A low-temperature storage device capable of automatically storing and taking out samples by magnetic attraction

Abstract

The application discloses a kind of low-temperature storage devices of automatic sample storage and access of magnetic attraction, including liquid nitrogen tank, rotating positioning assembly, storage assembly, sample access assembly and control assembly;Liquid nitrogen tank is provided with access, rotating positioning assembly is composed of outer rotating shaft, middle rotating shaft and inner rotating shaft, storage assembly is composed of upper storage disc, middle storage disc and lower storage disc, and the three rotating shafts of rotating positioning assembly are connected with the three storage discs of storage assembly respectively;Three storage discs are sequentially spaced a predetermined distance from top to bottom, and different storage discs can rotate independently, facilitating the access of cryopreservation tube cabin on each storage disc.The low-temperature storage device of the application solves the problem of taking out other cryopreservation tubes when extracting a single cryopreservation tube from the liquid nitrogen tank, avoids the problem of repeated freezing and thawing of biological samples, and ensures the low-temperature safety of biological samples.

Description

Technical Field

[0001] This application relates to the field of biological sample storage, and more specifically, to a magnetically assisted, automated sample storage device for cryogenic storage. Background Technology

[0002] When cryopreserving biological samples using liquid nitrogen, a combination of cryovials, cryovials, and baskets is typically used. First, the acquired biological sample is placed in a cryovial, then the cryovial is placed in a cryovial, and finally the cryovial is placed in a basket and immersed in liquid nitrogen for cryopreservation. When storing or retrieving a single cryovial, the entire basket must first be removed from the liquid nitrogen environment. Then, the corresponding cryovial must be located, and finally, the cryovial is placed in or removed from the cryovial. This practice of removing other samples from the basket when storing or retrieving a single cryovial exposes the entire basket to room temperature, which can damage the frozen sample. This is especially true when such operations are frequent, as repeated freezing and thawing of unrelated biological samples can cause significant damage. Summary of the Invention

[0003] This application provides a magnetically assisted automatic sample storage device for cryopreservation, which enables independent automatic storage and retrieval of a single cryopreservation tube, solving the problem that biological samples in multiple cryopreservation tubes in the entire basket are exposed to room temperature when storing (or retrieving) a single cryopreservation tube.

[0004] This invention includes a liquid nitrogen tank, a rotary positioning assembly, a storage assembly, a sample storage and retrieval assembly, and a control assembly.

[0005] The liquid nitrogen tank includes a tank body, an upper end cover, an access port cover, and an outer cover. The upper end cover and the outer cover are provided at the upper end of the tank body. The upper end cover has a sample access port and an access port cover at an off-center position. An upper bearing seat is provided at the center of the upper end cover. A lower bearing seat is provided at the center of the bottom of the liquid nitrogen tank. The outer cover is dome-shaped and covers the upper end cover. The tank body is provided with a handle and a fixing groove.

[0006] The rotary positioning assembly includes an outer rotary shaft, a middle rotary shaft, an inner rotary shaft, an outer rotary shaft motor, a middle rotary shaft motor, and an inner rotary shaft motor. The outer rotary shaft and the middle rotary shaft are hollow shafts, while the inner rotary shaft is a solid shaft. The outer diameters of the outer rotary shaft, the middle rotary shaft, and the inner rotary shaft decrease sequentially, increase sequentially by a predetermined length, and are concentrically fitted together at the center of the liquid nitrogen tank. The upper end of each shaft is installed in the upper bearing seat, and the lower end is installed in the lower bearing seat. Bearings are installed between the interlocking rotary shafts. The outer rotary shaft motor, the middle rotary shaft motor, and the inner rotary shaft motor are all mounted on the upper end cover, respectively controlling the rotation of the outer rotary shaft, the middle rotary shaft, and the inner rotary shaft.

[0007] The storage assembly includes an upper storage tray, a middle storage tray, a lower storage tray, and a cryopreservation chamber. The upper, middle, and lower storage trays are installed at predetermined intervals from top to bottom within the liquid nitrogen tank, respectively, at the lower ends of the outer, middle, and inner rotating shafts. The upper and middle storage trays are provided with access notches and access gaps, respectively. Storage wells are provided on the upper, middle, and lower storage trays. Adsorption magnets are installed at the bottom of the storage wells, and small holes are provided around the storage wells. The cryopreservation chamber includes a chamber body and a cover. The chamber body and the cover are connected by threads. An upper iron plate is provided at the upper end of the cover, and a lower iron plate is provided at the lower end of the chamber body.

[0008] The sample storage and retrieval assembly includes a rotating platform, a cross slide, and a gripping mechanism. The rotating platform is fixedly mounted on the fixed slot, the cross slide is mounted on the rotating platform, and the gripping mechanism is mounted on the cross slide. The gripping mechanism includes a long-handled sleeve, a storage magnet, and an extraction magnet. The storage magnet is installed at the lower end of the long-handled sleeve during storage, and the extraction magnet is installed at the lower end of the long-handled sleeve during extraction. The magnetic force of the extraction magnet is greater than that of the adsorption magnet, and the magnetic force of the adsorption magnet is greater than that of the storage magnet.

[0009] The control components include a PLC controller and a display screen;

[0010] The upper storage disk, middle storage disk and lower storage disk are provided with regularly arranged storage holes, and the storage well is located below the storage holes. The opening of the storage well is perpendicular to the storage hole. The storage well is made of aluminum alloy.

[0011] The rotating platform is driven by its own rotating motor;

[0012] The cross slide includes a horizontal linear module and a vertical linear module. The horizontal linear module is a ball screw type linear module, which is driven by its own horizontal motor to control the horizontal movement of its own horizontal slider. The vertical linear module is a ball screw type linear module, which is driven by its own vertical motor to control the vertical movement of its own vertical slider.

[0013] The lower end of the long-handled sleeve is provided with an internal thread, the storage magnet and the extraction magnet are cylindrical magnets, and the upper end of the storage magnet and the extraction magnet are provided with an external thread that matches the internal thread of the long-handled sleeve.

[0014] The adsorption magnet, the storage magnet, and the extraction magnet are all neodymium magnets;

[0015] The input terminals of the outer rotary axis motor, the middle rotary axis motor, the inner rotary axis motor, the rotary platform motor, the horizontal motor, and the vertical motor are connected to the output terminal of the PLC controller, and their operation is controlled by the PLC controller.

[0016] The positive effects of this invention are as follows:

[0017] In this embodiment, a liquid nitrogen tank is used to maintain the low temperature inside the tank. The liquid nitrogen tank has an access port. Three storage trays are disposed inside the liquid nitrogen tank, arranged sequentially from top to bottom at predetermined intervals. All storage trays except the bottom one have access notches for a sample access component to pass through. The storage trays store cryopreservation chambers, which contain cryopreservation tubes. A rotary positioning assembly includes three rotating shafts, each controlled independently by a rotary motor. The three rotating shafts correspond one-to-one with and are fixedly connected to the three storage trays, driving each tray to rotate. A sample access component is used to grasp a cryopreservation chamber from any storage tray through the access port. The cryopreservation tubes in the chamber are used to store samples.

[0018] With this storage device, different storage trays can be rotated, and cryopreservation tube chambers of each layer can be flexibly accessed through the notches on the storage trays. The cryogenic storage device in this embodiment solves the problem caused by the exposure of all cryopreservation tubes in the basket when extracting cryopreservation tubes from the liquid nitrogen tank, avoids repeated freeze-thaw cycles of biological samples, and ensures the low-temperature safety of biological samples. Attached Figure Description

[0019] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. In the drawings:

[0020] Figure 1 This is a schematic diagram of the appearance of a magnetic automatic sample storage device according to an embodiment of this application;

[0021] Figure 2 This is a schematic diagram of the structure of a magnetic automatic sample storage device according to an embodiment of this application;

[0022] Figure 3 This is a partial structural diagram of the gripping mechanism according to an embodiment of this application;

[0023] Figure 4 This is a schematic diagram of a partial internal structure of the liquid nitrogen tank according to an embodiment of this application;

[0024] Figure 5 This is a schematic diagram of a storage disk structure according to an embodiment of this application;

[0025] Figure 6 This is a schematic diagram of a storage well structure according to an embodiment of this application;

[0026] Figure 7 This is a schematic diagram of the cryogenic storage chamber and storage well structure according to an embodiment of this application;

[0027] Figure 8 This is a flowchart of the sample storage process according to an embodiment of this application;

[0028] Figure 9 This is a flowchart of the sample extraction process according to an embodiment of this application.

[0029] Explanation of icon numbers:

[0030] Liquid nitrogen tank 1: tank body 101; handle 1011; fixing groove 1012; upper end cover 102; access port cover 103; sample access port 1031; outer cover 104; upper bearing seat 1051; lower bearing seat 1052;

[0031] Rotary positioning assembly 2: outer rotary shaft 201-1; middle rotary shaft 201-2; inner rotary shaft 201-3; outer rotary shaft motor 202-1; middle rotary shaft motor 202-2; inner rotary shaft motor 202-3;

[0032] Storage Component 3: Upper storage disk 301; Middle storage disk 302; Lower storage disk 303; Cryopreservation tube compartment 3001; Storage well 3002; Access notch 301-1; Access notch 302-1; Body 3001-1; Cover 3001-2; Upper iron plate 3001-3; Lower iron plate 3001-4; Adsorption magnet 3002-1; Small hole 3002-2;

[0033] Sample storage and retrieval component 4: Rotating platform 401; Cross slide 402; Gripping mechanism 403; Horizontal linear module 402-1; Horizontal slider 402-1-1; Horizontal motor 402-1-2; Vertical linear module 402-2; Vertical slider 402-2-1; Vertical motor 402-2-2; Long-handled sleeve 403-1; Storage magnet 403-2; Retrieval magnet 403-3;

[0034] Control component 5. Detailed Implementation

[0035] It should be noted that the flowchart in the attached figure shows the logical order of sample storage and retrieval, but in some cases, the steps shown or described may be performed in a different order than that shown here.

[0036] In this embodiment, a cryogenic storage device is provided, comprising: a liquid nitrogen tank for maintaining a low temperature by using liquid nitrogen, the liquid nitrogen tank having an access port; three storage trays disposed within the liquid nitrogen tank, the three storage trays being arranged sequentially from top to bottom at predetermined intervals within the liquid nitrogen tank, the other three storage trays (except the bottommost storage tray) having access notches for allowing a sample access component to pass through the storage tray, the storage trays for storing cryopreservation chambers, the cryopreservation chambers storing cryopreservation tubes; a rotation positioning assembly comprising three rotating shafts, the three rotating shafts being independently controlled by three rotating motors, the three rotating shafts corresponding one-to-one with and fixedly connected to the three storage trays, for driving the three storage trays to rotate respectively; and a sample access component for grasping a cryopreservation chamber on any storage tray through the access port, wherein the cryopreservation tube in the cryopreservation chamber is used to store samples.

[0037] With this storage device, different storage trays can be rotated, and cryopreservation tube chambers of each layer can be flexibly accessed through the notches on the storage trays. The cryogenic storage device in this embodiment solves the problem caused by the exposure of the entire basket sample when extracting cryopreservation tubes from the liquid nitrogen tank, and reduces damage to other samples. Example

[0038] like Figure 1-9 As shown, the present invention provides a magnetic suction type automatic sample storage and retrieval low temperature storage device, which mainly includes a liquid nitrogen tank 1, a rotation positioning component 2, a storage component 3, a sample storage and retrieval component 4, and a control component 5.

[0039] The liquid nitrogen tank 1 includes a tank body 101, an upper end cover 102, an access port cover 103, an outer cover 104, an upper bearing seat 1051, and a lower bearing seat 1052. The upper end cover 102 and the outer cover 104 are provided at the upper end of the tank body 101. The upper end cover 102 is provided with a sample access port 1031 and an access port cover 103 at an off-center position. The upper bearing seat 1051 is provided at the center of the upper end cover 102. The lower bearing seat 1052 is provided at the center of the bottom of the liquid nitrogen tank 1. The outer cover 104 is dome-shaped and covers the upper end cover 102. Two symmetrical handles 1011 and a fixing groove 1012 are installed on the tank body 101.

[0040] The rotary positioning assembly 2 includes an outer rotary shaft 201-1, a middle rotary shaft 201-2, an inner rotary shaft 201-3, an outer rotary shaft motor 202-1, a middle rotary shaft motor 202-2, and an inner rotary shaft motor 202-3. The outer rotary shaft 201-1 and the middle rotary shaft 201-2 are hollow shafts, while the inner rotary shaft 201-3 is a solid shaft. The outer diameters of the outer rotary shaft 201-1, the middle rotary shaft 201-2, and the inner rotary shaft 201-3 decrease sequentially, their predetermined lengths increase sequentially, and they are concentrically fitted and installed at the center of the liquid nitrogen tank 1. The outer rotary shaft 201-1, the middle rotary shaft 201-2, and the inner rotary shaft 201-3 form a rotary shaft assembly. Its upper end is installed in the upper bearing seat 1051, and its lower end is installed in the lower bearing seat 1052. Bearings are installed between the interlocking rotating shafts. The outer rotating shaft motor 202-1, the middle rotating shaft motor 202-2 and the inner rotating shaft motor 202-3 are all installed on the upper end cover 102, which respectively control the rotation of the outer rotating shaft 201-1, the middle rotating shaft 201-2 and the inner rotating shaft 201-3. The outer rotating shaft motor 202-1, the middle rotating shaft motor 202-2 and the inner rotating shaft motor 202-3 are respectively connected to the outer rotating shaft 201-1, the middle rotating shaft 201-2 and the inner rotating shaft 201-3 through a set of helical gear reduction mechanisms.

[0041] Storage component 3 includes an upper storage disk 301, a middle storage disk 302, a lower storage disk 303, and a cryopreservation chamber 3001. The upper storage disk 301, middle storage disk 302, and lower storage disk 303 are sequentially spaced at a predetermined distance from top to bottom within the liquid nitrogen tank 1, and are respectively installed at the lower ends of the outer rotating shaft 201-1, the middle rotating shaft 201-2, and the inner rotating shaft 201-3. The upper storage disk 301 and the middle storage disk 302 are respectively provided with access notches 301-1 and 302-1 for allowing the sample access component to pass through the corresponding storage disk. Storage wells 3002 are provided on the upper storage disk 301, the middle storage disk 302, and the lower storage disk 303, and adsorption magnets are installed at the bottom of the storage wells 3002. Iron 3002-1, the adsorption magnet 3002-1 is a neodymium magnet, the storage well 3002 is provided with small holes 3002-2 around its perimeter, the cryopreservation chamber 3001 includes a chamber body 3001-1 and a cover 3001-2, the chamber body 3001-1 and the cover 3001-2 are connected by threads, the upper end of the cover 3001-2 is provided with an upper iron plate 3001-3, and the lower end of the chamber body 3001-1 is provided with a lower iron plate 3001-4; the upper storage plate 301, the middle storage plate 302 and the lower storage plate 303 are provided with regularly arranged storage holes, the storage well 3002 is located below the storage holes, the opening of the storage well 3002 is perpendicular to the storage hole, and the storage well 3002 is made of aluminum alloy;

[0042] Sample storage and retrieval assembly 4 includes a rotating platform 401, a cross slide 402, and a gripping mechanism 403. The rotating platform 401 is mounted on a fixing groove 1012 on the side of the liquid nitrogen tank 1. The cross slide 402 is mounted on the rotating platform 401. The gripping mechanism 403 is mounted on the cross slide 402. The gripping mechanism 403 includes a long-handled sleeve 403-1, a storage magnet 403-2, and a retrieval magnet 403-3. The storage magnet 403-2 is mounted on the long-handled sleeve during storage. At the lower end of the sleeve 403-1, the extraction magnet 403-3 is installed and fixed to the lower end of the long-handled sleeve 403-1 during the extraction process. The magnetic force of the extraction magnet 403-3 is greater than that of the adsorption magnet 3002-1, ensuring that the cryopreservation chamber 3001 can be smoothly detached from the storage well 3002 during sample extraction. The magnetic force of the adsorption magnet 3002-1 is greater than that of the storage magnet 403-2, ensuring that the cryopreservation chamber 3001 can remain in the storage well 3002 during sample storage.

[0043] The rotary platform 401 is driven and controlled by its own installed rotary platform motor 401-1; the cross slide 402 includes a horizontal linear module 402-1 and a vertical linear module 402-2. Both the horizontal linear module 402-1 and the vertical linear module 402-2 are ball screw type linear modules. The horizontal linear module 402-1 is driven and controlled by its own installed horizontal motor 402-1-2 to move its horizontal slider 402-1-1 horizontally. The vertical linear module 402-2... The vertical slider 402-2-1 moves up and down, driven by its own vertical motor 402-2-2; the lower end of the long-handled sleeve 403-1 has an internal thread; the storage magnet 403-2 and the extraction magnet 403-3 are cylindrical magnets, and the upper ends of the storage magnet 403-2 and the extraction magnet 403-3 have external threads that match the internal thread of the long-handled sleeve 403-1; both the storage magnet 403-2 and the extraction magnet 403-3 are neodymium magnets.

[0044] Control component 5 includes a PLC controller and a display screen. The PLC controller is a Siemens S7-200CN. The input terminals of the outer rotary axis motor 202-1, the middle rotary axis motor 202-2, the inner rotary axis motor 202-3, the rotary platform motor 401-1, the horizontal motor 402-1-2, and the vertical motor 402-2-2 are connected to the output terminals of the PLC controller, and the PLC controller controls the operation of each motor.

[0045] The sample storage procedure in this embodiment is as follows:

[0046] First, the location of the storage well 3002 where the cryopreservation tube is to be stored is determined and the storage settings are configured. Then, the outer cover 104 and the access cover 103 are opened. Next, the PLC controller controls the outer rotating shaft motor 202-1, the middle rotating shaft motor 202-2, and the inner rotating shaft motor 202-3 to work. The outer rotating shaft motor 202-1, the middle rotating shaft motor 202-2, and the inner rotating shaft motor 202-3 drive the outer rotating shaft 201-1, the middle rotating shaft 201-2, and the inner rotating shaft 201-3 to rotate, thereby driving the upper storage disk 301, the middle storage disk 302, and the lower storage disk 303 to rotate, so that the target storage well 3002 where the cryopreservation tube is to be stored is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1. The above positioning example (method) is as follows: If the target storage well 3002 is located in a certain position of the lower storage disk 303, the middle rotating shaft motor 202-2 drives the middle rotating shaft 201-2 and the middle storage disk 302 to rotate, so that the access notch 302-1 of the middle storage disk 302 is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1; the outer rotating shaft motor 202-1 drives the outer rotating shaft 201-1 and the upper storage disk 301 to rotate, so that the access notch 301-1 of the upper storage disk 301 is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1; the inner rotating shaft motor 202-3 drives the inner rotating shaft 201-3 and the lower storage disk 303 to rotate to the target position, that is, the target storage well 3002 is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1; similarly, if the target storage well 3002 is located in a certain position of the lower storage disk 303, the middle rotating shaft motor 202-2 drives the middle rotating shaft 201-1 and the upper storage disk 301 to rotate, so that ... If the target storage well 3002 is located at a certain position of the middle storage disk 302, then it is only necessary to control the outer rotation shaft motor 202-1 to drive the outer rotation shaft 201-1 and the upper storage disk 301 to rotate, so that the access notch 301-1 of the upper storage disk 301 is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1; the middle rotation shaft motor 202-2 drives the middle rotation shaft 201-2 and the middle storage disk 302 to rotate to the target position, that is, the target storage well 3002 is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1; if the target storage well 3002 is located at a certain position of the upper storage disk 301, then it is only necessary to control the outer rotation shaft motor 202-1 to drive the outer rotation shaft 201-1 and the upper storage disk 301 to rotate to the target position, that is, the target storage well 3002 is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1.

[0047] Next, the cryopreservation tubes are placed into the cryopreservation tube compartment 3001, and then the storage magnet 403-2 is installed inside the sleeve 403-1; the upper iron plate 3001-3 of the cryopreservation tube compartment 3001 is attracted and fixed to the storage magnet 403-2; then, the PLC controller controls the rotating platform motor 401-1 to work, the rotating platform motor 401-1 drives the rotating platform 401 to rotate, the rotating platform 401 drives the cross slide 402 and the gripping mechanism 403 to rotate as a whole to above the storage port of the liquid nitrogen tank 1; then, the PLC controller controls the horizontal motor 402-1-2 on the horizontal linear module 402-1 to work, the horizontal motor 402-1-2 drives the horizontal slider 402-1-1 to move horizontally, the horizontal slider 402-1-1 drives the vertical linear module 402-2 and the gripping mechanism 403 to move horizontally as a whole, so that the long-handled sleeve 403-1 and the cryopreservation tube compartment it attracts are vertically aligned with the target storage well 3002; then... The PLC controller controls the vertical motor 402-2-2 to work, which drives the vertical slider 402-2-1 to move vertically downward, thereby driving the gripping mechanism 403 to move downward as a whole. The long-handled sleeve 403-1 inserts the cryopreservation chamber it has attracted into the target storage well 3002. The bottom of the target storage well 3002 attracts the magnet 3002-1 and the lower iron plate 3001-4 on the cryopreservation chamber. The PLC controller controls the vertical motor 402-2-2 to work, which drives the vertical slider 402-2-1 and the gripping mechanism 403 to move upward as a whole. Because the attraction force between the magnet 3002-1 and the lower iron plate 3001-4 is greater than the attraction force between the storage magnet 403-2 and the upper iron plate 3001-3, the storage magnet 403-2 separates from the cryopreservation chamber 3001, and the gripping mechanism 403 moves out of the sample storage port 1031, completing the sample storage.

[0048] The sample extraction process in this embodiment is as follows:

[0049] First, the location of the cryopreservation tube to be extracted in the target storage well 3002 is determined and extraction settings are made. Then, the outer cover 104 and the access cover 103 are opened. Next, the extraction magnet 403-3 is installed inside the long-handled sleeve 403-1. The PLC controller controls the operation of the outer rotating shaft motor 202-1, the middle rotating shaft motor 202-2, and the inner rotating shaft motor 202-3. The outer rotating shaft motor 202-1, the middle rotating shaft motor 202-2, and the inner rotating shaft motor 202-3 respectively drive the outer rotating shaft 201-1, the middle rotating shaft 201-2, and the inner rotating shaft 201-3 to rotate. This causes the upper storage disk 301, middle storage disk 302, and lower storage disk 303 to rotate, aligning the cryopreservation chamber 3001 to be extracted perpendicularly to the sample access port 1031 on the liquid nitrogen tank 1. The above positioning example (method) is as follows: If the cryopreservation chamber 3001 to be extracted is located in a certain position on the lower storage disk 303, the middle rotation axis motor 202-2 drives the middle rotation axis 201-2 and the middle storage disk 302 to rotate, achieving perpendicular alignment between the access notch 302-1 of the middle storage disk 302 and the sample access port 1031 of the liquid nitrogen tank 1; the outer rotation axis motor 202-1 drives the outer rotation axis 201-1 and the upper storage disk 303 to rotate. The storage tray 301 rotates, aligning the access notch 301-1 of the upper storage tray 301 with the sample access port 1031 of the liquid nitrogen tank 1. The inner rotating shaft motor 202-3 drives the inner rotating shaft 201-3 and the lower storage tray 303 to rotate to the target position, aligning the cryopreservation tube chamber 3001 to be extracted with the sample access port 1031 of the liquid nitrogen tank 1. Similarly, if the cryopreservation tube chamber 3001 to be extracted is located in a certain position of the middle storage tray 302, it is only necessary to control the outer rotating shaft motor 202-1 to drive the outer rotating shaft 201-1 and the upper storage tray 301 to rotate, thus aligning the access notch of the upper storage tray 301. 301-1 is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1; the central rotating shaft motor 202-2 drives the central rotating shaft 201-2 and the central storage disk 302 to rotate to the target position, so that the cryopreservation chamber 3001 to be extracted is perpendicularly aligned with the sample access port 1031 of the liquid nitrogen tank 1; if the cryopreservation chamber 3001 to be extracted is located in a certain position of the upper storage disk 301, then it is only necessary to control the outer rotating shaft motor 202-1 to drive the outer rotating shaft 201-1 and the upper storage disk 301 to rotate to the target position, so as to achieve the perpendicular alignment of the cryopreservation chamber 3001 to be extracted with the sample access port 1031 of the liquid nitrogen tank 1.

[0050] Next, the PLC controller controls the rotary platform motor 401-1 to operate, which drives the rotary platform 401 to rotate, thereby causing the cross slide 402 and the gripping mechanism 403 to rotate as a whole above the sample storage port 1031 of the liquid nitrogen tank 1. Then, the PLC controller controls the horizontal motor 402-1-2 on the horizontal linear module 402-1 to operate, which drives the horizontal slider 402-1-1 to move horizontally, thereby driving the vertical linear module 402-2 and the gripping mechanism 403 to move horizontally as a whole, so as to achieve vertical alignment between the long-handled sleeve 403-1 and the cryopreservation tube chamber 3001 to be extracted. Then, the PLC controller controls the vertical motor 402-2-2 to operate, which drives the vertical slide 402-2-2 to move horizontally. The slider 402-2-1 moves vertically downward, thereby driving the gripping mechanism 403 to move downward as a whole until the extraction magnet 403-3 is attracted to the upper iron plate 3001-3. The PLC controller controls the vertical motor 402-2-2 to work, and the vertical motor 402-2-2 drives the vertical slider 402-2-1 and the gripping mechanism 403 to move upward as a whole. Because the attraction force between the extraction magnet 403-3 and the upper iron plate 3001-3 is greater than the attraction force between the adsorption magnet 3002-1 and the lower iron plate 3001-4, the extraction magnet 403-3 adsorbs the cryopreservation tube compartment to be extracted, detaches it from the storage well 3002, and moves upward out of the sample storage port 1031. The cryopreservation tube compartment 3001 is removed, and then the cryopreservation tube is opened and taken out, completing the sample extraction.

[0051] The above are merely embodiments of this application and are not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. A magnetic suction-type automatic sample storage device, characterized in that... It includes a liquid nitrogen tank (1), a rotation positioning assembly (2), a storage assembly (3), a sample storage and retrieval assembly (4), and a control assembly (5). The liquid nitrogen tank (1) includes a tank body (101), an upper end cover (102), an access port cover (103), and an outer cover (104). The upper end cover (102) and the outer cover (104) are provided at the upper end of the tank body (101). The upper end cover (102) is provided with a sample access port (1031) and an access port cover (103) at an off-center position. An upper bearing seat (1051) is provided at the center of the upper end cover (102). A lower bearing seat (1052) is provided at the center of the bottom of the liquid nitrogen tank (1). The outer cover (104) is dome-shaped and covers the upper end cover (102). The tank body (101) is provided with a handle (1011) and a fixing groove (1012). The rotary positioning assembly (2) includes an outer rotary shaft (201-1), a middle rotary shaft (201-2), an inner rotary shaft (201-3), an outer rotary shaft motor (202-1), a middle rotary shaft motor (202-2), and an inner rotary shaft motor (202-3). The outer rotary shaft (201-1) and the middle rotary shaft (201-2) are hollow shafts, and the inner rotary shaft (201-3) is a solid shaft. The outer diameters of the outer rotary shaft (201-1), the middle rotary shaft (201-2), and the inner rotary shaft (201-3) decrease sequentially and increase sequentially. The shafts are installed in a predetermined length and concentrically connected at the center of the liquid nitrogen tank (1). The upper end of the shaft is installed in the upper bearing seat (1051), and the lower end is installed in the lower bearing seat (1052). Bearings are installed between the mutually connected rotating shafts. The outer rotating shaft motor (202-1), the middle rotating shaft motor (202-2), and the inner rotating shaft motor (202-3) are all installed on the upper end cover (102) to control the rotation of the outer rotating shaft (201-1), the middle rotating shaft (201-2), and the inner rotating shaft (201-3), respectively. The storage component (3) includes an upper storage disk (301), a middle storage disk (302), a lower storage disk (303), and a cryopreservation chamber (3001). The upper storage disk (301), the middle storage disk (302), and the lower storage disk (303) are installed at predetermined intervals from top to bottom in the liquid nitrogen tank (1) at the lower ends of the outer rotating shaft (201-1), the middle rotating shaft (201-2), and the inner rotating shaft (201-3), respectively. The upper storage disk (301) and the middle storage disk (302) are respectively provided with access notches (301-1) and (302-1). The upper storage disk (301) Storage wells (3002) are provided on the middle storage disk (302) and the lower storage disk (303). An adsorption magnet (3002-1) is installed at the bottom of the storage well (3002). Small holes (3002-2) are provided around the storage well (3002). The cryopreservation tube compartment (3001) includes a compartment body (3001-1) and a compartment cover (3001-2). The compartment body (3001-1) and the compartment cover (3001-2) are connected by threads. An upper iron plate (3001-3) is provided at the upper end of the compartment cover (3001-2), and a lower iron plate (3001-4) is provided at the lower end of the compartment body (3001-1). The sample storage and retrieval component (4) includes a rotating platform (401), a cross slide (402), and a gripping mechanism (403). The rotating platform (401) is fixedly mounted on the fixing groove (1012). The cross slide (402) is mounted on the rotating platform (401). The gripping mechanism (403) is mounted on the cross slide (402). The gripping mechanism (403) includes a long-handled sleeve (403-1), a storage magnet (403-2), and a retrieval magnet (403-3). 2) During storage, the extraction magnet (403-3) is installed at the lower end of the long-handled sleeve (403-1). During extraction, the extraction magnet (403-3) is installed at the lower end of the long-handled sleeve (403-1). The magnetic force of the extraction magnet (403-3) is greater than that of the adsorption magnet (3002-1), and the magnetic force of the adsorption magnet (3002-1) is greater than that of the storage magnet (403-2). The adsorption magnet (3002-1), the storage magnet (403-2), and the extraction magnet (403-3) are all neodymium magnets. The control component (5) includes a PLC controller and a display screen.

2. The low-temperature storage device for magnetic automatic sample retrieval according to claim 1, characterized in that... The upper storage disk (301), middle storage disk (302) and lower storage disk (303) are provided with regularly arranged storage holes. The storage well (3002) is located below the storage hole. The opening of the storage well (3002) is perpendicular to the storage hole. The storage well (3002) is made of aluminum alloy.

3. The low-temperature storage device for magnetic automatic sample retrieval according to claim 2, characterized in that... The rotating platform (401) is driven by its own rotating platform motor (401-1).

4. A magnetic suction-type automatic sample storage device according to any one of claims 1-3, characterized in that... The cross slide (402) includes a horizontal linear module (402-1) and a vertical linear module (402-2). The horizontal linear module (402-1) is a ball screw type linear module, which is driven by its own horizontal motor (402-1-2) to control the horizontal movement of its own horizontal slider (402-1-1). The vertical linear module (402-2) is a ball screw type linear module, which is driven by its own vertical motor (402-2-2) to control the vertical movement of its own vertical slider (402-2-1).

5. A magnetic suction-type automatic sample storage device according to claim 4, characterized in that... The input terminals of the outer rotary axis motor (202-1), the middle rotary axis motor (202-2), the inner rotary axis motor (202-3), the rotary platform motor (401-1), the horizontal motor (402-1-2), and the vertical motor (402-2-2) are connected to the output terminals of the PLC controller, and are controlled by the PLC controller.

Images