A multi-layer resin chromatography column

Abstract

The present invention belongs to the technical field of chemical and pharmaceutical extraction equipment, and particularly relates to a multi-layer resin chromatography column, which comprises a columnar outer shell, an upper cover and a base; a conical resin column cavity with a larger upper part and a smaller lower part is arranged inside the columnar outer shell, and a plurality of horizontal arc-shaped convex strips are vertically arranged on the inner wall of the resin column cavity. A plurality of resin columns are installed in the resin column cavity. The lower end surface of the resin column is a porous sieve plate, and the upper end of the resin column has a flange, and the flange is lapped on the convex strip so that the resin column is installed in the resin column cavity; the porous sieve plate of the resin column in the upper layer is docked into the resin column in the lower layer; the outer diameter of the resin column in the lower layer is smaller than that of the resin column in the upper layer. The present invention divides the very high resin column in the prior art into multiple mutually separated resin columns that are convenient to take and replace. The resin is not prone to caking and being damaged by pressure, and it can ensure that the resin in each resin column is evenly loaded. The resin column with inactivated or damaged resin can be flexibly replaced, which is convenient and flexible.

Description

Technical Field

[0001] The present invention belongs to the technical field of chemical and pharmaceutical extraction equipment, and particularly relates to a multi-layer resin chromatography column. Background Art

[0002] The main separation and purification methods for the active ingredients of Chinese herbal medicines include macroporous resin adsorption, water extraction and alcohol precipitation, centrifugation, extraction, and membrane separation. Among them, the macroporous resin adsorption technology has great advantages due to its large adsorption capacity, good selectivity, and easy regeneration.

[0003] The liquid medicine passes through a chromatography column filled with resin for the extraction and purification of active ingredients. The aspect ratio of the resin column has a significant impact on the adsorption capacity of the resin. Generally speaking, the smaller the aspect ratio of the resin, that is, the higher the resin column, the higher the corresponding adsorption capacity of the resin. A high resin column will exert a huge pressure on the lower-layer macroporous resin, causing serious damage to the macroporous resin. Moreover, it is very difficult to load the macroporous resin evenly during column packing, resulting in poor reproducibility between product batches, affecting the product yield and the content of active ingredients. In addition, due to the relatively large pressure of the higher resin column on the lower layer, the macroporous resin is prone to caking. When some resins are inactivated, all the macroporous resins need to be dug out and replaced, causing waste of labor and resources.

[0004] Temperature is an important factor affecting adsorption and desorption. Generally speaking, low temperature is beneficial to adsorption, and high temperature is beneficial to desorption. Each Chinese herbal medicine has an optimal adsorption temperature. For example, 40°C is the optimal adsorption temperature of macroporous adsorption resin for paeoniflorin. It is very difficult for existing chromatography columns to ensure that the temperature of the resin and the liquid medicine in the chromatography column reaches the corresponding optimal temperature. In addition, in the chromatography column, the presence of air bubbles in the resin column will affect the adsorption effect of the macroporous resin.

[0005] The liquid medicine flows into the chromatography column from the liquid inlet on the upper cover of the chromatography column. Since the liquid medicine flows into the column body, it will impact the resin at a certain height from the liquid inlet, easily causing splashing, and at the same time damaging the shape of the resin column. On the other hand, the liquid only flows to a certain position of the resin column, so that each position of the resin column cannot be evenly and fully utilized. Summary of the Invention

[0006] The object of the present invention is to overcome the defects of uneven resin packing and easy caking of resin in the prior art, and to provide a multi-layer resin chromatography column that is convenient for evenly packing resin and has good adsorption effect.

[0007] The technical solution adopted by the present invention to solve its technical problems is:

[0008] A multi-layer resin chromatography column, characterized in that: it includes a columnar outer shell, an upper cover covering the columnar outer shell, and a base joined to the bottom end of the columnar outer shell; a conical resin column cavity with a larger upper part and a smaller lower part is arranged inside the columnar outer shell, and a plurality of horizontal arc-shaped ridges are vertically arranged on the inner wall of the resin column cavity. A plurality of resin columns are installed in the resin column cavity. The resin column is a cylindrical structure with an open upper end. The lower end surface of the resin column is a porous sieve plate. The upper end of the resin column has a flange, and the flange is lapped on the ridge to install the resin column in the resin column cavity; the porous sieve plate of the resin column in the upper layer is docked into the resin column in the lower layer; the outer diameter of the resin column in the lower layer is smaller than the outer diameter of the resin column in the upper layer.

[0009] Further, a heating installation cavity is also arranged outside the resin column cavity in the columnar outer shell, and an infrared heating tube is installed in the heating installation cavity.

[0010] Further, the outer peripheral surface of the resin column is conical with a larger upper part and a smaller lower part.

[0011] Further, a semi-circular handle is rotatably connected to the upper end of the resin column, and the handle fits on the upper surface of the flange after being laid down.

[0012] Further, a temperature sensor is installed on the inner wall of the resin column cavity.

[0013] Further, a flow equalizing and guiding member is arranged at the lower part of the upper cover. The flow equalizing and guiding member includes a flow equalizing plate, a flow limiting bar arranged at the upper edge of the flow equalizing plate, and a conical guiding plate arranged at the lower side of the flow equalizing plate. The flow equalizing plate has a plurality of flow equalizing holes.

[0014] Further, a flow equalizing and splash-proof plate is arranged below the liquid inlet in the upper cover, and the flow equalizing and splash-proof plate has a plurality of flow equalizing holes.

[0015] Further, an ultrasonic transmitter is installed on the upper cover or the base.

[0016] Furthermore, resin is loaded into the resin column, and the resin loaded in the lower resin column does not contact the porous sieve plate of the upper resin column.

[0017] Compared with the prior art, the beneficial effects of a multi-layer resin chromatography column of the present invention are:

[0018] 1. By setting the resin column cavity in a conical shape with a larger upper part and a smaller lower part, the resin column, which is very tall in the prior art, is divided into multiple mutually separated resin columns that are convenient to pick up and replace. The resin is not likely to form lumps or be damaged by pressure. After each layer of resin column is filled with resin, multiple layers of resin columns are placed from bottom to top, which can ensure uniform filling of the resin in each layer of resin column. And if the resin in a certain layer of resin column becomes inactivated or damaged, only this layer of resin column needs to be replaced separately, which is convenient and flexible.

[0019] 2. The resin column is heated by an infrared heating tube to ensure the optimal temperature during adsorption and meet the requirements of high-temperature desorption.

[0020] 3. The liquid medicine is made to flow evenly through a uniform-flow splash-proof plate and a uniform-flow guiding member, so that the liquid medicine is evenly sprayed on the resin column of the top layer without causing splashing.

[0021] 4. By emitting ultrasonic waves through an ultrasonic transmitter, the vibration of air bubbles in the resin can be effectively caused, so that the air bubbles burst, achieving the purpose of defoaming and improving the adsorption capacity of the resin. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The present invention will be further described in detail below with reference to the drawings and specific embodiments.

[0023] Figure 1 It is the overall structure diagram of the embodiment of the present invention;

[0024] Figure 2 is Figure 1 the enlarged view of part A in

[0025] Figure 3 It is the three-dimensional view of the columnar housing;

[0026] Figure 4 It is the top view of the assembly of the columnar housing and the resin column;

[0027] Figure 5 It is the three-dimensional view of the resin column;

[0028] Figure 6 It is the upper-view three-dimensional view of the uniform-flow guiding member;

[0029] Figure 7 It is the lower-view three-dimensional view of the uniform-flow guiding member.

[0030] In the figure: 1. Base, 2. Upper cover, 3. Columnar housing, 31. Resin column cavity, 32. Rib, 33. Heating installation cavity, 4. Resin column, 41. Porous sieve plate, 42. Flange, 43. Handle, 5. Infrared heating tube, 6. Uniform-flow guiding member, 61. Uniform-flow plate, 62. Current-limiting bar, 64. Conical guiding plate, 7. Uniform-flow splash-proof plate, 8. Ultrasonic transmitter, 9. Uniform-flow hole. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0032] like Figures 1 - 7 The specific embodiment of a multi-layer resin chromatography column of the present invention shown in the figure comprises a columnar shell 3, an upper cover 2 covering the columnar shell 3 and a base 1 connected to the bottom end of the columnar shell 3; a conical resin column cavity 31 with a larger top and a smaller bottom is arranged in the columnar shell 3, and a plurality of horizontal arc convex strips 32 are vertically arranged on the inner wall of the resin column cavity 31, and a plurality of resin columns 4 are installed in the resin column cavity 31, the resin column 4 is a cylindrical structure with an open upper end, and the lower end surface of the resin column 4 is a porous sieve plate 41, and the upper end of the resin column 4 has a flange 42, and the flange 42 overlaps the convex strip 32 so that the resin column 4 is installed in the resin column cavity 31; in order to make the porous sieve plate 41 of the resin column 4 of the upper layer docked into the resin column 4 of the lower layer, the outer peripheral surface of the resin column 4 is a conical shape with a larger top and a smaller bottom, and the outer diameter of the resin column 4 of the lower layer is smaller than the outer diameter of the resin column 4 of the upper layer. A semicircular handle 43 is rotatably connected to the upper end of the resin column 4 , and the handle 43 is laid down to fit on the upper surface of the flange 42 .

[0033] When loading the resin into the resin column 4, a breathable and water-permeable sponge paper can be placed on the porous sieve plate 41, and the diameter-to-height ratio of each layer of the resin column 4 can be set to 1:1-1:2. Since the height of each layer of the resin column 4 is relatively low, it is easy to load the resin evenly. In order to prevent the porous sieve plate 41 of the upper resin column 4 from being pressed by the porous sieve plate 41 of the upper resin column 4 after it is docked into the lower resin column 4, do not fill the resin too full when loading the resin, and ensure that the loaded resin in the lower resin column 4 does not contact the porous sieve plate 41 of the upper resin column 4.

[0034] Since the optimal adsorption temperature of each Chinese herbal medicine is different, traditional liquid medicine adsorption generally does not consider the influence of temperature, or at most controls the temperature of the liquid medicine entering the chromatography column. However, after the temperature of the liquid medicine is exchanged with the resin in the chromatography column, it is difficult to reach the optimal temperature. In order to make the resin have the best adsorption effect, a heating device is added in this embodiment. A heating installation cavity 33 is provided outside the resin column cavity 31 in the columnar housing 3, an infrared heating tube 5 is installed in the heating installation cavity 33, and a temperature sensor is installed on the inner wall of the resin column cavity 31. The infrared heating tube 5 can heat the resin and liquid medicine at any time, and through the monitoring of the temperature sensor, it is ensured that the temperature is in the optimal adsorption state of the resin during the whole process of resin adsorption.

[0035] In order to prevent the liquid medicine from flowing to the same position of the resin column 4, a uniform flow guide 6 is provided at the lower part of the upper cover 2, see Figure 6 and Figure 7, the uniform flow guiding member 6 includes a uniform flow plate 61, a current limiting bar 62 provided on the upper edge of the uniform flow plate 61, and a conical guiding plate 64 provided on the lower side of the uniform flow plate 61. The uniform flow plate 61 is provided with a plurality of uniform flow holes 9.

[0036] See Figure 2 , in order to further uniform the flow and prevent the splashing of the liquid medicine, a uniform flow and splash-proof plate 7 is provided inside the upper cover 2 near the lower part of the liquid inlet. The uniform flow and splash-proof plate 7 is provided with a plurality of uniform flow holes 9.

[0037] The bubbles in the resin will affect the adsorption effect of the resin. Since it is inconvenient to defoam the chromatography resin by mechanical stirring, and introducing a defoaming agent will affect the extraction of drug components. Generally, in production, the influence of the bubbles in the resin can only be ignored. In this embodiment, by installing an ultrasonic transmitter 8 on the upper cover 2 or the base 1, the ultrasonic wave is used to vibrate and break the bubbles to achieve the defoaming effect.

[0038] The specific operation steps of the multi-layer resin chromatography column of this embodiment are as follows:

[0039] Resins are respectively filled into a plurality of resin columns 4. The resins should not be filled too full. Then, according to the outer diameter of the outer peripheral surface of the resin column 4 from small to large, the appropriate resin columns 4 are sequentially placed from bottom to top. Hold the handle and place the resin column 4 on the corresponding flange 42. After placing it in place, release the handle, and the handle will automatically fall down and fit on the upper surface of the flange 42. Turn on the infrared heating tube 5, and first heat the temperature inside the chromatography column to the optimal adsorption temperature of the traditional Chinese medicine to be extracted. When the liquid medicine enters the chromatography column from the upper cover 2, it is uniformly flowed through the uniform flow and splash-proof plate 7, further uniformly flowed through the uniform flow guiding member 6, and then introduced onto the uppermost resin column 4. The liquid medicine is uniformly sprayed on the uppermost resin column 4.

[0040] In the present invention, the resin column cavity 31 is set to be a conical shape with a larger upper part and a smaller lower part. The existing high resin column 4 is divided into a plurality of mutually separated resin columns 4 that are convenient to take and replace. The resin is not prone to caking and being crushed and damaged. After each layer of resin column 4 is filled with resin, multiple layers of resin columns 4 are placed from bottom to top, which can ensure that the resin in each layer of resin column 4 is uniformly filled. And if the resin in a certain layer of resin column 4 is inactivated or damaged, only this layer of resin column 4 needs to be replaced separately, which is convenient and flexible.

[0041] It should be understood that the specific embodiments described above are only used to explain the present invention and are not used to limit the present invention. The obvious changes or variations derived from the spirit of the present invention are still within the protection scope of the present invention.

Claims

1. A multi-layer resin chromatography column, characterized in that: It includes a columnar outer shell (3), an upper cover (2) covering the columnar outer shell (3), and a base (1) joined to the bottom end of the columnar outer shell (3); a conical resin column cavity (31) with a larger upper part and a smaller lower part is arranged inside the columnar outer shell (3), and a plurality of horizontal arc-shaped ridges (32) are vertically arranged on the inner wall of the resin column cavity (31). A plurality of resin columns (4) are installed in the resin column cavity (31). The resin column (4) is a cylindrical structure with an open upper end. The lower end surface of the resin column (4) is a porous sieve plate (41). The upper end of the resin column (4) has a flange (42). The flange (42) is lapped on the ridge (32) to install the resin column (4) in the resin column cavity (31). The porous sieve plate (41) of the resin column (4) in the upper layer is docked into the resin column (4) in the lower layer. The outer diameter of the resin column (4) in the lower layer is smaller than that of the resin column (4) in the upper layer. Resin is loaded into the resin column (4), and the resin loaded in the lower resin column (4) does not contact the porous sieve plate (41) of the upper resin column (4). The outer peripheral surface of the resin column (4) is conical with a larger upper part and a smaller lower part. A semi-circular handle (43) is also rotatably connected to the upper end of the resin column (4). After the handle (43) is laid down, it fits on the upper surface of the flange (42).

2. The multi-layer resin chromatography column according to claim 1, wherein: An infrared heating tube (5) is installed in a heating installation cavity (33) arranged outside the resin column cavity (31) inside the columnar outer shell (3).

3. A multi-layer resin chromatography column according to claim 1, characterized in that: A temperature sensor is installed on the inner wall of the resin column cavity (31).

4. A multi-layer resin chromatography column according to claim 1, characterized in that: A flow equalizing and guiding member (6) is arranged at the lower part of the upper cover (2). The flow equalizing and guiding member (6) includes a flow equalizing plate (61), a current limiting blocking strip (62) arranged at the upper edge of the flow equalizing plate (61), and a conical guiding plate (64) arranged at the lower side of the flow equalizing plate (61). The flow equalizing plate (61) has a plurality of flow equalizing holes (9).

5. The multi-layer resin chromatography column according to claim 4, wherein: A flow equalizing and splash-proof plate (7) is arranged inside the upper cover (2) near the lower side of the liquid inlet. The flow equalizing and splash-proof plate (7) has a plurality of flow equalizing holes (9).

6. The multi-layer resin chromatography column according to claim 1, wherein: An ultrasonic transmitter (8) is installed on the upper cover (2) or the base (1).

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