A handheld adjustable SPR sensor device

Abstract

The invention relates to a handheld adjustable SPR sensor device, comprising a probe assembly, a shell, an incident assembly, a signal collection assembly and a main control chip respectively arranged in the shell, and a display screen arranged on the surface of the shell, wherein the probe assembly comprises a probe shell, a reflector and a metal film, the probe shell comprises a conical head and a columnar tail movably connected to each other, the columnar tail is arranged on the side of the shell, the reflector and the metal film are arranged on the inner side of the conical head and are perpendicular to each other to form an SPR sensor group, the incident assembly and the signal collection assembly are connected to the probe shell and are respectively opposite to the reflector and the metal film, and the main control chip is respectively connected to the signal collection assembly and the display screen. Compared with the prior art, the invention has the advantages of convenient use, wide application range and the like.

Description

Technical Field

[0001] The invention relates to the technical field of surface plasmon resonance sensors, and in particular to a handheld adjustable SPR sensor device. Background Art

[0002] Surface Plasma Resonance (SPR) technology has developed rapidly in recent years due to its high sensitivity and low cost. Surface Plasma Resonance (SPR) technology is a technology that detects the composition and concentration of a sample by measuring the change in the refractive index of the sample. The sensitive layer can be modified on the SPR sensor chip or the SPR sensor probe to make the sensor only sensitive to a certain substance, or the thickness of the metal film layer can be changed to control the refractive index measurement range.

[0003] SPR sensors are divided into angle modulation, wavelength modulation, intensity modulation and phase modulation. At present, most of the surface plasmon resonance measurement methods use wavelength modulation and angle modulation. The angle modulation method requires a very precise measuring device, which must have a precise stepper motor to drive the angle adjustment, and is generally large in size. If wavelength modulation is used, a spectrometer is required, which is relatively expensive. The phase modulation method measurement device will be very complicated. Compared with the above three measurement methods, the light intensity modulation test system has the advantages of simple structure and low cost, and does not use complex angle control devices and spectrometers, which makes it easy to miniaturize the instrument.

[0004] The fiber optic SPR probe invented by patent application publication number CN102252999A can only detect a single range of substances. If other items need to be measured, another set of equipment needs to be replaced, and the measured data needs to be observed on a computer, which is not convenient for specific scenarios such as field testing. Therefore, there is an urgent need for a handheld sensor that integrates an SPR sensor and a detection device into one device to form an integrated detection and testing device. Summary of the invention

[0005] The purpose of the present invention is to provide a handheld adjustable SPR sensor device in order to overcome the defects of the above-mentioned prior art. The SPR sensor probe group part is connected to the tip of the device by a rotatable device, and the SPR sensing surface is changed by rotation, so that items with different refractive index ranges can be measured in a low-cost manner. The sensor has a built-in STM32 single-chip microcomputer, and the data measured by the detector is directly displayed on a liquid crystal display screen for easy observation.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] A handheld adjustable SPR sensor device comprises a probe assembly, a shell, an incident assembly, a signal collection assembly and a main control chip respectively arranged in the shell, and a display screen arranged on the surface of the shell, wherein the probe assembly comprises a probe shell, a reflector and a metal film, the probe shell comprises a conical head and a columnar tail movably connected to each other, the columnar tail is arranged on the side of the shell, the reflector and the metal film are arranged on the inner side of the conical head and are perpendicular to each other to form an SPR sensor group, the incident assembly and the signal collection assembly are connected to the probe shell and face the reflector and the metal film respectively, and the main control chip is connected to the signal collection assembly and the display screen respectively;

[0008] When in use, the probe component of the device is set in the environmental medium to be detected, and the incident component injects polarized laser light directly into the reflector. The polarized laser light is reflected by the reflector to the metal film that can excite SPR. When the SPR excitation condition is met, the light intensity is changed, and the metal film produces surface plasma resonance and reflects the light to the signal acquisition component. The signal acquisition component obtains the light reflected by the metal film and sends it to the main control chip for calculation. Finally, the calculated refractive index of the environmental medium is displayed on the display screen.

[0009] Furthermore, the reflectors and metal films are provided in plurality, and are arranged on the inner side surfaces of the conical head in pairs, and are perpendicular to each other to form multiple groups of SPR sensor groups. The conical head can rotate along the axis of the columnar tail to rotate different SPR sensor groups to a position directly opposite the incident component and the signal acquisition component.

[0010] Furthermore, in the plurality of SPR sensing groups, the refractive index range of each group of metal thin films is different from each other;

[0011] When in use, the conical head is rotated as needed to switch the SPR sensing groups with different refractive index ranges.

[0012] Furthermore, the conical head comprises a conical tip and a cylindrical connecting portion which are connected to each other, the columnar tail is a cylindrical tail, and the cylindrical connecting portion and the cylindrical tail are connected to each other.

[0013] Furthermore, an annular groove is provided on the side of the cylindrical connecting portion, and an annular protrusion is provided on the side of the cylindrical tail portion. The annular protrusion is clamped in the annular groove to realize the rotation of the conical head.

[0014] Furthermore, the polarized laser incident from the incident component is parallel to the side wall of the columnar tail portion, and the angle between the incident component and the reflector is 45°.

[0015] Furthermore, the device also includes a power supply and a switch, and the power supply and the switch are respectively connected to the main control chip.

[0016] Furthermore, the incident component includes a polarized laser light source, an incident optical fiber and an incident optical fiber chuck, the incident optical fiber chuck is arranged at the columnar tail directly opposite the reflector, the polarized laser light source is connected to the main control chip, and the incident optical fiber is respectively connected to the polarized laser light source and the incident optical fiber chuck.

[0017] Furthermore, the signal acquisition component includes a reflective fiber optic chuck, a reflective fiber, a detector and a filter amplifier circuit connected in sequence, the reflective fiber optic chuck is arranged at the columnar tail facing the metal film, and the filter amplifier circuit is interconnected with the main control chip.

[0018] Preferably, the main control chip is an STM32 main control chip, and the display screen is a liquid crystal display screen.

[0019] Compared with the prior art, the present invention has the following advantages:

[0020] 1) The present invention changes the traditional detection mode of the SPR sensor, designs the probe sensing group and the detection group to be integrated, forms a handheld SPR sensor, puts the device into the environmental medium to be tested, and directly observes the data on the liquid crystal display screen of the sensor device, without connecting to a computer for data display, so that it can be out of the laboratory and portable;

[0021] 2) The present invention can change the metal film with different refractive index required for SPR sensing by rotating the conical head, and select the coating and modification under different conditions by rotating the face with the assistance of external force, so as to form different probe groups, without considering the problem of optical fiber coupling caused by replacing the metal film of the SPR sensing part, and by designing the mutually nested rotating structure of the annular groove and the annular protrusion, the SPR sensing group can be replaced without a sophisticated and complex rotating device, which is easy to operate and can perform diversified real-time measurements, and can measure the concentrations of various pollutants in sewage and air in real time;

[0022] 3) The present invention uses intensity modulation, the whole device does not require expensive instruments, the cost is low, the preparation process is simple, and mass production can be achieved. At the same time, the sensor has a simple structure, can be miniaturized, and the detection process is stable;

[0023] 4) The sensor of the present invention has a built-in battery, which can be used outside the laboratory and applied to field measurement or actual industrial environments by charging. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the internal structure of the patent of this invention;

[0025] Figure 2 This is a bottom view of the conical head of the present invention;

[0026] Figure 3 This is a schematic diagram of the tip rotation structure of the SPR sensor probe of the present invention;

[0027] Figure 4 This is the overall structural diagram of the patent of this invention.

[0028] Among them, 1. polarized laser light source, 2. incident optical fiber, 3. incident optical fiber chuck, 4. reflector, 5. metal film, 6. reflecting optical fiber chuck, 7. reflecting optical fiber, 8. detector, 9. filter amplifier circuit, 10. main control chip, 11. switch, 12. power supply, 13. display screen, 14. shell. DETAILED DESCRIPTION

[0029] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work should belong to the scope of protection of the present invention.

[0030] Example

[0031] like Figure 1-Figure 4 As shown, the present invention discloses a handheld adjustable SPR sensor device, which can be divided into an incident unit, a sensing unit and a data acquisition unit, specifically including a probe assembly, a shell 14, an incident assembly respectively arranged in the shell 14, a signal acquisition assembly and a main control chip 10, and a display screen 13 arranged on the surface of the shell 14. The probe assembly includes a probe shell, a reflector 4 and a metal film 5. The probe shell includes a conical head and a columnar tail that are movably connected to each other. The columnar tail is arranged on the side of the shell 14. The reflector 4 and the metal film 5 are arranged on the inner side of the conical head and are perpendicular to each other to form an SPR sensing group. The incident assembly and the signal acquisition assembly are connected to the probe shell and are respectively opposite to the reflector 4 and the metal film 5. The main control chip 10 is respectively connected to the signal acquisition assembly and the display screen 13.

[0032] like Figure 2 As shown, a plurality of reflectors 4 and metal films 5 are provided, and are arranged on the inner side of the conical head in pairs, and are perpendicular to each other to form multiple groups of SPR sensor groups, and the conical head can rotate along the axis of the columnar tail, and is used to rotate different SPR sensor groups to the position directly opposite the incident component and the signal acquisition component, and in the multiple groups of SPR sensor groups, the refractive index range of each group of metal films 5 is different from each other. When in use, the conical head can be rotated as needed to switch the SPR sensor groups with different refractive index ranges. In this embodiment, a total of four groups of SPR sensor groups are provided, and the angles of each reflector 4 and metal film 5 are 45°.

[0033] like Figure 3 As shown, the conical head includes a conical tip and a cylindrical connecting portion connected to each other, the columnar tail is a cylindrical tail, the cylindrical connecting portion and the cylindrical tail are connected to each other, an annular groove is provided on the side of the cylindrical connecting portion, and an annular protrusion is provided on the side of the cylindrical tail, which is clamped in the annular groove to realize the rotation of the conical head.

[0034] The device also includes a power supply 12 and a switch 11, and the power supply 12 and the switch 11 are respectively connected to the main control chip 10. The incident component includes a polarized laser light source 1, an incident optical fiber 2 and an incident optical fiber chuck 3, the incident optical fiber chuck 3 is arranged at the columnar tail facing the reflector 4, the polarized laser light source 1 is connected to the main control chip 10, and the incident optical fiber 2 is respectively connected to the polarized laser light source 1 and the incident optical fiber chuck 3. The signal acquisition component includes a reflection optical fiber chuck 6, a reflection optical fiber 7, a detector 8 and a filter amplifier circuit 9 connected in sequence, the reflection optical fiber chuck 6 is arranged at the columnar tail facing the metal film 5, and the filter amplifier circuit 9 is interconnected with the main control chip 10. Among them, the main control chip 10 is an STM32 main control chip, the display screen 13 is a liquid crystal display screen, and the power supply 12 is a rechargeable power supply.

[0035] The handheld replaceable SPR sensor probe assembly has a conical structure located at the tip of the device, with two opposite inclined surfaces forming a group, and the two surfaces of each SPR sensor group being perpendicular to each other, one of the surfaces being provided with a reflector 4 for use as a reflective surface, and the opposite surface being plated with metals of different thicknesses or having different sensitive layers modified on the metal as SPR reaction layers for realizing detection of the substance to be tested.

[0036] The patented handheld replaceable SPR sensor of the present invention is used together with a polarized laser light source 1 and a test system, and the measurement is achieved in the following way: ensure that the propagation direction of the incident light source of the SPR sensor is parallel to the side wall of the probe group device. Each SPR sensing surface maintains a 90° angle with the reflective surface, and the incident light source is incident on the tip of the probe. The light beam is reflected by the reflective surface and flipped 90° to the SPR sensing surface to form plasma resonance. The detector detects the data of the output light intensity affected by the change of the refractive index, which is controlled by the STM32 single-chip microcomputer and displayed on the built-in LCD screen. The front end of the probe has a rotating device, which can realize the detection of different items by rotating and replacing the coating or modification of different conditions of the SPR sensing area.

[0037] When in use, the probe component of the device is set in the environmental medium to be detected, and the incident component directly injects polarized laser light into the reflector 4. The polarized laser light injected by the incident component is parallel to the side wall of the cylindrical tail, and the angle between it and the reflector 4 is 45°. The polarized laser light is reflected by the reflector 4 to the metal film 5 that can excite SPR. When the SPR excitation condition is met, the light intensity is changed, the metal film 5 produces surface plasmon resonance phenomenon, and reflects the light to the signal acquisition component. The signal acquisition component obtains the light reflected by the metal film 5 and sends it to the main control chip 10 for calculation, and finally the calculated refractive index of the environmental medium is displayed on the display screen 13.

[0038] When the probe is inserted into the liquid to be tested, the light emitted by the polarized laser light source 1 is incident on the SPR probe of the pyramid structure by the optical fiber chuck 3 and reflected by the reflector 4 to the metal film 5 that can excite SPR. When the SPR excitation condition is met, the reflected light intensity is changed and enters the photoelectric conversion circuit by another optical fiber chuck 6 after reflection. The photoelectric conversion circuit is composed of a coaxial detector and a filter amplifier circuit. After data acquisition, the signal is controlled by the STM32 main control chip 10 and displayed on the liquid crystal display 13. The device is equipped with a rechargeable power supply 12 and a switch 11. The device is inserted into the guessed liquid. After the switch is turned on, the STM32 main control chip 10 controls the laser and the photoelectric conversion circuit to provide power, and the SPR sensor is started to detect.

[0039] In the present invention, the role of the metal film 5 in the SPR sensor probe is to produce surface plasma resonance. When the incident light is transmitted from the optical fiber to the metal film 5, a certain wavelength of light will resonate with the plasma wave on the surface of the metal-environmental medium, and the light of this specific wavelength will be absorbed. When the light is reflected and analyzed by the detector and computer, a resonance absorption peak can be observed at this wavelength. The position of this specific wavelength depends on the refractive index of the environmental medium. This is the measurement principle of the SPR sensor. The thickness, length, coating quality and modifiers of the metal film 5 are important factors in determining the quality of the sensor. Figure 3 As shown, the SPR probe of the pyramid part of the SPR sensor has a rotatable front end, and every 45 degree rotation can switch a metal film 5 with a refractive index range to form an SRP sensing group.

[0040] In this embodiment, the front rotating structure and the side wall of the SPR sensor probe are nested with each other, and the calibers match each other, so as to achieve good nesting between the two sections, thereby eliminating the cumbersome caliber docking. The SPR sensor probe can be mass-produced, and the measurement is convenient without affecting the sensitivity and normal use of the sensor; the probe is easy to replace, and different probe groups can be formed by rotating the surface with the assistance of external force to select coating and modification under different conditions, so as to carry out diversified real-time measurement, and can implement real-time measurement of the concentration of various pollutants in sewage and air.

[0041] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person familiar with the technical field can easily think of various equivalent modifications or substitutions within the technical scope disclosed by the present invention, and these modifications or substitutions should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. A handheld adjustable SPR sensor device, characterized in that: The invention comprises a probe assembly, a shell (14), an incident assembly, a signal collection assembly and a main control chip (10) respectively arranged in the shell (14), and a display screen (13) arranged on the surface of the shell (14); the probe assembly comprises a probe shell, a reflector (4) and a metal film (5); the probe shell comprises a conical head and a columnar tail which are movably connected to each other; the columnar tail is arranged on the side of the shell (14); the reflector (4) and the metal film (5) are arranged on the inner side of the conical head and are perpendicular to each other to form an SPR sensor group; the incident assembly and the signal collection assembly are connected to the probe shell and are respectively opposite to the reflector (4) and the metal film (5); and the main control chip (10) is respectively connected to the signal collection assembly and the display screen (13); When in use, the probe assembly of the device is placed in an environmental medium to be detected, the incident assembly directly faces the reflector (4) to inject polarized laser light, the polarized laser light is reflected by the reflector (4) to the metal film (5) capable of stimulating SPR, when the SPR excitation condition is met, the light intensity is changed, the metal film (5) generates a surface plasma resonance phenomenon, and reflects the light to the signal acquisition assembly, the signal acquisition assembly acquires the light reflected by the metal film (5) and sends it to the main control chip (10) for calculation, and finally displays the calculated refractive index of the environmental medium through the display screen (13); The reflectors (4) and metal films (5) are provided in plurality, and are arranged on the inner side of the conical head in pairs, and are perpendicular to each other to form a plurality of SPR sensor groups. The conical head can rotate along the axis of the columnar tail to rotate different SPR sensor groups to a position directly facing the incident component and the signal collection component. In the plurality of SPR sensing groups, the refractive index range of each group of metal thin films (5) is different from each other; When in use, the conical head is rotated as needed to switch the SPR sensor groups with different refractive index ranges; The conical head comprises a conical tip and a cylindrical connecting portion which are connected to each other, the columnar tail is a cylindrical tail, and the cylindrical connecting portion and the cylindrical tail are connected to each other.

2. A handheld adjustable SPR sensor device according to claim 1, characterized in that: An annular groove is provided on the side of the cylindrical connecting portion, and an annular protrusion is provided on the side of the cylindrical tail portion. The annular protrusion is clamped in the annular groove to realize the rotation of the conical head.

3. A handheld adjustable SPR sensor device according to claim 1, characterized in that: The polarized laser light incident from the incident component is parallel to the side wall of the columnar tail portion, and the angle between the incident laser light and the reflector (4) is 45 degrees.

4. A handheld adjustable SPR sensor device according to claim 1, characterized in that: The device also includes a power supply (12) and a switch (11), wherein the power supply (12) and the switch (11) are respectively connected to the main control chip (10).

5. A handheld adjustable SPR sensor device according to claim 1, characterized in that: The incident component comprises a polarized laser light source (1), an incident optical fiber (2) and an incident optical fiber chuck (3); the incident optical fiber chuck (3) is arranged at a position of the columnar tail directly facing the reflector (4); the polarized laser light source (1) is connected to a main control chip (10); and the incident optical fiber (2) is respectively connected to the polarized laser light source (1) and the incident optical fiber chuck (3).

6. A handheld adjustable SPR sensor device according to claim 1, characterized in that: The signal acquisition component comprises a reflective optical fiber chuck (6), a reflective optical fiber (7), a detector (8) and a filter amplifier circuit (9) which are connected in sequence. The reflective optical fiber chuck (6) is arranged at the columnar tail directly facing the metal film (5), and the filter amplifier circuit (9) is interconnected with a main control chip (10).

7. A handheld adjustable SPR sensor device according to claim 1, characterized in that: The main control chip (10) is an STM32 main control chip, and the display screen (13) is a liquid crystal display screen.

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