A fully automatic sample pretreatment device

Abstract

The application belongs to the field of mechanical devices and provides a full-automatic sample pretreatment device, which comprises a weighing device for weighing the weight of a sample cup after a sample is added; a liquid adding device for adding a buffer solution into the sample cup; an oscillation and transfer device for oscillating and transferring the sample cup; a conveying device for moving a cuvette; a pipetting device for extracting liquid in the sample cup after oscillation and injecting the liquid into the cuvette; and a cleaning device for cleaning a needle of the pipetting device. The full-automatic sample pretreatment device provided by the application realizes linkage of data and operations between steps, automatically completes the steps of sample pretreatment before sampling, is easy to use, avoids manual operation errors, saves labor cost, improves work efficiency, and facilitates user control, use and maintenance of the instrument through mechatronics.

Description

Technical Field

[0001] This invention relates to a mechanical device, and more particularly to a fully automated sample pretreatment device. Background Technology

[0002] In recent years, with the improvement of people's living standards, people have paid more and more attention to healthy eating, and the market has become increasingly strict in controlling the circulation of fruits and vegetables, leading to a more sophisticated testing industry. However, sample pretreatment still relies on manual extraction, resulting in increasingly high labor costs for companies. Existing traditional testing equipment cannot replace manual pretreatment; it still requires manual completion of a series of steps such as weighing, adding, shaking, and extracting the sample. Furthermore, human error is inevitable in each stage of sample pretreatment, directly leading to inaccurate liquid extraction and ultimately affecting the test results. Summary of the Invention

[0003] The purpose of this invention is to overcome the existing defects and provide a fully automated sample pretreatment device that solves the problems of high labor costs and inaccurate liquid extraction caused by human operation errors.

[0004] To solve the above-mentioned technical problems, the present invention provides the following technical solution:

[0005] A fully automated sample pretreatment device includes a weighing device for weighing the sample cup after adding the sample; a liquid addition device for adding buffer solution to the sample cup; a shaking and transferring device for shaking and transferring the sample cup; a conveying device for moving the cuvette; a pipetting device for extracting the liquid from the shaken sample cup and injecting it into the cuvette; and a cleaning device for cleaning the needle of the pipetting device.

[0006] Furthermore, including a worktable, the oscillating transmission device includes several fixed columns, the lower ends of which are fixedly connected to the worktable, and the upper ends of which are connected to a fixed platform. A first drive motor is provided on the lower end face of the fixed platform, and a first coupling is provided on the fixed platform. The lower end of the first coupling is connected to the motor shaft of the first drive motor, and the upper end of the first coupling is connected to a first rotating rod. The upper end of the first rotating rod is connected to a loading tray, and the edge of the loading tray is provided with several placement holes.

[0007] Furthermore, the weighing device includes several guide columns, the lower ends of which are fixedly connected to the worktable. Each guide column has a guide sleeve fitted onto its upper end. The upper ends of the guide sleeves are connected to a lifting platform. The lower end face of the lifting platform is connected to a threaded sleeve. A first screw is threadedly connected to the threaded sleeve. The lower end of the first screw is connected to a second coupling. A second drive motor is disposed below the lifting platform on the upper end face of the worktable. The motor shaft of the second drive motor is connected to the second coupling. A fixed seat is disposed on the upper end face of the lifting platform. A weighing sensor is connected to the upper end of the fixed seat. A weighing pan is connected to the upper end of the weighing sensor. The weighing pan is located directly below any of the placement holes.

[0008] Furthermore, the liquid dispensing device includes a base, the lower end of which is fixedly connected to the workbench, and a bracket connected to the upper end of the base. A first sliding arm is vertically connected to the upper end of the bracket, and a liquid dispensing needle is connected to the front end of the first sliding arm. The liquid dispensing needle is connected to a first peristaltic pump through a first pipeline. The first peristaltic pump is connected to a third drive motor and a buffer solution container. The liquid dispensing needle is located directly above any of the placement holes.

[0009] Furthermore, the support is a hollow support, and the upper and lower ends of the hollow support are provided with pipe holes, and the middle section of the first pipe is located inside the hollow support.

[0010] Furthermore, the pipetting device includes two vertically fixed plates arranged opposite each other. The lower ends of the two vertically fixed plates are fixedly connected to the worktable. Vertical slide rails are provided on the opposite inner surfaces of the two vertically fixed plates. A support plate is slidably connected between the two vertical slide rails. A second screw is threadedly connected to the support plate. A fourth drive motor is provided below the support plate on the upper surface of the worktable. The motor shaft of the fourth drive motor is connected to the second screw via a third coupling. A driven wheel is rotatably connected to the upper surface of the support plate. A second rotating rod is fixedly connected to the upper end of the driven wheel. A second sliding arm is vertically connected to the upper end of the second rotating rod. A sampling needle is connected to the front end of the second sliding arm. A fifth drive motor is fixedly connected to the lower surface of the support plate. The upper end of the motor shaft of the fifth drive motor passes through the support plate and is fixedly connected to a driving wheel. The driving wheel is connected to the driven wheel via a first synchronous belt. A second peristaltic pump is connected to the sampling needle via a second pipeline. The second peristaltic pump is connected to a sixth drive motor and a water container.

[0011] Furthermore, the upper ends of the two vertical fixing plates are connected to a guide plate, the guide plate is provided with a guide hole, the rotating rod passes through the guide hole, and the rotating rod is slidably connected to the guide hole.

[0012] Furthermore, a positioning disk is fitted on the outer side of the rotating rod, a first optocoupler is provided on the side of the positioning disk, and a second optocoupler is provided on the lower end face of the tray.

[0013] Furthermore, the conveying device includes a fixed frame, the lower end of which is fixedly connected to the worktable, and the upper end of which is provided with a guide rail groove. A cuvette holder is provided in the guide rail groove and is slidably connected to the guide rail groove. A movable push plate is connected to either end of the cuvette holder. A guide rail is horizontally provided on the front end face of the fixed frame, and a slider is slidably connected to the guide rail. The upper end of the slider is fixedly connected to the movable push plate. A synchronous pulley is provided at the left and right ends of the front end face of the fixed frame below the guide rail. A second synchronous belt is provided between the two synchronous pulleys. The upper section of the second synchronous belt is fixedly connected to the lower end of the slider. A seventh drive motor is connected to either synchronous pulley and is installed inside the fixed frame.

[0014] Furthermore, the cleaning device includes a support base, the lower end of which is fixedly connected to the workbench, and the upper end of which is connected to a needle washing tank. A liquid inlet is located in the center of the needle washing tank, and the liquid inlet is connected to a liquid inlet. The liquid inlet is connected to a third peristaltic pump via a third pipeline. The third peristaltic pump is connected to an eighth drive motor and a cleaning fluid container. An annular liquid outlet is located around the liquid inlet inside the needle washing tank, and the liquid outlet is connected to a liquid outlet. The liquid outlet is connected to a waste liquid container via a fourth pipeline.

[0015] This invention provides a fully automatic sample pretreatment device that achieves data and operation linkage between each step, automatically completes the sample pretreatment steps, is easy to use, avoids human operation errors, saves labor costs, improves work efficiency, and its mechatronics integration facilitates user control, use and maintenance of the instrument. Attached Figure Description

[0016] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0017] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2 This is a schematic diagram of the oscillation transmission device in this invention;

[0019] Figure 3 This is a schematic diagram of the weighing device in this invention;

[0020] Figure 4 This is a schematic diagram of the liquid addition device in this invention;

[0021] Figure 5 This is a schematic diagram of the main structure of the pipetting device in this invention;

[0022] Figure 6 This is a top view of the pipetting device in this invention;

[0023] Figure 7 This is a schematic diagram of the conveying device in this invention;

[0024] Figure 8 This is a schematic diagram of the cleaning device in this invention. Detailed Implementation

[0025] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0026] like Figure 1-8 As shown, a fully automated sample pretreatment device includes a weighing device 1 for weighing the sample cup after adding the sample; a liquid adding device 2 for adding buffer solution to the sample cup; a shaking and transferring device 3 for shaking and transferring the sample cup; a conveying device 4 for moving the cuvette; a pipetting device 5 for extracting the liquid in the shaken sample cup and injecting it into the cuvette; and a cleaning device 6 for cleaning the needle of the pipetting device.

[0027] The device also includes a worktable 7, and the oscillating conveying device 3 includes several fixed columns 31. The lower ends of the fixed columns 31 are fixedly connected to the worktable 7, and the upper ends of the fixed columns 31 are connected to a fixed platform 32. A first drive motor 33 is provided on the lower end face of the fixed platform 32, and a first coupling is provided on the fixed platform 32. The lower end of the first coupling is connected to the motor shaft of the first drive motor 33, and the upper end of the first coupling is connected to a first rotating rod 34. The upper end of the first rotating rod 34 is connected to a loading tray 35, and the edge of the loading tray 35 is provided with several placement holes.

[0028] The weighing device 1 includes several guide columns 11. The lower end of the guide column 11 is fixedly connected to the workbench 7. Each guide column 11 has a guide sleeve 12 sleeved on its upper end. The upper end of the guide sleeve 12 is connected to a lifting platform 13. The lower end face of the lifting platform 13 is connected to a threaded sleeve. The threaded sleeve is internally threaded to a first screw 14. The lower end of the first screw 14 is connected to a second coupling. The upper end face of the workbench 7 is provided with a second drive motor 15 below the lifting platform 13. The motor shaft of the second drive motor 15 is connected to the second coupling. The upper end face of the lifting platform 13 is provided with a fixed seat 16. The upper end of the fixed seat 16 is connected to a weighing sensor 17. The upper end of the weighing sensor 17 is connected to a weighing pan 18. The weighing pan 18 is located directly below any placement hole.

[0029] The liquid dispensing device 2 includes a base 21, the lower end of which is fixedly connected to the workbench 7. A bracket 22 is connected to the upper end of the base 21. A first sliding arm 23 is vertically connected to the upper end of the bracket 22. A liquid dispensing needle 24 is connected to the front end of the first sliding arm 23. The liquid dispensing needle 24 is connected to a first peristaltic pump through a first pipeline. The first peristaltic pump is connected to a third drive motor and a buffer solution container. The liquid dispensing needle 24 is located directly above any placement hole.

[0030] The pipetting device 5 includes two vertically fixed plates 51 arranged opposite each other. The lower ends of the two vertically fixed plates 51 are fixedly connected to the worktable 7. Vertical slide rails are provided on the opposite inner surfaces of the two vertically fixed plates 51. A support plate 52 is slidably connected between the two vertical slide rails. A second screw is threadedly connected to the support plate 52. A fourth drive motor 53 is provided on the upper surface of the worktable 7 below the support plate 52. The motor shaft of the fourth drive motor 53 is connected to the second screw through a third coupling. A driven wheel 54 is rotatably connected to the upper surface of the support plate 52. A second rotating rod 55 is fixedly connected to the upper end of 54. A second sliding arm 56 is vertically connected to the upper end of the second rotating rod 55. A sampling needle 57 is connected to the front end of the second sliding arm 56. A fifth drive motor 58 is fixedly connected to the lower end face of the tray 52. ​​The upper end of the motor shaft of the fifth drive motor 58 passes through the tray 52 and is fixedly connected to the drive wheel 59. The drive wheel 59 is connected to the driven wheel 54 through a first synchronous belt. A second peristaltic pump is connected to the sampling needle 57 through a second pipeline. The second peristaltic pump is connected to a sixth drive motor and a clean water container.

[0031] The conveying device 4 includes a fixed frame 41, the lower end of which is fixedly connected to the worktable 7. The upper end of the fixed frame 41 is provided with a guide rail groove 42, and a cuvette holder 43 is provided in the guide rail groove 42. The cuvette holder 43 is slidably connected to the guide rail groove 42. A movable push plate 44 is connected to either end of the cuvette holder 43. A guide rail 45 is horizontally provided on the front end face of the fixed frame 41. A slider 46 is slidably connected on the guide rail 45. The upper end of the slider 46 is fixedly connected to the movable push plate 44. A synchronous wheel 47 is provided at the left and right ends of the front end face of the fixed frame 41 below the guide rail 45. A second synchronous belt is provided between the two synchronous wheels 47. The upper section of the second synchronous belt is fixedly connected to the lower end of the slider 46. A seventh drive motor is connected to either synchronous wheel 47. The seventh drive motor is installed inside the fixed frame 41.

[0032] The cleaning device 6 includes a support base, the lower end of which is fixedly connected to the workbench 7. The upper end of the support base is connected to a needle washing tank. A liquid inlet 71 is provided in the center of the needle washing tank. The liquid inlet 71 is connected to a liquid inlet 72. The liquid inlet 72 is connected to a third peristaltic pump through a third pipeline. The third peristaltic pump is connected to an eighth drive motor and a cleaning fluid container. An annular liquid outlet 73 is provided around the liquid inlet 71 inside the needle washing tank. The liquid outlet 73 is connected to a liquid outlet. The liquid outlet is connected to a waste liquid container through a fourth pipeline.

[0033] The working principle includes the following steps:

[0034] 1. Place the sample into the sample cup, and then place the sample cup into the placement hole above the weighing pan. At this time, the weighing pan rises to its highest point, and the sample cup will fall onto the weighing pan, thus obtaining the weight of the sample and the sample cup. The processor records the position and weight of the sample cup. Then the weighing pan descends, the carrying plate rotates to remove the sample cup and move the adjacent placement hole that does not contain a sample cup to the top of the weighing pan. The weighing pan resets, and the above process is repeated until there is a sample cup in each placement hole.

[0035] 2. The liquid addition device adds a buffer solution matching its weight to each sample cup according to the position and weight information recorded by the processor and in coordination with the rotation of the sample tray (in this embodiment, the buffer solution is used to wash out pesticide residues on the surface of vegetables and fruits). After all the sample cups have been filled with buffer solution, the sample tray rotates back and forth to make the liquid inside the sample cups vibrate and mix evenly.

[0036] 3. Place the cuvettes in the cuvette holder. After shaking, rotate the second rotating rod to bring the sampling needle above the sample cup. The tray lowers to allow the sampling needle to insert into the sample cup for sampling. After sampling, the tray rises, and the second rotating rod rotates to bring the sampling needle above the cuvette furthest from the moving push rod. The tray lowers to allow the sampling needle to insert into the cuvette and inject the sampled liquid into it. Then, the tray rises, and the second rotating rod rotates to bring the sampling needle above the needle washing tank. The tray lowers to allow the sampling needle to insert into the needle washing tank. The second peristaltic pump operates to clean the inside of the sampling needle with clean water. The third peristaltic pump operates to clean the inside of the needle. The outside of the sampling needle is cleaned with liquid. After the liquid level in the inlet tank rises, the waste liquid enters the outlet tank and is finally discharged into the waste liquid collection container. Then, the tray rises, and the second rotating rod rotates to return the sampling needle to the top of the sample cup where it was initially sampled. During this process, the carrying tray rotates, so that the adjacent unsampled sample cups reach the position of the previously sampled sample cups. At the same time, the push plate moves, pushing the cuvette holder so that the adjacent cuvettes that have not been filled with sampling liquid reach the position of the cuvettes that have been filled with sampling liquid. The above process is repeated until the liquid in each sample cup has been sampled, thus completing the entire pre-sampling treatment.

[0037] It should be noted that this device is equipped with a power supply for powering the device and a processor for processing data and transmitting signals. The power supply and processor are respectively connected to the weighing device, the liquid adding device, the oscillating conveying device, the conveying device, the pipetting device, and the cleaning device to enable each component to operate according to the above principles. The control process is all existing technology.

[0038] The support 22 is a hollow support with pipe holes at both the upper and lower ends. The middle section of the first pipe is located inside the hollow support, which protects the first pipe while saving space.

[0039] The upper ends of the two vertical fixed plates 51 are connected to a guide plate 510. The guide plate 510 is provided with a guide hole. The rotating rod passes through the guide hole and the rotating rod 55 is slidably connected to the guide hole, so that the lifting and rotation of the rotating rod is more stable.

[0040] A positioning plate is fitted onto the outer side of the rotating rod 55. A first optocoupler is located beside the positioning plate, and a second optocoupler is located on the lower end face of the support plate 52. The first optocoupler is used to sense the displacement of the positioning plate, and the second optocoupler is used to sense the displacement of the support plate, making their movement more precise.

[0041] This invention provides a fully automatic sample pretreatment device that achieves data and operation linkage between each step, automatically completes the sample pretreatment steps, is easy to use, avoids human operation errors, saves labor costs, improves work efficiency, and its mechatronics integration facilitates user control, use and maintenance of the instrument.

[0042] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A fully automated sample pretreatment device, characterized in that: Includes a weighing device for weighing the sample cup after the sample has been added; and a liquid addition device for adding buffer solution into the sample cup. An oscillating conveyor for oscillating and transferring sample cups; A conveying device for moving cuvettes; a pipetting device for extracting liquid from a shaken sample cup and injecting it into a cuvette; a cleaning device for cleaning the needle of the pipetting device. The device includes a worktable and an oscillating transmission device comprising several fixed columns. The lower ends of the fixed columns are fixedly connected to the worktable, and the upper ends of the fixed columns are connected to a fixed platform. A first drive motor is provided on the lower end face of the fixed platform, and a first coupling is provided on the fixed platform. The lower end of the first coupling is connected to the motor shaft of the first drive motor, and a first rotating rod is connected to the upper end of the first coupling. A loading tray is connected to the upper end of the first rotating rod, and several placement holes are provided on the edge of the loading tray. The weighing device includes several guide columns, the lower ends of which are fixedly connected to the worktable. Each guide column has a guide sleeve fitted onto its upper end. The upper ends of the guide sleeves are connected to a lifting platform. The lower end face of the lifting platform is connected to a threaded sleeve, and a first screw is threadedly connected to the threaded sleeve. The lower end of the first screw is connected to a second coupling. A second drive motor is disposed on the upper end face of the worktable below the lifting platform. The motor shaft of the second drive motor is connected to the second coupling. A fixed seat is disposed on the upper end face of the lifting platform. A weighing sensor is connected to the upper end of the fixed seat. A weighing pan is connected to the upper end of the weighing sensor. The weighing pan is located directly below any of the placement holes. It also includes a processor; the processor is electrically connected to the weighing device, the liquid adding device, the oscillating conveying device, the conveying device, the pipetting device, and the cleaning device; The processor also includes a liquid addition program; The liquid addition procedure includes: the processor recording the position and weight of the sample cup into which the sample is added; and the liquid addition device adding a buffer solution matching the weight of the sample cup according to the recorded position and weight of the sample cup.

2. The fully automated sample pretreatment device according to claim 1, characterized in that: The liquid dispensing device includes a base, the lower end of which is fixedly connected to the workbench. A bracket is connected to the upper end of the base, and a first sliding arm is vertically connected to the upper end of the bracket. A liquid dispensing needle is connected to the front end of the first sliding arm. The liquid dispensing needle is connected to a first peristaltic pump through a first pipeline. The first peristaltic pump is connected to a third drive motor and a buffer solution container. The liquid dispensing needle is located directly above any of the placement holes. The bracket is a hollow bracket, and pipeline holes are provided at both the upper and lower ends of the hollow bracket. The middle section of the first pipeline is located inside the hollow bracket.

3. The fully automated sample pretreatment device according to claim 1, characterized in that: The pipetting device includes two vertically fixed plates arranged opposite each other. The lower ends of the two vertically fixed plates are fixedly connected to the worktable. Vertical slide rails are provided on the opposite inner surfaces of the two vertically fixed plates. A support plate is slidably connected between the two vertical slide rails. A second screw is threadedly connected to the support plate. A fourth drive motor is provided on the upper surface of the worktable below the support plate. The motor shaft of the fourth drive motor is connected to the second screw through a third coupling. A driven wheel is rotatably connected to the upper surface of the pallet, a second rotating rod is fixedly connected to the upper end of the driven wheel, a second sliding arm is vertically connected to the upper end of the second rotating rod, and a sampling needle is connected to the front end of the second sliding arm. A fifth drive motor is fixedly connected to the lower end face of the pallet. The upper end of the motor shaft of the fifth drive motor passes through the pallet and is fixedly connected to a drive wheel. The drive wheel is connected to the driven wheel through a first synchronous belt. The sampling needle is connected to a second peristaltic pump via a second conduit, and the second peristaltic pump is connected to a sixth drive motor and a water container.

4. The fully automated sample pretreatment device according to claim 3, characterized in that: The upper ends of the two vertical fixing plates are connected to a guide plate, and the guide plate is provided with a guide hole. The second rotating rod passes through the guide hole and is slidably connected to the guide hole.

5. The fully automated sample pretreatment device according to claim 3, characterized in that: A positioning plate is fitted on the outer side of the second rotating rod, a first optocoupler is provided on the side of the positioning plate, and a second optocoupler is provided on the lower end face of the tray.

6. The fully automated sample pretreatment device according to claim 1, characterized in that: The conveying device includes a fixed frame, the lower end of which is fixedly connected to the workbench, and the upper end of which is provided with a guide rail groove. A cuvette holder is provided in the guide rail groove, and the cuvette holder is slidably connected to the guide rail groove. A movable push plate is connected to any end of the cuvette holder. The front end face of the fixed frame is provided with a guide rail, and a slider is slidably connected on the guide rail. The upper end of the slider is fixedly connected to the movable push plate. The front end face of the fixed frame is provided with a synchronous pulley at each of the left and right ends below the guide rail. A second synchronous belt is provided between the two synchronous pulleys. The upper section of the second synchronous belt is fixedly connected to the lower end of the slider. Each of the synchronous pulleys is connected to a seventh drive motor, which is installed inside the fixed frame.

7. The fully automated sample pretreatment device according to claim 1, characterized in that: The cleaning device includes a support base, the lower end of which is fixedly connected to the workbench, and the upper end of which is connected to a needle washing tank. A liquid inlet is provided in the center of the needle washing tank, and the liquid inlet is connected to a liquid inlet. The liquid inlet is connected to a third peristaltic pump through a third pipeline. The third peristaltic pump is connected to an eighth drive motor and a cleaning fluid container. The inside of the needle washing tank is provided with an annular outlet tank around the inlet tank. The outlet tank is connected to an outlet, and the outlet is connected to a waste liquid container through a fourth pipeline.

8. The fully automated sample pretreatment device according to claim 1, characterized in that: The liquid dispensing device includes a base, the lower end of which is fixedly connected to the workbench, and a bracket connected to the upper end of the base. A first sliding arm is vertically connected to the upper end of the bracket, and a liquid dispensing needle is connected to the front end of the first sliding arm. The liquid dispensing needle is connected to a first peristaltic pump through a first pipeline. The first peristaltic pump is connected to a third drive motor and a buffer solution container. The liquid dispensing needle is located directly above any of the placement holes.

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