Spinal cord hemisection device and hemisection method for making animal spinal cord injury model

Abstract

The application discloses a spinal cord hemisection device and a hemisection method for manufacturing an animal spinal cord injury model. The spinal cord hemisection device comprises a spinal cord hemisection device main body and a master control center. The master control center is arranged outside the spinal cord hemisection device main body and is used for controlling the action of the spinal cord hemisection device main body. The spinal cord hemisection device main body comprises a fixing frame, a hemisection driving mechanism, a spinal cord fixing structure and a cutting assembly. The hemisection driving mechanism is arranged on the fixing frame. The cutting assembly is installed on the hemisection driving mechanism in a lifting manner. After the fixing frame is arranged above a non-human primate to be modeled and a surgical incision of a dura mater is manually opened, the spinal cord fixing structure penetrates into the gap between the spinal cord and the dura mater, surrounds the outer periphery of the spinal cord, the hemisection driving mechanism is located above the incision and drives the cutting assembly to act, and the spinal cord fixing structure performs spinal cord hemisection. The application does not damage too much dura mater and is convenient for postoperative maintenance.

Description

Technical Field

[0001] This invention relates to the field of equipment for creating non-human primate spinal cord injury models, and in particular to a spinal cord hemisection device and hemisection method for creating animal spinal cord injury models. Background Technology

[0002] In medical experiments investigating specific diseases, collecting research data using laboratory animals is a crucial step, such as in studies on spinal cord injuries. Monkeys are particularly suitable laboratory animals for spinal cord injuries, and creating animal models of spinal cord injury using monkeys is a vital component in researching the mechanisms and treatments of spinal cord injury.

[0003] Currently, there are several main animal models of spinal cord injury, including hemostatic forceps-clamped spinal cord crush injury models, heavy object fall-induced spinal cord impact injury models, spinal cord transection injury models, spinal cord hemisection injury models, and balloon compression injury models. However, existing spinal cord hemisection injury models are typically created manually. When manually cutting the spinal cord with a scalpel, it is difficult to control the cutting depth. The spinal cord is surrounded by the dura mater; cutting the dura mater before hemisectioning the spinal cord (severing half of the spinal cord column) easily leads to tearing of the dura mater. Therefore, manual hemisection not only makes it difficult to achieve a smooth hemisection but also makes postoperative maintenance difficult due to excessive tearing of the dura mater. Furthermore, surgical modeling in large animals often requires extremely skilled surgical techniques; spinal cord hemisection modeling is extremely challenging, posing a significant problem for researchers without surgical training. Summary of the Invention

[0004] The purpose of this invention is to provide a spinal cord hemisection device for creating a non-human primate spinal cord injury model. After the device is used and operated skillfully, it can be used percutaneously for spinal cord hemisection, reducing the requirements and reliance on surgical techniques. It is more suitable for researchers who lack surgical expertise and has great potential for scientific research applications.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] A spinal cord hemisection device for creating an animal spinal cord injury model includes a main body and a main control center. The main control center is located outside the main body of the spinal cord hemisection device and is used to control the movement of the main body. The main body of the spinal cord hemisection device includes a fixation frame, a hemisection drive mechanism, a spinal cord fixation structure, and a cutting component. The hemisection drive mechanism is located on the fixation frame, and the cutting component is vertically mounted on the hemisection drive mechanism. After the fixation frame is set above the non-human primate to be modeled and the dura mater is surgically incised, the spinal cord fixation structure penetrates into the gap between the spinal cord and the dura mater and surrounds the periphery of the spinal cord. The hemisection drive mechanism is located above the incision and drives the cutting component to move, and the spinal cord fixation structure hemisections the spinal cord.

[0007] As a further technical solution of the present invention: the half-cut driving mechanism includes a first membrane penetration driving mechanism and a first membrane penetration tube that extends and retracts downward under the drive of the first membrane penetration driving mechanism. The first membrane penetration tube is used to penetrate the dura mater from one side of the gap between adjacent vertebrae.

[0008] As a further technical solution of the present invention: the spinal cord fixation structure includes an insertion soft band and an insertion driving member. One end of the insertion soft band is inserted into the first perforation tube. The insertion driving member is used to drive the insertion soft band to pass through the first perforation tube and extend into the dura mater, and pass through the gap between the dura mater and the spinal cord to the lower periphery of the spinal cord.

[0009] As a further technical solution of the present invention: the cutting assembly includes a second membrane penetration driving mechanism and a second membrane penetration tube that extends and retracts downward under the drive of the second membrane penetration driving mechanism. The second membrane penetration tube is used to penetrate the dura mater from the center of the gap between adjacent vertebrae.

[0010] As a further technical solution of the present invention: the cutting component includes a cutting wire, the cutting wire is attached to the surface of the guide soft strip, the guide soft strip has a sensing section at one end connected to the first membrane tube, one end of the cutting wire extends into the first membrane tube, and the other end is a free end, extending into the sensing section of the guide soft strip.

[0011] As a further technical solution of the present invention: the cutting assembly includes a hook rod, a hook driving component, and a sensing component. The hook rod is vertically and vertically disposed inside the second perforation tube. The hook driving component is used to drive the hook rod to move up and down inside the second perforation tube. The sensing component is installed at the end of the hook rod to cooperate with the sensing section of the guide band. After the sensing component senses that the sensing section has moved directly below the spinal cord, the hook driving component drives the hook rod to extend downward to grasp the free end of the cutting line.

[0012] As a further technical solution of the present invention: the guide strip is provided with a groove for fitting the cutting line, and the groove is provided with an adhesive layer, which fixes the cutting line in the groove.

[0013] As a further technical solution of the present invention: the free end of the cutting line is provided with a magnetic head, and the bottom end of the hook rod is provided with a magnetic suction head. The magnetic suction head attracts the magnetic head, so that the bottom end of the hook rod attracts the free end of the cutting line.

[0014] As a further technical solution of the present invention: the free end of the cutting line is provided with a collar, and the bottom end of the hook rod is provided with a hook. The hook hooks the collar, so that the bottom end of the hook rod hooks the free end of the cutting line.

[0015] Furthermore, the present invention needs to provide a method for hemisection of the spinal cord in non-human primates using the aforementioned spinal cord hemisection device, the cutting method being as follows:

[0016] The spinal cord resection device is used in conjunction with a limiting and fixing mechanism to fix the monkey. The fixing frame is placed above the monkey, and an incision is made manually to expose the spine.

[0017] The hemisection drive mechanism and cutting components are located above the incision. Subsequently, driven by the first perforation drive mechanism, the first perforation tube penetrates the dura mater from the gap between adjacent vertebrae. Driven by the guide drive, the guide band penetrates the dura mater along the first perforation tube and then penetrates the lower part of the spinal cord along the gap between the dura mater and the spinal cord. After the sensor detects that the sensing segment has moved to the lower part of the spinal cord, it transmits a signal to the hook drive. Driven by the second perforation drive mechanism, the second perforation tube penetrates the dura mater from the gap between adjacent vertebrae. The hook drive drives the hook rod to extend downwards in the second perforation tube, penetrate the spinal cord, and grab the free end of the cutting line with the bottom end of the hook rod. The hook drive drives the hook rod to retract, thereby causing the cutting line to detach from the guide band and cut the spinal cord through the cutting line, thus hemisectioning the spinal cord.

[0018] As a further technical solution of the present invention: Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention proposes a spinal cord hemisection device and hemisection method for making animal spinal cord injury models. Through the cooperation between the main body of the spinal cord hemisection device and the main control center, when performing hemisection of the spinal cord, excessive damage to the dura mater is not caused, which facilitates postoperative maintenance. Moreover, the spinal cord hemisection can be flat, so that the non-human primate spinal cord injury models made by the spinal cord hemisection device are consistent. Furthermore, after the device is used and operated skillfully, the spinal cord hemisection operation can be performed percutaneously, reducing the requirements and dependence on surgical techniques. It is more suitable for researchers who lack surgical professional technical training and has great scientific research application prospects. Attached Figure Description

[0019] Figure 1 A schematic diagram of a spinal cord hemisection device for creating a non-human primate spinal cord injury model.

[0020] Figure 2 This is a diagram showing the insertion positions of the first and second membrane-penetrating tubes.

[0021] Figure 3 The first flowchart of a spinal cord hemisection device for creating a non-human primate spinal cord injury model.

[0022] Figure 4 The second flowchart of the spinal cord hemisection device for creating a non-human primate spinal cord injury model.

[0023] Figure 5 The third flowchart of the spinal cord hemisection device for creating a non-human primate spinal cord injury model. Detailed Implementation

[0024] The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit the scope of protection of the present invention.

[0025] Please see Figures 1-5 A spinal cord hemisection device for creating an animal spinal cord injury model includes a spinal cord hemisection device body 10 and a main control center 20. The main control center 20 is located outside the spinal cord hemisection device body 10 to control the movement of the spinal cord hemisection device body 10. The spinal cord hemisection device body 10 includes a fixation frame 11, a hemisection drive mechanism 12, a spinal cord fixation structure 13, and a cutting component 14. The hemisection drive mechanism 12 is mounted on the fixation frame 11, and the cutting component 14 is mounted on the hemisection drive mechanism 12. After the fixation frame 11 is set above the non-human primate to be modeled and the dura mater is surgically cut open, the spinal cord fixation structure 13 penetrates into the gap between the spinal cord and the dura mater and surrounds the spinal cord. The hemisection drive mechanism 12 is located above the incision and drives the cutting component 14 to move, and the spinal cord fixation structure hemisections the spinal cord.

[0026] The half-cutting driving mechanism 12 includes a first membrane penetration driving mechanism 121 and a first membrane penetration tube 122 that can move downward under the drive of the first membrane penetration driving mechanism 121. The first membrane penetration tube 122 can penetrate into the dura mater from the gap between adjacent vertebrae.

[0027] The spinal cord fixation structure 13 includes an insertion soft band 131 and an insertion drive (not shown in the figure). One end of the insertion soft band 131 is inserted into the first perforation tube 122. The end of the insertion soft band 131 connected to the first perforation tube 122 is provided with a sensing section 101. The insertion drive is used to drive the insertion soft band 131 to pass out of the first perforation tube 122 and into the incision, and move along the gap between the dura mater and the spinal cord, along the peripheral wall of the spinal cord, until it penetrates to the lower periphery of the spinal cord.

[0028] The cutting assembly 14 includes a second membrane penetration driving mechanism 141 and a second membrane penetration tube 142 that moves downward under the drive of the second membrane penetration driving mechanism 141. The second membrane penetration tube 142 can penetrate into the dura mater from the gap between adjacent vertebrae. The cutting assembly 14 also includes a cutting wire 132, a hook rod 143, a hook drive (not shown), and a sensing element (not shown). The cutting wire 132 is attached to the surface of the guide band 131 and moves together with the guide band 131. One end of the cutting wire 132 extends into the first membrane penetration tube 122, and the other end extends into the sensing section 101 of the guide band 131. The hook rod 143 is vertically and vertically mounted on the first membrane penetration tube 122. Inside the second perforation tube 142, a hook-and-drive mechanism drives a hook-and-drive rod 143 to move up and down within the tube. A sensor is installed at the end of the hook-and-drive rod 143 to cooperate with the sensing section 101 of the guide band 131. When the sensor detects that the sensing section 101 has moved directly below the spinal cord, it transmits a signal to the hook-and-drive mechanism. The mechanism then drives the hook-and-drive rod 143 to extend downwards within the second perforation tube 142, penetrating the spinal cord. The bottom end of the hook-and-drive rod 143 grasps the free end of the cutting wire 132. The hook-and-drive mechanism then drives the hook-and-drive rod 143 to retract, thereby causing the cutting wire 132 to detach from the guide band 131, and the cutting wire 132 partially severs the spinal cord. Furthermore, the guide band 131 has a groove for attaching the cutting wire 132. An adhesive layer is provided on the groove, which fixes the cutting wire 132 within the groove. When the cutting wire 132 is pulled up, it can break through the adhesive layer.

[0029] Furthermore, the bottom end of the hook rod 143 has a gripping structure that grips the free end of the cutting wire 132, which has various designs. Preferably, two structures are provided: First, the free end of the cutting wire 132 is provided with a magnetic head, and the bottom end of the hook rod 143 is provided with a magnetic suction head. The magnetic suction head attracts the magnetic head, so that the bottom end of the hook rod 143 attracts the free end of the cutting wire 132. After the free end of the cutting wire 132 is pulled up through the spinal cord, the spinal cord is partially cut by external force. Second, the free end of the cutting wire 132 is provided with a collar, and the bottom end of the hook rod 143 is provided with a hook. The hook hooks the collar, so that the bottom end of the hook rod 143 hooks the free end of the cutting wire 132.

[0030] Furthermore, the introducing drive can be disposed on one side of the first membrane penetration drive mechanism 121, including a drive motor, which drives one end of the introducing soft strip 131 to penetrate into the dura mater along the first membrane penetration tube 122.

[0031] Furthermore, the insertion soft band 131 has a certain curvature to facilitate insertion into the lower part of the spinal cord along the gap between the dura mater and the spinal cord.

[0032] It is understood that the spinal cord hemisection method of the spinal cord hemisection device for creating a non-human primate spinal cord injury model of the present invention is as follows: The spinal cord hemisection device is used in conjunction with a limiting and fixing mechanism to fix the monkey. The fixing frame 11 is set above the monkey, and after artificial incision, the spine is exposed. The hemisection driving mechanism 12 and the cutting component 14 are correspondingly located above the incision. Subsequently, driven by the first perforation driving mechanism 121, the first perforation tube 122 penetrates into the dura mater from one side of the gap between adjacent vertebrae. Driven by the introduction driving component, the introduction soft band 131 is inserted into the dura mater along the first perforation tube 122. The first perforation tube 122 penetrates the space between the dura mater and the spinal cord to the lower part of the spinal cord. After the sensing element detects that the sensing segment 101 has moved to the lower part of the spinal cord, it transmits a signal to the hook-and-drive element. Driven by the second perforation driving mechanism 141, the second perforation tube 142 penetrates the dura mater from the space between adjacent vertebrae. The hook-and-drive element drives the hook rod 143 to extend downwards inside the second perforation tube 142, penetrating the spinal cord. The bottom end of the hook rod 143 grabs the free end of the cutting line 132. The hook-and-drive element drives the hook rod 143 to retract, thereby causing the cutting line 132 to detach from the guide band 131 and cut the spinal cord through the cutting line 132, thus partially cutting the spinal cord. After the partial cutting of the spinal cord is completed, the first perforation tube 122 and the second perforation tube 142 retract, cutting the pulled-out cutting line 132. The guide band 131 and the hook rod 143 then retract into the corresponding perforation tubes.

[0033] In summary, the spinal cord hemisection device and hemisection method for creating animal spinal cord injury models of the present invention, through the cooperation between the main body 10 of the spinal cord hemisection device and the main control center 20, does not damage too much of the dura mater when hemisectioning the spinal cord, which facilitates postoperative maintenance. It can also make the spinal cord hemisection flat, so that the non-human primate spinal cord injury models created by the spinal cord hemisection device are consistent. Furthermore, after the device is used and operated skillfully, the spinal cord hemisection operation can be performed percutaneously, reducing the requirements and reliance on surgical skills. It is more suitable for researchers who lack surgical professional skills training and has great scientific research application prospects.

[0034] Any combination of various embodiments of the present invention, provided it does not violate the inventive concept of the present invention, shall be regarded as the content disclosed by the present invention; within the scope of the technical concept of the present invention, any simple modifications to the technical solution and any combination of different embodiments that do not violate the inventive concept of the present invention shall be within the protection scope of the present invention.

Claims

1. A spinal cord hemisection device for creating an animal spinal cord injury model, characterized in that: The device includes a spinal cord hemisection device body (10) and a main control center (20). The main control center (20) is located outside the spinal cord hemisection device body (10) and is used to control the movement of the spinal cord hemisection device body (10). The spinal cord hemisection device body (10) includes a fixation frame (11), a hemisection drive mechanism (12), a spinal cord fixation structure (13), and a cutting component (14). The hemisection drive mechanism (12) is located on the fixation frame (11), and the cutting component (14) is vertically mounted on the hemisection drive mechanism (12). After the fixation frame (11) is set above the non-human primate to be modeled and the dura mater is surgically cut open, the spinal cord fixation structure (13) penetrates into the gap between the spinal cord and the dura mater and surrounds the spinal cord. The hemisection drive mechanism (12) is located above the incision and drives the cutting component (14) to move. The spinal cord fixation structure hemisections the spinal cord. The half-cut driving mechanism (12) includes a first membrane penetration driving mechanism (121) and a first membrane penetration tube (122) that extends and retracts downward under the drive of the first membrane penetration driving mechanism (121). The first membrane penetration tube (122) is used to penetrate the dura mater from one side of the gap between adjacent vertebrae. The spinal cord fixation structure (13) includes an insertion soft band (131) and an insertion drive. One end of the insertion soft band (131) is inserted into the first perforation tube (122). The insertion drive is used to drive the insertion soft band (131) to pass through the first perforation tube (122) and extend into the dura mater, and pass through the gap between the dura mater and the spinal cord to the lower periphery of the spinal cord. The cutting assembly includes a second membrane penetration driving mechanism (141) and a second membrane penetration tube (142) that extends and retracts downward under the drive of the second membrane penetration driving mechanism (141). The second membrane penetration tube (142) is used to penetrate the dura mater from the center of the gap between adjacent vertebrae. The cutting assembly includes a cutting wire (132) which is attached to the surface of the guide strip (131). The guide strip (131) has a sensing section (101) at one end that is connected to the first membrane tube (122). One end of the cutting wire (132) extends into the first membrane tube (122), and the other end is a free end that extends into the sensing section (101) of the guide strip (131). The cutting assembly includes a hook rod (143), a hook drive, and a sensor. The hook rod (143) is vertically and vertically disposed inside the second perforation tube (142). The hook drive is used to drive the hook rod (143) to move up and down inside the second perforation tube (142). The sensor is installed at the end of the hook rod (143) to cooperate with the sensing section (101) of the guide band (131). After the sensor senses that the sensing section (101) has moved to the area directly below the spinal cord, the hook drive drives the hook rod (143) to extend downward to grasp the free end of the cutting line (132).

2. The spinal cord resection device according to claim 1, characterized in that: The guide tape (131) is provided with a groove for attaching the cutting line (132), and the groove is provided with an adhesive layer to fix the cutting line (132) in the groove.

3. The spinal cord resection device according to claim 1, characterized in that: The free end of the cutting line (132) is provided with a magnetic head, and the bottom end of the hook rod (143) is provided with a magnetic head. The magnetic head is attracted by the magnetic head, so that the bottom end of the hook rod (143) is attracted to the free end of the cutting line (132).

4. The spinal cord resection device according to claim 1, characterized in that: The free end of the cutting line (132) is provided with a collar, and the bottom end of the hook rod (143) is provided with a hook. The hook hooks the collar, so that the bottom end of the hook rod (143) hooks the free end of the cutting line (132).

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