Subpackaging device and subpackaging equipment for cell freezing medium
By designing a dispenser that includes a flow guide tube and an elastic membrane valve, the problem of low efficiency in manual dispensing was solved, achieving efficient and stable dispensing of cell cryopreservation solution and ensuring cell quality and equipment reliability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- ZHEJIANG TIANYUAN BIOTECHNOLOGY CO LTD
- Filing Date
- 2025-04-29
- Publication Date
- 2026-04-21
AI Technical Summary
In existing technologies, manual dispensing of cell cryopreservation solutions is inefficient and affects cell quality during high-throughput cryopreservation.
Design a dispenser comprising a main body, multiple guide tubes and an elastic sealing assembly. An elastic diaphragm valve is provided below the guide tubes, which enables automatic opening and closing. Combined with a fixing ring and lug structure, it ensures stability and accurate dispensing.
It enables efficient and simple multiple dispensing, ensuring cell quality, reducing operational difficulty, extending equipment lifespan, and reducing dispensing errors.
Smart Images
Figure CN224147715U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of biological sample processing technology, and more specifically, to a dispenser and dispensing device for cell cryopreservation solution. Background Technology
[0002] Cell cryopreservation is a key technology for the long-term preservation of biological samples. The dispensing process must strictly ensure sterility, operational efficiency, and the accuracy of cryopreservation solution dosage. Traditional dispensing methods mainly rely on manual operation, such as using pipettes or droppers to dispense the cryopreservation tubes one by one.
[0003] However, the relevant technologies have at least one of the following problems: manual dispensing in the prior art requires repeated aspiration and drainage of liquid, which is inefficient and will affect cell quality when dealing with high-throughput cryopreservation needs. Utility Model Content
[0004] The technical problem solved by this invention is that in the prior art, manual dispensing requires repeated aspiration and drainage of liquid, which is inefficient and affects cell quality when handling high-throughput cryopreservation needs.
[0005] To address the aforementioned problems, this utility model provides a dispenser for cell cryopreservation solution. The dispenser includes: a main body with a receiving space, and multiple guide tubes connected to the main body at its lower end. The lower end of each guide tube has a first opening, and the receiving space and the first opening are connected through a first inner cavity of the guide tube; an elastic sealing assembly located below the guide tubes, and an elastic membrane valve corresponding to the first opening. When the guide tubes are pressed down to the elastic membrane valve, the elastic membrane valve opens under pressure, forming a first channel for the cell cryopreservation solution to flow through; when the guide tubes no longer apply pressure to the elastic membrane valve, the elastic membrane valve automatically closes.
[0006] Compared with existing technologies, the technical effects achieved by adopting this technical solution are as follows: by setting multiple guide tubes below the main body and elastic diaphragm valves that cooperate with the guide tubes, multiple dispensing can be performed at one time, which improves efficiency and is simple to operate and convenient for staff to use.
[0007] In one embodiment of this utility model, a cryopreservation box is also included, which contains a plurality of cryopreservation tubes that cooperate with the guide tubes. The positions of the tube openings of the cryopreservation tubes correspond one-to-one with the guide tubes. When the guide tubes cooperate with the cryopreservation tubes, the guide tubes are inserted into the cryopreservation tubes, and the first opening of the guide tubes communicates with the second inner cavity of the cryopreservation tubes.
[0008] Compared with existing technologies, the technical effects achieved by adopting this technical solution are as follows: by matching the position of the cryopreservation tube with the flow tube, multiple flow tubes can be dispensed at one time, which not only improves efficiency, but also ensures cell quality due to the shortened time.
[0009] In one embodiment of this utility model, a fixing ring is provided at one end of the elastic sealing assembly near the guide tube, and the fixing ring is sleeved on the outside of the guide tube; wherein, the fixing ring can slide with the outer wall of the guide tube so that the guide tube can move relative to the elastic diaphragm valve.
[0010] Compared with existing technologies, the technical advantages achieved by this solution are as follows: The sliding fit design between the retaining ring and the guide tube optimizes the movement stability and sealing reliability of the guide tube. The retaining ring, sleeved on the outside of the guide tube, provides axial guidance during the pressing process, preventing localized wear or sealing failure of the elastic diaphragm valve caused by misalignment. Furthermore, the sliding fit structure facilitates quick assembly and disassembly of the guide tube and retaining ring, simplifies cleaning and maintenance procedures, and extends the service life of the dispenser.
[0011] In one embodiment of this utility model, the outer wall of the fixing ring is provided with a lug; wherein, when the guide tube and the cryopreservation tube are engaged, the lug abuts against the tube opening.
[0012] Compared with the existing technology, the technical effect achieved by adopting this technical solution is as follows: by setting a lug on the outside of the fixed ring, when the guide tube is pressed down, the lug is limited at the opening of the cryopreservation tube, and the elastic membrane valve cannot move further downward, which facilitates the insertion of the guide tube into the elastic membrane valve and opens the first channel.
[0013] In one embodiment of this utility model, the upper half of the guide tube is a tapered section, and the lower half of the guide tube is a straight section; wherein, the outer wall of the straight section is provided with a first limiting ring, and the outer diameter of the first limiting ring is larger than the inner diameter of the fixing ring.
[0014] Compared with existing technologies, the technical effects achieved by this technical solution are as follows: the outer diameter of the first limiting ring is larger than the inner diameter of the fixed ring, forming a stop and reducing the dispensing error caused by differences in operating force.
[0015] In one embodiment of this utility model, the end of the straight pipe section away from the tapered pipe section is provided with an opening valve element that cooperates with the elastic diaphragm valve so that the elastic diaphragm valve is opened under pressure.
[0016] Compared with existing technologies, the technical effect achieved by adopting this technical solution is: by setting an opening valve at the end of the straight pipe section, it is convenient for the flow guide pipe to open the elastic diaphragm valve.
[0017] In one embodiment of this utility model, the dispenser further includes a first cap, which is located above the main body and has an inlet communicating with the receiving space. The first cap includes a pressing part with anti-slip texture. When pressure is applied to the pressing part, the guide tube moves toward the elastic diaphragm valve to open the elastic diaphragm valve.
[0018] Compared with existing technologies, the technical effects achieved by this solution are as follows: the mixed cell cryopreservation solution can enter the containment space through the inlet, facilitating dispensing; the anti-slip textured pressing part on the first cap facilitates stable pressure application even when wearing gloves or in humid environments, further reducing operational difficulty and improving dispensing efficiency and reliability. Furthermore, this invention also provides a dispensing device, including a dispenser for cell cryopreservation solution as described in any of the above examples. The dispensing device further includes: a mixing device for uniformly pre-treating the cell cryopreservation solution before dispensing, and transferring the pre-treated cell cryopreservation solution to the containment space; wherein the dispensing device dispenses the mixed cell cryopreservation solution into cryovials via the dispenser.
[0019] Compared with existing technologies, the technical effects achieved by adopting this technical solution are as follows: it can achieve the technical effects corresponding to any of the above examples, which will not be elaborated here.
[0020] By adopting the technical solution of this utility model, the following technical effects can be achieved:
[0021] (1) By setting multiple guide tubes below the main body and elastic diaphragm valves that cooperate with the guide tubes, multiple dispensing can be performed at one time, which improves efficiency and is simple to operate and convenient for staff to use.
[0022] (2) By matching the position of the cryopreservation tube with the flow tube, multiple flow tubes can be dispensed at once, which improves efficiency and ensures cell quality due to the shortened time.
[0023] (3) By setting a lug on the outside of the fixed ring, when the guide tube is pressed down, the lug is limited at the opening of the cryopreservation tube, and the elastic membrane valve cannot move further down, which makes it convenient for the guide tube to be inserted into the elastic membrane valve and open the first channel. Attached Figure Description
[0024] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings to be used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0025] Figure 1 A schematic diagram of the structure of a dispenser provided in an embodiment of this utility model;
[0026] Figure 2 A schematic diagram of the structure of a cryopreservation box provided in an embodiment of this utility model;
[0027] Figure 3 A schematic diagram of the structure of the guide tube and cryopreservation tube when they are used together, as provided in an embodiment of this utility model;
[0028] Figure 4 for Figure 3 Schematic diagram of the cross-sectional structure along the AA direction;
[0029] Figure 5 for Figure 3 A schematic diagram of the explosion structure.
[0030] Explanation of reference numerals in the attached figures:
[0031] 1. Dispenser; 10. Body; 11. First cap; 12. Pressing part; 13. Liquid inlet; 20. Guide tube; 21. First opening; 22. First inner cavity; 23. First limiting ring; 24. Valve opening component; 30. Elastic sealing assembly; 31. Elastic diaphragm valve; 32. Fixing ring; 33. Lug; 40. Cryopreservation box; 41. Cryopreservation tube; 42. Second inner cavity. Detailed Implementation
[0032] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.
[0033] See Figures 1 to 5 This invention provides a dispenser 1 for cell cryopreservation solution. The dispenser 1 includes a body 10 and an elastic sealing assembly 30. The body 10 has a receiving space inside, and multiple guide tubes 20 connected to the body 10 are provided below the body 10. The lower end of the guide tube 20 has a first opening 21, and the receiving space and the first opening 21 are connected through the first inner cavity 22 of the guide tube 20. The elastic sealing assembly 30 is located below the guide tubes 20, and an elastic membrane valve 31 is provided at the location of the elastic sealing assembly 30 corresponding to the first opening 21. When the guide tube 20 is pressed down to the elastic membrane valve 31, the elastic membrane valve 31 is opened under pressure, forming a first channel for the cell cryopreservation solution to flow. When the guide tube 20 no longer applies pressure to the elastic membrane valve 31, the elastic membrane valve 31 automatically closes.
[0034] By setting multiple guide tubes 20 below the main body 10, and elastic diaphragm valves 31 that cooperate with the guide tubes 20, multiple dispensing can be performed at once, which improves efficiency and is simple to operate and convenient for staff to use.
[0035] Preferably, the number of guide tubes 20 is determined according to the usage, such as 48, 81 or 96 guide tubes 20; the volume of the first inner cavity 22 of the guide tube 20 is 1mL to 5mL, depending on the needs of the user during production.
[0036] Furthermore, the dispensing device 1 in this embodiment is made of plastic and is a disposable device, which eliminates the need for cleaning and makes it easier for production personnel to use.
[0037] Preferably, the elastic diaphragm valve 31 is made of an elastic material, such as rubber or silicone, so that the elastic diaphragm valve 31 can close automatically.
[0038] Specifically, the elastic diaphragm valve 31 can be represented by a cross-shaped cut on a rubber diaphragm so that the elastic diaphragm valve 31 can open the first channel when it is subjected to pressure from the guide tube 20.
[0039] Preferably, the elastic sealing assembly 30 can be multiple separate devices, each elastic sealing assembly 30 cooperating with the guide pipe 20; the elastic sealing assembly 30 can also be a whole baffle, with an elastic diaphragm valve 31 provided at the position corresponding to the guide pipe 20, to facilitate manufacturing.
[0040] Furthermore, this application also includes a cryopreservation box 40, which has a plurality of cryopreservation tubes 41 that cooperate with the guide tube 20. The positions of the tube openings of the cryopreservation tubes 41 correspond one-to-one with the guide tubes 20. When the guide tube 20 cooperates with the cryopreservation tubes 41, the guide tube 20 is inserted into the cryopreservation tubes 41, and the first opening 21 of the guide tube 20 is connected to the second inner cavity 42 of the cryopreservation tube 41.
[0041] By matching the position of the cryopreservation tube 41 with that of the flow tube 20, multiple flow tubes 20 can be dispensed at once, which improves efficiency and ensures cell quality due to the shortened time.
[0042] Preferably, the elastic sealing assembly 30 is provided with a fixing ring 32 at one end near the guide tube 20, and the fixing ring 32 is sleeved on the outside of the guide tube 20; wherein, the fixing ring 32 can slide with the outer wall of the guide tube 20 so that the guide tube 20 can move relative to the elastic diaphragm valve 31.
[0043] The sliding fit design between the retaining ring 32 and the guide tube 20 optimizes the movement stability and sealing reliability of the guide tube 20. The retaining ring 32, sleeved on the outside of the guide tube 20, provides axial guidance during the pressing process of the guide tube 20, preventing localized wear or sealing failure of the elastic diaphragm valve 31 due to misalignment. Furthermore, the sliding fit structure facilitates quick assembly and disassembly of the guide tube 20 and the retaining ring 32, simplifying cleaning and maintenance procedures and extending the service life of the dispenser 1.
[0044] Preferably, the retaining ring 32 is made of a rigid material so that the elastic sealing assembly 30 can be installed on the guide tube 20.
[0045] Specifically, the outer wall of the fixing ring 32 is provided with a lug 33; wherein, when the guide tube 20 is engaged with the cryopreservation tube 41, the lug 33 abuts against the tube opening. By providing a lug 33 on the outer side of the fixing ring 32, when the guide tube 20 is pressed down, the lug 33 is limited at the tube opening of the cryopreservation tube 41, and the elastic diaphragm valve 31 cannot move further downward, which facilitates the insertion of the guide tube 20 into the elastic diaphragm valve 31 and the opening of the first channel.
[0046] Furthermore, the lug 33 can be multiple long strip-shaped rigid parts, with at least two lugs 33 hanging on the opening of the cryopreservation tube 41 to prevent the elastic sealing assembly 30 from moving further downward; the lug 33 can also be set as a limiting ring surrounding the fixing ring 32, with the outer diameter of the limiting ring being larger than the inner diameter of the opening of the cryopreservation tube 41.
[0047] Furthermore, the upper half of the flow guide tube 20 is a tapered section, and the lower half is a straight section. This segmented design of the tapered and straight sections of the flow guide tube 20 optimizes the flow properties and structural compatibility of the cell cryopreservation solution. The tapered section's wider upper section and narrower lower section accelerates the flow of liquid from the containment space of the main body 10 to the straight section, reducing residual liquid volume; the straight section forms a stable connection with the inner wall of the cryopreservation tube 41, avoiding dosage errors caused by liquid turbulence during dispensing.
[0048] Furthermore, a first limiting ring 23 is provided on the outer wall of the straight pipe section; wherein, the outer diameter of the first limiting ring 23 is larger than the inner diameter of the fixed ring 32. The outer diameter of the first limiting ring 23 is larger than the inner diameter of the fixed ring 32, forming a mechanical stop to prevent the guide pipe 20 from being excessively pressed down, which could cause permanent deformation or damage to the elastic diaphragm valve 31. This structure ensures that the displacement of the guide pipe 20 is consistent each time it is dispensed, thereby ensuring the repeatability of the dispensing dosage and reducing dispensing errors caused by differences in operating force.
[0049] Preferably, the end of the straight pipe section away from the tapered pipe section is provided with an opening valve element 24 that cooperates with the elastic diaphragm valve 31, so that the elastic diaphragm valve 31 can be opened under pressure. By providing the opening valve element 24 at the end of the straight pipe section, it is convenient for the guide pipe 20 to open the elastic diaphragm valve 31.
[0050] Preferably, the dispenser 1 further includes a first cap 11, which is positioned above the main body 10. The first cap 11 has a liquid inlet 13 communicating with the containment space. The first cap 11 covers the main body 10, preventing external contaminants from entering the containment space. The liquid inlet 13 allows direct filling of cryopreservation solution without disassembling the main body 10, avoiding the risk of secondary contamination. Furthermore, the mixed cell cryopreservation solution can enter the containment space through the liquid inlet 13, facilitating dispensing.
[0051] Furthermore, the first cover 11 includes a pressing part 12, which has anti-slip texture; wherein, when pressure is applied to the pressing part 12, the guide tube 20 moves toward the elastic diaphragm valve 31 so that the elastic diaphragm valve 31 opens.
[0052] The anti-slip textured pressing part 12 on the first cap 11 makes it easy to apply pressure stably when wearing gloves or in a humid environment, further reducing the difficulty of operation and improving the efficiency and reliability of dispensing.
[0053] On the other hand, the present invention also provides a dispensing device, including a dispenser 1 for cell cryopreservation solution as described in any of the above examples. The dispensing device further includes: a mixing device for uniformly pre-treating the cell cryopreservation solution before dispensing, wherein the pre-treated cell cryopreservation solution is transferred to a receiving space; wherein the dispensing device dispenses the mixed cell cryopreservation solution into cryopreservation tubes 41 via the dispenser 1.
[0054] Preferably, the mixing device in this embodiment includes a mixing container and a dispensing container. The mixing container has an arc-shaped outer wall to facilitate uniform mixing of the cell cryopreservation solution. The dispensing container can be a measuring cup. The volume of the mixing container is 1-2L, and the volume of the dispensing container is 50mL-100mL.
[0055] In one specific embodiment, the cell cryopreservation solution is first mixed evenly in a mixing container, and then poured into a dispensing container corresponding to the dispenser 1. The user then pours the cell cryopreservation solution in the dispensing container into the holding space through the inlet 13. The user can gently shake the container to evenly distribute the cell cryopreservation solution into the first inner cavity 22 of multiple guide tubes 20. Subsequently, the user aligns the dispenser 1 and the cryopreservation box 40 so that each guide tube 20 is inserted into the cryopreservation tube 41, and then presses the pressing part 12 on the first cap 11. At this time, the lug 33 abuts against the opening of the cryopreservation tube 41, preventing the elastic membrane valve 31 from moving downward. The guide tube 20 moves downward, causing the elastic membrane valve 31 to open under pressure, and the cell cryopreservation solution in the first inner cavity 22 of the guide tube 20 flows into the cryopreservation tube 41.
[0056] In addition, after the dispenser 1 completes the dispensing, the cryopreservation tube 41 can be packaged using a multi-row or single-row automatic packaging machine.
[0057] While the present invention has been disclosed above, it is not limited thereto. Any person skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention; therefore, the scope of protection of the present invention should be determined by the scope defined in the claims.
Claims
1. A dispenser for cell cryopreservation solutions, characterized in that, The dispenser includes: The body (10) has a receiving space. The body (10) has a plurality of guide tubes (20) connected to the body (10) at its lower end. The lower end of the guide tube (20) has a first opening (21). The receiving space and the first opening (21) are connected through the first inner cavity (22) of the guide tube (20). An elastic sealing assembly (30) is provided below the guide tube (20), and an elastic diaphragm valve (31) is provided on the elastic sealing assembly (30) at the location corresponding to the first opening (21); When the guide tube (20) is pressed down to the elastic membrane valve (31), the elastic membrane valve (31) is opened under pressure, forming a first channel for the flow of the cell cryopreservation solution; When the flow guide (20) no longer applies pressure to the elastic diaphragm valve (31), the elastic diaphragm valve (31) automatically closes.
2. The dispenser for cell cryopreservation solution according to claim 1, characterized in that, It also includes a cryopreservation box (40), which has a plurality of cryopreservation tubes (41) that cooperate with the guide tube (20), and the position of the tube opening of the cryopreservation tube (41) corresponds one-to-one with the guide tube (20); When the guide tube (20) is used in conjunction with the cryopreservation tube (41), the guide tube (20) is inserted into the cryopreservation tube (41), and the first opening (21) of the guide tube (20) is connected to the second inner cavity (42) of the cryopreservation tube (41).
3. The dispenser for cell cryopreservation solution according to claim 2, characterized in that, The elastic sealing assembly (30) has a fixing ring (32) at one end near the guide tube (20), and the fixing ring (32) is sleeved on the outside of the guide tube (20); The fixing ring (32) can slide against the outer wall of the guide tube (20) so that the guide tube (20) can move relative to the elastic diaphragm valve (31).
4. The dispenser for cell cryopreservation solution according to claim 3, characterized in that, The outer side wall of the fixing ring (32) is provided with a lug (33); When the guide tube (20) is engaged with the cryopreservation tube (41), the lug (33) abuts against the tube opening.
5. The dispenser for cell cryopreservation solution according to claim 3, characterized in that, The upper part of the guide pipe (20) is a tapered section, and the lower part of the guide pipe (20) is a straight section; The outer wall of the straight pipe section is provided with a first limiting ring (23), and the outer diameter of the first limiting ring (23) is larger than the inner diameter of the fixed ring (32).
6. The dispenser for cell cryopreservation solution according to claim 5, characterized in that, The straight pipe section is provided with an opening valve (24) that cooperates with the elastic diaphragm valve (31) at one end away from the tapered pipe section, so that the elastic diaphragm valve (31) is opened under pressure.
7. The dispenser for cell cryopreservation solution according to claim 1, characterized in that, The dispenser also includes a first cap (11), which is placed on top of the body (10). The first cap (11) has a liquid inlet (13) that communicates with the containing space. The first cover (11) includes a pressing part (12), and the pressing part (12) is provided with anti-slip texture; When pressure is applied to the pressing part (12), the guide tube (20) moves toward the elastic diaphragm valve (31) to open the elastic diaphragm valve (31).
8. A dispensing apparatus comprising a dispenser for cell cryopreservation solution as described in any one of claims 1-7, characterized in that, The packaging equipment also includes: A mixing device is used to uniformly pretreat the cell cryopreservation solution before dispensing, and the pretreated cell cryopreservation solution is transferred to the containing space. The dispensing device dispenses the mixed cell cryopreservation solution into the cryopreservation tube (41) via the dispenser.