A protein analyzer

Abstract

The invention relates to a protein analyzer, comprising a base, a test tube rack transport device, a sampling mechanical arm, a reaction cup plate mechanism arranged at a corresponding position of the other end of the sampling mechanical arm, a reaction cup upper cup mechanism arranged at one side of the reaction cup plate mechanism, a reaction cup upper cup mechanical arm arranged between the reaction cup upper cup mechanism and the reaction cup plate mechanism, and a reagent loading mechanism arranged at one side of the reaction cup plate mechanism; the invention utilizes the test tube rack transport device, the sampling mechanical arm, the reaction cup plate mechanism, the reaction cup upper cup mechanism, the reaction cup upper cup mechanical arm and the reagent loading mechanism to achieve whole blood test tube on-machine detection, and when blood needs to be separated, the blood separation mechanism can be used to separate plasma, thereby achieving whole blood sampling, separation, and plasma detection processes, which are fully automatic and free of manual operation by personnel, avoid personnel contact with blood, improve the safety of personnel detection process, and reduce the possibility of sample contamination.

Description

Technical Field

[0001] The invention belongs to the technical field of detection equipment, and particularly relates to a protein analyzer. Background Art

[0002] With the rapid development of the IVD industry, global IVD technology has entered a new development model with integration, automation, intelligence, speed, miniaturization, precision, Internet big data and artificial intelligence as the core.

[0003] Some viruses can be transmitted through aerosols. For the safety of test operators, it is necessary to avoid exposing samples to the air during the test to prevent aerosol transmission. The demand for original tube machines is becoming more and more urgent across the country.

[0004] The patent application with publication number CN210572335U discloses a highly sensitive specific protein analyzer, including a shell and a power supply. The shell is provided with a detection mechanism, and the detection mechanism is provided with a detection cell. The detection mechanism is an optical detection mechanism, including a light source arranged at the front side of the detection cell to provide incident light, and a shade, a condenser and a detector fixedly arranged in sequence backward along the detection cell; the specific protein analyzer also includes a touch screen and a control chip. The touch screen is fixedly installed on the front side of the shell for inputting instructions and displaying detection results. The control chip is electrically connected to the detector and the touch screen for controlling the various electronic components of the specific protein analyzer.

[0005] For another example, the patent application with publication number CN110632288A discloses a specific protein analyzer, including: a main unit and an automatic sampler, the automatic sampler including a mixing component, an automatic sampler component and a refrigeration bin component, the automatic sampler component including a test tube rack sampler area, a test tube rack feed area and a test tube rack unloading area, the automatic sampler component is used to transport the test tube rack placed in the test tube rack sampler area to the test tube rack feed area and the test tube rack unloading area in sequence, the test tube rack includes a test tube carrying a sample to be tested, the mixing component is used to mix the test tube, and the refrigeration bin component is provided with two antibody bottle placement positions.

[0006] The above two existing patent applications have low automation levels, and under current environmental requirements, they do not take the safety of inspection operators into consideration. Summary of the invention

[0007] The technical problem to be solved by the present invention is to provide a protein analyzer with a high degree of automation, which can realize on-machine detection of whole blood test tubes.

[0008] The technical solution adopted by the present invention is:

[0009] A protein analyzer comprises a base, a test tube rack conveying device arranged on the base, a sampling mechanical arm arranged on one side of the test tube rack conveying device and used for sucking samples in a test tube, a reaction cup plate mechanism arranged at a corresponding position at the other end of the sampling mechanical arm, a reaction cup upper cup mechanism arranged on one side of the reaction cup plate mechanism, a reaction cup upper cup mechanical arm arranged between the reaction cup upper cup mechanism and the reaction cup plate mechanism, and a reagent loading mechanism arranged on one side of the reaction cup plate mechanism.

[0010] Furthermore, the reaction cup and plate mechanism includes a base, a constant temperature ring arranged on the base, a reaction plate located inside the constant temperature ring and rotatably arranged on the base, a reaction cup placed on the reaction plate, a mixing device arranged below the reaction plate and corresponding to the position of the reaction cup, and an optical path detection mechanism arranged on one side of the reaction plate;

[0011] A plurality of placement grooves are arranged at the edge of the reaction disk, and all the placement grooves form a ring shape and are concentric with the rotation center of the reaction disk.

[0012] Further, the reagent loading mechanism is arranged at an upper position on one side of the reaction cup plate mechanism, and includes a device housing, a reagent kit turntable arranged in the device housing, reagent kits arranged in a circular array on the reagent kit turntable, a reset spring arranged between the reagent kit turntable and the reagent kit, and a pressing device arranged on the device housing for pressing down the reagent kit; an air inlet connector and a liquid outlet electromagnetic switch are respectively arranged at the bottom of the device housing, and an air inlet pipeline and a nozzle are correspondingly arranged at the bottom of the reagent kit, and when the reagent kit is pressed down, the air inlet connector is connected to the air inlet pipeline, and the nozzle is connected to the liquid outlet electromagnetic switch;

[0013] A rubber plug is provided in the air intake pipe, a sealing gasket is provided above the rubber plug, a sealing spring is provided between the sealing gasket and the top of the air intake pipe, and a through hole matched with the air intake joint is provided on the rubber plug. After the test kit is pressed down, the air intake joint lifts up the sealing gasket.

[0014] Furthermore, the reaction cup loading mechanism comprises a fixing plate, a reaction cup loading plate arranged on the fixing plate, a guide rail installed on the fixing plate and located on one side of the bottom of the reaction cup loading plate, a tearing tape fixing shaft arranged above the guide rail, and a tape reel arranged above the tearing tape fixing shaft, a plurality of turns of reaction cup tape are wound in the reaction cup loading plate, the other end of the reaction cup tape is connected to the tape reel after passing the tearing tape fixing shaft from below, and a reaction cup is installed on the reaction cup tape;

[0015] The two fins on the top of the reaction cup are provided with clamping protrusions, and the reaction cup belt is provided with corresponding clamping holes.

[0016] Furthermore, a reaction cup groove is provided on the guide rail, and the cup body of the reaction cup can enter the reaction cup groove. Reaction cup baffles are provided on the left and right sides above the reaction cup groove, and a rectangular groove is formed between the reaction cup baffle and the guide rail. The extension direction of the rectangular groove is parallel to the moving direction of the reaction cup, and the two fins on the top of the reaction cup are located in the corresponding rectangular groove.

[0017] Furthermore, a rotating shaft is provided on the fixed plate, a cup plate reel is provided in the cup plate on the reaction cup, and one end of the reaction cup belt is connected to the cup plate reel;

[0018] Three L-shaped metal springs arranged in a triangle are provided inside the cup and tray reel. The cross-section of the rotating shaft is approximately triangular. An oblong hole is provided at one end of the metal spring. The metal spring is connected to the corresponding edge of the rotating shaft by arranging bolts in the oblong hole. The other end of the metal spring is in contact with the cup and tray reel.

[0019] Furthermore, the sampling mechanical arm includes a moving arm, a mounting seat arranged on the moving arm, a sampling needle arranged on the mounting seat, a needle washing assembly arranged at the bottom of the mounting seat, and a needle washing pipeline connected to the needle washing assembly; a sampling pipeline is connected to the top of the sampling needle, and a plunger pump is provided on the sampling pipeline;

[0020] The needle washing assembly includes a needle washing seat, a cleaning cavity arranged inside the needle washing seat, and a water inlet hole and a water suction hole connected to the cleaning cavity. Sealing holes are respectively arranged on the needle washing seat and located at the upper and lower ends of the cleaning cavity. The sampling needle passes through the sealing hole at the upper end to enter the cleaning cavity. The needle washing pipeline is connected to the water inlet hole and the water suction hole.

[0021] An up-and-down moving arm is arranged on the mounting seat, and the upper end of the sampling needle is fixed on the up-and-down moving arm and moves up and down with the up-and-down moving arm.

[0022] Furthermore, the needle washing pipeline includes a water suction pipeline connected to the water suction hole and a water inlet pipeline connected to the water inlet hole;

[0023] A liquid suction pump is provided on the water suction pipeline, and a waste water bucket is provided at the end of the water suction pipeline;

[0024] Two paths are connected in parallel on the water inlet pipeline, one of which is connected to the cleaning liquid bucket and is provided with a first peristaltic pump, and the other is connected to the pure water bucket and is provided with a second peristaltic pump;

[0025] An electromagnetic switching valve is arranged on the sampling pipeline, and one end of the water inlet pipeline is communicated with the electromagnetic switching valve.

[0026] Furthermore, a blood separation mechanism is provided between the test tube rack transport device and the reaction cup tray mechanism, and the blood separation mechanism includes a centrifugal disk, a transfer tray, and a centrifugal drive device arranged on one side of the transfer tray; an electromagnet is provided above the transfer tray, and a slideway is provided below the transfer tray and at a position corresponding to the electromagnet;

[0027] The centrifugal drive device comprises a centrifugal drive motor and a centrifugal chuck arranged on the output shaft of the centrifugal drive motor, a through hole for the centrifugal chuck to pass through is arranged on the transfer tray, the centrifugal plate cooperates with the centrifugal chuck, and a lifting device for moving the centrifugal drive device up and down is also arranged on the centrifugal drive device;

[0028] A accommodating barrel for placing the centrifugal disc is provided on the other side of the transfer tray, a slot hole is opened at the bottom of the accommodating barrel and on one side corresponding to the transfer tray, and a push plate corresponding to the position of the slot hole is provided on the other side of the accommodating barrel; the push plate is slidably arranged on the third guide rod through a third slider, and the third slider is fixedly connected to the third synchronous belt driven by the third displacement drive motor; the height of the slot hole is higher than the thickness of one centrifugal disc and lower than the thickness of two centrifugal discs.

[0029] Furthermore, a first gear is sleeved on the output shaft of the centrifugal drive motor, a second gear is provided on one side of the centrifugal drive motor, the second gear is arranged on the output shaft of the stepper motor, the stepper motor is connected to the displacement drive device, and the displacement drive device drives the second gear to move so that it meshes with or disengages from the first gear;

[0030] The displacement drive device includes a first synchronous belt, a first displacement drive motor for driving the first synchronous belt to move, the stepper motor is fixedly mounted on the first slider and the first slider is slidably arranged on the first guide rod, and the first slider is fixedly connected to the first synchronous belt. The positive effects of the present invention are as follows: the present invention utilizes the cooperation of the test tube rack transport device, the sampling mechanical arm, the reaction cup plate mechanism, the reaction cup upper cup mechanism, the reaction cup upper cup mechanical arm and the reagent loading mechanism to realize the whole blood test tube on-machine detection, and when the blood needs to be separated, the blood separation mechanism can be used to separate the plasma, thus realizing the whole blood sampling, separation and plasma detection process, which is fully automatic without manual operation by personnel, avoiding personnel contact with blood, improving the safety of the personnel detection process and reducing the possibility of sample contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic diagram of the structure of the present invention;

[0032] Figure 2 It is a top view schematic diagram of the present invention;

[0033] Figure 3 This is a schematic diagram of the structure of the reaction cup and tray mechanism of the present invention;

[0034] Figure 4 For the present invention Figure 3 Schematic cross-sectional view;

[0035] Figure 5 This is a schematic diagram of the structure of the cup loading mechanism of the reaction cup of the present invention;

[0036] Figure 6 This is a schematic diagram of the installation position of the driven pulley of the present invention;

[0037] FIG7 is a schematic diagram of the guide rail structure of the present invention;

[0038] Figure 8 This is a schematic diagram of the cup and plate scroll structure of the present invention;

[0039] Fig. 9 This is a schematic diagram of the reaction cup structure of the present invention;

[0040] Fig.10 This is a schematic diagram of the mounting base structure of the sampling mechanical arm of the present invention;

[0041] Fig.11 It is a cross-sectional schematic diagram of the needle washing assembly of the present invention;

[0042] Fig.12 This is a schematic diagram of the needle washing pipeline of the present invention;

[0043] Fig.13 This is a schematic diagram of the structure of the blood separation mechanism of the present invention;

[0044] Fig.14 This is a schematic diagram of the structure of the container of the present invention;

[0045] Fig.15 This is a schematic diagram of the transfer tray structure of the present invention;

[0046] Fig.16 It is a schematic diagram of the position of the electromagnet of the present invention;

[0047] Fig.17 It is a structural schematic diagram of the centrifugal drive device of the present invention;

[0048] Fig.18 This is a schematic diagram of the structure of the test tube rack transport device of the present invention;

[0049] Fig.19 This is a schematic diagram of the structure of the test tube rack propulsion mechanism of the present invention;

[0050] Fig. 20 This is a schematic diagram of the structure of the test tube rack transverse movement mechanism of the present invention;

[0051] Fig.21 This is a schematic diagram of the structure of the test tube rack ejection mechanism of the present invention;

[0052] Fig. 22It is a schematic diagram of the structure of the transport system of the present invention;

[0053] Fig.23 It is a schematic diagram of the structure of the mixing mechanism of the present invention;

[0054] Fig.24 It is a schematic diagram of the structure of the mixing unit of the present invention;

[0055] Fig.25 It is a schematic diagram of the structure of the front and rear motion unit of the present invention;

[0056] Fig.26 It is a schematic diagram of the structure of the vertical motion unit of the present invention;

[0057] Fig. 27 This is a schematic diagram of the test tube clamping state of the present invention;

[0058] Fig.28 This is a schematic diagram of the structure of the reagent loading mechanism of the present invention;

[0059] Fig.29 It is a cross-sectional schematic diagram of the reagent loading mechanism of the present invention;

[0060] Fig.30 For the present invention Fig.29 Enlarged view of point A in the middle;

[0061] Fig.31 It is a schematic diagram of the arrangement of the kit of the present invention;

[0062] Fig.32 This is a schematic diagram of the air intake pipeline structure of the present invention;

[0063] Fig.33 This is a schematic diagram of the sealing gasket structure of the present invention;

[0064] Fig.34 This is a schematic diagram of the structure of the cup loading mechanical arm of the reaction cup of the present invention;

[0065] Fig.35 This is a schematic diagram of the pick-up expansion sleeve structure of the present invention;

[0066] Fig.36 It is a schematic diagram of the structure of the blanking fork of the present invention. DETAILED DESCRIPTION

[0067] Example 1

[0068] As attached Figure 1 , 2As shown, this embodiment provides a protein analyzer, including a base 1, a test tube rack transport device 2 arranged on the base 1, a sampling mechanical arm 5 arranged on one side of the test tube rack transport device 2, a reaction disk mechanism 6 corresponding to the sampling mechanical arm 5, a reaction cup loading mechanism 3 arranged on one side of the reaction disk mechanism 6, a reaction cup loading mechanical arm 4 arranged between the reaction cup loading mechanism 3 and the reaction cup disk mechanism 6, and a reagent loading mechanism 7 arranged on one side of the reaction cup disk mechanism 6. The base 1 has two layers, the aforementioned mechanisms are located at the upper layer, and the lower layer is a waste collection box for collecting used reaction cups.

[0069] The sampling robot 5 is arranged on the slide rail and can slide along the slide rail. One end of its moving position corresponds to the position of the test tube rack transport device 2, and the other end corresponds to the position of the reaction disk mechanism 6. The test tube rack transport device 2 can transport the test tube rack 10 to the right place. The sampling robot 5 collects the sample in the test tube and then transports and adds it to the reaction cup of the reaction disk mechanism 6. The reagent loading mechanism 7 then adds the reagent to the reaction cup, and the two will react to achieve detection. The reaction cup loading mechanism 3 can realize the function of automatically loading the reaction cup. The reaction cup loading robot 4 is used to move the reaction cup from the reaction cup loading mechanism 3 to the reaction disk mechanism 6 to realize the function of adding the reaction cup to the reaction disk mechanism 6.

[0070] The cup loading robot arm 4 of the reaction cup only needs to realize simple grabbing and transferring functions, which can be realized in many solutions in the prior art and will not be described in detail here.

[0071] Taking into account the situation that some whole blood samples need to be centrifuged to separate the plasma before they can be tested, a blood separation mechanism 8 is provided between the test tube rack transport device 2 and the reaction cup plate mechanism 6, which can perform a centrifugal operation on the blood. The blood sample is first added to the blood separation mechanism 8 for centrifugation, and the centrifuged plasma is then added to the reaction plate mechanism 6 through the sampling robot arm 5.

[0072] Example 2

[0073] This embodiment further defines the test tube rack transport device 2 of embodiment 1. Figure 18-22 As shown, the test tube rack transport device 2 includes a mounting plate 202 disposed on the base 1, and a test tube rack propulsion mechanism, a test tube rack transverse movement mechanism, and a test tube rack push mechanism disposed on the mounting plate 202. The test tube rack 10 is sequentially transported along a U-shaped direction on the mounting plate 202 through the action of the test tube rack propulsion mechanism, the test tube rack transverse movement mechanism, and the test tube rack push mechanism. One end of the sampling mechanical arm 5 is located on one side of the mounting plate 202, and the position at the bottom of the U-shape can transport the test tube rack to a position closer to the detection instrument.

[0074] A slot is provided on the mounting plate 202, and the test tube rack propulsion mechanism, the test tube rack transverse movement mechanism and the test tube rack ejection mechanism are installed on the lower side of the test tube rack 10, and after passing through the slot, they are in contact with the test tube rack 10. On the mounting plate 202, along the transport direction of the test tube rack 10, a slide groove is provided correspondingly, and the test tube rack 10 slides in the slide groove, which can be used to stabilize the direction.

[0075] A conveyor belt 201 is provided on one side of the mounting plate 202 for transporting the test tube rack. A pushing device 218 is provided on the other side of the conveyor belt 201 and at a position corresponding to the test tube rack propulsion mechanism. The function of the pushing device 218 is relatively simple. As long as the test tube rack 10 can be pushed onto the mounting plate 202, a pneumatic cylinder, an oil cylinder, etc. can be used. There are many technical solutions in the prior art that can be implemented, which will not be repeated here.

[0076] Preferably, a detector 217 and an identifier are provided on one side of the conveyor belt 201 and in front of the pushing device 218.

[0077] When in use, the test tube rack 10 is moved from right to left ( Fig.18 The test tube rack is transported in the direction shown in the figure. It first passes through an identifier to identify whether the test tube rack needs to enter the testing instrument. If it needs to enter the testing instrument, after the test tube rack passes the detector 217, the push device 218 will push the test tube rack into the test tube rack entry area of ​​the test tube rack loading mechanism. The test tube rack will be pushed to the test tube sampling area by the test tube rack pushing mechanism, and the instrument will perform sampling and testing on the test tube. The test tube rack lateral movement mechanism is used to move the test tube rack horizontally out of the test tube sampling area. After entering the test tube delivery area, it is pushed to the conveyor belt 201 again by the test tube rack pushing mechanism, completing the cycle of the test tube rack 10 inside a single instrument.

[0078] The test tube rack propulsion mechanism includes a propulsion mounting plate 204 fixed to the bottom surface of the mounting plate 202, a propulsion optical axis 207 fixedly arranged on the propulsion mounting plate 204, a test tube rack propulsion frame 205 slidably mounted on the propulsion optical axis 207, a propulsion motor 208 fixedly installed on the propulsion mounting plate 204, and a propulsion synchronous belt arranged on the output shaft of the propulsion motor 208. The test tube rack propulsion frame 205 is fixedly connected to the propulsion synchronous belt, and the reciprocating motion of the propulsion synchronous belt is used to drive the test tube rack propulsion frame 205 to achieve reciprocating motion.

[0079] Preferably, matching protrusions 206 are provided at both ends of the top of the test tube rack pushing frame 205 , and the matching protrusions 206 can match with the bottom of the side wall of the test tube rack 10 after passing through the slots on the mounting plate upwards, so as to realize the pushing of the test tube rack 10 .

[0080] The transverse movement mechanism of the test tube rack includes a transverse movement mounting plate 213 fixed on the bottom surface of the mounting plate 202, a transverse movement frame 214 slidably arranged on the transverse movement mounting plate 213, and a transverse movement fork 215 arranged on the transverse movement frame 214 through a rotating shaft. The transverse movement fork 215 protrudes upward from a part of the transverse movement frame 214 and can be matched with a matching groove at the bottom of the test tube rack 10.

[0081] Limiting posts 219 are provided on both sides of the transverse shift fork 215. In a natural state, the transverse shift fork 215 is in an inclined state due to its own weight. Fig. 20 The right side wall in the state shown is in contact with the limit column 219, so when the transverse frame 214 moves to the right, the transverse fork 215 can push the test tube rack 210 to move to the right, and after moving one station, the transverse frame 214 moves to the left, and the transverse fork 215 will rotate clockwise under the pressure of the test tube rack 10, and will not drive the test tube rack 10 to move. After the transverse frame 214 retreats one test tube station, it moves to the right again, and pushes the test tube rack 10 forward one station again. In this way, the test tube rack 10 can be gradually pushed to the right. At the same time, this also requires that a plurality of matching grooves are provided at the bottom of the test tube rack 10 to facilitate the forward advancement of each station.

[0082] Preferably, a transverse motor 216 is provided on the transverse mounting plate 213, a transverse synchronous belt is provided on the output shaft of the transverse motor 216, and the transverse frame 214 is fixedly connected to the transverse synchronous belt.

[0083] The test tube rack pushing mechanism includes a pushing mounting plate 209 fixedly arranged on the bottom surface of the mounting plate 202, a pushing optical axis 211 arranged on the pushing mounting plate 209, and a test tube rack pushing frame 210 slidably arranged on the pushing optical axis 211. Protruding pushing forks are provided at both ends of the top of the test tube rack pushing frame 210. The pushing forks protrude upward and pass through the slots on the mounting plate 202 to cooperate with the test tube rack 10.

[0084] Preferably, a push-out motor 212 is provided on the push-out mounting plate 209 , a push-out synchronous belt is provided on the output shaft of the push-out motor 212 , and the test tube rack push-out frame 210 is fixedly connected to the push-out synchronous belt.

[0085] The test tube rack transport device 2 utilizes the three mechanisms of the test tube rack propulsion mechanism, the test tube rack transverse movement mechanism and the test tube rack push mechanism to realize the transport of the test tube rack along the U-shaped direction, and can transport the test tube rack to a position closer to the detection machine for easy access by the machine. At the same time, a conveyor belt is equipped to transport the test tube rack. When the test tube rack is transported to the position, the push device can push the test tube rack onto the test tube rack propulsion mechanism, and the test tube rack push mechanism can push the used test tube rack onto the conveyor belt again, thereby realizing automatic transportation.

[0086] This embodiment can also realize multiple machines in parallel, and the test tube rack transport devices 2 are arranged in series. Fig. 22 As shown, several test tube rack conveying devices are arranged in sequence, and the detection instruments are arranged side by side on one side of the test tube rack conveying system. By connecting data cables, the test tube racks can be assigned to each instrument for detection through the same operating system.

[0087] The present invention realizes automatic distribution of sampling tubes among multiple instruments, reduces the operation of detection personnel, reasonably distributes the detection tasks of each instrument, and improves detection efficiency.

[0088] Example 3

[0089] This embodiment further defines the cuvette loading mechanism 3 of the embodiment 1. As shown in FIGS. 5-9 , the cuvette loading mechanism 3 of this embodiment includes a fixing plate 313 disposed on the base 1, a cuvette upper cup plate 301 disposed on the fixing plate 313, a guide rail 305 mounted on the fixing plate 313 and located at one side of the bottom of the cuvette upper cup plate 301, a tearing tape fixing shaft 304 disposed above the guide rail 305, and a take-up roll 308 disposed above the tearing tape fixing shaft 304. The lower end of the guide rail 305 is fixed on the slideway fixing plate 317. n (n≥1) turns of cuvette tape 306 are wound in the cuvette upper cup plate 301, and a cuvette 302 is mounted on the cuvette tape 306. A cup plate roll 310 is disposed at the center of the cuvette upper cup plate 301. One end of the cuvette tape 306 is wound on the cup plate roll 310, and the other end is passed under the tearing tape fixing shaft 304 and then connected to the take-up roll 308. A cuvette groove is provided on the guide rail 305 , and the body of the cuvette 302 can enter into the cuvette groove, where the cuvette belt 306 is separated from the cuvette 302 .

[0090] The reaction cup includes a cup body and fins arranged on both sides of the top of the cup body. The fins are provided with snap-fit ​​protrusions 322 , and the reaction cup belt 306 is provided with corresponding snap-fit ​​holes. In this embodiment, the snap-fit ​​protrusions 322 are spherical.

[0091] A rotating shaft 320 is disposed on the fixing plate 313 , the cup and tray reel 310 is disposed on the rotating shaft 320 , and there is damping between the cup and tray reel 310 and the rotating shaft 320 .

[0092] A driving motor 309 is installed on the fixed plate 313, and a driving pulley 323 is provided on its output shaft. The driving pulley 323 drives the driven pulley to rotate through the synchronous belt 311. The driven pulley is installed on the take-up shaft 307. The take-up reel 308 is installed coaxially with the driven pulley 312, thereby driving the take-up reel 308 to rotate.

[0093] A photoelectric switch 321 is provided at the front end of the guide rail 305, and its function is to detect in real time whether there is a reaction cup 302 at the position opposite to it. If it is determined that there is a reaction cup 302, it means that the reaction cup has reached the predetermined position, and the feedback signal causes the reaction cup to stop moving forward. If it is determined that there is no reaction cup, it means that the reaction cup has not reached the predetermined position, and the feedback signal causes the reaction cup to continue moving forward.

[0094] Reaction cup baffles 316 are provided above the guide rail 305 and on the left and right sides of the reaction cup slot. There is a gap between the reaction cup baffle 316 and the top surface of the guide rail 305. The gap can be regarded as a rectangular groove, which can play a guiding role. When the reaction cup is transported to the guide rail 305, the fin is located in the gap. The extension direction of the rectangular groove is parallel to the moving direction of the reaction cup, which is used to ensure the accuracy of the direction of the reaction cup during its forward movement.

[0095] Preferably, the distance between the two reaction cup baffles 316 should be greater than the width of the reaction cup belt 306 and less than the width of the reaction cup fin, so as to ensure that the reaction cup is always in the rectangular groove, and the reaction cup belt 306 can be detached from the reaction cup 302 at the position of the tear belt fixing shaft 304, and the reaction cup belt 306 can be torn off the reaction cup 302.

[0096] Preferably, a guide wheel 303 is provided between the guide rail 305 and the reaction cup upper cup plate 301, and the guide wheel 303 is installed on the guide shaft 318. It is used to make the top of the reaction cup 302 on the reaction cup belt 306 become horizontal before entering the reaction cup groove, so that the fin of the reaction cup can smoothly enter the rectangular groove formed by the guide rail 305 and the reaction cup baffle 316.

[0097] The take-up shaft 307 is installed on the bearing seat 314 through a bearing. The take-up roll 308 is provided with a special-shaped hole. The part connected to the take-up shaft 307 corresponds to the special-shaped shaft. After the take-up roll 308 is installed, the spring glass bead 315 clamps the take-up roll 308, so that the take-up roll 308 rotates synchronously with the take-up shaft 307.

[0098] The cross-section of the rotating shaft 320 is approximately triangular, and an L-shaped metal spring piece 319 is provided on each face thereof. An oblong hole is opened on the metal spring piece 319, and a bolt is installed in the oblong hole to connect the metal spring piece 319 to the rotating shaft 320. The outer end of the metal spring piece 319 is in contact with the cup and plate reel 310. By adjusting the installation position of the bolt in the oblong hole, the compression amount between the spring piece and the inner shaft hole changes, the elastic force changes, and the damping force changes. The clamping force between the metal spring piece 319 and the cup and plate reel 310 can be adjusted to achieve appropriate damping. In this way, the cup and plate reel 310 will not rotate at will.

[0099] In summary, the reaction cup loading mechanism 3 realizes the automatic loading of disposable reaction cups, avoids the situation of repeated use of reaction cups in the prior art, and greatly improves the working efficiency and accuracy of the detection results of the entire detection instrument.

[0100] When the embodiment is in use, the take-up roll 308 mounted on the take-up shaft 307 rotates clockwise under the driving of the driving motor 309 and the action of the synchronous belt 311, and the take-up roll 308 pulls the reaction cup belt 306, and at the same time, the cup plate 301 on the reaction cup rotates clockwise around the damped rotating shaft 320. The reaction cups 302 neatly arranged on the reaction cup belt 306 move toward the guide rail 305 and enter the guide rail 305. When the reaction cup 302 passes under the tearing belt fixing shaft 304, the reaction cup 302 will gradually disengage from the reaction cup belt 306. When the reaction cup is detected by the photoelectric switch 321, the driving motor 309 stops rotating. If a reaction cup is taken out from the front end of the guide rail 305, the photoelectric switch 321 will not detect the reaction cup 302, and the driving motor 309 starts rotating until the photoelectric switch detects that the reaction cup has been loaded, so that the reaction cup is automatically loaded.

[0101] Example 4

[0102] The present embodiment further defines the reaction cup and disk mechanism 6 in the embodiment 1. As shown in FIGS. 3 and 4 , the reaction cup and disk mechanism 6 in the present embodiment includes a base 601, a constant temperature ring 603 fixed on the base 601, a reaction disk 602 nested in the constant temperature ring 603, and a mixing device 605 and an optical path detection mechanism 606 arranged on one side of the reaction disk 602.

[0103] The constant temperature ring 603 is annular, and the reaction disk 602 is also circular. A circle of placement grooves is provided on the reaction disk 602 and near its edge, and the reaction cups 302 are provided in the placement grooves. All the placement grooves form a circle, which is concentric with the center of the reaction disk 602. The mixing device 605 and the optical path detection mechanism 606 are correspondingly arranged at the position where the reaction cup 302 passes, and the reaction cup 302 has the function of mixing and detecting.

[0104] In this embodiment, the total number of placement slots is 48.

[0105] The nested mechanism is adopted, and the reaction disk 602 is completely nested in the constant temperature ring 603 to provide the best reaction temperature for the reaction cup 302. At the same time, the reaction disk has a total of 48 reaction cup stations and multiple different operation positions. By rotating the reaction disk, the reaction cup is sent to different stations for operation, realizing simultaneous multi-station collaborative operation and improving the batch detection efficiency of the instrument.

[0106] At the same time, it is also equipped with an optical inspection station, a rotating reaction disk, and direct inspection to save inspection time.

[0107] The mixing device includes a mixing motor fixed on the base 601 and a magnet arranged on the output shaft of the mixing motor. Magnetic beads are arranged in the reaction cup 302. The rotation of the motor drives the magnet to rotate, thereby driving the movement of the magnetic beads in the reaction cup 302, thereby driving the liquid to stir and achieve mixing.

[0108] Preferably, two mixing devices are provided, which are located at different positions, so that the added sample and reagent can be mixed at the same time, saving reaction time.

[0109] A transmission shaft 609 is connected to the bottom of the reaction disk 602 by bolts, and a power motor 608 is provided on the base 601 . The transmission shaft 609 is connected to the output shaft of the power motor 608 by belt transmission to realize the rotation of the reaction disk 602 .

[0110] When this embodiment is in use, the robot arm is used to place the reaction cup 302 into the designated placement slot of the reaction disk 602, and the reaction cup is pulled out by the fork 608. The reaction cup 302 rotates with the reaction disk 602 to the reagent loading station to add reagents, and then rotates to the sample loading station to add samples. The mixing device mixes the reaction liquid synchronously, and then rotates to the reagent loading station to add reagents. At the same time, the mixing mechanism mixes synchronously, and the reaction cup 302 waits for the reaction to be completed in the constant temperature ring 603. Rotate to the optical path detection station for detection. After completion, the robot arm removes the waste liquid and removes the reaction cup. The entire reaction cup circulation process on the disk is completed.

[0111] Because there are multiple reaction cup positions and multiple reaction process operation positions on the reaction tray, multiple reaction cups can perform different process operations at the same time, which greatly improves the batch detection efficiency of batch instruments.

[0112] Example 5

[0113] The present embodiment further defines the sampling robot arm 5 in the embodiment 1. As shown in FIGS. 10-12 , the sampling robot arm 5 in the present embodiment includes a mounting seat 501, an up-and-down movable arm 521 disposed on the mounting seat 501, a sampling needle 502 with one end fixed on the up-and-down movable arm 521, a sampling pipeline 515 connected to the top of the sampling needle 502, and a needle washing assembly 511 disposed at the bottom of the mounting seat 501 and corresponding to the sampling needle 502, and a needle washing pipeline is connected to the needle washing assembly 511.

[0114] Preferably, an electromagnetic switching valve 503 and a plunger pump 504 are provided on the sampling pipeline 515 .

[0115] The needle washing assembly 511 includes a needle washing seat 516, a cleaning chamber 517 arranged inside the needle washing seat 516, and a water inlet hole 518 and a water suction hole 519 arranged on the side wall of the needle washing seat 516 and connected to the cleaning chamber 517. The water inlet hole 518 is located above the water suction hole 519. Sealing holes 520 are respectively opened at the upper and lower ends of the needle washing seat 516, and the positions of the sealing holes 520 correspond to the positions of the cleaning chamber 517. The cleaning chamber 517 is a vertically arranged long groove. The sampling needle 502 can be inserted into the cleaning chamber 517 from the sealing hole 520 at the top of the needle washing seat 516, and then pass through the sealing hole 520 at the bottom.

[0116] The size of the sealing hole 520 corresponds to the size of the sampling needle 502 . When the sampling needle 502 passes through the needle washing assembly 511 , the cleaning chamber 517 is in a sealed state, ensuring that the water in the cleaning chamber 517 can be discharged from the water absorption hole 519 .

[0117] The needle washing pipeline includes a water suction pipeline 514 connected to the water suction hole 519 and a water inlet pipeline 513 connected to the water inlet hole 518. A liquid suction pump 510 and a waste water bucket 509 are provided on the water suction pipeline 514. Two pipelines are connected in parallel on the water inlet pipeline 513, one of which is a cleaning liquid delivery pipeline, on which a first peristaltic pump 508 and a cleaning liquid bucket 506 are provided, and the other pipeline is a pure water delivery pipeline, on which a second peristaltic pump 507 and a pure water bucket 505 are provided. One end of the water inlet pipeline 513 is connected to the electromagnetic switching valve 503.

[0118] The sampling and cleaning process of the sampling needle is as follows: the sampling needle 502 moves to the top of the test tube 512, moves downward to below the sample liquid level in the test tube, the electromagnetic switching valve 503 connects the sampling needle 502 and the plunger pump 504, the plunger pump 504 starts to absorb a quantitative sample, and then stops. The sampling needle 502 starts to move upward to complete the sampling. After the sampling needle 502 moves to the top, the mounting seat 501 moves to the top of the reaction cup where the sample is placed, the sampling needle 502 starts to move downward to the inside of the reaction cup, the plunger pump 504 is turned on, the sample in the sampling needle 502 is pushed into the reaction cup, and the sampling needle 502 starts to move upward. At the same time, the suction pump 510 and the first peristaltic pump 508 are turned on, the cleaning liquid is drawn from the cleaning liquid barrel 506, sent to the needle washing assembly 511, and the outer wall of the sampling needle 502 is washed, and the waste liquid flows into the waste liquid barrel 509 through the suction pump 510. After the sampling needle 502 moves to the top, the bottom of the sampling needle 502 is still in the cleaning chamber, and the electromagnetic switching valve 503 is switched to disconnect the plunger pump 504. The sampling needle 502 is connected to the second peristaltic pump 507, and the cleaning liquid rinses the inner wall of the sampling needle 502, flows out from the hole at the end of the needle, and flows into the waste liquid bucket 509 through the suction pump 510. After the cleaning is completed, the first peristaltic pump 508 is turned off, the second peristaltic pump 507 is turned on, and the cleaning liquid is changed to pure water for cleaning, and the cleaning of the inner wall of the sampling needle 502 is completed. When cleaning the inner wall, a part of the cleaning liquid and pure water will also enter the cleaning chamber through the water inlet pipe 513 to clean the outer wall, but it does not affect the normal cleaning of the inner wall.

[0119] When the sampling needle moves downward next time, the suction pump 510 and the second peristaltic pump 507 are turned on, and the outer wall of the sampling needle 502 is cleaned with pure water.

[0120] The cleaning of the outer wall and the inner wall of the sampling needle 502 of the technical solution of this embodiment is completed during the sampling and liquid transfer process of the sampling needle 502, and no separate cleaning step is required, thus saving the operation time of the equipment.

[0121] The needle washing assembly 511 is arranged at the bottom of the mounting seat 501, and the vertical moving arm 521 is arranged on the mounting seat 501 to realize the vertical movement of the sampling needle 502. The structure of the vertical moving arm 521 is not limited, as long as the vertical movement of the sampling needle 502 can be realized. There are many embodiments in the prior art, which are common knowledge for those skilled in the art and will not be repeated here.

[0122] The needle washing seat 516 includes a seat body and a cover installed on the top of the seat body by bolts. A cleaning cavity 517 is processed in the seat body, and the upper sealing hole 520 is located on the cover. The single-side gap between the two sealing holes and the sampling needle 502 is 0.01mm. The upper cover, the seat body and the sampling needle 502 form a relatively closed inner cavity. When the water absorption hole 519 absorbs water, a negative pressure environment is formed, which effectively prevents the waste liquid from flowing out from the upper and lower sealing holes passing through the sampling needle 502.

[0123] When cleaning the end and inner wall of the sampling needle, when the water absorption hole 519 absorbs water, due to the negative pressure effect in the cleaning chamber 517, a large amount of gas enters the lower sealing hole 520 under the pressure of atmospheric pressure to prevent water from flowing out of the lower sealing hole.

[0124] At the same time, the mounting seat 501 should be installed on the slide rail to achieve translation of the position. This technology is widely embodied in the prior art and will not be described in detail here.

[0125] The needle washing assembly of this embodiment has a simple structure and is easy to process. The sealing holes at both ends of the washing chamber are designed to match the outer diameter of the sampling needle, which effectively prevents the washing liquid from flowing out from the bottom, resulting in better effect.

[0126] Example 6

[0127] This embodiment further defines the blood separation mechanism 8 in Embodiment 1. As shown in FIGS. 13-17 , the blood separation mechanism 8 in this embodiment includes a transfer tray 812 for transporting the centrifugal disk 806 and a centrifugal drive device disposed on one side of the transfer tray 812 .

[0128] The centrifugal drive device includes a centrifugal drive motor 818 and a centrifugal chuck 820 mounted on its output shaft. The centrifugal drive motor 818 can move up and down under the drive of the lifting device. A through hole for the centrifugal chuck 820 to pass through is opened on the transfer tray 812, and a hole that can match the centrifugal chuck 820 is provided at the center of the centrifugal plate 806. In this embodiment, the lifting device is a first screw motor 817 arranged vertically.

[0129] Meanwhile, a first gear 819 is sleeved on the output shaft of the centrifugal drive motor 818 and below the centrifugal chuck 820, a stepper motor 823 is provided on one side of the centrifugal drive motor 818, and a second gear 822 is provided on the output shaft of the stepper motor 823. A first guide rod 827 is also provided, on which a first slider 825 is slidably provided, the first slider 825 is connected to a first synchronous belt 826, the first displacement drive motor 824 drives the first synchronous belt 826 to move, and the first slider 825 is driven to move on the first guide rod 827 through the movement of the first synchronous belt 826, and the stepper motor 823 is fixedly mounted on the first slider 825, thereby realizing the movement of the second gear 822, and realizing the meshing or disengagement of the second gear 822 with the first gear 819.

[0130] Preferably, a spring glass bead structure is provided on the centrifugal chuck 820, a special-shaped hole corresponding to the centrifugal chuck 820 is provided on the centrifugal disk 806, and a top plate 821 is provided above the centrifugal chuck 820. When the transfer tray 812 moves to the centrifugal drive device position, the transfer tray 812 is located below the top plate 821.

[0131] Blood injection holes 828 are correspondingly provided on the top plate 821 and the centrifugal disk 806 .

[0132] When the present embodiment is in use, the first screw motor 817 can lift up the centrifugal drive motor 818, the first gear 819, and the centrifugal chuck 820 when rotating, so that the centrifugal chuck 820 is inserted into the central positioning hole of the centrifugal disk 806, and the centrifugal chuck 820 continues to lift up the centrifugal disk 806 until the upper surface of the centrifugal disk 806 presses against the top plate 821, and then the centrifugal chuck 820 continues to lift up, and the centrifugal chuck 820 will be inserted into the central hole of the centrifugal disk 806. The centrifugal chuck 820 has a spring glass bead structure, and the centrifugal chuck 820 cooperates with the special-shaped hole in the center of the centrifugal disk, so that the centrifugal chuck and the centrifugal disk will be relatively fixed, and then the screw motor 817 rotates in the opposite direction, and the centrifugal disk 806 will fall. When the centrifugal disk 806 is not in contact with the top plate 821 and the transfer tray 812, the first displacement drive motor 824 drives the first synchronous belt 826 to rotate, and the first slider 825 fixedly connected to the first synchronous belt 826 will move along the first guide rod 827, that is, along the Fig.18 The stepper motor 823 connected to the first slider 825 and the second gear 822 fixed on its shaft move in the direction of the arrow until the second gear 822 is fully meshed with the first gear 819. The first displacement drive motor 824 stops rotating, and then the stepper motor 823 rotates, and the second gear 822 rotates accordingly, so that the first gear 819 meshing therewith rotates. The first gear 819 is coaxial with the centrifugal chuck 820, so the centrifugal chuck 820 rotates together, and the centrifugal chuck 820 drives the centrifugal disk 806 to rotate. When the blood addition hole on the centrifugal disk 806 is aligned with the center of the blood addition hole 828 on the top plate 821, the stepper motor 823 stops rotating, and the centrifugal disk 806 stops rotating to perform the blood addition operation.

[0133] After the blood addition action is completed, the first displacement drive motor 824 rotates in the opposite direction, causing the first synchronous belt 826 to rotate in the opposite direction of the arrow, and the second gear 822 moves away from the first gear 819. After the second gear 822 is completely separated from the first gear 819, the centrifugal drive motor 818 starts to rotate, and the centrifugal chuck 820 drives the centrifugal disk 806 to perform centrifugal action.

[0134] After the centrifugation is completed, the first displacement drive motor 824 rotates to make the second gear 822 fully mesh with the first gear 819, and then the stepper motor 823 rotates to align the blood adding hole of the centrifugal disk 806 with the center of the blood adding hole 828 on the top plate 821 to perform the serum extraction operation. After the operation is completed, the first displacement drive motor 824 rotates in the opposite direction to make the second gear 822 completely disengage from the first gear 819, and then the screw motor 817 rotates to make the centrifugal chuck 820 fall, and the centrifugal disk 806 falls. During the falling process, the centrifugal disk 806 will be supported by the transfer tray 812, and the centrifugal chuck 820 continues to fall and disengage from the centrifugal disk 806. When the centrifugal chuck 820 is completely disengaged from the positioning hole of the centrifugal disk 806 and the centrifugal chuck 820 is lower than the lower surface of the transfer tray 812, the transfer tray 812 can carry the centrifugal disk 806 for the disc withdrawal action.

[0135] Preferably, the transfer tray 812 is disposed on the second guide rod 811 via the second slider 809 , the second guide rod 811 is fixedly connected to the second synchronous belt 813 , and the second displacement drive motor 808 drives the second synchronous belt 813 to operate.

[0136] A receiving barrel 807 is provided on the other side of the transfer tray 812. Several centrifugal discs 806 are stacked in the receiving barrel 807. A rectangular groove is provided on the edge of the receiving barrel 807 in a "convex" shape to facilitate the storage and access of the circular centrifugal discs. A slot hole is provided at the bottom of the receiving barrel 807 and on one side corresponding to the transfer tray 812. The height of the slot hole is higher than the thickness of one centrifugal disc and lower than the thickness of two centrifugal discs. A push plate 804 corresponding to the position of the slot hole is provided on the other side of the receiving barrel 807. The push plate 804 can be used to push the centrifugal disc 806 at the bottom of the receiving barrel 807 out of the slot hole and onto the transfer tray 812.

[0137] The push plate 804 is slidably disposed on the third guide rod 805 via the third slider 803 , and the third slider 803 is fixedly connected to the third synchronous belt 802 driven by the third displacement driving motor 801 .

[0138] At the same time, an electromagnet 814 is provided between the accommodating barrel 807 and the centrifugal drive device and above the transfer tray 812, a slideway 815 is provided below the transfer tray 812 and at a position corresponding to the electromagnet 814, and a base plate 816 with an opening is provided at the bottom end of the slideway 815.

[0139] The transfer tray 812 carries the centrifugal disk 806 after centrifugation. Fig.16When the transfer tray 812 moves to the bottom of the electromagnet 814 in the direction of the arrow shown, the extended end of the electromagnet 814 is inserted into the central hole of the centrifugal disk 806, and the insertion depth is less than the thickness of the centrifugal disk 806. When the transfer tray 812 continues to move in the direction shown in the figure, the centrifugal disk 806 is blocked by the extended end of the electromagnet, and the centrifugal disk 806 can no longer follow the transfer tray 812 to continue to move forward. When the centrifugal disk 806 escapes from the transfer tray 812, it falls into the slideway 815 below, and then falls into the centrifugal waste disk collection box on the lower layer of the base 1. At the same time, the electromagnet 814 is turned off, and the centrifugal disk 806 continues to fall along the slideway 815 to the opening of the base plate 816, completing the disk withdrawal action.

[0140] The workflow of this embodiment is:

[0141] The third displacement drive motor 801 drives the push plate 804 to push the bottom centrifugal disk 806 in the centrifugal disk accommodating barrel 807 into the transfer tray 812 through the belt transmission, and then the second displacement drive motor 808 drives the transfer tray 812 to transport the centrifugal disk 806 to the centrifugal chuck 820 through the belt transmission, and then the centrifugal drive motor 818, the stepper motor 823, the second gear 822, the first gear 819, and the screw motor 817 cooperate to move the centrifugal disk 806 from the transfer tray 812, and then the centrifugal disk 806 is centrifugally moved. After centrifugation is completed, the transfer tray 812 carries the centrifugal disk 806 back. During the return process, when the centrifugal disk 806 reaches the position of the electromagnet 814, the protruding end of the electromagnet 814 is inserted into the centrifugal disk hole, blocking the centrifugal disk 806 but not blocking the transfer tray 812, so that the centrifugal disk 806 is disengaged from the transfer tray 812, and then the centrifugal disk 806 falls into the slide 815 and slides into the hole 16, completing the automatic disk return operation.

[0142] In this embodiment, the centrifugal disk is transferred to the centrifugal drive device by using a transfer tray, and the centrifugal chuck on the centrifugal drive device cooperates with the centrifugal disk to centrifuge the blood in the centrifugal disk to realize the centrifugal function. The whole process is completed automatically without human intervention.

[0143] It is also equipped with a accommodating barrel in which several centrifugal discs can be stacked. The centrifugal disc at the bottom can be pushed onto the transfer tray using a push plate, thus realizing the automatic centrifugal disc loading operation.

[0144] In the blood separation mechanism, centrifugal discs are pre-stored. Through the operation of the mechanism, the centrifugal discs move to the centrifugal station, and start centrifugation after adding blood. The plasma is centrifuged and extracted. The centrifugal discs will be moved to the throwing disc position and thrown out, and the centrifugal waste discs will enter the centrifugal waste disc collection box.

[0145] The whole process does not require human intervention, and only requires placing a number of centrifugal discs into the containing barrel 807, which greatly improves the automation level of the instrument.

[0146] Example 7

[0147] This embodiment is a further supplement to Embodiment 1. Figure 1 As shown, a test tube mixing mechanism 9 is provided on the base 1 and located on one side of the test tube rack 10. Figure 23-27 As shown, the test tube mixing mechanism 9 of this embodiment includes a mixing unit 9100 , a forward and backward motion unit 9200 , and a vertical motion unit 9300 .

[0148] The mixing unit 9100 includes a mixing motor 9105, a driving pulley 9107 installed on the output shaft of the mixing motor 9105, a driven pulley 9103 connected to the driving pulley 9107 through a synchronous belt 9106, a first mounting seat installed on the driven pulley 9103, and a test tube clamp arranged on the first mounting seat.

[0149] The mixing process is that the forward and backward motion unit 9200 first moves toward the test tube rack 10, the test tube clamp clamps the test tube, and then the vertical motion unit 9300 drives the forward and backward motion unit 9200 to move upward, raising the test tube so that its bottom is higher than the height of the test tube in the test tube rack 10, and then performs a rotation mixing operation, and returns to the initial position after the mixing is completed.

[0150] Preferably, the first mounting seat is coaxially mounted with the driven pulley 9103 and rotates together with the driven pulley 9103 .

[0151] The test tube clamp includes a first clamping jaw 9104 and a second clamping jaw 9109 which are rotatably arranged on a first mounting seat through a rotating shaft. The two clamping jaws are arranged opposite to each other, and a compression spring is provided between the tails of the two clamping jaws, and the heads are open. The rotating shaft is arranged in the middle position. Under normal conditions, the compression spring is not subjected to force. When the test tube clamp is used to clamp the test tube, the two clamping jaws open wider, and the tail ends are close to each other, compressing the compression spring. The reaction force of the compression spring will clamp the test tube. At the same time, the upper surface of the test tube clamp will clamp the lower edge of the test tube cap to prevent the test tube from sliding down.

[0152] When the test tube clamp clamps the test tube, the mixing motor 9105 can rotate, driving the driving pulley 9107 to rotate, and then driving the driven pulley 9103 to rotate through the synchronous belt 9106. The test tube clamp is fixedly connected to the driven pulley 9103, so that the clamped test tube rotates for mixing.

[0153] Preferably, a pressing sheet 9108 is provided on the first mounting seat and above the test tube clamp to block the top of the test tube and prevent it from being thrown out from one side of the test tube cap during mixing.

[0154] At the same time, a rotating mark piece 9102 is provided on the rotating shaft of the driven pulley 9103, and a first photoelectric switch 9101 is provided above the rotating mark piece 9102 for controlling the rotation angle of the test tube clamp. The rotating mark piece 9102 and the driven pulley 9103 rotate coaxially. When the rotating mark piece 9102 rotates to the first photoelectric switch 9101, the required rotation angle is reached, the mixing motor 9105 stops rotating, the mixing motor rotates in the opposite direction, and the test tube swings back, and so on and so forth, so that the liquid in the test tube can be mixed.

[0155] The forward and backward motion unit 9200 includes a second mounting seat and a lead screw motor 9201 and a slide rail 9206 arranged on the first mounting seat.

[0156] Preferably, the second mounting seat includes a second mounting plate 9202, a third mounting plate 9205 and a fourth mounting plate 9207 which are vertically arranged, the screw motor 9201 is arranged on the second mounting plate 9202, and the slide rail 9206 is arranged on the fourth mounting plate 9207. The mixing motor 9105 of the mixing unit 9100 is arranged on the first mounting plate 9203, a nut 9204 is installed on the first mounting plate 9203, a screw of the screw motor 9201 is connected to the nut 9204, a first slider 9209 is installed below the mixing motor 9105, the first slider 9209 is slidably connected to the slide rail 9206, and the screw motor 9201 is used to push the mixing unit 9100 to move forward and backward on the slide rail 9206.

[0157] Preferably, a second marking piece 9208 is provided on the first sliding block 9209, and a second photoelectric switch 9210 is provided on the second mounting seat.

[0158] When the mixing unit 9100 moves toward the direction approaching the screw motor 9201, the test tube clamp can clamp the test tube. If the mixing unit 9100 moves toward the direction away from the screw motor 9201, when the second marking piece 9208 moves to the second photoelectric switch 9210, the screw motor 9201 stops rotating and the test tube clamp stops moving backward.

[0159] The vertical motion unit 9300 includes a vertical mounting plate 9305, a vertical motion drive motor 9301 arranged at the bottom of the vertical mounting plate 9305, a guide rail 9303 installed on the vertical mounting plate 9305, and a second slider 9302 arranged on the guide rail 9303. The front-to-back motion unit 9200 is fixedly mounted on the second slider 9302, and the vertical motion drive motor 9301 drives the second slider 9302 to reciprocate vertically along the guide rail 9303 through a synchronous belt drive.

[0160] A third photoelectric switch 9304 is provided at the top position of the vertical mounting plate 9305, and a third marking piece 9306 is provided on the second slider 9302 or the front-rear moving unit 9200. When the third marking piece 9306 moves from bottom to top to the third photoelectric switch 9304, the vertical motion driving motor 9301 stops rotating.

[0161] This embodiment uses a test tube clamp to clamp the test tube, and then uses a mixing motor to directly drive the test tube to swing, which can achieve a higher frequency of swinging action and higher mixing efficiency. At the same time, due to direct drive, the swinging range of the test tube is smaller during the swinging process, which makes the entire shaking device occupy less space and is conducive to the rational arrangement of the entire machine parts.

[0162] At the same time, the test tube clamp can be moved in two directions under the drive of the front and rear motion units and the vertical motion units, and can automatically grab the test tubes on the test tube rack for mixing.

[0163] Example 8

[0164] This embodiment further defines the cup loading robot arm 4, as shown in the attached Figure 34-36 As shown, in this embodiment, the reaction cup loading robot 4 includes a movable base 402 that can move laterally, an upper and lower slide plates 404 arranged on the movable base 402, and a picking sleeve 405 fixedly arranged at the bottom of the upper and lower slide plates 404. The picking sleeve 405 is interference fit with the test cup, and a material discharge fork is provided below the picking sleeve 405 and at the target position for the test cup to move. The material discharge fork is used to push the test cup off the picking sleeve 405 to make the test cup fall into the target position.

[0165] As attached Figure 3 As shown, the unloading fork includes a fork base, a fork driving device 406 fixedly mounted on the fork base, and a positioning fork 407 mounted on the fork driving device 406. Fins for cooperating with the positioning fork 407 are provided on both sides of the top of the test cup.

[0166] Preferably, the fork driving device 406 is an electromagnet.

[0167] The lower end and four side edges of the pick-up expansion sleeve 405 are chamfered.

[0168] The movable base 402 is fixedly connected to the test cup transverse moving belt 401 and is driven to move transversely by the test cup moving belt 401 .

[0169] The moving base 402 is provided with a test cup vertical moving belt 403 , and the upper and lower slide plates 404 are fixedly connected to the test cup vertical moving belt 403 , and are driven to move up and down by the test cup vertical moving belt 403 .

[0170] The picking process of the present invention is as follows: the picking expansion sleeve 405 is interference-fitted with the test cup to lift and transfer the test cup; the lower end of the picking expansion sleeve is chamfered to facilitate the insertion and positioning of the expansion sleeve; the four side chamfers are equivalent to air leakage grooves, and when the expansion sleeve is inserted into the test cup, the gas in the test cup can escape.

[0171] The process of placing the test cup into the target position is as follows: after the expansion sleeve is picked up and driven to put the test cup into the work station block, the electromagnet drives the positioning fork 7 to move forward to the upper part of the test cup to block the upper edge of the test cup, and the expansion sleeve is picked up and moved upward. The test cup cannot move upward due to being blocked by the positioning fork, and falls off the expansion sleeve and remains in the target work station of the test cup.

[0172] Compared with the traditional electric gripper, the structure of this embodiment has a simple overall structure, low manufacturing cost, and occupies less space, which is conducive to improving the space utilization rate of the entire equipment.

[0173] Example 9

[0174] The present embodiment further defines the reagent loading mechanism 7 in the embodiment 1. As shown in FIGS. 28-33 , the reagent loading mechanism 707 of the present embodiment includes a cylindrical device housing, a reagent reagent turntable 712 disposed in the device housing, reagent reagents 704 arranged in a circular array on the reagent reagent turntable 712, a pressing device disposed on the device housing, and an air inlet connector 711 and a liquid outlet electromagnetic switch 710 disposed on the bottom surface of the device housing. An air inlet pipe 705 corresponding to the air inlet connector 711 and a nozzle 709 corresponding to the liquid outlet electromagnetic switch 710 are provided at the bottom of the reagent reagent 704.

[0175] The reagent loading mechanism 7 is arranged above one side of the reaction disk mechanism 6 and can correspond to the reaction cup on the reaction disk mechanism 6 by rotating. The nozzle 709 is opposite to the corresponding reaction cup and directly sprays the reagent into the reaction cup.

[0176] The air intake pipe 705 is provided with a sealing spring 706, a sealing gasket 713 and a rubber plug 707 from top to bottom. A through hole is provided at the center of the rubber plug 707 for cooperating with the air intake connector 711 to achieve air intake. In a natural state, the sealing spring 706 presses the sealing gasket 713 against the rubber plug 707 to block the through hole on the rubber plug 707 to achieve sealing. When the test kit 704 moves downward under the action of the downward pressure device, the air intake connector 711 is inserted into the rubber plug 707 to lift up the sealing gasket 713 to achieve connectivity.

[0177] Preferably, the through hole on the rubber stopper 707 is tapered, and the air inlet connector 711 has a corresponding shape.

[0178] Preferably, the device housing includes a reagent chamber 702 and a chamber cover 701 covering the reagent chamber 702. A vertical slide groove is provided in the reagent chamber 702, and the reagent chamber 704 is correspondingly arranged in the slide groove, and a return spring 708 is provided between the reagent chamber 704 and the reagent chamber turntable 712.

[0179] A plurality of first air guide grooves 714 are formed on the top side of the air inlet connector 711 , and a plurality of second air guide grooves 715 are formed on the side wall of the sealing gasket 713 , so that the gas can smoothly enter the reagent kit 704 after the air inlet connector 711 pushes the sealing gasket 713 away.

[0180] The pressing device is a screw motor 703.

[0181] When the present embodiment is in use, the reagent box 704 is placed on the reagent box turntable 712 and can be rotated to the liquid adding position. There is a fixed reagent box pressing device on the upper part, and an air inlet connector 711 and a liquid outlet electromagnetic switch 710 on the lower part. After the reagent box is rotated to the liquid adding position, the pressing device presses the reagent box 704 downward, and the rubber plug 707 on the reagent box contacts and seals with the air inlet connector. The upper part of the air inlet connector 711 lifts the sealing gasket 713 inside the air inlet pipe 705, and the interior of the reagent box is inflated through the air inlet connector 711; the nozzle 709 of the reagent box is controlled by the electromagnetic switch to spray the reagent outward; after the liquid spraying is completed, the pressing device moves upward, and the reagent box 704 moves upward under the action of the return spring 708, so that the reagent box is separated from the air inlet connector 711 and the liquid outlet electromagnetic switch 710, and the sealing gasket 713 inside the air inlet pipe 705 presses the rubber plug 707 under the action of the sealing spring 706 to seal the reagent box; then the next reagent box is rotated to the liquid adding position for liquid addition. There will be no contamination problem during the entire spraying process. For this reason, a variety of test kits with different reagents can be set up to achieve a variety of detection projects.

[0182] Example 10

[0183] As a further improvement of the present invention, this embodiment also includes a reaction cup throwing robot arm 11, and a reaction cup throwing channel 12 is provided on the base 1. The reaction cup throwing robot arm 11 is used to move the used reaction cups on the reaction cup plate mechanism 6 to the reaction cup throwing channel 12.

[0184] At the same time, a chemiluminescence detection mechanism 13 is also provided on the running track of the reaction cup throwing robot arm 11, and the reaction cup throwing robot arm 11 can pick up the reaction cup to the workstation for chemiluminescence detection.

[0185] The overall working process of the present invention is:

[0186] The whole machine operation process of the present invention is:

[0187] 1. The test tube rack 10 transports the test tube to the bottom of the sampling mechanical arm 5 through the test tube rack transport device 2. The sampling mechanical arm takes samples. If the whole blood sample can be tested, the whole blood can be directly added to the reaction cup of the reaction disk mechanism 6. If the whole blood sample needs to be centrifuged to remove plasma before the plasma can be tested, the whole blood is added to the centrifugal disk of the blood separation mechanism 8, and the plasma is obtained by centrifugation. The sampling mechanical arm 5 then extracts the plasma and adds the plasma to the reaction cup of the reaction disk mechanism 6. The reaction cup is rotated by the reaction disk mechanism 6, and the reaction cup is rotated to the bottom of the reagent loading mechanism 7 according to the reaction time requirement, and the reagent is added. The reaction cup is mixed by the mixing mechanism on the reaction disk mechanism 6. Wait until the detection time to rotate the reaction cup to the optical path detection mechanism station for detection. After the detection is completed, the reaction cup is rotated to the bottom of the reaction cup upper cup mechanical arm 4, and the waste liquid is extracted by the liquid aspiration needle on the mechanical arm, and then the reaction cup is grabbed and moved to the reaction cup throwing channel position, and the cup is thrown. The reaction cup enters the reaction waste cup collection box on the lower layer of the base 1. A detection operation process is completed.

[0188] 2. If the sample to be tested needs to be tested by chemiluminescence detection, after adding the reagent to the reaction cup, the reaction cup throwing cup robot arm 11 moves the reaction cup to the chemiluminescence detection mechanism 13 station for detection. After completion, the reaction cup throwing cup robot arm 11 extracts the waste liquid, moves the reaction cup to the reaction cup throwing cup channel position, throws the cup, and the reaction cup enters the reaction waste cup collection box. A detection operation process is completed.

[0189] 3. In the blood separation mechanism 8, the centrifugal disc is pre-stored. Through the operation of the mechanism, the centrifugal disc moves to the centrifugal station, and starts centrifugation after adding blood, centrifuges out plasma, and is extracted. The centrifugal disc will be moved to the throwing disc position and thrown out, and the centrifugal waste disc enters the centrifugal waste disc collection box.

[0190] During the operation of the whole machine of the present invention, the various components operate in a coordinated and unified manner. Through the synchronous operation of the various components, the detection efficiency is greatly improved, and the detection throughput can reach 280 test / h (280 test results per hour).

[0191] At present, the technical solution of the present invention has been put into pilot production, that is, a small-scale test of the product before large-scale mass production; after the pilot production was completed, a user usage survey was carried out in a small range, and the survey results showed that user satisfaction was high; now preparations have begun for the formal production of the product for industrialization (including intellectual property risk warning survey).

[0192] The above embodiments are only used to illustrate the technical solution of the present invention, but not to limit it;

[0193] The foregoing embodiments have described the present invention in detail. Those skilled in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or make equivalent substitutions for some of the technical features therein; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A protein analyzer, characterized in that It comprises a base (1), a test tube rack transport device (2) arranged on the base (1), a sampling mechanical arm (5) arranged on one side of the test tube rack transport device (2) and used for sucking samples in a test tube, a reaction cup plate mechanism (6) arranged at a corresponding position at the other end of the sampling mechanical arm (5), a reaction cup loading mechanism (3) arranged on one side of the reaction cup plate mechanism (6), a reaction cup loading mechanical arm (4) arranged between the reaction cup loading mechanism (3) and the reaction cup plate mechanism (6), and a reagent loading mechanism (7) arranged on one side of the reaction cup plate mechanism (6); A blood separation mechanism (8) is provided between the test tube rack transport device (2) and the reaction cup tray mechanism (6), the blood separation mechanism (8) comprising a centrifugal disk (806), a transfer tray (812), and a centrifugal drive device arranged on one side of the transfer tray (812); an electromagnet (814) is provided above the transfer tray (812), and a slideway (815) is provided below the transfer tray (812) and at a position corresponding to the electromagnet (814); a base plate (816) with an opening is provided at the bottom end of the slideway (815); The centrifugal drive device comprises a centrifugal drive motor (818) and a centrifugal chuck (820) arranged on the output shaft of the centrifugal drive motor (818); a through hole for the centrifugal chuck (820) to pass through is arranged on the transfer tray (812); the centrifugal disk (806) cooperates with the centrifugal chuck (820); and a lifting device for moving the centrifugal disk up and down is also arranged on the centrifugal drive device; a receiving barrel (807) for accommodating the centrifugal disk is arranged on the other side of the transfer tray (812); a slot hole is opened at the bottom of the receiving barrel (807) and on one side corresponding to the transfer tray (812); and a push plate (804) corresponding to the position of the slot hole is arranged on the other side of the receiving barrel (807); the push plate (804) is slidably arranged on the third guide rod (805) through a third slider (803); the third slider (803) is fixedly connected to a third synchronous belt (802) driven by a third displacement drive motor (801); the height of the slot hole is higher than the thickness of one centrifugal disk and lower than the thickness of two centrifugal disks; A first gear (819) is sleeved on the output shaft of the centrifugal drive motor (818), and a second gear (822) is provided on one side of the centrifugal drive motor (818). The second gear (822) is arranged on the output shaft of a stepper motor (823). The stepper motor (823) is connected to a displacement drive device, and the displacement drive device drives the second gear (822) to move so as to engage with or disengage from the first gear (819). The displacement drive device comprises a first synchronous belt (826) and a first displacement drive motor (824) for driving the first synchronous belt (826) to move. The stepper motor (823) is fixedly mounted on a first slider (825), and the first slider (825) is slidably arranged on a first guide rod (827). The first slider (825) is fixedly connected to the first synchronous belt (826). When the transfer tray (812) moves to the bottom of the electromagnet (814) with the centrifugal disk (806) after centrifugation, the extended end of the electromagnet (814) is inserted into the central hole of the centrifugal disk (806), and the insertion depth is less than the thickness of the centrifugal disk (806). When the transfer tray (812) continues to move, the centrifugal disk (806) is blocked by the extended end of the electromagnet (814), and the centrifugal disk (806) can no longer follow the transfer tray (812) to continue to move forward. When the centrifugal disk (806) escapes from the transfer tray (812), it falls into the slideway (815) below, and then falls into the centrifugal waste disk collection box on the lower layer of the base (1); at the same time, the electromagnet (814) is turned off, and the centrifugal disk (806) continues to fall along the slideway (815) to the opening of the base plate (816), and the disk withdrawal action is completed.

2. A protein analyzer according to claim 1, characterized in that The reaction cup and disk mechanism (6) comprises a base (601), a constant temperature ring (603) arranged on the base (601), a reaction disk (602) located inside the constant temperature ring (603) and rotatably arranged on the base (601), a reaction cup (302) placed on the reaction disk (602), a mixing device arranged below the reaction disk (602) and corresponding to the position of the reaction cup (302), and an optical path detection mechanism arranged on one side of the reaction disk (602); a plurality of placement grooves are arranged at the edge of the reaction disk (602), and all the placement grooves form a ring shape and are concentric with the rotation center of the reaction disk (602).

3. A protein analyzer according to claim 1, characterized in that The reagent loading mechanism (7) is arranged at an upper position on one side of the reaction cup plate mechanism (6), and comprises a device housing, a reagent kit turntable (712) arranged in the device housing, reagent kits (704) arranged in a circular array on the reagent kit turntable (712), a return spring (708) arranged between the reagent kit turntable (712) and the reagent kit (704), and a pressing device arranged on the device housing for pressing down the reagent kit (704); An air inlet connector (711) and a liquid outlet electromagnetic switch (710) are respectively provided at the bottom of the device housing, and an air inlet pipeline (705) and a nozzle (709) are correspondingly provided at the bottom of the reagent box (704); when the reagent box (704) is pressed down, the air inlet connector (711) is connected to the air inlet pipeline (705), and the nozzle (709) is connected to the liquid outlet electromagnetic switch (710); A rubber plug (707) is provided in the air intake pipe (705), a sealing gasket (713) is provided above the rubber plug (707), a sealing spring (706) is provided between the sealing gasket (713) and the top of the air intake pipe (705), and a through hole matching the air intake joint (711) is provided on the rubber plug (707). After the test kit (704) is pressed down, the air intake joint (711) lifts up the sealing gasket (713).

4. A protein analyzer according to claim 1, characterized in that: The reaction cup upper cup mechanism (3) comprises a fixing plate (313), a reaction cup upper cup plate (301) arranged on the fixing plate (313), a guide rail (305) installed on the fixing plate (313) and located at one side of the bottom of the reaction cup upper cup plate (301), a tearing tape fixing shaft (304) arranged above the guide rail (305), and a tape reel (308) arranged above the tearing tape fixing shaft (304); a plurality of turns of reaction cup tape (306) are wound in the reaction cup upper cup plate (301); the other end of the reaction cup tape (306) is connected to the tape reel (308) after passing the tearing tape fixing shaft (304) from below; a reaction cup (302) is installed on the reaction cup tape (306); two fins at the top of the reaction cup (302) are provided with snap-fitting protrusions (322), and snap-fitting holes corresponding thereto are opened on the reaction cup tape (306).

5. A protein analyzer according to claim 4, characterized in that A reaction cup groove is provided on the guide rail (305), and the cup body of the reaction cup (302) can enter the reaction cup groove. Reaction cup blocking pieces (316) are provided on the left and right sides above the reaction cup groove. A rectangular groove is formed between the reaction cup blocking piece (316) and the guide rail (305). The extension direction of the rectangular groove is parallel to the moving direction of the reaction cup. The two fins on the top of the reaction cup (302) are located in the corresponding rectangular groove.

6. A protein analyzer according to claim 4, characterized in that A rotating shaft (320) is provided on the fixing plate (313), a cup plate reel (310) is provided in the cup plate (301) on the reaction cup, and one end of the reaction cup belt (306) is connected to the cup plate reel (310); three L-shaped metal springs (319) arranged in a triangular shape are provided in the cup plate reel (310), and the cross section of the rotating shaft (320) is approximately triangular, and an oblong hole is provided at one end of the metal spring (319), and the metal spring (319) is connected to the corresponding edge on the rotating shaft (320) by arranging a bolt in the oblong hole, and the other end of the metal spring (319) is in contact with the cup plate reel (310).

7. A protein analyzer according to claim 1, characterized in that The sampling mechanical arm (5) comprises a moving arm, a mounting seat (501) arranged on the moving arm, a sampling needle (502) arranged on the mounting seat (501), a needle washing assembly (511) arranged at the bottom of the mounting seat (501), and a needle washing pipeline connected to the needle washing assembly (511); a sampling pipeline (515) is connected to the top of the sampling needle (502), and a plunger pump (504) is provided on the sampling pipeline (515); the needle washing assembly (511) comprises a needle washing seat (516), a cleaning chamber (517) arranged inside the needle washing seat (516), and a A water inlet hole (518) and a water absorption hole (519) are connected to the cleaning chamber (517); sealing holes (520) are respectively provided on the needle washing seat (516) and located at the upper and lower ends of the cleaning chamber (517); the sampling needle (502) passes through the sealing hole (520) at the upper end and enters the cleaning chamber (517); the needle washing pipeline is connected to the water inlet hole (518) and the water absorption hole (519); an up-and-down moving arm (521) is provided on the mounting seat (501); the upper end of the sampling needle (502) is fixed on the up-and-down moving arm (521) and moves up and down with it.

8. A protein analyzer according to claim 7, characterized in that The needle washing pipeline comprises a water suction pipeline (514) connected to a water suction hole (519) and a water inlet pipeline (513) connected to a water inlet hole (518); a liquid suction pump (510) is provided on the water suction pipeline (514), and a waste water bucket (509) is provided at the end of the water suction pipeline (514); two paths are provided in parallel on the water inlet pipeline (513), one path is connected to a cleaning liquid bucket (506) and is provided with a first peristaltic pump (508), and the other path is connected to a pure water bucket (505) and is provided with a second peristaltic pump (507); an electromagnetic switching valve (503) is provided on the sampling pipeline (515), and one end of the water inlet pipeline (513) is connected to the electromagnetic switching valve (503).

Images