Magnetic levitation-based extracorporeal coagulation function detection device and detection method thereof

Abstract

This invention relates to an in vitro coagulation function testing device and method based on magnetic levitation. The device comprises a coagulation function testing unit, a drive control unit, a signal acquisition unit, a magnetic levitation unit, and a heating device. The drive control unit drives and controls the coagulation function testing unit. The coagulation function testing unit detects the changes in blood viscosity and density during blood coagulation. The signal acquisition unit acquires the changes in blood viscosity and density, as well as the coagulation time, and sends this information to a host computer. The magnetic levitation unit keeps the coagulation function testing unit in a magnetically levitated state, and the heating device heats the blood being tested by the coagulation function testing unit. The coagulation function testing device features high sensitivity, real-time detection capability, small size, and low cost. Therefore, this invention has broad application prospects in fields such as bioengineering and medicine.

Description

Technical Field

[0001] This invention belongs to the field of coagulation function testing technology, specifically relating to an in vitro coagulation function testing device and its testing method based on magnetic levitation. Background Technology

[0002] In recent years, with the increase in cardiovascular patients, departments such as surgery and intensive care units have increasingly greater needs for rapid assessment of blood coagulation, as well as anticoagulant drug testing and dosage adjustment. Therefore, rapid, low-volume, and convenient bedside methods for rapid coagulation function testing have become a research hotspot. Currently, clinical coagulation testing mainly uses wet biochemical coagulation tests such as magnetic bead methods, optical methods, and piezoelectric methods. Wet biochemical coagulation tests generally involve blood and liquid reagents in a reaction vessel, and the tests only measure a few key single factors. However, the coagulation process requires the combined action of more than a dozen coagulation factors. Wet biochemical coagulation testing methods cannot directly reflect platelet function and coagulation disorders, and the instruments are large and not portable. Summary of the Invention

[0003] To address the problems existing in the prior art, this invention provides an in vitro coagulation function testing device and method based on magnetic levitation, solving the problems of large instrument size, high cost, and inability to monitor in real time in the prior art.

[0004] The technical solution adopted by this invention to solve the technical problem is as follows:

[0005] An in vitro coagulation function testing device based on magnetic levitation includes: a coagulation function testing unit, a drive control unit, a signal acquisition unit, a magnetic levitation unit, and a heating device. The drive control unit drives and controls the coagulation function testing unit. The coagulation function testing unit detects the changes in blood viscosity and density during blood coagulation. The signal acquisition unit acquires the changes in blood viscosity and density, as well as the blood coagulation time, and sends this information to a host computer. The magnetic levitation unit keeps the coagulation function testing unit in a magnetically levitated state, and the heating device heats the blood being tested by the coagulation function testing unit.

[0006] Preferably, the coagulation function detection unit includes: a disposable plastic probe sleeve, a mechanical probe, and a retractable sleeve; the magnetic levitation unit includes: a sensor base, a left sensing magnet, an induction coil, a permanent magnet, an upper driving magnet, a right sensing magnet, a driven body, an elastic support, and a lower driving magnet; the disposable plastic probe sleeve is fitted over the mechanical probe, and the bottom surfaces of the disposable plastic probe sleeve and the mechanical probe are on the same horizontal line, inserted below the liquid surface in the blood sample test cup; the blood sample test cup is wrapped with a heating device, and the upper surfaces of the disposable plastic probe sleeve and the mechanical probe are positioned on the retractable sleeve. The retractable sleeve and the driven body are integrated into a single structure. The left and right sensing magnets are respectively disposed opposite each other on both sides of the inner wall of the sensor base. The permanent magnet with the induction coil is wound and fixed within the space formed by the left and right sensing magnets. An upper driving magnet is fixed below the permanent magnet. A certain distance is provided between the upper driving magnet and the driven body. The left and right sensing magnets sit on the elastic support, which is a ring structure, with the inner ring cooperating with the retractable sleeve. The lower driving magnet is disposed on the lower end face of the elastic support.

[0007] Preferably, the sensor base consists of an upper sensor base and a lower sensor base, which are fixed by screws.

[0008] Preferably, the disposable plastic probe sleeve is made of a polymer material.

[0009] Preferably, the driven body is a metal disc, which is bonded to the telescopic sleeve with epoxy resin adhesive.

[0010] Preferably, the outer side of the inner ring of the elastic support is connected to the inner side of the outer ring by a support rod.

[0011] Preferably, the elastic support prevents the coagulation function detection unit from resonating.

[0012] Preferably, the device further includes an amplifier circuit; the amplifier circuit is disposed between the drive control unit and the magnetic levitation unit.

[0013] A detection method for an in vitro coagulation function testing device based on magnetic levitation, comprising the following steps:

[0014] Step 1: Label the blood sample cups to be tested, and attach the disposable plastic probe to the mechanical probe, ensuring that the lower end of the mechanical probe is at least below the surface of the blood sample; place the blood sample cups in the heating device and turn on the power.

[0015] Step 2: The host computer transmits the start detection command to the drive control unit; the drive control unit transmits the signal to the amplification section. After the sinusoidal signal is amplified by the amplification circuit, the strength of the magnetic field generated by the up and down driving magnets of the magnetic levitation unit changes regularly, causing the telescopic sleeve to vibrate periodically in the vertical direction; as thrombin and fibrin are formed in the blood sample, the vibration frequency of the mechanical probe changes; the signal acquisition unit will continuously monitor the output frequency of the coagulation function detection unit.

[0016] Step 3: After the blood coagulation process is completed, the mechanical probe response frequency collected by the signal acquisition unit will reach dynamic equilibrium, thus completing the coagulation process test.

[0017] Preferably, the temperature of the heating device is around 37°C when it is turned on.

[0018] The beneficial effects of this invention are as follows: Addressing the shortcomings of existing technologies, this invention provides an in vitro coagulation function testing device and method based on magnetic levitation. The coagulation function testing device features high sensitivity, real-time detection capability, small size, and low cost. Therefore, this invention has broad application prospects in fields such as bioengineering and medicine. Attached Figure Description

[0019] Figure 1 This invention presents a schematic diagram of the signal transmission of an in vitro coagulation function testing device based on magnetic levitation.

[0020] Figure 2 A schematic diagram of the coagulation function detection unit, magnetic levitation unit, and heating device of this invention.

[0021] Figure 3 A schematic diagram of the elastic support structure of this invention.

[0022] Figure 4 The flowchart of the in vitro coagulation function detection method based on magnetic levitation of this invention. Detailed Implementation

[0023] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.

[0024] like Figure 1As shown, an in vitro coagulation function testing device based on magnetic levitation includes: a coagulation function testing unit, a drive control unit, an amplifier circuit, a signal acquisition unit, a magnetic levitation unit, and a heating device 2. The drive control unit sends a sinusoidal signal for driving and control to the amplifier circuit, which amplifies the sinusoidal signal and transmits it to the coagulation function testing unit. The coagulation function testing unit detects the changes in blood viscosity and density during blood coagulation. The signal acquisition unit collects the changes in blood viscosity and density, as well as the coagulation time, and sends this information to a host computer. The magnetic levitation unit keeps the coagulation function testing unit in a magnetically levitated state, and the heating device 2 heats the blood being tested in the coagulation function testing unit.

[0025] like Figure 2As shown, the coagulation function testing unit includes: a disposable plastic probe sleeve 3, a mechanical probe 4, and a retractable sleeve 6; the magnetic levitation unit includes: a sensor base, a left induction magnet 9, an induction coil 10, a permanent magnet 11, an upper driving magnet 12, a right induction magnet 13, a driven body 14, an elastic support 16, and a lower driving magnet 17; the disposable plastic probe sleeve 3 is fitted over the mechanical probe 4, and the bottom surfaces of the disposable plastic probe sleeve 3 and the mechanical probe 4 are on the same horizontal line, inserted below the liquid surface in the blood sample cup 5; in this embodiment, the disposable plastic probe sleeve 3 is made of a polymer material, so it does not need to be replaced for each test and can be used multiple times. The blood sample cup 5 is wrapped with a heating device 2, and in this embodiment, the temperature of the heating device 2 is controlled at about 37 degrees Celsius. The upper parts of the disposable plastic probe sleeve 3 and the mechanical probe 4 are set on the telescopic sleeve 6, which is an integral structure with the driven body 14. In this embodiment, the driven body is a metal disc, which is bonded to the telescopic sleeve with epoxy resin. The left sensing magnet 9 and the right sensing magnet 13 are respectively arranged opposite to each other on both sides of the inner wall of the sensor base. The permanent magnet 11 is wound and fixed by the sensing coil 10 in the space formed by the left sensing magnet 9 and the right sensing magnet 13. The upper driving magnet 12 is fixed below the permanent magnet 11. There is a certain distance between the upper driving magnet 12 and the driven body 14. The left sensing magnet 9 and the right sensing magnet 13 sit on the elastic support 16, which is a ring structure, and the inner ring cooperates with the telescopic sleeve 6. The lower driving magnet 17 is set on the lower end face of the elastic support 16. When energized, the induction coil 10 generates an induced current by cutting the magnetic fields produced by the left induction magnet 9 and the right induction magnet 13, thus creating a magnetic field between the upper driving magnet 12 and the lower driving magnet 17. By changing the magnitude of the energizing current, the magnetic field between the upper driving magnet 12 and the lower driving magnet 17 is altered, changing the distance between the driven body 14 and the upper driving magnet 12, thereby causing the retractable sleeve 6 to drive the mechanical probe 4 to reciprocate up and down within the liquid surface. Figure 3 As shown, the outer side of the inner ring of the elastic support 16 is connected to the inner side of the outer ring by a support rod. An inner pressure ring 18 is provided between the inner ring and the telescopic sleeve 6 to ensure that the mechanical probe 4 will not resonate with the coagulation function detection unit during normal operation and will not produce horizontal displacement when it moves up and down in the liquid.

[0026] In this embodiment, the sensor base is divided into an upper sensor base 7 and a lower sensor base 8, which are fixed by screws 15. The heating unit 2 is covered by a device housing 1, which is made of aluminum material to ensure that the detection device has good anti-electromagnetic interference capability.

[0027] like Figure 4As shown, the detection method of the in vitro coagulation function testing device based on magnetic levitation includes the following steps:

[0028] Step 1: Label the blood sample cups 5 to be tested, and attach the disposable plastic probe sleeve 3 to the mechanical probe 4, ensuring that the lower end of the mechanical probe 4 is at least below the surface of the blood sample; place the blood sample cups 5 in the heating device 2 and turn on the power, setting the temperature to around 37 degrees Celsius.

[0029] Step 2: The host computer transmits the start detection command to the drive control unit; the drive control unit transmits the signal to the amplification circuit. After the sinusoidal signal is amplified by the amplification circuit, the strength of the magnetic field generated by the upper and lower drive magnets of the magnetic levitation unit will change regularly, changing the distance between the driven body 14 and the upper drive magnet 12, causing the retractable sleeve 6 fixed to the driven body 14 to vibrate periodically in the vertical direction; as thrombin and fibrin are formed in the blood sample, the vibration frequency of the mechanical probe 4 changes; the signal acquisition unit will continuously monitor the output frequency of the coagulation function detection unit.

[0030] Step 3: After the blood coagulation process is completed, the response frequency of the mechanical probe 4 collected by the signal acquisition unit will reach dynamic equilibrium and be sent to the host computer to complete the coagulation process test.

[0031] Step 4: After the test is completed, remove the blood sample cup 5 and the disposable plastic probe sleeve 3. Clean and dry the testing equipment, turn off the power to the device, and store the device properly.

Claims

1. An in vitro coagulation function testing device based on magnetic levitation, characterized in that, The device includes: a coagulation function detection unit, a drive control unit, a signal acquisition unit, a magnetic levitation unit, and a heating device (2); the drive control unit drives and controls the coagulation function detection unit; the coagulation function detection unit detects the changes in blood viscosity and density during blood coagulation; the signal acquisition unit acquires the changes in blood viscosity and density and the time of blood coagulation, and sends this information to the host computer; the magnetic levitation unit keeps the coagulation function detection unit in a magnetically levitated state; the heating device (2) heats the blood being tested by the coagulation function detection unit, and the temperature of the heating device is 37°C when it is turned on. The coagulation function detection unit includes: a disposable plastic probe sleeve (3), a mechanical probe (4), and a retractable sleeve (6); the magnetic levitation unit includes: a sensor base, a left induction magnet (9), an induction coil (10), a permanent magnet (11), an upper driving magnet (12), a right induction magnet (13), a slave (14), an elastic support (16), and a lower driving magnet (17); the disposable plastic probe sleeve (3) is fitted over the mechanical probe (4), and the bottom surfaces of the disposable plastic probe sleeve (3) and the mechanical probe (4) are on the same horizontal line, inserted below the liquid surface in the blood sample cup (5); the blood sample cup (5) is wrapped with a heating device (2), and the upper surfaces of the disposable plastic probe sleeve (3) and the mechanical probe (4) are set in the retractable sleeve (6), the retractable sleeve (6) and the slave The body (14) is an integrated structure. The driven body (14) is a metal disc, which is bonded to the telescopic sleeve (6) with epoxy resin. The left sensing magnet (9) and the right sensing magnet (13) are respectively arranged opposite to each other on both sides of the inner wall of the sensor base. The permanent magnet (11) is wound and fixed in the space formed by the left sensing magnet (9) and the right sensing magnet (13). The upper driving magnet (12) is fixed below the permanent magnet (11). There is a certain distance between the upper driving magnet (12) and the driven body (14). The left sensing magnet (9) and the right sensing magnet (13) sit on the elastic support (16). The elastic support (16) is a ring structure, and the inner ring cooperates with the telescopic sleeve (6). The lower driving magnet (17) is arranged on the lower end face of the elastic support (16).

2. The in vitro coagulation function testing device based on magnetic levitation according to claim 1, characterized in that, The sensor base is divided into an upper sensor base (7) and a lower sensor base (8), which are fixed by screws (15).

3. The in vitro coagulation function testing device based on magnetic levitation according to claim 1, characterized in that, The disposable plastic probe sleeve (3) is made of polymer material.

4. The in vitro coagulation function testing device based on magnetic levitation according to claim 1, characterized in that, The outer side of the inner ring of the elastic support (16) is connected to the inner side of the outer ring by a support rod.

5. The in vitro coagulation function testing device based on magnetic levitation according to claim 1, characterized in that, The elastic support (16) prevents the coagulation function detection unit from resonating.

6. The in vitro coagulation function testing device based on magnetic levitation according to claim 1, characterized in that, The device also includes an amplifier circuit; the amplifier circuit is disposed between the drive control unit and the magnetic levitation unit.

7. A detection method based on the magnetic levitation-based in vitro coagulation function testing device according to any one of claims 1-6, characterized in that, The method includes the following steps: Step 1: Label the blood sample cups (5) to be tested, and attach the disposable plastic probe sleeve (3) to the mechanical probe (4), ensuring that the lower end of the mechanical probe (4) is at least below the surface of the blood sample; place the blood sample cups (5) in the heating device (2) and turn on the power. Step 2: The start detection command is transmitted to the drive control unit via the host computer; the drive control unit transmits the signal to the amplification section. After the sine signal is amplified by the amplification circuit, the strength of the magnetic field generated by the up and down driving magnets of the magnetic levitation unit will change regularly, causing the telescopic sleeve (6) to vibrate periodically in the vertical direction. As thrombin and fibrin are formed in the blood sample, the vibration frequency of the mechanical probe (4) changes; the signal acquisition unit will continuously monitor the output frequency of the coagulation function detection unit. Step 3: After the blood coagulation process is completed, the response frequency of the mechanical probe (4) collected by the signal acquisition unit will reach dynamic equilibrium, thus completing the coagulation process test.

8. The detection method according to claim 7, characterized in that, The temperature of the heating device (2) is around 37°C when it is turned on.

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