Microfluidic devices and methods for sampling and analyzing cells using optical forces and raman spectroscopy

By combining hydrodynamics, photodynamics, electrodynamics, and Raman spectroscopy into a microfluidic device, the flow rate control and mixing problems in biological cell analysis in existing technologies have been solved, enabling automated sampling and label-free biochemical data analysis, and supporting process monitoring in cell therapy and gene therapy.

CN114641450BActive Publication Date: 2026-04-24LUMACYTE LLC
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LUMACYTE LLC
Filing Date
2020-09-09
Publication Date
2026-04-24

AI Technical Summary

Technical Problem

Existing technologies struggle to achieve accurate nanoliter flow rate control, adequate sample mixing, and temperature control in biological cell analysis, making them unsuitable for laser force cytology, Raman spectroscopy, or other photodynamic and hydrodynamic measurements, and failing to meet the needs of automated sampling, mixing, and temperature maintenance for biological cells.

Method used

A microfluidic device combining hydrodynamics, optical force, electrodynamics, and Raman spectroscopy is used to separate, analyze, and sort cells via fiber optic probes and microfluidic channels, and surface-enhanced Raman signals are used to enhance the measurement results.

Benefits of technology

It enables automated sampling, mixing, and temperature control of biological cells, allows for accurate fluid flow control and particle sorting, provides label-free biochemical data analysis, and supports process monitoring and quality control for cell therapy and gene therapy.

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Abstract

Provided herein are devices and methods for automated analysis of one or more samples in a single- or multi-well plate or vessel, wherein the automated analysis process includes flow and fluidic, electrokinetic, and optical forces for analysis and sorting of samples, wherein the samples include liquids or particles in microfluidic channels, and wherein the devices include component assemblies capable of processing the samples for analytical evaluation based on the fluids and / or particles. Microfluidic structures (channels, "T" junctions, "Y" junctions, branched "Y" junctions, wells, and weirs) are described for facilitating sample interactions and observation, sample analysis, sorting, or partitioning. Detection can be accomplished using spectroscopic methods, including but not limited to Raman spectroscopy of single cells and bulk cell samples (cell populations; a few individuals to hundreds or thousands of cells).
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Citation Information

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