Powdery mildew inoculation device and powdery mildew rapid batch inoculation method

Abstract

The invention provides a powdery mildew inoculation device and a powdery mildew rapid batch inoculation method. The powdery mildew rapid inoculation device comprises an outer box, an inner box, a porous rotating platform, an adjustable speed circulating fan and a plurality of inoculum fixing frames; the adjustable speed circulating fan is fixedly installed on the top of the inner box, four inoculum fixing frames are installed on the top of the inner box, and the porous rotating platform is installed on the bottom of the inner box. When inoculating powdery mildew, the inoculum is placed on the inoculum fixing frame, the plants to be inoculated are placed on the porous rotating platform, the inner and outer box doors are closed, the porous rotating platform is started, the adjustable speed circulating fan is turned on, the powdery mildew conidia attached to the leaf surface of the inoculum are blown off, and are attached to the wetted plants to be inoculated with the air flow, and the conidia that are not attached continue to reach the adjustable speed circulating fan with the air flow through the ventilation duct, and a new round of inoculation begins. Through the device and the inoculation method, powdery mildew can be inoculated quickly and in batches.

Description

Technical Field

[0001] The invention belongs to the technical field of plant protection, and in particular relates to a powdery mildew inoculation device and a powdery mildew rapid batch inoculation method. Background Art

[0002] Powdery mildew is one of the common fungal diseases in the world. Nearly 10,000 angiosperms have been reported as hosts, most of which are dicotyledons. The disease can occur from the seedling stage to the adult plant, mainly harming leaves, petioles and stems. During the onset period, the powdery mildew fungus grows exponentially and spreads rapidly, causing large areas of plant leaves to die, resulting in a sharp decline in yield and quality, and causing significant economic losses. Powdery mildew is a disease that is spread by airflow, mainly through the conidia of powdery mildew with airflow, and the fungus cannot grow as an obligate parasite on the culture medium, and can only be transferred from generation to generation on fresh living host plants. Therefore, when studying the occurrence of diseases, identifying the disease resistance of varieties, and determining the biological activity of pesticides, artificial inoculation is required to draw more reliable conclusions. The methods for identifying powdery mildew resistance mainly include field natural disease identification method and indoor seedling artificial inoculation identification method. The natural environment in the field is relatively complex and there are uncontrollable factors, which may affect the occurrence and symptom expression of powdery mildew. At present, the indoor seedling artificial inoculation method has been widely used in powdery mildew resistance identification. The main methods of powdery mildew inoculation include spore suspension spray, leaf brushing, and dry powder shaking. It has been reported that 13 powdery mildew species, including cucumber, pumpkin, watermelon, gourd, pepper, tomato, strawberry, grape, apple, melon, rubber tree, tobacco, and rose, can be evaluated for resistance by spore suspension spray and dry powder shaking. For example, Zhang Zhihong et al. used spray and shake-off methods to identify strawberry powdery mildew resistance. The results showed that the mycelium grew very well and was evenly distributed when inoculated by spray. When inoculated by artificial shaking, the mycelium also grew very well, but the mycelium distribution on the leaf disk was not as uniform as that by spray. The inoculation amount can only be estimated based on the experience of the inoculator, which may affect the identification results. Zhang Ruowei et al. used thin-skinned melon inbred lines as test materials, and compared the inoculation methods such as spraying, leaf brushing, and rubbing. The best inoculation method was spraying. However, there are some powdery mildew species, such as wheat, barley, rose, pea, and alfalfa, which cannot be sprayed with spore suspension, but can only be inoculated with spore powder. This is mainly because the conidia of these powdery mildew can maintain the same shape within 6-8 hours in the spore suspension prepared with sterile water, and will burst and die after 10 hours due to water absorption. In order to solve the shortcomings of spore powder inoculation methods such as leaf smearing and dry powder shaking, such as time-consuming and labor-intensive, poor inoculation uniformity and low inoculation efficiency, a powdery mildew inoculation device and a powdery mildew rapid batch inoculation method were formed through experimental improvements. Summary of the invention

[0003] The present invention aims to solve the above technical problems and provides a powdery mildew inoculation device and a powdery mildew rapid batch inoculation method.

[0004] In order to achieve the above object, the present invention adopts the following technical solution:

[0005] A powdery mildew rapid inoculation device comprises an outer box, an inner box, a porous rotating platform, an adjustable speed circulating fan and a plurality of inoculum fixing frames; the inner box is arranged in the outer box, and a gap is provided between the inner box and the outer box to form a ventilation duct, the adjustable speed circulating fan is fixedly mounted on the top of the inner box, and the top of the inner box is detachably provided with the plurality of inoculum fixing frames around the adjustable speed circulating fan, the porous rotating platform is installed at the bottom of the inner box, the porous rotating platform is used for placing plants to be inoculated, the bottom of the inner box is provided with an opening connected to the ventilation duct, and the porous rotating platform is connected to an adjustable speed rotating motor.

[0006] Preferably, the outer box body is rotatably connected to an outer box body door, and an inner box body door is fixedly provided on the outer box body door.

[0007] A powdery mildew rapid batch inoculation method based on the powdery mildew rapid inoculation device comprises the following steps:

[0008] (1) Preparation of powdery mildew inoculum:

[0009] Pick diseased leaves that are severely infected with powdery mildew in the field;

[0010] Shake off powdery mildew conidia from diseased leaves - on inoculum plants with young leaves;

[0011] The inoculated potted inoculum was kept in an isolated growth room and trained to make these leaves surface powdery mildew fungi;

[0012] (2) Preparation of plants to be inoculated:

[0013] Transplant the plants to be inoculated into nutrient pots, carry out cultivation management, and allow the plants to grow normally, and cultivate the plants to be inoculated to the seedling standard;

[0014] (3) Rapid batch inoculation of powdery mildew:

[0015] Place the inoculum on the inoculum fixing rack at the top of the inner box, spray the plants to be inoculated, and place them on the porous rotating platform at the bottom of the inner box, leaving a certain gap between the plants, close the inner box door and the outer box door, start the adjustable speed rotating motor, turn on the adjustable speed circulating fan, and the powdery mildew conidia attached to the leaf surface of the inoculum will be blown off and spread and attached to the sprayed plants to be inoculated with the air flow. The conidia that are not attached will continue to follow the air flow through the ventilation duct to the adjustable speed circulating fan, and a new round of inoculation will begin. Preferably, in step (1), the inoculated potted inoculum is stored in an isolated growth room, and 14 hours of lighting is provided every day, with a light intensity of 100±10µmol.m -2 s -1 , temperature is 25±3℃, humidity is 85%±10%, and culture is carried out for 7-14 days.

[0016] Preferably, in step (3), the rotation speed of the adjustable speed circulating fan is 1.2-3.5 m / s, and the air volume of the adjustable speed circulating fan is 60-150 m³ / h.

[0017] Preferably, in step (3), the speed of the adjustable speed rotating motor is 5-10 r / min.

[0018] (4) Disinfection and sterilization of inoculation device:

[0019] After the same batch of powdery mildew inoculation is completed, open the inner and outer box doors to take out all the plants inside, spray the inner box, porous rotating platform, adjustable speed circulating fan, inoculum fixing frame and ventilation duct inside the inoculation device with 70%~75% alcohol, evenly spray and close the inner and outer box doors, open the porous rotating platform and circulating fan for 2-10 minutes, and then open the inner and outer box doors to continue running for 2-10 minutes. After the inoculation device is disinfected and sterilized and the alcohol evaporates, the next batch of inoculation can be carried out.

[0020] Preferably, in step (4), 72% medical alcohol can be used.

[0021] Preferably, in step (4), the porous rotating platform and the circulating fan are turned on and run for 4-6 minutes, and then the inner and outer box doors are opened and the operation is continued for 4-6 minutes. After adopting the above technical solution, the present invention has the following advantages:

[0022] The powdery mildew rapid inoculation device and the powdery mildew rapid batch inoculation method can be used to rapidly and batch-wise inoculate powdery mildew. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the structure of a powdery mildew rapid inoculation device;

[0024] Figure 2It is a schematic diagram of the working principle of a powdery mildew rapid inoculation device;

[0025] Figure 3 It is a schematic diagram of the connection structure between the inner box and the porous rotating platform;

[0026] In the figure:

[0027] 1-outer box; 101-outer box door; 2-inner box; 201-inner box door; 3-multi-hole rotating platform; 4-adjustable speed circulation fan; 5-inoculum fixing frame; 6-ventilation duct; 7-adjustable speed rotating motor. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0029] like Figure 1-3 As shown, a powdery mildew rapid inoculation device comprises an outer box 1, an inner box 2, a porous rotating platform 3, an adjustable speed circulating fan 4 and a plurality of inoculum fixing frames 5.

[0030] The inner box 2 is arranged in the outer box 1, and a gap is provided between the inner box 2 and the outer box 1 to form a ventilation passage 6. The outer box 1 is in a rectangular parallelepiped shape, and the inner box 2 is in a cylindrical shape. The outer box 1 is rotatably connected to an outer box door 101, and an inner box door 201 is fixedly provided on the outer box door 101. The front of the inner box 2 is provided with an opening matching the inner box door 201. The inner box door 201 is in an arc shape, and the middle part of the inner box door 201 is fixedly connected to the inner side of the outer box door 101 by fixing screws. After the outer box door 101 is closed, the inner box door 201 is buckled with the inner box 2.

[0031] The adjustable speed circulation fan 4 is fixedly mounted on the top of the inner box 2, and the top of the inner box 2 is detachably mounted with a plurality of inoculum fixing frames 5 around the adjustable speed circulation fan 4. In this embodiment, there are four inoculum fixing frames 5, and the four inoculum fixing frames 5 are evenly arranged around the adjustable speed circulation fan 4. Figure 3 The opening in the middle of the top of the inner box is used to install the circulation motor 4, and four screw holes are evenly arranged around the circulation motor 4 for installing the inoculum fixing frame 5.

[0032] The porous rotating platform 3 is installed at the bottom of the inner box 2. The porous rotating platform 3 is used to place the plants that need to be inoculated. The bottom of the inner box 2 is provided with an opening connected to the ventilation duct 6. The porous rotating platform 3 is connected to an adjustable speed rotating motor 7.

[0033] The porous rotating platform 3 is provided with a plurality of through holes, which are used to form a circulating ventilation channel, so that the powdery mildew spores enter the ventilation channel through the through holes on the porous rotating platform 3, and a new round of inoculation begins. The high void ratio of the porous rotating platform 3 can prevent the powdery mildew spores from sticking and accumulating on it.

[0034] The wind circulation direction in the ventilation duct 6 starts from the adjustable speed circulation fan 4, and blows the powdery mildew conidia to the inoculated plants when passing through the inoculum. The airflow flows from the porous rotating platform 3 at the bottom of the inner box 2 to the ventilation duct 6 around the outer box 1 and the inner box 2 and returns to the top of the inner box 2. When the airflow is accelerated again by the adjustable speed circulation fan 4, a new inoculation starts.

[0035] A powdery mildew rapid batch inoculation method based on the powdery mildew rapid inoculation device comprises the following steps:

[0036] (1) Preparation of powdery mildew inoculum:

[0037] Pick diseased leaves infected with powdery mildew in the field;

[0038] Shake off powdery mildew conidia from diseased leaves - on inoculum plants with young leaves;

[0039] The inoculated potted inoculum was kept in an isolated growth chamber and provided with 14 h of lighting per day at a light intensity of 100 ± 10 µmol.m -2 s -1 , the temperature is 25±3℃, the humidity is 85%±10%, and after 7-14 days of training, the powdery mildew on the surface of these leaves is killed. The temperature during the day is 25-28℃, the humidity is 75%-85%; the temperature at night is 23-25℃, and the humidity is 85%-95%.

[0040] (2) Preparation of plants to be inoculated:

[0041] Transplant the plants that need to be inoculated into nutrient pots, carry out cultivation management, allow the plants to grow normally, and cultivate the plants that need to be inoculated to the seedling standard.

[0042] (3) Rapid batch inoculation of powdery mildew:

[0043] The inoculum is placed on the inoculum fixing frame 5 at the top of the inner box 2, the plants to be inoculated are sprayed and placed on the porous rotating platform 3 at the bottom of the inner box 2, leaving a certain gap between the plants, the adjustable speed rotating motor 7 is started, and the adjustable speed circulating fan 4 is turned on, the powdery mildew conidia attached to the leaf surface of the inoculum are blown off, and spread and attached to the sprayed wet plants to be inoculated with the air flow, and the unattached conidia continue to pass through the ventilation duct 6 with the air flow to the adjustable speed circulating fan 4, and a new round of inoculation begins. After the inoculation is completed, the plants are taken out, and new plants to be inoculated are placed, and the inoculum is replaced as appropriate, and the inoculation is restarted.

[0044] The speeds of the above-mentioned adjustable speed circulation fan 4 and adjustable speed rotating motor 7 are adjustable. Specifically, the speed of the adjustable speed circulation fan 4 is 1.2-3.5m / s, and the air volume of the adjustable speed circulation fan is 60-150m³ / h; the speed of the adjustable speed rotating motor 5 is 5.0-10.0r / min.

[0045] (4) Disinfection and sterilization of inoculation device:

[0046] After the same batch of powdery mildew inoculation is completed, the inoculation device is sterilized with 70%~75% alcohol, and then the porous rotating platform and circulating fan are turned on for several minutes to promote disinfection and alcohol volatilization. After completion, the next batch of inoculation can be carried out.

[0047] (5) Management of plants after inoculation.

[0048] The inoculated plants are placed at a temperature of 23-28°C and a humidity of 75%-95%, provided with appropriate light, and can be observed and counted after 3-14 days of cultivation.

[0049] By means of the powdery mildew rapid inoculation device and the powdery mildew rapid batch inoculation method, powdery mildew can be inoculated rapidly and in batches.

[0050] Example 1: Rapid batch inoculation of strawberry powdery mildew.

[0051] (1) Preparation of strawberry powdery mildew inoculum:

[0052] The experimental variety was Red Cheek, which was not resistant to powdery mildew. On September 1, the Red Cheek strawberry seedlings were transplanted into 10*10cm nutrient pots. New leaves grew after routine management. On October 8, leaves seriously infected with powdery mildew were picked in the field, and the powdery mildew conidia on the diseased leaves were shaken off onto the strawberry seedlings prepared for transplanting. The strawberry seedlings inoculated with powdery mildew were placed on an indoor culture rack and provided with 14 hours of lighting per day, with a light intensity of 100±10µmol.m -2 s -1Around 25-28℃ during the day, 75%-85% humidity. 22-25℃ at night, 85%-95% humidity. 7 days after inoculation, small powdery mildew spots appeared on the leaves, and 14 days later, the leaves were covered with powdery mildew spots, and the inoculum was ready.

[0053] (2) Preparation of plants to be inoculated:

[0054] On September 1, the red cheek strawberry seedlings that need to be inoculated will be transplanted into nutrient pots and routine management will be carried out to ensure the normal growth of the plants and the emergence of new leaves.

[0055] (3) Rapid batch inoculation of powdery mildew:

[0056] On October 20, four inoculum strawberry seedlings were placed on the inoculum fixing frame at the top of the inner box. The strawberry plants to be inoculated were sprayed wet and placed on the porous rotating platform at the bottom of the inner box, leaving a certain gap between the plants. About 50 strawberries can be placed on the porous rotating platform with a diameter of 95 cm. Close the inner and outer box doors, start the adjustable speed rotary motor, and the speed of the porous rotating platform is 8r / min. Turn on the adjustable speed circulating fan, and the wind speed is 2 m / s. The powdery mildew conidia attached to the leaf surface of the inoculum are blown off, and spread and attached to the wetted plants to be inoculated with the air flow. The unattached conidia continue to pass through the ventilation duct with the air flow to the adjustable speed circulating fan, and a new round of inoculation begins. After starting the inoculation for 1 minute, turn off the adjustable speed circulating fan and the adjustable speed rotating motor, open the box door and take out the inoculated strawberry plants. Replace the inoculum strawberry, re-place the strawberries to be inoculated, and start a new batch of inoculation.

[0057] (4) Disinfection and sterilization of inoculation device:

[0058] After all the strawberry powdery mildew inoculations for this batch are completed, spray the inner box, porous rotating platform, adjustable speed circulating fan, inoculum fixing frame and ventilation duct inside the inoculation device with 72% alcohol. After evenly spraying, close the inner and outer box doors, open the porous rotating platform and circulating fan to run for 5r / min, and then open the inner and outer box doors to continue running for 5min. After the inoculation device is disinfected and sterilized and the alcohol evaporates, it is ready for use.

[0059] (5) Management after plant inoculation:

[0060] The inoculated strawberry seedlings were placed on an indoor culture rack and provided with 14 h of lighting per day at a light intensity of 100 ± 10 µmol.m -2 s -1 Around 2000, the daytime temperature is 25-28℃, and the humidity is 75%-85%. The night temperature is 22-25℃, and the humidity is 85%-95%. After 7 days, small powdery mildew spots appear on the leaves, and the incidence rate is counted and the disease index is calculated.

[0061] (6) Verification of vaccination effect:

[0062] Three batches were inoculated rapidly, with each batch as a replicate. Spraying of spore suspension was used as control, and the conidia inoculation concentration was 1X10 6 .

[0063] Investigate the leaves of 50 plants in each repetition, and grade them according to the percentage of the lesion area on each leaf to the entire leaf. Level 0: Leaves are disease-free; Level 1: The lesion area accounts for 5% or less of the entire leaf; Level 3: The lesion area accounts for 6% to 15% of the entire leaf area; Level 5: The lesion area accounts for 16% to 25% of the entire leaf area; Level 7: The lesion area accounts for 26% to 50% of the entire leaf area; Level 9: The lesion area accounts for more than 50% of the entire leaf area. Disease index (%) = ∑ (number of diseased plants at each level × representative value of each level) / (total number of plants investigated × highest representative value) × 100.

[0064] deal with Incidence rate (%) Disease index (%) Vaccination effect Inoculation speed Rapid batch inoculation 1 78.6±4.2 36.4±2.4 good quick Rapid batch inoculation 2 82.5±5.4 41.1±3.1 good quick Rapid batch inoculation 3 81.4±3.7 40.5±2.2 good quick Spore suspension spray 85.4±3.4 46.3±3.2 good Faster

[0065] Based on the above results, the rapid batch inoculation and spore suspension spray inoculation have equivalent effects.

[0066] Example 2: Rapid batch inoculation of gourd powdery mildew.

[0067] (1) Preparation of gourd powdery mildew inoculum:

[0068] The test variety is Hangzhou gourd, which is not resistant to powdery mildew. On March 5, the Hangzhou gourd seeds were soaked and germinated, and then directly planted into 8*8cm nutrient pots and placed on the culture rack. The temperature was controlled at 25~28℃, and the seedlings emerged in March. After 30 days of routine management, the seeds reached 2 leaves and 1 heart. In the morning, the diseased leaves of gourd seriously infected with powdery mildew were picked, and the powdery mildew conidia on the diseased leaves were shaken off on the gourd seedlings prepared for transplanting. The gourd seedlings inoculated with powdery mildew were placed on the indoor culture rack, and 12 hours of lighting were provided every day, and the light intensity was 80±10µmol.m -2 s -1 Around 25-28℃ during the day, 75%-85% humidity. 18-23℃ at night, 85%-95% humidity. Small powdery mildew spots appeared on the leaves 5 days after inoculation, and 12 days later, the leaves were covered with powdery mildew spots, and the inoculum was ready.

[0069] (2) Preparation of plants to be inoculated:

[0070] On March 12, the Hangzhou gourd seeds that need to be inoculated will be sown into nutrient pots, and routine management will be carried out to ensure the normal growth of the plants.

[0071] (3) Rapid batch inoculation of powdery mildew:

[0072] On April 13, four inoculated gourd seedlings were placed on the inoculated fixed frame at the top of the inner box, and the gourd plants to be inoculated were sprayed wet and placed on the porous rotating platform at the bottom of the inner box, leaving a certain gap between the plants. About 70 Hangzhou gourd seedlings can be placed on the porous rotating platform with a diameter of 95 cm. Close the inner and outer box doors, start the adjustable speed rotary motor, and the speed of the porous rotating platform is 6r / min. Turn on the adjustable speed circulating fan, and the wind speed is about 1.8 m / s. The powdery mildew conidia attached to the leaf surface of the inoculated body are blown off, and spread and attached to the wetted plants to be inoculated with the air flow. The unattached conidia continue to follow the air flow through the ventilation duct to the adjustable speed circulating fan, and a new round of inoculation begins. After starting the inoculation for 1 minute, turn off the adjustable speed circulating fan and the adjustable speed rotating motor, open the box door and take out the inoculated Hangzhou gourd plants. After replacing the inoculum, re-place the Hangzhou gourd seedlings that need to be inoculated and start a new batch of inoculation.

[0073] (4) Disinfection and sterilization of inoculation device:

[0074] After all the powdery mildew inoculation of this batch of gourd seedlings is completed, the inner box, porous rotating platform, adjustable speed circulating fan, inoculum fixing frame and ventilation duct inside the inoculation device are sprayed with 72% alcohol. After evenly spraying, the inner and outer box doors are closed, the porous rotating platform and the circulating fan are turned on to run for 5r / min, and then the inner and outer box doors are opened to continue running for 5min. After the inoculation device is disinfected and sterilized and the alcohol is evaporated, it is ready for use.

[0075] (5) Management after plant inoculation:

[0076] The inoculated Hangzhou gourd seedlings were placed on the indoor culture rack and provided with 12 h of lighting every day with a light intensity of 80 ± 10 µmol.m -2 s -1 Around 2000 ℃, the daytime temperature is 25-28℃, the humidity is 75%-85%. The night temperature is 20~23℃, the humidity is 85%~95%. After 7 days, a large number of powdery mildew spots appeared on the leaves, and the incidence rate was counted and the disease index was calculated.

[0077] (6) Verification of vaccination effect:

[0078] Three batches were inoculated rapidly, with each batch as a replicate treatment. The shake-off method was used as a control.

[0079] The leaves of 30 plants in each inoculation were investigated, and the lesion area on each leaf was graded according to the percentage of the entire leaf. Grade 0: no symptoms on the leaves; Grade 1: the lesion area on the front of the leaves is less than 30%, and there are no lesions on the back of the leaves; Grade 3: the lesion area on the front of the leaves is greater than 30%, and the lesion area on the back of the leaves is less than 10%; Grade 5: the lesion area on the front of the leaves is greater than 30%, the lesion area on the back of the leaves is greater than 10%, and a small number of lesions appear on the petiole; Grade 7: the front of the leaves is covered with a mold layer, the lesion area on the back of the leaves is greater than 10%, there are more lesions on the petiole, and a small number of lesions appear on the main stem; Grade 9: the front of the leaves is covered with a mold layer, the lesion area on the back of the leaves is greater than 10%, there are more lesions on the petiole, and the main stem is covered with a mold layer. Disease index (%) = ∑ (number of diseased plants at each level × representative value of each level) / (total number of plants investigated × highest representative value) × 100.

[0080] deal with Incidence rate (%) Disease index (%) Vaccination effect Inoculation speed Rapid batch inoculation 1 88.1±4.5 78.9±4.5 good quick Rapid batch inoculation 2 91.3±4.1 85.6±5.3 good quick Rapid batch inoculation 3 83.4±3.7 76.3±3.7 good quick Leaf brush inoculation 75.8±8.6 72.4±7.6 better slow

[0081] Based on the above results, this rapid batch inoculation method can cause disease more evenly than the leaf brushing method and has a faster inoculation speed.

[0082] In addition to the above preferred embodiments, the present invention has other implementation modes. Those skilled in the art may make various changes and modifications based on the present invention. As long as they do not depart from the spirit of the present invention, they should all fall within the scope defined by the claims attached to the present invention.

Claims

1. A method for rapid batch inoculation of powdery mildew, characterized in that The following steps are involved: 1) Preparation of powdery mildew inoculum: Select the same variety as the inoculated plant, and transplant the prepared inoculum plant at a suitable seedling stage into the nutrient pot; Pick diseased leaves seriously infected with powdery mildew in the field, shake off the powdery mildew conidia on the diseased leaves onto the inoculum plants, and complete the powdery mildew inoculation; The inoculated inoculum is cultivated under suitable temperature, humidity and light conditions until the surface of the plant leaves is covered with powdery mildew fungi; The inoculated potted inoculum was kept in an isolated growth chamber and provided with 14 h of lighting per day at a light intensity of 100 ± 10 µmol.m -2 s -1 ; The temperature during the day is 25-28℃, and the humidity is 75%-85%; the temperature at night is 23-25℃, and the humidity is 85%-95%. After 7-14 days of training; 2) Preparation of plants to be inoculated: Transplant the plants that need to be inoculated into nutrient pots and carry out routine management to ensure the normal growth of the plants; 3) Preparation of the inoculation device: The inoculation device includes an outer box, an inner box, a porous rotating inoculation table, an adjustable speed circulating fan and a plurality of inoculation body fixing frames; the inner box is arranged in the outer box, and a ventilation duct is formed with the gap between the inner box and the outer box, but is isolated from the outside world; the adjustable speed circulating fan is fixedly installed on the top of the inner box, and a plurality of detachable inoculation body fixing frames are installed around the ventilation opening of the adjustable speed circulating fan on the top inner wall of the inner box, the porous rotating inoculation table is installed at the bottom of the inner box, and a plurality of through holes are arranged on the porous rotating inoculation table, and the porous rotating inoculation table has a high gap ratio; the porous rotating inoculation table is used to place the plants to be inoculated, and the bottom of the inner box is connected to the ventilation duct through the porous rotating inoculation table, and the porous rotating inoculation table is connected to an adjustable speed rotating motor; the inner box, the porous rotating inoculation table, the circulating fan and the ventilation duct form a closed airflow circulating inoculation space; 4) Rapid batch inoculation of powdery mildew: Place the inoculum on the inoculum fixing rack at the top of the inner box, spray the plants to be inoculated, and place them on the porous rotating inoculation table at the bottom of the inner box, leaving a certain gap between the plants, close the inner box door and the outer box door, start the adjustable speed rotary motor, turn on the adjustable speed circulation fan, the speed of the adjustable speed circulation fan is 1.2-3.5m / s, the air volume of the adjustable speed circulation fan is 60-150m³ / h, and the speed of the adjustable speed rotary motor is 5-10r / min; the powdery mildew conidia attached to the leaf surface of the inoculum are blown off, and spread and attached to the wetted plants to be inoculated with the air flow, and the conidia that are not attached continue to follow the air flow through the ventilation duct to reach the adjustable speed circulation fan, and a new round of inoculation begins; The inoculated plants are placed at a temperature of 23-28°C, a humidity of 75%-95%, and provided with appropriate light. After 3-14 days of cultivation, the statistics can be observed. 5) Disinfection and sterilization of inoculation device: After the same batch of powdery mildew inoculation is completed, open the inner and outer box doors to take out all the plants inside, spray the inner box, porous rotary inoculation table, adjustable speed circulating fan, inoculum fixing frame and ventilation duct inside the inoculation device with 70%-5% alcohol, evenly spray and close the inner and outer box doors, turn on the porous rotary inoculation table and circulating fan for 2-10 minutes, and then open the inner and outer box doors to continue running for 2-10 minutes; The inoculation device is sterilized with 70%-75% alcohol and is ready for use after the alcohol evaporates.

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