Automatic analysis device and automatic analysis method for a sample

Abstract

An automatic analysis device (100) is provided with an electrolyte measuring section (114) that performs measurement of an internal standard solution at least once before measurement of a potential of a sample, and in a case where measurement of the potential of the sample by the electrolyte measuring section (114) is continuously performed, the measurement operation of the internal standard solution before the measurement of the potential is changed depending on whether or not the sample measured the previous time is a high-concentration sample. Thus, an automatic analysis device and an automatic analysis method of a sample are provided, which can reduce carrying when performing electrolyte measurement, ensure measurement accuracy, and achieve improvement of the processing capacity of the entire device.

Description

Technical Field

[0001] This invention relates to an automated analytical apparatus and an automated analytical method for determining the concentration or activity of target components in biological samples such as blood or urine, and particularly to an automated analytical apparatus and an automated analytical method for samples equipped with an electrolyte analysis unit based on an ion-selective electrode. Background Technology

[0002] As an example of an automatic analytical device that balances the miniaturization of sample liquid volume and high measurement accuracy, Patent Document 1 describes the following: It includes an electrolyte sensor, a dilution tank with a monotonically inclined inner wall toward the deepest part of the point, a first tube for conveying sample solution from the dilution tank to the electrolyte sensor, and a second tube for discharging waste sample solution from the dilution tank to the outside, wherein one end of the first tube and one end of the second tube can be disposed near the deepest part of the dilution tank.

[0003] Existing technical documents

[0004] Patent documents

[0005] Patent Document 1: Japanese Patent Application Publication No. 2018-004388 Summary of the Invention

[0006] The problem that the invention aims to solve

[0007] An automated analysis device is a device used to analyze biological samples such as blood, urine, and bone marrow.

[0008] In such automated analytical apparatus, when determining the concentration of electrolytes (Na, K, Cl ions, etc.) in a sample, a representative method is to use a flow-type electrolyte concentration measuring device that utilizes an ion-selective electrode (ISE).

[0009] In clinical examinations using electrolyte concentration measuring devices, it is highly necessary to quantify the concentration of electrolytes in biological samples such as blood (especially serum and plasma) and urine, and the measurement data requires high precision.

[0010] One method for electrolyte determination is the dilution method. The dilution method has the advantage of requiring a small amount of sample solution, thus reducing sample consumption. In addition, it also offers several other advantages, such as lower concentrations of coexisting substances like proteins and lipids in the assay solution, less contamination from these coexisting substances, and higher stability of the electrolyte solution (ISE).

[0011] Therefore, in automated electrolyte analyzers, the combination of flow-through cell type ISE and dilution method has now become the mainstream.

[0012] In automated electrolyte analyzers, sample dilution is performed using a container called a dilution tank. The diluted sample prepared in the dilution tank is then piped to a flow cell type ISE for analysis. Internal standard solutions and samples are alternately dispensed into the dilution tank and analyzed alternately. The operations related to these measurements are performed according to a predetermined cycle time.

[0013] In ISE determinations, the potential of the sample solution is used not only for the sample but also for the internal standard solution. This is because the potential difference between the sample and the internal standard solution is typically used for concentration calculations.

[0014] In this way, in the determination of fluid electrolytes, the sample and the internal standard solution flow alternately within the flow path.

[0015] Here, when the electrolyte concentration of the sample is higher than that of the internal standard solution, the internal standard solution introduced into the flow path for the purpose of measurement will carry the previously measured sample components, and thus the potential of the internal standard solution may change from the value that should have been obtained.

[0016] That is, in subsequent sample measurements, if a variable internal standard solution is used, the potential difference between the sample and the internal standard solution will become smaller than it should be, resulting in a lower calculated sample concentration. In other words, due to this phenomenon, it may be impossible to obtain linearity in the measured values ​​in the high concentration region.

[0017] Suppressing carryover between the sample and the internal standard solution affects the reproducibility and measurable range of ISE, thus becoming a problem that needs to be solved to maintain and improve the performance of ISE measurement.

[0018] To address this issue, the technology described in Patent Document 1 above aims to determine the miniaturization and reduction of sample volume by using the shape of the ISE dilution tank and the configuration of each discharge nozzle and suction nozzle.

[0019] However, in the technology described in the aforementioned Patent Document 1, although the shape and configuration have been studied, there is room for achieving a stable reduction in residual liquid volume and more effectively reducing carryover due to deviation factors such as nozzle position adjustment and sample liquidity. In addition, there is room for preventing the accumulation of dirt in the case of long-term use of the dilution tank, which has become clear through the research of the inventors.

[0020] Here, we consider a method to reduce carryover by adding a wash between the sample determination and the internal standard solution. The wash in ISE is not only a detergent, but also involves the reagent being flowed into the dilution tank and flow path as a dummy and co-washed.

[0021] However, the potential of the internal standard solution before and after the sample measurement is generally used in the sample concentration calculation. Therefore, the following problem exists: if a cleaning process must be added due to the high concentration of the previous sample, the processing capacity will be reduced accordingly.

[0022] The purpose of this invention is to provide an automatic analysis device and an automatic analysis method for samples, which can reduce the carrying capacity while ensuring measurement accuracy when performing electrolyte determination, and can improve the overall processing capacity of the device.

[0023] Methods for solving problems

[0024] The present invention includes several methods for solving the above-mentioned problems. One example is an automatic analysis device for automatically performing sample analysis, characterized in that it includes an electrolyte measuring unit that performs an internal standard solution measurement at least once before the potential measurement of the sample. When the potential measurement of the sample performed by the electrolyte measuring unit is performed continuously, the measurement operation of the internal standard solution before the potential measurement is changed according to whether there is a possibility of a high concentration of the sample in the previous measurement.

[0025] Invention Effects

[0026] According to the present invention, when performing electrolyte measurements, it is possible to reduce the amount of material carried while ensuring measurement accuracy, and to improve the overall processing capacity of the device. Other issues, configurations, and effects beyond those described above will become clear through the following description of embodiments. Attached Figure Description

[0027] Figure 1 This is a diagram that schematically illustrates the overall configuration of an automatic analysis apparatus according to an embodiment of the present invention.

[0028] Figure 2 This is a schematic diagram showing the electrolyte measuring unit in the automatic analysis apparatus of an embodiment.

[0029] Figure 3 This is a flowchart illustrating the process of measuring electrolyte concentration in the electrolyte measuring unit of the automatic analysis apparatus according to an embodiment.

[0030] Figure 4 This is a schematic diagram showing the timing of the operation of the internal standard solution measurement and sample measurement during continuous measurement in the electrolyte measurement section of the automatic analysis apparatus of the embodiment.

[0031] Figure 5 This is a diagram illustrating a series of processing sequences for continuous measurement of internal standard solutions and sample measurements in the electrolyte measurement section of the automatic analysis apparatus of an embodiment.

[0032] Figure 6This is a diagram showing the timing sequence of two internal standard solutions measured in the electrolyte measurement section of the automatic analysis device according to an embodiment.

[0033] Figure 7 This is a diagram showing the timing sequence of other internal standard solutions measured in the electrolyte measurement section of the automatic analysis apparatus of an embodiment.

[0034] Figure 8 This is an example of an action setting screen for the internal standard solution timing in an automatic analysis device according to an embodiment.

[0035] Figure 9 This is a diagram showing the timing sequence of another internal standard solution measurement in the electrolyte measurement section of the automatic analysis device of an embodiment.

[0036] Figure 10 This is a diagram illustrating an example of a measurement mode in which the timing of measuring A using an internal standard solution is used in the analysis of the electrolyte measurement unit of the automatic analysis apparatus of an embodiment.

[0037] Figure 11 This is a diagram illustrating an example of a measurement mode in which the timing of measuring B using an internal standard solution is used in the analysis of the electrolyte measurement unit of the automatic analysis apparatus of an embodiment.

[0038] Figure 12 This is a flowchart illustrating the selection of action plans in the automatic analysis device of this embodiment.

[0039] Figure 13 This is an example of a screen showing the selection and setting of the internal standard solution timing in an automatic analysis device according to an embodiment.

[0040] Figure 14 This is another example of a screen showing the selection and setting of the internal standard solution timing in the automatic analysis device of an embodiment. Detailed Implementation

[0041] use Figures 1 to 14 The embodiments of the automatic analysis apparatus and the automatic analysis method for the sample of the present invention will be described below.

[0042] First, use Figure 1 The overall structure and operation of the automatic analysis device in this embodiment will be described. Figure 1 This is a diagram that schematically illustrates the overall configuration of the automatic analysis device in this embodiment.

[0043] Figure 1 The automatic analysis device 100 shown includes a conveying unit 101, an analysis unit 111, and an operation unit 130.

[0044] The transfer unit 101 is a unit used to load, retrieve, and transfer samples from a sample rack 104, which contains one or more sample containers holding biological samples such as blood or urine as the subjects of analysis, into the automatic analysis device 100 and to supply samples to the analysis unit 111.

[0045] The conveying unit 101 includes a support buffer 103, a support supply tray 102, a support storage tray 107, and a conveying line 106.

[0046] In the transport unit 101, the sample holder 104, mounted on the stent supply tray 102, is transported to the stent buffer 103 via the transport line 106. Midway along the transport line 106, a sample presence / absence determination sensor (not shown) identifies the presence or absence of a sample container on the sample holder 104. If a sample container is detected, the sample barcode (not shown) affixed to the sample container is read by a sample barcode reader (not shown) to identify the sample's identification information. In the actual automated analysis device 100, this identification information is used to determine the patient.

[0047] The support buffer 103 is a rotor structure that performs circular motion. It has slots on its outer circumference that radially hold multiple sample holders 104, each holding a sample container. The slots are configured to rotate via a motor, thereby moving any sample holder 104 into and out of the analytical unit 111 at its destination. This structure also eliminates the need to process previously placed sample holders 104 sequentially. That is, samples with higher priority can be processed first.

[0048] The transport line 106 is connected to a point on the radial circumference of the support buffer 103 to transport the sample holder 104 in and out. If this point is set as the 0-degree position on the circumference, the sample dispensing line 112, which is used to introduce the sample holder 104 to the analysis unit 111 described later, is connected at a 90-degree position on the circumference from the position where the transport line 106 is connected to the sample dispensing line 112 to transport the sample holder 104 in and out.

[0049] After the sample holder 104 has finished dispensing in the analysis unit 111, it can wait for the output of the measurement results in the holder buffer 103 and perform automatic re-inspection and other processing as needed. Alternatively, after processing is completed, it is transferred to the holder storage tray 107 via the transfer line 106.

[0050] The analysis unit 111 is a unit that performs the measurement actions of the test items entrusted to the sample and outputs the measurement results, and is connected to the transport unit 101.

[0051] The analysis unit 111 includes a reaction plate 115, a reagent plate 117, a sample dispensing line 112, a reagent dispensing nozzle 116, a sample dispensing nozzle 2, a colorimetric measuring section 118, and an electrolyte measuring section 114.

[0052] Reaction containers (not shown) are arranged on the circumference of the reaction disk 115. Near the reaction disk 115, there is a sample dispensing line 112 for loading and unloading sample containers onto a sample holder 104.

[0053] A rotatable and vertically movable sample dispensing nozzle 2 is provided between the reaction plate 115 and the sample dispensing line 112. The sample dispensing nozzle 2 moves in an arc around the rotation axis, dispensing the sample from the sample holder 104 to the dilution tank 1 in the reaction vessel on the reaction plate 115 or the electrolyte measuring section 114 (see reference). Figure 2 ) notes.

[0054] The reagent tray 117 is a storage container capable of holding multiple reagent bottles (not shown) containing reagents on its circumference. The reagent tray 117 is kept cold.

[0055] A reagent dispensing nozzle 116, capable of rotation and vertical movement, is provided between the reaction plate 115 and the reagent plate 117. The reagent dispensing nozzle 116 moves in an arc around the rotation axis, entering the reagent plate 117 from the suction port of the reagent dispensing nozzle to dispense the reagent from the reagent bottle to the reaction container.

[0056] Furthermore, cleaning tanks (not shown) are respectively provided within the operating range of reagent dispensing nozzle 116 and sample dispensing nozzle 2.

[0057] Electrolyte measuring unit 114 and colorimetric measuring unit 118 are arranged around reaction plate 115.

[0058] Electrolyte measuring unit 114 is an analytical unit that uses an ion-selective electrode to measure the concentration of electrolytes in a sample. Detailed information can be found using... Figure 2 To be described later.

[0059] The colorimetric measurement unit 118 is an analytical unit that analyzes the biochemical components in a sample by measuring the absorbance of the reaction liquid generated by mixing and reacting in the reaction vessel on the reaction plate 115. It performs analytical items with a different measurement principle than the electrolyte measurement unit 114. The colorimetric measurement unit 118 consists of a light source, a spectrophotometer, etc.

[0060] The operation unit 130 is responsible for providing information about the entire automatic analysis device 100, and includes a display unit 131, an input unit 132, a recording unit 133, and an overall control unit 134. The operation unit 130 is connected to the analysis unit 111 and the transfer unit 101 via a wired or wireless network line.

[0061] The display unit 131 is a part that displays various screens such as the operation screen for commanding the measurement of the sample to be measured, and the screen for confirming the measurement results. It is composed of an LCD or the like. It should be noted that it does not have to be an LCD or the like. It can be replaced by a printer or the like. It can also be composed of a display and a printer or the like. It can also be a touch panel type display that also includes the input unit 132 described later.

[0062] The input unit 132 is used to input various parameters, settings, measurement results, measurement commission information, analysis start and stop instructions, etc., based on the operation screen displayed on the display unit 131. It consists of a keyboard, mouse, etc.

[0063] The recording unit 133 is a part that stores timing diagrams, operation parameters, various information for determining biological samples, measurement results, etc., required for the operation of each device constituting the automatic analysis device 100. It is composed of storage media such as semiconductor memory including flash memory and disks including HDD.

[0064] The overall control unit 134 is the part that controls the operation of the automatic analysis device 100 as a whole, and includes a transport unit control unit 134a, an analysis unit control unit 134b, and a calculation and recording unit 134c.

[0065] The transfer unit uses a control unit 134a to control the transfer of the appropriate sample holder 104 from the support buffer 103 to the sample dispensing line 112 and the transfer of the sample holder 104 back from the sample dispensing line 112 to the support buffer 103.

[0066] The analysis unit control unit 134b is connected to each device in the analysis unit 111 and controls the analysis operation of each constituent device of the electrolyte measuring unit 114 and the colorimetric measuring unit 118.

[0067] The calculation and recording unit 134c calculates the concentration of a specific component in the test object based on the absorbance and other measurements measured in the colorimetric measurement unit 118, and calculates the ion concentration of the test object based on the potential and other measurements measured in the electrolyte measurement unit 114.

[0068] The transfer unit control unit 134a, the analysis unit control unit 134b, and the calculation and recording unit 134c within the overall control unit 134 can be implemented using a general-purpose computer, or they can be implemented as functions of a program executed on a computer.

[0069] That is, the processing of these transfer unit control unit 134a, analysis unit control unit 134b and calculation record unit 134c can also be stored as program code in a recording unit such as memory, and implemented by a processor such as CPU (Central Processing Unit) executing each program code.

[0070] It should be noted that the control unit 134a for the conveying unit, the control unit 134b for the analysis unit, and the calculation and recording unit 134c can also be constructed from dedicated circuit boards or other hardware.

[0071] In this embodiment, the case where the analytical unit provided simultaneously with the electrolyte measurement unit 114 is a colorimetric measurement unit 118 has been described. However, the analytical unit is not limited to a colorimetric measurement unit for measuring biochemical items. For example, a measurement unit for measuring immunological items and the electrolyte measurement unit 114 can be arranged in the same analytical unit. Alternatively, the analytical unit may be composed solely of the electrolyte measurement unit 114.

[0072] Furthermore, the case where the automatic analysis device 100 has one analysis unit 111 has been described, but it can have two or more analysis units. In this case, there is no particular limitation on the type of analysis unit, and it can have one or more biochemical analysis units, immunoassay units, blood coagulation analysis units, and other types of analysis units.

[0073] Furthermore, the case where the automatic analysis device 100 includes a transfer unit 101 is described, but the transfer unit is not necessary, and the automatic analysis device can be composed of an analysis unit and an operation unit.

[0074] Next, the explanation Figure 1 A summary of the mechanism operation of the automatic analysis device 100 shown.

[0075] The transfer unit 101 delivers the sample racks 104 from the support supply tray 102 of the automatic analysis device 100 to the transfer line 106 one rack at a time, and then moves them into the support buffer 103. The sample racks 104 delivered to the support buffer 103 are then transferred to the sample dispensing line 112 of the analysis unit 111.

[0076] When the sample holder 104 reaches the sample dispensing line 112, the sample dispensing operation is performed on each sample mounted on the sample holder 104 through the sample dispensing nozzle 2 according to the measurement items entrusted by the operation unit 130.

[0077] When the test item is a biochemical test, the sample dispensing nozzle 2 dispenses the sample into the reaction container located on the reaction plate 115. Reagent, drawn from the reagent plate 117 by the reagent dispensing nozzle 116, is then added to the reaction container, and the mixture is stirred. The absorbance is then measured by the colorimetric measuring unit 118, and the measurement result is sent to the calculation and recording unit 134c of the operation unit 130.

[0078] When the commissioned test is for an electrolyte test, the sample dispensing nozzle 2 draws the sample and discharges it into the dilution tank 1 of the electrolyte measurement unit 114. The electromotive force is measured by the ion-selective electrodes 7, 8, and 9, and the measurement result is sent to the calculation and recording unit 134c of the operation unit 130. However, in the case of an electrolyte test, as described above, a pre-testing action is required before sample dispensing to measure the electromotive force of an internal standard solution of known concentration.

[0079] The calculation and recording unit 134c of the operation unit 130 calculates the concentration of a specific component in the sample through calculation based on the sent measurement results. The analysis results are notified to the user via the display unit 131 and recorded in the recording unit 133.

[0080] Next, use Figure 2 An overview of the electrolyte measurement unit that uses an ion-selective electrode is provided. Figure 2 This is a schematic diagram showing an example of an electrolyte measuring unit using an ion-selective electrode.

[0081] The electrolyte measuring unit 114 is disposed in the analysis unit 111 that automatically performs sample analysis.

[0082] The electrolyte measuring unit 114 includes a dilution tank 1, a dilution solution dispensing nozzle 3, an internal standard solution dispensing nozzle 4, a sample solution suction nozzle 5, piping 6, a sodium ion selective electrode 7, a potassium ion selective electrode 8, a chloride ion selective electrode 9, a reference electrode 10, piping 11, a sample pump 12, a potential measuring unit 13, and a temperature adjustment unit 16.

[0083] Sample dispensing nozzle 2 dispenses blood, urine, and other samples into dilution tank 1, while diluent dispensing nozzle 3 dispenses diluent into dilution tank 1. Internal standard solution dispensing nozzle 4 dispenses internal standard solution into dilution tank 1.

[0084] Diluent is supplied from diluent container 14 to diluent dispensing nozzle 3 using a diluent pump (DIL pump) 18. Internal standard solution is supplied from internal standard solution container 15 to internal standard solution dispensing nozzle 4 using an internal standard solution pump (IS pump) 19.

[0085] The temperature adjustment unit 16 is configured in the flow path of the diluent and the internal standard solution, and adjusts the temperature to a constant temperature (e.g., 37°C) midway through the delivery of each solution. The flow path near the temperature adjustment unit 16 can also improve the temperature adjustment efficiency by making the flow path volume larger than other parts.

[0086] The sample liquid suction nozzle 5 is configured to move up and down, and the solution in the dilution tank 1 is drawn in by the driving force of the sample pump 12. The drawn solution is introduced into the flow path of the ion selective electrodes 7, 8, and 9 through the piping 6, and then disposed of as liquid through the piping 11.

[0087] In the electrolyte measurement unit 114, a sample introduction unit for introducing a sample solution containing electrolyte is used, comprising a sample solution pipette 5, tubing 6, tubing 11, and a sample pump 12. The sample solution is introduced into the flow paths of the ion-selective electrodes 7, 8, and 9 using this sample introduction unit.

[0088] Then, reference electrode solution is introduced from reference electrode solution container 17 to reference electrode 10 through piping 11 and sampling pump 12. In order to prevent contamination with the internal standard solution, valves or the like are used to switch the reference electrode solution to a different flow path than the internal standard solution in ion selective electrodes 7, 8, 9, etc.

[0089] The terminals of each ion-selective electrode 7, 8, 9 and the reference electrode 10 are connected to the potential measurement unit 13, and the potential difference between the electrodes is measured when the sample solution is introduced.

[0090] As described above, in the sample potential measurement operation in the electrolyte measurement unit 114, it is necessary to measure the internal standard solution. However, in this embodiment, when the sample potential measurement is continuously performed by the electrolyte measurement unit 114, the measurement operation of the internal standard solution before the potential measurement is changed according to whether the sample measured in the previous measurement may be a high-concentration sample.

[0091] In particular, in this embodiment, a first timing diagram and a second timing diagram are provided. The first timing diagram is for the case where the previous measurement could not have been of a high-concentration sample, and an idle time period is provided at the beginning of the timing diagram during which the electrolyte measuring unit 114 is not in operation. The second timing diagram is for the case where the previous measurement could have been of a high-concentration sample, and an idle time period is not provided at the beginning of the timing diagram during which the electrolyte measuring unit 114 is not in operation, and an action is performed to discharge or aspirate at least one of the internal standard solution and the diluent for diluting the sample.

[0092] In other words, when the electrolyte measuring unit 114 measures the sample in this embodiment, in the first timing diagram where the previous measurement could not have been a high-concentration sample, the action of discharging or aspirating at least one of the internal standard solution and the diluent of the diluted sample, which was performed when the previous measurement could have been a high-concentration sample, is not performed. The measurement of the internal standard solution begins after the time required for these actions has elapsed.

[0093] In contrast, in the second timeline where the previous measurement may have been of a high-concentration sample, at a time before the measurement of the internal standard solution, which corresponds to the idle time in the first timeline, an action is taken to drain or aspirate at least one of the internal standard solution and the diluent of the diluted sample.

[0094] The timing diagram controlling these actions is stored in the recording unit 133 and executed by the analysis unit of the overall control unit 134 using the control unit 134b.

[0095] Figure 3 It was used Figure 2 An example of a flowchart for electrolyte concentration measurement using an electrolyte concentration measuring device.

[0096] like Figure 3 As shown, firstly, the internal standard solution is discharged into the dilution tank 1 using the internal standard solution dispensing nozzle 4 (step S101).

[0097] Next, the internal standard solution in the dilution tank 1 is aspirated using the sample solution nozzle 5 and the sample pump 12 (step S102). As a result, the flow paths of the ion-selective electrodes 7, 8, and 9 are filled with the internal standard solution.

[0098] Next, the potentials of the ion-selective electrodes 7, 8, and 9, with reference electrode 10, are measured using the potential measurement unit 13 (step S103). The potentials of the ion-selective electrodes 7, 8, and 9 here are set to E1.

[0099] Next, the sample is discharged into the dilution tank 1 using the sample dispensing nozzle 2 (step S104).

[0100] Next, the diluent is dispensed into the dilution tank 1 using the diluent dispensing nozzle 3 (step S105). Thus, the sample is diluted in a predetermined ratio of sample volume to diluent volume.

[0101] Next, the diluted sample in the dilution tank 1 is aspirated using the sample liquid suction nozzle 5 and the sample pump 12 (step S106). As a result, the flow paths of the ion-selective electrodes 7, 8, and 9 are filled with the sample liquid.

[0102] Next, the potentials of ion-selective electrodes 7, 8, and 9, with reference electrode as the reference, are measured using the potential measurement unit (step S107). The potentials of ion-selective electrodes 7, 8, and 9 here are set to E2.

[0103] Next, the concentration of the target ion in the sample is calculated in the calculation and recording unit 134c based on the previously measured potentials E1 and E2 (step S108), and output to the recording unit 133, display unit 131, etc. (step S109).

[0104] Then, the internal standard solution is discharged into the dilution tank 1 again (step S101) and the internal standard solution is drawn into the dilution tank 1 again (step S102), and the measurement ends.

[0105] In the case of the first timeline in which the electrolyte determination is carried out continuously, steps S101 to S109 are repeated.

[0106] Figure 4 This is a summary of the timing diagrams for the internal standard solution measurement and sample measurement during continuous testing.

[0107] like Figure 4 As shown, firstly, in the determination of the internal standard solution, the internal standard solution is aspirated by the pump 19 to prepare for discharge into the dilution tank 1 (step S201). The aspiration volume at this time is set by the automatic analysis device 100, for example, 355 [μL].

[0108] Next, the diluent is aspirated by pump 18 to prepare for discharge into dilution tank 1 (step S202). The aspiration volume is also set by the automatic analysis device 100, for example, 330 [μL].

[0109] Then, a specified amount of internal standard solution and diluent is discharged (steps S203 and S204). The discharge volume is also set by the automatic analysis device 100, for example, 220 μL of internal standard solution and 330 μL of diluent are discharged.

[0110] Then, to prevent dripping from the nozzle tip, both the diluent dispensing nozzle 3 and the internal standard solution dispensing nozzle 4 draw in air (steps S205 and S206). This completes the cleaning process of the dilution tank before measuring the internal standard solution.

[0111] Next, the internal standard solution is drawn in by the internal standard solution pump 19 (step S207). The amount drawn in at this time is also set by the automatic analysis device 100, for example, 365 [μL]. Then, the internal standard solution is discharged into the dilution tank 1 by the internal standard solution pump 19 (step S208). The amount drawn in at this time is also set by the automatic analysis device 100, for example, 500 [μL]. After the internal standard solution is discharged, in order to prevent droplets from the nozzle, air is drawn from the internal standard solution dispensing nozzle 4 by the internal standard solution pump 19 (step S209).

[0112] Next, the internal standard solution discharged into the dilution tank 1 is introduced into the flow path of the ion-selective electrodes 7, 8, and 9 using the sample pump 12 (step S210). Afterward, to prevent dripping, air is drawn from the sample solution nozzle 5 using the sample pump 12 (step S211).

[0113] Next, in order to measure the potential of the reference electrode solution, the reference electrode solution is aspirated using the sampling pump 12 (step S212). Here, in order to prevent the reference electrode solution from contaminating the internal standard solution, a valve or the like is used to switch the flow path to a different one than that of the internal standard solution. As a result, the reference electrode 10 is filled with the reference electrode solution.

[0114] After a series of actions are completed, the sampling pump 12 is returned to its initial position to discard the liquid in the flow path (step S213). Finally, the potentials of the ion-selective electrodes 7, 8, and 9 and the reference electrode 10 are obtained (step S214).

[0115] Next, use the same method. Figure 4 The timeline diagram for sample measurement is explained.

[0116] In the sample determination, the first step is to perform a suction action for discharging the diluent by the diluent pump 18 (step S215).

[0117] Next, the diluent and the sample are discharged into the dilution tank 1. The diluent is discharged in two stages. After the first discharge of the diluent (step S216), the sample is discharged, followed by the second discharge of the diluent (step S217). The sample is discharged using the sample dispensing nozzle 2. The ratio of sample to diluent is set by the device. Then, to prevent dripping, air is drawn in by the diluent dispensing nozzle 3 (step S218).

[0118] Next, the sample diluent in the dilution tank 1 is drawn in by the sample pump 12 (step S219). After that, in order to prevent dripping, air is drawn in by the sample liquid suction nozzle 5 (step S220).

[0119] Next, in order to measure the potential of the reference electrode liquid, the sampling pump 12 is used to aspirate the reference electrode liquid (step S221). After a series of actions are completed, the sampling pump 12 is returned to its initial position to discard the liquid in the flow path of each electrode (step S222).

[0120] Finally, the potential of each electrode is obtained (step S223).

[0121] Here, as Figure 4 and Figure 5 As shown, the timing sequence of the above-mentioned internal standard solution determination and sample determination can be overlapped by adjusting the timing of the actions of each mechanism.

[0122] For example, such as Figure 5 As shown, in the cycle time T101 of the internal standard solution determination and the sample determination for a certain sample, the latter half of the internal standard solution determination is overlapped with the first half of the sample determination. Therefore, the sample determination can be performed in a total of T101 cycles, instead of T101×2 cycles.

[0123] With the electrolyte processing capacity of the device being 150 samples per hour, the operating cycle of T101 is 24 seconds. That is, the time spent on sample measurement is also 24 seconds. Similarly, the time spent on potential measurement of the internal standard solution is also 24 seconds.

[0124] In this embodiment, when continuously measuring the electrolyte concentration in the electrolyte measuring unit 114, the operation timing is used separately for cases where the previous measurement may have been of a high concentration sample and cases where such a high concentration sample was unlikely. Figure 6 The following diagrams will explain its details. Figure 6 The sequence of internal standard solution determination with a cleaning effect, implemented as a method for determining internal standard solutions, is shown after the potential measurement of high-concentration samples, and the sequence of internal standard solution determination is shown.

[0125] Figure 6 The determination sequence of the internal standard solution A shown in the upper paragraph is consistent with... Figure 4 The sequence of actions shown for the internal standard solution determination is the same as the sequence of actions for the previous determination, which is the determination of the potential of a sample in a general concentration range where a high concentration sample could not be measured (first sequence diagram).

[0126] In contrast, Figure 6 The sequence of measurement for internal standard solution B shown in the lower section is the same as the sequence of measurement in the case where the previous measurement may have involved a high concentration sample (second sequence diagram). A diluent suction and discharge action is added to the initial part of one cycle. Details of this action are described later.

[0127] By preparing a timing sequence that incorporates actions with such a cleaning effect into the timing sequence of internal standard solution determination, and using it separately from the usual timing sequence, the cleaning effect can be obtained without consuming cycles for cleaning at unnecessary times during continuous determination.

[0128] like Figure 6 As shown, before the actual start of the internal standard solution measurement process, the internal standard solution measurement A ensures an idle time as indicated by interval IS-1. For example, relative to the 24-second cycle of the internal standard solution operation, 5% to 20% of the time, such as 4.4 seconds (approximately 18.3%), is ensured as interval IS-1. This interval IS-1 is preferably the time required to complete the cleaning operation described later.

[0129] In contrast, the internal standard solution determination B uses the aforementioned interval IS-1, only attracting and discharging the diluent used as cleaning solution to introduce it into the electrode flow path. That is, interval IS-1 is used to perform the diluent attraction action (step S401) for discharging the diluent into the dilution tank 1, the diluent discharge action (step S402) for discharging the diluent into the dilution tank 1, and the air attraction action (step S403) for preventing dripping from the diluent dispensing nozzle 3. These actions are equivalent to conditioning actions and achieve the same effect on analytical performance.

[0130] The amount of diluent discharged here is preferably greater than that discharged in any of the steps S203, S204, and S208. For example, 600 μL is discharged here.

[0131] Furthermore, the time sequence diagrams that impart cleaning effects are not limited to... Figure 6 The internal standard solution for determination B shown can also be prepared in Figure 7 In the lower segment shown, during the IS-1 interval, in addition to the diluent, the internal standard solution is discharged and the internal standard solution is drawn for measurement C (second time series diagram). Figure 7 This is another example of the timing sequence for internal standard solution determination.

[0132] In the internal standard solution determination C, the internal standard solution suction action for discharging the internal standard solution into the dilution tank 1 (step S501) and the internal standard solution discharge action for discharging the internal standard solution into the dilution tank 1 (step S502) and the air suction action for preventing droplets from the internal standard solution dispensing nozzle 4 are implemented in parallel with the above steps S401, S402 and S403 using interval IS-1 (step S503).

[0133] Regarding the discharge volume of the diluent and the internal standard solution in the interval IS-1 of the internal standard solution determination C, it is preferable that the total discharge volume of the two liquids is greater than any one of the actions in steps S203, S204, and S208. For example, by setting the discharge volume of the diluent to 300 [μL] and the discharge volume of the internal standard solution to 300 [μL], the consumption volumes of the diluent and the internal standard solution can be made consistent.

[0134] Therefore, the temperature of the internal standard liquid flow path and nozzle can be adjusted, which can improve the stability when the internal standard liquid is discharged in steps S203 and S208.

[0135] It should be noted that the discharge ratio of the diluent and the internal standard solution can be changed according to the capacity of their respective reagent containers. For example, in a sequence such as when the internal standard solution is measured (C), a cleaning operation is performed using a mixture of the internal standard solution and the diluent, and the display unit 131 can display... Figure 8 As shown in the setting screen 601, the user can freely set the set discharge volume of the diluent in the diluent discharge volume selection area 602, and freely set the setting and output of the internal standard liquid in the internal standard liquid discharge volume selection area 603.

[0136] Generally speaking, regarding cleaning, as long as the sample components can flow out of the electrode flow path, the mixing ratio of the internal standard solution and diluent does not affect the cleaning performance. Furthermore, the diluent and internal standard solution are reagents used in actual sample measurements; therefore, even if the mixing ratio of the reagents changes during cleaning, it will not significantly affect the potential measurement of the sample.

[0137] In this way, by being able to set the amount of internal standard solution and diluent used during the cleaning process, a balance of consumption can be achieved, and the advantage of being able to replace reagent containers at the same time of the cycle can be obtained.

[0138] Furthermore, it is possible to prepare a timing diagram for introducing the diluent discharged into the dilution tank 1 and the internal standard solution into the electrode flow path in steps S402 and S502 above. A diagram illustrating the measurement timing of the internal standard solution under this condition is shown below. Figure 9 .

[0139] like Figure 9 As shown, in the measurement sequence of the internal standard solution D introduced to the electrode flow path side, the suction to the electrode flow path side and liquid waste need to be completed using interval IS-1. Therefore, a diluent suction action (step S404) for discharging diluent to diluent 1, a diluent discharge action (step S405) for discharging diluent to diluent 1, and an air suction action (step S406) for preventing dripping from diluent dispensing nozzle 3 are implemented. However, the time of these actions is respectively longer than... Figure 6 The steps S401, S402, and S403 shown are short.

[0140] Then, the diluent is drawn out into the dilution tank 1 using the sampling pump 12 (step S601). After that, to prevent dripping, air is drawn from the sample liquid nozzle 5 using the sampling pump 12 (step S602). After the series of actions are completed, the sampling pump 12 is returned to its initial position to discard the liquid in the flow path (step S603).

[0141] The aforementioned suction and liquid waste actions to the electrode flow path can be performed in the internal standard solution determination C described above and the internal standard solution determination E described later.

[0142] Moreover, although not illustrated, it is possible to prepare a timing diagram for the discharge and aspiration of the internal standard solution within the time interval IS-1, and in this case, the internal standard solution can also be introduced to the electrode flow path side.

[0143] Figure 10 An example of a measurement mode is shown where only samples in the general concentration range are measured in an ISE assay. Figure 11 An example of a measurement mode is shown, in which a high-concentration sample is measured during a sample measurement in a general concentration region.

[0144] In the case of measuring only samples in the general concentration range, such as Figure 10 As shown, only internal standard solution determination A is performed during the intervals between sample determinations. On the other hand, in the case of determining samples in high-concentration areas, as... Figure 11 As shown, the internal standard solution determination after potential measurement of the sample in the high concentration area is selected for internal standard solution determination B.

[0145] Here, the determination of whether the target sample is likely to be a sample from a high-concentration area is performed by setting the device.

[0146] For example, the settings used for identification utilize information set for each sample category.

[0147] Typically, when the device accepts a measurement request based on the master communication unit or manual input from the user, it includes a sample category setting. Here, sample category refers to the type of measurement sample collected for in vitro diagnostics, depending on the collection site. Examples include serum, plasma, urine, and bone marrow fluid.

[0148] The analysis unit of the overall control unit 134 uses control unit 134b to determine whether the sample meets the criteria for a high-concentration region sample based on the sample category information. For example, the high-concentration region of potassium ion concentration measurement range for urine samples is more than 10 times larger than that for typical serum samples; therefore, the urine sample is classified as a high-concentration region sample. The selection process for internal standard solution measurement corresponding to the sample type is shown below. Figure 12 .

[0149] like Figure 10 and Figure 11 As shown, for example, in the case of the start time of sample measurement before the start of the internal standard solution measurement sequence, or in the case of an idle cycle without sample measurement, the internal standard solution measurement is scheduled by the analysis unit of the overall control unit 134 using the control unit 134b within a time corresponding to the start time.

[0150] like Figure 12 As shown, firstly, after the internal standard solution measurement scheduling is initiated (F101), the analysis unit control unit 134b determines whether to perform sample potential measurement before the planned internal standard solution measurement (F102). At this time, if it is not a sample potential measurement, the internal standard solution measurement A is scheduled (F103).

[0151] However, the internal standard solution measurement in F103 is not problematic even when using internal standard solution measurement B. In this case, an adjustment effect can be obtained to mitigate the temperature influence during ISE measurement caused by the measurement interval being separated due to the cleaning action of internal standard solution measurement B. Therefore, when prioritizing the stabilization of the internal standard solution potential, it is possible to select internal standard solution measurement B.

[0152] If the sample potential is measured before the internal standard solution determination, then it is determined whether the sample determination is likely a high-concentration sample (F104). If it is unlikely to be a high-concentration sample, for example, if it is determined to be a sample other than a urine sample, then internal standard solution determination A is planned (F105).

[0153] In contrast, in cases where the sample may be from a high-concentration region, internal standard solution determination B (F106) is planned. In this case, internal standard solution determination C, internal standard solution determination D, or internal standard solution determination E (described later) can be planned instead of internal standard solution determination B.

[0154] The determination of whether a sample to be measured is likely to be from a high-concentration region is performed according to settings entered by the user.

[0155] For example, such as Figure 13As shown, in the setting screen 701 displayed on the display unit 131, the type of the target sample is entered in the sample type selection area 711 for ISE measurement. The user sets the concentration of the target sample in the concentration selection area 712 to be either standard or the possibility of a high concentration area sample by operating the input unit 132.

[0156] If the user selects the concentration region according to the type of sample, the type of measurement time sequence used in the measurement time sequence display area 713 will be displayed.

[0157] exist Figure 13 The diagram illustrates a scenario where a urine sample is used as a high-concentration region sample, and the internal standard solution is used to determine B in the potential measurement of the internal standard solution after the sample is measured.

[0158] Additionally, in the settings screen 701, the type of electrolyte for the object of analysis can be selected in the electrolyte category selection area 702.

[0159] It should be noted that the settings for the high-concentration area samples and the corresponding internal standard solutions are not limited to just one option, but include multiple options.

[0160] For example, as an internal standard solution determination E (second timeline), an action is prepared to reduce the amount of reagent used in the cleaning action, and as a corresponding sample setting, a setting for a medium concentration region sample can be performed.

[0161] In samples with medium concentrations, the risk of carrier infection is lower compared to those with high concentrations. Therefore, as... Figure 14 As shown, when a sample that may be of medium concentration (injection concentration) is selected in the concentration selection area 732 of the setting screen 721, the internal standard solution measurement E is set by reducing the total amount of diluent used for cleaning and internal standard solution. The "Internal Standard Solution Measurement E" is displayed as the type of measurement sequence used in the measurement sequence display area 733.

[0162] It should be noted that the criteria for determining whether a sample may be of high concentration are not limited to the information set for each sample category as described above. For example, in the case of re-inspection of a sample, the results of a previous inspection can be used.

[0163] More specifically, for example, even if the information set based on the sample type indicates that the sample may be of high concentration, but a previous inspection revealed that it was not of high concentration, the internal standard solution can still be used to determine the determination sequence of sample A during the re-inspection of that sample. Similarly, even if the information set based on the sample type indicates that the sample could not be of high concentration, but a previous inspection revealed that it was of high concentration, the internal standard solution can still be used to determine the determination sequence of samples B, C, D, and E during the re-inspection of that sample.

[0164] In addition, the reagent introduction process into the electrode flow path during the IS-1 cleaning operation may not necessarily be the same as that during the internal standard liquid potential measurement.

[0165] Generally, for cleaning, the faster the fluid flows within the flow path, the better the cleaning effect. Therefore, during the cleaning action equivalent to the idle time of the internal standard solution measurement A in the internal standard solution measurements B, C, D, and E, it is preferable to perform a syringe suction action. This syringe suction action makes the suction action of the internal standard solution and diluent to the electrode flow path during the internal standard solution potential measurement faster than any action performed during the internal standard solution potential measurement.

[0166] In addition, the reagent discharged into the dilution tank 1 can also be drawn in two batches during the determination of the internal standard solution D.

[0167] Next, the effects of this embodiment will be explained.

[0168] The automatic analysis apparatus 100 of this embodiment described above includes an electrolyte measurement unit 114 that performs an internal standard solution measurement at least once before the potential measurement of the sample. When the potential measurement of the sample performed by the electrolyte measurement unit 114 is performed continuously, the measurement operation of the internal standard solution before the potential measurement is changed according to whether the sample measured in the previous measurement may be a high-concentration sample.

[0169] In this way, idle time is created during the determination of internal standard solutions necessary for electrolyte determination, and time is added to the analytical performance stabilization sequence, which originally required the preparation of other action sequences, to achieve the same effect as the cleaning process. This is performed even if the previous sample may be a high-concentration sample. As a result, compared with the prior art, the carryover situation in the electrolyte determination section 114, represented by the dilution tank, can be reduced.

[0170] In particular, the actual electrode flow path in the electrolyte measurement section has seams between electrodes and an ion-selective membrane, creating a structure with more irregularities and a greater tendency for dirt to accumulate compared to a typical flow path. Therefore, carryover can occur not only from the dilution tank but also from residual sample solution within the electrode flow path.

[0171] However, with the control action of the present invention, even if there are uneven areas in the dilution tank 1 or the electrode flow path where dirt can easily accumulate, or if some nozzle position shifts occur, a significant reduction in carryover rate can be expected. Therefore, for the carryover performance during long-term device use, it can be a device with higher robustness than the prior art.

[0172] In addition, the potential measurement of the internal standard solution, including cleaning, can be performed in the same cycle as continuous measurement, eliminating the cycle occupied by cleaning, thus reducing the risk of reduced processing capacity in electrolyte measurement.

[0173] Furthermore, since the action change only applies to the interval IS-1 of the internal standard solution A, there is no need to prepare complex timing in the software configuration, which can be expected to simplify the design and processing of system management.

[0174] Based on the above, an automated analysis device can be provided that contributes to the stability of electrolyte data, the speed of inspection, and the stability of the data.

[0175] Since the sample concentration before measurement is unknown, it is impossible to determine whether carryover prevention measures should be implemented on the electrode flow path until the internal standard solution measurement is performed after the sample potential measurement. In contrast, using information set for each sample category as a criterion for determining potentially high-concentration samples, and considering the general concentration range of known ISE for each sample category, appropriate internal standard solution measurement actions can be set for each sample category. This allows for automatic internal standard solution measurement, including a cleaning action, in situations where the risk is high in high-concentration areas. Therefore, the risk of carryover can be significantly reduced.

[0176] Furthermore, in the case of re-inspection of samples, the risk of carryover can be reliably reduced by using the results of previous inspections, which serve as a benchmark for determining whether a sample may be of high concentration.

[0177] In addition, if the sample measured previously may be a high-concentration sample, by attracting at least one of the internal standard solution and the diluent for diluting the sample to the electrode of the electrolyte measuring section, the interior of the ion-selective electrode 7, 8, 9 and the reference electrode 10 can be cleaned, and the carryover can be reduced more effectively.

[0178] Moreover, by dividing the action of drawing at least one of the internal standard solution and diluent to the electrode into two steps, a reduction in carryover can be reliably achieved.

[0179] Furthermore, the device includes internal standard solution measurement A, which specifies the operation of the electrolyte measurement unit 114 when the previous measurement could not have been a high-concentration sample, and internal standard solution measurements B, C, D, and E, which specify the operation of the electrolyte measurement unit 114 when the previous measurement could have been a high-concentration sample. In particular, internal standard solution measurement A has an idle time at the beginning of the timing diagram during which the electrolyte measurement unit 114 is not in operation. Internal standard solution measurements B, C, D, and E are specified to perform an operation of discharging or aspirating at least one of the internal standard solution and the diluent of the diluted sample at a time corresponding to the idle time of internal standard solution measurement A. This allows for rapid switching between continuous and discontinuous operation of electrolyte measurement, enabling stable operation of the device.

[0180] Furthermore, the internal standard solution determination B is specified to perform only the action of draining and aspirating the diluent, thereby suppressing excessive increase in reagent consumption and reliably improving the accuracy of electrolyte determination.

[0181] Furthermore, the internal standard solution determination C is specified to involve the discharge and aspiration of both the internal standard solution and the diluent. Thus, even if the internal standard solution determination C is selected irregularly, the situation of using only one side of the reagent in an extreme manner can be avoided. Therefore, the deviation in the consumption of internal standard solution and diluent can be reduced, and the deviation in the frequency of reagent container replacement can be alleviated.

[0182] Furthermore, by making the aspiration action of the internal standard solution and diluent, which are attracted during the idle time of the internal standard solution measurement of B, C, D, and E, comparable to that of the internal standard solution measurement of A, faster than any action performed during the potential measurement of the internal standard solution, the cleaning effect can be improved, and the probability of carryover can be further reduced.

[0183] Furthermore, by setting the benchmark for whether a sample is likely to be a high-concentration sample in multiple stages, it is possible to suppress over-cleaning or perform actions with higher cleaning effectiveness, thereby further reducing the risk of carryover.

[0184] In addition, users can set the discharge volume of internal standard solutions and diluents in B, C, D, and E, and can also adjust the balance of cleaning treatment and reagent consumption by taking into account changes in the measurement environment, measurement interval, and other measurement conditions, as well as the device setting environment.

[0185] <Other>

[0186] Furthermore, the present invention is not limited to the embodiments described above, and various modifications and applications are possible. The embodiments described above are provided for the purpose of readily understanding and illustrating the present invention, and are not intended to limit the invention to having all the described components.

[0187] Symbol Explanation

[0188] 1…Dilution tank,

[0189] 2…sample dispensing nozzle,

[0190] 3…Diluent dispensing nozzle,

[0191] 4…Internal standard solution dispensing nozzle,

[0192] 5…sample liquid pipette tip,

[0193] 6…Piping,

[0194] 7…Sodium ion selective electrode,

[0195] 8… Potassium ion selective electrode,

[0196] 9…Chloride ion selective electrode,

[0197] 10…reference electrode,

[0198] 11...Piping,

[0199] 12…sampling pump,

[0200] 13…potential measurement section,

[0201] 14… diluent container,

[0202] 15…Internal standard liquid container,

[0203] 16…Temperature adjustment unit,

[0204] 17…Reference electrode liquid container,

[0205] 18… Pumps for diluents (DIL pumps)

[0206] 19… Internal standard solution pump (IS pump),

[0207] 100…Automatic analysis device,

[0208] 101…Transportation Unit

[0209] 102… Support for supplying trays,

[0210] 103… Bracket buffer,

[0211] 104…sample holder,

[0212] 106… conveyor line,

[0213] 107…Standard storage tray,

[0214] 111…Analysis Unit,

[0215] 112… Sample dispensing line,

[0216] 114…Electrolyte Measurement Section,

[0217] 115…reaction plate,

[0218] 116…Reagent dispensing nozzle,

[0219] 117…Reagent tray,

[0220] 118…Colorimetric Measurement Section,

[0221] 130…operation unit,

[0222] 131… Display Department,

[0223] 132… Input Section,

[0224] 133…Records Department,

[0225] 134…Overall Control Department,

[0226] 134a…Control unit for conveying unit,

[0227] 134b…Analysis unit control section,

[0228] 134c…operation and recording unit,

[0229] 601…Setup screen,

[0230] 602… Discharge volume selection area,

[0231] 603… Discharge selection area,

[0232] 701…Setup screen,

[0233] 702…Electrolyte category selection area,

[0234] 711… Sample category selection area,

[0235] 712…concentration selection area,

[0236] 713… Measurement timing display area,

[0237] 721…Setup screen,

[0238] 732…concentration selection area,

[0239] 733… Measurement timing display area.

Claims

1. An automatic analysis device, characterized in that, It is an automated analytical device for automatically analyzing samples, equipped with an electrolyte measurement unit, which performs at least one measurement of an internal standard solution before measuring the potential of the sample. When continuously performing potential measurements on the sample by the electrolyte measurement unit, the measurement operation of the internal standard solution before the potential measurement is changed based on whether the sample measured previously might be a high-concentration sample. The system includes a first timing diagram showing the operation of the electrolyte measuring unit when the previous measurement could not have been of the high-concentration sample, and a second timing diagram showing the operation of the electrolyte measuring unit when the previous measurement could have been of the high-concentration sample. The first timing diagram includes an idle time at the beginning where the electrolyte measuring unit is not operating. The second timing diagram is defined as performing an action of draining or aspirating at least one of the internal standard solution and the diluent for diluting the sample at a time corresponding to the idle time of the first timing diagram.

2. The automatic analysis device according to claim 1, characterized in that, Information set for each sample category is used as a criterion for determining whether a sample is likely to be of the high concentration type.

3. The automatic analysis device according to claim 1, characterized in that, In the case of re-inspection of the sample, the results of the previous inspection are used as a benchmark for determining whether it is a sample with high concentration.

4. The automatic analysis device according to claim 1, characterized in that, If the sample measured previously may have been a high-concentration sample, at least one of the internal standard solution and the diluent for diluting the sample is drawn to the electrode of the electrolyte measuring unit.

5. The automatic analysis device according to claim 1, characterized in that, The second timing diagram is defined as performing the action of only discharging and aspirating the diluent.

6. The automatic analysis device according to claim 1, characterized in that, The second timing diagram is defined as performing the action of draining and aspirating both the internal standard solution and the diluent.

7. The automatic analysis device according to claim 6, characterized in that, Users can set the discharge rates of the internal standard solution and the diluent in the second timing diagram.

8. The automatic analysis device according to claim 1, characterized in that, In the second timing diagram, the aspiration action of the internal standard solution and the diluent, which are aspirated at a time corresponding to the idle time in the first timing diagram, is set to be faster than any action performed during the potential measurement of the internal standard solution.

9. The automatic analysis device according to claim 1, characterized in that, The criterion for determining whether the sample is likely a high-concentration sample is set at multiple stages.

10. The automatic analysis device according to claim 4, characterized in that, The action of drawing at least one of the internal standard solution and the diluent to the electrode is divided into two steps.

11. An automated analysis method for a sample, characterized in that, This is an automated analysis method for samples performed by an automated analysis device, which includes an electrolyte measurement unit. The electrolyte measurement unit performs at least one measurement of an internal standard solution before measuring the potential of the sample. When continuously performing potential measurements on the sample by the electrolyte measurement unit, the measurement procedure of the internal standard solution before the potential measurement is changed based on whether the sample measured previously was likely a high-concentration sample. The system includes a first timing diagram showing the operation of the electrolyte measuring unit when the previous measurement could not have been of the high-concentration sample, and a second timing diagram showing the operation of the electrolyte measuring unit when the previous measurement could have been of the high-concentration sample. The first timing diagram includes an idle time at the beginning where the electrolyte measuring unit is not operating. The second timing diagram is defined as performing an action of draining or aspirating at least one of the internal standard solution and the diluent for diluting the sample at a time corresponding to the idle time of the first timing diagram.

Images