Movable embryo laboratory

Abstract

The utility model discloses a movable embryo laboratory which comprises a movable chassis and a laboratory box body, the laboratory box body is arranged above the movable chassis, and a traction device is fixedly arranged at the front end of the walking direction of the movable chassis. A unit operation and maintenance room, a disinfection room, an ovum collection room, an in-vitro fertilization culture room and a cryopreservation room are arranged in the laboratory box body from front to back in the walking direction of the movable chassis, and every two adjacent areas of the ovum collection room, the in-vitro fertilization culture room and the cryopreservation room are communicated through doors. Wall bodies of the disinfection room, the ovum collection room, the in-vitro fertilization culture room and the cryopreservation room are provided with delivery windows for communicating two adjacent areas, and the disinfection room is partitioned in the front-back direction to form a gas cylinder room and a decontamination room. According to the utility model, sperm and egg sample treatment time can be shortened, and embryo culture success rate is improved; sample loss caused by long-distance transportation is avoided; the operation flexibility is improved, and the embryo treatment cost is reduced; the method is convenient to deploy quickly and is suitable for pastures under different terrains and climate conditions.

Description

Technical fields:

[0001] This utility model relates to the field of livestock biological laboratory technology, specifically to a mobile embryo laboratory. Background technology:

[0002] In modern animal husbandry, embryo transfer technology is widely used for the rapid breeding of high-quality livestock. However, current embryo processing and culture procedures largely rely on stationary laboratories. These laboratories are typically located far from pastures, leading to the following problems.

[0003] Currently, the fixed laboratories for embryo processing and culture are located far apart, making transportation difficult: collected semen or egg samples need to be transported to fixed laboratories for processing, and cultured embryos need to be transported back to the farm for transfer. During this process, factors such as vibration, temperature, and environmental changes may cause a decline in sample quality; time delays: transportation and waiting processes prolong the sperm and egg processing time, reducing the success rate; high costs: transportation and processing require additional costs for cold chain logistics and transportation equipment. Establishing a fixed embryo laboratory in each farm would be extremely costly.

[0004] Therefore, there is an urgent need for a mobile laboratory that can be located near pastures and can be flexibly moved between different pastures, and can efficiently complete embryo processing and culture, in order to solve the above-mentioned shortcomings. Utility model content:

[0005] In view of this, the purpose of this utility model is to provide a mobile embryo laboratory that facilitates sperm and egg processing, embryo culture, cryopreservation, and pre-transfer embryo processing on a ranch.

[0006] The purpose of this utility model is achieved by the following technical solution: a mobile embryo laboratory, including a mobile chassis and a laboratory box, the laboratory box being installed above the mobile chassis, a traction device being fixedly provided at the front end of the mobile chassis in the direction of travel, the laboratory box having multiple functional areas, including, from front to back along the direction of travel of the mobile chassis, a unit maintenance room, a disinfection room, an oocyte collection room, an in vitro fertilization culture room, and a cryopreservation room, the oocyte collection room, the in vitro fertilization culture room, and the cryopreservation room, adjacent areas of which are connected by a connecting door, the walls of the disinfection room, the oocyte collection room, the in vitro fertilization culture room, and the cryopreservation room are provided with transfer windows connecting adjacent areas, the disinfection room being divided in the front-to-back direction to form a gas cylinder room and a washing and disinfection room.

[0007] Furthermore, the oocyte collection room has a changing room located only on the left side wall. A buffer area separates the changing room from the in vitro fertilization culture room. The buffer area is connected to the oocyte collection room, the changing room, and the in vitro fertilization culture room via a connecting door.

[0008] Furthermore, the right wall of the unit maintenance room and the gas cylinder room is provided with an entrance and exit that connects to the outside, the left wall of the decontamination room and the changing room is provided with an entrance and exit that connects to the outside, the left wall of the cryopreservation room is provided with a transfer window that connects to the outside, and the right wall of the egg collection room is also provided with a transfer window that connects to the outside.

[0009] Furthermore, the unit operation and maintenance room is equipped with a voltage stabilizer, power controller, clean air conditioning unit and water supply system to provide water and electricity for laboratory operation and maintain a clean environment of Class 10,000 cleanliness and positive pressure. The gas cylinder room contains several gas cylinders. The disinfection room is equipped with a sterilizer and dryer for sterilizing aseptic operation consumables and instruments. The oocyte collection room is equipped with a balance, osmometer, microscope and clean workbench. The in vitro fertilization culture room includes a centrifuge, incubator, refrigerator and in vitro fertilization workbench. The cryopreservation room includes a programmed freezer and liquid nitrogen tank.

[0010] Furthermore, the clean workbench is placed against the right wall of the egg collection room, and the clean workbench is connected to the outside through an equipment door.

[0011] Furthermore, the fertilization workbench is placed on the left wall of the in vitro fertilization culture room, and the in vitro fertilization workbench is connected to the outside through an equipment door.

[0012] Furthermore, the length and width dimensions of the laboratory enclosure are the same as those of the mobile chassis.

[0013] The advantages of this invention are: it greatly shortens the processing time of sperm and egg samples and improves the success rate of embryo culture when culturing, cryopreserving, and pre-transferring embryos in pastoral areas; it avoids sample loss caused by long-distance transportation; it improves operational flexibility and reduces the overall cost of embryo processing in livestock farms; it is easy to deploy quickly and is suitable for pastures with different terrains and climates. Attached image description:

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a top view of the structure of this utility model;

[0016] Figure 2 This is a schematic diagram of the left side structure of this utility model.

[0017] In the diagram, 1. Mobile chassis, 2. Laboratory enclosure, 21. Unit maintenance room, 211. Stabilized power supply, 212. Power controller, 213. Cleanroom air conditioning unit, 214. Water supply system, 22. Disinfection room, 221. Gas cylinder room, 2211. Gas cylinder, 222. Washing and disinfection room, 2221. Sterilizer, 2222. Dryer, 23. Oocyte collection room, 231. Balance, 232. Osmometer, 233. Microscope, 234. Clean workbench, 235. Buffer area, 236. Changing room, 24. In vitro fertilization culture room, 241. Centrifuge, 242. Incubator, 243. Refrigerator, 244. In vitro fertilization workbench, 25. Cryopreservation room, 251. Programmable freezer, 252. Liquid nitrogen tank, 26. Connecting door, 27. Pass-through window, 28. Exit door, 3. Traction device. Detailed implementation method:

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] like Figure 1-2 As shown, this embodiment provides a mobile embryo laboratory, including a mobile chassis 1 and a laboratory housing 2. The laboratory housing 1 is installed on top of the mobile chassis 1. The length and width of the laboratory housing 1 are the same as those of the mobile chassis 2. In this embodiment, the length of the laboratory housing 2 is 13716 mm, the width is 2980 mm, and the height is 2300 mm.

[0020] A traction device 3 is fixedly provided at the front end of the mobile chassis 1 in the direction of travel. The traction device 3 is a conventional connection structure. In this embodiment, one end of the traction device 3 is hinged to the mobile chassis 2, and the other end is connected to the traction vehicle by means of a pin.

[0021] The laboratory enclosure 2 has multiple functional areas, including, from front to back along the direction of travel of the mobile chassis 1, a unit maintenance room 21, a disinfection room 22, an oocyte collection room 23, an in vitro fertilization culture room 24, and a cryopreservation room 25. Adjacent areas of the oocyte collection room 23, the in vitro fertilization culture room 24, and the cryopreservation room 25 are connected by a connecting door 26. The walls of the disinfection room 22, the oocyte collection room 23, the in vitro fertilization culture room 24, and the cryopreservation room 25 are equipped with transfer windows 27 connecting adjacent areas. The disinfection room 22 is divided along the front and back direction to form a gas cylinder room 221 and a decontamination room 222. The area of ​​the decontamination room 222 is larger than that of the gas cylinder room 221. The unit operation and maintenance room 21 is equipped with a voltage stabilizer 211, a power controller 212, a clean air conditioning unit 213, and a water supply system 214, which are used to provide water and electricity for laboratory operation and maintain a clean environment with a cleanliness level of 10,000 and positive pressure in the room; it also includes a temperature control system (not shown in the figure) and a cleanliness control system (not shown in the figure) to ensure a stable and pollution-free environment for embryo processing; the dual power supply module and backup battery (not shown in the figure) can guarantee power supply and adapt to power needs in different scenarios. The gas cylinder room 221 contains several gas cylinders 2211, which are connected to various instruments via pipes to supply gas to them. The sterilization room 222 is equipped with a sterilizer 2221 and a dryer 2222 for sterilizing consumables and instruments used in aseptic operations. The oocyte collection room 23 is equipped with a balance 231, an osmometer 232, a microscope 233, and a clean bench 234, which maintains a Class 100 cleanliness level. The in vitro fertilization culture room 24 includes a centrifuge 241, an incubator 242, a refrigerator 243, and an in vitro fertilization workbench 244. The centrifuge 241 is used for frozen sperm processing, and the in vitro fertilization workbench 244 is used for oocyte in vitro fertilization operations. This equipment can provide a Class 100 cleanliness level while removing volatile organic compounds (VOCs) from the air. VOCs can seriously affect the survival and development of embryos. The incubator 242 is used for culturing embryos after fertilization. The cryopreservation room 25 includes a programmed cryostat 251 and a liquid nitrogen tank 252. The cryostat is used for the cooling process of sperm, eggs and embryos before cryopreservation, which can ensure that the frozen samples can continue to survive and develop after thawing. The liquid nitrogen tank 252 is used for the long-term preservation of samples after cooling by the programmed cryostat 251.

[0022] In a preferred embodiment, the oocyte collection room 23 has a changing room 236 located only on the left side wall. A buffer area 235 separates the changing room 236 from the in vitro fertilization culture room 24. The buffer area 235 is connected to the oocyte collection room 23, the changing room 236, and the in vitro fertilization culture room 24 through a connecting door 26.

[0023] The right wall of the unit maintenance room 21 and the gas cylinder room 221 has an access door 28 connecting to the outside. The left wall of the decontamination room 222 and the changing room 236 also has an access door 28 connecting to the outside. The left wall of the cryopreservation room 25 has a pass-through window 27 connecting to the outside. The right wall of the oocyte collection room 23 also has a pass-through window 27 connecting to the outside. A clean bench 234 is placed on the right wall of the oocyte collection room 23 and is connected to the outside through an equipment door. An in vitro fertilization workbench 244 is placed on the left side wall of the in vitro fertilization culture room 24 and is connected to the outside through an equipment door.

[0024] This embodiment can shorten the processing time of sperm and egg samples and improve the success rate of embryo culture when culturing, cryopreserving, and pre-transfer embryos in pastoral areas; avoid sample loss caused by long-distance transportation; improve operational flexibility and reduce the overall cost of embryo processing in livestock farms; facilitate rapid deployment and is suitable for pastures with different terrains and climates.

[0025] Cultivation process:

[0026] During embryo laboratory operation, the unit maintenance room 21 is responsible for providing power and water, and controlling the temperature, pressure, and cleanliness of the experimental area. The gas cylinder room 221 provides the gas required for equipment operation. Collected ovarian or live oocytes are transferred through the transfer window 27 to the oocyte collection room 23. After collection and selection in the clean bench 234, suitable oocyte samples are selected and transferred through the transfer window 27 to the in vitro fertilization culture room 24. Samples entering the in vitro fertilization culture room 24 undergo in vitro fertilization and in vitro culture development for a certain period under VOC-free Class 100 cleanliness and suitable temperature conditions. Embryos that have developed to a certain stage can be removed and transferred outside the laboratory through the transfer window 27 for transplantation into recipient animals. Embryos remaining after transplantation need to be cryopreserved for subsequent transplantation. These remaining embryos are transferred through the transfer window to the cryopreservation room 25. After processing by the programmed cryostat 251, the embryos are cooled from room temperature to approximately -180 degrees Celsius, close to liquid nitrogen. The cooled embryos are then transferred to the liquid nitrogen tank 252 for long-term preservation. Embryo samples in liquid nitrogen can be removed from liquid nitrogen tank 252 when transplantation is needed, placed in a water bath to rapidly heat up to 3 degrees Celsius to complete the revival, and the revival embryos can be transplanted into the mother animal.

[0027] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A mobile embryology laboratory, characterized in that, The utility model provides a mobile laboratory box, including mobile chassis and laboratory box, laboratory box is installed above mobile chassis, the front end of mobile chassis walking direction is fixed with traction device, the inside of laboratory box is equipped with a plurality of functional areas, including along mobile chassis walking direction from front to back respectively machine set operation room, disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room, two adjacent areas of ovum collection room, in vitro fertilization culture room and cryopreservation room are communicated through communicating door, the wall of disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room is equipped with the delivery window of communicating two adjacent areas, and the disinfection room is separated along the front and back direction and forms gas cylinder room and decontamination room.

2. A mobile embryology laboratory according to claim 1, characterised in that, The utility model discloses a mobile laboratory box, including mobile chassis and laboratory box, laboratory box is installed above mobile chassis, the front end of mobile chassis walking direction is fixed with traction device, the inside of laboratory box is equipped with a plurality of functional areas, including along mobile chassis walking direction from front to back respectively machine set operation room, disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room, two adjacent areas of ovum collection room, in vitro fertilization culture room and cryopreservation room are communicated through communicating door, the wall of disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room is equipped with the delivery window of communicating two adjacent areas, and the disinfection room is separated along the front and back direction and forms gas cylinder room and decontamination room.

3. A mobile embryology laboratory according to claim 2, wherein, The utility model discloses a mobile laboratory box, including mobile chassis and laboratory box, laboratory box is installed above mobile chassis, the front end of mobile chassis walking direction is fixed with traction device, the inside of laboratory box is equipped with a plurality of functional areas, including along mobile chassis walking direction from front to back respectively machine set operation room, disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room, two adjacent areas of ovum collection room, in vitro fertilization culture room and cryopreservation room are communicated through communicating door, the wall of disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room is equipped with the delivery window of communicating two adjacent areas, and the disinfection room is separated along the front and back direction and forms gas cylinder room and decontamination room.

4. A mobile embryology laboratory according to claim 3, wherein, The utility model discloses a mobile laboratory box, including mobile chassis and laboratory box, laboratory box is installed above mobile chassis, the front end of mobile chassis walking direction is fixed with traction device, the inside of laboratory box is equipped with a plurality of functional areas, including along mobile chassis walking direction from front to back respectively machine set operation room, disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room, two adjacent areas of ovum collection room, in vitro fertilization culture room and cryopreservation room are communicated through communicating door, the wall of disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room is equipped with the delivery window of communicating two adjacent areas, and the disinfection room is separated along the front and back direction and forms gas cylinder room and decontamination room.

5. A mobile embryology laboratory according to claim 4, wherein, The utility model discloses a mobile laboratory box, including mobile chassis and laboratory box, laboratory box is installed above mobile chassis, the front end of mobile chassis walking direction is fixed with traction device, the inside of laboratory box is equipped with a plurality of functional areas, including along mobile chassis walking direction from front to back respectively machine set operation room, disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room, two adjacent areas of ovum collection room, in vitro fertilization culture room and cryopreservation room are communicated through communicating door, the wall of disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room is equipped with the delivery window of communicating two adjacent areas, and the disinfection room is separated along the front and back direction and forms gas cylinder room and decontamination room.

6. A mobile embryology laboratory according to claim 4, wherein, The utility model discloses a mobile laboratory box, including mobile chassis and laboratory box, laboratory box is installed above mobile chassis, the front end of mobile chassis walking direction is fixed with traction device, the inside of laboratory box is equipped with a plurality of functional areas, including along mobile chassis walking direction from front to back respectively machine set operation room, disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room, two adjacent areas of ovum collection room, in vitro fertilization culture room and cryopreservation room are communicated through communicating door, the wall of disinfection room, ovum collection room, in vitro fertilization culture room and cryopreservation room is equipped with the delivery window of communicating two adjacent areas, and the disinfection room is separated along the front and back direction and forms gas cylinder room and decontamination room.

7. A mobile embryology laboratory according to claim 2, wherein, ​

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