A sedimentation bottle

Abstract

The present invention relates to a pleural effusion sedimentation bottle, comprising a storage part for accommodating pleural effusion, the storage part is provided with an internal cavity for accommodating pleural effusion, the upper part of the storage part is provided with a liquid inlet for loading pleural effusion and a sealing member for detachably closing the liquid inlet, the bottom of the storage part is provided with a sample discharge port for discharging target cells and a control member for controlling the opening / closing of the sample discharge port, the side of the lower part of the storage part is provided with a liquid discharge port for discharging supernatant and a regulating member for controlling the opening / closing of the liquid discharge port, the liquid inlet, the sample discharge port and the liquid discharge port are respectively connected with the internal cavity; the sedimentation bottle has a simple and compact structure, is not only convenient for collecting, storing and transferring pleural effusion, but also can conveniently and safely handle the supernatant while fully retaining the cell suspension and / or sediment, thereby avoiding the biosafety problem caused by arbitrary discharge.

Description

Technical Field

[0001] The invention relates to the technical field of medical devices, and in particular to a pleural and abdominal effusion precipitation bottle. Background Art

[0002] Pleural effusion examination is used clinically to examine the nature of ascites in the chest, so as to determine the cause and etiology of ascites formation, and play a guiding role in the treatment of clinical diseases. There are many causes, such as cardiovascular disease, liver disease, peritoneal disease, kidney disease, nutritional disorders, malignant tumors, peritoneal metastasis, ovarian tumors, connective tissue diseases, etc. Diseases form excessive pleural effusions, which are called pleural effusions and ascites. The effusions can be divided into transudates and exudates according to their nature.

[0003] In clinical practice, it is usually necessary to collect the patient's pleural effusion and ascites and send them for cytopathological examination. However, the existing technology lacks containers specifically used for collecting and storing pleural effusion, resulting in a variety of existing pleural effusion collection containers. Some use used infusion glass bottles to collect pleural effusion, some use mineral water bottles to collect pleural effusion, and some use containers such as cups to collect pleural effusion. In addition to tumor cells, pleural effusion may also contain a variety of pathogens, viruses and microorganisms. Using such containers to collect pleural effusion not only poses a biosafety hazard, but also the containers cannot be sterile, and the cells sent for examination are prone to degeneration, affecting subsequent detection and diagnosis. After the specimen arrives at the pathology department, it needs to be left to stand and the precipitate (i.e., target cells) is taken for examination. Cytological testing, while using existing containers to collect pleural and abdominal effusions, when taking the sediment in the container, it is necessary to repeatedly pour out the supernatant in the container. In this process, the sediment that has been precipitated (i.e., target cells) will be lifted up while pouring, mixed with the supernatant, and finally poured out, resulting in the loss of important cell components, which seriously affects subsequent testing and diagnosis; in addition, in the container in the prior art, the supernatant separated from the collected pleural and abdominal effusions is usually directly poured out. Since the supernatant must contain components such as the patient's cells, there must be a biosafety problem in treating the supernatant in this way, and the prior art does not provide any effective technical means to solve this problem. Summary of the invention

[0004] The purpose of the present invention is to improve the deficiencies in the prior art and to provide a sedimentation bottle with a simple and compact structure, which is specially used for collecting, storing and separating pleural effusion and ascites. It is not only convenient for collecting cell suspension and / or sediment, but also can conveniently and safely handle supernatant and avoid biosafety problems caused by arbitrary discharge.

[0005] The technical solution adopted by the present invention to solve its technical problem is:

[0006] A pleural effusion sedimentation bottle comprises a storage part for accommodating pleural effusion, the storage part is provided with an internal cavity for accommodating pleural effusion, the upper part of the storage part is provided with a liquid inlet for loading pleural effusion and a sealing member for detachably closing the liquid inlet, the bottom of the storage part is provided with a sample discharge port for discharging target cells and a control member for controlling the opening / closing of the sample discharge port, the side of the lower part of the storage part is provided with a liquid discharge port for discharging supernatant and a regulating member for controlling the on / off of the liquid discharge port, and the liquid inlet, the sample discharge port and the liquid discharge port are respectively communicated with the internal cavity. The present invention provides a sedimentation bottle specially used for collecting and storing pleural and ascites fluid. When in use, the collected pleural and ascites fluid can be added into the internal cavity of the storage part through the liquid inlet and sealed with a sealing member, which is convenient for preserving the pleural and ascites fluid and preventing the pleural and ascites fluid from being contaminated. When the sedimentation bottle is sent to the pathology department, the cell suspension and / or sediment (i.e., target cells) in the pleural and ascites fluid can be automatically deposited to the bottom of the sedimentation bottle by letting the sedimentation bottle stand still. After the prescribed time has passed, the regulating part can be opened to conveniently discharge the supernatant from the liquid discharge port without tilting or flipping the sedimentation bottle, and the supernatant can be discharged from the liquid discharge port. The cell suspension and / or sediment is retained at the bottom of the internal cavity and will not be discharged as the sedimentation bottle is tilted or turned over; finally, the liquid inlet can be conveniently opened through the control part, so that the cell suspension and / or sediment can be conveniently taken out from the sedimentation bottle for cytological detection; it can be seen that the use of the sedimentation bottle is not only convenient for the collection, storage and transfer of pleural and abdominal fluids, but also can fully retain the cell suspension and / or sediment while conveniently removing the upper cleaning solution, so that the upper cleaning solution is separated from the target cells, so that the cell suspension and / or sediment can be conveniently collected for cytological detection.

[0007] In one embodiment, the storage part includes a bottle body, the liquid inlet is arranged at the upper part of the bottle body, the lower end of the bottle body is provided with a necking part with a gradually decreasing inner diameter, the sample discharge port is arranged at the lower end of the necking part, the outer side of the necking part is provided with a base for supporting the bottle body, and the liquid discharge port is arranged at the bottle body or the necking part. The bottle body can be stably placed on the table by providing the base, and the necking part can be provided to facilitate the enrichment of the cell suspension and / or sediment in the necking part, and finally discharged from the necking part to the present precipitation bottle.

[0008] In one solution, the base is a cylindrical structure, such as a round cylinder or a conical cylinder structure.

[0009] For the convenience of operation, in another solution, the base includes at least three supporting pieces, which are respectively distributed along the circumference of the bottle body, and the upper ends of the supporting pieces are respectively connected to the bottle body or the necked portion, and the supporting pieces are used to support the bottle body. This not only facilitates the bottle body to be stably placed on the table, but also facilitates the user to operate the control element through the gap between the two connected supporting pieces, which is conducive to more convenient use.

[0010] In order to facilitate the smooth removal of large-volume sediments from the bottle body, the necking part is further detachably fixed to the lower end of the bottle body, and the liquid discharge port is arranged at the necking part. The necking part and the bottle body are connected in a detachable manner, which facilitates the separation of the necking part and the bottle body, and not only can the bottle body be separated separately, but also large-volume sediments can be conveniently removed from the upper end of the necking part.

[0011] Preferably, the regulating part is a valve.

[0012] To achieve detachable connection, preferably, the lower end of the bottle body is provided with an external thread, and the upper end of the necked portion is provided with an internal thread matched with the external thread. The detachable connection is achieved through the cooperation of the external thread and the internal thread.

[0013] Preferably, the sealing member is a rubber stopper, or the sealing member is a sealing cover, the sealing cover is provided with a threaded hole, the threaded hole is provided with an internal thread, and the liquid inlet is provided with an external thread matching the internal thread. It can achieve both sealing and detachable connection, which is more convenient to use.

[0014] To facilitate the discharge of the cell suspension and / or sediment at the bottom of the internal cavity, the control member further comprises a bottom cover, which is detachably fixed to the sample discharge port. When in use, the user can open the sample discharge port by opening the bottom cover to discharge the cell suspension and / or sediment.

[0015] For the case where only the sediment is needed, the bottom cover is further provided with a communication port, the communication port is provided with a communication pipe, and the communication pipe is provided with a valve. When the sediment at the bottom of the sedimentation bottle needs to be taken out, the valve can be opened first, the liquid around the sediment can be discharged through the communication port, and then the valve can be closed and the bottom cover can be opened, so that the sediment with less water content can be obtained, which is more convenient for subsequent detection and diagnosis.

[0016] Furthermore, the lower end surface of the connecting pipe is an inclined structure, so as to play a role of drainage in the process of transferring sediment and / or suspension, which is conducive to more convenient operation.

[0017] Preferably, the lower end of the necked portion is provided with an external thread, and the bottom cover is provided with an internal thread matched with the external thread; or, the lower end of the necked portion is provided with a slot, and the bottom cover is provided with a buckle matched with the slot, and the sample discharge port is closed by the cooperation of the buckle and the slot, so as to control the opening and closing of the sample discharge port.

[0018] Preferably, the storage portion is made of transparent material.

[0019] Preferably, the storage portion is made of glass or plastic.

[0020] For the convenience of measurement, preferably, scale lines are provided along the height direction of the bottle body.

[0021] In order to facilitate hanging the precipitation bottle, it further includes a hanging part, which is arranged on the sealing member or the bottle body for hanging. When taking out the cell suspension and / or sediment in the precipitation bottle, the user can hang the precipitation bottle on the bracket by using the hanging part, so as to facilitate taking out the cell suspension and / or sediment from the discharge port below.

[0022] In order to conveniently and safely handle the supernatant after precipitation, further, a recovery part for storing the supernatant is further provided on the outer side of the necked part, the recovery part has a closed storage cavity, and the volume of the storage cavity is greater than or equal to the volume of the internal cavity, and is used to store the supernatant; an annular protrusion is provided on the side of the bottle body, and first lock buckles are respectively provided on both sides of the annular protrusion, and the annular protrusion is also provided with a first communicating hole, the first communicating hole is located between the two first lock buckles, and an annular groove is provided on the outer side of the annular protrusion pair, an annular sealing ring is fixed in the annular groove, the annular sealing ring is provided with a communicating port, and the communicating port is connected with the first communicating hole; the recovery part is an annular structure, and two second lock buckles respectively matched with the first lock buckles are provided on the inner side of the recovery part, the adjusting part is a second communicating hole provided between the two second lock buckles, the second communicating hole is connected with the storage cavity, the recovery part is connected to the annular protrusion through the cooperation of the second lock buckle and the first lock buckle, and the annular sealing ring is in a compressed state along the radial direction. Not only can the effect of pressure sealing be achieved, but the relative position of the second communicating hole and the communicating port can also be adjusted by rotating the recovery part. When the second communicating hole is rotated to a position corresponding to the communicating port, the two are connected, so that the bottle body can be connected to the recovery part, so that the supernatant in the bottle body can automatically flow into the recovery part under the action of gravity, thereby achieving the purpose of storing the supernatant and avoiding arbitrary discharge. Then, the recovery part only needs to be rotated appropriately to make the second communicating hole and the communicating port misaligned, so as to achieve the purpose of sealing the recovery part, thereby preventing the supernatant from flowing out of the recovery part.

[0023] Preferably, the first lock buckle is an annular clamping block disposed on the upper and lower sides of the annular protrusion, and the second lock buckle is an annular buckle with an L-shaped cross section, and the annular buckle cooperates with the annular clamping block. Not only can the recovery part be fixed, but also the recovery part can be rotated relative to the bottle body under the action of external force, thereby realizing a movable connection.

[0024] To facilitate separation of the necked portion and the bottle body, an annular connector is further included. The lower end of the bottle body is provided with an external thread or an internal thread, and the annular connector is provided with an internal thread or an external thread that matches the external thread or internal thread, and the annular protrusion is provided on the annular connector.

[0025] In order to avoid the problem that the recovery part may rotate relative to the necking part during the process of connecting the necking part and the bottle body, thereby failing to tighten the necking part and the bottle body, in a further solution, the outer side surface of the annular connector is further provided with a first stopper, and the recovery part is provided with a second stopper matched with the first stopper, and the cooperation between the first stopper and the second stopper limits the recovery part to only rotate one circle relative to the annular connector. That is, the first stopper and the second stopper can interfere with each other, thereby achieving the purpose of limiting, and the second stopper can only rotate one circle relative to the necking part driven by the recovery part, and in this process, the second connecting hole and the connecting port can overlap once to achieve connection.

[0026] Compared with the prior art, the use of a pleural effusion precipitation bottle provided by the present invention has the following beneficial effects:

[0027] 1. This sedimentation bottle is not only convenient for the collection, storage and transfer of pleural and abdominal effusions, but also can conveniently remove the upper washing solution while fully retaining the cell suspension and / or sediment, so that the upper washing solution is separated from the target cells, thereby conveniently collecting the cell suspension and / or sediment for cytological detection.

[0028] 2. This sedimentation bottle has a simple and compact structure. It is not only easy to use, but also can effectively avoid biosafety problems caused by random discharge. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0030] Figure 1 This is a schematic diagram of the structure of a pleural and ascites effusion sedimentation bottle provided in Example 1 of the present invention.

[0031] Figure 2 for Figure 1 Bottom view of .

[0032] Figure 3 for Figure 1 A partial cross-sectional view of .

[0033] Figure 4 This is a schematic diagram of the structure of another pleural and abdominal effusion precipitation bottle provided in Example 1 of the present invention.

[0034] Figure 5 This is a schematic diagram of the partial structure of a pleural and ascites effusion sedimentation bottle provided in Example 1 of the present invention.

[0035] Figure 6 This is a schematic diagram of the structure of a pleural and ascites effusion sedimentation bottle provided in Example 1 of the present invention.

[0036] Figure 7 This is a schematic diagram of the structure of a pleural and ascites effusion sedimentation bottle provided in Example 2 of the present invention.

[0037] Figure 8 This is a schematic diagram of the structure of a pleural and ascites effusion sedimentation bottle provided in Example 3 of the present invention.

[0038] Fig. 9 for Figure 8 The partial cross-sectional view shows that the bottle body and the recovery part are in a connected state.

[0039] Fig.10 for Figure 8 A partial cross-sectional view of the bottle body and the recovery part is not connected.

[0040] Markings in the figure are as follows: bottle body 101, liquid inlet 102, sealing member 103, necking portion 104, sample discharge port 105, control member 106, liquid discharge port 107, valve 108, base 109, support sheet 110, scale line 111, annular boss 112, internal cavity 113, external thread 114; bottom cover 201, connecting tube 202; recovery portion 301, storage cavity 302, second connecting hole 303, second locking buckle 304, second stopper 305; annular connector 401, internal thread 402, first connecting hole 403, annular sealing ring 404, connecting port 405, first stopper 406, first locking buckle 407. DETAILED DESCRIPTION

[0041] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. The components of the embodiments of the present invention generally described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents the selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work belong to the scope of protection of the present invention.

[0042] Example 1

[0043] See also Figure 1-Figure 3In the present embodiment, a pleural effusion sedimentation bottle is provided, comprising a storage portion for accommodating pleural effusion, the storage portion is provided with an internal cavity 113 for accommodating pleural effusion, the upper portion of the storage portion is provided with a liquid inlet 102 for loading pleural effusion and a sealing member 103 for detachably sealing the liquid inlet 102, the bottom of the storage portion is provided with a sample discharge port 105 for discharging target cells and a control member 106 for controlling the opening / closing of the sample discharge port 105, the side of the lower portion of the storage portion is provided with a liquid discharge port 107 for discharging supernatant and a regulating portion for controlling the on / off of the liquid discharge port 107, in the present embodiment, the regulating portion preferably adopts a valve 108, the liquid inlet 102, the sample discharge port 105 and the liquid discharge port 107 are respectively connected to the internal cavity 113, as shown in FIG. Figure 3 shown.

[0044] When using the present sedimentation bottle, the collected pleural and ascites fluid can be added into the internal cavity 113 of the storage part through the liquid inlet 102, and sealed by the sealing member 103, which is convenient for preserving the pleural and ascites fluid and preventing the pleural and ascites fluid from being contaminated; when the present sedimentation bottle is sent to the pathology department, by letting the present sedimentation bottle stand still, the cell suspension and / or sediment (i.e., target cells) in the pleural and ascites fluid can be automatically deposited to the bottom of the sedimentation bottle, and after being still for a prescribed time, the regulating member is opened, and the supernatant can be conveniently discharged from the liquid discharge port 107 without tilting or turning over the sedimentation bottle, and the cell suspension and / or sediment can be retained at the bottom of the internal cavity 113, and will not be discharged as the sedimentation bottle is tilted or turned over; finally, the liquid inlet 102 can be conveniently opened through the control member 106, so that the cell suspension and / or sediment can be conveniently taken out from the present sedimentation bottle for cytological detection. The use of the sedimentation bottle is not only convenient for the collection, storage and transfer of pleural and abdominal effusions, but also can conveniently remove the upper washing solution while fully retaining the cell suspension and / or sediment, so that the upper washing solution is separated from the target cells, thereby conveniently collecting the cell suspension and / or sediment for cytological testing.

[0045] like Figure 2 and Figure 3 As shown, in a solution provided in this embodiment, the storage part includes a bottle body 101, the liquid inlet 102 is arranged at the upper part of the bottle body 101, the lower end of the bottle body 101 is provided with a necking part 104 with a gradually decreasing inner diameter, the sample discharge port 105 is arranged at the lower end of the necking part 104, the outer side of the necking part 104 is provided with a base 109 for supporting the bottle body 101, and the liquid discharge port 107 is arranged on the bottle body 101 or the necking part 104. The bottle body 101 can be stably placed on the desktop by providing the base 109, and the necking part 104 can be provided to facilitate the enrichment of the cell suspension and / or the precipitate in the necking part 104, and finally discharged from the necking part 104 to the present precipitation bottle.

[0046] The base 109 has a variety of implementations, as an example, Figure 2 and Figure 3 As shown, the base 109 is a cylindrical structure, for example, a cylindrical or conical cylindrical structure, etc. For the convenience of operation, as another example, as shown in 4, the base 109 includes at least three support pieces 110, and the support pieces 110 are respectively distributed along the circumferential direction of the bottle body 101, and the upper ends of the support pieces 110 are respectively connected to the bottle body 101 or the necking part 104, and the support pieces 110 are used to support the bottle body 101; it is convenient for the bottle body 101 to be placed stably on the desktop, and it is convenient for the user to operate the control member 106 through the gap between the two connected support pieces 110, which is conducive to more convenient use.

[0047] The seal 103 has multiple implementations. As one implementation, the seal 103 can be a rubber stopper. When in use, the rubber stopper is pressed into the liquid inlet 102 to achieve the purpose of closing the liquid inlet 102; as another implementation, the seal 103 can be a sealing cover, the sealing cover is provided with a threaded hole, the threaded hole is provided with an internal thread, and the liquid inlet 102 is provided with an external thread matched with the internal thread, which can achieve both sealing and detachable connection, and is more convenient to use; it can be understood that the seal 103 can also be a sealing cover, and the sealing cover is provided with a buckle, and the liquid inlet 102 is provided with a slot, and the sealing of the liquid inlet 102 is achieved by the cooperation of the buckle and the slot. The principle is the same as Lock and Lock, and it will not be repeated here.

[0048] In order to facilitate the discharge of the cell suspension and / or sediment at the bottom of the internal cavity 113, in the embodiment, the control member 106 includes a bottom cover 201, and the bottom cover 201 is detachably fixed to the sample discharge port 105, so that when in use, the user can open the sample discharge port 105 by opening the bottom cover 201 to discharge the cell suspension and / or sediment. Similarly, the bottom cover 201 has multiple implementations. As an example, the lower end of the necked portion 104 is provided with an external thread, and the bottom cover 201 is provided with an internal thread that matches the external thread. The sample discharge port 105 is detachably closed by the cooperation of the external thread and the internal thread, such as Figure 2 and Figure 3 As shown, as another example, a slot is provided at the lower end of the necked portion 104, and the bottom cover 201 is provided with a buckle matched with the slot. The sample discharge port 105 can also be closed by the cooperation between the buckle and the slot, which will not be described in detail here.

[0049] Preferably, in this embodiment, the storage part is made of a transparent material, such as glass or plastic. To facilitate measurement, a scale line 111 is provided along the height direction of the bottle body 101. Figure 3 shown.

[0050] In this embodiment, since the discharge port 105 is arranged at the lower end of the entire sedimentation bottle, there is usually an inconvenient operation problem when taking out the cell suspension and / or sediment. In a further scheme, the sedimentation bottle also includes a hanging portion, which is arranged on the sealing member 103 or the bottle body 101 and is used for hanging. When taking out the cell suspension and / or sediment in the sedimentation bottle, the user can use the hanging portion to hang the sedimentation bottle on the bracket, thereby facilitating the removal of the cell suspension and / or sediment from the discharge port 105 below.

[0051] As an example, the hanging portion may be a pull ring, and both ends of the pull ring are movably connected to the sealing member 103 or the bottle body 101 so as to be hung on the bracket; as another example, the hanging portion may be an annular boss 112 arranged along the circumferential direction of the bottle body 101, such as Figure 5 As shown, the bottle body 101 can be clamped on the bracket by the annular boss 112, so as to achieve the purpose of hanging.

[0052] Example 2

[0053] In order to facilitate the smooth removal of a large volume of sediment from the bottle body 101, the main difference between the present embodiment 2 and the above-mentioned embodiment 1 is that in the sedimentation bottle provided in the present embodiment, the necking portion 104 is detachably fixed to the lower end of the bottle body 101, and the drain port 107 is provided at the necking portion 104; Figure 6 As shown, the necked portion 104 and the bottle body 101 are connected in a detachable manner, which facilitates the separation of the necked portion 104 from the bottle body 101. Not only can the bottle body 101 be conveniently separated separately, but also a larger volume of sediment can be conveniently taken out from the upper end of the necked portion 104 (which can meet the demand that some sediment is not convenient to be discharged from the sample discharge port 105), which is easy to use.

[0054] In order to realize the detachable connection, there are various implementation methods. As an example, the lower end of the bottle body 101 is provided with an external thread, and the upper end of the necked portion 104 is provided with an internal thread adapted to the external thread, such as Figure 6 As shown, the detachable connection is achieved by the cooperation of the external thread and the internal thread, and the necked portion 104 and the bottle body 101 together enclose the internal cavity 113 .

[0055] Example 3

[0056] The main difference between this embodiment 3 and the above-mentioned embodiment 2 is that in the precipitation bottle provided in this embodiment, the bottom cover 201 is also provided with a connecting port, such as Figure 7As shown, the communication port is provided with a communication pipe 202, and the communication pipe 202 is provided with a valve 108 to control the opening / closing of the communication port. In actual use, when the sediment at the bottom of the sedimentation bottle needs to be taken out, the user can first open the valve 108, and use the communication port to drain the liquid around the sediment, so that only the sediment is left at the bottom of the sedimentation bottle, and then the user closes the valve 108 and then opens the bottom cover 201, so that the sediment with less water content (i.e., relatively dry sediment) can be obtained, which is more convenient for subsequent detection and diagnosis.

[0057] In order to facilitate the pouring of the sediment and suspension collected at the bottom of the sedimentation bottle into the test tube, Figure 7 As shown, the lower end surface of the connecting pipe 202 is an inclined structure so as to play a drainage role in the process of transferring the sediment and / or suspension, which is conducive to more convenient operation.

[0058] It can be understood that the connecting pipe 202 can be a hard pipe or a soft pipe.

[0059] Example 4

[0060] In the prior art, the supernatant separated from the collected pleural and abdominal fluids is usually directly poured out. Since the supernatant must contain components such as the patient's cells, there is a biosafety problem, and the prior art does not provide any solution. To solve this problem, the main difference between this embodiment and the above-mentioned embodiments 1 or 2 or 3 is that, in the precipitation bottle provided in this embodiment, a recovery part 301 for storing the supernatant is also provided on the outer side of the necked portion 104, and the recovery part 301 has a closed storage cavity 302, and the volume of the storage cavity 302 is greater than or equal to the volume of the internal cavity 113, so as to store the supernatant.

[0061] To control the communication between the recovery part 301 and the bottle body 101, as Figure 8-Figure 10 As shown, in one scheme, an annular protrusion is provided on the side of the bottle body 101, and first locking buckles 407 are respectively provided on both sides of the annular protrusion, and the annular protrusion is also provided with a first connecting hole 403, the first connecting hole 403 is located between the two first locking buckles 407, and an annular groove is provided on the outer side of the annular protrusion, an annular sealing ring 404 is fixed in the annular groove, and the annular sealing ring 404 is provided with a connecting port 405, and the connecting port 405 is connected with the first connecting hole 403.

[0062] The recovery part 301 is an annular structure, and two second lock buckles 304 respectively matched with the first lock buckles 407 are arranged on the inner side of the recovery part 301. The adjustment part is a second connecting hole 303 arranged between the two second lock buckles 304, and the second connecting hole 303 is connected with the storage cavity 302. The recovery part 301 is connected to the annular protrusion through the cooperation of the second lock buckle 304 and the first lock buckle 407, and the annular sealing ring 404 is in a compressed state in the radial direction, which can not only achieve the effect of pressure sealing, but also adjust the relative position of the second connecting hole 303 and the connecting port 405 by rotating the recovery part 301. When the second connecting hole 303 is rotated to the position corresponding to the connecting port 405, the two are connected, so that the bottle body 101 can be connected to the recovery part 301.

[0063] The first lock buckle 407 and the second lock buckle 304 can be implemented in a variety of ways. For example, Figure 8 or Fig. 9 or Fig.10 As shown, the first lock buckle 407 is an annular clamping block arranged on the upper and lower sides of the annular protrusion, and the second lock buckle 304 is an annular buckle with an L-shaped cross-section. The annular buckle can cooperate with the annular clamping block, which can not only fix the recovery part 301, but also enable the recovery part 301 to rotate relative to the bottle body 101 under the action of external force, thereby realizing a movable connection.

[0064] In order to facilitate the separation of the necking portion 104 and the bottle body 101, in a further embodiment, an annular connector 401 is further included. The lower end of the bottle body 101 is provided with an external thread 114 or an internal thread 402. The annular connector 401 is provided with an internal thread 402 or an external thread 114 that matches the external thread 114 or the internal thread 402, so as to achieve threaded connection. The annular protrusion is provided on the annular connector 401. Fig. 9 or Fig.10 shown.

[0065] In order to avoid the problem that the recovery part 301 may rotate relative to the necking part 104 during the process of connecting the necking part 104 and the bottle body 101, thereby failing to tighten the necking part 104 and the bottle body 101, in a further solution, the outer side of the annular connector 401 is further provided with a first stopper 406, and the recovery part 301 is provided with a second stopper 305 adapted to the first stopper 406, and the cooperation between the first stopper 406 and the second stopper 305 restricts the recovery part 301 to only rotate relative to the necking part 104. When the annular connector 401 rotates one circle, for example, the first stopper 406 and the second stopper 305 can be respectively arranged in the same plane perpendicular to the center line of the bottle body 101, so that the first stopper 406 and the second stopper 305 can interfere with each other, thereby achieving the purpose of limiting, that is, the second stopper 305 can only rotate one circle relative to the necking portion 104 driven by the recovery portion 301. During this process, the second connecting hole 303 and the connecting port 405 can overlap once to achieve connection.

[0066] The method of using the precipitation bottle provided in this embodiment is:

[0067] 1. Rotate the recovery part 301 so that the second stopper 305 is located between the first stopper 406 and the connecting port 405 along the circumferential direction, and in the process of tightening the recovery part 301 and the bottle body 101, the second stopper 305 rotates in a direction away from the connecting port 405. At this time, the internal cavity 113 is not connected with the storage cavity 302, but is connected with the necking part 104.

[0068] 2. Add the collected pleural and abdominal fluid into the internal cavity 113 of the bottle body 101 and let it stand for sedimentation so that the cell suspension and / or precipitate to be collected can settle into the necked portion 104 .

[0069] 3. After the sedimentation is completed, the recovery part 301 is rotated in the opposite direction relative to the bottle body 101 (i.e., the rotation direction is opposite to that when it is initially tightened). Within one circle, the second connecting hole 303 will inevitably rotate to a position corresponding to the connecting port 405, so that the two are connected, so that the bottle body 101 can be connected to the recovery part 301, and the supernatant in the bottle body 101 can automatically enter the storage cavity 302 of the recovery part 301 under the action of gravity.

[0070] 4. After all the supernatant in the bottle body 101 enters the recovery part 301, continue to rotate the recovery part 301 so that the second connecting hole 303 and the connecting port 405 are misaligned, thereby achieving the purpose of sealing the storage cavity 302, preventing the internal liquid from flowing out, thereby preventing the supernatant from being discharged at will, and effectively solving the biosafety problem.

[0071] 5. Continue to rotate the recovery part 301 until the second stopper 305 contacts the first stopper 406, and then continue to rotate the recovery part 301. At this time, the recovery part 301 will drive the annular connector 401 relative to the bottle body 101, so that the necking part 104, the annular connector 401 and the recovery part 301 can be separated from the bottle body 101 as a whole, so that it is convenient to remove the sediment from the upper part of the necking part 104, or discharge it from the discharge port at the lower part of the necking part 104, which is very convenient. The necking part 104, the annular connector 401, the recovery part 301 and the supernatant sealed in the recovery part 301 after use can be used as a whole for subsequent waste treatment, which is very environmentally friendly and can effectively avoid biosafety problems caused by arbitrary discharge.

[0072] It can be understood that the recovery part 301 has various shapes, such as a ring, etc. Figure 8 As shown, the base 109 can also be directly arranged at the lower end of the recovery part 301, which will not be repeated here.

[0073] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A precipitation bottle, characterized in that: The storage device comprises a storage part for accommodating pleural and ascites, the storage part is provided with an internal cavity for accommodating pleural and ascites, the upper part of the storage part is provided with a liquid inlet for loading pleural and ascites and a sealing member for detachably closing the liquid inlet, the bottom of the storage part is provided with a sample discharge port for discharging target cells and a control member for controlling the opening / closing of the sample discharge port, the side of the lower part of the storage part is provided with a liquid discharge port for discharging supernatant and a regulating member for controlling the opening / closing of the liquid discharge port, and the liquid inlet, the sample discharge port and the liquid discharge port are respectively connected to the internal cavity; The storage part comprises a bottle body, the liquid inlet is arranged at the upper part of the bottle body, the lower end of the bottle body is provided with a necking part with a gradually decreasing inner diameter, the sample discharge port is arranged at the lower end of the necking part, and the outer side of the necking part is provided with a base for supporting the bottle body; scale lines are arranged along the height direction of the bottle body; The outer side of the necked portion is also provided with a recovery portion for storing supernatant, the recovery portion has a closed storage cavity, and the volume of the storage cavity is greater than or equal to the volume of the internal cavity, and is used to store supernatant; an annular protrusion is provided on the side of the bottle body, and first lock buckles are respectively provided on both sides of the annular protrusion, and the annular protrusion is also provided with a first communicating hole, the first communicating hole is located between the two first lock buckles, and an annular groove is provided on the outer side of the annular protrusion, an annular sealing ring is fixed in the annular groove, the annular sealing ring is provided with a communicating port, and the communicating port is connected with the first communicating hole; the recovery portion is an annular structure, and two second lock buckles respectively matched with the first lock buckles are provided on the inner side of the recovery portion, the adjusting portion is a second communicating hole provided between the two second lock buckles, the second communicating hole is connected with the storage cavity, the recovery portion is connected to the annular protrusion through the cooperation of the second lock buckle and the first lock buckle, and the annular sealing ring is in a compressed state in the radial direction, and the recovery portion can rotate relative to the bottle body under the action of external force.

2. The precipitation bottle according to claim 1, characterized in that: The base is a cylindrical structure; Alternatively, the base includes at least three support plates, which are respectively distributed along the circumferential direction of the bottle body, and the upper ends of the support plates are respectively connected to the necked portions, and the support plates are used to support the bottle body.

3. The precipitation bottle according to claim 1, characterized in that: The sealing member is a rubber stopper; Alternatively, the sealing member is a sealing cover, the sealing cover is provided with a threaded hole, the threaded hole is provided with an internal thread, and the liquid inlet is provided with an external thread that matches the internal thread.

4. The precipitation bottle according to any one of claims 1 to 3, characterized in that: The control component comprises a bottom cover, and the bottom cover is detachably fixed at the sample discharge port.

5. The precipitation bottle according to claim 4, characterized in that: The bottom cover is also provided with a communication port, the communication port is provided with a communication pipe, and the communication pipe is provided with a valve.

6. The precipitation bottle according to claim 4, characterized in that: The lower end of the necked portion is provided with an external thread, and the bottom cover is provided with an internal thread matching the external thread; Alternatively, a slot is provided at the lower end of the necked portion, and a buckle matched with the slot is provided at the bottom cover, and the sample discharge port is closed by the cooperation between the buckle and the slot.

7. The precipitation bottle according to claim 1, characterized in that: It also includes a hanging portion, which is arranged on the sealing member or the bottle body and is used for hanging.

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