Animal blood cell analysis method, analyzer, and storage medium

Abstract

An animal blood cell analysis method is applied to an animal blood cell analyzer (100). The method includes: selecting a target measurement mode from multiple animal measurement modes, and determining a target dose of diluent based on the target measurement mode (401); mixing the target dose of diluent with the blood of the animal to be tested and performing hemolysis to obtain a test sample (402); and testing the test sample to obtain white blood cell count and classification results (403). An animal blood cell analyzer (100) and a storage medium are also disclosed.

Description

Technical Field

[0001] The embodiments of the present invention relate to animal blood cell analysis technology, including but not limited to an animal blood cell analysis method, analyzer, and storage medium. Background Technology

[0002] The main principle of animal blood cell analysis is to test parameters such as white blood cells, red blood cells, and platelets through microwells on diluted animal blood samples.

[0003] An animal blood cell analyzer needs to test blood samples from various animals, including dogs, cats, rats, mice, and rabbits. However, the hemolysis process of leukocyte channels differs among these animals. Therefore, in related technologies, the accuracy of leukocyte count and classification results cannot be guaranteed using a single animal blood cell analyzer. Summary of the Invention

[0004] This invention provides a method, analyzer, and storage medium for analyzing animal blood cells, thereby improving the accuracy of white blood cell counts and classification results for different types of animals.

[0005] This invention provides a method for analyzing animal blood cells, applied to an animal blood cell analyzer, the method comprising:

[0006] Select the target measurement mode from multiple animal measurement modes, and determine the target dose of the diluent based on the target measurement mode;

[0007] The target dose of diluent is mixed with the blood of the animal to be tested and hemolyzed to obtain the test sample;

[0008] The test sample was tested to obtain white blood cell count and classification results.

[0009] This invention provides an animal blood cell analyzer, the animal blood cell analyzer comprising:

[0010] A sampling needle assembly is used to draw blood from the animal to be tested and deliver it to the first counting chamber assembly;

[0011] The mode selection module is used to select a target measurement mode from a variety of animal measurement modes and determine the target dose of the diluent based on the target measurement mode.

[0012] A reagent delivery assembly is used to mix the target dose of diluent with the blood of the animal to be tested and to perform hemolysis to obtain a test sample;

[0013] The first counting pool component is used to test the test sample to obtain white blood cell count and classification results.

[0014] This invention provides a storage medium storing an executable program. When the executable program is executed by a processor, it implements the steps of the animal blood cell analysis method performed by the aforementioned animal blood cell analyzer.

[0015] In this embodiment of the invention, the target dose of the diluent used to dilute the animal blood is determined based on the animal measurement mode corresponding to the animal species. The target dose of the diluent is used to dilute the blood of the animal to be tested. Thus, when testing the blood of the animal to be tested, the corresponding dose of the diluent is used to dilute the blood of the animal to be tested for different species. By controlling the dose of the diluent, the accuracy of the test results for white blood cells such as lymphocytes is improved. Attached Figure Description

[0016] Figure 1 A schematic diagram of an optional animal blood cell analyzer architecture provided for an embodiment of the present invention;

[0017] Figure 2 This is a schematic diagram illustrating the principle of impedance counting test provided in an embodiment of the present invention;

[0018] Figure 3 This is a schematic diagram of the white blood cell curve distribution provided in an embodiment of the present invention;

[0019] Figure 4 A schematic flowchart of an optional animal blood cell analysis method provided for an embodiment of the present invention;

[0020] Figure 5 A schematic diagram of an optional animal blood cell analyzer architecture provided for an embodiment of the present invention;

[0021] Figure 6 A schematic flowchart of an optional animal blood cell analysis method provided in an embodiment of the present invention. Detailed Implementation

[0022] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the embodiments provided herein are merely illustrative and not intended to limit the invention. Furthermore, the embodiments provided below are partial embodiments for implementing the present invention, not all embodiments for implementing the present invention. Unless otherwise specified, the technical solutions described in the embodiments of the present invention can be implemented in any combination.

[0023] In various embodiments of the present invention: the animal blood cell analyzer selects a target measurement mode from multiple animal measurement modes and determines the target dose of the diluent according to the target measurement mode; the target dose of the diluent is mixed with the blood of the animal to be tested and hemolyzed to obtain a test sample; and the test sample is tested to obtain white blood cell count and classification results.

[0024] This invention provides a method for analyzing animal blood cells, which is applied to an animal blood cell analyzer, such as... Figure 1 As shown, the animal blood cell analyzer 100 includes: a sampling needle assembly 101, a mode selection module 102, a reagent delivery assembly 103, and a first counting cell assembly 104. The sampling needle assembly 101 includes a sampling needle and a syringe for drawing blood from the animal to be tested and injecting the drawn blood into the first counting cell assembly. The mode selection module 102, implemented by the processor in the animal blood cell analyzer, is used to determine the current animal measurement mode. The reagent delivery assembly 103 controls the amount and timing of reagents such as diluents and lysing agents entering the first counting cell assembly of the animal blood cell analyzer. It includes a syringe and a liquid path, wherein the liquid path is located between the storage location for diluents and lysing agents and the first counting cell assembly, for transferring the diluents and lysing agents. The first counting cell assembly 104 is capable of performing white blood cell classification and counting tests on blood samples from different species of animals in the first counting cell assembly.

[0025] Blood samples, including white blood cells, red blood cells, and platelets, are obtained by processing whole blood drawn from an animal with a diluent and a hemolytic agent. The diluent is an isotonic solution with appropriate ionic strength and conductivity, possessing acid-base buffering properties. For example, the diluent may be primarily composed of hypoxanthine or xanthine compounds or their salts, or other diluents capable of performing the aforementioned functions. The hemolytic agent is used to dissolve red blood cells for white blood cell classification and counting. Hemolytic agents include surfactants, specifically cationic and nonionic surfactants. The amount and concentration of the hemolytic agent used can be rationally selected based on actual sample preparation requirements and are not specifically limited here. The hemolytic agent may be primarily composed of quaternary ammonium salt ionic surfactants, or any other surfactant capable of performing the aforementioned functions.

[0026] Here, the sampling needle delivers the blood of the animal to be tested into the first counting chamber component, and the reagent delivery component adds diluent and hemolysin to the first counting chamber component to obtain a blood sample, which is the test sample. The first counting chamber component then tests the test sample, i.e., the blood sample, in the first counting chamber component.

[0027] White blood cell counting and differential diagnosis can include three-part, four-part, and five-part differential methods. Taking three-part differential as an example, it refers to dividing white blood cells into three major groups using a dilution solution: small cell group (composed of lymphocytes), intermediate cell group (composed of monocytes), and large cell group (composed of granulocytes). This yields the number of lymphocytes, monocytes, and granulocytes in the blood sample. Five-part differential can directly separate white blood cells into neutrophils, lymphocytes, eosinophils, basophils, and monocytes using dilution and chemical staining or impedance methods.

[0028] The first counting cell assembly can perform white blood cell testing on animal blood samples using impedance counting. The process of performing impedance counting on the sample in the first counting cell assembly is as follows: A sampling needle draws a fixed amount of blood from the animal sample and injects it into the first counting cell assembly. This blood is then mixed with a fixed amount of diluent to form a diluted solution with a specific dilution ratio. A hemolysin is added, and after mixing, impedance counting is performed in the first counting cell assembly to obtain the white blood cell count results. Specifically, the sample is first diluted in the front cell of the first counting cell assembly to obtain the test sample. Then, under negative pressure, the sample flows from the front cell through the detection orifice into the rear cell of the first counting cell assembly. During the counting process, the diluted sample flows from the front cell into the rear cell, forming a stable flow field. Because a constant current source is applied between the positive and negative electrodes, a stable electric field is formed, such as... Figure 2 As shown, when blood cells pass through the detection orifice 142, a pulse signal is generated, which is used to classify and count particles.

[0029] Based on the principle of impedance analysis, after treatment with a hemolytic agent, red blood cells rupture. The volume of the ruptured red blood cell fragments does not affect the white blood cell count. Under the action of the hemolytic agent, white blood cells shrink in size; different volumes of white blood cells produce different pulse sizes when passing through the orifice, resulting in white blood cells with volumes ranging from 35 to 250 ferrite liters (fL). After hemolytic treatment, lymphocytes are located in the small cell region; granulocytes in the large cell region; and monocytes in the intermediate cell region. The animal blood cell analyzer calculates the percentage of each subpopulation based on the proportion of each subpopulation to the total count. Figure 3 The diagram in the middle shows the three differential classifications of white blood cells: GOHST represents red blood cell fragments, LYM represents lymphocytes, MON represents monocytes, and GRAN represents neutrophils.

[0030] In this embodiment of the invention, the first counting pool component does not limit the number of white blood cells classified in the test sample.

[0031] Of course, the embodiments of the present invention are not limited to providing methods and hardware, and can also be implemented in various ways, such as providing a storage medium (containing programs or instructions for executing the animal blood cell analysis method provided in the embodiments of the present invention).

[0032] Figure 4 This is a schematic diagram illustrating the implementation process of the animal blood cell analysis method provided in this embodiment of the invention, as shown below. Figure 4 As shown, it includes:

[0033] S401, Select a target measurement mode from multiple animal measurement modes, and determine the target dose of the diluent based on the target measurement mode.

[0034] The mode selection module in the animal blood cell analyzer provides multiple animal measurement modes, such as rat measurement mode, cat measurement mode, dog measurement mode, etc., or animal measurement mode 1, animal measurement mode 2, etc. In one example, one animal measurement mode corresponds to one species of animal; for example, the rat measurement mode corresponds to rats, the cat measurement mode corresponds to cats, and the dog measurement mode corresponds to dogs. In another example, one animal measurement mode corresponds to multiple species of animals; for example, animal measurement mode 1 corresponds to both mice and cats, and animal measurement mode 2 corresponds to dogs.

[0035] An animal measurement modality has a corresponding dose of diluent. The dose of diluent may be the same or different for different animal measurement modalities; that is, the dose of diluent may be the same or different for different animal species.

[0036] In practical applications, animal species with the same diluent dosage can correspond to the same animal measurement mode, while animal species with different diluent dosages correspond to different animal measurement modes. For example, the animal species include: species A, species B, species C, and species D. The diluent dosage for species A and C is dosage 1, the diluent dosage for species B is dosage 2, and the diluent dosage for species D is dosage 3. Therefore, the animal measurement mode for species A and C is animal measurement mode a, the animal measurement mode for species B is animal measurement mode b, and the animal measurement mode for species D is animal measurement mode c. Furthermore, the diluent dosage for animal measurement mode a is dosage 1, the diluent dosage for animal measurement mode b is dosage 2, and the diluent dosage for animal measurement mode c is dosage 3.

[0037] The mode selection module in the animal blood cell analyzer determines the animal measurement mode corresponding to the blood of the animal to be tested based on the user's operation or the scanning of the scanner in the animal blood cell analyzer, and uses the dose of the diluent corresponding to the determined animal measurement mode as the current target dose to determine the dose of diluent that needs to be injected into the first counting cell component.

[0038] S402. The target dose of diluent is mixed with the blood of the animal to be tested and hemolysis is performed to obtain the test sample.

[0039] After the mode selection module in the animal blood cell analyzer determines the target dose of the diluent, it sends the determined target calculation to the reagent delivery assembly. The reagent delivery assembly mixes the target dose of diluent with the blood of the animal to be tested and performs hemolysis to obtain the test sample. Here, the sampling needle assembly is used to draw blood from the animal to be tested and deliver it to the first counting cell assembly.

[0040] The reagent delivery assembly mixes the hemolytic agent with the blood of the animal to be tested to hemolyze the blood, dissolving the red blood cells in the blood while retaining the white blood cells.

[0041] The sampling needle assembly delivers the blood of the animal to be tested into the first counting chamber assembly, while the reagent delivery assembly adds the target dose of diluent and hemolysing agent to the first counting chamber assembly. The blood of the animal to be tested is diluted and hemolyzed in the first counting chamber assembly to obtain the test sample. Optionally, the reagent delivery assembly adds the target dose of diluent to the first counting chamber assembly all at once; alternatively, the reagent delivery assembly adds the target dose of diluent to the first counting chamber assembly in multiple portions.

[0042] In one example, the animal blood cell analyzer is provided with a diluent storage area for storing diluent and a hemolysin storage area for storing hemolysin. The diluent storage area and the hemolysin storage area are respectively provided with liquid channels connected to the first counting cell component. The reagent delivery component controls the flow of diluent from the diluent storage area and hemolysin from the hemolysin storage area to the first counting cell component by controlling the opening and closing of the corresponding liquid channels.

[0043] In one example, the animal blood cell analyzer is equipped with a sampling needle that draws a certain amount of blood from a test tube containing the blood of the animal to be tested. After the sampling needle draws the blood from the test tube under the control of a syringe, it moves to the detection area where the first counting chamber component is located. The syringe then injects the blood from the sampling needle into the first counting chamber component, thus adding the blood to the first counting chamber component. Alternatively, the sampling needle can first be moved above the first counting chamber component. Before the syringe injects the blood from the sampling needle into the first counting chamber component, the sampling needle can be moved vertically from above the first counting chamber component into the chamber of the first counting chamber component. The syringe then injects the blood from the sampling needle located in the chamber into the first counting chamber component, thus preventing the blood from splashing out of the first counting chamber component.

[0044] For different target measurement modes, the amount of animal sample drawn by the sampling needle can be the same or different. In practical applications, for the same animal blood cell analyzer, the sampling needle draws the same dose of animal sample from the test tube, that is, the dose of animal blood added to the first counting chamber component is the same, thus facilitating the comparison of test results for different species of animals.

[0045] In this embodiment of the invention, the reagent delivery assembly may include one or more syringes, and the syringe used to add the diluent and the hemolysin to the first counting chamber assembly may be a single syringe or different syringes. For example, the reagent delivery assembly includes a single syringe through which the diluent and the hemolysin are added to the first counting chamber assembly. Alternatively, the reagent delivery assembly may include a first syringe and a second syringe, where the first syringe is used to add the diluent to the first counting chamber assembly, and the second syringe is used to add the hemolysin to the first counting chamber assembly. This embodiment of the invention does not impose any limitations on the number of syringes included in the reagent delivery assembly or the objects to which the syringes are applied.

[0046] In this embodiment of the invention, the order in which the sampling needle assembly delivers the blood of the animal to be tested into the first counting chamber assembly, and the reagent delivery assembly adds the diluent and hemolysin to the first counting chamber assembly, is not limited. For example, the sampling needle assembly and the reagent delivery assembly sequentially add the diluent, the blood of the animal to be tested, and the hemolysin to the first counting chamber assembly. Another example: the sampling needle assembly and the reagent delivery assembly sequentially add the diluent, the hemolysin, and the blood of the animal to be tested to the first counting chamber assembly. Yet another example: the sampling needle assembly and the reagent delivery assembly simultaneously add the diluent and the blood of the animal to be tested to the first counting chamber assembly, and after adding the diluent and the blood of the animal to be tested, the reagent delivery assembly adds the hemolysin to the first counting chamber assembly.

[0047] In practical applications, a diluent is added at the same time as the blood of the animal to be tested. The diluent is used to clean the sampling needle that draws the blood of the animal to be tested, thereby ensuring that all the blood of the animal to be tested drawn by the sampling needle can be added to the first counting cell assembly.

[0048] In one embodiment, before mixing the target dose of diluent with the blood of the animal to be tested and performing hemolysis, the reagent delivery component of the animal blood cell analyzer empties the first counting chamber component, thereby removing all liquid from the first counting chamber component and preventing any residual liquid in the first counting chamber component from affecting the test results.

[0049] In one embodiment, during the process of mixing the target dose of diluent with the blood of the animal to be tested and performing hemolysis in the reagent delivery component of the animal blood cell analyzer, when the first counting cell component includes at least two of the diluent, the blood of the animal to be tested, and the hemolysing agent, the liquid in the first counting cell component is mixed to ensure that the diluent and the blood of the animal to be tested, and the hemolysing agent and the blood of the animal to be tested, react fully to ensure the accuracy of the test results.

[0050] In this embodiment of the invention, the dosage of hemolytic agent added by the reagent delivery component to the first counting cell component can be adjusted according to the animal measurement mode, and the hemolysis treatment time, i.e. the reaction time between the hemolytic agent and the blood of the animal to be tested, can also be adjusted according to the animal measurement mode.

[0051] S403. Test the sample to obtain white blood cell count and classification results.

[0052] The sampling needle assembly delivers the blood of the animal to be tested into the first counting chamber assembly, and the reagent delivery assembly adds the target dose of diluent and hemolysing agent to the first counting chamber assembly to obtain a diluted and hemolyzed test sample. The test results in the animal blood cell analyzer are used to test the test sample in the first counting chamber assembly to obtain the white blood cell count and classification results.

[0053] Optionally, after obtaining a test sample in the first counting cell component, the animal blood cell analyzer triggers the first counting cell component to test the test sample based on user operation. Alternatively, after obtaining a test sample in the first counting cell component, the animal blood cell analyzer automatically triggers the first counting cell component to test the test sample. In one example, the animal blood cell analyzer automatically triggers the first counting cell component to test the test sample based on the reaction time between the hemolysin and the blood of the animal being tested.

[0054] After obtaining the white blood cell count and classification results, the first counting chamber component can send these results to the display of the animal blood cell analyzer, where they are presented to the testing personnel. Alternatively, it can send the white blood cell count and classification results to the display of other devices besides the animal blood cell analyzer, where they are presented to the testing personnel. Here, the animal blood cell analyzer can be connected to other devices via wired, wireless, or other methods.

[0055] In this embodiment of the invention, a target dose of diluent for diluting animal blood is determined based on the animal measurement mode corresponding to the animal species. The target dose of diluent is used to dilute the blood of the animal to be tested. Thus, when testing the blood of the animal to be tested, the corresponding dose of diluent is used to dilute the blood of the animal to be tested for different species. By controlling the dose of diluent, the accuracy of the test results for white blood cells such as lymphocytes is improved.

[0056] In this embodiment of the invention, for different types of animals, the dilution and hemolysis treatment of the blood of the animal to be tested can be adjusted by adjusting the dosage of the diluent, the dosage of the hemolytic agent, and the reaction time between the hemolytic agent and the blood of the animal to be tested, so as to improve the accuracy of white blood cell classification and counting results of various animal blood.

[0057] based on Figure 1 The animal blood cell analyzer shown in the embodiment of the present invention can also be used as follows: Figure 5 As shown, the system includes a display 105 and an input device 106. The display 105 is used to display the software interface, and the input device 106 includes a keyboard or mouse, or other device for users to input information or commands. In this embodiment of the invention, the display 105 and the input device 106 can be integrated into a touch display, which can receive user input information or commands while displaying the software interface.

[0058] In one embodiment, based on Figure 5 The animal blood cell analyzer shown in section S401 selects a target measurement mode from multiple animal measurement modes, including: outputting an animal measurement mode selection interface; receiving an input operation or selection operation for an animal measurement mode in the measurement mode selection interface; and determining the target measurement mode from multiple animal measurement modes based on the input operation or the selection operation.

[0059] Here, the display 105 is used to output an animal measurement mode selection interface; the input device 106 is used to receive input operations or selection operations for animal measurement modes in the measurement mode selection interface; and the mode selection module 102 is used to determine a target measurement mode from multiple animal measurement modes based on the input operation or the selection operation.

[0060] The display can output an animal measurement mode selection interface for selecting animal measurement modes. The animal measurement mode can display a variety of animal measurement modes or animal species, allowing the user to input the species of the animal to be measured or the target measurement mode through the input device based on the animal measurement mode selection interface. When the user inputs the species of the animal, the mode selection module can determine the animal measurement mode corresponding to the input animal species as the target measurement mode from a variety of animal measurement modes.

[0061] The input device can receive user input or selection operations. When the input device receives user input, an input interface can be provided in the animal measurement mode selection interface to receive the animal species or target measurement mode input by the user. When the input device receives user input, the animal measurement mode selection interface can provide multiple animal species or animal measurement modes and determine the animal species or target measurement mode selected by the user.

[0062] based on Figure 1 The animal blood cell analyzer shown in the embodiment of the present invention can also be used as follows: Figure 5 As shown, it includes: scanner 107. Scanner 107 is used to scan information labels such as barcodes and QR codes to obtain the information carried by the information labels.

[0063] In one embodiment, based on Figure 5 The animal blood cell analyzer shown selects a target measurement mode from multiple animal measurement modes, including: scanning the information label on the test tube containing the blood of the animal to be tested to obtain the sample information of the blood of the animal to be tested; and selecting the target measurement mode from multiple animal measurement modes based on the sample information.

[0064] Here, the scanner 107 is used to scan the information markings on the test tube containing the blood of the animal to be tested to obtain the sample information of the blood of the animal to be tested; the mode selection module 102 is used to select the target measurement mode from multiple animal measurement modes according to the sample information.

[0065] The test tube containing the blood of the animal to be tested is labeled with a barcode, QR code, or other information carrying information about the blood. This information may include: the species of animal to which the blood belongs, the universally unique identifier (UUID) of the blood, and the sample information for the test items. In this embodiment of the invention, no limitations are placed on the sample information carried by the label.

[0066] When the test tube containing the blood of the animal to be tested is within the scanning range of the scanner, the scanner scans the information label on the test tube to obtain the sample information of the blood of the animal to be tested carried by the information label, and sends the scanned sample information of the blood of the animal to be tested to the mode selection module. The mode selection module determines the animal measurement mode corresponding to the blood of the animal to be tested in the test tube, i.e., the target measurement mode, based on the sample information related to the animal species or animal measurement mode in the sample information scanned by the scanner.

[0067] For example: If the sample information scanned by the scanner includes information representing the animal measurement pattern, and the animal measurement pattern is a feline measurement pattern, then the pattern selection module determines the target measurement pattern to be the feline measurement pattern. As another example: If the sample information scanned by the scanner includes the animal species to which the blood sample belongs, and the animal species is a cat, then the pattern selection module determines the target measurement pattern to be the feline measurement pattern. Yet another example: If the sample information scanned by the scanner includes the sample ID (UUID) of the blood sample to be tested, and the sample identifier is 1111, the pattern selection module determines the animal measurement pattern corresponding to the blood sample to be a feline measurement pattern based on the sample identifier 1111, thus determining the target measurement pattern to be the feline measurement pattern.

[0068] In this embodiment of the invention, depending on whether the target dose of diluent is added to the first counting chamber assembly all at once, the method of adding diluent, test animal blood, and hemolytic agent to the first counting chamber assembly may include:

[0069] Joining Method 1: Join all at once

[0070] In method one, S402 involves mixing the target dose of diluent with the blood of the animal to be tested and performing hemolysis to obtain a test sample. This includes: adding the target dose of diluent to the first counting chamber assembly; adding a hemolytic agent and the blood of the animal to be tested to the first counting chamber assembly to obtain a test sample. Here, the sampling needle assembly delivers the blood of the animal to be tested into the first counting chamber assembly, and the reagent delivery assembly adds the target dose of diluent to the first counting chamber assembly; and adds a hemolytic agent to the first counting chamber assembly.

[0071] When the reagent delivery assembly adds diluent to the first counting chamber assembly, it directly adds the target dose of diluent to the first counting chamber assembly. Following the addition of the target dose of diluent, a hemolytic agent is added to the first counting chamber assembly. The sampling needle can deliver the blood of the animal to be tested into the first counting chamber assembly during or after the reagent delivery assembly adds the diluent. For example: the reagent delivery assembly adds the target dose of diluent to the first counting chamber assembly, and after the reagent delivery assembly adds the target dose of diluent, the sampling needle assembly adds the blood of the animal to the first counting chamber assembly. After the sampling needle assembly adds the blood of the animal to the first counting chamber assembly, the reagent delivery assembly continues to add the hemolytic agent to the first counting chamber assembly. Another example: during the addition of the target dose of diluent to the first counting chamber assembly, the sampling needle assembly adds the blood of the animal to the first counting chamber assembly, and after the sampling needle assembly adds the blood of the animal to the first counting chamber assembly, the reagent delivery assembly continues to add the hemolytic agent to the first counting chamber assembly.

[0072] In this embodiment of the invention, when the target dose of diluent is added at once, the process of adding the diluent is uninterrupted, but there is no limitation on the order in which the diluent, hemolysin, and blood of the animal to be tested are added.

[0073] Method 2: Join in stages

[0074] In the first addition method, S402 mixes the target dose of diluent with the blood of the animal to be tested and performs hemolysis, including: adding the blood of the animal to be tested to a first dose of diluent to obtain a reference test sample; adding a second dose of diluent and hemolysin to the reference test sample to obtain the test sample, wherein the sum of the first dose and the second dose is the target dose. Here, the reagent delivery assembly adds a first dose of diluent to the first counting chamber assembly before or during the sampling needle assembly delivering the blood of the animal to be tested into the first counting chamber; and adds a second dose of diluent and the hemolysin to the first counting chamber assembly after the blood of the animal to be tested is added.

[0075] The reagent delivery assembly first adds a first dose of diluent to the first counting chamber assembly, and the sampling needle assembly adds the blood of the animal to be tested to the first counting chamber assembly. After the sampling needle assembly adds the blood of the animal to be tested to the first counting chamber assembly, the reagent delivery assembly continues to add a second dose of diluent to the first counting chamber assembly and adds a hemolysin, thus first adding a first dose of diluent to the first counting chamber assembly and then adding a second dose of diluent to the first counting chamber assembly.

[0076] Here, the addition of a first dose of diluent to the first counting chamber assembly by the reagent delivery assembly can be performed before or simultaneously with the addition of the animal blood to the first counting chamber assembly by the sampling needle assembly.

[0077] When the dosage of blood from the animal being tested is the same for different animal measurement modes, the initial dose can be the same or different.

[0078] In one embodiment, the animal blood cell analyzer aspirates a fixed dose of the reference test sample from the reference test sample before adding a second dose of diluent and hemolysin to the reference test sample; the fixed dose of the reference test sample is then subjected to red blood cell and / or platelet testing. Here, the sampling needle assembly in the animal blood cell analyzer aspirates a fixed dose of the reference test sample, consisting of the first dose of diluent and the blood of the animal to be tested, from the first counting chamber assembly; the second counting chamber assembly performs red blood cell and / or platelet testing on the fixed dose of the reference test sample.

[0079] The second counting chamber assembly is a different counting chamber assembly from the first counting chamber assembly. Optionally, the second counting chamber assembly and the first counting chamber assembly belong to the same animal blood cell analyzer. Optionally, the second counting chamber assembly and the first counting chamber assembly belong to different animal blood cell analyzers.

[0080] Before adding a hemolysin to the first counting chamber assembly to form a test sample, the first counting chamber assembly contains only a first dose of diluent and the blood of the animal to be tested. That is, the first counting chamber assembly contains a reference test sample diluted with the blood of the animal to be tested. At this time, the reference test sample can be drawn from the first counting chamber assembly by the sampling needle assembly, and the reference test sample drawn from the sampling needle by the second counting chamber assembly performs at least one of the following tests related to red blood cells: red blood cell test and platelet test.

[0081] The dose of the reference test sample drawn by the sampling needle assembly from the first counting cell assembly, i.e., the fixed dose, can be 9 μL, 11 μL, etc. The size of the fixed dose can be set according to actual needs, and this embodiment of the invention does not impose any limitation on it.

[0082] In one embodiment, the first dose is the same for different animal measurement patterns.

[0083] For different animal measurement modes, i.e. different animal species, if the dose of the test animal sample in the reference test sample is the same and the dose of the diluent is the same, the dilution ratio of the reference test sample corresponding to different animal species is the same, which facilitates the comparison of red blood cell or platelet test results of different animal species.

[0084] In addition method two, depending on whether the diluent of the first dose is added to the first counting cell component in one go, addition method two may include the following two cases:

[0085] Scenario 1: The first dose of diluent is added all at once.

[0086] In scenario one, adding the animal blood to the first dose of diluent to obtain a reference test sample includes: adding the first dose of diluent to the first counting chamber assembly; and adding the animal blood to the first counting chamber assembly to obtain a reference test sample. Here, the reagent delivery assembly adds the first dose of diluent to the first counting chamber assembly before the sampling needle assembly delivers the animal blood into the first counting chamber assembly.

[0087] Scenario 2: The first dose of diluent is added in portions.

[0088] In scenario two, adding the animal blood to the first dose of diluent to obtain a reference test sample includes: adding a third dose of diluent to the first counting chamber assembly; adding a fourth dose of diluent and the animal blood to the first counting chamber assembly to obtain a reference test sample, wherein the sum of the third dose and the fourth dose is the first dose. Here, the reagent delivery assembly adds a third dose of diluent to the first counting chamber assembly before the sampling needle assembly delivers the animal blood into the first counting chamber assembly; and adds a fourth dose of diluent and the animal blood into the first counting chamber assembly while the sampling needle assembly delivers the animal blood into the first counting chamber assembly.

[0089] It should be noted that Case 1 and Case 2 are examples of adding the target dose diluent to the first counting chamber component in two and three parts, respectively. In practical applications, the number of times the target dose diluent is added, as well as the order of adding the hemolysin and the blood to be tested, can be controlled according to actual needs.

[0090] The reagent delivery assembly and the sampling needle assembly can simultaneously add a fourth dose of diluent and the blood of the animal to be tested to the first counting chamber assembly. The added diluent washes the blood of the animal to be tested on the sampling needle, ensuring that all the blood of the animal to be tested can be added to the first counting chamber assembly, thus ensuring the accuracy of the classification and counting results.

[0091] The animal blood cell analysis method provided in this invention allows operators to flexibly set the diluent addition process according to actual needs. For example, when adding the target dose of diluent, the first dose of diluent and the blood of the animal to be tested are first added to the first counting chamber component, and then the second dose of diluent and hemolysin are added to the first counting chamber component. In this way, a reference test sample is obtained during the formation of the test sample, enabling the testing of red blood cells, platelets, and other red blood cell-related tests to be performed simultaneously with the white blood cell test.

[0092] The animal blood cell analysis method provided in this embodiment of the invention will be described below through specific test scenarios, such as... Figure 6 As shown, it includes:

[0093] S601, The sampling needle assembly draws blood from the animal to be tested.

[0094] The sampling needle assembly draws a specified dose of blood from the test tube from the animal to be tested.

[0095] S602, the first counting pool component is emptied.

[0096] S603, The reagent delivery assembly adds a first dose of diluent to the first counting cell assembly.

[0097] The syringe in the reagent delivery assembly opens the fluid passage between the first counting chamber assembly and the diluent storage location, adds diluent to the first counting chamber assembly through the opened passage, and closes the opened passage after adding the first dose of diluent. During this process, the fluid passage between the first counting chamber assembly and the hemolysin storage location remains closed.

[0098] S604. The sampling needle assembly adds the blood of the animal to be tested to the first counting cell assembly.

[0099] The syringe in the sampling needle assembly controls the sampling needle to move from the aspiration area for drawing blood from the test tube to the detection area for testing the blood sample above the first counting cell assembly, and from above the first counting cell assembly into the cell of the first counting cell assembly. The syringe in the sampling needle assembly injects the blood from the sampling needle located in the cell of the first counting cell assembly into the first counting cell assembly.

[0100] S605, mix well.

[0101] The diluent in the first counting cell assembly is mixed with the blood of the animal to be tested to obtain a reference test sample.

[0102] S606, The sampling needle assembly draws a reference test sample from the first counting cell assembly for red blood cell and platelet testing.

[0103] S607, The reagent delivery assembly adds a hemolytic agent to the first counting cell assembly.

[0104] The syringe in the reagent delivery assembly opens the fluid passage between the first counting chamber assembly and the hemolysin storage location, adds hemolysin into the first counting chamber assembly through the opened fluid passage, and closes the opened fluid passage after adding the hemolysin. During this process, the fluid passage between the first counting chamber assembly and the diluent storage location remains closed.

[0105] S608, The reagent delivery assembly adds a second dose of diluent to the first counting cell assembly.

[0106] The process of adding diluent to the first counting cell assembly by the reagent delivery assembly is the same as in S603.

[0107] S609, Mix well.

[0108] The diluent, the blood of the animal to be tested, and the hemolytic agent in the first counting cell assembly are mixed to obtain the test sample.

[0109] The S610 and the first counting pool component were tested.

[0110] The first counting pool component tests the reference sample in the first counting pool component to obtain white blood cell count and classification results.

[0111] The animal blood cell analysis method provided in this invention provides accurate results for lymphocyte testing while ensuring the accuracy of white blood cell counts. Different diluent volumes are used for different animal species, and the hemolysis dose and time are adjusted accordingly.

[0112] Before using the animal blood cell analysis method provided in this invention, the percentage of lymphocytes in mouse blood samples was 65%. Microscopic examination of these samples showed a lymphocyte percentage of 75%, a 10% difference between the microscopic result and the measured result. Our testing revealed that this problem existed in almost all samples. Adjusting the amount of different hemolysing agents and increasing the reaction time improved the lymphocyte count, but did not completely solve the problem of inaccurate lymphocyte counts. The animal blood cell analysis method provided in this invention, by changing the amount of diluent, can further improve the accuracy of lymphocyte test results.

[0113] The following describes the test results of the animal blood cell analysis method provided in this embodiment of the invention by using the lymphocyte count results in blood samples from different types of animals.

[0114] In this embodiment, the blood collection volume can be 9 μL. Before adjustment, all measurement modes correspond to 2.4 ml of diluent. Table 1 shows the blood collection from 7 horses to form 7 test animal blood samples, with the adjusted diluent volume set to 1.4 ml. The percentage of lymphocytes before and after dilution volume adjustment, as well as the percentage of lymphocytes in the microscopic examination results, are compared. Table 2 shows the blood collection from 7 mice to form 7 test animal blood samples, with the adjusted diluent volume set to 1.6 ml. The percentage of lymphocytes before and after dilution volume adjustment, as well as the percentage of lymphocytes in the microscopic examination results, are compared. Here, Lym% represents the percentage of lymphocytes.

[0115] Table 1

[0116]

[0117] Table 2

[0118]

[0119]

[0120] As can be seen from Tables 1 and 2, by adjusting the amount of blood sample diluent used in white blood cell counting for different animals, the accuracy of lymphocyte test results can be guaranteed while ensuring the accuracy of white blood cell count results.

[0121] This invention further provides a storage medium, namely a computer-readable storage medium, on which an executable program is stored. When the executable program is executed by a processor, it implements the steps of the animal blood cell analysis method performed by the above-mentioned animal blood cell analyzer.

[0122] The description of the above medium embodiments is similar to that of the above method embodiments, and has similar beneficial effects. For technical details not disclosed in the storage medium embodiments of the present invention, please refer to the description of the method embodiments of the present invention for understanding.

[0123] In this embodiment of the invention, if the above-described animal blood cell analysis method is implemented as a software functional module and sold or used as an independent product, it can also be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this embodiment of the invention, or the part that contributes to the prior art, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the methods described in the various embodiments of the invention. The aforementioned storage medium includes various media capable of storing program code, such as a USB flash drive, portable hard drive, read-only memory (ROM), magnetic disk, or optical disk. Thus, this embodiment of the invention is not limited to any specific hardware and software combination.

[0124] It should be understood that the phrase "one embodiment" or "an embodiment" throughout the specification means that a specific feature, structure, or characteristic related to the embodiment is included in at least one embodiment of the invention. Therefore, phrases such as "in one embodiment" or "in an alternative embodiment" appearing throughout the specification do not necessarily refer to the same embodiment. Furthermore, these specific features, structures, or characteristics can be combined in any suitable manner in one or more embodiments. It should be understood that in the various embodiments of the invention, the sequence numbers of the above-described processes do not imply a sequential order of execution; the execution order of each process should be determined by its function and internal logic, and should not constitute any limitation on the implementation process of the embodiments of the invention. The sequence numbers of the above-described embodiments of the invention are merely descriptive and do not represent the superiority or inferiority of the embodiments.

[0125] It should be noted that, in this document, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Unless otherwise specified, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes that element.

[0126] In the several embodiments provided by this invention, it should be understood that the disclosed devices and methods can be implemented in other ways. The device embodiments described above are merely illustrative. For example, the division of units is only a logical functional division, and in actual implementation, there may be other division methods, such as: multiple units or components can be combined, or integrated into another system, or some features can be ignored or not executed. In addition, the coupling, direct coupling, or communication connection between the various components shown or discussed can be through some interfaces, and the indirect coupling or communication connection between devices or units can be electrical, mechanical, or other forms.

[0127] The units described above as separate components may or may not be physically separate. The components shown as units may or may not be physical units. They may be located in one place or distributed across multiple network units. Some or all of the units may be selected to achieve the purpose of this embodiment according to actual needs.

[0128] In addition, in the various embodiments of the present invention, each functional unit can be integrated into one processing unit, or each unit can be a separate unit, or two or more units can be integrated into one unit; the integrated unit can be implemented in hardware or in the form of hardware plus software functional units.

[0129] Those skilled in the art will understand that all or part of the steps of the above method embodiments can be implemented by hardware related to program instructions. The aforementioned program can be stored in a computer-readable storage medium. When the program is executed, it performs the steps of the above method embodiments. The aforementioned storage medium includes various media that can store program code, such as mobile storage devices, read-only memory (ROM), magnetic disks, or optical disks.

[0130] Alternatively, if the integrated units of this invention are implemented as software functional modules and sold or used as independent products, they can also be stored in a computer-readable storage medium. Based on this understanding, the technical solutions of the embodiments of this invention, or the parts that contribute to the prior art, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the methods described in the various embodiments of this invention. The aforementioned storage medium includes various media capable of storing program code, such as mobile storage devices, ROMs, magnetic disks, or optical disks.

[0131] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A method for analyzing animal blood cells, applied to an animal blood cell analyzer, the method comprising: A target measurement mode is selected from multiple animal measurement modes, and the target dose of the diluent is determined based on the target measurement mode; wherein, the animal measurement mode is determined according to the animal species; different animal measurement modes correspond to different doses of diluent, while the same animal measurement mode corresponds to the same dose of diluent; for the same animal blood cell analyzer, different animal measurement modes correspond to the same dose of blood from the animal being tested; the animal measurement modes include: rat measurement mode, cat measurement mode, and dog measurement mode; Add the blood of the animal to be tested to the first dose of diluent to obtain a reference test sample; A fixed dose of the reference test sample is drawn from the reference test sample; Red blood cell and / or platelet tests were performed on the reference test sample at the fixed dose. A second dose of diluent and hemolytic agent is added to the reference test sample to obtain the test sample; the sum of the first dose and the second dose is the target dose; The test sample was tested to obtain white blood cell count and classification results.

2. The method according to claim 1, wherein, Select the target measurement mode from a variety of animal measurement modes, including: Output the animal measurement mode selection interface; Receive input or selection operations for the animal measurement mode in the measurement mode selection interface; The target measurement mode is determined from a variety of animal measurement modes based on the input operation or the selection operation.

3. The method according to claim 1, wherein, Select the target measurement mode from a variety of animal measurement modes, including: Scan the information label on the test tube containing the blood of the animal to be tested to obtain the sample information of the blood of the animal to be tested; Based on the sample information, a target measurement mode is selected from multiple animal measurement modes.

4. The method according to claim 1, wherein, The first dose was the same for different animal measurement models.

5. The method according to claim 1, wherein, The step of adding the blood of the animal to be tested to the first dose of diluent to obtain a reference test sample includes: A first dose of diluent is added to the first counting cell assembly; The blood of the animal to be tested is added to the first counting cell assembly to obtain a reference test sample.

6. The method according to claim 1, wherein, The step of adding the blood of the animal to be tested to the first dose of diluent to obtain a reference test sample includes: Add a third dose of diluent to the first counting cell assembly; A fourth dose of diluent and the blood of the animal to be tested are added to the first counting cell assembly to obtain a reference test sample. The sum of the third dose and the fourth dose is the first dose.

7. An animal blood cell analyzer, the animal blood cell analyzer comprising: A sampling needle assembly is used to draw blood from the animal to be tested and deliver it to the first counting chamber assembly; The mode selection module is used to select a target measurement mode from multiple animal measurement modes and determine the target dose of the diluent based on the target measurement mode; wherein, the animal measurement mode is determined according to the animal species; different animal measurement modes correspond to different doses of diluent, while the same animal measurement mode corresponds to the same dose of diluent; for the same animal blood cell analyzer, different animal measurement modes correspond to the same dose of blood from the animal being tested; the animal measurement modes include: rat measurement mode, cat measurement mode, and dog measurement mode; A reagent delivery assembly is used to add a first dose of diluent to the first counting chamber assembly before the sampling needle assembly delivers the blood of the animal to be tested into the first counting chamber assembly; The sampling needle assembly is also used to draw a fixed dose of a reference test sample consisting of the first dose of diluent and the blood of the animal to be tested from the first counting cell assembly and send it to the second counting cell assembly; The second counting cell assembly is used to perform red blood cell testing and / or platelet testing on the reference test sample at the fixed dose; The first counting pool component is used to test the test sample to obtain white blood cell count and classification results.

8. The animal blood cell analyzer according to claim 7, wherein, The animal blood cell analyzer also includes: a display and an input device; The display is used to output an animal measurement mode selection interface; The input device is used to receive input operations or selection operations for the animal measurement mode in the measurement mode selection interface. The mode selection module is used to determine the target measurement mode from multiple animal measurement modes based on the input operation or the selection operation.

9. The animal blood cell analyzer according to claim 7, wherein, The animal blood cell analyzer also includes: a scanner; The scanner is used to scan the information markings on the test tube containing the blood of the animal to be tested, and to obtain the sample information of the blood of the animal to be tested; The mode selection module is used to select a target measurement mode from multiple animal measurement modes based on the sample information.

10. The animal blood cell analyzer according to claim 7, wherein, The first dose was the same for different animal measurement models.

11. The animal blood cell analyzer according to claim 7, wherein, The reagent delivery assembly is also used for: Before the sampling needle assembly delivers the blood of the animal to be tested into the first counting chamber assembly, a third dose of diluent is added to the first counting chamber assembly; When the sampling needle assembly delivers the blood of the animal to be tested into the first counting chamber assembly, a fourth dose of diluent is added to the first counting chamber assembly, and the sum of the third dose and the fourth dose is the first dose.

12. A storage medium storing an executable program, which, when executed by a processor, performs the steps of the animal blood cell analysis method according to any one of claims 1 to 6.

Images