A platelet aggregation detection device based on the principle of bioelectrical impedance

Abstract

The present invention relates to a platelet aggregation detection device based on the principle of bioelectrical impedance, which includes a constant-temperature container, a piezoelectric pump, an ultrasonic flowmeter, a dragon fruit controller, a microchannel, and a signal processing circuit, and is externally connected to a signal receiver, a PC, and a mobile device. The constant-temperature container is used to store platelets at a constant temperature; the piezoelectric pump is used to transport platelets; the ultrasonic flowmeter is used to detect the platelet flow rate; the dragon fruit controller is used to generate and receive electrical signals; the microchannel is used to obtain the impedance values of platelets in different states in the flow channel; the signal processing circuit is used to process electrical signals and convert electrical parameters. The advantages are as follows: First, it is integrated into a miniaturized detection device, and the device realizes portability; second, it realizes dynamic detection, making up for the deficiencies of static detection methods; third, it uses the principle of bioelectrical impedance to detect and analyzes data with multiple parameters, improving the reliability of data and can be applied to the field of biological medicine.

Description

Technical Field

[0001] The present invention relates to the field of medical detection, and particularly to a platelet aggregation detection device based on the principle of bioelectrical impedance. Background Art

[0002] The prevention and treatment of cardiovascular diseases in the elderly has become an important global issue. Cardiovascular diseases have become one of the important diseases seriously threatening the health of the elderly population, and have always ranked at the top of all-cause deaths in recent epidemiological surveys. With the increase in age, the cardiovascular system will undergo corresponding physiological changes. After the elderly get sick, the pathophysiological changes of the body are different from those of other age groups. The body changes of the elderly include psychological changes, which have a significant impact on the clinical manifestations, prevention and treatment, and prognosis of diseases. Therefore, the treatment and prevention of cardiovascular diseases in the elderly have their own characteristics, and the treatment countermeasures are also different from those of other age groups, even quite different, either with emphasis or completely different. Preventing, diagnosing, and treating elderly diseases are important issues we face. Studies have shown that most cardiovascular diseases have influencing factors of platelet aggregation. Platelet aggregation is more accurately described as platelet cohesion. Platelet cohesion is the process in which platelets adhere to each other at the site of vascular injury. Platelets play a key role in the development of thrombosis. At the same time, the degree of platelet cohesion is also an important indicator for detecting cardiovascular diseases. Therefore, a method for preventing or diagnosing cardiovascular diseases by detecting the degree of platelet cohesion is proposed to solve the problems brought about by population aging.

[0003] Platelet aggregation is one of the main functions of platelets. When the number and function of platelets are abnormal, it will cause obstacles to the hemostasis mechanism. Platelet aggregation is an important factor in the formation of pathological thrombi to a certain extent, and platelet aggregation plays a key role in the formation process of many pathological thrombi. The detection of platelet aggregation function is beneficial to timely detect whether there is a risk of thrombus formation, and it is also of great significance in clinical aspects for detecting the dosage of antiplatelet drugs and the like.

[0004] At present, the turbidimetry method is a commonly used method in clinical and research in recent years. However, there are some disadvantages in measuring the degree of platelet aggregation, such as being insensitive to the formation of platelet aggregates, only being able to detect large platelet aggregates, the PRP of hyperlipidemia will affect the light transmittance, and reducing the difference between PRP and PPP. The shear-induced platelet aggregation assay has also received attention from the medical community at present, but the detection method has not been proposed. In addition, there are also scattered particle detection methods, platelet counting methods, microtiter plate methods, etc., which generally have the defects of long detection time, complex operation, and being greatly affected by external factors.

[0005] In summary, the present invention proposes a platelet aggregation detection device based on the principle of bioelectrical impedance. By using a microchannel structure and a bioelectrical impedance detection method, the change in impedance value under the normal flow state and the aggregation state of platelets is detected. According to the numerical calculation, the change in multi-parameter values is obtained, and this change value is analyzed to obtain the influence caused by platelet aggregation. The present invention will achieve dynamic detection, making the experimental conclusion have a certain reliability. The invention is committed to researching a portable and household detection device to alleviate problems such as serious cardiovascular diseases faced by the aging population. Summary of the Invention

[0006] The object of the present invention is to provide a platelet aggregation detection device based on the principle of bioelectrical impedance.

[0007] The present invention realizes a portable and household structural design, which is mainly applied to the detection of thrombus formation and the monitoring of the dosage of antiplatelet drugs in the clinical aspect.

[0008] The technical problems to be solved by the present invention are as follows: The data obtained by adding platelet agonists in a static state are not sufficient to reflect the platelet parameters in the normal flow state; the current devices for detecting platelet aggregation have not achieved portability and miniaturization; there are large errors in single-parameter analysis.

[0009] To solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0010] The port a of the constant temperature container (1) is connected to the port c of the piezoelectric pump (2), the port d of the piezoelectric pump (2) is connected to the port e of the ultrasonic flowmeter (3), the port f of the ultrasonic flowmeter (3) is connected to the port g of the microchannel (4), the port h of the microchannel (4) is connected to the port b of the constant temperature container (1). In the microchannel (4), the first electrode e1 is connected to the signal processing circuit I (6) and then connected to the first port In1 of the pitaya controller, the second electrode e2 is connected to the signal processing circuit I (6) and then connected to the second port In2 of the pitaya controller, the third electrode e3 is connected to the signal processing circuit II (7) and then connected to the third port Out1 of the pitaya controller, the fourth electrode e4 is connected to the signal processing circuit II (7) and then connected to the fourth port Out2 of the pitaya controller. The output port i of the pitaya controller (5) is connected to the port j of the signal receiver (8), the port k of the signal receiver (8) is wired to the port l of the PC, and the signal receiver (8) transmits wireless signals to the mobile device.

[0011] The constant-temperature container (1) is used to store platelets at a constant temperature. The piezoelectric pump (2) transports platelets, and the ultrasonic flowmeter (3) detects the platelet flow rate. The pitaya controller (5) generates and receives electrical signals, obtains the impedance values of platelets in different states in the microchannel (4), and transmits the impedance values to the pitaya controller (5) in the form of electrical signals, then to the signal receiver (8), and then feeds back to the PC or mobile device for numerical calculation, curve plotting, and linear fitting analysis.

[0012] The advantages of the present invention are as follows: First, it is integrated into a miniaturized detection device, allowing patients to perform diagnostic tests at home without having to visit the hospital frequently, realizing portability of the device; Second, it realizes dynamic detection, making up for the deficiencies of static detection methods; Third, it uses the principle of bioelectrical impedance to detect, analyzes data with multiple parameters, and simultaneously establishes a microscopic coupling model for simulation and fitting, improving the reliability of the data. Brief Description of the Drawings

[0013] Figure 1 is a schematic diagram of the overall framework structure of the present invention;

[0014] Figure 2 is a schematic diagram of the overall three-dimensional structure of the present invention;

[0015] Figure 3 is an oblique two-axis axonometric view of the microchannel of the present invention. Detailed Embodiment

[0016] Refer to Figures 1 to 3 to specifically describe this embodiment. A platelet aggregation detection device described in this embodiment.

[0017] The port a of the constant-temperature container (1) is connected to the port c of the piezoelectric pump (2). The port d of the piezoelectric pump (2) is connected to the port e of the ultrasonic flowmeter (3). The port f of the ultrasonic flowmeter (3) is connected to the port g of the microchannel (4). The port h of the microchannel (4) is connected to the port b of the constant-temperature container (1). The electrode e1 in the microchannel (4) is connected to the signal processing circuit I (6) and then to the port In1 of the pitaya controller. The electrode e2 is connected to the signal processing circuit I (6) and then to the port In2 of the pitaya controller. The electrode e3 is connected to the signal processing circuit II (7) and then to the port Out1 of the pitaya controller. The electrode e4 is connected to the signal processing circuit II (7) and then to the port Out2 of the pitaya controller. The output port i of the pitaya controller (5) is connected to the port j of the signal receiver (8). The port k of the signal receiver (8) is wired to the port l of the PC. The signal receiver (8) transmits to the mobile device through wireless signals.

[0018] Add an appropriate amount of platelet-rich plasma solution to the constant-temperature container (1). Apply electricity to heat the electrode plates to 37 °C and maintain a small fluctuation around this temperature. Apply a constant voltage signal to the piezoelectric pump (2) to transport platelets from the constant-temperature container (1) into the pipeline and achieve stable flow. At the same time, turn on the ultrasonic flowmeter (3) to detect the flow rate to match the flow velocity in the normal blood flow state. The pitaya controller (5) is turned on and generates an electrical signal that is transmitted to the electrodes in the microchannel (4). When platelets pass through the microchannel (4), the four-electrode method is applied for analysis. Through signal processing by the signal processing circuit I (6), a voltage is applied to electrode one e1 and electrode two e2, and electrode three e3 and electrode four e4 are used to measure the current. The generated electrical signal is transmitted through the signal processing circuit II (7) into the pitaya controller (5). After data processing, the impedance value is obtained. The obtained impedance value is transmitted to the signal receiver (8) in the form of an electrical signal, transmitted to the mobile device (10) through the wireless end, and transmitted to the PC (9) through the wired end; Add an appropriate amount of ADP or adrenaline to the constant-temperature container (1) to activate and start aggregating platelets. After another cycle, the current value obtained in the microchannel (4) is transmitted to the pitaya controller (5) in the form of an electrical signal through the signal processing circuit II (7). After data processing, the impedance value is obtained. The obtained impedance value is transmitted to the signal receiver (8) in the form of an electrical signal, transmitted to the mobile device (10) through the wireless end, and transmitted to the PC (9) through the wired end; A curve graph is obtained in the mobile device (10) or the PC (9). Through numerical calculation and curve fitting, variation values of parameters such as impedance, characteristic frequency, and dielectric constant are obtained. This variation value is considered to be the influence generated by platelet aggregation.

Claims

1. A platelet aggregation measurement device based on the principle of bioelectrical impedance includes a thermostatic container (1), a piezoelectric pump (2), an ultrasonic flowmeter (3), a pitaya controller (5), a microchannel (4), a signal processing circuit I (6), a signal processing circuit II (7), an external signal receiver (8), a PC (9), and a mobile device (10); the thermostatic container (1), the piezoelectric pump (2), the ultrasonic flowmeter (3), the pitaya controller (5), the microchannel (4), the signal processing circuit I (6), and the signal processing circuit II (7) form a device; port a of the thermostatic container (1) is connected to port c of the piezoelectric pump (2), port d of the piezoelectric pump (2) is connected to port e of the ultrasonic flowmeter (3), port f of the ultrasonic flowmeter (3) is connected to port g of the microchannel (4), port h of the microchannel (4) is connected to port b of the thermostatic container (1), electrode e1 in the microchannel (4) is connected to the signal processing circuit I (6) and then to port In1 of the pitaya controller, electrode e2 is connected to the signal processing circuit I (6) and then to port In2 of the pitaya controller, electrode e3 is connected to the signal processing circuit II (7) and then to port Out1 of the pitaya controller, electrode e4 is connected to the signal processing circuit II (7) and then to port Out2 of the pitaya controller, the output port i of the pitaya controller (5) is connected to port j of the signal receiver (8), port k of the signal receiver (8) is wired to port l of the PC, and the signal receiver (8) transmits to the mobile device through a wireless signal; Add an appropriate amount of platelet-rich plasma solution to the constant-temperature container (1). Apply electricity to heat the electrode plates to 37 °C and maintain a small fluctuation around this temperature. Apply a constant voltage signal to the piezoelectric pump (2) to transport platelets from the constant-temperature container (1) into the pipeline and achieve stable flow. At the same time, turn on the ultrasonic flowmeter (3) to detect the flow rate to match the flow rate in the normal blood flow state. The dragon fruit controller (5) is turned on and generates an electrical signal that is transmitted to the electrodes of the microchannel (4). When platelets pass through the microchannel (4), the four-electrode method is used for analysis. Through signal processing by the signal processing circuit I (6), a voltage is applied to electrode one e1 and electrode two e2, and electrode three e3 and electrode four e4 are used to measure the current. The generated electrical signal is transmitted to the dragon fruit controller (5) through the signal processing circuit II (7). After data processing, the impedance value is obtained. The obtained impedance value is transmitted to the signal receiver (8) in the form of an electrical signal, transmitted to the mobile device (10) through the wireless end, and transmitted to the PC (9) through the wired end; Add an appropriate amount of ADP or adrenaline to the constant-temperature container (1) to activate platelet aggregation. After another cycle, the current value obtained in the microchannel (4) is transmitted to the dragon fruit controller (5) in the form of an electrical signal through the signal processing circuit II (7). After data processing, the impedance value is obtained. The obtained impedance value is transmitted to the signal receiver (8) in the form of an electrical signal, transmitted to the mobile device (10) through the wireless end, and transmitted to the PC (9) through the wired end; A curve graph is obtained in the mobile device (10) or PC (9). Through numerical calculation and curve fitting, the change values of the parameters impedance, characteristic frequency, and dielectric constant are obtained, and this change value is considered to be the influence caused by platelet aggregation.

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