Medical hematopoietic stem cell collection instrument

Abstract

The present invention belongs to the technical field of medical equipment, and specifically is a medical hematopoietic stem cell collector, comprising a blood collection needle and a blood collection needle tube; a liquid outlet is provided at the bottom of the blood collection needle tube; a negative pressure mechanism is connected to the inner sliding seal of the blood collection needle tube; a baffle located on the left side of the negative pressure mechanism is installed on the inner wall of the blood collection needle tube; a sleeve is sleeved on the outer wall of the blood collection needle tube, a through hole is provided on the top of the sleeve, and the sleeve can rotate with the blood collection needle tube as the axis; a liquid tank is fixedly installed on the right side of the blood collection needle, and the blood collection needle and the liquid tank are connected; the push plate is driven to move vertically downward by the lifting mechanism, and the heparin liquid on the top of the push plate will also move downward, thereby reducing the squeezing of the negative pressure mechanism by the heparin liquid, and the negative pressure mechanism moves to the right side in the blood collection needle tube, so that the negative pressure inside the blood collection needle tube draws blood into the blood collection needle tube through the blood collection needle, thereby achieving the effect of controlling the total amount of blood collected.

Description

Technical Field

[0001] The invention belongs to the technical field of medical equipment, in particular to a medical hematopoietic stem cell collection instrument. Background Art

[0002] Hematopoietic stem cell collectors use a blood collection needle to pierce the skin, and then use the negative pressure inside the blood collection needle tube to extract the blood and stem cells in the blood. Hematopoietic stem cell collectors are an indispensable part of bone marrow transplantation.

[0003] For example, a Chinese patent with application number CN201810179181.7 discloses a medical hematopoietic stem cell collector, including a mounting body, a blood collection unit, a stem cell separation unit, a stem cell collection tank, a plasma collection tank, a pressure pump, a negative pressure pump, a heparin bottle, a plasma delivery tube and an ice storage box, wherein the blood collection unit is connected to the stem cell separation unit; the stem cell separation unit is connected to the stem cell collection tank; the stem cell separation unit is also connected to the plasma separation tank; the pressure pump is connected to the plasma collection tank; the negative pressure pump is connected to the stem cell collection tank; the heparin bottle is connected to the stem cell separation unit; one end of the plasma delivery tube is connected to the blood collection unit, and the other end of the plasma delivery tube is connected to the plasma collection tank; a physiological saline introduction tube is also provided on the plasma delivery tube; the present invention can improve the purification accuracy during stem cell collection while maintaining a continuous working state.

[0004] However, the prior art uses a negative pressure pump to provide negative pressure to extract blood from the human body. The amount of blood drawn by negative pressure is difficult to control, and a large amount of blood accumulates in the chamber. The hematopoietic stem cells in the blood will be squeezed, causing damage to the cell wall and resulting in cell inactivation, which affects the extraction of hematopoietic stem cells. When the negative pressure is generated by manually kneading the rubber tube to draw blood, although the amount of blood drawn can be controlled, the negative pressure is variable. When the negative pressure is extremely high, the hematopoietic stem cells in the drawn blood will be squeezed and ruptured due to the pressure, causing cell inactivation. At the same time, if the blood after negative pressure extraction is not in contact with the heparin solution in time, it will be very easy to coagulate due to the coagulation factors in the blood. The coagulated blood will squeeze the hematopoietic stem cells in the blood due to the change in volume, causing cell inactivation.

[0005] Therefore, we proposed a medical hematopoietic stem cell collection instrument to solve the above problems. Summary of the invention

[0006] In order to make up for the deficiencies of the prior art, the present invention proposes a medical hematopoietic stem cell collector to solve the problem that the prior art uses a negative pressure pump to provide negative pressure to extract blood from the human body, the amount of negative pressure blood extraction is difficult to control, and a large amount of blood accumulates in the cavity, the hematopoietic stem cells in the blood will be squeezed, causing damage to the cell wall and leading to cell inactivation, affecting the extraction of hematopoietic stem cells, and when blood is drawn by manually kneading a rubber hose to generate negative pressure, although the amount of blood drawn can be controlled, the negative pressure is variable. When the negative pressure is extremely large, the hematopoietic stem cells in the extracted blood will be squeezed and ruptured due to the pressure, causing cell inactivation.

[0007] The technical solution adopted by the present invention to solve the technical problem is as follows: the medical hematopoietic stem cell collector of the present invention comprises a blood collection needle and a blood collection needle tube; a liquid outlet is provided on the side wall of the blood collection needle tube; a piston mechanism is connected to the inner sliding seal of the blood collection needle tube; a sleeve is sleeved at the liquid outlet of the side wall of the blood collection needle tube, and a through hole is provided on the top of the sleeve;

[0008] The blood collection needle tube is fixed with a connected liquid box at one end of the piston mechanism, and the other end of the blood collection needle tube is movably connected with a blood collection needle; the internal sliding sealing connection of the liquid box is connected with a push plate; a lifting mechanism is fixedly installed between the push plate and the bottom of the liquid box; the side wall of the liquid box is fixedly connected with a liquid outlet pipe.

[0009] Preferably, a liquid inlet pipe is fixedly connected to the side wall of the liquid tank, and a solenoid valve is fixedly installed inside the liquid inlet pipe.

[0010] Preferably, the piston mechanism comprises a floating plate and a spring; the floating plate is fixedly connected to the liquid tank via the spring.

[0011] Preferably, the ends of the floating plate and the liquid outlet are both provided with drainage arc chamfers.

[0012] Preferably, the lifting mechanism comprises a motor, a crank and a push rod; the motor is fixedly mounted on the side wall of the liquid tank; the crank is fixedly mounted on the motor shaft; and both ends of the push rod are hinged on the crank and the push plate, respectively.

[0013] Preferably, the lifting mechanism further comprises a support plate and a deceleration rod; the support plate is fixedly mounted on the inner wall of the liquid tank; and both ends of the deceleration rod are fixedly mounted on the support plate and the push plate respectively.

[0014] Preferably, a connected drainage tube is fixed to the outer side of the liquid outlet; a blood storage box is fixedly installed at the bottom of the drainage tube, and a connected liquid outlet tube is fixed to the side wall of the blood storage box.

[0015] Preferably, the top of the drainage tube is trumpet-shaped, and the inner diameter of the drainage tube decreases from the top to the bottom.

[0016] Preferably, the blood storage box is internally slidably and sealingly connected with a support plate; an elastic rod is fixedly installed between the support plate and the bottom of the blood storage box.

[0017] Preferably, the blood collection needle head and the blood collection needle tube are fastened and connected via threaded engagement.

[0018] The beneficial effects of the present invention are as follows:

[0019] 1. The medical hematopoietic stem cell collector described in the present invention drives the push plate to move vertically downward through the lifting mechanism, and the heparin liquid on the top of the push plate also moves downward, thereby reducing the squeezing of the piston mechanism by the heparin liquid. The piston mechanism moves to the right in the blood collection needle tube, so that the negative pressure inside the blood collection needle tube draws blood into the blood collection needle tube through the blood collection needle, thereby achieving the effect of controlling the total amount of blood collected.

[0020] 2. In the medical hematopoietic stem cell collector described in the present invention, after the push plate moves to the bottom of the liquid outlet tube, the liquid outlet tube discharges the heparin liquid, thereby moving the piston mechanism. Since the amount of heparin liquid discharged is controlled by the diameter of the liquid outlet tube, the speed at which the blood collection needle draws blood from the human body can also be controlled, thereby achieving the effect of controlling the blood collection speed. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention will be further described below in conjunction with the accompanying drawings.

[0022] Figure 1 is a cross-sectional view of the present invention;

[0023] Figure 2 It is a three-dimensional diagram of the liquid tank;

[0024] Figure 3 It is a schematic diagram of a blood collection needle tube;

[0025] Figure 4 This is a comparison of the two devices when drawing blood for 10 minutes;

[0026] Figure 5 This is a comparison of the two devices when drawing blood for 10 minutes;

[0027] In the figure: 1. blood collection needle; 2. blood collection needle tube; 21. liquid outlet; 22. piston mechanism; 221. floating plate; 222. spring; 23. stop rod; 24. sleeve; 241. through hole; 3. liquid tank; 31. liquid inlet pipe; 311. solenoid valve; 32. push plate; 33. lifting mechanism; 331. motor; 332. crank; 333. push rod; 334. support plate; 335. deceleration rod; 34. liquid outlet; 4. drainage tube; 5. blood storage box; 51. support plate; 52. elastic rod. DETAILED DESCRIPTION

[0028] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0029] like Figures 1 to 5 As shown, a medical hematopoietic stem cell collector of the present invention comprises a blood collection needle 1 and a blood collection needle tube 2; a liquid outlet 21 is provided on the side wall of the blood collection needle tube 2; a piston mechanism 22 is connected to the inner sliding seal of the blood collection needle tube 2; a sleeve 24 is sleeved at the liquid outlet 21 of the side wall of the blood collection needle tube 2, and a through hole 241 is provided on the top of the sleeve 24;

[0030] The blood collection needle tube 2 is fixed with a connected liquid box 3 at one end of the piston mechanism 22, and the other end of the blood collection needle tube 2 is movably connected with the blood collection needle 1; the inner sliding sealing connection of the liquid box 3 is connected with a push plate 32; a lifting mechanism 33 is fixedly installed between the push plate 32 and the bottom of the liquid box 3; the side wall of the liquid box 3 is fixedly connected with a liquid outlet pipe 34;

[0031] In the prior art, a negative pressure pump is used to provide negative pressure to extract blood from a human body. The amount of blood extracted by negative pressure is difficult to control, and a large amount of blood accumulates in the chamber. Hematopoietic stem cells in the blood will be squeezed, causing damage to the cell wall and resulting in cell inactivation, which affects the extraction of hematopoietic stem cells. When blood is extracted by manually kneading a rubber hose to generate negative pressure, although the amount of blood drawn can be controlled, the negative pressure is variable. When the negative pressure is extremely high, the hematopoietic stem cells in the extracted blood will be squeezed and ruptured due to the pressure, resulting in cell inactivation.

[0032] The present invention drives the push plate 32 to move vertically downward through the lifting mechanism 33. When the push plate 32 moves downward, the heparin liquid between the top of the push plate 32 and the top of the liquid tank 3 moves downward, and the hematopoietic stem cells can survive longer in the heparin liquid. When the push plate 32 moves to the position of the liquid outlet pipe 34, the heparin liquid level drops to the top position of the blood collection needle tube 2, but the heparin liquid still fills the right cavity of the piston mechanism 22 in the blood collection needle tube 2. Subsequently, when the push plate 32 moves to the position of the liquid outlet pipe 34, the heparin liquid level drops to the top position of the blood collection needle tube 2, but the heparin liquid still fills the right cavity of the piston mechanism 22 in the blood collection needle tube 2. Continuing to move downward, the push plate 32 will fall below the liquid outlet pipe 34, and the heparin liquid will be discharged along the liquid outlet pipe 34, and will mix with the blood discharged from the blood collection needle tube 2 after extraction, thereby increasing the survival time of hematopoietic stem cells in the blood, and reducing the heparin liquid content between the top of the push plate 32 and the top of the liquid tank 3, so that the pressure exerted by the heparin liquid inside the blood collection needle tube 2 on the piston mechanism 22 will be reduced, so that the piston mechanism 22 moves toward the liquid tank 3, so that the cavity inside the blood collection needle tube 2 on the left side of the piston mechanism 22 will increase, so that under the action of negative pressure, the blood collection needle 1 pierced into the human skin will draw the blood in the human body into the blood collection needle tube 2. Since the height of the liquid level is slowly lowered by the lifting mechanism 33, the amount of heparin liquid discharged from the liquid tank 3 is controlled by the inner diameter of the liquid outlet pipe 34. Since the inner diameter of the liquid outlet pipe 34 remains unchanged, the rate at which the heparin liquid flows out of the liquid outlet pipe 34 is constant, so the piston mechanism 22 The speed of movement inside the blood collection needle tube 2 is constant, so the pressure of the blood collected by the blood collection needle tube 2 is constant, and there will be no sudden change in pressure that causes a change in blood flow rate, causing the stem cells in the blood to touch each other and causing cell wall rupture. At the same time, since the internal cavity of the blood collection needle tube 2 is constant, the content of the extracted blood can be controlled. Compared with the prior art that uses a negative pressure pump to drive a blood extraction tube to extract blood, the amount of blood extracted is more accurate, avoiding excessive blood extraction to harm human health, and a large amount of blood accumulation will increase the probability of contact and collision of stem cells in the blood, causing damage to the stem cells. Subsequently, the sleeve 24 is rotated so that the through hole 241 on the sleeve 24 is opposite to the liquid outlet 21. At this time, the blood inside the blood collection needle tube 2 can be released. Initially, the through hole 241 on the sleeve 24 and the liquid outlet 21 are misaligned, so that the blood collection needle tube 2 is in a sealed state, avoiding pressure leakage of the blood collection needle tube 2 and causing the problem of failure of negative pressure blood collection.

[0033] As an embodiment of the present invention, the side wall of the liquid tank 3 is fixedly connected with a liquid inlet pipe 31, and a solenoid valve 311 is fixedly installed inside the liquid inlet pipe 31; when working, after the heparin liquid is discharged from the liquid outlet pipe 34, the piston mechanism 22 moves, and negative pressure is generated inside the blood collection needle tube 2 to extract blood. After the blood is discharged from the blood collection needle tube 2, the piston mechanism 22 needs to be reset. At this time, the lifting mechanism 33 is required to send the push plate 32 back to the initial position, and then the solenoid valve 311 inside the liquid inlet pipe 31 is opened, and then the liquid is infused into the liquid tank 3 through the liquid inlet pipe 31. With the help of the increase in the amount of heparin liquid, the piston mechanism 22 returns to the initial position, thereby achieving the effect of resetting the piston mechanism 22 and the blood collection needle tube 2 can be used again after cleaning.

[0034] As an embodiment of the present invention, the piston mechanism 22 includes a float plate 221 and a spring 222; the float plate 221 is fixedly connected to the liquid tank 3 by the spring 222, and the density of the float plate 221 is less than that of the heparin liquid; when working, the density of the float plate 221 is less than that of the heparin liquid. When the heparin liquid pushes the float plate 221 to move, the force generated on the float plate 221 is divided into buoyancy and liquid pressure, which is beneficial for the heparin liquid to push the float plate 221 to move toward the left, so that negative pressure is generated inside the blood collection needle tube 2, and the provision of the spring 222 is beneficial for the float plate 221 to move to the right inside the blood collection needle tube 2, so that the volume of the left cavity of the blood collection needle tube 2 increases to generate negative pressure to extract blood.

[0035] As an embodiment of the present invention, the floating plate 221 and the end of the liquid outlet 21 are both provided with drainage arc chamfers; during operation, when the through hole 241 on the sleeve 24 and the liquid outlet 21 on the blood collection needle tube 2 are aligned, the blood in the blood collection needle tube 2 will be discharged, and the floating plate 221 and the end of the liquid outlet 21 are both provided with drainage arc chamfers, which can drain the blood in the blood collection needle tube 2, avoid blood retention in the blood collection needle tube 2, and improve the utilization rate of collected blood.

[0036] As an embodiment of the present invention, the lifting mechanism 33 includes a motor 331, a crank 332 and a push rod 333; the motor 331 is fixedly mounted on the side wall of the liquid tank 3; the crank 332 is fixedly mounted on the shaft of the motor 331; the two ends of the push rod 333 are respectively hinged on the crank 332 and the push plate 32; when working, the crank 332 is driven to rotate by the motor 331, and the push rod 333 will move together with the crank 332 during the rotation process. When the push rod 333 moves downward with the crank 332, the push rod 333 no longer generates a supporting force on the push plate 32, and the push plate 32 will move vertically downward under the weight of the heparin liquid, so that when the push plate 32 moves to the bottom of the liquid outlet pipe 34, the heparin liquid is discharged, and then, after the crank 332 drives the push rod 333 to reset, the push plate 32 will push the heparin liquid back to the initial position, thereby realizing the effect of automatic control of the push plate 32.

[0037] As an embodiment of the present invention, the lifting mechanism 33 also includes a support plate 334 and a deceleration rod 335; the support plate 334 is fixedly mounted on the inner wall of the liquid tank 3; the two ends of the deceleration rod 335 are respectively fixedly mounted on the support plate 334 and the push plate 32; when working, by installing the deceleration rod 335 at the bottom of the push plate 32, after the push rod 333 does not generate a supporting force on the push plate 32, the deceleration rod 335 generates a deceleration effect on the movement of the push plate 32, thereby avoiding that the heparin liquid initially generates a large impact force after the push plate 32 drops suddenly, so that a large amount of heparin liquid is initially discharged from the liquid outlet pipe 34, resulting in a large negative pressure in the blood collection needle tube 2 at the initial time, resulting in a large blood extraction rate of the blood collection needle 1 at the initial time, which is not conducive to the survival of hematopoietic stem cells in the blood, thereby achieving the effect of improving the survival rate of hematopoietic stem cells in the blood.

[0038] As an embodiment of the present invention, a connected drainage tube 4 is fixed to the outer side of the liquid outlet 21; a blood storage box 5 is fixedly installed at the bottom of the drainage tube 4, and a connected liquid outlet tube 34 is fixed to the side wall of the blood storage box 5; when working, the blood in the blood collection needle tube 2 is drained into the blood storage box 5 through the drainage tube 4. Since the side wall of the blood storage box 5 is connected to the liquid outlet tube 34, before the blood flows into the blood storage box 5, the blood storage box 5 already contains heparin solution. Subsequently, after the blood flows into the blood storage box 5, the heparin solution has an anti-coagulation effect on the blood to prevent blood coagulation.

[0039] As an embodiment of the present invention, the top of the drainage tube 4 is trumpet-shaped, and the inner diameter of the drainage tube 4 decreases from the top to the bottom; when working, the trumpet-shaped drainage tube 4 can effectively collect the blood flowing out of the blood collection needle tube 2, and the trumpet-shaped drainage tube 4 can drain the blood at the top. At the same time, the inner diameter of the drainage tube 4 continuously decreases from high to low, which can slow down the blood flowing out from the top of the drainage tube 4, and prevent the blood from falling into the liquid tank 3 at an excessively fast speed, causing hematopoietic stem cells in the blood to expand and be damaged, thereby achieving the effect of improving blood collection effect and improving the survival of hematopoietic stem cells in the blood.

[0040] As an embodiment of the present invention, the blood storage box 5 is internally slidingly sealed and connected with a support plate 51; an elastic rod 52 is fixedly installed between the support plate 51 and the bottom of the blood storage box 5; during operation, by installing the support plate 51 inside the blood storage box 5 and installing the elastic rod 52 at the bottom of the support plate 51, after blood flows into the support plate 51, causing the weight above the support plate 51 to change, the elastic rod 52 at the bottom of the support plate 51 will drive the support plate 51 to vibrate, so that the blood can be mixed with the heparin solution, avoiding the coagulation of the blood on the upper layer of the heparin solution due to not contacting the heparin solution, thereby achieving the effect of improving the anti-coagulation properties of the blood and increasing the survival rate of hematopoietic stem cells in the blood.

[0041] As an embodiment of the present invention, the blood collection needle 1 is fastened to the blood collection needle tube 2 by threaded engagement; when working, the blood collection needle 1 needs to pierce the human skin. After one blood collection, the blood collection needle 1 pierced into the human skin cannot be used a second time, which will cause cross infection. Therefore, the blood collection needle 1 needs to be replaced so that the blood collection needle tube 2 can be used multiple times to avoid waste of the blood collection needle tube 2. Therefore, the inner cavity of the blood collection needle 1 is provided with an internal thread, and the outer wall of the blood collection needle tube 2 is provided with an external thread. The blood collection needle 1 and the blood collection needle tube 2 are fastened by threads, so that the blood collection needle 1 can be replaced in time after use. Since the blood collection needle tube 2 is connected to the liquid tank 3, it is inconvenient to replace it frequently. By replacing the blood collection needle 1 and timely disinfecting the inside of the blood collection needle tube 2, cross infection can be effectively avoided.

[0042] The specific workflow is as follows:

[0043] The lifting mechanism 33 drives the push plate 32 to move vertically downward. When the push plate 32 moves downward, the heparin liquid between the top of the push plate 32 and the top of the liquid tank 3 moves downward, and the hematopoietic stem cells can survive longer in the heparin liquid. When the push plate 32 moves to the position of the liquid outlet pipe 34, the heparin liquid level drops to the top position of the blood collection needle tube 2, but the heparin liquid still fills the right side cavity of the piston mechanism 22 in the blood collection needle tube 2. Subsequently, when the push plate 32 continues to move downward, the push plate 32 will fall below the liquid outlet pipe 34, and the heparin liquid will be discharged along the liquid outlet pipe 34, and will be mixed with the blood discharged from the blood collection needle tube 2 after extraction, thereby increasing the survival time of the hematopoietic stem cells in the blood, and reducing the heparin liquid content between the top of the push plate 32 and the top of the liquid tank 3, so that the pressure generated by the heparin liquid in the blood collection needle tube 2 on the piston mechanism 22 will be reduced, so that the piston mechanism 22 moves toward the liquid tank 3, so that the blood collection needle tube 2 on the left side of the piston mechanism 22 The internal cavity will increase, so that under the action of negative pressure, the blood collection needle 1 inserted into the human skin will draw the blood in the human body into the blood collection needle tube 2. Since the lifting mechanism 33 lowers the liquid level slowly, the amount of heparin liquid discharged from the liquid tank 3 is controlled by the inner diameter of the liquid outlet tube 34. Since the inner diameter of the liquid outlet tube 34 remains unchanged, the rate at which the heparin liquid flows out through the liquid outlet tube 34 is constant. Therefore, the speed at which the piston mechanism 22 moves inside the blood collection needle tube 2 is constant. Therefore, the pressure of the blood collected by the blood collection needle tube 2 is constant, and there will be no sudden change in pressure causing a change in the blood flow rate, causing the stem cells in the blood to touch and cause cell wall rupture. At the same time, since the internal cavity of the blood collection needle tube 2 is constant, the content of the extracted blood can be controlled. Compared with the prior art that uses a negative pressure pump to drive a blood collection tube to extract blood, the amount of blood extracted is more accurate, avoiding excessive blood extraction to injure people. The body is healthy, and a large amount of blood is drawn and accumulated, which will increase the probability of contact and collision of stem cells in the blood, causing damage to the stem cells. Subsequently, the sleeve 24 is rotated so that the through hole 241 on the sleeve 24 is opposite to the liquid outlet 21. At this time, the blood inside the blood collection needle tube 2 can be released. Initially, the through hole 241 on the sleeve 24 and the liquid outlet 21 are misaligned, so that the blood collection needle tube 2 is in a sealed state, avoiding pressure leakage of the blood collection needle tube 2, resulting in the problem of failure of negative pressure blood collection; the density of the float plate 221 is less than that of the heparin liquid. When the heparin liquid pushes the float plate 221 to move, the force generated on the float plate 221 is divided into buoyancy and liquid pressure, which is conducive to the heparin liquid pushing the float plate 221 to move to the left, so that negative pressure is generated inside the blood collection needle tube 2, and the spring 222 is arranged to facilitate the float plate 221 to move to the right inside the blood collection needle tube 2, so that the volume of the left cavity of the blood collection needle tube 2 is increased to generate negative pressure to extract blood.

[0044] Experiments verify that the survival rate of hematopoietic stem cells in the blood extracted by the present invention is higher than that of hematopoietic stem cells in the blood extracted by the prior art using a negative pressure pump. In a sterile laboratory, blood samples with contents of 10 ml and 50 ml are respectively extracted by using the prior art and placed in test tubes. At the same time, blood samples with contents of 10 ml and 50 ml are respectively extracted by using the above-mentioned technical scheme and placed in test tubes. The mouth of the test tube is sealed with aluminum foil, and the aluminum foil is opened to add a 5 mg / ml MTT solution into the test tube, wherein 5 mg / ml MTT is obtained by weighing 5 mg of MTT solid and dissolving it in 100 ml of PBS, wherein PBS is a phosphate buffer. At the same time, the dissolved solution needs to be filtered using a 0.22 ul filter membrane to ensure that the solution is sterile, and then the solution is treated at 4°C in the dark.

[0045] After the MTT solution is added to the test tube, the test tube is sealed again. The respiratory chain in the mitochondria of the surviving cells in the test tube will react with the MTT solution. Under the action of succinate dehydrogenase and cytochrome C in the active cells, the exogenous MTT is reduced to water-insoluble blue-purple crystalline formazan and deposited in the cells. Then open the aluminum foil and add DMSO to the test tube. The blue-purple crystalline formazan will dissolve in DMSO. Since the reaction between DMSO and blue-purple crystalline formazan will produce substances harmful to the human body, at this time, the test tube needs to be quickly sealed with aluminum foil after adding DMSO. Subsequently, the light absorption value is measured at a wavelength of 570nm using an enzyme-linked immunosorbent assay, and the light absorption value can reflect the number of surviving cells.

[0046]

[0047] It can be concluded from the above experimental data that the above box table is the average of the 10ml and 50ml measurement data. Figure 4 Figure 5 The line graph shows that the prior art and the above technical solution collect fifteen sets of data for 10ml and 50ml respectively. Figure 4 Figure 5 The solid line in the middle is the liquid tank extraction, the dotted line is the negative pressure pump extraction, and the y-axis represents the light absorption value, in %. The line graph shows that when the blood content is 10 ml, the survival rate of hematopoietic stem cells obtained by the existing technology and the above-mentioned technical solution is not much different, but the data will be more stable during the blood collection process using the above-mentioned technical solution, that is, the cell survival rate is more stable; after the blood content is 50 ml, the survival rate of hematopoietic stem cells obtained by the above-mentioned technical solution is significantly improved compared with the existing technology, and it can be obtained that when the existing technology is used to extract hematopoietic stem cells, as the blood concentration increases, the cells will be damaged. The experimental data can prove that the above-mentioned technical solution has significant progress compared with the existing technology.

[0048] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A medical hematopoietic stem cell collection instrument, comprising a blood collection needle (1) and a blood collection needle tube (2); characterized in that: The side wall of the blood collection needle tube (2) is provided with a liquid outlet (21); the inside of the blood collection needle tube (2) is slidably sealed and connected to a piston mechanism (22); a sleeve (24) is sleeved at the liquid outlet (21) of the side wall of the blood collection needle tube (2), and a through hole (241) is provided at the top of the sleeve (24); a liquid box (3) is fixedly connected to one end of the piston mechanism (22) of the blood collection needle tube (2), and a blood collection needle (1) is movably connected to the other end of the blood collection needle tube (2); a push plate (32) is slidably sealed and connected to the inside of the liquid box (3); a lifting mechanism (33) is fixedly installed between the push plate (32) and the bottom of the liquid box (3); the side wall of the liquid box (3) is fixedly connected to a liquid outlet pipe (34); A liquid inlet pipe (31) is fixedly connected to the side wall of the liquid tank (3), and a solenoid valve (311) is fixedly installed inside the liquid inlet pipe (31); The piston mechanism (22) comprises a floating plate (221) and a spring (222); the floating plate (221) is fixedly connected to the liquid tank (3) via the spring (222); The ends of the floating plate (221) and the liquid outlet (21) are both provided with drainage arc chamfers; The lifting mechanism (33) comprises a motor (331), a crank (332) and a push rod (333); the motor (331) is fixedly mounted on the side wall of the liquid tank (3); the crank (332) is fixedly mounted on the shaft of the motor (331); the two ends of the push rod (333) are respectively hinged on the crank (332) and the push plate (32); The lifting mechanism (33) further comprises a support plate (334) and a deceleration rod (335); the support plate (334) is fixedly mounted on the inner wall of the liquid tank (3); the two ends of the deceleration rod (335) are respectively fixedly mounted on the support plate (334) and the push plate (32); A connected drainage tube (4) is fixed to the outside of the liquid outlet (21); a blood storage box (5) is fixedly installed at the bottom of the drainage tube (4), and a connected liquid outlet tube (34) is fixed to the side wall of the blood storage box (5); The top of the drainage tube (4) is trumpet-shaped, and the inner diameter of the drainage tube (4) decreases from the top to the bottom; The blood storage box (5) is internally slidably sealed and connected to a support plate (51); an elastic rod (52) is fixedly installed between the support plate (51) and the bottom of the blood storage box (5); The push plate (32) is driven to move vertically downward by the lifting mechanism (33). When the push plate (32) moves downward, the heparin liquid between the top of the push plate (32) and the top of the liquid tank (3) moves downward, and the hematopoietic stem cells can survive longer in the heparin liquid. When the push plate (32) moves to the position of the liquid outlet tube (34), the level of the heparin liquid drops to the top of the blood collection needle tube (2), but the heparin liquid still fills the piston mechanism in the blood collection needle tube (2). (22), and then, when the push plate (32) continues to move downward, the push plate (32) will fall below the liquid outlet tube (34), and the heparin liquid will be discharged along the liquid outlet tube (34), and will be mixed with the blood discharged from the blood collection needle tube (2) after extraction, thereby increasing the survival time of hematopoietic stem cells in the blood, and reducing the heparin liquid content between the top of the push plate (32) and the top of the liquid tank (3), so that the heparin liquid inside the blood collection needle tube (2) has an effect on the piston mechanism (22). The generated pressure will decrease, so that the piston mechanism (22) moves toward the liquid box (3), so that the cavity inside the blood collection needle tube (2) on the left side of the piston mechanism (22) will increase, so that under the action of negative pressure, the blood collection needle (1) inserted into the human skin will draw blood in the human body into the blood collection needle tube (2). Since the height of the liquid level is slowly lowered by the lifting mechanism (33), the amount of heparin liquid discharged from the liquid box (3) is controlled by the inner diameter of the liquid outlet tube (34). Since the inner diameter of the liquid outlet tube (34) is unchanged, the rate at which the heparin liquid flows out through the liquid outlet tube (34) is constant, so the speed at which the piston mechanism (22) moves inside the blood collection needle tube (2) is constant, so the pressure of the blood collected by the blood collection needle tube (2) is constant, and there will be no sudden change in pressure that causes a change in blood flow rate, causing the stem cells in the blood to touch and cause the cell wall to rupture. At the same time, since the internal cavity of the blood collection needle tube (2) is constant, the content of the extracted blood can be controlled.

2. A medical hematopoietic stem cell collection instrument according to claim 1, characterized in that: The blood collection needle (1) and the blood collection needle tube (2) are fastened and connected via threaded engagement.

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