Fresh handling method of radix isatidis medicinal material

Abstract

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to a fresh handling method of radix isatidis medicinal materials. The fresh handling method of radix isatidis medicinal materials comprises the following steps: storing fresh radix isatidis at 0-10 DEG C for 3-14 days; and vacuum drying the stored radix isatidis at 50-70 DEG C. The fresh handling method of radix isatidis medicinal materials can improve the content of effective components such as adenosine, uridine, guanosine and (R.S)-emetine in radix isatidis medicinal materials, so that high-quality medicinal materials can be applied to downstream production, the quality of finished products such as radix isatidis granules and radix isatidis oral liquid can be improved, and the curative effect of products on the market can be ensured.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a fresh handling method of radix isatidis. BACKGROUND

[0002] There are two methods for handling the fresh radix isatidis harvested: one is that the harvested radix isatidis is cleaned, packed, and then transported to a pre-treatment workshop, and then washed, sliced, dried, and packaged to obtain dried radix isatidis or decoction pieces, which are used for the preparation of radix isatidis granules, radix isatidis oral liquid and the like; the other is that the fresh radix isatidis is handled, and the fresh radix isatidis is washed, sliced, juiced, and extracted to obtain an extract, the extract is concentrated, and then spray-dried or freeze-dried, and then pulverized, sieved, and vacuum-packed to obtain powder decoction pieces. The first method is the most commonly used method, but the volume of the handled decoction pieces is large, the hygiene standard is poor, the decoction pieces are prone to be damp, moldy and insect-infested, and the storage and transportation costs are high; if the effective component content of the handled radix isatidis is unqualified or the effective component content is degraded during storage, the subsequent use of the radix isatidis may be unqualified, and the product demand cannot be met; the second method obtains powder decoction pieces through a complex process, and dried radix isatidis cannot be obtained, thereby limiting the application. SUMMARY

[0003] The application aims to provide a fresh handling method of radix isatidis.

[0004] According to the fresh handling method of radix isatidis, the method comprises the following steps:

[0005] (1) storing the fresh radix isatidis at 0-10℃ for 3-14 days;

[0006] (2) vacuum-drying the radix isatidis stored in step (1) at 50-70℃.

[0007] According to the fresh handling method of radix isatidis, in step (1), the fresh radix isatidis is stored at 0-10℃ for 3 days.

[0008] According to the fresh handling method of radix isatidis, in step (1), the fresh radix isatidis is stored at 0-10℃ for 7 days.

[0009] According to the fresh handling method of radix isatidis, in step (1), the fresh radix isatidis is stored at 0-10℃ for 14 days.

[0010] According to the fresh processing method of the radix isatidis medicinal material in the specific embodiment of the present application, in step (2), the refrigerated radix isatidis is vacuum dried at 50 DEG C.

[0011] According to the fresh processing method of the radix isatidis medicinal material in the specific embodiment of the present application, in step (2), the refrigerated radix isatidis is vacuum dried at 60 DEG C.

[0012] According to the fresh processing method of the radix isatidis medicinal material in the specific embodiment of the present application, in step (2), the refrigerated radix isatidis is vacuum dried at 70 DEG C.

[0013] According to the fresh processing method of the radix isatidis medicinal material in the specific embodiment of the present application, the vacuum dried radix isatidis is packaged and stored.

[0014] According to the fresh processing method of the radix isatidis medicinal material in the specific embodiment of the present application, the fresh radix isatidis is washed and cleaned of mud and sand, and then refrigerated at 0-10 DEG C.

[0015] According to the fresh processing method of the radix isatidis medicinal material in the specific embodiment of the present application, after vacuum drying, the content of adenosine in the radix isatidis medicinal material is greater than 0.6 mg / g.

[0016] The beneficial effects of the present application are:

[0017] The fresh processing method of the radix isatidis medicinal material of the present application can improve the content of effective components such as adenosine, uridine, guanosine, (R.S)-chaulmoogryl and the like in the radix isatidis medicinal material, so that high-quality medicinal materials are applied to downstream production, the quality of finished products such as radix isatidis granules and radix isatidis oral liquid is improved, and the curative effect of products on the market is ensured. Specific embodiments

[0018] To make the objectives, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described in detail below. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0019] Embodiment 1

[0020] 1.1 A fresh processing method of radix isatidis medicinal material, comprising the following steps:

[0021] (1) refrigerating fresh radix isatidis at 0 DEG C for 3 days;

[0022] (2) vacuum drying the refrigerated radix isatidis of step (1) at 50 DEG C, and packaging and storing.

[0023] 1.2 A method for fresh treatment of radix isatidis, comprising the following steps:

[0024] (1) Fresh radix isatidis is stored at 5℃ for 7 days;

[0025] (2) The radix isatidis stored in step (1) is vacuum dried at 60℃ and stored after packaging.

[0026] 1.3 A method for fresh treatment of radix isatidis, comprising the following steps:

[0027] (1) Fresh radix isatidis is stored at 10℃ for 14 days;

[0028] (2) The radix isatidis stored in step (1) is vacuum dried at 70℃ and stored after packaging.

[0029] Example 2

[0030] 2.1 Effect of different treatment methods on adenosine content in radix isatidis

[0031] After the radix isatidis is treated by different processes (the specific treatment methods are shown in Table 1), the powder is obtained by sieving through a No. 3 sieve, and the adenosine content of the powder is determined according to the following steps. The adenosine determination conditions are as follows:

[0032] ① Chromatographic conditions:

[0033] Chromatographic column: octadecylsilane bonded silica gel column; detection wavelength: 260 nm; column temperature: 30℃, sample size: 2uL; mobile phase: phosphate buffer (pH = 6.5)-methanol = 17:3; flow rate: 0.5mL / min, elution time: 1min.

[0034] ② Preparation of reference solution:

[0035] An appropriate amount of adenosine reference substance is accurately weighed, and a solution containing 20ug per 1mL is prepared by adding the mobile phase, and shaken to obtain the reference solution.

[0036] Each group of radix isatidis powder is divided into two parts, accurately weighed, placed in a conical flask, 30% methanol extractant is added, tightly sealed, shaken, weighed, cooled, weighed, and the weight loss is made up with the extractant, shaken, filtered, and the filtrate is obtained. A total of 18 groups of test samples are obtained.

[0037] ④ Determination: 2uL of the reference solution and the test sample solution is accurately taken and injected into the ultra-high performance liquid chromatograph for determination.

[0038] Table 1 Treatment method of radix isatidis and adenosine content obtained

[0039]

[0040]

[0041] From the data in Table 1, it can be seen that fresh Radix Isatidis can maintain enzyme activity, and in a low-temperature refrigeration environment, the enzyme can promote the increase of secondary metabolites and the increase of effective components. Fresh harvested Radix Isatidis is stored at 0-10℃ for seven days, and then vacuum dried at 50-70℃ after refrigeration. The adenosine content of the Radix Isatidis is increased by 40-50% compared with that of directly dried Radix Isatidis, and the adenosine content of the Radix Isatidis treated by freezing is decreased compared with that of directly dried Radix Isatidis.

[0042] From the data in the first, second and third groups, it can be seen that after the dried Radix Isatidis is re-wetted, the enzyme has been inactivated, and the adenosine content does not increase after refrigeration, indicating that the process of vacuum drying of fresh Radix Isatidis after refrigeration is an irreversible process.

[0043] 2.2 Determination of the contents of adenosine, uridine, guanosine and (R.S)-choline in the treated Radix Isatidis

[0044] After the fresh Radix Isatidis is treated by different processes (the specific treatment methods are shown in Table 2), the powder is sieved through a No. 3 sieve, and the obtained four groups of Radix Isatidis powders are determined according to the following steps:

[0045] ① Chromatographic conditions:

[0046] Chromatographic column: octadecylsilane bonded silica gel column; detection wavelength: 254 nm; column temperature: 30℃; mobile phase: A phase-methanol, B phase-water; flow rate: 0.8 mL / min.

[0047] ② Preparation of the control solution:

[0048] The uridine control, guanosine control, adenosine control and (R.S)-choline control are respectively weighed accurately, and 5% methanol solution is added to prepare a mixed solution containing 20 μg of uridine, 20 μg of guanosine, 25 μg of adenosine and 20 μg of (R.S)-choline per 1 ml, and the mixed solution is obtained.

[0049] ③ Preparation of the test solution:

[0050] The Radix Isatidis powder is prepared to a suitable concentration, 1 mL of which is accurately taken and placed in a 10 mL volumetric flask, 5% methanol solution is added to dilute to the calibration mark, shaken uniformly, filtered, and the filtrate is obtained.

[0051] ④ Determination: 5 μL of the test solution and the control solution are respectively accurately taken, injected into the liquid chromatograph, and determined, and the results are obtained.

[0052] Table 2 Treatment methods of Radix Isatidis and determination results of the contents of components

[0053]

[0054] From the above data, the harvested fresh radix isatis drug in 0-10℃ storage for seven days, after refrigeration in 50-70℃ vacuum drying, compared with the radix isatis drug directly vacuum drying, uridine, guanosine, (R.S)-adelfungin, adenosine are increased. Whether the harvesting of largeleaf goldthread leaf has little effect on the effective components of radix isatis.

[0055] The present application research found that the dried medicinal materials, even after wetting, after refrigeration treatment, the effective components are not increased; after freezing, the effective components are not only not increased, but decreased. The uridine content of fresh radix isatis after refrigeration and drying is increased by more than 70%, the guanosine content is increased by more than 45%, the (R,S)-adelfungin content is increased by more than 50%, and the adenosine content is increased by more than 40%.

[0056] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited to this, any skilled person in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered in the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A method for fresh handling of the medicinal material of Radix Isatidis, characterized in that, The method steps are as follows: (1) store fresh Radix Isatidis at 0-10℃ for 3-14 days; (2) vacuum dry the Radix Isatidis stored in step (1) at 50-70℃; pack and store the vacuum dried Radix Isatidis.

2. The method according to claim 1, characterized in that, In step (1), store fresh Radix Isatidis at 0-10℃ for 14 days.

3. The method according to claim 1, characterized in that, In step (2), vacuum dry the stored Radix Isatidis at 50℃.

4. The method according to claim 1, characterized in that, In step (2), vacuum dry the stored Radix Isatidis at 60℃.

5. The method according to claim 1, characterized in that, In step (2), vacuum dry the stored Radix Isatidis at 70℃.

Images