Device and method for quantitative collection, processing and storage of liquid samples

Abstract

The present invention discloses a device and method for quantitative collection, processing and preservation of liquid samples, comprising: a quantitative measuring chip and a porous material chip connected to the quantitative measuring chip via a structural member; the quantitative measuring chip is provided with a microchannel network having an inlet and multiple outlets; the porous material chip is provided with multiple processing and preservation units that dock with the multiple outlets when in a folded working state. Utilizing the device of the present invention, quantitative measurement of liquid samples from nanoliter to milliliter levels can be achieved. The present invention combines liquid sample collection, processing and preservation, and immediately performs post-processing such as antibacterial, drying, and enrichment upon completion of liquid sample collection, so that the sample can be preserved on the porous material chip for a long time, greatly facilitating the collection, processing and preservation of liquid samples. The present invention has a simple structure and is easy to operate, and can still maintain reliable performance under non-professional conditions or field conditions.

Description

Technical Field

[0001] The present invention relates to the field of sample collection, processing and preservation in analytical chemistry, and in particular to a device and method for quantitatively collecting, processing and preserving liquid samples. Background Art

[0002] Sample collection, often referred to as sampling, is a method of taking samples. Sampling of liquids is the first step in many studies and analyses, such as blood testing, urine testing, water quality testing, chemical laboratory analysis, radioactivity testing, and analysis of liquid components.

[0003] Currently, most liquid sample sampling requires direct manual operation and the use of corresponding measuring devices, such as pipettes, pipettes, and liquid samplers. When the number of samples is small and the sampling volume is appropriate, sampling with universal measuring instruments is feasible. However, when the liquid volume is small, such as sampling volumes as low as microliters or even nanoliters; when the number of samples is large and the specifications are diverse, such as when a single sample requires multiple samples of different volumes, manual sampling with universal measuring instruments will result in obvious measurement inaccuracies and low efficiency.

[0004] Generally speaking, the sampling site and the sample analysis site are located in different locations. For example, blood tests conducted in community clinics often only complete simple processing processes such as quantitative blood sample collection and anticoagulation in the clinic. The blood samples are then transported to the central laboratory for analysis through cold chain. In athlete doping tests, the quantitative collection and fixation of blood samples need to be completed in non-professional places such as the stadium. The collected blood samples not only need to be able to withstand long-distance transportation, but also need to be able to be stored for a long time. From the above examples, we can see that conventional liquid sampling not only includes a single liquid quantitative measurement step, but also includes steps such as liquid sample processing, storage and transportation. It is a systematic project.

[0005] To address these needs for liquid sampling, the present invention proposes a simple and effective integrated liquid sampling solution based on microfluidic technology. This solution utilizes a microfluidic chip for quantitative liquid sample collection and a porous material chip for liquid sample processing and storage. This solution can meet the needs for quantitative liquid sample collection, processing, and storage in non-professional or field environments. Summary of the Invention

[0006] The present invention provides a device and method for quantitative collection, processing and preservation of liquid samples. The device can realize the quantitative collection, processing and preservation of micro-liquid samples from nanoliter to milliliter levels.

[0007] The present invention adopts a microchannel network to quantitatively measure the sample. The present invention can produce multiple samples with accurate volumes in a single collection process, solving the problem of difficulty in quantitatively measuring samples due to the small sample volume in conventional micro-sampling processes. By adopting a porous material chip with a sample processing function to preserve the sample, the present invention solves the problem of cumbersome sample processing and inconvenient preservation when sampling under non-professional conditions or in the field. The liquid quantitative collection, processing and preservation device described in the present invention has a simple structure, is easy to operate, and the samples produced are easy to preserve and transport. Therefore, it is very suitable for sample collection under non-professional conditions or in the field, and has good application prospects in the fields of athlete doping detection, home urine sample collection, and environmental sewage sampling and preservation.

[0008] A device for quantitatively collecting, processing, and storing liquid samples, comprising: a quantitative measuring chip and a porous material chip connected to the quantitative measuring chip via a structural member; the quantitative measuring chip and the porous material chip can have two working states: unfolded and folded;

[0009] The quantitative measuring chip is provided with a microchannel network having an inlet and multiple outlets; the porous material chip is provided with multiple processing and storage units which are docked with the multiple outlets when in a folded working state.

[0010] Using the above technical solution, the quantitative measuring chip and the porous material chip are in an unfolded state during sampling, avoiding the influence of the porous material chip on sampling and ensuring the quantitative sampling of the quantitative measuring chip; after sampling is completed, the quantitative measuring chip and the porous material chip are folded in half to complete the processing of the liquid sample and finally achieve the preservation of the sample.

[0011] Preferably, the microchannel network can be provided as a single one or as a plurality of independent ones to enable quantitative sampling and storage of multiple or different samples. Each independent microchannel network has one inlet and two or more outlets. Each inlet and outlet has a corresponding microchannel. The microchannel network can be either closed or open to meet different occasions and detection needs.

[0012] The porous material chip has sample processing and storage functions, and has one or more independent processing and storage units on the porous material chip. Each group of processing and storage units can process and store one or more samples.

[0013] The structural member of the present invention primarily provides flexible fixation between the quantitative sampling chip and the porous material chip. This prevents the porous material chip from being lost during sampling and facilitates quick folding of the porous material chip into place. Preferably, the structural member is a hinge.

[0014] The hinge is a metal part or a plastic part. As a further preference, the hinge is a plastic part, which further facilitates processing and avoids contamination of the sample.

[0015] Preferably, the structural part includes an upper plate for fixing the porous material chip, a lower plate for fixing the quantitative measuring chip, and a hinged part that hinges the upper plate and the lower plate; during actual processing, the upper plate, the lower plate and the hinged part are a plastic part of an integrated structure, which can be processed through a one-time molding process.

[0016] Preferably, the quantitative measuring chip or the porous material chip is detachably connected to the structural member, which further facilitates the processing and manufacture of the quantitative collection, processing and storage device of the present invention.

[0017] Preferably, the microchannel network comprises multiple independent microchannels, each having the outlet at one end and a single inlet at the other end. Using a single inlet allows for the sampling of multiple quantitative samples of the same sample; using multiple outlets allows for the separate processing and storage of multiple quantitative samples.

[0018] Preferably, the interior of the microchannel network has an affinity for the sample, and upon contacting the inlet, the sample liquid will fill the microchannel network under capillary action. Preferably, the interior of the microchannel network has been treated to be hydrophilic, and the inner wall is hydrophilic. Specifically, as a preferred embodiment, the inner wall of the microchannel has been treated to be hydrophilic.

[0019] The inlet surface of the quantitative measurement chip is partially hydrophobicized, forming an automatic liquid sample isolation structure, thereby achieving automatic liquid isolation. Specifically, one end surface of the microchannel at the inlet is hydrophobicized. After the sample enters the microchannel, the affinity of the channel inner wall and the hydrophobic effect of the microchannel inlet end surface further prevent the sample from flowing across multiple microchannels, achieving quantitative and automatic isolation.

[0020] In the present invention, the volumes of the microchannels corresponding to different outlets in a single microchannel network can be the same or different. The total volume of a single microchannel network can be 10 nL to 1 mL, preferably 10 μL to 100 μL. The volume of each microchannel can be determined based on actual needs.

[0021] Furthermore, the material of the quantitative measuring chip may be, but is not limited to, plastic, glass, polydimethylsiloxane, etc. Preferably, the material of the quantitative measuring chip may be polymethyl methacrylate.

[0022] The quantitative measurement chip can be a monolithic or split structure. Preferably, the quantitative measurement chip includes a sub-chip having the microchannel network and a sealing sheet that interfaces with the sub-chip to form a sealed microchannel network. With this structure, when open microchannels are required, the sealing sheet can be removed, and the liquid sample can be sampled normally under capillary action.

[0023] Furthermore, the porous material chip can be made of, but not limited to, paper, sintered polyethylene, porous glass, etc. Preferably, the porous material chip is made of filter paper.

[0024] Preferably, the apparatus further includes a foldable cover plate that is detachably fixedly connected to the structural member, the porous material chip being fixed to the foldable cover plate. The foldable cover plate can be folded to protect the porous material chip after quantitative collection and processing of the liquid sample is completed. The foldable cover plate structure can not only achieve positioning and fixation of the porous material chip, but also provide a certain degree of protection for the sample after the operation and processing is completed, facilitating further storage.

[0025] Preferably, a raised interface is provided on one side of the quantitative measuring chip, and the outlets are connected to corresponding structures.

[0026] Preferably, the processing and saving unit includes one or more combinations of the following units:

[0027] a mixing unit for mixing with a liquid sample;

[0028] Filtration units for filtering liquid samples;

[0029] An enrichment unit for enriching a component or multiple components in a liquid sample;

[0030] Drying unit for drying liquid samples.

[0031] For mixing units, reagents or samples to be mixed can be pre-loaded at the corresponding locations. For filtration units, channel structures can be pre-processed to guide the flow of samples, ultimately achieving directional filtration. For enrichment units, additional enrichment methods can be added. For example, for operations requiring antigen enrichment, antibody materials can be added to the corresponding areas of the enrichment unit. For drying units, drying materials can be added to the corresponding areas.

[0032] Furthermore, the processing and storage unit is loaded with materials for sample processing, including but not limited to desiccants, antimicrobial agents, antibodies, etc. Preferably, the processing and storage unit is loaded with desiccants such as anhydrous magnesium sulfate and / or antimicrobial agents such as sodium benzoate and / or antibodies for enrichment.

[0033] Furthermore, the processing and storage unit may have a channel structure to perform functions such as mixing, filtering, enrichment, etc. Preferably, the processing and storage unit may use, but is not limited to, hydrophilic channels obtained by wax spraying or cutting processes.

[0034] The present invention provides a method for quantitatively collecting, processing, and preserving a liquid sample, which is performed using the device described in any of the above technical solutions and comprises the following steps:

[0035] (1) In the expanded state, the inlet of the microchannel network is brought into contact with the liquid sample. Under the action of surface tension, the liquid sample fills the entire microchannel network.

[0036] (2) Folding the quantitative measurement chip and the porous material chip in half, the outlet of the microchannel network is aligned with the corresponding processing and storage unit of the porous material chip;

[0037] (3) Flipping the folded quantitative measuring chip and the porous material chip, with the inlet facing upward and the outlet located at the bottom, so that the liquid sample in the microchannel network contacts the processing and storage unit of the porous material chip under the action of gravity;

[0038] (4) Under the action of the surface tension inside the porous material chip, the sample in the microchannel network is transferred to the porous material chip and automatically diffuses in the hydrophilic channel to achieve corresponding operations (diffusion, mixing, filtration, enrichment, etc.);

[0039] (5) The porous material chip with the sample is stored in a sealed environment.

[0040] For the technical solution with hydrophobic treatment on the inlet end, after the body sample leaves the inlet, under the action of the local hydrophobic surface of the inlet, the residual liquid at the inlet will automatically form partitions between different channels under the action of surface tension.

[0041] Furthermore, in step (4), the sample processing material and the liquid sample can be mixed during the sample transfer process.

[0042] Furthermore, in step (4), the diffusion process of the sample in the hydrophilic channel can realize a filtering function.

[0043] Furthermore, in step (4), with the help of pre-loaded antibodies, the diffusion process of the sample in the hydrophilic channel can achieve the enrichment function of specific antigens.

[0044] Furthermore, in step (4), the moisture in the liquid sample adsorbed by the porous material chip is gradually absorbed by the sample processing material to form a dry sample.

[0045] The advantages of the present invention are:

[0046] The present invention has the ability to quantitatively measure liquid samples from nanoliter to milliliter levels, and can form one or more liquid samples with accurate volumes through one sampling.

[0047] The present invention combines liquid sample collection, processing and preservation. After the liquid sample is collected, post-processing such as antibacterial, drying and enrichment is immediately performed, so that the sample can be stored on the porous material chip for a long time, which greatly facilitates the collection, processing and preservation of liquid samples.

[0048] The present invention has a simple structure, is easy to operate, and can maintain reliable performance even in non-professional conditions or field conditions. At the same time, the present invention is low in cost and has the feasibility and value of being widely promoted and used. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 It is a schematic diagram of the structure of a device for quantitative collection, processing and storage of liquid samples.

[0050] Figure 2 This is a schematic diagram of the specific structure of a quantitative collection, processing and storage device in Example 1.

[0051] Figure 3 This is a distribution diagram of the inlet hydrophobic area in Example 1.

[0052] Figure 4 This is a schematic diagram of the method of using the device in Example 1.

[0053] Figure 5 It is a structural diagram of the processing and storage unit 6 of Example 2. DETAILED DESCRIPTION

[0054] The present invention will be described below with examples.

[0055] like Figure 1 As shown, a device for quantitative collection, processing and storage of liquid samples includes: a quantitative measuring chip 1 with a microchannel network, a porous material chip 5 with sample processing and storage functions, and a structural member 7 for fixing the quantitative measuring chip 1 and the porous material chip 5.

[0056] The quantitative measurement chip 1 has one or more independent microchannel networks 2, each of which includes three microchannels 8, one inlet 3, and two or more outlets 4. In this embodiment, there is one microchannel network and three outlets. Each inlet 3 and outlet 4 has a corresponding microchannel 8. The microchannel network 2 can be either closed or open.

[0057] The porous material chip 5 is provided with one or more independent processing and storage units 6. Each group of processing and storage units 6 can process and store one or more samples.

[0058] The interior of the microchannel network 2 has affinity with the sample, and the sample liquid will fill the microchannel network 2 under capillary force after contacting the inlet 3. Preferably, the interior of the microchannel network 2 is hydrophilic, so that the inner wall of the microchannel 8 is hydrophilic.

[0059] The local surface of the inlet 3 of the quantitative measuring chip 1 is treated with hydrophobicity, so that the liquid automatic isolation function can be realized. Figure 3 Shown is a schematic diagram of the area where the local surface at the inlet 3 has been hydrophobically treated.

[0060] The volumes of the microchannels 8 corresponding to different outlets 4 in a single microchannel network can be the same or different. The total volume of a single microchannel network 2 can be 10 nL to 1 mL, preferably, the total volume of the microchannel network is 10 μL to 100 μL.

[0061] The material of the quantitative measuring chip 1 can be, but is not limited to, plastic, glass, polydimethylsiloxane, etc. Preferably, the material of the quantitative measuring chip 1 can be polymethyl methacrylate.

[0062] The porous material chip 5 can be made of, but is not limited to, paper, sintered polyethylene, porous glass, etc. Preferably, the porous material chip 5 is made of filter paper.

[0063] The processing and storage unit 6 is loaded with materials for sample processing, including but not limited to desiccant, antimicrobial agent, antibody, etc. Preferably, the processing and storage unit 6 is loaded with desiccant such as anhydrous magnesium sulfate and / or antimicrobial agent such as sodium benzoate and / or enrichment antibody.

[0064] The processing and storage unit 6 may have a channel structure to perform functions such as mixing, filtering, and enrichment. Preferably, the processing and storage unit 6 may use, but is not limited to, a hydrophilic channel obtained by wax spraying or cutting.

[0065] like Figure 2 This is a schematic structural diagram of a specific implementation scheme of the device in this embodiment:

[0066] In the figure, the structural part 7 includes an upper plate 701 for fixing the porous material chip 5, a lower plate 702 for fixing the quantitative measuring chip 1, and a hinge part 703 for hingedly connecting the upper plate 701 and the lower plate 702; during actual processing, the upper plate 701, the lower plate 702 and the hinge part 703 are plastic parts of an integrated structure, which can be processed through a one-time molding process.

[0067] The quantitative measurement chip 1 includes a sub-chip with the microchannel network, and a sealing plate that is shaped like the sub-chip and can be docked with the sub-chip to form a sealed microchannel network. The quantitative measurement chip 1 is provided with snap-on lugs 101 on both sides; the lower plate 702 is provided with a snap-on plate 704 that engages with the snap-on lugs 101.

[0068] The porous material chip 5 is fixed to the upper plate 701 by means of a folding cover plate 501, and a card member for engaging the folding cover plate 501 is also provided on the upper plate 701. There can be multiple porous material chips 5, and two are independently arranged in the figure. Two folding cover plates 501 are also provided. The folding cover plate 501 is provided with a card member 503 for engaging the porous material chip 5, which is used to fix the porous material chip 5. At the same time, the folding cover plate 501 is also provided with a folding positioning card plate 502 for fixing it after it is folded. After the sample processing is completed, before storage, the folding cover plate 501 with the porous material chip 5 is folded along its crease to store the sample in the folding cavity, and at the same time, it is maintained in a folded state by the folding positioning card plate 502, and then stored.

[0069] The present invention provides an operating method of a device for quantitatively collecting, processing and storing liquid samples:

[0070] (1) Expand the porous material chip 5 and the quantitative measurement chip 1. Figure 4 In Figure 1, the inlet 3 of a microchannel network 2 is brought into contact with a liquid sample. Under the action of surface tension, the liquid sample will fill the entire microchannel network 2.

[0071] (2) After the liquid sample enters the microchannel 8 and leaves the inlet 3, under the action of the local hydrophobic surface of the inlet 3, the residual liquid in the inlet will automatically form a partition between different channels under the action of surface tension.

[0072] (3) With the cooperation of the structural member 7, align the microchannel network outlet 4 with the processing and storage unit 6 on the porous material chip 5. The structural member 7 can be a hinge member, which directly folds the quantitative measuring chip 1 and the porous material chip 5 in half and fixes them, while ensuring that the microchannel network outlet 4 is aligned with the processing and storage unit 6 on the porous material chip 5. Figure 4 Middle II

[0073] (4) Turn the entrance 3 upward and the exit 4 downward, and fold it. Figure 4 In Figure III, under the action of gravity, the liquid sample in the microchannel network 2 contacts the porous material chip 5.

[0074] (5) Under the action of the surface tension inside the porous material chip 5, the sample in the microchannel network 2 is transferred to the porous material chip 5 and automatically diffuses in the hydrophilic channel to achieve functions such as mixing, filtration, and enrichment.

[0075] (6) Open the porous material chip 5 and the quantitative measurement chip 1. Figure 4 In step IV, the porous material chip 5 is taken out, the folding cover plate is folded into place, and the sample is then stored in a sealed environment.

[0076] In step 5, the mixing of the sample processing material and the liquid sample can be completed during the sample transfer process.

[0077] In step 5, the diffusion process of the sample in the hydrophilic channel can realize the filtering function.

[0078] In step 5, with the help of pre-loaded antibodies, the diffusion process of the sample in the hydrophilic channel can achieve the enrichment function of specific antigens.

[0079] In step 6, the moisture in the liquid sample adsorbed by the porous material chip 5 is gradually absorbed by the sample processing material to form a dry sample.

[0080] The present invention is further described below by taking a specific application as an example:

[0081] Example 1

[0082] The present invention is applied to the on-site blood sample collection and storage for doping detection of athletes, such as Figures 2-4 shown.

[0083] In Example 1, the quantitative measuring chip 1 is made of polymethyl methacrylate and has a microchannel network 2. The microchannel network has an inlet 3 and three outlets 4, and each outlet 4 has a corresponding microchannel 8 with a volume of 20 μL.

[0084] In Example 1, the inlet 3 of the microchannel network 2 has some hydrophobic treatment surfaces 12. Figure 3 As shown, these hydrophobic treated surfaces 12 enable the liquid in the microchannels 8 to be automatically separated at the inlet 3 when the liquid sample leaves the inlet 3 .

[0085] In Example 1, the microchannel 8 is closed, and the inner surfaces of the microchannel 8 and the outlet 4 are both subjected to surface hydrophilic treatment.

[0086] In Example 1, there are two porous material chips 5, each of which contains a set of independent processing and storage units 6. The two sets of processing and storage units can process one and two samples respectively. The porous material chip 5 is made of filter paper.

[0087] In Example 1, anhydrous magnesium sulfate is loaded as a sample processing material on the processing and storage unit 6. Anhydrous magnesium sulfate can quickly dry the collected blood sample.

[0088] In Example 1, the structural member 7 is made of polypropylene and has a hinge structure, which facilitates the alignment of the quantitative measuring chip 1 with the processing and storage unit 6 on the porous material chip 5 .

[0089] In Example 1, the device is used as follows:

[0090] I. The inlet 3 of the microchannel network 2 is brought into contact with fingertip blood or upper arm blood. Under the action of surface tension, the blood sample quickly enters the multiple microchannels 8 and fills the entire microchannel network 2. After the blood sample leaves the inlet 3, the residual blood at the inlet is automatically separated from the different channels by the surface tension due to the local hydrophobic surface of the inlet 3.

[0091] II. With the cooperation of the structural member 7 , align the microchannel network outlet 4 with the processing and storage unit 6 on the porous material chip 5 .

[0092] III. With inlet 3 facing upward and outlet 4 facing downward, gravity forces the blood sample in microchannel network 2 into contact with porous material chip 5. Surface tension within porous material chip 5 transfers the blood sample from microchannel network 2 to the porous material chip 5, where it mixes with the sample processing material, anhydrous magnesium sulfate, rapidly drying the blood sample.

[0093] IV. Open the structural member 7, and the dried blood sample can be sealed and stored for a long time.

[0094] Example 2

[0095] The present invention is applied to the collection of urine samples, such as Figure 5 shown.

[0096] In Example 2, the porous material chip 5 is made of cellulose filter paper and is wax-sprayed to form a processing and preservation unit 6 with hydrophilic channels. Specific antibodies 11 are embedded in the processing and preservation unit 6 as sample processing materials. When a urine sample diffuses from the microchannel outlet 4 at a corresponding location 10 on the porous material chip 5 to other locations in the hydrophilic channels of the processing and preservation unit 6, trace amounts of antigens in the urine sample can be captured by the specific antibodies 11, thereby enriching the trace antigens in the urine.

[0097] In Example 2, the processing and preservation unit 6 is also loaded with anhydrous magnesium sulfate as a sample processing material to facilitate rapid drying and long-term preservation of the sample.

[0098] Example 3

[0099] The present invention is applied to collecting environmental water samples for detecting toxins in water blooms.

[0100] In Example 3, the microchannels 8 in the microchannel network 2 are not closed but open, thereby preventing large particles of impurities from clogging the microchannels and causing sampling failure during the collection of environmental water samples.

[0101] In Example 3, the porous material chip 5 is made of sintered polyethylene that has been hydrophilically treated to ensure that the porous material chip can withstand environmental water samples with complex components.

[0102] In Example 3, the sample treatment material includes sodium benzoate and anhydrous magnesium sulfate. Sodium benzoate can quickly inactivate microbial activity in the water sample, preventing sample deterioration during storage, while anhydrous magnesium sulfate can dry the sample for storage.

Claims

1. A device for quantitative collection, processing and storage of liquid samples, characterized in that: include: A quantitative measuring chip and a porous material chip connected to the quantitative measuring chip via a structural member; The quantitative measuring chip and the porous material chip have two working states: unfolded and folded; The quantitative measurement chip is provided with a microchannel network having an inlet and multiple outlets, and an automatic liquid sample isolation structure is provided at the inlet of the microchannel network; the porous material chip is provided with multiple processing and storage units that are connected to the multiple outlets when in a folded working state; The microchannel network includes a plurality of mutually independent microchannels, one end of each microchannel is provided with the outlet, and the other end of the plurality of microchannels is centrally arranged to form the inlet; The inner wall of the microchannel is treated to be hydrophilic; the end face of the microchannel at the entrance is treated to be hydrophobic to form an automatic liquid sample isolation structure; The processing and storage unit includes one or more combinations of the following units: Filtration units for filtering liquid samples; An enrichment unit for enriching a component or multiple components in a liquid sample; a drying unit for drying liquid samples; When using: Fold the quantitative measuring chip and the porous material chip in half, so that the outlet of the microchannel network is aligned with the corresponding processing and storage unit of the porous material chip; Under the action of the surface tension inside the porous material chip, the sample in the microchannel network is transferred to the porous material chip and automatically diffuses in the hydrophilic channel to achieve the corresponding operation; The porous material chip with the sample is stored in a sealed environment.

2. The device for quantitative collection, processing and storage of liquid samples according to claim 1, characterized in that: The structural member is a hinge member.

3. The device for quantitative collection, processing and storage of liquid samples according to claim 1, characterized in that: The quantitative measuring chip or the porous material chip is detachably connected to the structural member.

4. The device for quantitative collection, processing and storage of liquid samples according to claim 1, characterized in that: The quantitative measuring chip comprises a sub-chip provided with the micro-channel network, and a sealing sheet connected to the sub-chip to form a sealed micro-channel network channel.

5. The device for quantitative collection, processing and storage of liquid samples according to claim 1, characterized in that: It also includes a foldable cover plate that is detachably fixedly connected to the structural member, and the porous material chip is fixed on the foldable cover plate. After the quantitative collection and processing of the liquid sample are completed, the foldable cover plate can be folded to protect the porous material chip.

6. The device for quantitative collection, processing and storage of liquid samples according to claim 1, characterized in that: A raised interface is provided on one side of the quantitative measuring chip, and the outlets are connected to corresponding structures.

7. A method for quantitatively collecting, processing, and preserving a liquid sample, using the device according to any one of claims 1 to 6, comprising the following steps: (1) In the expanded state, the inlet of the microchannel network is brought into contact with the liquid sample. Under the action of surface tension, the liquid sample fills the entire microchannel network. (2) Fold the quantitative measurement chip and the porous material chip in half, so that the outlet of the microchannel network is aligned with the corresponding processing and storage unit of the porous material chip; (3) Flipping the folded quantitative measuring chip and the porous material chip, with the inlet facing upward and the outlet located at the bottom, the liquid sample in the microchannel network contacts the processing and storage unit of the porous material chip; (4) Under the action of the surface tension inside the porous material chip, the sample in the microchannel network is transferred to the porous material chip and automatically diffuses in the hydrophilic channel to achieve the corresponding operation; (5) The porous material chip with the sample is stored in a sealed environment.

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