PCR instrument capable of automatically adjusting heating range

Abstract

The present application relates to a kind of PCR instrument of automatically adjustable heating range, including main body, heating device and test solution pipe, the main body is equipped with top cover, the heating device includes heating module and temperature control module, the top cover is located in the heating device, the heating device further includes main heating part and vice heating part, the heating module and the temperature control module are located in the main heating part, the vice heating part is equipped with several heating grooves, the side of the heating groove facing the top cover is equipped with test solution port adapted to the test solution pipe, the position corresponding the test solution port of the heating groove facing the main heating part is equipped with elastic switch, the distance between the elastic switch and the test solution port is less than the length of the test solution pipe.The present application provides a kind of PCR instrument that can automatically and uniformly heat the position of test solution.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological detection, in particular to a PCR instrument capable of automatically adjusting heating range. BACKGROUND

[0002] Polymerase chain reaction (PCR) is a molecular biology technique for amplifying specific DNA fragments, which can be regarded as a special DNA replication in vitro. The biggest feature of PCR is that it can greatly increase the amount of DNA. PCR uses the principle that DNA denatures into single strands at high temperature in vitro, and primers bind to single strands according to the principle of base complementary pairing at low temperature. Then the temperature is adjusted to the optimum reaction temperature of DNA polymerase, and the DNA polymerase synthesizes complementary chains along the phosphate to pentose (5'-3') direction. The PCR instrument based on polymerase is actually a temperature control device that can well control the denaturation temperature, annealing temperature and extension temperature.

[0003] However, the existing PCR instrument usually uses a heating unit on the top cover to heat the test solution from above. This heating method will heat all test solution positions, regardless of whether there is test solution at the corresponding position. This method wastes power and the temperature of the test solution will slowly conduct from top to bottom. When the temperature of the upper layer reaches the given temperature, the temperature of the lower layer of the test solution often needs a period of time to reach the given temperature, which is not good. The top cover may also be deformed by heat, bulging in the middle, and the test solution tube may not fit completely, so the test solution may evaporate and escape from the gap between the two, affecting the accuracy of the PCR detection result. SUMMARY

[0004] In summary, in order to overcome the shortcomings of the prior art, the present application provides a PCR instrument capable of automatically and uniformly heating the positions where the test solution is placed.

[0005] To achieve the above purpose, the present application provides the following technical scheme: a PCR instrument capable of automatically adjusting heating range, comprising a main body, a heating device and a test solution tube, the main body is provided with a top cover, the heating device comprises a heating module and a temperature control module, the top cover is located above the heating device, the heating device further comprises a main heating part and a secondary heating part, the heating module and the temperature control module are located in the main heating part, the secondary heating part is provided with a plurality of heating grooves, one side of the heating groove facing the top cover is provided with a test solution port matched with the test solution tube, the position of the heating groove facing the main heating part corresponding to the test solution port is provided with an elastic switch, and the distance between the elastic switch and the test solution port is less than the length of the test solution tube.

[0006] In this way, the test solution tube containing the test solution is placed in the test solution port according to the number of tests required, and the test solution tube is in contact with the heat-conducting liquid in the heating groove. At this time, the test solution tube will stay on the elastic switch due to gravity, and then the cover is closed, so that the test solution tube is pressed down by the cover, the elastic switch is pressed open, the heat-conducting liquid in the heating groove in the secondary heating part is in communication with the heat-conducting liquid in the primary heating part, and the elastic switch of the heating groove without the test solution tube is not pressed open, so that the heat-conducting liquid in the heating groove is isolated from the heat-conducting liquid in the primary heating part. At this time, the heating module will conduct heat by the heat-conducting liquid in the primary heating part, so that the heat-conducting liquid in the heating groove with the test solution tube is heated to the temperature set by the temperature control module, and the heating groove without the test solution tube will not be heated, thereby concentrating the heating range and reducing the waste of electricity. The heat-conducting liquid uniformly adheres to the surface of the test solution tube, and the test solution tube is heated from all directions, so that the heating is uniform, the cover will not be deformed by heat, and the test solution will not be vaporized and discharged, thereby affecting the accuracy of the PCR detection result.

[0007] Further, the elastic switch is an elastic plate, and the end of the elastic switch is located below the test solution port.

[0008] In this way, when the test solution tube is pressed down by the cover, the end of the elastic plate is pressed, so that the elastic plate is bent. The bent elastic plate is no longer in the same plane as the bottom surface of the heating groove, and the heat-conducting liquid in the heating groove is in communication with the primary heating part through the bent part of the elastic plate.

[0009] Further, the edge of the elastic switch is provided with a rubber ring.

[0010] In this way, when the elastic plate is not bent, the rubber ring is attached to the bottom of the heating groove, further isolating the heating groove from the primary heating part, and preventing the heating module from heating the heating groove without the test solution tube.

[0011] Further, the side of the test solution port facing the elastic switch is provided with a plurality of positioning blocks, and the positioning blocks are uniformly distributed on the test solution port.

[0012] In this way, when the elastic plate is bent by the test solution tube, the positioning blocks uniformly distributed on the test solution port can fix the test solution tube to keep it vertical and not be tilted by the elastic plate. The heat-conducting liquid can heat the test solution tube from the space between the positioning blocks.

[0013] Further, the side of the test solution port facing the elastic switch is provided with a sealing bag matched with the test solution tube.

[0014] In this way, the heat-conducting liquid is sealed in the heating groove, and even if the instrument is tilted, the heat-conducting liquid will not flow out of the heating groove. After the test solution tube is inserted, the sealing band will tightly adhere to the test solution tube, and will not affect the heating effect.

[0015] Further, the heating device is provided with a cooling tank on each side, the cooling tank and the main heating part are respectively provided with a water pump on the side facing each other, the cooling tank is provided with a control mechanism, and the control mechanism controls the opening and closing of the water pump.

[0016] In this way, when cooling is needed, the control mechanism controls the water pump in the empty cooling tank to pump out the heat-conducting liquid in the main heating part, and the water pump on the other side of the main heating part pumps the heat-conducting liquid in the cooling tank filled with normal-temperature heat-conducting liquid into the main heating part, directly replacing the heat-conducting liquid, without waiting for natural cooling or slowly cooling by using a cooling device, so that the cooling speed is faster, and when the cooling tank on one side is pumped out, the control mechanism controls the cooling tank on the other side to be pumped out in the next operation, so that the two sides are repeatedly pumped out, without manual replacement.

[0017] Further, the control mechanism is an infrared module, and the control mechanism is located on the lower half of the side of the cooling tank.

[0018] In this way, when the cooling tank contains more than half of the heat-conducting liquid, the infrared module on the lower half of the side of the cooling tank detects the presence of the heat-conducting liquid, and at this time, the water pump in the other cooling tank is controlled to pump water, and when the water level of the heat-conducting liquid is pumped below the infrared module, the infrared module cannot detect the heat-conducting liquid, and at this time, the infrared module on the other side is controlled to stop operating after operating, and the infrared module on the other side controls the water pump on this side to pump water, so that the two sides are repeatedly pumped out.

[0019] Further, the cooling tank is provided with a refrigeration module, and the control mechanism controls the opening and closing of the refrigeration module.

[0020] In this way, the heat-conducting liquid pumped out of the main heating part from the cooling tank is low-temperature heat-conducting liquid, and the instrument can control the temperature of the test liquid to be below normal temperature, and can also quickly cool the temperature above normal temperature to the required temperature by the heating module.

[0021] Further, the instrument further comprises a storage battery.

[0022] In this way, the instrument can be operated by relying on the storage battery, and the detection can be completed even if there is no power socket for the accessories.

[0023] Further, the storage battery is provided with a thermoelectric power generation sheet.

[0024] In this way, the temperature difference generated when the instrument is operated will generate electricity and be stored in the storage battery, and the storage battery does not need to be deliberately charged, which is more convenient. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 The drawings show the structure of the embodiment of the application.

[0026] Figure 2A-A sectional view of the heating device of the embodiment of the present application.

[0027] Figure 3 B-B sectional view of the heating device of the embodiment of the present application. Figure 2

[0028] Figure 4 B-B sectional view of the heating device of the embodiment of the present application. Figure 3

[0029] Figure 5 B-B sectional view of the heating device of the embodiment of the present application. Figure 4

[0030] Figure 6 B-B sectional view of the heating device of the embodiment of the present application. Figure 4

[0031] Figure 7 B-B sectional view of the heating device of the embodiment of the present application. Figure 3

[0032] Figure 8 B-B sectional view of the heating device of the embodiment of the present application. Figure 3

[0033] Figure 9 B-B sectional view of the heating device of the embodiment of the present application. Figure 8

[0034] B-B sectional view of the heating device of the embodiment of the present application. DETAILED DESCRIPTION

[0035] The specific embodiments are only an explanation of the embodiments, which are not a limitation of the embodiments, and the person skilled in the art can make a modification of the embodiments without a creative contribution according to the needs after reading the present specification, but as long as the modification is within the scope of the claims of the embodiments, it is protected by the patent law.

[0036] ​​​​​​​The application provides the following technical scheme: a PCR instrument capable of automatically adjusting a heating range, comprising a main body 1, a heating device 2 and a test liquid pipe 3, wherein the main body 1 is provided with a top cover 101, the heating device 2 comprises a heating module 201 and a temperature control module 202, the top cover 101 is located above the heating device 2, the heating device 2 further comprises a main heating part 203 and a sub heating part 204, the heating module 201 and the temperature control module 202 are located in the main heating part 203, the sub heating part 204 is provided with a plurality of heating grooves 2041, one side of the heating grooves 2041 facing the top cover 101 is provided with a test liquid port 20411 matched with the test liquid pipe 3, and the position of the test liquid port 20411 corresponding to the main heating part 203 of the sub heating part 204 is provided with an elastic switch 20412, and the distance between the elastic switch 20412 and the test liquid port 20411 is less than the length of the test liquid pipe 3.

[0037] In this way, the test liquid pipe 3 containing test liquid is placed in the test liquid port 20411 according to the number of required detections, the test liquid pipe 3 is in contact with the heat-conducting liquid in the heating groove 2041, at this time, the test liquid pipe 3 stays on the elastic switch 20412 due to gravity, then the top cover 101 is covered, the test liquid pipe 3 is pressed down by the top cover 101, the elastic switch 20412 is pressed open, the heat-conducting liquid in the heating groove 2041 of the sub heating part 204 is in communication with the heat-conducting liquid of the main heating part 203, and the heating groove 2041 without the test liquid pipe 3 is not pressed open by the elastic switch 20412 when the top cover 101 is covered, so that the heat-conducting liquid in the heating groove 2041 is in an isolated state from the heat-conducting liquid in the main heating part 203, at this time, the heating module 201 conducts heat by the heat-conducting liquid of the main heating part 203, so that the heat-conducting liquid in the heating groove 2041 containing the test liquid pipe 3 is heated to the temperature set by the temperature control module 202, and the heating groove 2041 without the test liquid pipe 3 is not heated, thereby the heating range is concentrated and the waste of electricity is reduced, the heat-conducting liquid uniformly adheres to the surface of the test liquid pipe 3, the test liquid pipe 3 is heated from all directions, the heating is uniform, and the top cover 101 is not deformed by heat, so that the test liquid is not dispersed as steam, and the accuracy of the PCR detection result is affected.

[0038] Preferably, the elastic switch 20412 is an elastic plate, and the end of the elastic switch 20412 is located below the test liquid port 20411.

[0039] In this way, when the test liquid pipe 3 is pressed down by the top cover 101, the end of the elastic plate is pressed, so that the elastic plate is bent, the bent elastic plate is no longer in the same plane as the bottom surface of the heating groove 2041, and the heat-conducting liquid in the heating groove 2041 is in communication with the main heating part 203 from the bent part of the elastic plate.

[0040] Preferably, the edge of the elastic switch 20412 is provided with a rubber ring 204121.

[0041] In this way, the rubber ring 204121 is in contact with the bottom of the heating groove 2041 when the elastic plate is not bent, further isolating the heating groove 2041 from the main heating part 203, and avoiding the heating module 201 from heating the heating groove 2041 without the test solution tube 3.

[0042] Preferably in this embodiment, the side of the test solution port 20411 facing the elastic switch 20412 is provided with a plurality of positioning blocks 204111, which are evenly distributed on the test solution port 20411.

[0043] In this way, the test solution tube 3 will be fixed by the positioning blocks 204111 evenly distributed on the test solution port 20411 when the elastic plate is bent, so that the test solution tube 3 remains vertical and is not tilted by the elastic plate, and the heat-conducting liquid can heat the test solution tube 3 from the space between the positioning blocks 204111.

[0044] Preferably in this embodiment, the side of the test solution port 20411 facing the elastic switch 20412 is provided with a sealing bag 204112 adapted to the test solution tube 3.

[0045] In this way, the heat-conducting liquid is sealed in the heating groove 2041, so that even if the instrument is tilted, the heat-conducting liquid will not flow out of the heating groove 2041, and the sealing band will be in close contact with the test solution tube 3 after insertion, without affecting the heating effect.

[0046] Preferably in this embodiment, the heating device 2 is provided with a cooling tank 205 on each side, the cooling tank 205 and the main heating part 203 are respectively provided with a water pump 2051 on the side facing each other, and the cooling tank 205 is provided with a control mechanism 2052 that controls the opening and closing of the water pump 2051.

[0047] In this way, when cooling is needed, the control mechanism 2052 controls the water pump 2051 in the empty cooling tank 205 to pump out the heat-conducting liquid in the main heating part 203, and the water pump 2051 on the other side of the main heating part 203 pumps the heat-conducting liquid in the cooling tank 205 filled with normal-temperature heat-conducting liquid, directly replacing the heat-conducting liquid without waiting for natural cooling or using cooling equipment to slowly cool it down, so that the cooling speed is faster, and when the cooling tank 205 on one side is full, the control mechanism 2052 controls the cooling tank 205 on the other side to be pumped out next time, so that the process is repeated without manual replacement.

[0048] Preferably in this embodiment, the control mechanism 2052 is an infrared module, and the control mechanism 2052 is located on the lower half of the side of the cooling tank 205.

[0049] In this way, when the cooling box 205 contains more than half of the heat-conducting liquid, the infrared module on the lower half of the side of the cooling box 205 can detect the presence of the heat-conducting liquid. At this time, the water pump 2051 in the other cooling box 205 is controlled to suck water. When the water level of the heat-conducting liquid is lowered to below the infrared module, the infrared module cannot detect the heat-conducting liquid. At this time, the infrared module on the other side is controlled to stop running after running, and the infrared module on the other side controls the water pump 2051 on this side to suck water. This process is repeated.

[0050] Preferably, the cooling box 205 is provided with a refrigeration module 2053, and the control mechanism 2052 controls the opening and closing of the refrigeration module 2053.

[0051] In this way, the heat-conducting liquid extracted from the cooling box 205 by the main heating part is low-temperature heat-conducting liquid. The instrument can control the test liquid to be below room temperature, and can also quickly reduce the temperature above room temperature, and then adjust the temperature to the required temperature by the heating module 201.

[0052] Preferably, the embodiment further comprises a storage battery 4.

[0053] In this way, the instrument can be operated by relying on the storage battery 4, and even if the accessory does not have a power socket, the detection can be completed.

[0054] Preferably, the storage battery 4 is provided with a thermoelectric power generation sheet 401.

[0055] In this way, the temperature difference generated when the instrument is running will generate electricity and be stored in the storage battery 4. There is no need to deliberately charge the storage battery 4, which is more convenient.

Claims

1. A PCR instrument with automatically adjustable heating range, comprising a main body, a heating device, and test solution tubes, wherein the main body is provided with a top cover, the heating device includes a heating module and a temperature control module, and the top cover is located above the heating device, characterized in that: The heating device further includes a main heating section and a secondary heating section. The heating module and the temperature control module are located in the main heating section. The secondary heating section is provided with several heating tanks. The side of each heating tank facing the top cover has a test liquid port adapted to the test liquid tube. The heating tank facing the main heating section has a flexible switch at the position corresponding to the test liquid port. The distance between the flexible switch and the test liquid port is less than the length of the test liquid tube. The flexible switch is a flexible plate, and the end of the flexible switch is located below the test liquid port. The side of the test liquid port facing the flexible switch has a sealing bag adapted to the test liquid tube. The heating tank contains a heat-conducting liquid.

2. The PCR instrument with automatically adjustable heating range according to claim 1, characterized in that: The elastic switch has a rubber ring at its edge.

3. A PCR instrument with automatically adjustable heating range according to claim 1, characterized in that: A plurality of positioning blocks are provided on the side of the test liquid port facing the elastic switch, and the positioning blocks are evenly distributed on the test liquid port.

4. A PCR instrument with automatically adjustable heating range according to claim 1, characterized in that: The heating device is provided with cooling boxes on both sides. The cooling boxes and the main heating part are respectively provided with water pumps on the opposite side. The cooling boxes are provided with a control mechanism, which controls the opening and closing of the water pumps.

5. A PCR instrument with automatically adjustable heating range according to claim 4, characterized in that: The control mechanism is an infrared module, and it is located on the lower half of the side of the cooling box.

6. A PCR instrument with automatically adjustable heating range according to claim 4, characterized in that: The cooling box is equipped with a refrigeration module, and the control mechanism controls the opening and closing of the refrigeration module.

7. A PCR instrument with automatically adjustable heating range according to claim 1, characterized in that: It also includes storage batteries.

8. A PCR instrument with automatically adjustable heating range according to claim 7, characterized in that: The battery is equipped with thermoelectric generators.

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