A virus-carrying sex trapping cage and a method for controlling lepidopteran pests using the same

Abstract

The invention discloses a sex trapping cage with virus and a method for controlling lepidopteran pests with the same. The trapping cage comprises a sunscreen film cover, a hook, a metal ring buckle, a conical net and a poison-carrying basin; the hook is connected to an upper cover frame; a metal ring buckle is hung at the bottom of the upper cover frame; two ends of the conical net are respectively fixed to the metal ring buckle and the lower cover frame; the poison-carrying basin is hung at the bottom of the lower cover frame, and a sponge block placed in the poison-carrying basin is soaked with virus liquid; and a lure core is hung at the bottom of the metal ring buckle. During the crop growth period, more than two poison-carrying trapping cages are placed in each mu of field, which can effectively control the harm of lepidopteran pests, reduce the amount of chemical pesticides applied, and significantly reduce the cost of pest control. The organic combination of sex pheromone trapping and virus infection, two pest control measures, is achieved, the mating probability of male and female insects is reduced, and the male insect can take the virus liquid into the body, so that the infected male insect mates with the female insect, and the virus is vertically transmitted in the population, thereby achieving precise control of physical and biological control.

Description

Technical Field

[0001] The invention relates to the technical field of pest control, and in particular to a sex trapping cage with viruses and a method for controlling lepidopteran pests using the same. Background Art

[0002] Lepidoptera pests are important pests in agricultural production. They feed on stems and leaves, causing serious nicks or even bare stems on plants, resulting in reduced crop yields and crop failure. For the prevention and control of Lepidoptera pests, farmers currently still mainly use chemical control, with a large number of pesticide applications, and the interval is generally 5-7 days, which can easily cause excessive pesticide residues and reduced crop quality. It has been reported that Lepidoptera pests have developed resistance to major control agents such as avermectin, cypermethrin, emamectin benzoate, chlorfenapyr, and spinetoram. How to achieve the purpose of reducing the amount of chemical pesticides and controlling pests, and effectively use other comprehensive control measures, is an urgent problem to be solved in the prevention and control of Lepidoptera pests in agricultural production.

[0003] Physical and chemical control and biological control are currently the most effective and environmentally friendly means of pest control. The two control methods are organically combined to achieve precise control of Lepidoptera pests, thereby significantly reducing the field population of Lepidoptera pests and achieving the goal of minimizing the use of chemical pesticides. Insect pheromone trapping uses the volatile courtship chemical components secreted by female insects to lure male insects for hunting and killing, thereby reducing the chance of mating between male and female insects and reducing the population of the next generation; after insect pathogens (viruses, pathogenic fungi, etc.) infect the bodies of Lepidoptera pests, they can spread between populations, causing a sharp decline in populations and maintaining the population at a low level. At present, most of the literature studies are on the combination of pheromones and viruses to control Lepidoptera pests, but how to make adults carry viruses effectively is a difficult point in application. Currently, the main reports use powders as virus carriers, and Lepidoptera adults with piercing-sucking mouthparts cannot feed into the body, nor can they achieve vertical transmission of viruses within the population. Summary of the invention

[0004] The purpose of the present invention is to overcome the defects and shortcomings of the prior art and provide a sex trapping cage with viruses, which can be taken by male insects to take virus liquid into the body, has a simple overall structure and low cost.

[0005] Another object of the present invention is to provide a method for controlling lepidopteran pests using sex traps carrying viruses.

[0006] The purpose of the present invention can be achieved through the following technical scheme: a virus-carrying sex trapping cage, comprising a sunscreen film cover, a hook, a metal ring buckle, a conical net and a virus-carrying basin; the sunscreen film cover comprises an upper cover frame, a lower cover frame and a sunscreen film covering the upper cover frame and the lower cover frame; the hook is connected to the middle of the upper cover frame; a metal ring buckle is hung at the center bottom of the upper cover frame; one end of the conical net is fixed to the metal ring buckle, and the other end is fixed to the lower cover frame; the virus-carrying basin is hung at the bottom of the lower cover frame, a sponge block is placed in the virus-carrying basin, and the sponge block is soaked in virus liquid; an attractant core is hung at the bottom of the metal ring buckle.

[0007] Furthermore, the poison-carrying basin is hung on the bottom of the lower cover frame by a plurality of hanging rods made of iron wire.

[0008] Furthermore, the sunscreen film is a blue drip-free film with a thickness of 0.08 mm to 0.12 mm. The blue drip-free film has the properties of light transmission, heat preservation, rainproof and sunscreen, and can increase the service life of the trapping cage.

[0009] Furthermore, the attractant core is a sex pheromone of female adults of lepidopteran pests, such as the sex pheromone component attractant core of female adults of lepidopteran pests such as Spodoptera litura, Spodoptera exigua, rice borer, tobacco budworm, etc.

[0010] Furthermore, the conical net is a nylon net with a mesh density of 60 meshes.

[0011] Furthermore, the sponge block is a medium-density sponge with a length of 9 cm, a width of 9 cm and a height of 4 cm.

[0012] Furthermore, the concentration range of the virus solution is 10 7 Up to 10 8 PIB / mL.

[0013] Furthermore, the inner diameter of the metal ring buckle is 17 mm.

[0014] Another object of the present invention can be achieved by the following technical solution: A method for controlling lepidopteran pests using a sex trapping cage with a virus comprises the following steps:

[0015] Hang the bait core on the metal ring buckle, soak the sponge block with virus solution and place it in the virus basin;

[0016] Fix the trap cage in the field, keeping the bottom of the trap cage 1m to 1.5m from the ground or flush with the top of the crop leaves.

[0017] Furthermore, two trapping cages were arranged per acre of land.

[0018] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0019] 1. The trapping cage of the present invention has a simple structure and low production cost, and its selling price is only 1 / 3 of the price of similar sex trapping devices on the market. It realizes the organic combination of sex pheromone trapping and virus infection, reduces the mating probability of male and female insects, and male insects can take virus liquid into their bodies, so that after the infected male insects mate with female insects, the virus is vertically transmitted within the population, greatly improving the prevention and control effect, and realizing the precise prevention and control of physical and biological control.

[0020] 2. The trapping cage of the present invention can be used to prevent and control lepidopteran pests, such as the armyworm, beet armyworm, rice borer, tobacco budworm, etc., significantly reducing the number of pest populations, especially the production of female insects, and reducing the hatching rate of eggs and the survival rate of hatched larvae, thereby greatly reducing the number of pesticide applications and the amount of use.

[0021] 3. The trapping cage of the present invention is also provided with a sun-proof film, which can be used normally in the field for more than 3 years, is rainproof and sun-proof, and is more easily accepted by users. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Schematic diagram of the structure of the trapping cage in the embodiment of the present invention.

[0023] Including: 1: upper cover frame, 2: lower cover frame, 3: sun protection film, 4: hook, 5: metal ring buckle, 6: cone net, 7: poison basin, 8: sponge block, 9: bait core, 10: hanging rod. DETAILED DESCRIPTION

[0024] The present invention is further described in detail below in conjunction with embodiments and drawings, but the embodiments of the present invention are not limited thereto.

[0025] like Figure 1 As shown, a sex trapping cage with virus includes a sunscreen film cover, a hook 4, a metal ring buckle 5, a conical net 6 and a virus-carrying basin 7. The sunscreen film cover includes an upper cover frame 1, a lower cover frame 2 and a sunscreen film 3 covering the upper cover frame and the lower cover frame. In this embodiment, the upper cover frame 1 is an iron ring, and a vertical iron wire rod is provided in the middle to facilitate the fixing of the hook 4. The hook can also be made of iron wire and fixed in the middle of the upper cover frame, so that the trapping cage can be hung in the field. The lower cover frame 2 is also an iron ring, and a sunscreen film is covered between the upper cover frame and the lower cover frame. The top of the sunscreen film completely covers the top of the upper cover frame and is fixed to the edge of the upper cover frame, and the bottom of the sunscreen film is fixed to the edge of the ring of the lower cover frame, so that the upper cover frame and the lower cover frame are connected by the sunscreen film, and a cylindrical sunscreen space is formed. The sunscreen film is preferably a blue drip-free film with a thickness of 0.08 mm to 0.12 mm, which has the properties of light transmission, heat preservation, rain protection and sun protection, and can be used normally in the field for more than 3 years.

[0026] A metal ring 5 is hung at the bottom center of the upper cover frame. The inner diameter of the metal ring is 17 mm and is used to hang the bait core. The bait core can be selected from bait cores containing sex pheromone components of female adults of Lepidoptera pests such as Spodoptera litura, Spodoptera exigua, rice borer, and tobacco budworm. One end of the conical net 6 is fixed to the metal ring, and the other end is fixed to the lower cover frame. The conical net is preferably a nylon net with a mesh density of 60 meshes. The poison basin 7 is suspended from the bottom of the lower cover frame by a plurality of hanging rods 10 made of iron wire. A sponge block 8 is placed in the poison basin, and the sponge block 8 is soaked in virus liquid. The sponge block is preferably a medium-density sponge with a length of 9 cm, a width of 9 cm, and a height of 4 cm. The concentration of the virus liquid soaked in the sponge block is 10 7 Up to 10 8 PIB / mL.

[0027] The use principle of the trapping cage of the present invention is as follows: the trapping core is hung on the metal ring buckle, a sponge block is soaked with virus liquid and placed in a virus-carrying basin, the trapping cage hook is fixed on a bamboo pole, and is obliquely inserted into the field, the bottom of the trapping cage is kept 1m to 1.5m away from the ground or the bottom of the trapping cage is flush with the top of the crop leaves, and two trapping cages are arranged per mu of land.

[0028] Embodiment 1

[0029] Select a 1.8-meter-wide yurt mosquito net, put 15 pairs of male and female insects that have emerged one day into each mosquito net, and prepare 5×10 7 PIB / mL of Spodoptera litura nuclear polyhedrosis virus (Xu Shulan, Tang Li. Production process of 30 billion PIB / g Spodoptera litura nuclear polyhedrosis virus stock drug [J]. Guangdong Agricultural Science, 2011, 38 (16): 68-70), the lure is a special lure for Spodoptera litura, and the following four treatments are tested: (a) no trap cage + 5% honey water, (b) no trap cage + virus feeding liquid, (c) trap cage + 5% honey water, (d) trap cage + virus feeding liquid, each treatment is repeated three times, and the amount of female insects laying eggs is observed and recorded every day after the treatment until the female insects finish laying eggs, and the number of male insects captured by the trap cage is recorded at the same time. Take the egg masses of each treatment and each repeat, and observe the hatching rate of eggs and the survival rate of hatched larvae before the third instar.

[0030] As shown in Table 1, the average egg laying amount of the virus trapping cage treatment was 2898 eggs / 15 female insects, which was significantly lower than that of other treatments, but the difference in egg hatching rate among the treatments was not significant. The average survival rates of the hatched larvae in the two treatments with virus liquid were 47.78% and 55.56%, respectively, which were significantly lower than those in the treatment without virus liquid. The average number of male insects trapped in the two treatments of hanging trapping cages was 5.3 and 5.7, respectively, indicating that the hanging trapping cage can effectively trap male insects, reduce the mating probability of male and female insects, and the trapping rate of male insects is about 35%. It shows that the virus trapping cage treatment can effectively reduce the population of Spodoptera litura.

[0031] Table 1 Effects of different treatments on egg laying and egg development of female Spodoptera litura

[0032]

[0033]

[0034] Embodiment 2

[0035] A tobacco field with an area of ​​about 50 mu was selected. The tobacco seedling stage was one month after transplanting. Two sex pheromone traps with Spodoptera litura polyhedrosis virus solution were placed per mu. The bottom of the cage was about 1.2 meters above the ground. The virus solution (5×10 7 PIB / mL), and a tobacco field 1 km away from the test area was used as the control treatment area. Five random points were surveyed in the test area and the control area, and five tobacco plants were surveyed at each point. The number of Spodoptera litura larvae was surveyed before treatment and 10 days, 20 days and 30 days after treatment, and the average insect population reduction rate and control effect were calculated according to formulas (1) and (2).

[0036]

[0037]

[0038] As shown in Table 2, the virus-carrying traps have a good control effect on tobacco field Spodoptera litura, and the control effects are 87.01% and 85.48% 20 days and 30 days after treatment, respectively.

[0039] Table 2 Control effect of virus-carrying traps on tobacco armyworm

[0040]

[0041] Embodiment 3

[0042] A rice field with an area of ​​about 50 mu was selected. The rice growth period was the tillering stage. Two sex pheromone traps with cabbage armyworm polyhedrosis virus (3 billion PIB / mL suspension, produced by Jiangxi Xinlong Biotechnology Co., Ltd.) were placed in each mu. The lure core was a special lure core for the striped stem borer. The cage bottom was level with the tip of the rice leaf. The virus liquid (5×10 7 PIB / mL), and a rice field 1 km away from the experimental area was used as the control area. Five points were randomly surveyed in the experimental area and the control area, and each point was surveyed for 35m 2 , investigate the dead heart plants caused by Chilo suppressalis and calculate the relative control effect. The calculation formulas are shown in (3) and (4). Investigate the rice leaf roller before treatment and 10 days, 20 days and 30 days after treatment, and calculate the insect population reduction rate and control effect.

[0043]

[0044]

[0045] The control effect of the virus-carrying traps on rice stem borer is shown in Table 3. The control effects 20 and 30 days after treatment were 77.27% and 83.61%, respectively, showing good control effect and lasting field population control effect.

[0046] Table 3 Control effect of virus-carrying traps on rice stem borer

[0047]

[0048] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A sex trapping cage with virus, characterized in that: The invention comprises a sunscreen film cover, a hook, a metal ring buckle, a cone-shaped net and a virus-carrying basin; the sunscreen film cover comprises an upper cover frame, a lower cover frame and a sunscreen film covering the upper cover frame and the lower cover frame; the hook is connected to the middle of the upper cover frame; a metal ring buckle is hung at the center bottom of the upper cover frame; one end of the cone-shaped net is fixed to the metal ring buckle, and the other end is fixed to the lower cover frame; the virus-carrying basin is hung at the bottom of the lower cover frame, a sponge block is placed in the virus-carrying basin, and the sponge block is soaked with virus liquid; an attractant core is hung at the bottom of the metal ring buckle; The poison-carrying basin is hung on the bottom of the lower cover frame by a plurality of hanging rods made of iron wire; The sunscreen film is a blue drip-free film with a thickness of 0.08 mm to 0.12 mm; The conical net is a nylon net with a mesh density of 60 meshes; The concentration range of the virus solution is 10 7 Up to 10 8 PIB / mL.

2. A virus-carrying sex trapping cage according to claim 1, characterized in that: The attractant core is the sex pheromone of female adult lepidopteran pests.

3. A virus-carrying sex trapping cage according to claim 1, characterized in that: The sponge block is a medium-density sponge with a length of 9 cm, a width of 9 cm and a height of 4 cm.

4. A virus-carrying sex trapping cage according to claim 1, characterized in that: The inner diameter of the metal ring buckle is 17 mm.

5. A method for controlling lepidopteran pests using the virus-carrying sex trap cage according to any one of claims 1 to 4, characterized in that: The steps include: Hang the bait core on the metal ring buckle, soak the sponge block with virus solution and place it in the virus basin; Fix the trap cage in the field, keeping the bottom of the trap cage 1m to 1.5m from the ground or flush with the top of the crop leaves.

6. The method for controlling lepidopteran pests using a sex trapping cage with a virus according to claim 5, characterized in that: Two trapping cages were set up per acre.

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