A probiotics culture device

Abstract

The invention belongs to the field of probiotic culture equipment, and is a probiotic culture equipment, a probiotic culture equipment, including a shell, characterized in that a sealing plate is provided on the shell, a sealing device is provided on the sealing plate, the sealing device is used to isolate the inside of the cavity from the outside to ensure the airtightness of the inside of the cavity, a collection cavity is fixedly provided on the lower side wall of the shell, and a tilting device is provided on the collection cavity, and the tilting device is used to shake the cavity inside the cavity. This device is designed to be a probiotic culture device that can autonomously control the sealing conditions of the cavity, can monitor the inside of the cavity while sealing the inside of the cavity, and dynamically adjust the balance according to the changes in the cavity to ensure that a good breeding environment is provided for the probiotics. A timed tilting device can be designed inside the culture medium cavity, and the inside of the culture medium can be stirred, and the equipment for detecting colonies is provided with a switch to prevent excessive oxygen from entering the cavity.

Description

Technical Field

[0001] The invention belongs to the field of probiotics culture equipment, and in particular relates to a probiotics culture equipment. Background Art

[0002] As we all know, probiotics can synthesize digestive enzymes. Together with the digestive enzymes synthesized by animals, they participate in the digestion of nutrients in the intestines, stimulate the animal body to secrete digestive enzymes, reduce the depth of small intestinal crypts, increase the height of villi, increase the surface area of ​​the small intestine, and promote the absorption of intestinal nutrients. The self-structure of probiotics, such as peptidoglycan, lipoteichoic acid and other components, can directly play an immune activation role as antigens, or stimulate the host immune system through autocrine immune activators, thereby improving the immunity of animals, enhancing the activity of the body's inherent immune cells and natural killer cells, activating dendritic cells, stimulating the body to produce cytokines, and stimulating B cells to secrete antibodies. Probiotics can stimulate the intestine to produce secretory globulin A to protect the body's health.

[0003] Probiotics are beneficial to health and have good development prospects in the future.

[0004] This equipment is designed to be a probiotic incubator that can autonomously control the sealing conditions of the cavity. It can monitor the interior of the cavity while sealing it, and make dynamic balance adjustments based on changes in the cavity to ensure that a good breeding environment is provided for probiotics.

[0005] A timed tilting device can be designed inside the culture medium cavity to stir the inside of the culture medium, which can make it easier for probiotics to grow.

[0006] In addition, a device for detecting colonies at any time is provided on the side of the equipment, and a switch for sealing the cavity is provided on it to prevent excessive oxygen from entering the cavity when sampling, thereby blocking oxygen for anaerobic probiotics. Summary of the invention

[0007] The purpose of the present invention is to address the above-mentioned problems in the existing technology and to propose a probiotic culture device. The probiotic culture device can dynamically balance the internal parameters when the cavity is sealed, thereby providing a good environment for the reproduction of probiotics.

[0008] The objective of the present invention can be achieved through the following technical scheme: a probiotic culture device, comprising a shell, characterized in that a sealing plate is provided on the shell, a sealing device is provided on the sealing plate, the sealing device is used to isolate the inside of the cavity from the outside to ensure the airtightness of the inside of the cavity, a collecting chamber is fixedly provided on the lower side wall of the shell, a tilting device is provided on the collecting chamber, the tilting device is used to shake the inside of the cavity, the tilting device comprises a motor frame rotating on the collecting chamber, a motor is fixedly provided in the motor frame, an output shaft is provided at the output end of the motor, a stirring device is provided on the output shaft, the stirring device is used to stir the nutrient medium for bacterial culture, and a detection device is provided on the left and right side walls of the shell, the detection device is used to detect the colony inside the cavity.

[0009] Furthermore, the sealing device includes a thick sealing gasket fixed on the lower side wall of the sealing plate, and a balancing device is provided on the thick sealing gasket, which is used to adjust the dynamic balance of various parameters inside the cavity. A locking pin is provided on the sealing plate, and a clamping device is provided on the outside of the locking pin, which is used to ensure the stability of the sealing device and clamp the stabilizing device.

[0010] Furthermore, the balancing device includes a gas controller fixed on the sealing plate, a humidifier and cooler is fixed on the sealing plate, pipes are provided at the lower ends of the gas controller and the humidifier and cooler, the pipes extend downward and extend into the interior of the thick sealing gasket, a lead screw is fixed inside the thick sealing gasket, an electric slider is slidably provided on the lead screw, a detector is fixed inside the lower side wall of the thick sealing gasket, the detector is used to detect the air pressure, temperature and humidity inside the cavity and transmit electrical signals to the electric slider, the electric slider is operated to slide on the lead screw, an opening is provided on the electric slider, when the opening coincides with the pipe, the cavity is connected with the gas controller and the humidifier and cooler.

[0011] Furthermore, the clamping device includes a clamping slider sliding on the lower end of the sealing plate, the clamping slider can slide into the side wall of the shell, a rotating push rod is rotatably provided in the clamping slider, a bevel gear is provided at the proximal axial end of the rotating push rod, a changing cavity is provided at the proximal axial end of the clamping slider, a bevel gear is rotatably provided in the changing cavity, a clamping block is rotatably provided on the bevel gear, the bevel gear can mesh with the proximal axial end of the rotating push rod for transmission, and when the bevel gear rotates, it will drive the clamping block to rotate, and the clamping block will be ejected outward or retracted.

[0012] Furthermore, the tilting device includes a tilting frame fixedly provided on the collection chamber, a slide groove provided on the tilting frame, a culture vessel slidably provided on the tilting frame, the culture vessel can swing back and forth on the tilting frame, and a recovery device is provided on the collection chamber, and the recovery device is used to recover excess water generated during the bacterial culture process.

[0013] Furthermore, the recovery device includes a water collector fixed on the collection chamber, and a water-permeable membrane is fixed on the lower side wall of the culture vessel. Excess water can pass through the water-permeable membrane and flow into the collection chamber through the water collector.

[0014] Furthermore, the stirring device includes a differential fixed on the output shaft, a shaft sleeve is fixedly provided on the outside of the differential, and the differential can achieve different rotation frequencies of the shaft sleeve and the output shaft. A gear transmission box is fixed on the shaft sleeve, and stirring blades are rotatably provided on the outside of the gear transmission box. The rotational force on the output shaft is transmitted to the stirring blades through the gear transmission box.

[0015] Furthermore, the detection device includes an outer detection slide bar sliding on the left and right side walls of the shell, a detection push rod is slidably provided inside the outer detection slide bar, a connecting plate is fixedly provided on the left and right inner side walls of the shell, a transmission cavity block is provided in the connecting plate, a follower wheel is rotatably provided in the transmission cavity block, a winding wheel is rotatably provided in the incubator, an open slide bar is slidably provided in the incubator, a bearing is fixedly provided in the incubator, a changing transmission rod is rotatably provided on the bearing, a rope is connected between the opening slide bar, the winding wheel and the changing transmission rod, and the outer detection slide bar can drive the following wheel to rotate.

[0016] When using this equipment to cultivate probiotics, the culture medium is placed inside the incubator, the inside of the shell is sealed by a sealing plate, the internal air is adjusted by a humidifier and cooler, oxygen is extracted and nitrogen is filled in, and the internal air pressure, temperature, humidity and other parameters are constantly detected, and the parameters inside the shell that are useful for cultivating probiotics are automatically adjusted. The probiotics inside the incubator can be detected by sliding the outer slide rod inward and extending the detection push rod into the incubator to ensure that the cultured probiotics meet the standards. The incubator is tilted and stirred during the probiotic cultivation process to prevent problems such as excessive temperature at the center of the internal culture medium. Excess moisture is filtered out during the stirring process to prevent excessive moisture from affecting the reproduction of probiotics.

[0017] When the sealing device is used, the rotating push rod is pushed to push the clamping slider as a whole into the side wall of the shell, and the rotating push rod is rotated. When the rotating push rod rotates, it will drive the bevel gear to rotate. The rotation of the bevel gear will pop out the card block, and the card block will clamp the clamping slider in the shell. The clamping slider will also clamp the locking pin to prevent the locking pin from sliding up and down in the vertical direction, so that the sealing plate covers and seals the shell. After sealing, the detector is started to dynamically monitor the inside of the cavity. When the temperature inside the shell is too high, the electric slider slides to the right, and the opening on the electric slider is connected to the pipeline under the humidifier and cooler, and atomized water vapor is sprayed inside the cavity to cool down. When the air pressure inside the shell is unstable, the electric slider slides to the left, and the opening of the electric slider is connected to the pipeline under the gas controller. The dynamic balance of the air pressure inside the shell is controlled by the gas controller to prevent nitrogen from leaking out during detection, resulting in external oxygen entering, which can ensure the sealing effect inside the device and maintain the dynamic balance effect inside the device, and can provide a better survival and reproduction environment for probiotics.

[0018] Set a fixed time interval to tilt the incubator. The incubator rocking back and forth on the tilting frame is conducive to the excess water flowing downward, and flowing into the collection chamber through the water collector. Anaerobic respiration will occur and water will be produced during the reproduction of probiotics, which needs to be collected and the motor is started. The open skateboard motor drives the output shaft to rotate, and the differential effect of the differential is used to control the speed of the sleeve, so that the rotation speed difference between the sleeve and the output shaft is achieved, and the output shaft rotation efficiency is faster than that of the sleeve. When the output shaft rotates, it will be transmitted through the gear transmission box, which will drive the stirring blades to rotate. The rotation of the stirring blades will stir the culture medium inside the incubator to avoid excessively high center temperature of the culture medium inside the incubator, which will lead to poor reproduction of probiotics.

[0019] During the reproduction of probiotics, the colony is detected, and the outer sliding rod of the detection slides as a whole toward the inside of the shell. During the sliding process of the outer sliding rod of the detection, the follower wheel will be driven to rotate, and the rotation of the follower wheel will drive the change-direction transmission rod to rotate. The change-direction transmission rod will tighten the rope. After the rope is tightened, the open slide plate will slide away from the axis end, and the outer sliding rod of the detection can enter the interior of the incubator. Then the detection push rod is extended into the interior of the incubator to sample the bacterial colonies in the incubator. This can ensure that during the process of culturing probiotics, sampling and detection are carried out at regular intervals and at fixed points to ensure the quality and quantity of probiotic culture and avoid culturing too much or too little probiotics. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the appearance structure of the probiotics cultivation equipment.

[0021] Figure 2 It is an internal cross-sectional view of a probiotic culture device.

[0022] Figure 3 yes Figure 2 Section view along AA direction.

[0023] Figure 4 yes Figure 2 Enlarged view of the structure at point B in the middle.

[0024] Figure 5 yes Figure 3 Enlarged view of the structure at point C in the middle.

[0025] In the figure, 10, shell; 11, incubator; 12, gas controller; 13, humidifier and cooler; 14, pipeline; 15, lead screw; 16, electric slider; 17, detector; 18, sealing plate; 19, locking pin; 20, thick sealing gasket; 21, clamping slider; 22, rotating push rod; 23, changing cavity; 24, bevel gear; 25, block; 26, detection outer slide rod; 27, detection push rod; 28, transmission cavity block; 29, follower wheel; 30, changing transmission rod; 31, rope; 32, winding wheel; 33, open slide plate; 34, bearing; 35, connecting plate; 36, collecting cavity; 37, motor frame; 38, motor; 39, differential; 40, bushing; 41, output shaft; 42, water permeable membrane; 43, tilting frame; 44, water collector; 45, stirring blade; 46, gear transmission box. DETAILED DESCRIPTION

[0026] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

[0027] like Figure 1 As shown, a probiotic culture device includes a shell 10, characterized in that a sealing plate 18 is provided on the shell 10, and a sealing device is provided on the sealing plate 18, the sealing device is used to isolate the inside of the cavity from the outside to ensure the airtightness of the inside of the cavity, a collecting chamber 36 is fixedly provided on the lower side wall of the shell 10, and a tilting device is provided on the collecting chamber 36, the tilting device is used to shake the inside of the cavity, the tilting device includes a motor frame 37 rotating on the collecting chamber 36, a motor 38 is fixedly provided in the motor frame 37, an output shaft 41 is provided at the output end of the motor 38, and a stirring device is provided on the output shaft 41, the stirring device is used to stir the nutrient medium for bacterial culture, and a detection device is provided on the left and right side walls of the shell 10, and the detection device is used to detect the colony inside the cavity.

[0028] like Figure 2 and Figure 3As shown, the sealing device includes a thick sealing gasket 20 fixed on the lower side wall of the sealing plate 18, and a balancing device is provided on the thick sealing gasket 20, and the balancing device is used to adjust the dynamic balance of various parameters inside the cavity. A locking pin 19 is provided on the sealing plate 18, and a clamping device is provided on the outer side of the locking pin 19, and the clamping device is used to ensure the stability of the sealing device and clamp the stabilizing device.

[0029] like Figure 2 , Figure 3 and Figure 5 As shown, the balancing device includes a gas controller 12 fixed on a sealing plate 18, a humidifier and cooler 13 is fixed on the sealing plate 18, a pipe 14 is provided at the lower ends of the gas controller 12 and the humidifier and cooler 13, the pipe 14 extends downward and extends into the inside of a thick sealing gasket 20, a lead screw 15 is fixed inside the thick sealing gasket 20, an electric slider 16 is slidably provided on the lead screw 15, a detector 17 is fixed inside the lower side wall of the thick sealing gasket 20, the detector 17 is used to detect the air pressure, temperature and humidity inside the cavity and transmit electrical signals to the electric slider 16, the electric slider 16 is operated to slide on the lead screw 15, an opening is provided on the electric slider 16, when the opening coincides with the pipe 14, the cavity is connected with the gas controller 12 and the humidifier and cooler 13.

[0030] like Figure 2 , Figure 3 and Figure 5 As shown, the clamping device includes a clamping slider 21 sliding on the lower end of the sealing plate 18, and the clamping slider 21 can slide into the side wall of the shell 10. A rotating push rod 22 is rotatably provided in the clamping slider 21, and a bevel tooth is provided at the proximal axial end of the rotating push rod 22. A changing cavity 23 is provided at the proximal axial end of the clamping slider 21, and a bevel gear 24 is rotatably provided in the changing cavity 23. A clamping block 25 is rotatably provided on the bevel gear 24, and the bevel gear 24 can mesh with the proximal axial end of the rotating push rod 22 for transmission. When the bevel gear 24 rotates, it will drive the clamping block 25 to rotate, and the clamping block 25 will be ejected outward or retracted.

[0031] like Figure 2 and Figure 3 As shown, the tilting device includes a tilting frame 43 fixed on the collecting chamber 36, a slide groove is provided on the tilting frame 43, a culture vessel 11 is slidably provided on the tilting frame 43, and the culture vessel 11 can swing back and forth on the tilting frame 43. A recovery device is provided on the collecting chamber 36, and the recovery device is used for excess water generated during the bacterial culture process.

[0032] like Figure 3 As shown, the recovery device includes a water collector 44 fixed on the collection chamber 36, and a water-permeable membrane 42 is fixed on the lower side wall of the culture vessel 11. Excess water can pass through the water-permeable membrane 42 and flow into the collection chamber 36 through the water collector 44.

[0033] like Figure 3As shown, the stirring device includes a differential 39 fixed on the output shaft 41, a shaft sleeve 40 is fixedly provided on the outer side of the differential 39, and the shaft sleeve 40 and the output shaft 41 can have different rotation frequencies through the differential 39. A gear transmission box 46 is fixed on the shaft sleeve 40, and stirring blades 45 are rotatably provided on the outer side of the gear transmission box 46. The rotational force on the output shaft 41 is transmitted to the stirring blades 45 through the gear transmission box 46.

[0034] like Figure 2 , Figure 3 and Figure 4 As shown, the detection device includes a detection outer slide bar 26 that slides on the left and right side walls of the shell 10, a detection push rod 27 is slidably provided inside the detection outer slide bar 26, a connecting plate 35 is fixedly provided on the left and right inner walls of the shell 10, a transmission cavity block 28 is provided in the connecting plate 35, a follower wheel 29 is rotatably provided in the transmission cavity block 28, a winding wheel 32 is rotatably provided in the incubator 11, an open slide plate 33 is slidably provided in the incubator 11, a bearing 34 is fixedly provided in the incubator 11, a change-of-direction transmission rod 30 is rotatably provided on the bearing 34, a rope 31 is connected between the open slide plate 33, the winding wheel 32 and the change-of-direction transmission rod 30, and the detection outer slide bar 26 can drive the follower wheel 29 to rotate.

[0035] When using this device to cultivate probiotics, the culture medium is placed inside the culture vessel 11, the inside of the shell 10 is sealed by the sealing plate 18, and the internal air is adjusted by the humidifier and cooler 13, oxygen is extracted and nitrogen is filled in, and the internal air pressure, temperature, humidity and other parameters are detected at all times, and the parameters inside the shell 10 that are useful for cultivating probiotics are automatically adjusted. The outer sliding rod 26 can be slid inward to extend the detection push rod 27 into the culture vessel 11 to detect the probiotics inside the culture vessel 11 to ensure that the cultured probiotics meet the standards. During the probiotic cultivation process, the culture vessel 11 is tilted and stirred to prevent problems such as excessive temperature at the center of the internal culture medium. At the same time, excess moisture is filtered out during the stirring process to prevent excessive moisture from affecting the reproduction of probiotics.

[0036] When the sealing device is used, the rotating push rod 22 is pushed to push the clamping slider 21 as a whole into the side wall of the shell 10, and the rotating push rod 22 is rotated. When the rotating push rod 22 rotates, it will drive the bevel gear 24 to rotate. The rotation of the bevel gear 24 will pop out the block 25, and the block 25 will clamp the clamping slider 21 in the shell 10. The clamping slider 21 will also clamp the locking pin 19 to prevent the locking pin 19 from sliding up and down in the vertical direction, so that the sealing plate 18 covers and seals the shell 10. After sealing, the detector 17 is started to dynamically monitor the inside of the cavity. When the internal temperature of the shell 10 is too high, , the electric slider 16 slides to the right, and the opening on the electric slider 16 is connected to the pipe 14 under the humidifier and cooler 13, so as to spray atomized water vapor inside the cavity to cool it down. When the air pressure inside the shell 10 is unstable, the electric slider 16 slides to the left, and the opening of the electric slider 16 is connected to the pipe 14 under the gas controller 12. The gas controller 12 controls the dynamic balance of the air pressure inside the shell 10 to prevent nitrogen from leaking out during detection, causing external oxygen to enter, thereby ensuring the sealing effect inside the device and maintaining the dynamic balance effect inside the device, and providing a better living and breeding environment for probiotics.

[0037] The incubator 11 is tilted at a fixed time interval. The incubator 11 is rocked back and forth on the tilting frame 43, which is conducive to the excess water flowing downward and flowing into the collection chamber 36 through the water collector 44. During the reproduction of probiotics, anaerobic respiration will occur and water will be produced, which needs to be collected, and the motor 38 is started. The motor 38 drives the output shaft 41 to rotate, and the differential effect of the differential 39 is used to control the rotation speed of the sleeve 40, so that there is a speed difference between the sleeve 40 and the output shaft 41, and the output shaft 41 rotates faster than the sleeve 40. When the output shaft 41 rotates, it will be transmitted through the gear transmission box 46, which will drive the stirring blades 45 to rotate. The rotation of the stirring blades 45 will stir the culture medium inside the incubator 11 to avoid the central temperature of the culture medium inside the incubator 11 being too high, resulting in poor reproduction of probiotics.

[0038] During the reproduction of probiotics, the colony is detected, and the outer sliding rod 26 slides as a whole into the shell 10. During the sliding process of the outer sliding rod 26, the follower wheel 29 will be driven to rotate, and the rotation of the follower wheel 29 will drive the change-direction transmission rod 30 to rotate. The change-direction transmission rod 30 will tighten the rope 31. After the rope 31 is tightened, the open slide plate 33 will slide away from the axial end, and the outer sliding rod 26 can enter the interior of the incubator 11. Then the detection push rod 27 is extended into the interior of the incubator 11 to sample the bacterial colonies in the incubator 11. This can ensure that during the process of culturing probiotics, sampling and detection are carried out at regular intervals and at fixed points to ensure the quality and quantity of probiotic culture and avoid culturing too many or too few probiotics.

[0039] Compared with the existing technology, this probiotics culture equipment has the following advantages:

[0040] 1. Prevent nitrogen from leaking out during testing, causing external oxygen to enter, which can ensure the sealing effect inside the device and maintain the dynamic balance inside the device, providing a better living and breeding environment for probiotics.

[0041] 2. The rotation of the stirring blades will stir the culture medium inside the incubator to prevent the center temperature of the culture medium inside the incubator from being too high, which will lead to poor reproduction of probiotics.

[0042] 3. Sampling the bacterial colonies inside the culture vessel can ensure that sampling and testing are carried out at regular intervals and at fixed points during the cultivation of probiotics, thereby ensuring the quality and quantity of probiotic cultivation and avoiding the situation of culturing too much or too little probiotics.

[0043] The above descriptions are merely embodiments of the present invention, and are not intended to limit the patent protection scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention's specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A probiotics culture device, comprising a housing (10), characterized in that: The shell (10) is provided with a sealing plate (18), and the sealing plate (18) is provided with a sealing device, the sealing device is used to isolate the inside of the cavity from the outside to ensure the airtightness of the inside of the cavity; a collecting cavity (36) is fixedly provided on the lower side wall of the shell (10), and the collecting cavity (36) is provided with a tilting device, the tilting device is used to shake the inside of the cavity, the tilting device includes a motor frame (37) rotating on the collecting cavity (36), a motor (38) is fixedly provided in the motor frame (37), an output shaft (41) is provided at the output end of the motor (38), and a stirring device is provided on the output shaft (41), the stirring device is used to stir the nutrient medium for bacterial culture; the left and right side walls of the shell (10) are provided with detection devices, and the detection device is used to detect the bacterial colony inside the cavity; The tilting device comprises a tilting frame (43) fixedly provided on the collection chamber (36), the tilting frame (43) being provided with a slide groove, a culture vessel (11) being slidably provided on the tilting frame (43), the culture vessel (11) being able to swing back and forth on the tilting frame (43), and a recovery device being provided on the collection chamber (36); The recovery device comprises a water collector (44) fixed on the collection chamber (36), and a water-permeable membrane (42) is fixed on the lower side wall of the culture vessel (11), and excess water can pass through the water-permeable membrane (42) and flow into the interior of the collection chamber (36) through the water collector (44); The detection device comprises an outer detection slide bar (26) sliding on the left and right side walls of the shell (10), a detection push rod (27) slidingly arranged inside the outer detection slide bar (26), a connecting plate (35) fixedly arranged on the left and right inner side walls of the shell (10), a transmission cavity block (28) arranged inside the connecting plate (35), a follower wheel (29) rotatably arranged inside the transmission cavity block (28), a winding wheel (32) rotatably arranged inside the incubator (11), an open slide bar (33) slidingly arranged inside the incubator (11), a bearing (34) fixedly arranged inside the incubator (11), a direction-changing transmission rod (30) rotatably arranged on the bearing (34), a rope (31) connecting the open slide bar (33), the winding wheel (32) and the direction-changing transmission rod (30), and the outer detection slide bar (26) can drive the follower wheel (29) to rotate.

2. A probiotics culture device according to claim 1, characterized in that: The sealing device comprises a thick sealing gasket (20) fixed on the lower side wall of the sealing plate (18), a balancing device is provided on the thick sealing gasket (20), and the balancing device is used to adjust the dynamic balance of parameters inside the cavity, a locking pin (19) is provided on the sealing plate (18), and a clamping device is provided on the outer side of the locking pin (19), and the clamping device is used to ensure the stability of the sealing device and clamp the sealing device.

3. A probiotics culture device according to claim 2, characterized in that: The balancing device comprises a gas controller (12) fixed on the sealing plate (18), a humidifier and cooler (13) fixed on the sealing plate (18), a pipe (14) being provided at the lower ends of the gas controller (12) and the humidifier and cooler (13), the pipe (14) extending downward and extending into the interior of the thick sealing pad (20), a lead screw (15) being fixed inside the thick sealing pad (20), an electric slider (16) being slidably provided on the lead screw (15), a detector (17) being fixed inside the lower side wall of the thick sealing pad (20), the detector (17) being used to detect the air pressure, temperature and humidity inside the cavity and transmit an electric signal to the electric slider (16), so as to operate the electric slider (16) to slide on the lead screw (15), an opening being provided on the electric slider (16), and when the opening coincides with the pipe (14), the cavity is connected with the gas controller (12) and the humidifier and cooler (13).

4. A probiotics culture device according to claim 3, characterized in that: The clamping device comprises a clamping slider (21) sliding on the lower end of the sealing plate (18), the clamping slider (21) can slide into the side wall of the shell (10), a rotating push rod (22) is rotatably provided in the clamping slider (21), the near-axial end of the rotating push rod (22) is provided with bevel teeth, the near-axial end of the clamping slider (21) is provided with a changing cavity (23), a bevel gear (24) is rotatably provided in the changing cavity (23), a clamping block (25) is rotatably provided on the bevel gear (24), the bevel gear (24) can mesh with the near-axial end of the rotating push rod (22) for transmission, and when the bevel gear (24) rotates, it drives the clamping block (25) to rotate, and the clamping block (25) is ejected outward or retracted.

5. A probiotics culture device according to claim 4, characterized in that: The stirring device comprises a differential (39) fixed on the output shaft (41), a shaft sleeve (40) being fixedly arranged outside the differential (39), and the shaft sleeve (40) and the output shaft (41) can have different rotation frequencies through the differential (39), a gear transmission box (46) being fixedly arranged on the shaft sleeve (40), and stirring blades (45) being rotatably arranged outside the gear transmission box (46), and the rotational force on the output shaft (41) is transmitted to the stirring blades (45) through the gear transmission box (46).

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