacugcuac caccaugagu 480 acaugguucu ccccucucga cccaggacuu cuuuuugaau auccacggcu cgauccagag 540 gguggggcau gaccccuagc auagcgagcu acagcgggaa cuguagcuag gccuuagcgu 600 gccuuggaua cugccugaua gggcgacggc cuagucgugu cgguucuaua gguagcacau 660 acaauauagc agaacucuca uuuucuuuc gauacagccu cuggcaccuu ugaagaaguagua 720 accggaacaa aagucaagau cguugaauac cccagaucgg ugaacaaugg uguuuacgau 780 ucgucuacuc auuuggagau acugaaccua cagggugaaa uugaaauuuu aaggucuuuc 840 aaugaauacc aaauucgcgc cgccaaaca caacucggac uggacaucgu guacgaacua 900 caggguaaug uucagacaac gucaaagaau gauuuugauu cccguggcaa uaaugguaac 960 augaccuuca auuacuacgc aaacacuuau cagaauucag uagacuucuc gaccucccg 1020 ucggcgucag gcgccggacc cgggaacucc cggggcggau uagcgggucu ccucacaaau 1080 uucaguggaa ucuugaaccc ucuuggcuac cucaaagauc acaacaccga agaaauggaa 1140 aacucugcug aucgagucac aacgcaaacg gcgggcaaca cugccauaaa cacgcaauca 1200 ucauugggug uguugugugc cuacguugaa gacccgacca aaucugaucc uccguccagc 1260 agcacagauc aacccaccac cacuuucacu gccaucgaca ggugguacac uggacgucuc 1320 aaucuugga aaagcugu aaaaaccuuc ucuuuucagg ccgucccgcu ucccggggcc 1380 uuucugucua ggcagggagg ccucaacgga ggggcuuca ggcuacccu 1440 uuuuugauga agugcgggug gcaggugcag guccaaugua auuugacaca auuccaccaa 1500 ggcgcucuuc uuguugccau gguuccugaa accaccuug augucaagcc cgacgguag 1560 gcaagagcu uacaggagcu gaugagaa cagugggggg aaugucuga cgauuaccgg 1620 accgggaaaa acugccuuu ucagucucuu ggcacauacu aucggccccc uaacuggacu 1680 1740 - uggguccca auuucaucaa ccccuaucaa guaacgguuuuuuucacca 1740 gcgagaaccu cuccucggu agacauaaac cuccauaca ucggggac ccccacgcaa 1800 cuggacuc cuggacuc cucguuaugg cuckoogg cuggacuc 1860 aaggaagg ccaacuga cccagaauu acauuuucug uaaggccuac aagucccuac 1920 uucaugggc uucgcaccg cuacacggcc gggacggacg agaacaggg gcccauccu 1980 acggcaccca gagaaaauuc gcuuauguuu cucucaaccc ucccugacga cacugucccu 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100. 2100 uugcaacugg cccgcauacc cacucucaug gcauugagc gggugccuga acccgugccu gccucagaca cauaugugcc cuacguugcc guuccccc aguucgauga caggccucuc aucuccuucc cgaucacccu uucagauccc gucuaucaga acacccuggu uggcgccauc cover ucgccaauua ccgugggugu auccaaauca cucugacauu uuguggaccc augauggcga gaggaauu ccugcucucg uauucucccc caaauggac gcaaccacag 2460. acucuuuccg aagcuaugca gugcacauac ucuauuuggg acauaggcuu gaacucuagu uggaccuucg ucguccccua caucucgccc agugacuacc gugaaacucg agccauuacc aacucgguuu acuccgcuga cover agccugcaca cover aauuacucua ccaccugacu guccgcaaag ucccugcauu cucuuuuucg cucugcugg ugaggauuac 2700. cgacaacgcc 2700. cgacaacgcc gagaccgggg uuauugaggc ggguaacacu gacaccgauu ucucugguga acuggcggcu 2760 2820 aucaagguac uggaagaagga cgccguuuuc ccccgcccuu ucccuacaca agaaggugcg 2880 cagcaggaug augguuacuu uugucuucug accccccgcc caacagucgc uucccgaccc 2940 gccacucguu ucggcccugua cgccaauccg uccggcagug guguucuugc uaacacuuca 3000 cuggacuuca auuuuuaag cuuggccugu uucacuuacu uuagaucgga ccuugagguu 3060 acgguggucu cacuagagcc ggaucuggaa uuugcuguag ggugguuucc uucuggcagu 3120 gaauaccagg cuuccagcuu ugucuacgac cagcugcaug ugcccuucca cuuuacuggg 3180 cgcacucccc gcgcuuucgc uagcaagggu gggaagguau cuuucgugcu cccuuggaac 3240 ucugucucgu cugugcuccc cgugcgcugg ggggggggcuu ccaagcucuc uucugcuacg 3300 cggggucuac cggcgcaugc ugauugggg acuauuuacg ccuuuguccc ccguccuaau 3360 gagaaaaa gcaccgcugu aaaacacgug gccguguaca uucgguacaa gaacgcacgu 3420 gccuggugcc ccagcaugcu ucccuuucgc agcuacaagc agaagaugcu gaugcaaucu 3480 ggcgauaucg agaccaaucc ugguccugcu ucugacaacc caauuuugga guuucuugaa 3540 gcagaaaaug aucuagucac ucuggccucu cucuggaaga uggugcacuc uguucaacag 3600 accuggagaa aguaugugaa gaacgaugau uuuuggccca auuuacucag cgagcuagug 3660 ggggaaggcu cugucgccuu ggccgccacg cuauccaacc aagcuucagu aaaggcucuu 3720 uugggccugc acuuucucuc ucgggggcuc aauuacacug acuuuuacuc uuuacugaua 3780 gagaaaugcu cuaguuucuu uaccguagaa ccaccuccuc caccagcuga aaaccugaug 3840 accaagcccu cagugaaguc gaaauuccga aaacuguuua agaugcaagg acccauggac 3900 aaagucaaag acuggaacca aauagcugcc ggcuugaaga auuuucaauu uguucgugac 3960 cuagucaaag agguggucga uuggcugcag gccuggauca acaaagagaa agccagcccu 4020 guccuccagu accaguugga gaugaagaag cucgggccug uggccuuggc ucaugacgcu 4080 uucauggcug guuccgggcc cccucuuagc gacgaccaga uugaauaccu ccagaaccuc 4140 aaaucucuug cccuaacacu ggggaagacu aauuuggccc aaagucucac cacuaugauc 4200 aaugccaaac aaaguucagc ccaacgaguu gaacccguug uggugguccu uagaggcaag 4260 ccgggaugcg gcaagagcuu ggccucuacg uugauugccc aggcugugc caagcgccuc 4320 uauggcuccc aaaguguaua uucucuuccc ccagauccag auuuuucga uggauacaaa 4380 ggacaguucg ugaccuugau ggaugauuug ggacaaaacc cggauggaca aguauucucc 4440 accuuuuguc agaugguguc gaccgcccaa uuucuccccca acauggcgga ccuugcagag 4500 aaagggcguc ccuuuaccuc caaucucauc auugcaacua caaucuccc ccacuucagu 4560 4620 gaaguaucug aggccuacaa gaacacaca cggcugaauu uugacuuggc uuucaggcgc 4680 acagacgccc cccccauuuua uccuuuugcu gcccaugugc ccuuugugga cguagcugug 4740 4800 gacauucgag ccaagcaaca aggugcccga aacaugcaga cucugguucu acagagcccc 4860 4920 gucgaugagg cgcuugucga ccucacucca gaggccgacc cgguuggccg uuuggcuauu 4980 cuugccaagc uaggucuugc ccuagcugcg gucaccccug gucugauaau cuuggcagug 5040 ggacuuca gguacuucuc uggcucugau gcagaccaag aagaaacaga aagugaggga 5100 ucugucaagg cacccaggag cgaaaaugcu uaugacggcc cgaagaaaaa cucuaagccc 5160 ccuggagcac ucucucucau ggaaaugcaa cagcccaacg uggacauggg cuuugaggcu 5220 gcggucgcua agaaaguggu cgucccauu accuucaugg uucccaacag accuucuggg 5280 cuuacacagu ccgcucuucu ggugaccggc cggaccuucc uaaucaauga acauacaugg 5340 uccaaucccu ccuggaccag cuucacaauc cgcggugagg uacacacucg ugaugagccc 5400 uuccaaacgg uucauuac ucaccacggu auucccacag aucugaugau gguacgucuc 5460 ggaccgggca auucuuuccc uaacaaucua gacaaguuug gacuugacca gaugccggca 5520 cgcaacuccc gugguugg cguuucgucc aguuacggaa acuucuucu cucuggaaau 5580 uuccucggau uuguugauuc caucaccucu gaacaaggaa cuuacgcaag acucuuuagg 5640 uacaggguga cgaccuacaa aggauggugc ggcucggccc uggucuguga ggccgguggc 5700 guccgacgca ucauuggccu gcauucugcu ggcgccgccg guaucggcgc cgggaccuau 5760 aucucaaaau uaggacuaau caaagcccug aaacaccucg gugaaccuuu ggccacaaug 5820 caaggacuga ugacugaauu agagccugga aucaccguac auguaccccg gaaauccaaa 5880 uugaaaga cgaccgcaca cgcgguguac aaacggagu uugagccugc uguguuguca 5940 aaauuugauc ccagacugaa caaggauguu gacuuggaug aaguaauuug gucuaaacac 6000 6060 cgagucuucu ccuucuuggg gaagacaau gacauucuga ccgucaaaga agcaauucug 6120 ggcauccccg gacuagaccc cauggauccc cacagcuc cgggucgcc uuacgccauc 6180 6240 gcuguacaaa uccagaaauu cuuagacggu gacuacucug accaugucuu ccaacuuuu 6300 cugaagaug agaucagacc cucagagaaa guccgagcgg gaaaacccg cauuguugau 6360 gugcccuccc uggcgcauug cauuguggggc agaaguugc ugggcgcuu ugcugccaag 6420 uuucaauccc auccuggcuu ucuccucggc ucugcuaucg gucugaccc ugauguuuuc 6480 uggaccguca uaggggcuca acucgagggg agaagaaca cguauugacgu ggacuacagu 6540 gccuuugacu cucacacgg cucuggcucc ucgaggcucc cuuuuucacc 6600 guggacaug guuuuagcccc ugcgcuggga ccguaucuca gaucccuggc ugucucggug 6660 cacgcuuacg gcgagcgucg caucaagou accgguggcc uccccuccgg uugugccgcg 6720 accagccugc ugacacagu gcucaacaau gugaucauca ggcugcucu ggcauugacu 6780 uacaggaau uugaauauuga caugguugau aucaucgccu acggugacga ccucugguu 6840 ggcacggauu acgaucugga cucaugag guggcacgac gcgcugcca guuggguau 6900 aagaugacuc cugccaacaa ggguucguc uucccuccga cuccucucu uuccgaugcu 6960 guuuuucuaa agcgcaauu cguccaaaac aacgacggcu uauacaaacc aguauuggau 7020 uuaaagaauu uggaagccau gcucuccuac uucaaaccag gaacacuacu cgagaagcug 7080 caaucuguuu cuauguuggc ucaacauucu ggaaaagaag aauaugauag auugaugcac 7140 cccuucgcug acuacggugc cguaccgagu cacgaguacc ugcaggcaag auggagggcc 7200 uuguucgacu gacccagaua gcccaaggcg cuucggugcu gccggcgauu cugggagaac 7260 ucagucggaa cagaaaaggg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 7310 <210> 14 <211> 7310 <212> RNA <213> Seneca virus <400> 14 uuugaaaugg ggggcugggc ccugaugccc aguccuuccu uuccccuucc gggggguuaa 60 ccggcugugu uugcuagagg cacagagggg caacauccaa ccugcuuuug cggggaacgg 120 ugcggcuccg auuccugcgu cgccaaaggu guuagcgcac ccaaacggcg caccuaccaa 180 uguuauuggu guggucugcg aguucuagcc uacucguuuc ucccccgacc auucacucac 240 ccacgaaaag uguguuguaa ccauaagauu uaacccccgc acgggaugug cgauaaccgu 300 aagacuggcu caagcgcgga aagcgcugua accacaugcu guuagucccu uuauggcugc 360 aagauggcua cccaccucgg aucacugaac uggagcucga cccccuuag uaaggggaacc 420 gagaggccuu cgugcaacaa gcuccgacac agaguccacg ugacugcuac caccaugagu 480 acauggucu cccccucucga cccaggacuu cuuuuuugaau auccacggcu cgauccagag 540 gguggggcau gaccccuagc auagcgagcu acagcgggaa cuguagcuag gccuuagcgu 600 gccuuggaua cugccugaua gggcgacggc cuagucgugu cgguucuaua gguagcacau 660 acaauauagc agaacucuca uuuucuuuc gauacagccu cuggcaccuu ugaagaaguagua 720 accggaacaa aagucaagau cguugaauac cccagaucgg ugaacaaugg uguuuacgau 780 ucgucuacuc auuuggagau acugaaccua cagggugaaa uugaaauuuu aaggucuuuc 840 aaugaauacc aaauucgcgc cgccaaaca caacucggac uggacaucgu guacgaacua 900 caggguaaug uucagacaac gucaaagaau gauuuugauu cccguggcaa uaaugguaac 960 augaccuuca auuacuacgc aaacacuuau cagaauucag uagacuucuc gaccucccg 1020 ucggcgucag gcgccggacc cgggaacucc cggggcggau uagcgggucu ccucacaaau 1080 uucaguggaa ucuugaaccc ucuuggcuac cucaaagauc acaacaccga agaaauggaa 1140 aacucugcug aucgagucac aacgcaaacg gcgggcaaca cugccauaaa cacgcaauca 1200 ucauugggug uguugugugc cuacguugaa gacccgacca aaucugaucc uccguccagc 1260 agcacagauc aacccaccac cacuuucacu gccaucgaca ggugguacac uggacgucuc 1320 aauucuugga caaaagcugu aaaaaccuuc ucuuuucagg ccgucccgcu ucccggggcc 1380 uuucugucua ggcagggagg ccucaacgga ggggccuuca cagcuacccu acauagacac 1440 uuuuugauga agugcgggug gcaggugcag guccaaugua auuugacaca auuccaccaa 1500 ggcgcucuuc uuguugccau gguuccugaa accacccuug augucaagcc cgacgguaag 1560 gcaaagagcu uacaggagcu gaaugaagaa cagugggugg aaaugucuga cgauuaccgg 1620 accgggaaaa acaugccuuu ucagucucuu ggcacauacu aucggccccc uaacuggacu 1680 ugggguccca auuucaucaa ccccuaucaa guaacgguuu ucccacacca aauucugaac 1740 gcgagaaccu cuaccucggu agacauaaac gucccauaca ucggggagac ccccacgcaa 1800 1860 aaaggag ccacaacuga cccagaaauu acauuucug uaaggccuac aagucccuac 1920 uucaaugggc uucgcaaccg cuaccggcc gggacggacg aagaacaggg gcccauuccu 1980 acggcaccca gagaaaauuc gcuuauguuu cucucaaccc ucccugacga cacugucccu 2040 gcuuacggga augugcguac cccccuguc aauuaccucc cuggugaaau aaccgaccuu 2100 2160 2220 aucucccuucc cgaucacccu uucagauccc gucuauaga aacccucgu uggcgccauc 2280 aguucaaauu ucgccaauua ccguggugu auccaaauca cucugacauuu uuguggaccc 2340 augauggcga gaggaaauu ccugcuccg uauucucccc caauggac gcaaccacag 2400 acucuuuccg aagcuaugca gugcacauac ucuauuuggg acauaggcuu gaacucaagu 2460 uggaccuucg ucguccccua caucucgcccc agugacuacc gugaaacucg agccauuacc 2520 aacucgguuu acuccgcuga cover agccugcaca cover aauuacucua ccaccugacu guccgcaaag ucccugcauu cucuuuuucg cucugcugg ugaggauuac 2700. cgacaacgcc 2700. cgacaacgcc gagaccgggg uuauugaggc ggguaacacu gacaccgauu ucucugguga acuggcggcu ccuggcucua accacacuaa covered covered covered covered covered covered covered covered covered covered covered aucaagguac uggagaagga cgccguuuuc ccccgcccuu ucccuacaca agaaggugcg cagcaggaug augguuacuu uugucuucug accccccgcc caacagucgc uucccgaccc gccacucguu ucggccugua cgccaauccg uccggcagug uaacacuuca snow auuuuuauag snow snow uuagaucgga snow acgguggucu cacuagagcc ggaucugga uuugcuguag ggugguuucc uucuggcagu gaauaccagg cuuccagcuu ugucuacgac cagcugcaug ugcccuucca cuuuacuggg cgcacucccc gcgcuuucgc uagcaagggu gggaagguau cuuucgugcu cccuuggaac ucugucucgu cugugcuccc cgugcgcugg gggggggcuu ccaagcucuc uucugcuacg 3300 cggggucuac cggcgcaugc ugauuggggg acuauuuacg ccuuuguccc ccguccuaau 3360 gagaagaaaa gcaccgcugu aaaacacgug gccguguaca uucgguacaa gaacgcacgu 3420 gccuggugcc ccagcaugcu ucccuuucgc agcuacaagc agaagaugcu gaugcaaucu 3480 ggcgauaucg agaccaaucc ugguccugcu ucugacaacc caauuuugga guuucuugaa 3540 gcagaaaaug aucuagucac ucuggccucu cucuggaaga uggugcacuc uguucaacag 3600 accuggagaa aguaugugaa gaacgaugau uuuuggccca auuuacucag cgagcuagug 3660 ggggaaggcu cugucgccuu ggccgccacg cuauccaacc aagcuucagu aaaggcucuu 3720 uugggccugc acuuucucuc ucgggggcuc aauuacacug acuuuuacuc uuuacugaua 3780 gagaaaugcu cuaguuucuu uaccguagaa ccaccuccuc caccagcuga aaaccugaug 3840 accaagcccu cagugaaguc gaaauuccga aaacuguuua agaugcaagg acccauggac 3900 aaagucaaag acuggaacca aauagcugcc ggcuugaaga auuuucaauu uguucgugac 3960 cuaucaaag agguggucga uuggcugcag gccuggauca acaaagagaa agccagcccu 4020 guccuccagu accaguugga gaugagaag cucgggccug uggccuuggc ucaugacgcu 4080 uucauggcug guuccgggcc cccucuuagc gacgaccaga uugaauaccu ccagaaccuc 4140 aaaucucuug cccuaacacu ggggaagacu aauuuggccc aaagucucac cacuaugauc 4200 aaugccaaac aaaguucagc ccaacgaguu gaacccguug uggugguccu uagaggcaag 4260 ccgggaugcg gcaagagcuu ggccucuacg uugauugccc aggcugugc caagcgccuc 4320 uauggcuccc aaaguguaua uucucuuccc ccagauccag auuuuucga uggauacaaa 4380 ggacaguucg ugaccuugau ggaugauuug ggacaaaacc cggauggaca aguauucucc 4440 accuuuuguc agaugguguc gaccgcccaa uuucuccccca acauggcgga ccuugcagag 4500 aaagggcguc ccuuuaccuc caaucucauc auugcaacua caaucuccc ccacuucagu 4560 4620 gaaguaucug aggccuacaa gaacacaca cggcugaauu uugacuuggc uuucaggcgc 4680 acagacgccc cccccauuuua uccuuuugcu gcccaugugc ccuuugugga cguagcugug 4740 4800 gacauucgag ccaagcaaca aggugcccga aacaugcaga cucugguucu acagagcccc 4860 4920 gucgaugagg cgcuugucga ccucacucca gaggccgacc cgguuggccg uuuggcuauu 4980 cuugccaagc uaggucuugc ccuagcugcg gucaccccug gucugauaau cuuggcagug 5040 ggacuuca gguacuucuc uggcucugau gcagaccaag aagaaacaga aagugaggga 5100 ucugucaagg cacccaggag cgaaaaugcu uaugacggcc cgaagaaaaa cucuaagccc 5160 ccuggagcac ucucucucau ggaaaugcaa cagcccaacg uggacauggg cuuugaggcu 5220 gcggucgcua agaaaguggu cgucccauu accuucaugg uucccaacag accuucuggg 5280 cuuacacagu ccgcucuucu ggugaccggc cggaccuucc uaaucaauga acauacaugg 5340 uccaaucccu ccuggaccag cuucacaauc cgcggugagg uacacacucg ugaugagccc 5400 uuccaaacgg uucauuac ucaccacggu auucccacag aucugaugau gguacgucuc 5460 ggaccgggca auucuuuccc uaacaaucua gacaaguuug gacuugacca gaugccggca 5520 cgcaacuccc gugguugg cguuucgucc aguuacggaa acuucuucu cucuggaaau 5580 uuccucggau uuguugauuc caucaccucu gaacaaggaa cuuacgcaag acucuuuagg 5640 uacaggguga cgaccuacaa aggauggugc ggcucggccc uggucuguga ggccgguggc 5700 guccgacgca ucauuggccu gcauucugcu ggcgccgccg guaucggcgc cgggaccuau 5760 aucucaaaau uaggacuaau caaagcccug aaacaccucg gugaaccuuu ggccacaaug 5820 caaggacuga ugacugaauu agagccugga aucaccguac auguaccccg gaaauccaaa 5880 uugaaaga cgaccgcaca cgcgguguac aaacggagu uugagccugc uguguuguca 5940 aaauuugauc ccagacugaa caaggauguu gacuuggaug aaguaauuug gucuaaacac 6000 6060 cgagucuucu ccuucuuggg gaagacaau gacauucuga ccgucaaaga agcaauucug 6120 ggcauccccg gacuagaccc cauggauccc cacacagcuc cgggucugcc uuacgccauc 6180 aacggccuuc gacguacuga ucucgucgau uuugugaacg guacaguaga uggcggcgcug 6240 gcuguacaa uccagaaauu cuagacggu gacuacucug accaagucuu ccaacuuuu 6300 cugaagaug agaucagacc cucagagaaa guccgagcgg gaaaacccg cauuguugau 6360 gugcccuccc uggcgcauug cauuguggggc agaaguugc ugggcgcuu ugcugccaag 6420 uuucaauccc auccuggcuu ucuccucggc ucugcuaucg gucugaccc ugauguuuuc 6480 uggaccguca uaggggcuca acucgagggg agaagaaca cguauugacgu ggacuacagu 6540 gccuuugacu cucacacgg cucuggcucc ucgaggcucc cuuuuucacc 6600 guggacaug guuuuagcccc ugcgcuggga ccguaucuca gaucccuggc ugucucggug 6660 cacgcuuacg gcgagcgucg caucaagou accgguggcc uccccuccgg uugugccgcg 6720 accagccugc ugacacagu gcucaacaau gugaucauca ggcugcucu ggcauugacu 6780 uacaggaau uugaauauuga caugguugau aucaucgccu acggugacga ccucugguu 6840 ggcacggauu acgaucugga cuucaaugag guggcacgac gcgcugccaa guugggguau 6900 aagaugacuc cugccaacaa ggguucuguc uucccuccga cuuccucucu uuccgaugcu 6960 guuuuucuaa agcgcaaauu cguccaaaac aacgacggcu uauacaaacc aguuauggau 7020 uuaaagaauu uggaagccau gcucuccuac uucaaaccag gaacacuacu cgagaagcug 7080 caaucuguuu cuauguuggc ucaacauucu ggaaaagaag aauaugauag auugaugcac 7140 cccuucgcug acuacggugc cguaccgagu cacgaguacc ugcaggcaag auggagggcc 7200 uuguucgacu gacccagaua gcccaaggcg cuucggugcu gccggcgauu cugggagaac 7260 ucagucggaa cagaaaaggg aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 7310 <210> 15 <211> 2181 <212> PRT <213> Seneca virus <400> 15 Met Gln Asn Ser His Phe Ser Phe Asp Thr Ala Ser Gly Thr Phe Glu 1 5 10 15 Asp Val Thr Gly Thr Lys Val Lys Ile Val Glu Tyr Pro Arg Ser Val 20 25 30 Asn Asn Gly Val Tyr Asp Ser Ser Thr His Leu Glu Ile Leu Asn Leu 35 40 45 Gln Gly Glu Ile Glu Ile Leu Arg Ser Phe Asn Glu Tyr Gln Ile Arg 50 55 60 Ala Ala Lys Gln Gln Leu Gly Leu Asp Ile Val Tyr Glu Leu Gln Gly 65 70 75 80 Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly Asn Asn 85 90 95 Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn Ser Val 100 105 110 Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly Asn Ser 115 120 125 Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile Leu Asn 130 135 140 Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu Asn Ser 145 150 155 160 Ala Asp Arg Val Thr Thr Gln Thr Ala Gly Asn Thr Ala Ile Asn Thr 165 170 175 Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro Thr Lys 180 185 190 Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr Phe Thr 195 200 205 Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr Lys Ala 210 215 220 Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala Phe Leu 225 230 235 240 Ser Arg Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr Leu His 245 250 255 Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln Cys Asn 260 265 270 Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val Pro Glu 275 280 285 Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu Gln Glu 290 295 300 Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg Thr Gly 305 310 315 320 Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro Pro Asn 325 330 335 Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr Val Phe 340 345 350 Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp Ile Asn 355 360 365 Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr Gln Asn 370 375 380 Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr Lys Glu 385 390 395 400 Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro Thr Ser 405 410 415 Pro Tyr Phe Asn Gly Leu Arg Asn Arg Tyr Thr Ala Gly Thr Asp Glu 420 425 430 Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu Met Phe 435 440 445 Leu Ser Thr Leu Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn Val Arg 450 455 460 Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu Leu Gln 465 470 475 480 Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Glu Arg Val Pro Glu Pro 485 490 495 Val Pro Ala Ser Asp Thr Tyr Val Pro Tyr Val Ala Val Pro Thr Gln 500 505 510 Phe Asp Asp Arg Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser Asp Pro 515 520 525 Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe Ala Asn 530 535 540 Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro Met Met 545 550 555 560 Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly Thr Gln 565 570 575 Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile Trp Asp 580 585 590 Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Val Pro Tyr Ile Ser Pro 595 600 605 Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr Ser Ala 610 615 620 Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu Pro Pro 625 630 635 640 Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala Gly Glu 645 650 655 Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr Val Phe 660 665 670 His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly Asn Thr 675 680 685 Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn His Thr 690 695 700 Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val Ile Lys 705 710 715 720 Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr Gln Glu 725 730 735 Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro Arg Pro 740 745 750 Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Ala Asn Pro 755 760 765 Ser Gly Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn Phe Tyr 770 775 780 Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val Thr Val 785 790 795 800 Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe Pro Ser 805 810 815 Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu His Val 820 825 830 Pro Phe His Phe Thr Gly Arg Thr Pro Arg Ala Phe Ala Ser Lys Gly 835 840 845 Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser Val Leu 850 855 860 Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr Arg Gly 865 870 875 880 Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Val Pro Arg 885 890 895 Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val Tyr Ile 900 905 910 Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro Phe Arg 915 920 925 Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Ile Glu Thr Asn 930 935 940 Pro Gly Pro Ala Ser Asp Asn Pro Ile Leu Glu Phe Leu Glu Ala Glu 945 950 955 960 Asn Asp Leu Val Thr Leu Ala Ser Leu Trp Lys Met Val His Ser Val 965 970 975 Gln Gln Thr Trp Arg Lys Tyr Val Lys Asn Asp Asp Phe Trp Pro Asn 980 985 990 Leu Leu Ser Glu Leu Val Gly Glu Gly Ser Val Ala Leu Ala Ala Thr 995 1000 1005 Leu Ser Asn Gln Ala Ser Val Lys Ala Leu Leu Gly Leu His Phe 1010 1015 1020 Leu Ser Arg Gly Leu Asn Tyr Thr Asp Phe Tyr Ser Leu Leu Ile 1025 1030 1035 Glu Lys Cys Ser Ser Phe Phe Thr Val Glu Pro Pro Pro Pro Pro 1040 1045 1050 Ala Glu Asn Leu Met Thr Lys Pro Ser Val Lys Ser Lys Phe Arg 1055 1060 1065 Lys Leu Phe Lys Met Gln Gly Pro Met Asp Lys Val Lys Asp Trp 1070 1075 1080 Asn Gln Ile Ala Ala Gly Leu Lys Asn Phe Gln Phe Val Arg Asp 1085 1090 1095 Leu Val Lys Glu Val Val Asp Trp Leu Gln Ala Trp Ile Asn Lys 1100 1105 1110 Glu Lys Ala Ser Pro Val Leu Gln Tyr Gln Leu Glu Met Lys Lys 1115 1120 1125 Leu Gly Pro Val Ala Leu Ala His Asp Ala Phe Met Ala Gly Ser 1130 1135 1140 Gly Pro Pro Leu Ser Asp Asp Gln Ile Glu Tyr Leu Gln Asn Leu 1145 1150 1155 Lys Ser Leu Ala Leu Thr Leu Gly Lys Thr Asn Leu Ala Gln Ser 1160 1165 1170 Leu Thr Thr Met Ile Asn Ala Lys Gln Ser Ser Ala Gln Arg Val 1175 1180 1185 Glu Pro Val Val Val Val Leu Arg Gly Lys Pro Gly Cys Gly Lys 1190 1195 1200 Ser Leu Ala Ser Thr Leu Ile Ala Gln Ala Val Ser Lys Arg Leu 1205 1210 1215 Tyr Gly Ser Gln Ser Val Tyr Ser Leu Pro Pro Asp Pro Asp Phe 1220 1225 1230 Phe Asp Gly Tyr Lys Gly Gln Phe Val Thr Leu Met Asp Asp Leu 1235 1240 1245 Gly Gln Asn Pro Asp Gly Gln Asp Phe Ser Thr Phe Cys Gln Met 1250 1255 1260 Val Ser Thr Ala Gln Phe Leu Pro Asn Met Ala Asp Leu Ala Glu 1265 1270 1275 Lys Gly Arg Pro Phe Thr Ser Asn Leu Ile Ile Ala Thr Thr Asn 1280 1285 1290 Leu Pro His Phe Ser Pro Val Thr Ile Ala Asp Pro Ser Ala Val 1295 1300 1305 Ser Arg Arg Ile Asn Tyr Asp Leu Thr Leu Glu Val Ser Glu Ala 1310 1315 1320 Tyr Lys Lys His Thr Arg Leu Asn Phe Asp Leu Ala Phe Arg Arg 1325 1330 1335 Thr Asp Ala Pro Pro Ile Tyr Pro Phe Ala Ala His Val Pro Phe 1340 1345 1350 Val Asp Val Ala Val Arg Phe Lys Asn Gly His Gln Asn Phe Asn 1355 1360 1365 Leu Leu Glu Leu Val Asp Ser Ile Cys Thr Asp Ile Arg Ala Lys 1370 1375 1380 Gln Gln Gly Ala Arg Asn Met Gln Thr Leu Val Leu Gln Ser Pro 1385 1390 1395 Asn Glu Asn Asp Asp Thr Pro Val Asp Glu Ala Leu Gly Arg Val 1400 1405 1410 Leu Ser Pro Ala Ala Val Asp Glu Ala Leu Val Asp Leu Thr Pro 1415 1420 1425 Glu Ala Asp Pro Val Gly Arg Leu Ala Ile Leu Ala Lys Leu Gly 1430 1435 1440 Leu Ala Leu Ala Ala Val Thr Pro Gly Leu Ile Ile Leu Ala Val 1445 1450 1455 Gly Leu Tyr Arg Tyr Phe Ser Gly Ser Asp Ala Asp Gln Glu Glu 1460 1465 1470 Thr Glu Ser Glu Gly Ser Val Lys Ala Pro Arg Ser Glu Asn Ala 1475 1480 1485 Tyr Asp Gly Pro Lys Lys Asn Ser Lys Pro Pro Gly Ala Leu Ser 1490 1495 1500 Leu Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala 1505 1510 1515 Ala Val Ala Lys Lys Val Val Val Pro Ile Thr Phe Met Val Pro 1520 1525 1530 Asn Arg Pro Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Thr Gly 1535 1540 1545 Arg Thr Phe Leu Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp 1550 1555 1560 Thr Ser Phe Thr Ile Arg Gly Glu Val His Thr Arg Asp Glu Pro 1565 1570 1575 Phe Gln Thr Val His Phe Thr His His Gly Ile Pro Thr Asp Leu 1580 1585 1590 Met Met Val Arg Leu Gly Pro Gly Asn Ser Phe Pro Asn Asn Leu 1595 1600 1605 Asp Lys Phe Gly Leu Asp Gln Met Pro Ala Arg Asn Ser Arg Val 1610 1615 1620 Val Gly Val Ser Ser Ser Tyr Gly Asn Phe Phe Phe Ser Gly Asn 1625 1630 1635 Phe Leu Gly Phe Val Asp Ser Ile Thr Ser Glu Gln Gly Thr Tyr 1640 1645 1650 Ala Arg Leu Phe Arg Tyr Arg Val Thr Thr Tyr Lys Gly Trp Cys 1655 1660 1665 Gly Ser Ala Leu Val Cys Glu Ala Gly Gly Val Arg Arg Ile Ile 1670 1675 1680 Gly Leu His Ser Ala Gly Ala Ala Gly Ile Gly Ala Gly Thr Tyr 1685 1690 1695 Ile Ser Lys Leu Gly Leu Ile Lys Ala Leu Lys His Leu Gly Glu 1700 1705 1710 Pro Leu Ala Thr Met Gln Gly Leu Met Thr Glu Leu Glu Pro Gly 1715 1720 1725 Ile Thr Val His Val Pro Arg Lys Ser Lys Leu Arg Lys Thr Thr 1730 1735 1740 Ala His Ala Val Tyr Lys Pro Glu Phe Glu Pro Ala Val Leu Ser 1745 1750 1755 Lys Phe Asp Pro Arg Leu Asn Lys Asp Val Asp Leu Asp Glu Val 1760 1765 1770 Ile Trp Ser Lys His Thr Ala Asn Val Pro Tyr Gln Pro Pro Leu 1775 1780 1785 Phe Tyr Thr Tyr Met Ser Glu Tyr Ala His Arg Val Phe Ser Phe 1790 1795 1800 Leu Gly Lys Asp Asn Asp Ile Leu Thr Val Lys Glu Ala Ile Leu 1805 1810 1815 Gly Ile Pro Gly Leu Asp Pro Met Asp Pro His Thr Ala Pro Gly 1820 1825 1830 Leu Pro Tyr Ala Ile Asn Gly Leu Arg Arg Thr Asp Leu Val Asp 1835 1840 1845 Phe Val Asn Gly Thr Val Asp Ala Ala Leu Ala Val Gln Ile Gln 1850 1855 1860 Lys Phe Leu Asp Gly Asp Tyr Ser Asp His Val Phe Gln Thr Phe 1865 1870 1875 Leu Lys Asp Glu Ile Arg Pro Ser Glu Lys Val Arg Ala Gly Lys 1880 1885 1890 Thr Arg Ile Val Asp Val Pro Ser Leu Ala His Cys Ile Val Gly 1895 1900 1905 Arg Met Leu Leu Gly Arg Phe Ala Ala Lys Phe Gln Ser His Pro 1910 1915 1920 Gly Phe Leu Leu Gly Ser Ala Ile Gly Ser Asp Pro Asp Val Phe 1925 1930 1935 Trp Thr Val Ile Gly Ala Gln Leu Glu Gly Arg Lys Asn Thr Tyr 1940 1945 1950 Asp Val Asp Tyr Ser Ala Phe Asp Ser Ser His Gly Thr Gly Ser 1955 1960 1965 Phe Glu Ala Leu Ile Ser His Phe Phe Thr Val Asp Asn Gly Phe 1970 1975 1980 Ser Pro Ala Leu Gly Pro Tyr Leu Arg Ser Leu Ala Val Ser Val 1985 1990 1995 His Ala Tyr Gly Glu Arg Arg Ile Lys Ile Thr Gly Gly Leu Pro 2000 2005 2010 Ser Gly Cys Ala Ala Thr Ser Leu Leu Asn Thr Val Leu Asn Asn 2015 2020 2025 Val Ile Ile Arg Thr Ala Leu Ala Leu Thr Tyr Lys Glu Phe Glu 2030 2035 2040 Tyr Asp Met Val Asp Ile Ile Ala Tyr Gly Asp Asp Leu Leu Val 2045 2050 2055 Gly Thr Asp Tyr Asp Leu Asp Phe Asn Glu Val Ala Arg Arg Ala 2060 2065 2070 Ala Lys Leu Gly Tyr Lys Met Thr Pro Ala Asn Lys Gly Ser Val 2075 2080 2085 Phe Pro Pro Thr Ser Ser Leu Ser Asp Ala Val Phe Leu Lys Arg 2090 2095 2100 Lys Phe Val Gln Asn Asn Asp Gly Leu Tyr Lys Pro Val Met Asp 2105 2110 2115 Leu Lys Asn Leu Glu Ala Met Leu Ser Tyr Phe Lys Pro Gly Thr 2120 2125 2130 Leu Leu Glu Lys Leu Gln Ser Val Ser Met Leu Ala Gln His Ser 2135 2140 2145 Gly Lys Glu Glu Tyr Asp Arg Leu Met His Pro Phe Ala Asp Tyr 2150 2155 2160 Gly Ala Val Pro Ser His Glu Tyr Leu Gln Ala Arg Trp Arg Ala 2165 2170 2175 Leu Phe Asp 2180 <210> 16 <211> 2181 <212> PRT <213> Seneca virus <400> 16 Met Gln Asn Ser His Phe Ser Phe Asp Thr Ala Ser Gly Thr Phe Glu 1 5 10 15 Asp Val Thr Gly Thr Lys Val Lys Ile Val Glu Tyr Pro Arg Ser Val 20 25 30 Asn Asn Gly Val Tyr Asp Ser Ser Thr His Leu Glu Ile Leu Asn Leu 35 40 45 Gln Gly Glu Ile Glu Ile Leu Arg Ser Phe Asn Glu Tyr Gln Ile Arg 50 55 60 Ala Ala Lys Gln Gln Leu Gly Leu Asp Ile Val Tyr Glu Leu Gln Gly 65 70 75 80 Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly Asn Asn 85 90 95 Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn Ser Val 100 105 110 Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly Asn Ser 115 120 125 Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile Leu Asn 130 135 140 Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu Asn Ser 145 150 155 160 Ala Asp Arg Val Thr Thr Gln Thr Ala Gly Asn Thr Ala Ile Asn Thr 165 170 175 Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro Thr Lys 180 185 190 Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr Phe Thr 195 200 205 Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr Lys Ala 210 215 220 Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala Phe Leu 225 230 235 240 Ser Arg Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr Leu His 245 250 255 Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln Cys Asn 260 265 270 Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val Pro Glu 275 280 285 Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu Gln Glu 290 295 300 Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg Thr Gly 305 310 315 320 Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro Pro Asn 325 330 335 Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr Val Phe 340 345 350 Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp Ile Asn 355 360 365 Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr Gln Asn 370 375 380 Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr Lys Glu 385 390 395 400 Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro Thr Ser 405 410 415 Pro Tyr Phe Asn Gly Leu Arg Asn Arg Tyr Thr Ala Gly Thr Asp Glu 420 425 430 Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu Met Phe 435 440 445 Leu Ser Thr Leu Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn Val Arg 450 455 460 Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu Leu Gln 465 470 475 480 Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Glu Arg Val Pro Glu Pro 485 490 495 Val Pro Ala Ser Asp Thr Tyr Val Pro Tyr Val Ala Val Pro Thr Gln 500 505 510 Phe Asp Asp Arg Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser Asp Pro 515 520 525 Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe Ala Asn 530 535 540 Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro Met Met 545 550 555 560 Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly Thr Gln 565 570 575 Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile Trp Asp 580 585 590 Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Val Pro Tyr Ile Ser Pro 595 600 605 Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr Ser Ala 610 615 620 Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu Pro Pro 625 630 635 640 Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala Gly Glu 645 650 655 Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr Val Phe 660 665 670 His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly Asn Thr 675 680 685 Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn His Thr 690 695 700 Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val Ile Lys 705 710 715 720 Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr Gln Glu 725 730 735 Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro Arg Pro 740 745 750 Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Ala Asn Pro 755 760 765 Ser Gly Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn Phe Tyr 770 775 780 Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val Thr Val 785 790 795 800 Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe Pro Ser 805 810 815 Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu His Val 820 825 830 Pro Phe His Phe Thr Gly Arg Thr Pro Arg Ala Phe Ala Ser Lys Gly 835 840 845 Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser Val Leu 850 855 860 Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr Arg Gly 865 870 875 880 Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Val Pro Arg 885 890 895 Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val Tyr Ile 900 905 910 Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro Phe Arg 915 920 925 Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Ile Glu Thr Asn 930 935 940 Pro Gly Pro Ala Ser Asp Asn Pro Ile Leu Glu Phe Leu Glu Ala Glu 945 950 955 960 Asn Asp Leu Val Thr Leu Ala Ser Leu Trp Lys Met Val His Ser Val 965 970 975 Gln Gln Thr Trp Arg Lys Tyr Val Lys Asn Asp Asp Phe Trp Pro Asn 980 985 990 Leu Leu Ser Glu Leu Val Gly Glu Gly Ser Val Ala Leu Ala Ala Thr 995 1000 1005 Leu Ser Asn Gln Ala Ser Val Lys Ala Leu Leu Gly Leu His Phe 1010 1015 1020 Leu Ser Arg Gly Leu Asn Tyr Thr Asp Phe Tyr Ser Leu Leu Ile 1025 1030 1035 Glu Lys Cys Ser Ser Phe Phe Thr Val Glu Pro Pro Pro Pro Pro 1040 1045 1050 Ala Glu Asn Leu Met Thr Lys Pro Ser Val Lys Ser Lys Phe Arg 1055 1060 1065 Lys Leu Phe Lys Met Gln Gly Pro Met Asp Lys Val Lys Asp Trp 1070 1075 1080 Asn Gln Ile Ala Ala Gly Leu Lys Asn Phe Gln Phe Val Arg Asp 1085 1090 1095 Leu Val Lys Glu Val Val Asp Trp Leu Gln Ala Trp Ile Asn Lys 1100 1105 1110 Glu Lys Ala Ser Pro Val Leu Gln Tyr Gln Leu Glu Met Lys Lys 1115 1120 1125 Leu Gly Pro Val Ala Leu Ala His Asp Ala Phe Met Ala Gly Ser 1130 1135 1140 Gly Pro Pro Leu Ser Asp Asp Gln Ile Glu Tyr Leu Gln Asn Leu 1145 1150 1155 Lys Ser Leu Ala Leu Thr Leu Gly Lys Thr Asn Leu Ala Gln Ser 1160 1165 1170 Leu Thr Thr Met Ile Asn Ala Lys Gln Ser Ser Ala Gln Arg Val 1175 1180 1185 Glu Pro Val Val Val Val Leu Arg Gly Lys Pro Gly Cys Gly Lys 1190 1195 1200 Ser Leu Ala Ser Thr Leu Ile Ala Gln Ala Val Ser Lys Arg Leu 1205 1210 1215 Tyr Gly Ser Gln Ser Val Tyr Ser Leu Pro Pro Asp Pro Asp Phe 1220 1225 1230 Phe Asp Gly Tyr Lys Gly Gln Phe Val Thr Leu Met Asp Asp Leu 1235 1240 1245 Gly Gln Asn Pro Asp Gly Gln Asp Phe Ser Thr Phe Cys Gln Met 1250 1255 1260 Val Ser Thr Ala Gln Phe Leu Pro Asn Met Ala Asp Leu Ala Glu 1265 1270 1275 Lys Gly Arg Pro Phe Thr Ser Asn Leu Ile Ile Ala Thr Thr Asn 1280 1285 1290 Leu Pro His Phe Ser Pro Val Thr Ile Ala Asp Pro Ser Ala Val 1295 1300 1305 Ser Arg Arg Ile Asn Tyr Asp Leu Thr Leu Glu Val Ser Glu Ala 1310 1315 1320 Tyr Lys Lys His Thr Arg Leu Asn Phe Asp Leu Ala Phe Arg Arg 1325 1330 1335 Thr Asp Ala Pro Pro Ile Tyr Pro Phe Ala Ala His Val Pro Phe 1340 1345 1350 Val Asp Val Ala Val Arg Phe Lys Asn Gly His Gln Asn Phe Asn 1355 1360 1365 Leu Leu Glu Leu Val Asp Ser Ile Cys Thr Asp Ile Arg Ala Lys 1370 1375 1380 Gln Gln Gly Ala Arg Asn Met Gln Thr Leu Val Leu Gln Ser Pro 1385 1390 1395 Asn Glu Asn Asp Asp Thr Pro Val Asp Glu Ala Leu Gly Arg Val 1400 1405 1410 Leu Ser Pro Ala Ala Val Asp Glu Ala Leu Val Asp Leu Thr Pro 1415 1420 1425 Glu Ala Asp Pro Val Gly Arg Leu Ala Ile Leu Ala Lys Leu Gly 1430 1435 1440 Leu Ala Leu Ala Ala Val Thr Pro Gly Leu Ile Ile Leu Ala Val 1445 1450 1455 Gly Leu Tyr Arg Tyr Phe Ser Gly Ser Asp Ala Asp Gln Glu Glu 1460 1465 1470 Thr Glu Ser Glu Gly Ser Val Lys Ala Pro Arg Ser Glu Asn Ala 1475 1480 1485 Tyr Asp Gly Pro Lys Lys Asn Ser Lys Pro Pro Gly Ala Leu Ser 1490 1495 1500 Leu Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala 1505 1510 1515 Ala Val Ala Lys Lys Val Val Val Pro Ile Thr Phe Met Val Pro 1520 1525 1530 Asn Arg Pro Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Thr Gly 1535 1540 1545 Arg Thr Phe Leu Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp 1550 1555 1560 Thr Ser Phe Thr Ile Arg Gly Glu Val His Thr Arg Asp Glu Pro 1565 1570 1575 Phe Gln Thr Val His Phe Thr His His Gly Ile Pro Thr Asp Leu 1580 1585 1590 Met Met Val Arg Leu Gly Pro Gly Asn Ser Phe Pro Asn Asn Leu 1595 1600 1605 Asp Lys Phe Gly Leu Asp Gln Met Pro Ala Arg Asn Ser Arg Val 1610 1615 1620 Val Gly Val Ser Ser Ser Tyr Gly Asn Phe Phe Phe Ser Gly Asn 1625 1630 1635 Phe Leu Gly Phe Val Asp Ser Ile Thr Ser Glu Gln Gly Thr Tyr 1640 1645 1650 Ala Arg Leu Phe Arg Tyr Arg Val Thr Thr Tyr Lys Gly Trp Cys 1655 1660 1665 Gly Ser Ala Leu Val Cys Glu Ala Gly Gly Val Arg Arg Ile Ile 1670 1675 1680 Gly Leu His Ser Ala Gly Ala Ala Gly Ile Gly Ala Gly Thr Tyr 1685 1690 1695 Ile Ser Lys Leu Gly Leu Ile Lys Ala Leu Lys His Leu Gly Glu 1700 1705 1710 Pro Leu Ala Thr Met Gln Gly Leu Met Thr Glu Leu Glu Pro Gly 1715 1720 1725 Ile Thr Val His Val Pro Arg Lys Ser Lys Leu Arg Lys Thr Thr 1730 1735 1740 Ala His Ala Val Tyr Lys Pro Glu Phe Glu Pro Ala Val Leu Ser 1745 1750 1755 Lys Phe Asp Pro Arg Leu Asn Lys Asp Val Asp Leu Asp Glu Val 1760 1765 1770 Ile Trp Ser Lys His Thr Ala Asn Val Pro Tyr Gln Pro Pro Leu 1775 1780 1785 Phe Tyr Thr Tyr Met Ser Glu Tyr Ala His Arg Val Phe Ser Phe 1790 1795 1800 Leu Gly Lys Asp Asn Asp Ile Leu Thr Val Lys Glu Ala Ile Leu 1805 1810 1815 Gly Ile Pro Gly Leu Asp Pro Met Asp Pro His Thr Ala Pro Gly 1820 1825 1830 Leu Pro Tyr Ala Ile Asn Gly Leu Arg Arg Thr Asp Leu Val Asp 1835 1840 1845 Phe Val Asn Gly Thr Val Asp Ala Ala Leu Ala Val Gln Ile Gln 1850 1855 1860 Lys Phe Leu Asp Gly Asp Tyr Ser Asp His Val Phe Gln Thr Phe 1865 1870 1875 Leu Lys Asp Glu Ile Arg Pro Ser Glu Lys Val Arg Ala Gly Lys 1880 1885 1890 Thr Arg Ile Val Asp Val Pro Ser Leu Ala His Cys Ile Val Gly 1895 1900 1905 Arg Met Leu Leu Gly Arg Phe Ala Ala Lys Phe Gln Ser His Pro 1910 1915 1920 Gly Phe Leu Leu Gly Ser Ala Ile Gly Ser Asp Pro Asp Val Phe 1925 1930 1935 Trp Thr Val Ile Gly Ala Gln Leu Glu Gly Arg Lys Asn Thr Tyr 1940 1945 1950 Asp Val Asp Tyr Ser Ala Phe Asp Ser Ser His Gly Thr Gly Ser 1955 1960 1965 Phe Glu Ala Leu Ile Ser His Phe Phe Thr Val Asp Asn Gly Phe 1970 1975 1980 Ser Pro Ala Leu Gly Pro Tyr Leu Arg Ser Leu Ala Val Ser Val 1985 1990 1995 His Ala Tyr Gly Glu Arg Arg Ile Lys Ile Thr Gly Gly Leu Pro 2000 2005 2010 Ser Gly Cys Ala Ala Thr Ser Leu Leu Asn Thr Val Leu Asn Asn 2015 2020 2025 Val Ile Ile Arg Thr Ala Leu Ala Leu Thr Tyr Lys Glu Phe Glu 2030 2035 2040 Tyr Asp Met Val Asp Ile Ile Ala Tyr Gly Asp Asp Leu Leu Val 2045 2050 2055 Gly Thr Asp Tyr Asp Leu Asp Phe Asn Glu Val Ala Arg Arg Ala 2060 2065 2070 Ala Lys Leu Gly Tyr Lys Met Thr Pro Ala Asn Lys Gly Ser Val 2075 2080 2085 Phe Pro Pro Thr Ser Ser Leu Ser Asp Ala Val Phe Leu Lys Arg 2090 2095 2100 Lys Phe Val Gln Asn Asn Asp Gly Leu Tyr Lys Pro Val Met Asp 2105 2110 2115 Leu Lys Asn Leu Glu Ala Met Leu Ser Tyr Phe Lys Pro Gly Thr 2120 2125 2130 Leu Leu Glu Lys Leu Gln Ser Val Ser Met Leu Ala Gln His Ser 2135 2140 2145 Gly Lys Glu Glu Tyr Asp Arg Leu Met His Pro Phe Ala Asp Tyr 2150 2155 2160 Gly Ala Val Pro Ser His Glu Tyr Leu Gln Ala Arg Trp Arg Ala 2165 2170 2175 Leu Phe Asp 2180 <210> 17 <211> 2181 <212> PRT <213> Seneca virus <400> 17 Met Gln Asn Ser His Phe Ser Phe Asp Thr Ala Ser Gly Thr Phe Glu 1 5 10 15 Asp Val Thr Gly Thr Lys Val Lys Ile Val Glu Tyr Pro Arg Ser Val 20 25 30 Asn Asn Gly Val Tyr Asp Ser Ser Thr His Leu Glu Ile Leu Asn Leu 35 40 45 Gln Gly Glu Ile Glu Ile Leu Arg Ser Phe Asn Glu Tyr Gln Ile Arg 50 55 60 Ala Ala Lys Gln Gln Leu Gly Leu Asp Ile Val Tyr Glu Leu Gln Gly 65 70 75 80 Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly Asn Asn 85 90 95 Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn Ser Val 100 105 110 Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly Asn Ser 115 120 125 Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile Leu Asn 130 135 140 Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu Asn Ser 145 150 155 160 Ala Asp Arg Val Thr Thr Gln Thr Ala Gly Asn Thr Ala Ile Asn Thr 165 170 175 Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro Thr Lys 180 185 190 Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr Phe Thr 195 200 205 Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr Lys Ala 210 215 220 Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala Phe Leu 225 230 235 240 Ser Arg Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr Leu His 245 250 255 Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln Cys Asn 260 265 270 Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val Pro Glu 275 280 285 Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu Gln Glu 290 295 300 Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg Thr Gly 305 310 315 320 Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro Pro Asn 325 330 335 Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr Val Phe 340 345 350 Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp Ile Asn 355 360 365 Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr Gln Asn 370 375 380 Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr Lys Glu 385 390 395 400 Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro Thr Ser 405 410 415 Pro Tyr Phe Asn Gly Leu Arg Asn Arg Tyr Thr Ala Gly Thr Asp Glu 420 425 430 Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu Met Phe 435 440 445 Leu Ser Thr Leu Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn Val Arg 450 455 460 Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu Leu Gln 465 470 475 480 Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Glu Arg Val Pro Glu Pro 485 490 495 Val Pro Ala Ser Asp Thr Tyr Val Pro Tyr Val Ala Val Pro Thr Gln 500 505 510 Phe Asp Asp Arg Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser Asp Pro 515 520 525 Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe Ala Asn 530 535 540 Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro Met Met 545 550 555 560 Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly Thr Gln 565 570 575 Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile Trp Asp 580 585 590 Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Val Pro Tyr Ile Ser Pro 595 600 605 Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr Ser Ala 610 615 620 Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu Pro Pro 625 630 635 640 Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala Gly Glu 645 650 655 Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr Val Phe 660 665 670 His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly Asn Thr 675 680 685 Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn His Thr 690 695 700 Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val Ile Lys 705 710 715 720 Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr Gln Glu 725 730 735 Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro Arg Pro 740 745 750 Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Ala Asn Pro 755 760 765 Ser Gly Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn Phe Tyr 770 775 780 Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val Thr Val 785 790 795 800 Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe Pro Ser 805 810 815 Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu His Val 820 825 830 Pro Phe His Phe Thr Gly Arg Thr Pro Arg Ala Phe Ala Ser Lys Gly 835 840 845 Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser Val Leu 850 855 860 Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr Arg Gly 865 870 875 880 Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Val Pro Arg 885 890 895 Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val Tyr Ile 900 905 910 Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro Phe Arg 915 920 925 Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Ile Glu Thr Asn 930 935 940 Pro Gly Pro Ala Ser Asp Asn Pro Ile Leu Glu Phe Leu Glu Ala Glu 945 950 955 960 Asn Asp Leu Val Thr Leu Ala Ser Leu Trp Lys Met Val His Ser Val 965 970 975 Gln Gln Thr Trp Arg Lys Tyr Val Lys Asn Asp Asp Phe Trp Pro Asn 980 985 990 Leu Leu Ser Glu Leu Val Gly Glu Gly Ser Val Ala Leu Ala Ala Thr 995 1000 1005 Leu Ser Asn Gln Ala Ser Val Lys Ala Leu Leu Gly Leu His Phe 1010 1015 1020 Leu Ser Arg Gly Leu Asn Tyr Thr Asp Phe Tyr Ser Leu Leu Ile 1025 1030 1035 Glu Lys Cys Ser Ser Phe Phe Thr Val Glu Pro Pro Pro Pro Pro 1040 1045 1050 Ala Glu Asn Leu Met Thr Lys Pro Ser Val Lys Ser Lys Phe Arg 1055 1060 1065 Lys Leu Phe Lys Met Gln Gly Pro Met Asp Lys Val Lys Asp Trp 1070 1075 1080 Asn Gln Ile Ala Ala Gly Leu Lys Asn Phe Gln Phe Val Arg Asp 1085 1090 1095 Leu Val Lys Glu Val Val Asp Trp Leu Gln Ala Trp Ile Asn Lys 1100 1105 1110 Glu Lys Ala Ser Pro Val Leu Gln Tyr Gln Leu Glu Met Lys Lys 1115 1120 1125 Leu Gly Pro Val Ala Leu Ala His Asp Ala Phe Met Ala Gly Ser 1130 1135 1140 Gly Pro Pro Leu Ser Asp Asp Gln Ile Glu Tyr Leu Gln Asn Leu 1145 1150 1155 Lys Ser Leu Ala Leu Thr Leu Gly Lys Thr Asn Leu Ala Gln Ser 1160 1165 1170 Leu Thr Thr Met Ile Asn Ala Lys Gln Ser Ser Ala Gln Arg Val 1175 1180 1185 Glu Pro Val Val Val Val Leu Arg Gly Lys Pro Gly Cys Gly Lys 1190 1195 1200 Ser Leu Ala Ser Thr Leu Ile Ala Gln Ala Val Ser Lys Arg Leu 1205 1210 1215 Tyr Gly Ser Gln Ser Val Tyr Ser Leu Pro Pro Asp Pro Asp Phe 1220 1225 1230 Phe Asp Gly Tyr Lys Gly Gln Phe Val Thr Leu Met Asp Asp Leu 1235 1240 1245 Gly Gln Asn Pro Asp Gly Gln Asp Phe Ser Thr Phe Cys Gln Met 1250 1255 1260 Val Ser Thr Ala Gln Phe Leu Pro Asn Met Ala Asp Leu Ala Glu 1265 1270 1275 Lys Gly Arg Pro Phe Thr Ser Asn Leu Ile Ile Ala Thr Thr Asn 1280 1285 1290 Leu Pro His Phe Ser Pro Val Thr Ile Ala Asp Pro Ser Ala Val 1295 1300 1305 Ser Arg Arg Ile Asn Tyr Asp Leu Thr Leu Glu Val Ser Glu Ala 1310 1315 1320 Tyr Lys Lys His Thr Arg Leu Asn Phe Asp Leu Ala Phe Arg Arg 1325 1330 1335 Thr Asp Ala Pro Pro Ile Tyr Pro Phe Ala Ala His Val Pro Phe 1340 1345 1350 Val Asp Val Ala Val Arg Phe Lys Asn Gly His Gln Asn Phe Asn 1355 1360 1365 Leu Leu Glu Leu Val Asp Ser Ile Cys Thr Asp Ile Arg Ala Lys 1370 1375 1380 Gln Gln Gly Ala Arg Asn Met Gln Thr Leu Val Leu Gln Ser Pro 1385 1390 1395 Asn Glu Asn Asp Asp Thr Pro Val Asp Glu Ala Leu Gly Arg Val 1400 1405 1410 Leu Ser Pro Ala Ala Val Asp Glu Ala Leu Val Asp Leu Thr Pro 1415 1420 1425 Glu Ala Asp Pro Val Gly Arg Leu Ala Ile Leu Ala Lys Leu Gly 1430 1435 1440 Leu Ala Leu Ala Ala Val Thr Pro Gly Leu Ile Ile Leu Ala Val 1445 1450 1455 Gly Leu Tyr Arg Tyr Phe Ser Gly Ser Asp Ala Asp Gln Glu Glu 1460 1465 1470 Thr Glu Ser Glu Gly Ser Val Lys Ala Pro Arg Ser Glu Asn Ala 1475 1480 1485 Tyr Asp Gly Pro Lys Lys Asn Ser Lys Pro Pro Gly Ala Leu Ser 1490 1495 1500 Leu Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala 1505 1510 1515 Ala Val Ala Lys Lys Val Val Val Pro Ile Thr Phe Met Val Pro 1520 1525 1530 Asn Arg Pro Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Thr Gly 1535 1540 1545 Arg Thr Phe Leu Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp 1550 1555 1560 Thr Ser Phe Thr Ile Arg Gly Glu Val His Thr Arg Asp Glu Pro 1565 1570 1575 Phe Gln Thr Val His Phe Thr His His Gly Ile Pro Thr Asp Leu 1580 1585 1590 Met Met Val Arg Leu Gly Pro Gly Asn Ser Phe Pro Asn Asn Leu 1595 1600 1605 Asp Lys Phe Gly Leu Asp Gln Met Pro Ala Arg Asn Ser Arg Val 1610 1615 1620 Val Gly Val Ser Ser Ser Tyr Gly Asn Phe Phe Phe Ser Gly Asn 1625 1630 1635 Phe Leu Gly Phe Val Asp Ser Ile Thr Ser Glu Gln Gly Thr Tyr 1640 1645 1650 Ala Arg Leu Phe Arg Tyr Arg Val Thr Thr Tyr Lys Gly Trp Cys 1655 1660 1665 Gly Ser Ala Leu Val Cys Glu Ala Gly Gly Val Arg Arg Ile Ile 1670 1675 1680 Gly Leu His Ser Ala Gly Ala Ala Gly Ile Gly Ala Gly Thr Tyr 1685 1690 1695 Ile Ser Lys Leu Gly Leu Ile Lys Ala Leu Lys His Leu Gly Glu 1700 1705 1710 Pro Leu Ala Thr Met Gln Gly Leu Met Thr Glu Leu Glu Pro Gly 1715 1720 1725 Ile Thr Val His Val Pro Arg Lys Ser Lys Leu Arg Lys Thr Thr 1730 1735 1740 Ala His Ala Val Tyr Lys Pro Glu Phe Glu Pro Ala Val Leu Ser 1745 1750 1755 Lys Phe Asp Pro Arg Leu Asn Lys Asp Val Asp Leu Asp Glu Val 1760 1765 1770 Ile Trp Ser Lys His Thr Ala Asn Val Pro Tyr Gln Pro Pro Leu 1775 1780 1785 Phe Tyr Thr Tyr Met Ser Glu Tyr Ala His Arg Val Phe Ser Phe 1790 1795 1800 Leu Gly Lys Asp Asn Asp Ile Leu Thr Val Lys Glu Ala Ile Leu 1805 1810 1815 Gly Ile Pro Gly Leu Asp Pro Met Asp Pro His Thr Ala Pro Gly 1820 1825 1830 Leu Pro Tyr Ala Ile Asn Gly Leu Arg Arg Thr Asp Leu Val Asp 1835 1840 1845 Phe Val Asn Gly Thr Val Asp Ala Ala Leu Ala Val Gln Ile Gln 1850 1855 1860 Lys Phe Leu Asp Gly Asp Tyr Ser Asp His Val Phe Gln Thr Phe 1865 1870 1875 Leu Lys Asp Glu Ile Arg Pro Ser Glu Lys Val Arg Ala Gly Lys 1880 1885 1890 Thr Arg Ile Val Asp Val Pro Ser Leu Ala His Cys Ile Val Gly 1895 1900 1905 Arg Met Leu Leu Gly Arg Phe Ala Ala Lys Phe Gln Ser His Pro 1910 1915 1920 Gly Phe Leu Leu Gly Ser Ala Ile Gly Ser Asp Pro Asp Val Phe 1925 1930 1935 Trp Thr Val Ile Gly Ala Gln Leu Glu Gly Arg Lys Asn Thr Tyr 1940 1945 1950 Asp Val Asp Tyr Ser Ala Phe Asp Ser Ser His Gly Thr Gly Ser 1955 1960 1965 Phe Glu Ala Leu Ile Ser His Phe Phe Thr Val Asp Asn Gly Phe 1970 1975 1980 Ser Pro Ala Leu Gly Pro Tyr Leu Arg Ser Leu Ala Val Ser Val 1985 1990 1995 His Ala Tyr Gly Glu Arg Arg Ile Lys Ile Thr Gly Gly Leu Pro 2000 2005 2010 Ser Gly Cys Ala Ala Thr Ser Leu Leu Asn Thr Val Leu Asn Asn 2015 2020 2025 Val Ile Ile Arg Thr Ala Leu Ala Leu Thr Tyr Lys Glu Phe Glu 2030 2035 2040 Tyr Asp Met Val Asp Ile Ile Ala Tyr Gly Asp Asp Leu Leu Val 2045 2050 2055 Gly Thr Asp Tyr Asp Leu Asp Phe Asn Glu Val Ala Arg Arg Ala 2060 2065 2070 Ala Lys Leu Gly Tyr Lys Met Thr Pro Ala Asn Lys Gly Ser Val 2075 2080 2085 Phe Pro Pro Thr Ser Ser Leu Ser Asp Ala Val Phe Leu Lys Arg 2090 2095 2100 Lys Phe Val Gln Asn Asn Asp Gly Leu Tyr Lys Pro Val Met Asp 2105 2110 2115 Leu Lys Asn Leu Glu Ala Met Leu Ser Tyr Phe Lys Pro Gly Thr 2120 2125 2130 Leu Leu Glu Lys Leu Gln Ser Val Ser Met Leu Ala Gln His Ser 2135 2140 2145 Gly Lys Glu Glu Tyr Asp Arg Leu Met His Pro Phe Ala Asp Tyr 2150 2155 2160 Gly Ala Val Pro Ser His Glu Tyr Leu Gln Ala Arg Trp Arg Ala 2165 2170 2175 Leu Phe Asp 2180 <210> 18 <211> 3308 <212> DNA <213> Seneca virus [[ID=X]]<400> 18 ggatccgcca ccatgggtaa tgttcagaca acctcaaaga atgattttga ttcccgcggc 60<00]2928>ataatggta acatgacctt cattactac gcaacactt accagaattc agtagacttc 120 tcgacctcct cgtcggcgtc aggcgccgga cccgggaact cccggggcgg actagcgggt 180 ctcctcacaa atttcagtgg aatcttgaac cctcttggct acctcaaga tcacaatacc 240 gagaaatgg aaaactctgc tgatcgagtc ataacgcaaa cggcgggcaa cactgccata 300 aacacgcaat catcactggg tgtgttgtgt gcctacgttg agacccgac caatctgac 360 cctccgtcca gcagcacaga tcacccacc accacttta ctgccatcga caggtggtac 420 actggacgcc tcattctg gandaaagct gtaaaaacct tctctttca ggccgtcccg 480 ctccctggag ccttcctgtc taaacaggga ggctcaacg gaggggcctt cacggctacc 540 ctacatagac atttcttaat gaagtgcggg tggcaggtgc aggtccaatg caatttgacg 600 caatccacc aaggtgctct tcttgttgcc atggtccccg aaaccaccct tgatgtcaa 660 cctgacggca aggcaagag cttacaggag ctgaatgaag agcagtggt ggaatgtct 720 gacgattacc ggaccgggaaaacatgcct ttcagtctc ttggcacata ctacggccc 780 cctaactgga cttggggccc caatttcatc aacccctatc aagtaacagt tttcccacac 840 caaattctga acgcgagaac ctctacctcg gtagacataa gtgtcccata catcggggag 900 actcctacac aatcctcaga gacacagaac tcctggaccc tccttgttat ggtgcttgtc 960 cccctggact acaaggaggg agccacaact gacccagaaa ttacattttc tgtaaggcct 1020 acaagtccct acttcaatgg gcttcgtaac cgtttcacga ccgggacgga cgaggaacag 1080 gggcccattc ccacagcacc cagagaaaat tcgcttatgt ttctctcaac catccctgac 1140 gacactgtcc ctgcttacgg gaatgtgcgt acccctcccg tcaattacct ccccggtgaa 1200 ataaccgacc tcttacaact ggcccgtata cccactctca tggcgtttgg gcgggtgtct 1260 gaacccgagc ctgcctcaga cgcatatgtg ccctacgttg ccgttcctgc ccagttcgac 1320 gacaagcctc tcatctcctt cccgatcacc ctttcagatc ctgtctacca gaacaccctg 1380 gtaggcgcca tcagttcgaa cttcgccaac taccgggggt gtatccaaat cactctgaca 1440 ttttgtggac ccatgatggc aagagggaaa ttcctgctct cgtattctcc cccaaatgga 1500 gcacaaccac agaccctttc tgaagctatg cagtgcacat actctatttg ggatataggc 1560 ttgaactcta gttggacctt tgtcatcccc tacatctcgc ccagtgatta ccgtgaaact 1620 cgggctatta ccaactcagt ttatctgct gatggttggt ttagcttgca caagctgacc 1680 aaaattactc taccacctga ctgcccacag agtccctgta ttctcttttt cgcctctgct 1740 ggtgaggatt acaccctccg tctccctgtt gattgtaatc cttcctacgt gttccactcc 1800 accgacaacg ccgagactgg ggtattgag gcaggtaaca ctgacaccga tttctctggt 1860 gaactggcgg ctcctggctc taaccatact aatgtcaaat tcctgtttga ccgatctcga 1920 ctactgaatg taattaaggt actggagaag gacgccgtct tcccccgtcc tttccccaca 1980 gcaacaggtg cacagcagga cgatggttac ttttgtcttc taacaccccg cccaacagtc 2040 gcttccccgac ccgccactcg tttcggcctg tacgtcaacc cgtctgacag tggcgttctc 2100 gctaacactt cactggattt caatttttac agtttggcct gtttcactta ctttagatca 2160 gaccttgaag tcacggtggt ctcactggag ccagatttgg aattcgccgt ggggtggttc 2220 ccctctggca gtgagtacca ggcttctagc tttgtctacg accaactgca tgtaccctac 2280 cactttactg ggcgcactcc ccgcgctttc accagcaagg gtggaaaggt atctttcgtg 2340 ctcccttgga actctgtctc ttccgtgctt cccgtgcgct gggggggcgc ttccaagctt 2400 tcttctgcca cgcggggtct gccggctcat gctgactggg ggaccattta cgcctttatc 2460 ccccgtccta acgagaagaa aagcaccgct gtaaagcacg tggcggtgta cgttcggtac 2520 aagaacgcgc gtgcttggtg ccccagcatg cttccctttc gcagctacaa gcaaaagatg 2580 ctgatgcaat caggcgacat cgagaccaac cctggccctg gttcaggtaa gccccctgga 2640 gcgctctctc ttatggaaat gcaacagccc aacgtggaca tgggctttga ggctgcggtc 2700 gctaagaaag tggtcgtccc cattaccttc atggttccca acagaccttc tggacttaca 2760 cagtccgctc ttcttgtggc cggccggacc ttcctaatca atgagcatac atggtccaac 2820 ccctcctgga ccagcttcac aatccgtggt gaggtgcaca ctcgtgatga gcctttccaa 2880 acggttcatt ttactcacca tggtcttccc acagatctga tgatggtacg tctcggaccg 2940 ggcaactctt tccctaacaa tctagacaag tttggacttg accagatgcc ggcacgtaac 3000 tcccgtgtgg ttggcgtttc ggctagttac ggtaacttct tcttctctgg gaacttcctc 3060 gggtttgttg actccatcac ctctgaccaa ggaacctatg cgagactttt caggtacagg 3120 gtgacgactt acaagggatg gtgcggttcg gccctggtct gtgaggccgg tggtgtccga 3180 cgcatcattg gcatgcattc tgctggtgcc gctggtatcg gcgccgggac ttacatctca 3240 aaattaggac tgatcaaagc ccttaaacac ctcggtgagc ctctggctac aatgcaatga 3300 gcggccgc 3308 <210> 19 <211> 1095 <212> PRT <213> Seneca virus <400> 19 Met Gly Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly 1 5 10 15 Asn Asn Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn 20 25 30 Ser Val Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly 35 40 45 Asn Ser Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile 50 55 60 Leu Asn Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu 65 70 75 80 Asn Ser Ala Asp Arg Val Ile Thr Gln Thr Ala Gly Asn Thr Ala Ile 85 90 95 Asn Thr Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro 100 105 110 Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr 115 120 125 Phe Thr Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr 130 135 140 Lys Ala Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala 145 150 155 160 Phe Leu Ser Lys Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr 165 170 175 Leu His Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln 180 185 190 Cys Asn Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val 195 200 205 Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu 210 215 220 Gln Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg 225 230 235 240 Thr Gly Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro 245 250 255 Pro Asn Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr 260 265 270 Val Phe Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp 275 280 285 Ile Ser Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr 290 295 300 Gln Asn Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr 305 310 315 320 Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro 325 330 335 Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr 340 345 350 Asp Glu Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu 355 360 365 Met Phe Leu Ser Thr Ile Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn 370 375 380 Val Arg Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu 385 390 395 400 Leu Gln Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Gly Arg Val Ser 405 410 415 Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val Pro Tyr Val Ala Val Pro 420 425 430 Ala Gln Phe Asp Asp Lys Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser 435 440 445 Asp Pro Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe 450 455 460 Ala Asn Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro 465 470 475 480 Met Met Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly 485 490 495 Ala Gln Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile 500 505 510 Trp Asp Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Ile Pro Tyr Ile 515 520 525 Ser Pro Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr 530 535 540 Ser Ala Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu 545 550 555 560 Pro Pro Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala 565 570 575 Gly Glu Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr 580 585 590 Val Phe His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly 595 600 605 Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn 610 615 620 His Thr Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val 625 630 635 640 Ile Lys Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr 645 650 655 Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro 660 665 670 Arg Pro Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Val 675 680 685 Asn Pro Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn 690 695 700 Phe Tyr Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val 705 710 715 720 Thr Val Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe 725 730 735 Pro Ser Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu 740 745 750 His Val Pro Tyr His Phe Thr Gly Arg Thr Pro Arg Ala Phe Thr Ser 755 760 765 Lys Gly Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser 770 775 780 Val Leu Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr 785 790 795 800 Arg Gly Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Ile 805 810 815 Pro Arg Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val 820 825 830 Tyr Val Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro 835 840 845 Phe Arg Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Ile Glu 850 855 860 Thr Asn Pro Gly Pro Gly Ser Gly Lys Pro Pro Gly Ala Leu Ser Leu 865 870 875 880 Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala Ala Val 885 890 895 Ala Lys Lys Val Val Val Pro Ile Thr Phe Met Val Pro Asn Arg Pro 900 905 910 Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Ala Gly Arg Thr Phe Leu 915 920 925 Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp Thr Ser Phe Thr Ile 930 935 940 Arg Gly Glu Val His Thr Arg Asp Glu Pro Phe Gln Thr Val His Phe 945 950 955 960 Thr His His Gly Leu Pro Thr Asp Leu Met Met Val Arg Leu Gly Pro 965 970 975 Gly Asn Ser Phe Pro Asn Asn Leu Asp Lys Phe Gly Leu Asp Gln Met 980 985 990 Pro Ala Arg Asn Ser Arg Val Val Gly Val Ser Ala Ser Tyr Gly Asn 995 1000 1005 Phe Phe Phe Ser Gly Asn Phe Leu Gly Phe Val Asp Ser Ile Thr 1010 1015 1020 Ser Asp Gln Gly Thr Tyr Ala Arg Leu Phe Arg Tyr Arg Val Thr 1025 1030 1035 Thr Tyr Lys Gly Trp Cys Gly Ser Ala Leu Val Cys Glu Ala Gly 1040 1045 1050 Gly Val Arg Arg Ile Ile Gly Met His Ser Ala Gly Ala Ala Gly 1055 1060 1065 Ile Gly Ala Gly Thr Tyr Ile Ser Lys Leu Gly Leu Ile Lys Ala 1070 1075 1080 Leu Lys His Leu Gly Glu Pro Leu Ala Thr Met Gln 1085 1090 1095 <210> 20 <211> 3308 <212> DNA <213> Seneca virus <400> 20 ggatccgcca ccatgggcaa cgttcaaact acctccaaga atgattttga ctcccgcggc 60 aacaacggca acatgacttt taactactac gccaatacgt atcaaaactc tgttgatttc 120 tcgacatcga gttccgcttc cggcgcggga cctggaaatt cccgcggtgg actggccggt 180 ctgctcacaa acttttccgg tatcctgaac cccctcggtt atctgaaaga tcacaatacg 240 gaagagatgg agaactctgc cgatcgtgtg attacgcaga cagctggtaa cacggctatc 300 aacactcaat cgtctctggg agtactctgt gcttacgtcg aggacccaac aaaatccgac 360 cctccatctt cctcgaccga tcaacctacg actaccttca cggccataga caggtggtat 420 acaggtcgcc tgaattcgtg gacaaaggct gttaaaacct ttagcttcca ggctgtgcca 480 ttgcccggtg ctttcctttc ccgccagggc ggcctcaacg gtggagcttt tactgctaca 540 ctgcacagac actttctgat gaaatgcggt tggcaggtgc aggtacagtg caatttgact 600 caattccacc aaggtgcact tctcgttgct atggttcccg agactacgct tgacgttaag 660 cctgacggca aggccaagtc tctccaagag ctgaatgagg aacagtgggt tgagatgagc 720 gatgactata gaaccggtaa aaacatgcca ttccagtccc tgggcacata ctacagaccc 780 ccaaattgga cttggggccc gaactttatc aatccgtatc aggtcaccgt gtttccacac 840 caaatcctga atgccaggac tagtaccagc gtcgatatta gcgtgcccta tatcggcgaa 900 acgccaaccc aatcctccga aactcagaac agctggacgc ttctggtaat ggtgttggta 960 cccttggatt ataaggaggg agccactacc gaccctgaaa tcactttttc ggtaaggccc 1020 actagccctt acttcaacgg actgcgcaac cgtttcacca ccggcactga tgaggagcaa 1080 ggccctatcc ctactgcccc cagagaaaat agcctgatgt ttctgagcac aattcctgac 1140 gacaccgtac ctgcctatgg taacgttcgt acgcctcccg taaactacct gcctggtgaa 1200 attacggacc tcttgcagct ggcccgcatc cctacactga tggccttcgg tcgtgtcagt 1260 gagcccgaac cagccagcga cgcttacgtt ccatatgttg cggtacctgc tcagttcgac 1320 gacaagccgt tgataagttt cccaatcact ttgagcgacc ctgtatacca gaacactttg 1380 gttggagcta tctctcgaa ctttgcgaac taccgtggtt gtattcaaat cacccttaca 1440 ttctgcggcc cgatgatggc tcgtggcaag ttcctgttga gctactcccc ccccaacgga 1500 gcacaaccac agacactgtc cgaggccatg cagtgcacat actccatctg ggatatcggt 1560 ctcaacagct catggacctt tgtaatacca tacatctcgc catccgatta tagagagacc 1620 cgcgccatca ccaacagtgt gtactcagcc gatggatggt tcagtctcca taagcttaca 1680 aagatcactc tgccccccga ctgcccacaa tcaccctgca tattgttctt tgcctcagct 1740 ggtgaagatt acacgcttcg tttgcccgtc gactgtaacc cgtcttacgt gttccactcc 1800 acggacaatg ctgagaccgg tgtaattgag gctggtaaca ccgacacgga tttctctggt 1860 gagctggcgg ctcccggttc taaccatacc aacgtgaagt tccttttcga ccgttcacgt 1920 cttttgaacg taatcaaggt cttggaaaag gacgctgtgt tccctcgccc attccccaca 1980 gcaacgggag cacaacaaga cgacggctat ttctgcctgc tgacaccacg tcctactgtg 2040 gctagccgtc cggccacacg ctttggtctc tacgttaacc ctagcgactc tggtgtactg 2100 gctaacacct ccctggactt caatttctac tctctggcct gcttcacgta cttcaggagc 2160 gacctggagg tcactgtggt gtctctggaa ccagatctgg aatttgcggt aggatggttc 2220 ccttcaggaa gcgagtacca ggcatcgtcc tttgtgtacg accaacttca tgttccctac 2280 catttcaccg gtagaacccc acgtgccttc accagtaagg gaggcaaagt ttctttgta 2340 ctgccctgga attctgtcag ttccgttctc ccggtccgct ggggcggagc gtcaaagctc 2400 tcatccgcga cccgcggatt gcccgcacac gcggattggg gaactatcta cgctttcatt 2460 ccacgcccta atgaaaagaa gtctacagca gtgaagcacg tggccgtcta tgtgcgttac 2520 aagaatgcaa gggcgtggtg tccgtctatg ttgccattcc gtagctataa acagaagatg 2580 cttatgcaaa gcggtgatat tgagacgaat cctggacctg gttcaggtaa gccccctgga 2640 gcgctctctc ttatggaaat gcaacagccc aacgtggaca tgggctttga ggctgcggtc 2700 gctaagaaag tggtcgtccc cattacctt atggttccca acagaccttc tggacttaca 2760 cagtccgctc ttcttgtggc cggccggacc ttcctaatca atgagcatac atggtccaac 2820 ccctcctgga ccagcttcac aatccgtggt gaggtgcaca ctcgtgatga gcctttccaa 2880 acggttcatt ttactcacca tggtcttcc acagatctga tgatggtacg tctcggaccg 2940 ggcaactctt tccctaacaa tctagacaag tttggacttg accagatgcc ggcacgtaac 3000 tcccgtgtgg ttggcgtttc ggctagttac ggtaacttct tcttctctgg gaacttctc 3060 gggtttgttg actccatcac ctctgaccaa ggaacctatg cgagactttt caggtacagg 3120 gtgacgactt acaagggatg gtgcggttcg gccctggtct gtgaggccgg tggtgtccga 3180 cgcatcattg gcatgcattc tgctggtgcc gctggtatcg gcgccgggac ttacatctca 3240 aaattaggac tgatcaaagc ccttaaacac ctcggtgagc ctctggctac aatgcaatga 3300 gcggccgc 3308 <210> 21 <211> 1095 <212> PRT <213> Seneca virus <400> 21 Met Gly Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly 1 5 10 15 Asn Asn Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn 20 25 30 Ser Val Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly 35 40 45 Asn Ser Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile 50 55 60 Leu Asn Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu 65 70 75 80 Asn Ser Ala Asp Arg Val Ile Thr Gln Thr Ala Gly Asn Thr Ala Ile 85 90 95 Asn Thr Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro 100 105 110 Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr 115 120 125 Phe Thr Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr 130 135 140 Lys Ala Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala 145 150 155 160 Phe Leu Ser Arg Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr 165 170 175 Leu His Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln 180 185 190 Cys Asn Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val 195 200 205 Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu 210 215 220 Gln Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg 225 230 235 240 Thr Gly Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro 245 250 255 Pro Asn Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr 260 265 270 Val Phe Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp 275 280 285 Ile Ser Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr 290 295 300 Gln Asn Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr 305 310 315 320 Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro 325 330 335 Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr 340 345 350 Asp Glu Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu 355 360 365 Met Phe Leu Ser Thr Ile Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn 370 375 380 Val Arg Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu 385 390 395 400 Leu Gln Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Gly Arg Val Ser 405 410 415 Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val Pro Tyr Val Ala Val Pro 420 425 430 Ala Gln Phe Asp Asp Lys Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser 435 440 445 Asp Pro Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe 450 455 460 Ala Asn Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro 465 470 475 480 Met Met Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly 485 490 495 Ala Gln Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile 500 505 510 Trp Asp Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Ile Pro Tyr Ile 515 520 525 Ser Pro Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr 530 535 540 Ser Ala Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu 545 550 555 560 Pro Pro Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala 565 570 575 Gly Glu Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr 580 585 590 Val Phe His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly 595 600 605 Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn 610 615 620 His Thr Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val 625 630 635 640 Ile Lys Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr 645 650 655 Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro 660 665 670 Arg Pro Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Val 675 680 685 Asn Pro Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn 690 695 700 Phe Tyr Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val 705 710 715 720 Thr Val Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe 725 730 735 Pro Ser Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu 740 745 750 His Val Pro Tyr His Phe Thr Gly Arg Thr Pro Arg Ala Phe Thr Ser 755 760 765 Lys Gly Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser 770 775 780 Val Leu Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr 785 790 795 800 Arg Gly Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Ile 805 810 815 Pro Arg Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val 820 825 830 Tyr Val Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro 835 840 845 Phe Arg Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Ile Glu 850 855 860 Thr Asn Pro Gly Pro Gly Ser Gly Lys Pro Pro Gly Ala Leu Ser Leu 865 870 875 880 Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala Ala Val 885 890 895 Ala Lys Lys Val Val Val Pro Ile Thr Phe Met Val Pro Asn Arg Pro 900 905 910 Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Ala Gly Arg Thr Phe Leu 915 920 925 Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp Thr Ser Phe Thr Ile 930 935 940 Arg Gly Glu Val His Thr Arg Asp Glu Pro Phe Gln Thr Val His Phe 945 950 955 960 Thr His His Gly Leu Pro Thr Asp Leu Met Met Val Arg Leu Gly Pro 965 970 975 Gly Asn Ser Phe Pro Asn Asn Leu Asp Lys Phe Gly Leu Asp Gln Met 980 985 990 Pro Ala Arg Asn Ser Arg Val Val Gly Val Ser Ala Ser Tyr Gly Asn 995 1000 1005 Phe Phe Phe Ser Gly Asn Phe Leu Gly Phe Val Asp Ser Ile Thr 1010 1015 1020 Ser Asp Gln Gly Thr Tyr Ala Arg Leu Phe Arg Tyr Arg Val Thr 1025 1030 1035 Thr Tyr Lys Gly Trp Cys Gly Ser Ala Leu Val Cys Glu Ala Gly 1040 1045 1050 Gly Val Arg Arg Ile Ile Gly Met His Ser Ala Gly Ala Ala Gly 1055 1060 1065 Ile Gly Ala Gly Thr Tyr Ile Ser Lys Leu Gly Leu Ile Lys Ala 1070 1075 1080 Leu Lys His Leu Gly Glu Pro Leu Ala Thr Met Gln 1085 1090 1095 <210> 22 <211> 3308 <212> DNA <213> Seneca virus <400> 22 ggatccgcca ccatgggtaa tgttcagaca acctcaaaga atgattttga ttcccgcggc 60 aataatggta acatgacctt caattactac gcaaacactt accagaattc agtagacttc 120 tcgacctcct cgtcggcgtc aggcgccgga cccgggaact cccggggcgg actagcgggt 180 ctcctcacaa atttcagtgg aatcttgaac cctcttggct acctcaaaga tcacaatacc 240 gaagaaatgg aaaactctgc tgatcgagtc ataacgcaaa cggcgggcaa cactgccata 300 aacacgcaat catcactggg tgtgttgtgt gcctacgttg aagacccgac caaatctgac 360 cctccgtcca gcagcacaga tcaacccacc accactttta ctgccatcga caggtggtac 420 actggacgcc tcaattcttg gacaaaagct gtaaaaaacct tctcttttca ggccgtcccg 480 ctccctggag ccttcctgtc taaacaggga ggcctcaacg gaggggcctt cacggctacc 540 ctacatagac atttcttaat gaagtgcggg tggcaggtgc aggtccaatg caatttgacg 600 caattccacc aaggtgctct tcttgttgcc atggtccccg aaaccaccct tgatgtcaaa 660 cctgacggca aggcaaagag cttacaggag ctgaatgaag agcagtgggt ggaaatgtct 720 gacgattacc ggaccgggaa aaacatgcct tttcagtctc ttggcacata ctatcggccc 780 cctaactgga cttggggccc caatttcatc aacccctatc aagtaacagt tttcccacac 840 caaattctga acgcgagaac ctctacctcg gtagacataa gtgtcccata catcggggag 900 actcctacac aatcctcaga gacacagaac tcctggaccc tccttgttat ggtgcttgtc 960 cccctggact acaaggggg agccacaact gacccagaaa ttacattttc tgtaaggcct 1020 1080 gggcccattc ccacagcacc cagagaaaat tcgcttatgt ttctctcaac catccctgac 1140 gacactgtcc ctgcttacgg gaatgtgcgt acccctcccg tcaattacct ccccggtgaa 1200 ataaccgacc tcttacaact ggcccgtata cccactctca tggcgtttgg gcgggtgtct 1260 gaacccgagc ctgcctcaga cgcatatgtg ccctacgttg ccgttcctgc ccagttcgac 1320 gacaagcctc tcatctcctt cccgatcacc ctttcagatc ctgtctacca gaacaccctg 1380 gtaggcgcca tcagttcgaa cttcgccaac taccggggt gtatccaaat cactctgaca 1440 ttttgtggac ccatgatggc aagagggaaa ttcctgctct cgtattctcc cccaaatgga 1500 gcacaaccac agaccctttc tgaagctatg cagtgcacat actctatttg ggatataggc 1560 ttgaactcta gttggacctt tgtcatcccc tacatctcgc ccagtgatta ccgtgaaact 1620 cgggctatta ccaactcagt ttatctgct gatggttggt ttagcttgca caagctgacc 1680 aaaattactc taccacctga ctgcccacag agtccctgta ttctcttttt cgcctctgct 1740 ggtgaggatt acaccctccg tctccctgtt gattgtaatc cttcctacgt gcctcagggg 1800 gttgacaacg ccgagactgg ggttattgag gcaggtaaca ctgacaccga tttctctggt 1860 gaactggcgg ctcctggctc taaccatact aatgtcaaat tcctgtttga ccgatctcga 1920 ctactgaatg taattaaggt actggagaag gacgccgtct tcccccgtcc tttccccaca 1980 gcaacaggtg cacagcagga cgatggttac ttttgtcttc taacaccccg cccaacagtc 2040 gcttcccgac ccgccactcg tttcggcctg tacgtcaacc cgtctgacag tggcgttctc 2100 gctaacactt cactggattt caatttttac agtttggcct gtttcactta ctttagatca 2160 gaccttgaag tcacggtggt ctcactggag ccagatttgg aattcgccgt ggggtggttc 2220 ccctctggca gtgagtacca ggcttctagc tttgtctacg accaactgca tgtaccctac 2280 cactttactg ggcgcactcc ccgcgctttc accagcaagg gtggaaaggt atctttcgtg 2340 ctcccttgga actctgtctc ttccgtgctt cccgtgcgct gggggggcgc ttccaagctt 2400 tcttctgcca cgcggggtct gccggctcat gctgactggg ggaccattta cgcctttatc 2460 ccccgtccta acgagaagaa aagcaccgct gtaaagcacg tggcggtgta cgttcggtac 2520 aagaacgcgc gtgcttggtg ccccagcatg cttccctttc gcagctacaa gcaaaagatg 2580 ctgatgcaat caggcgacat cgagaccaac cctggccctg gttcaggtaa gccccctgga 2640 gcgctctctc ttatggaaat gcaacagccc aacgtggaca tgggctttga ggctgcggtc 2700 gctaagaaag tggtcgtccc cattaccttc atggttccca acagaccttc tggacttaca 2760 cagtccgctc ttcttgtggc cggccggacc ttcctaatca atgagcatac atggtccaac 2820 ccctcctgga ccagcttcac aatccgtggt gaggtgcaca ctcgtgatga gcctttccaa 2880 acggttcatt ttactcacca tggtcttccc acagatctga tgatggtacg tctcggaccg 2940 ggcaactctt tccctaacaa tctagacaag tttggacttg accagatgcc ggcacgtaac 3000 tcccgtgtgg ttggcgtttc ggctagttac ggtaacttct tcttctctgg gaacttcctc 3060 gggtttgttg actccatcac ctctgaccaa ggaacctatg cgagactttt caggtacagg 3120 gtgacgactt acaagggatg gtgcggttcg gccctggtct gtgaggccgg tggtgtccga 3180 cgcatcattg gcatgcattc tgctggtgcc gctggtatcg gcgccgggac ttacatctca 3240 aaattaggac tgatcaaagc ccttaaacac ctcggtgagc ctctggctac aatgcaatga 3300 gcggccgc 3308 <210> 23 <211> 1095 <212> PRT <213> Seneca virus <400> 23 Met Gly Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly 1 5 10 15 Asn Asn Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn 20 25 30 Ser Val Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly 35 40 45 Asn Ser Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile 50 55 60 Leu Asn Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu ​​​​​​Asn Thr Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro 100 105 110 Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr 115 120 125 Phe Thr Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr 130 135 140 Lys Ala Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala 145 150 155 160 Phe Leu Ser Lys Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr 165 170 175 Leu His Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln 180 185 190 Cys Asn Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val 195 200 205 Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu 210 215 220 Gln Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg 225 230 235 240 Thr Gly Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro 245 250 255 Pro Asn Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr 260 265 270 Val Phe Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp 275 280 285 Ile Ser Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr 290 295 300 Gln Asn Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr 305 310 315 320 Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro 325 330 335 Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr 340 345 350 Asp Glu Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu 355 360 365 Met Phe Leu Ser Thr Ile Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn 370 375 380 Val Arg Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu 385 390 395 400 Leu Gln Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Gly Arg Val Ser 405 410 415 Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val Pro Tyr Val Ala Val Pro 420 425 430 Ala Gln Phe Asp Asp Lys Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser 435 440 445 Asp Pro Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe 450 455 460 Ala Asn Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro 465 470 475 480 Met Met Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly 485 490 495 Ala Gln Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile 500 505 510 Trp Asp Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Ile Pro Tyr Ile 515 520 525 Ser Pro Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr 530 535 540 Ser Ala Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu 545 550 555 560 Pro Pro Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala 565 570 575 Gly Glu Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr 580 585 590 Val Pro Gln Gly Val Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly 595 600 605 Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn 610 615 620 His Thr Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val 625 630 635 640 Ile Lys Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr 645 650 655 Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro 660 665 670 Arg Pro Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Val 675 680 685 Asn Pro Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn 690 695 700 Phe Tyr Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val 705 710 715 720 Thr Val Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe 725 730 735 Pro Ser Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu 740 745 750 His Val Pro Tyr His Phe Thr Gly Arg Thr Pro Arg Ala Phe Thr Ser 755 760 765 Lys Gly Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser 770 775 780 Val Leu Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr 785 790 795 800 Arg Gly Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Ile 805 810 815 Pro Arg Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val 820 825 830 Tyr Val Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro 835 840 845 Phe Arg Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Ile Glu 850 855 860 Thr Asn Pro Gly Pro Gly Ser Gly Lys Pro Pro Gly Ala Leu Ser Leu 865 870 875 880 Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala Ala Val 885 890 895 Ala Lys Lys Val Val Val Pro Ile Thr Phe Met Val Pro Asn Arg Pro 900 905 910 Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Ala Gly Arg Thr Phe Leu 915 920 925 Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp Thr Ser Phe Thr Ile 930 935 940 Arg Gly Glu Val His Thr Arg Asp Glu Pro Phe Gln Thr Val His Phe 945 950 955 960 Thr His His Gly Leu Pro Thr Asp Leu Met Met Val Arg Leu Gly Pro 965 970 975 Gly Asn Ser Phe Pro Asn Asn Leu Asp Lys Phe Gly Leu Asp Gln Met 980 985 990 Pro Ala Arg Asn Ser Arg Val Val Gly Val Ser Ala Ser Tyr Gly Asn 995 1000 1005 Phe Phe Phe Ser Gly Asn Phe Leu Gly Phe Val Asp Ser Ile Thr 1010 1015 1020 Ser Asp Gln Gly Thr Tyr Ala Arg Leu Phe Arg Tyr Arg Val Thr 1025 1030 1035 Thr Tyr Lys Gly Trp Cys Gly Ser Ala Leu Val Cys Glu Ala Gly 1040 1045 1050 Gly Val Arg Arg Ile Ile Gly Met His Ser Ala Gly Ala Ala Gly 1055 1060 1065 Ile Gly Ala Gly Thr Tyr Ile Ser Lys Leu Gly Leu Ile Lys Ala 1070 1075 1080 Leu Lys His Leu Gly Glu Pro Leu Ala Thr Met Gln 1085 1090 1095 <210> 24 <211> 4694 <212> DNA <213> Seneca virus <400> 24 ggatccgcca ccatgggtaa tgttcagaca acctcaaaga atgattttga ttcccgcggc 60 aataatggta acatgacctt caattactac gcaaacactt accagaattc agtagacttc 120 tcgacctcct cgtcggcgtc aggcgccgga cccgggaact cccggggcgg actagcgggt 180 ctcctcacaa atttcagtgg aatcttgaac cctcttggct acctcaaaga tcacaatacc 240 gaagaaatgg aaaactctgc tgatcgagtc ataacgcaaa cggcgggcaa cactgccata 300 aacacgcaat catcactggg tgtgttgtgt gcctacgttg aagacccgac caaatctgac 360 cctccgtcca gcagcacaga tcaacccacc accactttta ctgccatcga caggtggtac 420 actggacgcc tcaattcttg gacaaaagct gtaaaaaacct tctcttttca ggccgtcccg 480 ctccctggag ccttcctgtc taaacaggga ggcctcaacg gaggggcctt cacggctacc 540 ctacatagac atttcttaat gaagtgcggg tggcaggtgc aggtccaatg caatttgacg 600 caattccacc aaggtgctct tcttgttgcc atggtccccg aaaccaccct tgatgtcaaa 660 cctgacggca aggcaaagag cttacaggag ctgaatgaag agcagtgggt ggaaatgtct 720 gacgattacc ggaccgggaa aaacatgcct tttcagtctc ttggcacata ctatcggccc 780 cctaactgga cttggggccc caatttcatc aacccctatc aagtaacagt tttcccacac 840 caaattctga acgcgagaac ctctacctcg gtagacataa gtgtcccata catcggggag 900 actcctacac aatcctcaga gacacagaac tcctggaccc tccttgttat ggtgcttgtc 960 cccctggact acaaggggg agccacaact gacccagaaa ttacattttc tgtaaggcct 1020 acaagtccct acttcaatgg gcttcgtaac cgtttcacga ccgggacgga cgaggaacag 1080 gggcccattc ccacagcacc cagagaaaat tcgcttatgt ttctctcaac catccctgac 1140 gacactgtcc ctgcttacgg gaatgtgcgt acccctcccg tcaattacct ccccggtgaa 1200 ataaccgacc tcttacaact ggcccgtata cccactctca tggcgtttgg gcgggtgtct 1260 gaacccgagc ctgcctcaga cgcatatgtg ccctacgttg ccgttcctgc ccagttcgac 1320 gacaagcctc tcatctcctt cccgatcacc ctttcagatc ctgtctacca gaacaccctg 1380 gtaggcgcca tcagttcgaa cttcgccaac taccggggt gtatccaaat cactctgaca 1440 ttttgtggac ccatgatggc aagagggaaa ttcctgctct cgtattctcc cccaaatgga 1500 gcacaaccac agaccctttc tgaagctatg cagtgcacat actctatttg ggatataggc 1560 ttgaactcta gttggacctt tgtcatcccc tacatctcgc ccagtgatta ccgtgaaact 1620 cgggctatta ccaactcagt ttatctgct gatggttggt ttagcttgca caagctgacc 1680 aaaattactc taccacctga ctgcccacag agtccctgta ttctcttttt cgcctctgct 1740 ggtgaggatt acaccctccg tctccctgtt gattgtaatc cttcctacgt gttccactcc 1800 accgacaacg ccgagactgg ggttattgag gcaggtaaca ctgacaccga tttctctggt 1860 gaactggcgg ctcctggctc taaccatact aatgtcaaat tcctgtttga ccgatctcga 1920 ctactgaatg taattaaggt actggagaag gacgccgtct tcccccgtcc tttccccaca 1980 gcaacaggtg cacagcagga cgatggttac ttttgtcttc taacaccccg cccaacagtc 2040 gcttcccgac ccgccactcg tttcggcctg tacgtcaacc cgtctgacag tggcgttctc 2100 gctaacactt cactggattt caatttttac agtttggcct gtttcactta ctttagatca 2160 gaccttgaag tcacggtggt ctcactggag ccagatttgg aattcgccgt ggggtggttc 2220 ccctctggca gtgagtacca ggcttctagc tttgtctacg accaactgca tgtaccctac 2280 cactttactg ggcgcactcc ccgcgctttc accagcaagg gtggaaaggt atctttcgtg 2340 ctcccttgga actctgtctc ttccgtgctt cccgtgcgct gggggggcgc ttccaagctt 2400 tcttctgcca cgcggggtct gccggctcat gctgactggg ggaccattta cgcctttatc 2460 ccccgtccta acgagaagaa aagcaccgct gtaaagcacg tggcggtgta cgttcggtac 2520 aagaacgcgc gtgcttggtg ccccagcatg cttccctttc gcagctacaa gcaaaagatg 2580 ctgatgcaat caggcgacat cgagaccaac cctggccctg gttcaggtaa gccccctgga 2640 gcgctctctc ttatggaaat gcaacagccc aacgtggaca tgggctttga ggctgcggtc 2700 gctaagaaag tggtcgtccc cattaccttc atggttccca acagaccttc tggacttaca 2760 cagtccgctc ttcttgtggc cggccggacc ttcctaatca atgagcatac atggtccaac 2820 ccctcctgga ccagcttcac aatccgtggt gaggtgcaca ctcgtgatga gcctttccaa 2880 acggttcatt ttactcacca tggtcttccc acagatctga tgatggtacg tctcggaccg 2940 ggcaactctt tccctaacaa tctagacaag tttggacttg accagatgcc ggcacgtaac 3000 tcccgtgtgg ttggcgtttc ggctagttac ggtaacttct tcttctctgg gaacttcctc 3060 gggtttgttg actccatcac ctctgaccaa ggaacctatg cgagactttt caggtacagg 3120 gtgacgactt acaagggatg gtgcggttcg gccctggtct gtgaggccgg tggtgtccga 3180 cgcatcattg gcatgcattc tgctggtgcc gctggtatcg gcgccgggac ttacatctca 3240 aaattaggac tgatcaaagc ccttaaacac ctcggtgagc ctctggctac aatgcaagga 3300 ctgatgactg agctagagcc tggagtcacc gtacatgtac cccgaaaatc taaattgaga 3360 aagacgaccg cacacgcggt gtacaaaccg gagtttgaac ctgctgtgtt gtcaaaattt 3420 gatcccagac tgaacaagga tgttgaccta gatgaggtaa tttggtctaa acacaccgcc 3480 aacgtccctt atcaacctcc tttgttctac acatacatgt cagagtacgc tcatcgggtt 3540 ttctcctttt tgggaaaaga caatgacatt ctgaccgtca aagaagcaat cctgggcatc 3600 cctggactag accctatgga tccccacaca gctccgggtt tgccctacgc cattagcggc 3660 cttcgacgta ctgatctcgt cgattttgcg aacggcacgg tagacccggc actggccatg 3720 cagatccaga aattcttaga cggtgactac tctgatcatg tcttccaaac ttttctgaaa 3780 gatgaaatca gaccctcaga gaaggtccgg gcgggaaaaa cccgcattgt cgatgtgccc 3840 tccctggcgc actgcattgt gggcagaatg ctgcttgggc gctttgccgc caagtttcaa 3900 tcccatcctg gctttctcct tggctccgct atcgggtctg accctgatgt cttctggacc 3960 gtcatagggg ctcagctcga gggaagaaag aacacgtatg acgtggacta cagtgcctttt 4020 gactcttcac acggcactgg ctccttcgag gctctcatct ctcacttttt caccgtggac 4080 aatggttca gccctgcgct gggaccgtat ctcagatccc tggctgtctc ggtgcacgct 4140 tacggcgagc gtcgcatcaa gattaccgga ggcctcccct ctggttgtgc cgcgaccagc 4200 ctgctgaaca cagtgctcaa caatgtgatc atcaggactg ctctggcatt gacctacaag 4260 gaatttgaat atgacatggt tgatatcatc gcctacggtg acgaccttct ggttggtacg 4320 gattatgatc tggacttcaa tgaggtggcg cggcgcgctg caaactggg gtataagatg 4380 actcctgcca acaagggttc tgtcttccct ccgacttcct ctctctccga tgctgttttt 4440 ctaaaacgca aattcgtcca aaacaatgac ggcttatata aaccagttat ggatttaaag 4500 aatttggaag ccatgctctc ctacttcaaa ccaggaacac tactcgagaa gctgcaatct 4560 gttcttatgt tggctcaaca ttctggaaaa gaagaatacg atagattgat gcaccccttc 4620 gctgactatg gtgccgtacc gagtcacgag tacctgcagg caagatggag ggccttgttc 4680 gactgagcgg ccgc 4694 <210> 25 <211> 1557 <212> PRT <213> Seneca virus <400> 25 Met Gly Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly 1 5 10 15 Asn Asn Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn 20 25 30 Ser Val Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly 35 40 45 Asn Ser Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile 50 55 60 Leu Asn Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu 65 70 75 80 Asn Ser Ala Asp Arg Val Ile Thr Gln Thr Ala Gly Asn Thr Ala Ile 85 90 95 Asn Thr Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro 100 105 110 Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr 115 120 125 Phe Thr Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr 130 135 140 Lys Ala Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala 145 150 155 160 Phe Leu Ser Lys Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr 165 170 175 Leu His Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln 180 185 190 Cys Asn Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val 195 200 205 Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu 210 215 220 Gln Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg 225 230 235 240 Thr Gly Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro 245 250 255 Pro Asn Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr 260 265 270 Val Phe Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp 275 280 285 Ile Ser Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr 290 295 300 Gln Asn Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr 305 310 315 320 Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro 325 330 335 Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr 340 345 350 Asp Glu Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu 355 360 365 Met Phe Leu Ser Thr Ile Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn 370 375 380 Val Arg Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu 385 390 395 400 Leu Gln Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Gly Arg Val Ser 405 410 415 Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val Pro Tyr Val Ala Val Pro 420 425 430 Ala Gln Phe Asp Asp Lys Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser 435 440 445 Asp Pro Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe 450 455 460 Ala Asn Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro 465 470 475 480 Met Met Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly 485 490 495 Ala Gln Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile 500 505 510 Trp Asp Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Ile Pro Tyr Ile 515 520 525 Ser Pro Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr 530 535 540 Ser Ala Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu 545 550 555 560 Pro Pro Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala 565 570 575 Gly Glu Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr 580 585 590 Val Phe His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly 595 600 605 Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn 610 615 620 His Thr Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val 625 630 635 640 Ile Lys Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr 645 650 655 Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro 660 665 670 Arg Pro Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Val 675 680 685 Asn Pro Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn 690 695 700 Phe Tyr Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val 705 710 715 720 Thr Val Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe 725 730 735 Pro Ser Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu 740 745 750 His Val Pro Tyr His Phe Thr Gly Arg Thr Pro Arg Ala Phe Thr Ser 755 760 765 Lys Gly Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser 770 775 780 Val Leu Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr 785 790 795 800 Arg Gly Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Ile 805 810 815 Pro Arg Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val 820 825 830 Tyr Val Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro 835 840 845 Phe Arg Ser Tyr Lys Gln Lys Met Leu Met Gln Ser Gly Asp Ile Glu 850 855 860 Thr Asn Pro Gly Pro Gly Ser Gly Lys Pro Pro Gly Ala Leu Ser Leu 865 870 875 880 Met Glu Met Gln Gln Pro Asn Val Asp Met Gly Phe Glu Ala Ala Val 885 890 895 Ala Light Light Val Val Val Pro Ile Thr Phe Met Val Pro Asn Arg Pro 900 905 910 Ser Gly Leu Thr Gln Ser Ala Leu Leu Val Ala Gly Arg Thr Phe Leu 915 920 925 Ile Asn Glu His Thr Trp Ser Asn Pro Ser Trp Thr Ser Phe Thr Ile 930 935 940 Arg Gly Glu Val His Thr Arg Asp Glu Pro Phe Gln Thr Val His Phe 945 950 955 960 Thr His His Gly Leu Pro Thr Asp Leu Met Met Val Arg Leu Gly Pro 965 970 975 Gly Asn Ser Phe Pro Asn Asn Leu Asp Lys Phe Gly Leu Asp Gln Met 980 985 990 Pro Ala Arg Asn Ser Arg Val Val Gly Val Ser Ala Ser Tyr Gly Asn 995 1000 1005 Phe Phe Phe Ser Gly Asn Phe Leu Gly Phe Val Asp Ser Ile Thr 1010 1015 1020 Ser Asp Gln Gly Thr Tyr Ala Arg Leu Phe Arg Tyr Arg Val Thr 1025 1030 1035 Thr Tyr Lys Gly Trp Cys Gly Ser Ala Leu Val Cys Glu Ala Gly 1040 1045 1050 Gly Val Arg Arg Ile Ile Gly Met His Ser Ala Gly Ala Ala Gly 1055 1060 1065 Ile Gly Ala Gly Thr Tyr Ile Ser Lys Leu Gly Leu Ile Lys Ala 1070 1075 1080 Leu Lys His Leu Gly Glu Pro Leu Ala Thr Met Gln Gly Leu Met 1085 1090 1095 Thr Glu Leu Glu Pro Gly Val Thr Val His Val Pro Arg Lys Ser 1100 1105 1110 Lys Leu Arg Lys Thr Thr Ala His Ala Val Tyr Lys Pro Glu Phe 1115 1120 1125 Glu Pro Ala Val Leu Ser Lys Phe Asp Pro Arg Leu Asn Lys Asp 1130 1135 1140 Val Asp Leu Asp Glu Val Ile Trp Ser Lys His Thr Ala Asn Val 1145 1150 1155 Pro Tyr Gln Pro Pro Leu Phe Tyr Thr Tyr Met Ser Glu Tyr Ala 1160 1165 1170 His Arg Val Phe Ser Phe Leu Gly Lys Asp Asn Asp Ile Leu Thr 1175 1180 1185 Val Lys Glu Ala Ile Leu Gly Ile Pro Gly Leu Asp Pro Met Asp 1190 1195 1200 Pro His Thr Ala Pro Gly Leu Pro Tyr Ala Ile Ser Gly Leu Arg 1205 1210 1215 Arg Thr Asp Leu Val Asp Phe Ala Asn Gly Thr Val Asp Pro Ala 1220 1225 1230 Leu Ala Met Gln Ile Gln Lys Phe Leu Asp Gly Asp Tyr Ser Asp 1235 1240 1245 His Val Phe Gln Thr Phe Leu Lys Asp Glu Ile Arg Pro Ser Glu 1250 1255 1260 Lys Val Arg Ala Gly Lys Thr Arg Ile Val Asp Val Pro Ser Leu 1265 1270 1275 Ala His Cys Ile Val Gly Arg Met Leu Leu Gly Arg Phe Ala Ala 1280 1285 1290 Lys Phe Gln Ser His Pro Gly Phe Leu Leu Gly Ser Ala Ile Gly 1295 1300 1305 Ser Asp Pro Asp Val Phe Trp Thr Val Ile Gly Ala Gln Leu Glu 1310 1315 1320 Gly Arg Lys Asn Thr Tyr Asp Val Asp Tyr Ser Ala Phe Asp Ser 1325 1330 1335 Ser His Gly Thr Gly Ser Phe Glu Ala Leu Ile Ser His Phe Phe 1340 1345 1350 Thr Val Asp Asn Gly Phe Ser Pro Ala Leu Gly Pro Tyr Leu Arg 1355 1360 1365 Ser Leu Ala Val Ser Val His Ala Tyr Gly Glu Arg Arg Ile Lys 1370 1375 1380 Ile Thr Gly Gly Leu Pro Ser Gly Cys Ala Ala Thr Ser Leu Leu 1385 1390 1395 Asn Thr Val Leu Asn Asn Val Ile Ile Arg Thr Ala Leu Ala Leu 1400 1405 1410 Thr Tyr Lys Glu Phe Glu Tyr Asp Met Val Asp Ile Ile Ala Tyr 1415 1420 1425 Gly Asp Asp Leu Leu Val Gly Thr Asp Tyr Asp Leu Asp Phe Asn 1430 1435 1440 Glu Val Ala Arg Arg Ala Ala Lys Leu Gly Tyr Lys Met Thr Pro 1445 1450 1455 Ala Asn Lys Gly Ser Val Phe Pro Pro Thr Ser Ser Leu Ser Asp 1460 1465 1470 Ala Val Phe Leu Lys Arg Lys Phe Val Gln Asn Asn Asp Gly Leu 1475 1480 1485 Tyr Lys Pro Val Met Asp Leu Lys Asn Leu Glu Ala Met Leu Ser 1490 1495 1500 Tyr Phe Lys Pro Gly Thr Leu Leu Glu Lys Leu Gln Ser Val Ser 1505 1510 1515 Met Leu Ala Gln His Ser Gly Lys Glu Glu Tyr Asp Arg Leu Met 1520 1525 1530 His Pro Phe Ala Asp Tyr Gly Ala Val Pro Ser His Glu Tyr Leu 1535 1540 1545 Gln Ala Arg Trp Arg Ala Leu Phe Asp 1550 1555 <210> 26 <211> 2618 <212> DNA <213> Seneca virus <400> 26 ggatccgcca ccatgggtaa tgttcagaca acctcaaaga atgattttga ttcccgcggc 60 aataatggta acatgacctt caattactac gcaaacactt accagaattc agtagacttc 120 tcgacctcct cgtcggcgtc aggcgccgga cccgggaact cccggggcgg actagcgggt 180 ctcctcacaa atttcagtgg aatcttgaac cctcttggct acctcaaaga tcacaatacc 240 gaagaaatgg aaaactctgc tgatcgagtc ataacgcaaa cggcgggcaa cactgccata 300 aacacgcaat catcactggg tgtgttgtgt gcctacgttg aagacccgac caaatctgac 360 cctccgtcca gcagcacaga tcaacccacc accactttta ctgccatcga caggtggtac 420 actggacgcc tcaattcttg gacaaaagct gtaaaaaacct tctcttttca ggccgtcccg 480 ctccctggag ccttcctgtc taaacaggga ggcctcaacg gaggggcctt cacggctacc 540 ctacatagac atttcttaat gaagtgcggg tggcaggtgc aggtccaatg caatttgacg 600 caattccacc aaggtgctct tcttgttgcc atggtccccg aaaccaccct tgatgtcaaa 660 cctgacggca aggcaaagag cttacaggag ctgaatgaag agcagtgggt ggaaatgtct 720 gacgattacc ggaccgggaa aaacatgcct tttcagtctc ttggcacata ctatcggccc 780 cctaactgga cttggggccc caatttcatc aacccctatc aagtaacagt tttcccacac 840 caaattctga acgcgagaac ctctacctcg gtagacataa gtgtcccata catcggggag 900 actcctacac aatcctcaga gacacagaac tcctggaccc tccttgttat ggtgcttgtc 960 cccctggact acaaggggg agccacaact gacccagaaa ttacattttc tgtaaggcct 1020 acaagtccct acttcaatgg gcttcgtaac cgtttcacga ccgggacgga cgaggaacag 1080 gggcccattc ccacagcacc cagagaaaat tcgcttatgt ttctctcaac catccctgac 1140 gacactgtcc ctgcttacgg gaatgtgcgt acccctcccg tcaattacct ccccggtgaa 1200 ataaccgacc tcttacaact ggcccgtata cccactctca tggcgtttgg gcgggtgtct 1260 gaacccgagc ctgcctcaga cgcatatgtg ccctacgttg ccgttcctgc ccagttcgac 1320 gacaagcctc tcatctcctt cccgatcacc ctttcagatc ctgtctacca gaacaccctg 1380 gtaggcgcca tcagttcgaa cttcgccaac taccggggt gtatccaaat cactctgaca 1440 ttttgtggac ccatgatggc aagagggaaa ttcctgctct cgtattctcc cccaaatgga 1500 gcacaaccac agaccctttc tgaagctatg cagtgcacat actctatttg ggatataggc 1560 ttgaactcta gttggacctt tgtcatcccc tacatctcgc ccagtgatta ccgtgaaact 1620 cgggctatta ccaactcagt ttatctgct gatggttggt ttagcttgca caagctgacc 1680 aaaattactc taccacctga ctgcccacag agtccctgta ttctcttttt cgcctctgct 1740 ggtgaggatt acaccctccg tctccctgtt gattgtaatc cttcctacgt gttccactcc 1800 accgacaacg ccgagactgg ggtattgag gcaggtaaca ctgacaccga tttctctggt 1860 gaactggcgg ctcctggctc taaccatact aatgtcaaat tcctgtttga ccgatctcga 1920 ctactgaatg taattaaggt actggagaag gacgccgtct tcccccgtcc tttccccaca 1980 gcaacaggtg cacagcagga cgatggttac ttttgtcttc taacaccccg cccaacagtc 2040 gcttcccgac ccgccactcg tttcggcctg tacgtcaacc cgtctgacag tggcgttctc 2100 gctaacactt cactggattt caatttttac agtttggcct gtttcactta ctttagatca 2160 gaccttgaag tcacggtggt ctcactggag ccagatttgg aattcgccgt ggggtggttc 2220 ccctctggca gtgagtacca ggcttctagc tttgtctacg accaactgca tgtaccctac 2280 cactttactg ggcgcactcc ccgcgctttc accagcaagg gtggaaaggt atctttcgtg 2340 ctcccttgga actctgtctc ttccgtgctt cccgtgcgct gggggggcgc ttccaagctt 2400 tcttctgcca cgcggtgtct gccggctcat gctgactggg ggaccattta cgcctttatc 2460 ccccgtccta acgagaagaa aagcaccgct gtaaagcacg tggcggtgta cgttcggtac 2520 aagaacgcgc gtgcttggtg ccccagcatg cttccctttc gcagctacaa gcaaaagatg 2580 ctgatgcaac accaccacca ccaccactga gcggccgc 2618 <210> 27 <211> 855 <212> PRT <213> Seneca virus <400> 27 Met Gly Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly 1 5 10 15 Asn Asn Gly Asn Met Thr Phe Asn Tyr Tyr Ala Asn Thr Tyr Gln Asn 20 25 30 Ser Val Asp Phe Ser Thr Ser Ser Ser Ala Ser Gly Ala Gly Pro Gly 35 40 45 Asn Ser Arg Gly Gly Leu Ala Gly Leu Leu Thr Asn Phe Ser Gly Ile 50 55 60 Leu Asn Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu 65 70 75 80 Asn Ser Ala Asp Arg Val Ile Thr Gln Thr Ala Gly Asn Thr Ala Ile 85 90 95 Asn Thr Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro 100 105 110 Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr 115 120 125 Phe Thr Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr 130 135 140 Lys Ala Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala 145 150 155 160 Phe Leu Ser Lys Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr 165 170 175 Leu His Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln 180 185 190 Cys Asn Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val 195 200 205 Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu 210 215 220 Gln Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg 225 230 235 240 Thr Gly Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro 245 250 255 Pro Asn Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr 260 265 270 Val Phe Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp 275 280 285 Ile Ser Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr 290 295 300 Gln Asn Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr 305 310 315 320 Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro 325 330 335 Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr 340 345 350 Asp Glu Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu 355 360 365 Met Phe Leu Ser Thr Ile Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn 370 375 380 Val Arg Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu 385 390 395 400 Leu Gln Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Gly Arg Val Ser 405 410 415 Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val Pro Tyr Val Ala Val Pro 420 425 430 Ala Gln Phe Asp Asp Lys Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser 435 440 445 Asp Pro Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe 450 455 460 Ala Asn Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro 465 470 475 480 Met Met Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly 485 490 495 Ala Gln Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile 500 505 510 Trp Asp Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Ile Pro Tyr Ile 515 520 525 Ser Pro Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr 530 535 540 Ser Ala Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu 545 550 555 560 Pro Pro Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala 565 570 575 Gly Glu Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr 580 585 590 Val Phe His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly 595 600 605 Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn 610 615 620 His Thr Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val 625 630 635 640 Ile Lys Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr 645 650 655 Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro 660 665 670 Arg Pro Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Val 675 680 685 Asn Pro Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn 690 695 700 Phe Tyr Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val 705 710 715 720 Thr Val Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe 725 730 735 Pro Ser Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu 740 745 750 His Val Pro Tyr His Phe Thr Gly Arg Thr Pro Arg Ala Phe Thr Ser 755 760 765 Lys Gly Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser 770 775 780 Val Leu Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr 785 790 795 800 Arg Cys Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Ile 805 810 815 Pro Arg Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val 820 825 830 Tyr Val Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro 835 840 845 Phe Arg Ser Tyr Lys Gln Lys 850 855 <210> 28 <211> 2618 <212> DNA <213> Seneca virus <400> 28 ggatccgcca ccatgggcaa cgttcaaact acctccaaga atgattttga ctcccgcggc 60 aacaacggca acatgacttt taactactac gccaatacgt atcaaaactc tgttgatttc 120 tcgacatcga gttccgcttc cggcgcggga cctggaaatt cccgcggtgg actggccggt 180 ctgctcacaa acttttccgg tatcctgaac cccctcggtt atctgaaaga tcacaatacg 240 gaagagatgg agaactctgc cgatcgtgtg attacgcaga cagctggtaa cacggctatc 300 aacactcaat cgtctctggg agtactctgt gcttacgtcg aggacccaac aaaatccgac 360 cctccatctt cctcgaccga tcaacctacg actaccttca cggccataga caggtggtat 420 acaggtcgcc tgaattcgtg gacaaaggct gttaaaacct ttagcttcca ggctgtgcca 480 ttgcccggtg ctttcctttc ccgccagggc ggcctcaacg gtggagcttt tactgctaca 540 ctgcacagac actttctgat gaaatgcggt tggcaggtgc aggtacagtg caatttgact 600 caattccacc aaggtgcact tctcgttgct atggttcccg agactacgct tgacgttaag 660 cctgacggca aggccaagtc tctccaagag ctgaatgagg aacagtgggt tgagatgagc 720 gatgactata gaaccggtaa aaacatgcca ttccagtccc tgggcacata ctacagaccc 780 ccaaattgga cttggggccc gaactttatc aatccgtatc aggtcaccgt gtttccacac 840 caaatcctga atgccaggac tagtaccagc gtcgatatta gcgtgcccta tatcggcgaa 900 acgccaaccc aatcctccga aactcagaac agctggacgc ttctggtaat ggtgttggta 960 cccttggatt ataaggaggg agccactacc gaccctgaaa tcactttttc ggtaaggccc 1020 actagccctt acttcaacgg actgcgcaac cgtttcacca ccggcactga tgaggagcaa 1080 ggccctatcc ctactgcccc cagagaaaat agcctgatgt ttctgagcac aattcctgac 1140 gacaccgtac ctgcctatgg taacgttcgt acgcctcccg taaactacct gcctggtgaa 1200 attacggacc tcttgcagct ggcccgcatc cctacactga tggccttcgg tcgtgtcagt 1260 gagcccgaac cagccagcga cgcttacgtt ccatatgttg cggtacctgc tcagttcgac 1320 gacaagccgt tgataagttt cccaatcact ttgagcgacc ctgtatacca gaacactttg 1380 gttggagcta tctcctcgaa ctttgcgaac taccgtggtt gtattcaaat cacccttaca 1440 ttctgcggcc cgatgatggc tcgtggcaag ttcctgttga gctactcccc ccccaacgga 1500 gcacaaccac agacactgtc cgaggccatg cagtgcacat actccatctg ggatatcggt 1560 ctcaacagct catggacctt tgfather tacatctcgc catccgatta tagagagacc cgcgccatca ccaacagtgt gtactcagcc gatggatggt tcagtctcca tagcttaca aagatcactc tgccccccga ctgcccacaa tcaccctgca tattgttctt tgcctcagct 1740 ggtgaagatt acacgcttcg tttgcccgtc gactgtaacc cgtcttacgt gttccactcc acggacaatg ctgagaccgg tgtaattgag gctggtaaca ccgacacgga tttctctggt gagctggcgg ctcccggttc taaccatacc aacgtgaagt tccttttcga ccgttcacgt cttttgaacg taatcaaggt cttggaaaag gacgctgtgt tccctcgccc attccccaca gcaacgggag cacaacaaga cgacggctat ttctgcctgc tgacaccacg tcctactgtg gctagccgtc cggccacacg ctttggtctc tacgttaacc ctagcgactc tggtgtactg gctaacacct ccctggactt caatttctac tctctggcct gcttcacgta cttcaggagc gacctggagg tcactgtggt gtctctgga ccagatctgg aatttgcggt aggatggttc 2220 ccttcagga gcgagtacca ggcatcgtcc tttgtgtacg accaacttca tgttccctac catttcaccg gtagaacccc acgtgccttc accagtaagg gaggcaaagt ttcttttgta 2340 ctgccctgga attctgtcag ttccgttctc ccggtccgct ggggcggagc gtcaaagctc 2400 tcatccgcga cccgcggatt gcccgcacac gcggattggg gaactatcta cgctttcatt 2460 ccacgcccta atgaaaagaa gtctacagca gtgaagcacg tggccgtcta tgtgcgttac 2520 aagaatgcaa gggcgtggtg tccgtctatg ttgccattcc gtagctataa acagaagatg 2580 cttatgcaac accaccacca ccaccactga gcggccgc 2618 <210> 29 <211> 865 <212> PRT <213> Seneca virus <400> 29 Met Gly Asn Val Gln Thr Thr Ser Lys Asn Asp Phe Asp Ser Arg Gly<0​​​​​​​​​​​​​​Leu Asn Pro Leu Gly Tyr Leu Lys Asp His Asn Thr Glu Glu Met Glu 65 70 75 80 Asn Ser Ala Asp Arg Val Ile Thr Gln Thr Ala Gly Asn Thr Ala Ile 85 90 95 Asn Thr Gln Ser Ser Leu Gly Val Leu Cys Ala Tyr Val Glu Asp Pro 100 105 110 Thr Lys Ser Asp Pro Pro Ser Ser Ser Thr Asp Gln Pro Thr Thr Thr 115 120 125 Phe Thr Ala Ile Asp Arg Trp Tyr Thr Gly Arg Leu Asn Ser Trp Thr 130 135 140 Lys Ala Val Lys Thr Phe Ser Phe Gln Ala Val Pro Leu Pro Gly Ala 145 150 155 160 Phe Leu Ser Arg Gln Gly Gly Leu Asn Gly Gly Ala Phe Thr Ala Thr 165 170 175 Leu His Arg His Phe Leu Met Lys Cys Gly Trp Gln Val Gln Val Gln 180 185 190 Cys Asn Leu Thr Gln Phe His Gln Gly Ala Leu Leu Val Ala Met Val 195 200 205 Pro Glu Thr Thr Leu Asp Val Lys Pro Asp Gly Lys Ala Lys Ser Leu 210 215 220 Gln Glu Leu Asn Glu Glu Gln Trp Val Glu Met Ser Asp Asp Tyr Arg 225 230 235 240 Thr Gly Lys Asn Met Pro Phe Gln Ser Leu Gly Thr Tyr Tyr Arg Pro 245 250 255 Pro Asn Trp Thr Trp Gly Pro Asn Phe Ile Asn Pro Tyr Gln Val Thr 260 265 270 Val Phe Pro His Gln Ile Leu Asn Ala Arg Thr Ser Thr Ser Val Asp 275 280 285 Ile Ser Val Pro Tyr Ile Gly Glu Thr Pro Thr Gln Ser Ser Glu Thr 290 295 300 Gln Asn Ser Trp Thr Leu Leu Val Met Val Leu Val Pro Leu Asp Tyr 305 310 315 320 Lys Glu Gly Ala Thr Thr Asp Pro Glu Ile Thr Phe Ser Val Arg Pro 325 330 335 Thr Ser Pro Tyr Phe Asn Gly Leu Arg Asn Arg Phe Thr Thr Gly Thr 340 345 350 Asp Glu Glu Gln Gly Pro Ile Pro Thr Ala Pro Arg Glu Asn Ser Leu 355 360 365 Met Phe Leu Ser Thr Ile Pro Asp Asp Thr Val Pro Ala Tyr Gly Asn 370 375 380 Val Arg Thr Pro Pro Val Asn Tyr Leu Pro Gly Glu Ile Thr Asp Leu 385 390 395 400 Leu Gln Leu Ala Arg Ile Pro Thr Leu Met Ala Phe Gly Arg Val Ser 405 410 415 Glu Pro Glu Pro Ala Ser Asp Ala Tyr Val Pro Tyr Val Ala Val Pro 420 425 430 Ala Gln Phe Asp Asp Lys Pro Leu Ile Ser Phe Pro Ile Thr Leu Ser 435 440 445 Asp Pro Val Tyr Gln Asn Thr Leu Val Gly Ala Ile Ser Ser Asn Phe 450 455 460 Ala Asn Tyr Arg Gly Cys Ile Gln Ile Thr Leu Thr Phe Cys Gly Pro 465 470 475 480 Met Met Ala Arg Gly Lys Phe Leu Leu Ser Tyr Ser Pro Pro Asn Gly 485 490 495 Ala Gln Pro Gln Thr Leu Ser Glu Ala Met Gln Cys Thr Tyr Ser Ile 500 505 510 Trp Asp Ile Gly Leu Asn Ser Ser Trp Thr Phe Val Ile Pro Tyr Ile 515 520 525 Ser Pro Ser Asp Tyr Arg Glu Thr Arg Ala Ile Thr Asn Ser Val Tyr 530 535 540 Ser Ala Asp Gly Trp Phe Ser Leu His Lys Leu Thr Lys Ile Thr Leu 545 550 555 560 Pro Pro Asp Cys Pro Gln Ser Pro Cys Ile Leu Phe Phe Ala Ser Ala 565 570 575 Gly Glu Asp Tyr Thr Leu Arg Leu Pro Val Asp Cys Asn Pro Ser Tyr 580 585 590 Val Phe His Ser Thr Asp Asn Ala Glu Thr Gly Val Ile Glu Ala Gly 595 600 605 Asn Thr Asp Thr Asp Phe Ser Gly Glu Leu Ala Ala Pro Gly Ser Asn 610 615 620 His Thr Asn Val Lys Phe Leu Phe Asp Arg Ser Arg Leu Leu Asn Val 625 630 635 640 Ile Lys Val Leu Glu Lys Asp Ala Val Phe Pro Arg Pro Phe Pro Thr 645 650 655 Ala Thr Gly Ala Gln Gln Asp Asp Gly Tyr Phe Cys Leu Leu Thr Pro 660 665 670 Arg Pro Thr Val Ala Ser Arg Pro Ala Thr Arg Phe Gly Leu Tyr Val 675 680 685 Asn Pro Ser Asp Ser Gly Val Leu Ala Asn Thr Ser Leu Asp Phe Asn 690 695 700 Phe Tyr Ser Leu Ala Cys Phe Thr Tyr Phe Arg Ser Asp Leu Glu Val 705 710 715 720 Thr Val Val Ser Leu Glu Pro Asp Leu Glu Phe Ala Val Gly Trp Phe 725 730 735 Pro Ser Gly Ser Glu Tyr Gln Ala Ser Ser Phe Val Tyr Asp Gln Leu 740 745 750 His Val Pro Tyr His Phe Thr Gly Arg Thr Pro Arg Ala Phe Thr Ser 755 760 765 Lys Gly Gly Lys Val Ser Phe Val Leu Pro Trp Asn Ser Val Ser Ser 770 775 780 Val Leu Pro Val Arg Trp Gly Gly Ala Ser Lys Leu Ser Ser Ala Thr 785 790 795 800 Arg Gly Leu Pro Ala His Ala Asp Trp Gly Thr Ile Tyr Ala Phe Ile 805 810 815 Pro Arg Pro Asn Glu Lys Lys Ser Thr Ala Val Lys His Val Ala Val 820 825 830 Tyr Val Arg Tyr Lys Asn Ala Arg Ala Trp Cys Pro Ser Met Leu Pro 835 840 845 Phe Arg Ser Tyr Lys Gln Lys Met Leu Met Gln His His His His His 850 855 860 His 865 <210> 30 <211> 651 <212> DNA <213> Seneca virus <400> 30 actagtatgc agcccaacgt ggacatgggc tttgaggctg cggtcgctaa gaaagtggtc 60 gtccccatta ccttcatggt tcccaacaga ccttctggac ttacacagtc cgctcttctt 120 gtggccggcc ggaccttcat aatcaatgag catacatggt ccaacccctc ctggaccagc 180 ttcacaatcc gtggtgaggt gcacactcgt gatgagcctt tccaaacggt tcattttact 240 caccatggtc ttcccacaga tctgatgatg gtacgtctcg gaccgggcaa ctctttccct 300 aacaatctag acaagtttgg acttgaccag atgccggcac gtaactcccg tgtggttggc 360 gtttcggcta gttacggtaa cttcttcttc tctgggaact tcctcgggtt tgttgactcc 420[[ID=-1]] atcacctctg accaaggaac ctatgcgaga cttttcaggt acagggtgac gacttacaag 480 ggatggtgcg gttcggccct ggtctgtgag gccggtggtg tccgacgcat cattggcatg 540 cattctgctg gtgccgctgg tatcggcgcc gggacttaca tctcaaaatt aggactgatc 600 aaagccctta aacacctcgg tgagcctctg gctacaatgc aatgagagct c 651 <210> 31 <211> 212 <212> PRT <213> Seneca virus <400> 31 Met Gln Pro Asn Val Asp Met Gly Phe Glu Ala Ala Val Ala Lys Lys {1} {5} {10} {15} Val Val Val Pro Ile Thr Phe Met Val Pro Asn Arg Pro Ser Gly Leu {20} {25} {30}<00> Thr Gln Ser Ala Leu Leu Val Ala Gly Arg Thr Phe Ile Ile Asn Glu {35} {4} {45} His Thr Trp Ser Asn Pro Ser Trp Thr Ser Phe Thr Ile Arg Gly Glu {50} {55} {60} Val His Thr Arg Asp Glu Pro Phe Gln Thr Val His Phe Thr His His {65} {70} {75} {80} Gly Leu Pro Thr Asp Leu Met Met Val Arg Leu Gly Pro Gly Asn Ser {85} {90} {95} Phe Pro Asn Asn Leu Asp Lys Phe Gly Leu Asp Gln Met Pro Ala Arg [[ID=--40]]{100} {105} {110} Note: In the translation, the numbers within the curly braces are added for better alignment with the original text's format indicating positions. The original text's format seems a bit irregular in terms of how the positions are presented, and this is an attempt to maintain a similar visual structure in the translation. Also, the "aaagccctta aacacctcgg tgagcctctg gctacaatgc aatgagagct c 651" in line might have some incorrect spacing or encoding in the original, but it's translated as is according to the rules.Asn Ser Arg Val Val Gly Val Ser Ala Ser Tyr Gly Asn Phe Phe Phe 115 120 125 Ser Gly Asn Phe Leu Gly Phe Val Asp Ser Ile Thr Ser Asp Gln Gly 130 135 140 Thr Tyr Ala Arg Leu Phe Arg Tyr Arg Val Thr Thr Tyr Lys Gly Trp 145 150 155 160 Cys Gly Ser Ala Leu Val Cys Glu Ala Gly Gly Val Arg Arg Ile Ile 165 170 175 Gly Met His Ser Ala Gly Ala Ala Gly Ile Gly Ala Gly Thr Tyr Ile 180 185 190 Ser Lys Leu Gly Leu Ile Lys Ala Leu Lys His Leu Gly Glu Pro Leu 195 200 205 Ala Thr Met Gln 210 <210> 32 <211> 3489 <212> DNA <213> Seneca virus[[ID=3�]] <400> 32 ggatccgcca ccatgggtaa tgttcagaca acctcaaaga atgattttga ttcccgcggc 60<t000४204>aataatggta acatgacctt caattactac gcaaacactt accagaattc agtagacttc 120 tcgacctcct cgtcggcgtc aggcgccgga cccgggaact cccggggcgg actagcgggt 180 ctcctcacaa atttcagtgg aatcttgaac cctcttggct acctcaaaga tcacaatacc 240 gaagaaatgg aaaactctgc tgatcgagtc ataacgcaaa cggcgggcaa cactgccata 300 aacacgcaat catcactggg tgtgttgtgt gcctacgttg aagacccgac caaatctgac 360 cctccgtcca gcagcacaga tcaacccacc accactttta ctgccatcga caggtggtac 420 actggacgcc tcaattcttg gacaaaagct gtaaaaaacct tctcttttca ggccgtcccg 480 ctccctggag ccttcctgtc taaacaggga ggcctcaacg gaggggcctt cacggctacc 540 ctacatagac atttcttaat gaagtgcggg tggcaggtgc aggtccaatg caatttgacg 600 caattccacc aaggtgctct tcttgttgcc atggtccccg aaaccaccct tgatgtcaaa 660 cctgacggca aggcaaagag cttacaggag ctgaatgaag agcagtgggt ggaaatgtct 720 gacgattacc ggaccgggaa aaacatgcct tttcagtctc ttggcacata ctatcggccc 780 cctaactgga cttggggccc caatttcatc aacccctatc aagtaacagt tttcccacac 840 caaattctga acgcgagaac ctctacctcg gtagacataa gtgtcccata catcggggag 900 actcctacac aatcctcaga gacacagaac tcctggaccc tccttgttat ggtgcttgtc 960 cccctggact acaaggaggg agccacaact gacccagaaa ttacattttc tgtaaggcct 1020 acaagtccct acttcaatgg gcttcgtaac cgtttcacga ccgggacgga cgaggaacag 1080 gggcccattc ccacagcacc cagagaaaat tcgcttatgt ttctctcaac catccctgac 1140 gacactgtcc ctgcttacgg gaatgtgcgt acccctcccg tcaattacct ccccggtgaa 1200 ataaccgacc tcttacaact ggcccgtata cccactctca tggcgtttgg gcgggtgtct 1260 gaacccgagc ctgcctcaga cgcatatgtg ccctacgttg ccgttcctgc ccagttcgac 1320 gacaagcctc tcatctcctt cccgatcacc ctttcagatc ctgtctacca gaacaccctg 1380 gtaggcgcca tcagttcgaa cttcgccaac taccgggggt gtatccaaat cactctgaca 1440 ttttgtggac ccatgatggc aagagggaaa ttcctgctct cgtattctcc cccaaatgga 1500 gcacaaccac agaccctttc tgaagctatg cagtgcacat actctatttg ggatataggc 1560 ttgaactcta gttggacctt tgtcatcccc tacatctcgc ccagtgatta ccgtgaaact 1620 cgggctatta ccaactcagt ttattctgct gatggttggt ttagcttgca caagctgacc 1680 aaaattactc taccacctga ctgcccacag agtccctgta ttctcttttt cgcctctgct 1740 ggtgaggatt acaccctccg tctccctgtt gattgtaatc cttcctacgt gttccactcc 1800 accgacaacg ccgagactgg ggttattgag gcaggtaaca ctgacaccga tttctctggt 1860 gaactggcgg ctcctggctc taaccatact aatgtcaaat tcctgtttga ccgatctcga 1920 ctactgaatg taattaaggt actggagaag gacgccgtct tcccccgtcc tttccccaca 1980 gcaacaggtg cacagcagga cgatggttac ttttgtcttc taacaccccg cccaacagtc 2040 gcttcccgac ccgccactcg tttcggcctg tacgtcaacc cgtctgacag tggcgttctc 2100 gctaacactt cactggattt caatttttac agtttggcct gtttcactta ctttagatca 2160 gaccttgaag tcacggtggt ctcactggag ccagatttgg aattcgccgt ggggtggttc 2220 ccctctggca gtgagtacca ggcttctagc tttgtctacg accaactgca tgtaccctac 2280 cactttactg ggcgcactcc ccgcgctttc accagcaagg gtggaaaggt atctttcgtg 2340 ctcccttgga actctgtctc ttccgtgctt cccgtgcgct gggggggcgc ttccaagctt 2400 tcttctgcca cgcggtgtct gccggctcat gctgactggg ggaccattta cgcctttatc 2460 ccccgtccta acgagaagaa aagcaccgct gtaaagcacg tggcggtgta cgttcggtac 2520 aagaacgcgc gtgcttggtg ccccagcatg cttccctttc gcagctacaa gcaaaagatg 2580 ctgatgcaac accaccacca ccaccactga gcggccgcga actgtggagc cagggcggcc 2640 ttggccaccc tggcttactg taacagaaaa aagagtaaaa ggcgacagct cgcttgccaa 2700 ttgtcctgtt acgtactctg tggtttcacg aggttgtcat caccaaaggt aacctttttt 2760 tttgtcctcg ccgacaaaac gacatcttaa taaccaagca acgttcgata aagaaaaaaa 2820 ctcgtcctta agtcgcgaac tagtatgcag cccaacgtgg acatgggctt tgaggctgcg 2880 gtcgctaaga aagtggtcgt ccccattacc ttcatggttc ccaacagacc ttctggactt 2940 acacagtccg ctcttcttgt ggccggccgg accttcataa tcaatgagca tacatggtcc 3000 aacccctcct ggaccagctt cacaatccgt ggtgaggtgc acactcgtga tgagcctttc 3060 caaacggttc attttactca ccatggtctt cccacagatc tgatgatggt acgtctcgga 3120 ccgggcaact ctttccctaa caatctagac aagtttggac ttgaccagat gccggcacgt 3180 aactcccgtg tggttggcgt ttcggctagt tacggtaact tcttcttctc tgggaacttc 3240 ctcgggtttg ttgactccat cacctctgac caaggaacct atgcgagact tttcaggtac 3300 agggtgacga cttacaaggg atggtgcggt tcggccctgg tctgtgaggc cggtggtgtc 3360 cgacgcatca ttggcatgca ttctgctggt gccgctggta tcggcgccgg gacttacatc 3420 tcaaaattag gactgatcaa agcccttaaa cacctcggtg agcctctggc tacaatgcaa 3480 tgagagctc 3489 <210> 33 <211> 3489 <212> DNA <213> Seneca virus <400> 33 ggatccgcca ccatgggcaa cgttcaaact acctccaaga atgattttga ctcccgcggc 60 aacaacggca acatgacttt taactactac gccaatacgt atcaaaactc tgttgatttc 120 tcgacatcga gttccgcttc cggcgcggga cctggaaatt cccgcggtgg actggccggt 180 ctgctcacaa acttttccgg tatcctgaac cccctcggtt atctgaaaga tcacaatacg 240 gaagagatgg agaactctgc cgatcgtgtg attacgcaga cagctggtaa cacggctatc 300 aacactcaat cgtctctggg agtactctgt gcttacgtcg aggacccaac aaaatccgac 360 cctccatctt cctcgaccga tcaacctacg actaccttca cggccataga caggtggtat 420 acaggtcgcc tgaattcgtg gacaaaggct gttaaaacct ttagcttcca ggctgtgcca 480 ttgcccggtg ctttcctttc ccgccagggc ggcctcaacg gtggagcttt tactgctaca 540 ctgcacagac actttctgat gaaatgcggt tggcaggtgc aggtacagtg caatttgact 600 caattccacc aaggtgcact tctcgttgct atggttcccg agactacgct tgacgttaag 660 cctgacggca aggccaagtc tctccaagag ctgaatgagg aacagtgggt tgagatgagc 720 gatgactata gaaccggtaa aaacatgcca ttccagtccc tgggcacata ctacagaccc 780 ccaaattgga cttggggccc gaactttatc aatccgtatc aggtcaccgt gtttccacac 840 caaatcctga atgccaggac tagtaccagc gtcgatatta gcgtgcccta tatcggcgaa 900 acgccaaccc aatcctccga aactcagaac agctggacgc ttctggtaat ggtgttggta 960 cccttggatt ataaggaggg agccactacc gaccctgaaa tcactttttc ggtaaggccc 1020 actagccctt acttcaacgg actgcgcaac cgtttcacca ccggcactga tgaggagcaa 1080 ggccctatcc ctactgcccc cagagaaaat agcctgatgt ttctgagcac aattcctgac 1140 gacaccgtac ctgcctatgg taacgttcgt acgcctcccg taaactacct gcctggtgaa 1200 attacggacc tcttgcagct ggcccgcatc cctacactga tggccttcgg tcgtgtcagt 1260 gagcccgaac cagccagcga cgcttacgtt ccatatgttg cggtacctgc tcagttcgac 1320 gacaagccgt tgataagttt cccaatcact ttgagcgacc ctgtatacca gaacactttg 1380 gttggagcta tctctcgaa ctttgcgaac taccgtggtt gtattcaaat cacccttaca 1440 ttctgcggcc cgatgatggc tcgtggcaag ttcctgttga gctactcccc ccccaacgga 1500 gcacaaccac agacactgtc cgaggccatg cagtgcacat actccatctg ggatatcggt 1560 ctcaacagct catggacctt tgtaatacca tacatctcgc catccgatta tagagagacc 1620 cgcgccatca ccaacagtgt gtactcagcc gatggatggt tcagtctcca taagcttaca 1680 aagatcactc tgccccccga ctgcccacaa tcaccctgca tattgttctt tgcctcagct 1740 ggtgaagatt acacgcttcg tttgcccgtc gactgtaacc cgtcttacgt gttccactcc 1800 acggacaatg ctgagaccgg tgtaattgag gctggtaaca ccgacacgga tttctctggt 1860 gagctggcgg ctcccggttc taaccatacc aacgtgaagt tccttttcga ccgttcacgt 1920 cttttgaacg taatcaaggt cttggaaaag gacgctgtgt tccctcgccc attccccaca 1980 gcaacgggag cacaacaaga cgacggctat ttctgcctgc tgacaccacg tcctactgtg 2040 gctagccgtc cggccacacg ctttggtctc tacgttaacc ctagcgactc tggtgtactg 2100 gctaacacct ccctggactt caatttctac tctctggcct gcttcacgta cttcaggagc 2160 gacctggagg tcactgtggt gtctctggaa ccagatctgg aatttgcggt aggatggttc 2220 ccttcaggaa gcgagtacca ggcatcgtcc tttgtgtacg accaacttca tgttccctac 2280 catttcaccg gtagaacccc acgtgccttc accagtaagg gaggcaaagt ttctttgta 2340 ctgccctgga attctgtcag ttccgttctc ccggtccgct ggggcggagc gtcaaagctc 2400 tcatccgcga cccgcggatt gcccgcacac gcggattggg gaactatcta cgctttcatt 2460 ccacgcccta atgaaaagaa gtctacagca gtgaagcacg tggccgtcta tgtgcgttac 2520 aagaatgcaa gggcgtggtg tccgtctatg ttgccattcc gtagctataa acagaagatg 2580 cttatgcaac accaccacca ccaccactga gcggccgcga actgtggagc cagggcggcc 2640 ttggccaccc tggcttactg taacagaaaa aagagtaaaa ggcgacagct cgcttgccaa 2700 ttgtcctgtt acgtactctg tggtttcacg aggttgtcat caccaaaggt aacctttttt 2760 tttgtcctcg ccgacaaaac gacatcttaa taaccaagca acgttcgata aagaaaaaaa 2820 ctcgtcctta agtcgcgaac tagtatgcag cccaacgtgg acatgggctt tgaggctgcg 2880 gtcgctaaga aagtggtcgt ccccattacc ttcatggttc ccaacagacc ttctggactt 2940 acacagtccg ctcttcttgt ggccggccgg accttcataa tcaatgagca tacatggtcc 3000 aacccctcct ggaccagctt cacaatccgt ggtgaggtgc acactcgtga tgagcctttc 3060 caaacggttc attttactca ccatggtctt cccacagatc tgatgatggt acgtctcgga 3120 ccgggcaact ctttccctaa caatctagac aagtttggac ttgaccagat gccggcacgt 3180 aactcccgtg tggttggcgt ttcggctagt tacggtaact tcttcttctc tgggaacttc 3240 ctcgggtttg ttgactccat cacctctgac caaggaacct atgcgagact tttcaggtac 3300 agggtgacga cttacaaggg atggtgcggt tcggccctgg tctgtgaggc cggtggtgtc 3360 cgacgcatca ttggcatgca ttctgctggt gccgctggta tcggcgccgg gacttacatc 3420 tcaaaattag gactgatcaa agcccttaaa cacctcggtg agcctctggc tacaatgcaa 3480 tgagagctc 3489 <210> 34 <211> 26 <212> DNA <213> Artificial sequence <220> <223> Primer <400> 34 ggatccgcca ccatgggtaa tgttca 26 <210> 35 <211> 58 <212> DNA <213> Artificial sequence <220> <223> Primer <400> 35 gcggccgctc agtggtggtg gtggtggtgt tgcatcagca tcttttgctt gtagctgc 58 <210> 36 <211> twenty two <212> DNA <213> Artificial sequence <220> <223> Primers <400> 36 ggatccgcca ccatgggcaa cg 22 <210> 37 <211> 60 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 37 gcggccgctc agtggtggtg gtggtggtgt tgcataagca tcttctgttt atagctacgg 60 <210> 38 <211> 33 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 38 actagtatgc agcccaacgt ggacatgggc ttt 33 <210> 39 <211> 35 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 39 gagctctcat tgcattgtag ccagaggctc accga 35 <210> 40 <211> 41 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 40 cttcctacgt gcctcagggg gttgacaacg ccgagactgg g 41 <210> 41 <211> 41 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 41 cccagtctcg gcgttgtcaa ccccctgagg cacgtaggaa g 41 <210> 42 <211> 35 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 42 tacaatgcaa ggactgatga ctgagctaga gcctg 35 <210> 43 <211> 31 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 43 tcagtcatca gtccttgcat tgtagccaga g 31 <210> 44 <211> 32 <212> DNA <213> Artificial sequence <220> <223> Primers <400> 44 gcggccgctc agtcgaacaa ggccctccat ct 32 <210> 45 <211> 1389 <212> DNA <213> Seneca virus <400> 45 ggactgatga ctgagctaga gcctggagtc accgtacatg taccccgaaa atctaaattg 60 agaaagacga ccgcacacgc ggtgtacaaa ccggagtttg aacctgctgt gttgtcaaaa 120 tttgatccca gactgaacaa ggatgttgac ctagatgagg taatttggtc taaacacacc 180 gccaacgtcc cttatcaacc tcctttgttc tacacataca tgtcagagta cgctcatcgg 240 gttttctcct ttttgggaaa agacaatgac attctgaccg tcaaagaagc aatcctgggc 300 atccctggac tagaccctat ggatccccac acagctccgg gtttgcccta cgccattagc 360 ggccttgac gtactgatct cgtcgatttt gcgaacggca cggtagaccc ggcactggcc 420 atgcagatcc agaaattctt agacggtgac tactctgatc atgtcttcca aacttttctg 480 aaagatgaaa tcagaccctc agaaaggtc cgggcgggaa aaacccgcat tgtcgatgtg 540 ccctccctgg cgcactgcat tgtgggcaga atgctgcttg ggcgctttgc cgccaagttt 600 caatcccatc ctggctttct ccttggctcc gctatcgggt ctgaccctga tgtcttctgg 660 accgtcatag gggctcagct cgagggaaga aagaacacgt atgacgtgga ctacagtgcc 720 tttgactctt cacacggcac tggctccttc gaggctctca tctctcactt tttcaccgtg 780 gacaatggtt tcagccctgc gctgggaccg tatctcagat ccctggctgt ctcggtgcac 840 gcttacggcg agcgtcgcat caagattacc ggaggcctcc cctctggttg tgccgcgacc 900 agcctgctga acacagtgct caacaatgtg atcatcagga ctgctctggc attgacctac 960 aaggaatttg aatatgacat ggttgatatc atcgcctacg gtgacgacct tctggttggt 1020 acggattatg atctggactt caatgaggtg gcgcggcgcg ctgccaaact ggggtataag 1080 atgactcctg ccaacaaggg ttctgtcttc cctccgactt cctctctctc cgatgctgtt 1140 tttctaaaac gcaaattcgt ccaaaacaat gacggcttat ataaaccagt tatggattta 1200 aagaatttgg aagccatgct ctcctacttc aaaccaggaa cactactcga gaagctgcaa 1260 tctgtttcta tgttggctca acattctgga aaagaagaat acgatagatt gatgcacccc 1320 ttcgctgact atggtgccgt accgagtcac gagtacctgc aggcaagatg gagggccttg 1380 ttcgactga 1389 Description of the Drawings

[0053] Figure 1Shown are the SVVP 13C construct design (A) (SEQ ID NO: 18) and the SVVP 13C-CO (codon-optimized) construct design (B) (SEQ ID NO: 20) for the baculovirus expression system.

[0054] Figure 2 Shown are the SVVP1-His-sIRES-SVV3C construct design (A) (SEQ ID NO: 32) and the SVVP1CO-His-sIRES-SVV3C (codon-optimized) construct design (B) (SEQ ID NO: 33) for the baculovirus expression system.

[0055] Figure 3 Shown are the SVVP13C VP3 / VP1 construct design (A) (SEQ ID NO: 22) of the mutated VP3 / VP1 cleavage site and the SVVP13CD construct design (B) (SEQ ID NO: 24) for the baculovirus expression system.

[0056] Figure 4 Western blots of BaculoFBU / SVVP13C (A) (SEQ ID NO: 18) and BaculoFBU / SVVP13C-CO (B) (SEQ ID NO: 20) supernatant samples compared to native SVV antigens detected with rabbit polyclonal antibodies cross-absorbed with anti-α-SVV VP1, anti-α-SVV VP2, and anti-α-SVV VP3. Lane A is a protein standard, lane 1 is SVV Baculo harvest supernatant, lane 2 is SVV antigen (SEQ ID NO: 3), and lane 3 is a Baculo harvest supernatant negative control. Expected band sizes: full-length SVV P1 = approximately 95 kDa (SEQ ID NO: 19, amino acids 1-859), VP1 = approximately 29 kDa (SEQ ID NO: 19, amino acids 596-859), VP2 - approximately 32 kDa (SEQ ID NO: 19, amino acids 73-356), VP3 = approximately 26 kDa (SEQ ID NO: 19, amino acids 357-595).

[0057] Figure 5Western blots of native SVV virus sucrose fractions (A) and BauloFBU / SVVP13C sucrose fractions (B) using rabbit polyclonal antibodies against α-SVV VP1, α-SVV VP2, and α-SVV VP3 are shown. Lane A is a protein standard, lanes 1-10 are sucrose fractions 1-10, N-BaculoFBU / no insert negative control, P is a positive control native inactivated SVV, and S is the starting sample for the sucrose gradient. Expected band sizes: full-length SVV P1 = approximately 95 kDa (SEQ ID NO: 19, amino acids 1-859), VP1 = approximately 29 kDa (SEQ ID NO: 19, amino acids 596-859), VP2 = approximately 32 kDa (SEQ ID NO: 19, amino acids 73-356), VP3 = approximately 26 kDa (SEQ ID NO: 19, amino acids 357-595).

[0058] Figure 6 Western blots of BaculoFBU / SVVP13C (SEQ ID NO: 18) and BaculoFBU / VVP13C VP3 / VP1 (SEQ ID NO: 22) supernatant harvests using rabbit polyclonal antibodies against α-SVV VP1 (top row) and α-SVV VP3 (bottom row) are shown. Lane A - protein standard, lane 1 - SVV Baculo harvest supernatant, lane 2 - SVV antigen, and lane 3 - Baculo harvest supernatant negative control.

[0059] Figure 7 Western blot of BaculoFBU / VVP13C VP3 / VP1 (SEQ ID NO: 22) sucrose fractions using anti-SVV VP1, anti-SVV VP2, and anti-SVV VP3 rabbit polyclonal antibodies is shown. Lane A is a protein standard, N is a BaculoFBU / no insert native control, P is a positive control native inactivated SVV (SEQ ID NO: 3), S is a SVVP13C VP3 / VP1 (SEQ ID NO: 22) pellet resuspended in TBS, and lanes 1-9 are sucrose fractions 1-9. Full-length SVV P1 = approximately 95 kDa (SEQ ID NO: 19, amino acids 1-859), VP1 = approximately 29 kDa (SEQ ID NO: 19, amino acids 596-859), VP2 - approximately 32 kDa (SEQ ID NO: 19, amino acids 73-356), VP3 = approximately 26 kDa (SEQ ID NO: 19, amino acids 357-595).

[0060] Figure 8Shown is a Western blot of BaculoFBU / VVP13CD (SEQ ID NO: 24) supernatant harvest probed with anti-SVV VP1, anti-SVV VP2, and anti-SVV VP3 rabbit polyclonal antibodies. Lane A is a protein standard, lane 1 is SVV Baculo harvest supernatant, lane 2 is SVV antigen (SEQ ID NO: 3), and lane 3 is a Baculo harvest supernatant negative control. Expected band sizes: full-length SVV P1 = approximately 95 kDa (SEQ ID NO: 19, amino acids 1-859), VP1 = approximately 29 kDa (SEQ ID NO: 19, amino acids 596-859), VP2 = approximately 32 kDa (SEQ ID NO: 19, amino acids 73-356), VP3 = approximately 26 kDa (SEQ ID NO: 19, amino acids 357-595).

[0061] Figure 9 Western blot of BaluloFBU / SVVP13CD (SEQ ID NO: 24) sucrose fractions using anti-SVV VP1, anti-SVV VP2, and anti-SVV VP3 rabbit polyclonal antibodies is shown. Lane A is a protein standard, lane 1 is SVV Baculo harvest supernatant, lane N is a BaculoFBU / no insert negative control, P is a positive control naturally inactivated SVV (SEQ ID NO. 3), S is a SVVP13CD (SEQ ID NO. 25) pellet resuspended in TBA, and lanes 1-10 are sucrose fractions 1-10. Expected band sizes: full-length SVV P1 = approximately 95 kDa (SEQ ID NO: 19, amino acids 1-859), VP1 = approximately 29 kDa (SEQ ID NO: 19, amino acids 596-859), VP2 - approximately 32 kDa (SEQ ID NO: 19, amino acids 73-356), VP3 = approximately 26 kDa (SEQ ID NO: 19, amino acids 357-595).

[0062] Figure 10Western blots of BaculoFBU / SVVP13C (A) (SEQ ID NO: 18), BaculoFBU / SVVP13CD (A) (SEQ ID NO: 24), and BaculoFBU / SVVP13C VP3 / VP1 (B) (SEQ ID NO: 22) are shown. Day 3 soluble fractions were stained with rabbit polyclonal antibodies for α-SVV VP1, α-SVV VP2, and α-SVV VP3. SDS-PAGE gels for BaculoFBU / SVVP13C and BaculoFBU / SVVP13CD (C) are shown. Lane A - protein standard, lane P - positive control native SVV antigen, lane 1 - day 3 soluble fraction SVVP13C, lane 2 - day 3 soluble fraction SVVP13CD, lane 3 - day 3 soluble fraction SVVP13C VP3 / VP1, and lane N - negative control BaculoFBU / no insert soluble fraction. Expected band sizes: full-length SVV P1 = approximately 95 kDa (SEQ ID NO: 19, amino acids 1-859), VP1 = approximately 29 kDa (SEQ ID NO: 19, amino acids 596-859), VP2 - approximately 32 kDa (SEQ ID NO: 19, amino acids 73-356), VP3 = approximately 26 kDa (SEQ ID NO: 19, amino acids 357-595).

[0063] Figure 11 shows a Western blot of sucrose fractions of day 3 soluble BaculoFBU / SVVP13C (SEQ ID NO: 18), BaculoFBU / SVVP13CD (SEQ ID NO: 24), and BaculoFBU / SVVP13C VP3 / VP1 (SEQ ID NO: 22) using anti-α-SVV VP1, anti-α-SVV VP2, and anti-α-SVV VP3 rabbit polyclonal antibodies. Lane A is a protein standard, P is a positive control native inactivated SVV, lanes 1-11 are sucrose fractions 1-11, and N is a negative control BaculoFBU / no insert soluble fraction. Expected band sizes: full-length SVV P1 = approximately 95 kDa (SEQ ID NO: 19, amino acids 1-859), VP1 = approximately 29 kDa (SEQ ID NO: 19, amino acids 596-859), VP2 - approximately 32 kDa (SEQ ID NO: 19, amino acids 73-356), VP3 = approximately 26 kDa (SEQ ID NO: 19, amino acids 357-595).

[0064] Figure 12 Shown are the least mean square rectal temperatures (°C) by study day and group.

[0065] Figure 13 Shown are the group median log values ​​in serum by group and day. 10 Genome copies / mL SVA RNA. Detailed Description of the Invention

[0067] Unless otherwise indicated, the practice of the present invention employs conventional techniques of molecular biology, microbiology, recombinant DNA technology, protein chemistry and immunology, which are within the skill of the art. Such techniques are fully explained in the literature. See, e.g., Sambrook, Fritsch & Maniatis, Molecular Cloning: A Laboratory Manual, Vols. I, II & III, 2nd ed. (1989); DNA Cloning, Vols. I & II (D.N. Glover, ed. 1985); Oligonucleotide Synthesis (M.J. Gait, ed. 1984); Nucleic Acid Hybridization (B.D. Hames & S.J. Higgins, eds. 1984); Animal Cell Culture (R.K. Freshney, ed. 1986); Immobilized Cells and Enzymes (IRL press, 1986); Perbal, B., A Practical Guide to Molecular Cloning (1984); the series, Methods In Enzymology (S. Colowick and N. Kaplan, eds., Academic Press, Inc.); Protein purification methods—a practical approach (E.L.V. Harris and S. Angal, eds., IRL Press at Oxford University Press); and Handbook of Experimental Immunology, Volumes I-IV (DM Weir and CC Blackwell, eds., 1986, Blackwell Scientific Publications).

[0068] Before describing the present invention in detail, it should be understood that the present invention is not limited to specific DNA, polypeptide sequences or process parameters, as these may of course vary. It should also be understood that the terminology used herein is for the purpose of describing specific embodiments of the present invention only and is not intended to be limiting. It must be noted that, as used in this specification and the appended claims, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to an "antigen" includes a mixture of two or more antigens; reference to an "excipient" includes a mixture of two or more excipients, and so on.

[0069] A. Definition

[0070] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art to which the present invention belongs at the time of submission. The meaning and scope of the terms should be clear; however, in the case of any potential ambiguity, the definitions provided herein take precedence over any dictionary or external definition. Further, unless the context requires otherwise, singular terms should include plural terms, and plural terms should include the singular. In this article, unless otherwise stated, the use of "or" means "and / or". In addition, the use of the term "including" and other forms such as "includes" and "included" is not restrictive. All patents and publications mentioned herein are incorporated herein by reference.

[0071] "Protection against disease," "protective immunity," "functional immunity," and similar phrases mean a response to a disease or condition generated by administration of one or more therapeutic compositions of the present invention, or a combination thereof, that results in fewer adverse effects than would be expected in a non-immune subject that has been exposed to the disease or infection. That is, the severity of the adverse effects of the infection is reduced in the vaccinated subject. The infection can be reduced, slowed, or possibly completely prevented in the vaccinated subject. Herein, when complete prevention of infection is intended, it is explicitly stated. If complete prevention is not stated, the term includes partial prevention.

[0072] As used herein, "reduction in the incidence and / or severity of clinical signs" or "reduction in clinical symptoms" means, but is not limited to, reducing the number of infected subjects in a group, reducing or eliminating the number of subjects showing clinical signs of infection, or reducing the severity of any clinical signs present in one or more subjects, compared to wild-type infection. For example, it should refer to any reduction in pathogen load, pathogen shedding, reduction in pathogen transmission, or reduction in any clinical signs or symptoms of malaria. Preferably, these clinical signs are reduced by at least 10% in one or more subjects receiving the therapeutic composition of the present invention, compared to subjects who did not receive the composition and became infected. More preferably, clinical signs are reduced by at least 20%, preferably at least 30%, more preferably at least 40%, and even more preferably at least 50% in subjects receiving the composition of the present invention.

[0073] The term "increased protection" herein means, but is not limited to, a statistically significant reduction in one or more clinical symptoms associated with infection with an infectious agent, preferably SVA, in a group of vaccinated subjects relative to a control group of unvaccinated subjects. The term "statistically significant reduction in clinical symptoms" means, but is not limited to, a frequency of at least 10%, preferably 20%, more preferably 30%, even more preferably 50%, and even more preferably 70% lower occurrence of at least one clinical symptom in a group of vaccinated subjects after challenge with an infectious agent compared to a control group of unvaccinated subjects.

[0074] "Lasting protection" shall mean "improved efficacy" that lasts for at least 3 weeks, but more preferably at least 3 months, still more preferably at least 6 months. In the case of livestock, most preferably the lasting protection shall last until the average age at which the animals are sold for meat.

[0075] An "immunogenic or immunological composition" refers to a composition of matter comprising at least one SVA immunogenic composition or an immunogenic portion thereof, which elicits an immunological response in a host, either a cellular or antibody-mediated immune response to the composition. In a preferred embodiment of the present invention, the immunogenic composition induces an immune response, and more preferably, confers protective immunity against one or more clinical signs of SVA infection.

[0076] As used herein, "immunogenic" or "antigen" refers to a polypeptide or protein that elicits an immune response as described herein. An "immunogenic" SVA protein or polypeptide includes the full-length sequence of any SVA identified herein, or an analog or immunogenic fragment thereof. The term "immunogenic fragment" or "immunogenic portion" refers to a fragment or truncated and / or substituted form of SVA that includes one or more epitopes and thus elicits an immune response as described herein. Generally, such truncated and / or substituted forms or fragments contain at least six contiguous amino acids from the full-length SVA protein. More preferably, the truncated or substituted form or fragment has at least 10, more preferably at least 15, and even more preferably at least 19 contiguous amino acids from the full-length SVA protein. Such fragments can be identified using a variety of epitope mapping techniques well known in the art. See, for example, Epitope Mapping Protocols in Methods in Molecular Biology, Vol. 66 (Glenn E. Morris, ed., 1996), Humana Press, Totowa, New Jersey. For example, linear epitopes can be determined by simultaneously synthesizing a large number of peptides on a solid support, the peptides corresponding to a portion of a protein molecule, and reacting the peptides with antibodies while the peptides are still attached to the support. Such techniques are known and described in the art, see, for example, U.S. Patent No. 4,708,871; Geysen et al. (1984) Proc. Natl. Acad. Sci. USA 81:3998-4002; and Geysen et al. (1986) Molec. Immunol. 23:709-715. Similarly, conformational epitopes are readily identified by determining the spatial conformation of amino acids, for example by x-ray crystallography and two-dimensional nuclear magnetic resonance. See Epitope Mapping Protocols, supra. Synthetic antigens are also included in the definition, such as polytopes, flanking epitopes, and other recombinant or synthetically derived antigens. See, e.g., Bergmann et al. (1993) Eur. J. Immunol. 23:2777-2781; Bergmann et al. (1996) J. Immunol. 157:3242-3249; Suhrbier, A. (1997) Immunol. and Cell Biol. 75:402-408; and Gardner et al. (1998) 12th World AIDS Conference, Geneva, Switzerland, June 28-July 3, 1998. (the teachings and contents of which are all incorporated herein by reference).

[0077] "Immune response" or "immunological response" means, but is not limited to, the development of a cellular and / or antibody-mediated immune response to the composition or vaccine of interest. Typically, an immune response or immunological response includes, but is not limited to, the production or activation of antibodies, B cells, helper T cells, suppressor T cells, and / or cytotoxic T cells specific for one or more antigens included in the composition or vaccine of interest. Preferably, the host exhibits a therapeutic or protective immunological (memory) response such that resistance to new infections is enhanced and / or the clinical severity of the disease is reduced. Such protection is demonstrated by a reduction in the number of symptoms, the severity of symptoms, or the absence of one or more symptoms associated with infection with a pathogen, a delay in the onset of viremia, reduced viral persistence, a reduction in overall viral load, and / or a reduction in viral shedding.

[0078] As used herein, "specific immunoreactivity" refers to an immunoreactive protein or polypeptide that recognizes an antigen that is specific for SVA infection but does not react with an antigen that is specific for a strict challenge control.

[0079] As used herein, "pharmaceutically or veterinarily acceptable carriers" include any and all solvents, dispersion media, coatings, adjuvants, stabilizing agents, diluents, preservatives, antibacterial and antifungal agents, isotonicity agents, adsorption delaying agents, and the like. In some preferred embodiments, and particularly those comprising lyophilized immunogenic compositions, stabilizing agents for use in the present invention include stabilizers for lyophilization or freeze-drying.

[0080] In some embodiments, the immunogenic composition of the present invention contains an adjuvant. As used herein, "adjuvant" can include aluminum hydroxide and aluminum phosphate, saponins such as Quil A, QS-21 (Cambridge Biotech Inc., Cambridge MA), GPI-0100 (Galenica Pharmaceuticals, Inc., Birmingham, AL), water-in-oil emulsions, oil-in-water emulsions, water-in-oil-in-water emulsions. The emulsion can be based in particular on light liquid paraffin oil (European typical); isoprenoid oils, such as squalane or squalene; oils resulting from oligomerization of olefins, particularly isobutylene or decene; esters of acids or alcohols containing linear alkyl groups, more particularly vegetable oils, ethyl oleate, propylene glycol di(caprylate / caprate), triglyceride (caprylate / caprate) or propylene glycol dioleate; esters of branched fatty acids or alcohols, particularly isostearates. The oil is used in combination with an emulsifier to form an emulsion. Emulsifiers are preferably nonionic surfactants, in particular sorbitan, mannide (e.g., mannide oleate), glycols, polyglycerols, propylene glycol and esters of oleic acid, isostearic acid, ricinoleic acid or hydroxystearic acid, which are optionally ethoxylated, and polyoxypropylene-polyoxyethylene copolymer blocks, in particular Pluronic products, especially L 121. See Hunter et al., The Theory and Practical Application of Adjuvants (Ed. Stewart-Tull, DES), John Wiley and Sons, NY, pp. 51-94 (1995), and Todd et al., Vaccine 15: 564-570 (1997). Exemplary adjuvants are the SPT emulsion described on page 147 of "Vaccine Design, The Subunit and Adjuvant Approach", edited by M. Powell and M. Newman, Plenum Press, 1995, and the emulsion MF59 described on page 183 of the same book.

[0081] Further examples of adjuvants are compounds selected from polymers of acrylic acid or methacrylic acid, and copolymers of maleic anhydride and alkenyl derivatives. Advantageous adjuvant compounds are polymers of acrylic acid or methacrylic acid, which are especially cross-linked with polyalkenyl ethers of sugars or polyols. These compounds are known by the term carbomer (Phameuropa, Vol. 8, No. 2, June 1996). Those skilled in the art can also refer to U.S. Patent No. 2,909,462, which describes such acrylic polymers cross-linked with polyhydroxylated compounds having at least 3 hydroxyl groups, preferably no more than 8 hydroxyl groups, the hydrogen atoms of at least three hydroxyl groups being replaced with unsaturated aliphatic radicals having at least 2 carbon atoms. Preferred radicals are those containing 2 to 4 carbon atoms, such as vinyl, allyl and other ethylenically unsaturated groups. The unsaturated radical itself can contain other substituents, such as methyl. The products sold by Lubrizol are particularly suitable. They are crosslinked with allyl sucrose or allyl pentaerythritol. Among them, Carbopol 974P, 934P, and 971P may be mentioned. The use of Carbopol 971P is most preferred. Among the copolymers of maleic anhydride and alkenyl derivatives is the copolymer EMA (Monsanto), which is a copolymer of maleic anhydride and ethylene. Dissolution of these polymers in water results in an acid solution, which is preferably neutralized to physiological pH in order to produce an adjuvant solution into which the immunogenic, immunological, or vaccine composition itself is incorporated.

[0082] Further suitable adjuvants include, but are not limited to, the RIBI adjuvant system (Ribi Inc.), block copolymers (CytRx, Atlanta GA), SAF-M (Chiron, Emeryville CA), monophosphoryl lipid A, avridine lipid-amine adjuvant, heat-labile enterotoxin from Escherichia coli (recombinant or otherwise), cholera toxin, IMS 1314 or muramyl dipeptide, or naturally occurring or recombinant cytokines or analogs thereof or stimulators of endogenous cytokine release, among others.

[0083] It is contemplated that the adjuvant may be added in an amount of about 100 μg to about 10 mg per dose, preferably in an amount of about 100 μg to about 10 mg per dose, more preferably in an amount of about 500 μg to about 5 mg per dose, even more preferably in an amount of about 750 μg to about 2.5 mg per dose, and most preferably in an amount of about 1 mg per dose. Alternatively, the adjuvant may be in a concentration of about 0.01 to 50%, preferably in a concentration of about 2% to 30%, more preferably in a concentration of about 5% to 25%, still more preferably in a concentration of about 7% to 22%, and most preferably in a concentration of 10% to 20% by volume of the final product.

[0084] "Diluents" may include water, saline, dextrose, ethanol, glycerol, etc. Isotonic agents may include, among others, sodium chloride, dextrose, mannitol, sorbitol, and lactose. Stabilizers include, among others, albumin and alkali metal salts of ethylenediaminetetraacetic acid.

[0085] "Isolated" means altered "by the hand of man" from its natural state, i.e., if it occurs in nature, it has been changed or removed from its original environment, or both. For example, a polynucleotide or polypeptide naturally present in a living organism is not "isolated," but the same polynucleotide or polypeptide separated from the coexisting materials of its natural state is "isolated," as the term is employed herein.

[0086] "Safety" refers to the absence of adverse consequences in the vaccinated animal following vaccination, including but not limited to: potential reversion of bacterial-based vaccines to virulence, clinically significant side effects such as persistent systemic disease or unacceptable inflammation at the site of vaccine administration.

[0087] As used herein, the term "vaccination" or "vaccinating" or variations thereof means, but is not limited to, a process comprising administering an immunogenic composition of the present invention which, when administered to an animal, elicits or is capable of eliciting (directly or indirectly) an immune response against SVA in the animal.

[0088] In the context of the present invention, "mortality" refers to death caused by SVA infection and includes situations where the infection is so severe that the animal is euthanized to prevent suffering and provide a humane end to its life.

[0089] As used herein, "effective dose" means, but is not limited to, the amount of an antigen that elicits or is capable of eliciting an immune response that produces a reduction in clinical symptoms in an animal to which the antigen is administered.

[0090] As used herein, the term "effective amount" means, in the context of a composition, the amount of an immunogenic composition capable of inducing an immune response that reduces the incidence of infection or alleviates the severity of infection or the incidence of disease in an animal. In particular, an effective amount refers to colony forming units (CFU) / dose. Alternatively, in the context of treatment, the term "effective amount" refers to a therapeutic amount that is sufficient to reduce or improve the severity or duration of a disease or disorder, or one or more symptoms thereof, prevent the advancement of a disease or disorder, cause the regression of a disease or disorder, prevent the recurrence, development, onset or progression of one or more symptoms associated with a disease or disorder, or enhance or improve the prevention or treatment of another therapy or therapeutic agent.

[0091] The term "fragment" refers to a fragment or truncated and / or substituted form of an SVA peptide or a gene encoding such an SVA peptide that includes one or more epitopes and thus elicits an immune response against SVA. Preferably, such a fragment is a fragment or truncated and / or substituted form of any SVA peptide or any SVA gene provided herein. Generally, such a truncated and / or substituted form or fragment comprises at least six contiguous amino acids from the full-length SVA sequence. More preferably, the truncated or substituted form or fragment has at least 10, more preferably at least 15, and even more preferably at least 19 contiguous amino acids from the full-length SVA sequence. Such fragments can be identified using a variety of epitope mapping techniques well known in the art. See, for example, Epitope Mapping Protocols in Methods in Molecular Biology, Vol. 66 (Glenn E. Morris, ed., 1996), Humana Press, Totowa, New Jersey. For example, linear epitopes can be determined by simultaneously synthesizing a large number of peptides on a solid support, the peptides corresponding to a portion of a protein molecule, and reacting the peptides with antibodies while the peptides are still attached to the support. Such techniques are known and described in the art, see, for example, U.S. Patent No. 4,708,871; Geysen et al. (1984) Proc. Natl. Acad. Sci. USA 81:3998-4002; and Geysen et al. (1986) Molec. Immunol. 23:709-715. Similarly, conformational epitopes are readily identified by determining the spatial conformation of amino acids, for example by x-ray crystallography and two-dimensional nuclear magnetic resonance. See Epitope Mapping Protocols, supra. Synthetic antigens are also included in the definition, such as polytopes, flanking epitopes, and other recombinant or synthetically derived antigens. See, e.g., Bergmann et al. (1993) Eur. J. Immunol. 23:2777-2781; Bergmann et al. (1996) J. Immunol. 157:3242-3249; Suhrbier, A. (1997) Immunol. and Cell Biol. 75:402-408; and Gardner et al. (1998) 12th World AIDS Conference, Geneva, Switzerland, June 28-July 3, 1998. (the teachings and contents of which are all incorporated herein by reference).

[0092] The term "variant" with respect to a sequence (e.g., a polypeptide or nucleic acid sequence) is intended to mean a substantially similar sequence. For nucleotide sequences comprising an open reading frame, variants include those sequences that encode the same amino acid sequence of a native protein due to the degeneracy of the genetic code. Variant nucleotide sequences also include synthetically derived nucleotide sequences, such as those generated by using site-directed mutagenesis, and for open reading frames, encoding native proteins, as well as encoding polypeptides having amino acid substitutions for codon optimization purposes relative to the native protein. Typically, using one of the alignment programs described by standard parameters, the nucleotide sequence variants of the present invention will have at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88% or 89%, more preferably at least 90%, 91%, 92%, 93% or 94%, and most preferably at least 95%, 96%, 96.1%, 96.2%, 96.3%, 96.4%, 96.5%, 96.6%, 96.7%, 96.8%, 96.9%, 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 97.10%, 97.11%, 97.12%, 97.13%, 97.14%, 97.15%, 97.16%, 97.17%, 97.18%, 97.19%, 97.20%, 97.21%, 97.22%, 97.23%, 97.24%, 97.25%, 97.26%, 97.27%, 97.28%, 97.29%, 97.30%, 97.31%, 97.32%, 97.33%, 97.34%, 97.35%, 97.36%, 97.37%, 97.38%, 97.39%, 97.40%, 97.41%, 97.42%, 97.43%, 97.44%, 97.45%, 97.46%, 97.47%, 97.48%, 97.49%, 97.50%, 97.51%, 97 7%, 97.1%, 97.2%, 97.3%, 97.4%, 97.5%, 97.6%, 97.7%, 97.8%, 97.9%, 98%, 98.1%, 98.2%, 98.3%, 98.4%, 98.5%, 98.6%, 98.7%, 98.8%, 98.9%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9% sequence identity.

[0093] As used herein, the term "immunoreactive to SVA" means that the peptide or fragment elicits an immunological response against SVA.

[0094] As used herein, "sequence homology" refers to a method for determining the relatedness of two sequences. To determine sequence homology, two or more sequences are optimally aligned and gaps are introduced when necessary. However, in contrast to "sequence identity", when determining sequence homology, conservative amino acid substitutions are considered to be matches. In other words, in order to obtain a polypeptide or polynucleotide with 95% sequence homology to a reference sequence, 85%, preferably 90%, even more preferably 95% of the amino acid residues or nucleotides in the reference sequence must match or contain conservative substitutions by another amino acid or nucleotide, or up to 15%, preferably up to 10%, even more preferably up to 5% of the total amino acid residues or nucleotides in the reference sequence (excluding conservative substitutions) may be inserted into the reference sequence. Preferably, the homologous sequence comprises a segment of at least 50, even more preferably 100, even more preferably 250, even more preferably 500 nucleotides.

[0095] A "conservative substitution" refers to the replacement of an amino acid residue or nucleotide with another amino acid residue or nucleotide having similar characteristics or properties (including size, hydrophobicity, etc.) such that the overall function is not significantly changed.

[0096] As is known in the art, "sequence identity" refers to the relationship between two or more polypeptide sequences, or two or more polynucleotide sequences, i.e., a reference sequence and a given sequence to be compared with the reference sequence. Sequence identity is determined by comparing a given sequence to a reference sequence after the sequences have been optimally aligned to produce the highest degree of sequence similarity, as determined by matching between strings of such sequences. After such alignment, sequence identity is determined on a position-by-position basis, e.g., if the nucleotide or amino acid residue is the same at a particular position, the sequences are "identical" at that position. The total number of such positional identities is then divided by the total number of nucleotides or residues in the reference sequence to give the % sequence identity. Sequence identity can be readily calculated by known methods, including but not limited to those described in Computational Molecular Biology, Lesk, AN, ed., Oxford University Press, New York (1988), Biocomputing: Informatics and Genome Projects, Smith, DW, ed., Academic Press, New York (1993); Computer Analysis of Sequence Data, Part I, Griffin, AM and Griffin, HG, eds., Humana Press, New Jersey (1994); Sequence Analysis in Molecular Biology, von Heinge, G., Academic Press (1987); Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M. Stockton Press, New York (1991); and Carillo, H. and Lipman, D., SIAM J. Applied Molecular Biology. Math., 48:1073 (1988), which is incorporated herein by reference. The preferred method for determining sequence identity is designed to provide the maximum match between the sequences tested. The method for determining sequence identity is encoded in a publicly available computer program that determines the sequence identity between given sequences. Examples of such programs include, but are not limited to, the GCG program package (Devereux, J. et al., Nucleic Acids Research, 12 (1): 387 (1984)), BLASTP, BLASTN and FASTA (Altschul, SF et al., J. Molec. Biol., 215: 403-410 (1990).The BLASTX program is publicly available from NCBI and other sources (BLASTX Manual, Altschul, S. et al., NCBI NLM NIH Bethesda, MD 20894, Altschul, SF et al., J. Molec. Biol., 215:403-410 (1990), the teachings of which are incorporated herein by reference). These programs use default gap weights to optimally align sequences so as to produce the highest level of sequence identity between a given sequence and a reference sequence. By way of illustration, by a polynucleotide having a nucleotide sequence that has at least, for example, 85%, preferably 90%, even more preferably 95% "sequence identity" to a reference nucleotide sequence, it is meant that the nucleotide sequence of the given polynucleotide is identical to the reference sequence, except that the given polynucleotide sequence may include up to 15, preferably up to 10, even more preferably up to 5 point mutations per every 100 nucleotides of the reference nucleotide sequence. In other words, in a polynucleotide having a nucleotide sequence that is at least 85%, preferably 90%, even more preferably 95% identical to a reference nucleotide sequence, up to 15%, preferably 10%, even more preferably 5% of the nucleotides in the reference sequence may be deleted or substituted with another nucleotide, or multiple nucleotides up to 15%, preferably 10%, even more preferably 5% of the total nucleotides in the reference sequence may be inserted into the reference sequence. These mutations of the reference sequence may occur at the 5' or 3' terminal position of the reference nucleotide sequence, or at any position between those terminal positions, or may be interspersed individually among nucleotides in the reference sequence, or may be interspersed in one or more contiguous groups within the reference sequence. Similarly, by a polypeptide having a given amino acid sequence that has at least, for example, 85%, preferably 90%, even more preferably 95% sequence identity to a reference amino acid sequence, it is meant that the given amino acid sequence of the polypeptide is identical to the reference sequence, except that the given polypeptide sequence may include up to 15, preferably up to 10, even more preferably up to 5 amino acid changes per every 100 amino acids of the reference amino acid sequence. In other words, to obtain a given polypeptide sequence having at least 85%, preferably 90%, even more preferably 95% sequence identity with a reference amino acid sequence, up to 15%, preferably up to 10%, even more preferably up to 5% of the amino acid residues in the reference sequence may be deleted or substituted with another amino acid, or up to 15%, preferably up to 10%, even more preferably up to 5% of the total number of amino acid residues in the reference sequence may be inserted into the reference sequence. These changes in the reference sequence may occur at the amino or carboxyl terminal positions of the reference amino acid sequence, or at any position between those terminal positions, or may be interspersed individually among the residues in the reference sequence, or in one or more contiguous groups within the reference sequence. Preferably, residue positions that are not identical differ by conservative amino acid substitutions.However, conservative substitutions are not included as matches when determining sequence identity.

[0097] The terms "sequence identity" or "percentage identity" are used interchangeably herein. For the purposes of the present invention, as defined herein, in order to determine the percent identity of two amino acid sequences or two nucleic acid sequences, the sequences are aligned for the purpose of optimal comparison (e.g., a gap can be introduced in the first amino acid or nucleic acid sequence for optimal comparison with the second amino acid or nucleic acid sequence). The amino acids or nucleotide residues at corresponding amino acid or nucleotide positions are then compared. When a position in a first sequence is occupied by an amino acid or nucleotide residue identical to the corresponding position in the second sequence, the molecules are identical at that position. The percent identity between two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity = number of identical positions / total number of positions (i.e., overlapping positions) x 100). Preferably, the two sequences are of the same length.

[0098] Can carry out sequence comparison on the whole length of two sequences to be compared or on the fragment of two sequences.And usually and preferably, relatively will carry out on the full length of two sequences to be compared within the scope of the present invention.Yet sequence identity can be carried out on the zone of for example twenty, fifty, one hundred or more adjacent amino acid residues.

[0099] As used herein, it is in particular to be understood that the term "having at least X% sequence identity with the nucleic acid / amino acid sequence according to SEQ ID NO: Y" (or alternatively, the term "having at least X% sequence identity with the nucleic acid / amino acid sequence shown in / of SEQ ID NO: Y") is equivalent to the term "having at least X% sequence identity with the nucleic acid / amino acid sequence according to SEQ ID NO: Y over the length of SEQ ID NO: Y" or the term "having at least X% sequence identity with the nucleic acid / amino acid sequence according to SEQ ID NO: Y over the entire length of SEQ ID NO: Y", respectively.

[0100] Vectors for expression and methods for preparing and / or using vectors (or recombinants) can be by methods disclosed in or similar to methods disclosed in the following: for DNA viral vectors, in particular U.S. Patent Nos. 4,603,112, 4,769,330, 5,174,993, 5,505,941, 5,338,683, 5,494,807, 4,722,848, 5,942,235, 5,364,773, 5,762,938, 5,770,212, 5,942,235, 382,425, PCT Publications WO 94 / 16716, WO 96 / 39491, WO 95 / 30018; Paoletti, "Applications of pox virus vectors to vaccination: An update," PNAS USA 93:11349-11353, October 1996; Moss, "Genetically engineered poxviruses for recombinant gene expression, vaccination, and safety," PNAS USA 93:11341-11348, October 1996; Smith et al., U.S. Patent No. 4,745,051 (recombinant baculovirus); Richardson, CD (ed.), Methods in Molecular Biology 39, "Baculovirus Expression Protocols" (1995 Humana Press Inc.); Smith et al., "Production of Human BetaInterferon in Insect Cells Infected with a Baculovirus Expression Vector," Molecular and Cellular Biology, December 1983, Vol. 3, No. 12, pp. 2156-2165; Pennock et al., "Strong and Regulated Expression of Escherichia coli B-Galactosidase in Infect Cells with a Baculovirus vector," Molecular and Cellular Biology, March 1984, Vol. 4, No. 3, p. 406; EPA 0370 573; U.S. Application No. 920,197, filed October 16, 1986; EP Patent Publication No. 265785; U.S. Patent No. 4,769,331 (recombinant herpes virus); Roizman, "The function of herpes simplex virus genes: A primer for genetic engineering of novel vectors," PNAS USA 93:11307-11312, October 1996; Andreansky et al., "The application of genetically engineered herpes simplex viruses to the treatment of experimental brain tumors," PNAS USA 93:11313-11318, October 1996; Robertson et al., "Epstein-Barr virus vectors for gene delivery to B lymphocytes," PNAS USA 93:11334-11340, October 1996; Frolov et al., "Alphavirus-based expression vectors: Strategies and applications," PNAS USA 93:11371-11377, October 1996; Kitson et al., J. Virol.65, 3068-3075, 1991; U.S. Patent Nos. 5,591,439, 5,552,143; WO 98 / 00166; allowed U.S. application serial Nos. 08 / 675,556 and 08 / 675,566, both filed July 3, 1996 (recombinant adenovirus); Grunhaus et al., 1992, "Adenovirus as cloning vectors," Seminars in Virology (Vol. 3), pp. 237-52, 1993; Ballay et al., EMBO Journal, Vol. 4, pp. 3861-65; Graham, Tibtech 8, 85-87, April 1990; Prevec et al., J. Gen Virol. 70, 42434; PCT WO 91 / 11525; Felgner et al. (1994), J. Biol. Chem.269, 2550-2561, Science, 259: 1745-49, 1993; and McClements et al., "Immunization with DNA vaccines encoding glycoprotein D or glycoprotein B, alone or in combination, induces protective immunity in animal models of herpes simplex virus-2 disease", PNAS USA 93: 11414-11420, October 1996; and U.S. Patent Nos. 5,591,639, 5,589,466 and 5,580,859, and WO 90 / 11092, WO 93 / 19183, WO 94 / 21797, WO 95 / 11307, WO 95 / 20660; Tang et al., Nature, and Furth et al., Analytical Biochemistry. See also WO 98 / 33510; Ju et al., Diabetologia, 41:736-739, 1998 (lentiviral expression system); Sanford et al., U.S. Pat. No. 4,945,050; Fischbach et al. (Intracel); WO 90 / 01543; Robinson et al., Seminars in Immunology, Vol. 9, pp. 271-283 (1997), (DNA vector system); Szoka et al., U.S. Pat. No. 4,394,448 (methods for inserting DNA into living cells); McCormick et al., U.S. Pat. No. 5,677,178 (use of cytopathic viruses); and U.S. Pat. No. 5,928,913 (vectors for gene delivery); and other documents cited herein.

[0101] Preferred viral vectors include baculovirus, such as BaculoGold (BD Biosciences Pharmingen, San Diego, Calif.), particularly when the production cells are insect cells. Although the baculovirus expression system is preferred, it will be appreciated by those skilled in the art that other expression systems may also be used for the purposes of the present invention.

[0102] B. Carrier molecules

[0103] The carrier molecules to which the SVA peptides of the present invention can be conjugated or covalently linked are preferably those described above. Preferred carriers for animal use are bovine serum albumin and keyhole limpet hemocyanin. Protein carriers suitable for human use include tetanus toxoid, diphtheria toxoid, acellular pertussis vaccine (LPF toxoid), and cross-reactive materials (CRMs), which are antigenically similar to bacterial toxins but rendered non-toxic by mutation. For example, CRM 197 obtained according to Pappenheimer et al., Immunochemistry, 9, 891-906 (1972), as well as other bacterial protein carriers, such as meningococcal outer membrane proteins, can be used. Preferably, the carrier protein itself is an immunogen.

[0104] The SVA peptides of the present invention can be covalently coupled to the carrier by any convenient method known in the art. Although the use of symmetrical linkers such as adipic acid dihydrazide as described by Schneerson et al., J. Experimental Medicine, 152, 361-376 (1980), or heterobifunctional linkers such as N-succinimidyl 3-(2-pyridyldithio) propionate as described by Fattom et al., Infection and Immunity, 56, 2292-2298 (1988) is within the scope of the present invention, it is preferred to avoid the use of any linker and instead couple the SVA peptides of the present invention directly to the carrier molecule. Such coupling can be achieved by reductive amination, as described by Landi et al., J. Immunology, 127, 1011-1019 (1981).

[0105] The size of the immunogenic composition, as defined by the average molecular weight, is variable and depends on the SVA peptide chosen and the method of coupling the SVA peptide to the carrier. Thus, it can be as small as 1,000 Daltons (10 3 ) or greater than 10 6 Using the reductive amination coupling method, the molecular weight of the SVA peptide is typically in the range of 5,000 to 500,000, such as 300,000 to 500,000, or such as 5,000 to 50,000 Daltons.

[0106] Carrier molecules that specifically bind to the SVA peptides of the present invention, i.e., peptides, derivatives and analogs thereof, and peptidomimetics, can be produced by various methods known in the art, including, but not limited to, solid phase synthesis or by solution (Nakanishi et al., 1993, Gene 137:51-56; Merrifield, 1963, J. Am. Chem. Soc. 15:2149-2154; Neurath, H. et al., eds., The Proteins, Vol. II, 3rd ed., pp. 105-237, Academic Press, New York, NY (1976), which are incorporated herein by reference in their entireties).

[0107] The SVA peptides of the present invention or the antibodies of the present invention or binding portions thereof can be administered in an injectable dose by solution or suspension in a diluent with a pharmaceutical or veterinary carrier.

[0108] The toxicity and therapeutic efficacy of such molecules can be determined by assaying LD in cell culture or experimental animals, for example. 50 The dose lethal to 50% of the population was determined by standard pharmaceutical procedures.

[0109] The vaccines of the present invention can be multivalent or monovalent. Multivalent vaccines are made by immunoconjugating multiple SVA peptides to carrier molecules.

[0110] In one aspect, the SVA peptide composition comprises an immunogenic conjugate in an effective immunogenic amount, preferably in combination with an immunostimulant; and a physiologically acceptable carrier. As used in this context, "immunostimulant" is intended to encompass any compound or composition that has the ability to enhance the activity of the immune system, whether it is a specific enhancing effect in combination with a specific antigen, or simply an independent effect on the activity of one or more elements of the immune response. Immunostimulant compounds include, but are not limited to, mineral gels, such as aluminum hydroxide; surface active substances, such as lysolecithin, Polyols; polyanions; peptides; oil emulsions; alum and MDP. Methods for utilizing these materials are known in the art, and determining the optimal amount of stimulant for a given vaccine is within the capabilities of the skilled artisan. More than one immunostimulant may be used in a given formulation. The immunogen may also be incorporated into liposomes or conjugated to polysaccharides and / or other polymers for use in vaccine formulations.

[0111] If desired, the composition may be present in a package or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The package may, for example, comprise metal or plastic foil, such as a blister pack. The package or dispenser device may be accompanied by instructions for administration, preferably for administration to mammals, particularly pigs. Associated with such containers may be a notice in a form prescribed by a government agency governing the manufacture, use or sale of a drug or biological product, reflecting approval for human administration by the manufacture, use or sale agency.

[0112] C. Adjuvant

[0113] To further increase the immunogenicity of the immunogenic compositions provided herein and containing one or more SVA peptides, one or more adjuvants may also be included.

[0114] Adjuvants can be purified by any technique previously described or known in the art. Preferred purification techniques are silica gel chromatography, particularly "flash" (fast) chromatography techniques, as described by W. Clark Still et al., J. Organic Chemistry, 43, 2923-2925 (1978). However, other chromatographic methods, including HPLC, can also be used for the purification of adjuvants. Crystallization can also be used to purify adjuvants. In some cases, purification is not required because analytically pure products are obtained directly from synthesis.

[0115] The vaccine composition of the present invention is prepared by physically mixing the adjuvant with the SVA peptide under appropriate sterile conditions according to known techniques to produce an adjuvanted composition. Complexation of the SVA peptide with the adjuvant is facilitated by the presence of a net negative charge on the conjugate, which is electrostatically attracted to the positive charge present on the long chain alkyl compound adjuvant.

[0116] It is contemplated that the adjuvant may be added in an amount of about 100 μg to about 10 mg / dose, preferably in an amount of about 100 μg to about 10 mg / dose, more preferably in an amount of about 500 μg to about 5 mg / dose, even more preferably in an amount of about 750 μg to about 2.5 mg / dose, and most preferably in an amount of about 1 mg / dose. Alternatively, the adjuvant may be in a concentration of about 0.01% to 75%, preferably in a concentration of about 2% to 30%, more preferably in a concentration of about 5% to 25%, still more preferably in a concentration of about 7% to 22%, and most preferably in a concentration of 10% to 20% by volume of the final product.

[0117] D. Physiologically acceptable vehicles

[0118] The vaccine compositions of the present invention can be formulated using techniques similar to those used for other pharmaceutical polypeptide compositions. Thus, the adjuvant and, preferably, the SVA peptide conjugated to a carrier molecule and / or mixed with the adjuvant can be stored in a lyophilized form and reconstituted in a physiologically acceptable vehicle to form a suspension prior to administration. Alternatively, the adjuvant and conjugate can be stored in a vehicle. Preferred vehicles are sterile solutions, particularly sterile buffered solutions such as phosphate-buffered saline. Any method of combining the adjuvant and conjugate in a vehicle that improves the immunological effectiveness of the immunogenic composition is suitable.

[0119] The volume of a single dose of the vaccine of the present invention can vary, but is generally within the range typically employed in conventional vaccines. At the concentrations of the conjugate and adjuvant described above, the volume of a single dose is preferably between about 0.1 ml and about 3 ml, preferably between about 0.2 ml and about 1.5 ml, and more preferably between about 0.2 ml and about 0.5 ml.

[0120] The vaccine compositions of the present invention may be administered by any convenient means.

[0121] E. Preparation ...

Claims

1. A nucleic acid consisting of a nucleotide sequence selected from the group consisting of: (a) a nucleotide sequence selected from SEQ ID NO: 1 and SEQ ID NO: 2; (b) a nucleotide sequence encoding a polypeptide having an amino acid sequence of SEQ ID NO: 3; (c) the complementary strand of any nucleotide sequence of (a) to (b); (d) the DNA equivalent of any nucleotide sequence of (a) to (c); Wherein the nucleic acid encodes Senecavirus A.

2. A Senecavirus A, the genome of which comprises the RNA according to claim 1 (a) to (c).

3. Senecavirus A according to claim 2, wherein the Senecavirus is attenuated or killed.

4. A vector comprising the nucleic acid according to claim 1.

5. A host cell comprising the vector according to claim 4.

6. A polypeptide selected from the following: (a) a polypeptide having an amino acid sequence encoded by the nucleotide sequence of claim 1 (a) to (b); (b) a polypeptide represented by the amino acid sequence of SEQ ID NO: 3; (c) a polypeptide sequence, which is a P1-2A-P3 polypeptide, the amino acid sequence of which is encoded by a nucleotide sequence selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 32, and SEQ ID NO: 33; (d) a polypeptide sequence, which is a P1-2A-P3 polypeptide, the amino acid sequence of the P1-2A-P3 polypeptide being selected from any one of the following: SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, and SEQ ID NO:

29.

7. An immunogenic composition comprising: (a) Senecavirus A according to any one of claims 2 to 3; and / or (b) a nucleic acid according to claim 1; and / or (c) a polypeptide according to claim 6; and / or (e) The polypeptide according to claim 6, which is fused to a heterologous or self-polypeptide; and optionally at least one pharmaceutically acceptable carrier and / or excipient.

8. A vaccine comprising: (a) Senecavirus A according to any one of claims 2 to 3; and / or (b) a nucleic acid according to claim 1; and / or (c) a polypeptide according to claim 6; and / or (e) The polypeptide according to claim 6, which is fused to a heterologous or self-polypeptide; and at least one pharmaceutically acceptable carrier and / or excipient; Optionally, the vaccine further comprises at least one adjuvant.

9. The vaccine according to claim 8, wherein the vaccine is a recombinant vaccine or a killed vaccine.

10. (a) Senecavirus A according to any one of claims 2 to 3; and / or (b) a nucleic acid according to claim 1; and / or (c) a polypeptide according to claim 6; and / or (e) a polypeptide according to claim 6, fused to a heterologous or autologous polypeptide in the preparation of an immunogenic composition or vaccine for the prevention and / or treatment of a subject against Senecavirus A infection, or for eliciting an immune response against Senecavirus A infection in a subject.

11. Use of (a) Senecavirus A according to any one of claims 2 to 3; and / or (b) a nucleic acid according to claim 1; and / or (c) a polypeptide according to claim 6; and / or (e) a polypeptide according to claim 6 fused to a heterologous or autologous polypeptide in the preparation of an immunogenic composition or vaccine for reducing the incidence or severity of one or more clinical signs associated with or caused by Senecavirus A infection in a subject, the clinical signs being selected from: idiopathic blistering disease; coronary band ulcers and hoof loss; and breeding females showing sudden cessation of feeding, lethargy, anorexia and / or fever up to 105° Fahrenheit.

12. The use according to claim 10 or 11, wherein the subject is a pig.

13. A kit for vaccinating a subject against a disease associated with Senecavirus A infection, and / or reducing the incidence or severity of one or more clinical signs associated with or caused by Senecavirus A infection in a subject, the kit comprising: (a) a dispenser capable of administering the vaccine to the subject; and (b) an immunogenic composition according to claim 7 or a vaccine according to claim 8 or 9, and (c) Optional instruction manual.

14. The kit according to claim 13, wherein the subject is a pig.

15. Use of one or more oligonucleotide primers and / or probes specific for nucleic acids according to claim 1 in the preparation of a kit for the method of detecting Senecavirus A in a sample according to claim 2 or 3, the method comprising the following steps: (i) contacting the sample with one or more oligonucleotide primers and / or probes specific for a nucleic acid according to claim 1, and (ii) detecting binding between the Senecavirus A and the one or more oligonucleotide primers and / or probes; The presence of Senecavirus A indicates infection.

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