A scattering automatic analysis device

Abstract

The present invention discloses a scattering automatic analysis device, which mainly includes: a light source component, a scattering light fixture body and a scattering light detection component, wherein the scattering light fixture body has a first placement end for placing the light source component, a second placement end for placing the scattering light detection component, and a test object placement area between the first placement end and the second placement end, wherein the light source component is placed on the first placement end, and the formed light path passes through the test object placement area; the scattering light detection component is placed on the second placement end of the scattering light fixture body, and is located on the light path formed by the light source component and passing through the test object placement area, and detects the corresponding scattered light. The scattering automatic analysis device provided by the present invention can quantitatively analyze the concentration of the test liquid with high sensitivity by measuring the light quantity change of the scattered light that is easy to capture a larger light quantity change.

Description

Technical Field

[0001] The present invention relates to the technical field of automatic analysis devices for analyzing the amount of components contained in human body fluids, and in particular to a scattering automatic analysis device. Background Art

[0002] In the prior art, the following automatic analyzer is widely used to measure the content of a certain component in human body fluids. The automatic analyzer irradiates a reaction solution containing a mixture of a specimen such as human body fluids and a reagent with light from a light source, calculates the absorbance based on the change in the amount of transmitted light at a wavelength of 790, and quantifies the concentration of the measured substance according to the Lambert-Beer law.

[0003] The specific protein analyzer is an analytical instrument used to measure the content of specific proteins in human body fluids. It uses the principle of laser scattering turbidimetry to measure the turbidity of the solution after the reaction of the sample and reagent in the reaction cup to determine the level of specific protein content in the sample. The level of specific protein content reflects the corresponding state of the patient's body and provides effective reference data for doctors' diagnosis. The specific protein analyzer is a biochemical analyzer widely used in medical institutions.

[0004] In existing biochemical analyzers or specific protein analyzers, the transmission light path mainly uses a halogen lamp, which is then divided into different wavelengths by adding a grating or various filters, or by using optical fibers plus various filters to divide into different wavelengths.

[0005] The reaction solution containing the sample and reagents is irradiated with light on the automatic analyzer, and the change in the amount of light transmitted without scattering is measured to quantify the concentration of the substance to be measured in the sample. The higher the concentration of the substance to be measured, the greater the change in the amount of light. In recent years, as the demand for immunoassays has increased, the performance requirements for immunoassays have also become higher and higher.

[0006] However, the existing technology uses the method of measuring the concentration of a substance by not easily capturing the change in the amount of transmitted light, and cannot meet the existing measurement requirements.

[0007] Therefore, one of the problems that those skilled in the art urgently need to solve is to provide a method for quantifying concentration with high sensitivity by using the light intensity change of scattered light that can easily capture a larger light intensity change. Summary of the invention

[0008] In order to solve the problems existing in the existing testing technology, a new testing solution is needed.

[0009] Therefore, the problem to be solved by the present invention is to provide an automatic scattering analysis device to overcome the problems existing in the prior art.

[0010] In order to solve the above problems, the present invention provides an automatic scattering analysis device, which mainly includes: a light source assembly, a scattered light bench body and a scattered light detection assembly, wherein the scattered light bench body has a first placement end for placing the light source assembly, a second placement end for placing the scattered light detection assembly, and a test object placement area between the first placement end and the second placement end, the light source assembly is placed on the first placement end, and the formed light path passes through the test object placement area; the scattered light detection assembly is placed on the second placement end on the scattered light bench body, and is located on the light path formed by the light source assembly and passing through the test object placement area to detect corresponding scattered light.

[0011] Furthermore, the light source assembly includes a 790nm laser scattering lamp and a scattering lamp tube, the output end of the scattering lamp tube is arranged at the first placement end of the scattering light holder body, the 790nm laser scattering lamp is adjustably arranged at the incident end of the scattering lamp tube, and an adjustable spacing is left between the 790nm laser scattering lamp and the scattering lamp tube in the length direction.

[0012] Furthermore, the 790nm laser scattering lamp emits 790nm parallel light with a spot size of 2mm-5mm.

[0013] Furthermore, the area for placing the object to be tested on the scattered light fixture seat is an arc sector-shaped groove.

[0014] Furthermore, two horizontal through holes and an inclined through hole with the same diameter and an axial line forming an angle are provided on the second placement end of the scattered light fixture seat body. The horizontal through hole is coaxially arranged with the through hole on the scattering lamp tube, and the inclined through hole is used to place the scattered light detection component, and can guide the scattered light passing through the liquid to be tested to the scattered light detection component.

[0015] Furthermore, the scattered light detection assembly includes a focusing tube, a plano-convex lens, a filter, a rubber gasket, an optical bench circuit board support sleeve, a photoelectric colorimetric receiving circuit board and a shielding box, wherein the focusing tube is arranged in the inclined through hole of the scattered light bench seat body; the optical bench circuit board support sleeve is placed on the inclined through hole of the scattered light bench seat body and cooperates with the focusing tube to fix the focusing tube in the inclined through hole; the plano-convex lens and the filter are successively placed in the optical bench circuit board support sleeve and cooperate with the focusing tube to form an optical path; the photoelectric colorimetric receiving circuit board is placed in the optical bench circuit board support sleeve and abuts and cooperates with the filter through the rubber gasket; the shielding box is placed on the optical bench circuit board support sleeve.

[0016] Furthermore, one end of the optical bench line support sleeve is set as a cylindrical protruding end, and a stepped placement hole is set inside the optical bench line support sleeve. The upper placement hole is used to place a plano-convex lens and a filter, and the lower placement hole is used to place a photoelectric colorimetric receiving circuit board.

[0017] Furthermore, a protruding photosensitive device is provided at one end of the photoelectric colorimetric receiving circuit board, and the protruding photosensitive device is arranged in contact with the rubber gasket of the optical bench circuit support sleeve.

[0018] The automatic scattering analysis device provided by the present invention can achieve highly sensitive quantitative analysis of the concentration of the liquid to be tested by measuring the light quantity change of scattered light which is easy to capture greater light quantity change, and has simple operation and high test accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments.

[0020] Figure 1 Schematic diagram of the overall structure of the scattering automatic analysis device in this example;

[0021] Figure 2 is a schematic cross-sectional view of the scattering automatic analysis device in this example;

[0022] Figure 3 This is a schematic diagram of the structure of the scattered light seat of the scattered light automatic analysis device in this example;

[0023] Figure 4 It is a schematic diagram of the structure of the scattering lamp tube of the scattering automatic analysis device in this example.

[0024] Meaning of the symbols in the figure:

[0025] 790nm laser scattering lamp 1, scattering lamp tube 2, scattering light fixture seat body 3, groove 3a, first placement end 31, second placement end 32, optical fixture circuit board support sleeve 4, cylindrical protruding end 4a, upper placement hole 4c, lower placement hole 4b, shielding box 5, photoelectric colorimetric receiving circuit board 6, protruding photosensitive device 6a, rubber gasket 7, filter 8, plano-convex lens 9, focusing tube 10, scattering light fixture seat body horizontal through hole 11, scattering light fixture seat body inclined through hole 12, first adjustment threaded hole 13, first mounting threaded hole 14, second mounting threaded hole 15, second adjustment threaded hole 16. DETAILED DESCRIPTION

[0026] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below with reference to specific diagrams.

[0027] As the requirements for test performance in immunoassay projects become increasingly higher, the existing technology that uses light intensity changes that are not easy to capture to measure the concentration of a substance cannot meet existing measurement requirements.

[0028] This example provides a scattering automatic analysis device that can quantify concentration with high sensitivity by using the light quantity change of scattered light that can easily capture a larger light quantity change.

[0029] like Figure 1 , Figure 2 As shown, the scattering automatic analysis device mainly includes: a light source component, a scattering light holder body and a scattering light detection component.

[0030] Among them, the scattered light bench body is provided with a first placement end 31 for placing the light source component and a second placement end 32 for placing the scattered light detection component, and a to-be-tested object placement area is provided between the first placement end 31 and the second placement end 32. The light source component here is placed on the first placement end 31, and the formed light path passes through the to-be-tested object placement area; the scattered light detection component here is placed on the second placement end 32 on the scattered light bench body, and is located on the light path formed by the light source component and passing through the to-be-tested object placement area to detect the corresponding scattered light.

[0031] The light source assembly is mainly used to provide a scattered light source, and the scattered light seat is mainly used to guide the direction of the light source and guide the scattered light to the scattered light detection assembly, and the scattered light detection assembly then analyzes the detected scattered light to obtain the concentration value of the object to be detected.

[0032] Specifically, the light source assembly here is mainly composed of a 790nm laser scattering lamp 1 and a scattering lamp tube 2. The scattering lamp tube 2 is fixed on the first mounting end 31 of the scattering light fixture seat body 3. A through hole is opened in the scattering lamp tube 2 for mounting the 790nm laser scattering lamp 1.

[0033] The 790nm laser scattering lamp 1 here emits 790nm parallel light, and the spot size is 2mm-5mm, preferably set to 3mm. There is a gap between the 790nm laser scattering lamp 1 and the scattering lamp tube 2 in the length direction to adjust the energy value of the incident light, that is, the light intensity.

[0034] For details, see Figure 4 The front end of the scattering lamp tube 2, i.e., the end connected to the 790nm laser scattering lamp 1, is evenly distributed along its circumferential direction with a plurality of second adjustment threaded holes 16, and the 790nm laser scattering lamp 1 and the scattering lamp tube 2 are connected by screws.

[0035] The number of the second adjusting threaded holes 16 is not specifically limited here, and three are taken as an example in this embodiment.

[0036] Also, see Figure 3 The rear end of the scattering lamp tube 2 can be adjustably arranged on the scattering light fixture seat body 3.

[0037] In specific configuration, a first adjusting threaded hole 13 is further provided on the first placement end 31 of the scattered light fixture body 3, and the rear end of the scattered light tube 2 is fixedly connected to the first placement end 31 of the scattered light fixture body 3 by screws.

[0038] like Figure 1-Figure 3 As shown, the rear end length of the scattering lamp tube 2 and the first mounting end 31 connected to the scattering light fixture seat body 3 are both provided with sufficient thickness. Such a structural arrangement facilitates the adjustment of the length of the connecting portion between the two, thereby facilitating the adjustment of the light intensity of the incident light reaching the photoelectric colorimetric receiving circuit board 6.

[0039] The area for placing the object to be measured on the scattered light fixture seat body 3 is specifically configured as an arc sector-shaped groove 3a for placing the colorimetric device. The specific shape of the groove 3a can be configured according to the shape of the colorimetric device.

[0040] In addition, if Figure 2 As shown, on the second placement end 32 of the scattered light fixture seat body 3, there are two through holes with the same diameter of about 5 mm and the axis lines forming an angle range of 20-30 degrees, namely the horizontal through hole 11 of the scattered light fixture seat body and the inclined through hole 12 of the scattered light fixture seat body.

[0041] The horizontal through hole 11 of the scattered light fixture seat body is coaxial with the through hole on the scattered light tube 2 , and is used to guide the light in the scattered light tube 2 .

[0042] The inclined through hole 12 of the scattered light holder body is used to guide the scattered light to reach the scattered light detection component.

[0043] The scattered light detection component is used to analyze the scattered light passing through the colorimetric device containing the liquid to be tested, and mainly includes: a focusing tube 10, a plano-convex lens 9, a filter 8, a rubber gasket 7, an optical bench circuit board support sleeve 4, a photoelectric colorimetric receiving circuit board 6 and a shielding box 5.

[0044] The focusing tube 10 is used to focus the scattered light passing through the colorimetric device containing the liquid to be tested, so that the photoelectric colorimetric receiving circuit board 6 can detect the received scattered light.

[0045] The focusing tube 10 is embedded in the diffuser light fixture body 3, and is specifically arranged in the inclined through hole 12 of the diffuser light fixture body.

[0046] On the second mounting end 32 of the scattered light fixture body 3, there are also provided an optical fixture circuit board support sleeve 4, a photoelectric colorimetric receiving circuit board 6, and a shielding box 5, which are fixed together on the scattered light fixture body 3 through a first mounting threaded hole 14 and a second mounting threaded hole 15.

[0047] See also Figure 2 As shown, one end of the optical bench line support sleeve 4 is set as a cylindrical protruding end 4a, which is inserted into the scattered optical bench body 3 and is in contact with the focusing tube 10 embedded in the scattered optical bench body 3.

[0048] The optical bench line support sleeve 4 is provided with stepped placement holes inside. The upper placement hole 4c is used to place the plano-convex lens 9 and the filter 8, and the lower placement hole 4b is used to place the photoelectric colorimetric receiving circuit board 6.

[0049] Among them, the plano-convex lens 9, the filter 8 and the rubber gasket 7 of the optical bench circuit support sleeve 4 are arranged in sequence along the inner side of the upper placement hole 4c.

[0050] The plano-convex lens 9 is used to re-focus the light passing through the focusing tube 10, so as to facilitate the photoelectric colorimetric receiving circuit board 6 to detect the received scattered light.

[0051] The filter 8 is mainly used to filter the light of a specific wavelength required for detection by the photoelectric colorimetric receiving circuit board 6 .

[0052] The rubber gasket 7 is used to provide a protective function to avoid collision caused by contact between the two components.

[0053] Here, a protruding photosensitive device 6 a is disposed at one end of the photoelectric colorimetric receiving circuit board 6 , and the protruding photosensitive device 6 a is disposed in contact with the rubber gasket 7 of the optical bench circuit support sleeve 4 .

[0054] The rubber gasket 7 here protects the protruding photosensitive device 6a to avoid damage caused by contact with the front filter 8.

[0055] During specific testing, the inclined through hole 12 of the scattered light fixture body can guide the scattered light through the focusing tube 10 and the plano-convex lens 9 to the photoelectric colorimetric receiving circuit board 6 .

[0056] Furthermore, the shielding box 5 is mainly used to isolate unnecessary interference to the scattered light detection caused by the outside, and it and the stepped placement hole of the optical bench line support sleeve 4 can form a cavity, and the photoelectric colorimetric receiving circuit board 6 is arranged in the cavity.

[0057] Based on the scattering automatic analysis device with the above-mentioned structure, during the experiment, first adjust the 790nm laser scattering lamp 1 on the scattering light fixture seat body 3 to a suitable position in the scattering lamp tube 2 to ensure that the 790nm laser scattering lamp 1 and the scattering lamp tube 2 and the through hole in the scattering light fixture seat body 3 and the scattering lamp tube 2 are coaxial, then place the test sample in the groove 3a on the scattering light fixture seat body 3 to start the test.

[0058] Specifically, first, the 790nm laser scattering lamp 1, the scattering lamp tube 2 and the scattering light fixture seat body 3 are adjusted to be on the same axis through the first adjusting threaded hole 13 and the second adjusting threaded hole 16, and the direct connection length of the three is adjusted to obtain a suitable optical distance.

[0059] Then, the focusing tube 10 is installed into the inclined through hole 12 of the scattered light fixture body 3 .

[0060] Then, the plano-convex lens 9 , the filter 8 and the rubber gasket 7 are installed in sequence to the upper placement hole 4 a of the optical bench circuit board support sleeve 4 .

[0061] Then, the photoelectric colorimetric receiving circuit board 6 is fixed to the lower placement hole 4 b of the optical bench circuit board support sleeve 4 to support the rubber gasket 7 .

[0062] Finally, the shielding box 5 together with the optical bench circuit board support sleeve 4 with other components installed thereon are mounted on the diffuse optical bench seat body 3 through the first mounting threaded holes 14 and the second mounting threaded holes 15 .

[0063] After the above-mentioned scattering automatic analysis device is assembled, the 790nm laser scattering lamp 1 of the device is connected to a power source, and the photoelectric colorimetric receiving circuit board 6 is connected to a data processing device to start measurement.

[0064] During the initial measurement, no colorimetric device is placed in the scattering automatic analysis device, and the light emitted by the 790nm laser scattering lamp 1 directly passes through the horizontal through hole 11 of the scattering light fixture seat body and does not reach the photoelectric colorimetric receiving circuit board.

[0065] When a colorimetric device is placed in the automatic scattering analysis device, light scattering occurs when the light emitted by the 790nm laser scattering lamp 1 contacts particles of appropriate size in the colorimetric device, causing part of the light to pass through the inclined through hole 12 of the scattered light holder to reach the photoelectric colorimetric receiving circuit board 6, thereby obtaining the result value.

[0066] The automatic scattering analysis device provided by the present invention can achieve highly sensitive quantitative analysis of the concentration of the liquid to be tested by measuring the light quantity change of scattered light which is easy to capture greater light quantity change, and has simple operation and high test accuracy.

[0067] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A scattering automatic analysis device, characterized in that: The invention mainly comprises: a light source assembly, a scattered light bench seat body and a scattered light detection assembly, wherein the scattered light bench seat body has a first placement end for placing the light source assembly, a second placement end for placing the scattered light detection assembly, and a detection object placement area arranged between the first placement end and the second placement end, wherein the light source assembly is placed on the first placement end, and the formed light path passes through the detection object placement area; The scattered light detection assembly is arranged on the second placement end of the scattered light bench seat body, and is located on the optical path formed by the light source assembly and passing through the placement area of ​​the object to be tested, and detects the corresponding scattered light, and is used to perform scattered light analysis on the colorimetric device containing the liquid to be tested; the scattered light detection assembly includes: a focusing tube, a plano-convex lens, a filter, a rubber gasket, an optical bench circuit board support sleeve, a photoelectric colorimetric receiving circuit board and a shielding box, The focusing tube is used to focus the scattered light passing through the colorimetric device containing the liquid to be tested, and the photoelectric colorimetric receiving circuit board is used to detect the received scattered light; the focusing tube is embedded in the inclined through hole of the scattered light fixture seat body; the optical fixture circuit board support sleeve, the photoelectric colorimetric receiving circuit board, and the shielding box are arranged on the second placement end of the scattered light fixture seat body, and the three are fixedly arranged on the scattered light fixture seat body; One end of the optical bench circuit board support sleeve is inserted into the scattered light bench body and is arranged in contact with the focusing tube embedded in the scattered light bench body. The optical bench circuit board support sleeve is provided with a step-shaped placement hole inside. The upper placement hole is used to place a plano-convex lens and a filter, and the lower placement hole is used to place a photoelectric colorimetric receiving circuit board. The plano-convex lens, the filter and the rubber gasket of the optical bench circuit board support sleeve are arranged in sequence along the inner side of the upper placement hole. The plano-convex lens is used to focus the light passing through the focusing tube again, and is used for the photoelectric colorimetric receiving circuit board to detect the received scattered light. The filter is used to filter the light of a specific wavelength required for the detection of the photoelectric colorimetric receiving circuit board. The rubber gasket provides protection to avoid collision caused by contact between the two components.

2. The automatic scattering analysis device according to claim 1, characterized in that: The light source assembly includes a 790nm laser scattering lamp and a scattering lamp tube, the output end of the scattering lamp tube is arranged in the first placement end of the scattering light fixture seat body, the 790nm laser scattering lamp is adjustably arranged at the incident end of the scattering lamp tube, and an adjustable spacing is left between the 790nm laser scattering lamp and the scattering lamp tube in the length direction.

3. The automatic scattering analysis device according to claim 2, characterized in that: The 790nm laser scattering lamp emits 790nm parallel light with a spot size of 2mm-5mm.

4. The automatic scattering analysis device according to claim 1, characterized in that: The area for placing the object to be tested on the scattering light fixture seat is an arc sector-shaped groove.

5. The automatic scattering analysis device according to claim 1, characterized in that: The second placement end of the scattered light fixture seat body is also provided with two horizontal through holes and an inclined through hole with the same diameter and an axis line forming a through hole angle in the range of 20-30 degrees. The horizontal through hole is coaxially arranged with the through hole on the scattering lamp tube, and the inclined through hole is used to place a scattered light detection component, and can guide the scattered light passing through the liquid to be tested to the scattered light detection component.

6. The automatic scattering analysis device according to claim 1, characterized in that: One end of the photoelectric colorimetric receiving circuit board is provided with a protruding photosensitive device, and the protruding photosensitive device is arranged in contact with the rubber gasket of the optical bench circuit board supporting sleeve.

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