A disinfecting composition, disinfecting solution, use and method of preparation

Abstract

The present application relates to the technical field of indoor disinfection, and particularly relates to a disinfection composition, application of the disinfection composition, and a disinfection liquid prepared from the disinfection composition and a preparation method of the disinfection liquid. The disinfection composition comprises the following components in parts by weight: forsythia suspensa extract 0.1-1 parts, honeysuckle extract 0.1-1 parts, didecyldimethylammonium chloride 0.05-0.15 parts, polyaminopropyl biguanide 0.1-0.5 parts, and benzalkonium chloride 0.05-0.5 parts. The disinfection composition has excellent bactericidal effect, and is mild, non-irritating to the skin, and non-corrosive to metal, and can be prepared into a disinfection liquid for daily disinfection at home.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of indoor disinfection, in particular to a disinfectant composition, application thereof and a disinfectant solution prepared from the disinfectant composition and a preparation method thereof. BACKGROUND

[0002] Biological contamination in indoor environment seriously threatens people's health and can cause various diseases, such as various respiratory infectious diseases, asthma, building syndrome, etc. Bacteria and fungi (including fungal spores) are indoor biological contaminants, which generally exist on the surface of objects frequently contacted in daily life, such as furniture, sanitary wares, kitchen utensils, cleaning tools, floors, walls, etc., and can multiply in large quantities in a warm and humid environment. For example, the kitchen sink is humid and often accumulates garbage, and pathogenic bacteria can grow in the hidden gaps; the faucet and its surrounding area are often wet due to running water, which is an ideal place for bacteria to multiply. When bacteria adhere to the faucet or sink through hands or food, they can multiply in large quantities in the faucet or sink, and eventually form a microbial film. When the microbial film breaks as it grows larger, bacteria can contaminate food and tableware, endangering human health. For another example, the door mat often contacts shoe soles and accumulates a large amount of E. coli and other pathogenic bacteria, which can be brought into the indoor environment every time the door mat is stepped on. For another example, if the washing machine is not cleaned and dried in time after each use, it is easy to accumulate dirt and breed bacteria, and at the same time, it can cause secondary pollution to clothes. In addition, bacteria can also be suspended in the air in the form of aerosol due to air flow, causing indoor air biological contamination. Therefore, people in the indoor environment contaminated by bacteria can be harmed by bacteria through contact or respiration. According to research, even if ultraviolet irradiation is used, there will still be a small amount of bacteria remaining in the indoor environment.

[0003] At present, disinfectant solutions commonly used in home environment include 75% medical alcohol, 84 disinfectant solution, and peracetic acid. However, 75% medical alcohol can enter bacteria, but cannot coagulate proteins in the bacteria, so it cannot kill the bacteria completely, and the alcohol has a strong and irritating smell, which is not suitable for large-area household disinfection, and the alcohol is flammable, which has safety hazards during storage; 84 disinfectant solution has a stimulating smell and strong corrosive effect, which can burn the respiratory mucosa and cause discomfort to people in the indoor environment, and the 84 disinfectant solution has bleaching and corrosive properties, which can cause discoloration of furniture, metal products and textiles, and is not suitable for disinfection of colored fabrics or metal products; peracetic acid is a strong oxidizing agent, which is extremely unstable, flammable and explosive, and has strong corrosive properties, and has certain safety hazards during storage and use, and can cause damage and corrosion to the skin, colored fabrics and metal products.

[0004] In addition, the surfactants, builders and other chemical additives added in the commercially available disinfectants can destroy the oily protective layer on the skin surface, thus aggravating the corrosion and damage of the disinfectants to the skin, and frequent contact can cause dry, rough and even cracked skin, and also cause different degrees of damage to the hair and other organs of the human body. SUMMARY

[0005] In view of the problems of corrosion and skin irritation of the current indoor disinfectants, the present application provides a disinfectant composition, a disinfectant solution, an application and a preparation method.

[0006] To achieve the above-mentioned purposes, the embodiments of the present application adopt the following technical solutions:

[0007] In one aspect, the present application provides a disinfectant composition comprising the following components in parts by weight: 0.1-1 parts of forsythia suspensa extract, 0.1-1 parts of honeysuckle extract, 0.05-0.15 parts of didecyldimethylammonium chloride, 0.1-0.5 parts of polyaminopropyl biguanide and 0.05-0.5 parts of benzalkonium chloride.

[0008] In the disinfectant composition, benzalkonium chloride has a wide bactericidal spectrum and strong bactericidal activity, and has little irritation to the skin and tissues, and no corrosive effect on metals and rubber products. Benzalkonium chloride changes the permeability of bacterial cytoplasmic membrane, causes the exosmosis of bacterial cytoplasmic substances and thus blocks their metabolism, thereby playing a role in killing bacteria. Polyaminopropyl biguanide interacts with groups or elements in organisms to destroy their normal material and energy metabolism, and is easily adsorbed by bacteria and viruses to inhibit the division function of bacteria and viruses. The combination of the two can enhance their effects on bacterial division and metabolism, thereby more quickly and effectively killing bacteria and inhibiting bacterial reproduction.

[0009] However, benzalkonium chloride binds to proteins quickly, so its bactericidal effect will decrease when it encounters cotton, cellulose and other organic matter. Didecyldimethylammonium chloride has excellent surface activity and wetting properties, and can still exert high-efficiency bactericidal effect in the presence of organic dirt, and help maintain the bactericidal activity of benzalkonium chloride, so that the composition has high bactericidal efficiency and long-term bacteriostatic effect. Didecyldimethylammonium chloride itself is also a broad-spectrum and safe bactericidal substance, which has excellent biological killing effect on gram-positive and negative bacteria, molds, viruses and algae, as well as envelope viruses such as hepatitis B and HIV, so the combination of didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride can achieve excellent disinfection effect.

[0010] The Forsythia extract has strong inhibiting effect on Staphylococcus aureus and Shigella dysenteriae, and also has inhibiting effect on Salmonella typhi, Vibrio cholerae, Escherichia coli, Pseudomonas aeruginosa, Bordetella pertussis, Mycobacterium tuberculosis, Diplococcus pneumoniae, Streptococcus haemolyticus and influenza virus, and has antifungal effect. The honeysuckle extract has certain inhibiting effect on various pathogenic bacteria such as Staphylococcus aureus, Streptococcus haemolyticus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae, Salmonella typhi, Salmonella paratyphi, etc., has inhibiting and killing effect on Diplococcus pneumoniae, Streptococcus meningitidis, Pseudomonas aeruginosa, Mycobacterium tuberculosis, Shigella dysenteriae and Streptococcus mutans, and can inhibit influenza virus, orphan virus, herpes virus and Leptospira. It is found in the research that the Forsythia extract and the honeysuckle extract have the effects of repairing skin, improving cell activity and maintaining normal state of elastic fiber, collagen fiber and protein polysaccharide, and the disinfecting composition obtained by compounding the two with didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride in the above dosage range not only has excellent disinfecting and bacteriostatic effects, but also can significantly reduce the irritation and damage of benzalkonium chloride, polyaminopropyl biguanide and didecyldimethylammonium chloride to skin, and the disinfecting composition is milder to skin than benzalkonium chloride, polyaminopropyl biguanide and didecyldimethylammonium chloride used alone. In addition, the disinfecting composition has no bleaching effect and other damage effect on furniture and textile, and has no corrosion to metal products.

[0011] Preferably, the disinfecting composition comprises the following ingredients in the weight percentage: 0.1-0.3 parts of the Forsythia extract, 0.1-0.3 parts of the honeysuckle extract, 0.05-0.1 parts of didecyldimethylammonium chloride, 0.3-0.5 parts of polyaminopropyl biguanide and 0.05-0.15 parts of benzalkonium chloride.

[0012] Preferably, the disinfecting composition comprises the following ingredients in the weight percentage: 0.1 part of the Forsythia extract, 0.1 part of the honeysuckle extract, 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride. The disinfecting composition with the above ratio has more excellent disinfecting effect, and has no irritation to skin and no damage to furniture and textile.

[0013] Optionally, the preparation method of the Forsythia extract comprises the following steps: heating and refluxing the Forsythia in 45-65% v / v aqueous ethanol solution adjusted to pH 8-8.5 by sodium hydroxide; filtering after the extraction is completed, concentrating the obtained filtrate, loading the concentrated filtrate on a polar macroporous adsorption resin, eluting the polar macroporous adsorption resin with 85-95% v / v aqueous ethanol solution, then eluting the polar macroporous adsorption resin with water, collecting water elution flow, and concentrating the collected water elution flow to 1.2-1.3 g / ml to obtain the Forsythia extract; wherein the mass of the 45-65% v / v aqueous ethanol solution is 5-8 times the mass of the Forsythia.

[0014] The disinfecting composition has excellent disinfecting effect, is mild, safe and non-irritating to skin, does not damage furniture and textile products, and does not corrode metal products, and can be prepared into household daily disinfecting products such as disinfecting spray, disinfecting wet wipe and disinfecting solution.

[0015] Optionally, the preparation method of the honeysuckle extract is as follows: honeysuckle is extracted by heating reflux with 55-60% v / v ethanol aqueous solution adjusted to pH 8-8.5 with sodium hydroxide, filtration is performed, the obtained residue is extracted by heating reflux with 25-35% v / v ethanol aqueous solution, filtration is performed, and the filtrates are combined and concentrated to 1.2-1.3 g / ml to obtain the honeysuckle extract.

[0016] The honeysuckle extract obtained by the above preparation method has better disinfecting effect and can reduce the irritation of the disinfecting composition, and the disinfecting effect and irritation reducing effect of the honeysuckle extract obtained by the above preparation method can be further improved when combined with the forsythia extract obtained by the above preparation method.

[0017] In a second aspect, the present application also provides use of the disinfecting composition in preparation of household daily disinfecting products.

[0018] The disinfecting composition has excellent disinfecting effect, is mild, safe and non-irritating to skin, does not damage furniture and textile products, and does not corrode metal products, and can be prepared into household daily disinfecting products such as disinfecting spray, disinfecting wet wipe and disinfecting solution.

[0019] In a third aspect, the present application also provides a disinfecting solution comprising the disinfecting composition. The disinfecting solution can be filled into a spray device as a spray for household daily disinfection, or can be directly used for wiping furniture and sanitary wares in a household environment, or for soaking and washing textile products such as floor mats, pillows and bed sheets.

[0020] The disinfecting solution comprises the following ingredients in parts by weight:

[0021]

[0022]

[0023] The humectant is at least one selected from propylene glycol, glycerol and butylene glycol;

[0024] The preservative is phenoxyethanol;

[0025] The solubilizer is PEG-40 hydrogenated castor oil;

[0026] The antioxidant is ethylenediaminetetraacetic acid disodium salt (disodium EDTA).

[0027] The above preferred raw materials are all commonly used components of compliant daily chemical products, and are highly safe, and will not cause obvious discomfort to the skin when used within the above dosage range. The citric acid can also enhance the disinfection performance of benzalkonium chloride, polyaminopropyl biguanide and didecyldimethylammonium chloride, and the citric acid itself also has certain bactericidal effect, so the disinfection effect of the disinfectant composition can be improved.

[0028] In a fourth aspect, the present application also provides a preparation method of the disinfectant solution, comprising the following steps:

[0029] The moisturizing agent is added to the water, and stirred until dissolved;

[0030] The didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride are added, and stirred until uniformly dispersed;

[0031] The forsythia suspensa extract, honeysuckle extract, citric acid, essence, solubilizer, antioxidant and preservative are added, and mixed until uniform, to obtain the disinfectant solution.

[0032] The temperature for adding the moisturizing agent is preferably 75-85°C, which can accelerate the dissolution of the moisturizing agent and improve the production efficiency.

[0033] The solution temperature when adding the didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride is preferably 48-52°C, which can accelerate the dissolution of the above components, and at the same time avoid decomposition of the above components or reaction between the above components to generate impurities at high temperature.

[0034] The solution temperature when adding the forsythia suspensa extract, honeysuckle extract, citric acid, essence, solubilizer, antioxidant and preservative is preferably 43-47°C, which can promote the forsythia suspensa extract and honeysuckle extract to be mixed with other components uniformly with the assistance of the solubilizer, and at the same time avoid decomposition of the citric acid, essence, antioxidant and preservative at higher temperature to lose effectiveness. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical scheme and advantages of the present application more clear, the present application will be further described in detail below in combination with specific examples. It should be understood that the specific examples described herein are only used to explain the present application, and are not used to limit the present application.

[0036] The experimental methods used in the following examples are all conventional methods in the art, unless otherwise specified.

[0037] The forsythia suspensa and honeysuckle used in the following examples all meet the relevant provisions of the respective medicinal materials in the text of Part One of the Chinese Pharmacopoeia (2015 edition).

[0038] The raw materials, reagents and the like used in the following examples are all obtained from commercial channels, unless otherwise specified.

[0039] Example 1

[0040] The disinfectant composition according to an embodiment of the present application comprises the following ingredients in the following proportions by weight:

[0041] 0.1 part of Forsythia suspensa extract, 0.1 part of Lonicera japonica extract, 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.

[0042] The Forsythia suspensa extract is prepared by heating and refluxing Forsythia suspensa with 55% v / v aqueous ethanol solution adjusted to pH 8.2 with sodium hydroxide, the mass of the 55% v / v aqueous ethanol solution being 7 times the mass of the Forsythia suspensa; after the extraction is completed, the filtrate is filtered, concentrated, loaded onto XDA-1 macroporous adsorption resin, eluted with 90% v / v aqueous ethanol solution, then eluted with water, the water elution fraction is collected and concentrated to 1.25 g / ml, and the Forsythia suspensa extract is obtained.

[0043] The Lonicera japonica extract is prepared by heating and refluxing Lonicera japonica with 57% v / v aqueous ethanol solution adjusted to pH 8.2 with sodium hydroxide, the mass of the 57% v / v aqueous ethanol solution being 7 times the mass of the Lonicera japonica; the residue obtained after filtration is heated and refluxed with 30% v / v aqueous ethanol solution, the mass of the 30% v / v aqueous ethanol solution being 7 times the mass of the Lonicera japonica; the filtrate obtained after filtration is combined with the filtrate obtained after the second extraction, and concentrated to 1.25 g / ml, and the Lonicera japonica extract is obtained.

[0044] Example 2

[0045] The disinfectant composition according to an embodiment of the present application comprises the following ingredients in the following proportions by weight:

[0046] 0.1 part of Forsythia suspensa extract, 0.1 part of Lonicera japonica extract, 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.

[0047] The Forsythia suspensa extract is prepared by heating and refluxing Forsythia suspensa with 45% v / v aqueous ethanol solution adjusted to pH 8 with sodium hydroxide, the mass of the 45% v / v aqueous ethanol solution being 5 times the mass of the Forsythia suspensa; after the extraction is completed, the filtrate is filtered, concentrated, loaded onto XDA-8 macroporous adsorption resin, eluted with 85% v / v aqueous ethanol solution, then eluted with water, the water elution fraction is collected and concentrated to 1.2 g / ml, and the Forsythia suspensa extract is obtained.

[0048] The preparation method of the honeysuckle extract is as follows: the honeysuckle is extracted by heating reflux with 55% v / v ethanol aqueous solution whose pH is adjusted to 8 by sodium hydroxide, and the mass of the 55% v / v ethanol aqueous solution is 6 times the mass of the honeysuckle; the obtained residue is filtered, and the residue is extracted by heating reflux with 25% v / v ethanol aqueous solution, and the mass of the 25% v / v ethanol aqueous solution is 6 times the mass of the honeysuckle; the filtrates obtained in the two extraction processes are combined, and concentrated to 1.2 g / ml, thereby obtaining the honeysuckle extract.

[0049] Example 3

[0050] The present application provides a disinfectant composition, which comprises the following components in the following proportions by weight:

[0051] 0.1 part of forsythia suspensa extract, 0.1 part of honeysuckle extract, 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.

[0052] The preparation method of the forsythia suspensa extract is as follows: the forsythia suspensa is extracted by heating reflux with 65% v / v ethanol aqueous solution whose pH is adjusted to 8.5 by sodium hydroxide, and the mass of the 65% v / v ethanol aqueous solution is 8 times the mass of the forsythia suspensa; after the extraction is completed, the obtained filtrate is filtered and concentrated, and then the concentrated filtrate is subjected to column chromatography with LSA-7 macroporous adsorption resin, eluted with 95% v / v ethanol aqueous solution, and then eluted with water; the water elution flow fraction is collected, and concentrated to 1.3 g / ml, thereby obtaining the forsythia suspensa extract.

[0053] The preparation method of the honeysuckle extract is as follows: the honeysuckle is extracted by heating reflux with 60% v / v ethanol aqueous solution whose pH is adjusted to 8.5 by sodium hydroxide, and the mass of the 60% v / v ethanol aqueous solution is 8 times the mass of the honeysuckle; the obtained residue is filtered, and the residue is extracted by heating reflux with 35% v / v ethanol aqueous solution, and the mass of the 35% v / v ethanol aqueous solution is 8 times the mass of the honeysuckle; the filtrates obtained in the two extraction processes are combined, and concentrated to 1.3 g / ml, thereby obtaining the honeysuckle extract.

[0054] Example 4

[0055] The present application provides a disinfectant composition, which comprises the following components in the following proportions by weight:

[0056] 0.3 part of forsythia suspensa extract, 0.3 part of honeysuckle extract, 0.05 part of didecyldimethylammonium chloride, 0.3 part of polyaminopropyl biguanide and 0.05 part of benzalkonium chloride.

[0057] The preparation method of the forsythia suspensa extract and the honeysuckle extract is the same as that in Example 1.

[0058] Example 5

[0059] The present application provides a disinfectant composition, which comprises the following components in the following proportions by weight:

[0060] Forsythia extract 0.3 parts, honeysuckle extract 0.3 parts, didecyldimethylammonium chloride 0.1 part, polyaminopropyl biguanide 0.4 part and benzalkonium chloride 0.05 part.

[0061] The preparation method of the Forsythia extract and the honeysuckle extract is the same as that in Example 1.

[0062] Example 6

[0063] The present application provides a disinfecting composition, which comprises the following ingredients in the following proportions by weight:

[0064] Forsythia extract 0.1 part, honeysuckle extract 0.1 part, didecyldimethylammonium chloride 0.05 part, polyaminopropyl biguanide 0.5 part and benzalkonium chloride 0.15 part.

[0065] The preparation method of the Forsythia extract and the honeysuckle extract is the same as that in Example 1.

[0066] Example 7

[0067] The present application provides a disinfecting composition, which comprises the following ingredients in the following proportions by weight:

[0068] Forsythia extract 0.1 part, honeysuckle extract 0.1 part, didecyldimethylammonium chloride 0.05 part, polyaminopropyl biguanide 0.1 part and benzalkonium chloride 0.05 part.

[0069] Example 8

[0070] The present application provides a disinfecting composition, which comprises the following ingredients in the following proportions by weight:

[0071] Forsythia extract 1 part, honeysuckle extract 1 part, didecyldimethylammonium chloride 0.15 part, polyaminopropyl biguanide 0.5 part and benzalkonium chloride 0.5 part.

[0072] Example 9

[0073] The present application provides a household daily disinfecting solution and a preparation method thereof, which comprises the following ingredients in the following proportions by weight:

[0074]

[0075] The preparation method of the Forsythia extract and the honeysuckle extract is the same as that in Example 1.

[0076] The preparation method of the disinfecting solution is as follows:

[0077] Add propylene glycol to water, and stir to completely dissolve at 80℃;

[0078] Cool down to 50℃, add didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride, stir and disperse evenly;

[0079] Cool down to 45℃, add forsythia suspensa extract and honeysuckle extract, as well as citric acid, essence, PEG-40 hydrogenated castor oil, disodium EDTA and phenoxyethanol;

[0080] Cool down to 40℃, and the disinfectant is obtained.

[0081] Example 10

[0082] The embodiment of the present application provides a kind of household routine disinfectant and preparation method thereof, and the disinfectant includes the following weight fraction ratio of component:

[0083]

[0084] Wherein the preparation method of forsythia suspensa extract and honeysuckle extract is same as embodiment 2.

[0085] The preparation method of the disinfectant is:

[0086] Add butylene glycol to water, and stir completely dissolved at 75℃;

[0087] Cool down to 48℃, add didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride, stir and disperse evenly;

[0088] Cool down to 43℃, add forsythia suspensa extract and honeysuckle extract, as well as citric acid, essence, PEG-40 hydrogenated castor oil, disodium EDTA and phenoxyethanol;

[0089] Cool down to 40℃, and the disinfectant is obtained.

[0090] Example 11

[0091] The embodiment of the present application provides a kind of household routine disinfectant and preparation method thereof, and the disinfectant includes the following weight fraction ratio of component:

[0092]

[0093]

[0094] Wherein the preparation method of forsythia suspensa extract and honeysuckle extract is same as embodiment 3.

[0095] The preparation method of the disinfectant is:

[0096] Add propylene glycol and glycerol to water, and stir completely dissolved at 85℃;

[0097] Cool down to 52℃, add didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride, stir and disperse evenly;

[0098] Cool to 47℃, add the extract of forsythia and honeysuckle, citric acid, essence, PEG-40 hydrogenated castor oil, disodium EDTA and phenoxyethanol;

[0099] Cool to 40℃, and the disinfectant is obtained.

[0100] Example 12

[0101] The present application provides a kind of household routine disinfectant and preparation method thereof, and the disinfectant includes the following weight fraction ratio of ingredients:

[0102]

[0103]

[0104] The preparation method of the extract of forsythia and honeysuckle is same as example 1.

[0105] The preparation method of the disinfectant is same as example 9.

[0106] Example 13

[0107] The present application provides a kind of household routine disinfectant and preparation method thereof, and the disinfectant includes the following weight fraction ratio of ingredients:

[0108]

[0109] The preparation method of the extract of forsythia and honeysuckle is same as example 1.

[0110] The preparation method of the disinfectant is same as example 9.

[0111] Example 14

[0112] The present application provides a kind of household routine disinfectant and preparation method thereof, and the disinfectant includes the following weight fraction ratio of ingredients:

[0113]

[0114] The preparation method of the extract of forsythia and honeysuckle is same as example 1.

[0115] The preparation method of the disinfectant is same as example 9.

[0116] Example 15

[0117] The present application provides a kind of household routine disinfectant and preparation method thereof, and the disinfectant includes the following weight fraction ratio of ingredients:

[0118]

[0119]

[0120] The preparation method of the extract of forsythia and honeysuckle is the same as that in Example 1.

[0121] The preparation method of the disinfectant is the same as that in Example 9.

[0122] Example 16

[0123] The present application provides a kind of household routine disinfectant and preparation method thereof, the disinfectant includes the following weight fraction ratio of component:

[0124]

[0125] The preparation method of the extract of forsythia and honeysuckle is the same as that in Example 1.

[0126] The preparation method of the disinfectant is the same as that in Example 9.

[0127] Comparative Example 1

[0128] The present application provides a kind of household routine disinfectant and preparation method thereof, the disinfectant includes the following weight fraction ratio of component:

[0129] The preparation method of the extract of forsythia is as follows: forsythia is decocted and extracted with 10 times water for 2 times, each time for 30 min; filter, combine filtrate, and concentrate to 1.25 g / ml, to obtain the extract of forsythia;

[0130] The preparation method of the extract of honeysuckle is the same as that in Example 1.

[0131] Comparative Example 2

[0132] The present application provides a kind of household routine disinfectant and preparation method thereof, the disinfectant includes the following weight fraction ratio of component:

[0133] The preparation method of the extract of forsythia is as follows: forsythia is heated and refluxed with 7 times 55% v / v ethanol aqueous solution for 2 times, each time for 30 min; filter, combine filtrate, and concentrate to 1.25 g / ml, to obtain the extract of forsythia;

[0134] The preparation method of the extract of honeysuckle is the same as that in Example 1.

[0135] Comparative Example 3

[0136] The present application provides a kind of household routine disinfectant and preparation method thereof, the disinfectant includes the following weight fraction ratio of component:

[0137] The preparation method of the extract of forsythia is as follows: forsythia is ultrasonically extracted with 7 times 20% v / v ethanol aqueous solution for 45 min, and the ultrasonic frequency is 45 Hz; filter, and concentrate the filtrate to 1.25 g / ml, to obtain the extract of forsythia;

[0138] The preparation method of the extract of honeysuckle is the same as that in Example 1.

[0139] Comparative Example 4

[0140] This comparative example provides a disinfectant composition having the same ingredients as Example 1.

[0141] wherein the preparation method of the Forsythia extract is the same as Example 1;

[0142] The preparation method of the honeysuckle extract is as follows: the honeysuckle is decocted and extracted with 10 times water for 2 times, each for 30 min; the filtrates are combined and concentrated to 1.25 g / ml, and then the honeysuckle extract is obtained.

[0143] Comparative Example 5

[0144] This comparative example provides a disinfectant composition having the same ingredients as Example 1.

[0145] wherein the preparation method of the Forsythia extract is the same as Example 1;

[0146] The preparation method of the honeysuckle extract is as follows: the honeysuckle is heated and refluxed with 7 times 57% v / v aqueous ethanol solution for 2 times, each for 30 min; the filtrates are combined and concentrated to 1.25 g / ml, and then the honeysuckle extract is obtained.

[0147] Comparative Example 6

[0148] This comparative example provides a disinfectant composition having the same ingredients as Example 1.

[0149] wherein the preparation method of the Forsythia extract is the same as Example 1;

[0150] The preparation method of the honeysuckle extract is as follows: the honeysuckle is ultrasonically extracted with 8 times 55% v / v aqueous ethanol solution for 45 min, and the ultrasonic frequency is 45 Hz; the filtrates are combined and concentrated to 1.25 g / ml, and then the honeysuckle extract is obtained.

[0151] Comparative Example 7

[0152] This comparative example provides a disinfectant composition having the following ingredients in parts by weight:

[0153] 0.1 part of the Sophora flavescens extract, 0.1 part of the honeysuckle extract, 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.

[0154] wherein the preparation method of the honeysuckle extract is the same as Example 1;

[0155] The preparation method of the sophora flavescens extract comprises the following steps: subjecting the sophora flavescens extract to heating reflux extraction using a 55% v / v ethanol aqueous solution whose pH is adjusted to 8.2 with sodium hydroxide, wherein the mass of the 55% v / v ethanol aqueous solution is 8 times the mass of the sophora flavescens; filtering after the extraction, concentrating the obtained filtrate, applying XDA-1 macroporous adsorption resin, eluting with a 90% v / v ethanol aqueous solution, and then eluting with water, collecting the water elution fraction, and concentrating it to 1.25 g / ml to obtain the sophora flavescens extract.

[0156] Comparative Example 8

[0157] This comparative example provides a disinfectant composition comprising the following components in parts by weight:

[0158] 0.1 part of Forsythia suspensa extract, 0.1 part of Sophora flavescens extract, 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.

[0159] The preparation method of the Forsythia suspensa extract is the same as that of Example 1, and the preparation method of the Sophora flavescens extract is the same as that of Comparative Example 7.

[0160] Comparative Example 9

[0161] This comparative example provides a disinfectant composition comprising the following components in parts by weight:

[0162] 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.

[0163] Comparative Example 10

[0164] This comparative example provides a disinfectant composition comprising the following components in parts by weight:

[0165] 0.45 parts of polyaminopropyl biguanide and 0.15 parts of benzalkonium chloride.

[0166] Comparative Example 11

[0167] This comparative example provides a disinfectant and a preparation method thereof. The composition ratio and preparation method of the disinfectant are the same as those in Example 9, wherein the preparation methods of the Forsythia suspensa extract and the honeysuckle flower extract are the same as those in Comparative Example 1.

[0168] Comparative Example 12

[0169] This comparative example provides a disinfectant and a preparation method thereof. The composition ratio and preparation method of the disinfectant are the same as those in Example 9, wherein the preparation methods of the Forsythia suspensa extract and the honeysuckle extract are the same as those in Comparative Example 2.

[0170] Comparative Example 13

[0171] The present comparative example provides a disinfectant solution and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspensa extract and the honeysuckle extract are the same as those of Comparative Example 3.

[0172] Comparative Example 14

[0173] The present comparative example provides a disinfectant solution and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspensa extract and the honeysuckle extract are the same as those of Comparative Example 4.

[0174] Comparative Example 15

[0175] The present comparative example provides a disinfectant solution and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspensa extract and the honeysuckle extract are the same as those of Comparative Example 5.

[0176] Comparative Example 16

[0177] The present comparative example provides a disinfectant solution and a preparation method thereof, the component ratio and the preparation method of which are the same as those of Example 9, wherein the preparation methods of the forsythia suspensa extract and the honeysuckle extract are the same as those of Comparative Example 6.

[0178] Comparative Example 17

[0179] The present comparative example provides a disinfectant solution and a preparation method thereof, the component ratio of which is based on that of Example 9, the forsythia suspensa extract is replaced by a sophora flavescens extract, and the preparation method is the same as that of Example 9. The preparation methods of the sophora flavescens extract and the honeysuckle extract are the same as those of Comparative Example 7.

[0180] Comparative Example 18

[0181] The present comparative example provides a disinfectant solution and a preparation method thereof, the component ratio of which is based on that of Example 9, the honeysuckle extract is replaced by a sophora flavescens extract, and the preparation method is the same as that of Example 9. The preparation methods of the sophora flavescens extract and the forsythia suspensa extract are the same as those of Comparative Example 8.

[0182] Comparative Example 19

[0183] The present comparative example provides a disinfectant solution and a preparation method thereof, the component ratio of which is based on that of Example 9, the forsythia suspensa extract and the honeysuckle extract are removed, and the preparation method is the same as that of Example 9.

[0184] Comparative Example 20

[0185] The comparative example 9 provides a disinfectant and a preparation method thereof. The disinfectant is prepared based on the composition ratio of the example 9, and the Forsythia extract, the honeysuckle extract, and the didecyldimethylammonium chloride are removed. The amount of the polyaminopropyl biguanide is changed to 0.45 parts, and the amount of the benzalkonium chloride is changed to 0.15 parts. The preparation method is the same as that of the example 9.

[0186] Test example 1

[0187] The disinfecting effects of the disinfecting compositions prepared in the examples 1 to 8 and the comparative examples 1 to 10 are investigated in this test example.

[0188] The composition ratio of the nutrient agar medium used in the following experiment is as follows: 10 g of proteose peptone, 3 g of beef extract, 5 g of sodium chloride, 17 g of agar, and 1000 mL of distilled water.

[0189] The composition of the Sabouraud medium is as follows: 10 g of proteose peptone, 20 g of agar, and 40 g of glucose, and 1 L of distilled water.

[0190] Test strains: Staphylococcus aureus (ATCC 6538), Escherichia coli (8099), and Candida albicans (ATCC 10231).

[0191] 1. Experimental method

[0192] The nutrient agar medium slant culture (24 h) of the test bacteria of the 5th generation is washed with 5 mL of 0.03 mol / L phosphate buffer (hereinafter referred to as PBS) to remove the bacterial mat, and is diluted with the PBS to prepare a bacterial suspension with a concentration of 5 x 10 4 cfu / mL.

[0193] The disinfecting composition samples prepared in the examples 1 to 8 and the comparative examples 1 to 10 are used as the test sample liquid. Four tubes of the test sample liquid (5 mL) and the control sample liquid (having the same composition as the test sample liquid, but not containing the Forsythia extract, the honeysuckle extract, the didecyldimethylammonium chloride, the polyaminopropyl biguanide, and the benzalkonium chloride, and being sterilized) are taken.

[0194] 100 μL of the above bacterial suspension is added dropwise into each of the test sample liquid and the control sample liquid, and is uniformly mixed. Timing is started, and the mixture is allowed to act for 2 min, 5 min, 10 min, 20 min, 1 h, and 2 h. The mixed sample (0.5 mL) is then taken out with sterile forceps into a test tube containing 5 mL of PBS, and is uniformly mixed. 0.5 mL of the mixture is taken out and is placed on two plates. Nutrient agar medium (for bacteria) or Sabouraud agar medium (for yeast) at a temperature of 40 to 50°C (15 mL) is poured into the plates, and the plates are rotated to uniformly spread the medium. After the agar is solidified, the plates are turned over, and are cultured at 35°C ± 2°C for 48 h (for bacteria) or 72 h (for yeast). The number of viable bacterial colonies is counted.

[0195] The test was repeated 3 times, and the bacteriostatic rate was calculated according to the following formula:

[0196] X = (A-B) / A x 100%;

[0197] In the formula, X is the bacteriostatic rate, A is the average number of bacteria of the control sample, and B is the average number of bacteria of the test sample.

[0198] 2. Experimental results.

[0199] The average bacteriostatic rate of each disinfecting composition at different action times was recorded, and the results are shown in Tables 1-3.

[0200] Table 1: Bacteriostatic data against Staphylococcus aureus

[0201]

[0202] Table 2: Bacteriostatic data against Escherichia coli

[0203]

[0204]

[0205] Table 3: Bacteriostatic data against Candida albicans

[0206]

[0207]

[0208] As can be seen from the test results in Tables 1-3, the bacteriostatic rates of the disinfecting compositions obtained in Examples 1-3 against Staphylococcus aureus, Escherichia coli, and Candida albicans are all >99.9% at 5 min-2 h, the disinfecting compositions obtained in Examples 4-8 also have good bacteriostatic effects against Staphylococcus aureus, Escherichia coli, and Candida albicans, and the bacteriostatic rates of the disinfecting compositions obtained in Examples 1-8 are all better than those of the disinfecting compositions obtained in the comparative examples.

[0209] Test Example 2

[0210] This test example investigates the irritability of the disinfecting solutions prepared in Examples 9-16 and Comparative Examples 11-20.

[0211] 1. Experimental method

[0212] Fifty-four New Zealand rabbits with perfect skin, female, weighing 2.0-2.5 kg, were fed with ordinary grade rabbit maintenance feed, at a temperature of 20-26°C and a relative humidity of 40%-70%.

[0213] According to the "Disinfection Technical Standard" (2002 edition) 2.3.3.3.1, the prepared disinfectant of Examples 9-16 and Comparative Examples 11-20 was respectively subjected to a complete skin irritation test (3 rabbits were used for each sample): about 24 hours before the experiment, the back of the rabbit was shaved on both sides of the spine, without damaging the skin, and the shaved area was about 3 cm x 3 cm on each side. 0.5 ml of the test solution was applied to the skin, then covered with two layers of gauze and one layer of glass paper, and fixed with adhesive tape. The other side of the skin was applied with 0.5 ml of distilled water as a blank control. The application time was 4 hours, and the test material was removed by washing with warm water for 1 min. The skin reaction was observed at 1 hour, 24 hours and 48 hours after the test material was removed, and the skin irritation reaction score was evaluated according to Table 4. The results are shown in Tables 5 and 6.

[0214] Table 4 Skin irritation reaction score criteria

[0215]

[0216]

[0217] Table 5 Test results of disinfectants obtained in Examples 9-16

[0218]

[0219]

[0220] Table 6 Test results of disinfectants obtained in Comparative Examples 11-20

[0221]

[0222]

[0223] From the results of Tables 5 and 6, it can be seen that the disinfectants obtained in Examples 9-16 had no skin irritation to white rabbits, and the disinfectants obtained in Comparative Examples 11-20 had skin irritation to white rabbits.

[0224] Test Example 3

[0225] This test example investigated the corrosion of the disinfectants obtained in Examples 9-16 on metals.

[0226] 1. Preparation of metal pieces: 80 pieces of stainless steel pieces (304), aluminum pieces and copper pieces were prepared, each with a surface area of 10 ± 0.2 cm 2 . The surface oxide layer was removed by sanding with a 120-grit sandpaper, then soaked in soap water for 10 min, rinsed with tap water and then distilled water, and dried.

[0227] 2. The above metal sheets were randomly divided into 8 groups, 10 pieces in each group, and were immersed in the disinfectant solutions obtained in Examples 9-16, and R value determination was carried out according to GB10124-88 "Metal Material Laboratory Uniform Corrosion Full Immersion Test Method" at 20-25°C. The results are shown in Table 7.

[0228] Table 7 Metal corrosion test results of the disinfectant solutions obtained in Examples 9-16

[0229]

[0230] As can be seen from the results in Table 7, the disinfectant solutions obtained in Examples 9-16 have no corrosion on the above metal sheets.

[0231] As can be seen from the results in Test Examples 1, 2 and 3, the disinfectant composition provided in the examples of the present application has excellent disinfecting effect and has no corrosion on metal, and is mild and non-irritating to the skin compared with the comparative examples.

[0232] The above description is merely preferred embodiments of the present application, and is not intended to limit the present application. Any modification, equivalent replacement or improvement made within the spirit and principle of the present application shall fall within the scope of protection of the present application.

Claims

1. A disinfectant composition, characterized in that The invention is composed of the following ingredients in parts by weight: 0.1-1 part of forsythia extract, 0.1-1 part of honeysuckle extract, 0.05-0.15 part of didecyldimethylammonium chloride, 0.1-0.5 part of polyaminopropyl biguanide and 0.05-0.5 part of benzalkonium chloride; The preparation method of the Forsythia suspensa extract is as follows: The forsythia suspensa is subjected to heating reflux extraction with a 45% to 65% v / v ethanol aqueous solution adjusted to a pH of 8 to 8.5 with sodium hydroxide; after the extraction is completed, the filtrate is filtered and concentrated, and the filtrate is applied to a polar macroporous adsorption resin, eluted with an 85% to 95% v / v ethanol aqueous solution, and then eluted with water, the water elution fraction is collected and concentrated to 1.2 to 1.3 g / ml to obtain the forsythia suspensa extract; wherein the mass of the 45% to 65% v / v ethanol aqueous solution is 5 to 8 times the mass of the forsythia suspensa; The preparation method of the honeysuckle extract is: The honeysuckle is subjected to heating reflux extraction with a 55-60% v / v ethanol aqueous solution adjusted to pH 8-8.5 with sodium hydroxide, filtered, and the resulting filter residue is extracted with a 25-35% v / v ethanol aqueous solution under heating reflux, filtered, and the filtrate is combined and concentrated to 1.2-1.3 g / ml to obtain the honeysuckle extract.

2. The disinfectant composition according to claim 1, characterized in that The invention is composed of the following ingredients in parts by weight: 0.1-0.3 parts of forsythia extract, 0.1-0.3 parts of honeysuckle extract, 0.05-0.1 parts of didecyldimethylammonium chloride, 0.3-0.5 parts of polyaminopropyl biguanide and 0.05-0.15 parts of benzalkonium chloride.

3. The disinfectant composition according to claim 2, characterized in that The invention is composed of the following ingredients in parts by weight: 0.1 part of forsythia extract, 0.1 part of honeysuckle extract, 0.1 part of didecyldimethylammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.

4. Use of the disinfectant composition according to any one of claims 1 to 3 in the preparation of daily household disinfection products.

5. A disinfectant, characterized in that: The disinfectant composition comprises the disinfectant composition according to any one of claims 1 to 3.

6. The disinfectant according to claim 5, characterized in that The composition comprises the following components in parts by weight: 80-95 parts water; 3~6 parts of moisturizer; 0.05~0.2 parts of antioxidant; 0.5~1 part of preservative; 0.05~0.3 parts of flavor; 0.05~0.3 parts of citric acid; Solubilizer 0.02~0.3 parts; 0.1~1 part of Forsythia suspensa extract; 0.1~1 part of honeysuckle extract; 0.05-0.15 parts of didecyldimethylammonium chloride; Polyaminopropyl biguanide 0.1-0.5 parts; 0.05~0.5 parts of benzalkonium chloride.

7. The disinfectant according to claim 6, characterized in that The moisturizing agent is selected from at least one of propylene glycol, glycerin and butylene glycol; and / or The preservative is phenoxyethanol; and / or The solubilizing agent is PEG-40 hydrogenated castor oil; and / or The antioxidant is disodium ethylenediaminetetraacetic acid.

8. The method for preparing the disinfectant according to claim 6 or 7, characterized in that: The following steps are involved: adding the moisturizing agent to the water and stirring until dissolved; Add the didecyldimethylammonium chloride, polyaminopropyl biguanide and benzalkonium chloride, and stir to disperse evenly; Add the forsythia extract, honeysuckle extract, citric acid, essence, solubilizer, antioxidant and preservative, mix evenly, and obtain the disinfectant.

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