Mounting a sample separation unit by a rotating mechanism

Abstract

A mounting device (100) for mounting a sample separation unit (30) configured to separate, preferably chromatographically separate, a compound in a fluid sample, wherein the mounting device (100) comprises a first fluidic connector (102) configured to be mechanically and fluidically coupled with a first fluidic interface (104) of the sample separation unit (30), a second fluidic connector (106) configured to be mechanically and fluidically coupled with a second fluidic interface (108) of the sample separation unit (30), and a rotation mechanism (110) configured to rotate the first fluidic connector (102) between a mounting orientation for mounting the first fluidic interface (104) of the sample separation unit (30) at the first fluidic connector (102) and an alignment orientation for aligning the second fluidic interface (108) of the mounted sample separation unit (30) with the second fluidic connector (106) for subsequently coupling the second fluidic interface (108) with the second fluidic connector (106).

Description

Technical Field

[0001] This invention relates to an installation unit, a sample separation device, and a method for installing the sample separation unit. Background Technology

[0002] In liquid chromatography, a fluid (such as a mixture between a fluid sample and a mobile phase) can be pumped through a conduit and a column comprising a material (stationary phase) capable of separating the different components of the fluid sample. This material, such as so-called beads that may include silica gel, can be packed into the column, which can be connected via conduits to other components such as sampling units, flow cells, sample containers, and / or buffers.

[0003] During the separation process, it may be necessary or desirable for the column to reach a desired temperature. For this purpose, the column is mounted in a column oven and heated there. Mounting the column in a column oven is typically uncomfortable for the user. During such assembly, the user must routinely handle small and sensitive components, such as capillaries and fittings, and fluidly couple them to the column. This is a delicate activity that is not easily performed by the user without specialized expertise. Summary of the Invention

[0004] The object of this invention is to enable the simple and reliable installation of the sample separation unit. This object is achieved through the independent claims. Other embodiments are illustrated in the dependent claims.

[0005] According to an exemplary embodiment of the present invention, an installation device for mounting a sample separation unit is provided, the sample separation unit being configured to separate, preferably chromatographically separate, complexes in a fluid sample (which may also be referred to as a sample fluid), wherein the installation device includes: a first fluid connector configured to be mechanically and fluidly coupled to a first fluid interface (e.g., an inlet) of the sample separation unit; a second fluid connector configured to be mechanically and fluidly coupled to a second fluid interface (e.g., an outlet) of the sample separation unit; and a rotation mechanism (which may also be referred to as a pivoting mechanism) configured to rotate (or pivot) the first fluid connector between an installation orientation (particularly the rotation mechanism, the first fluid connector, the sample separation unit, and / or the installation device) and an alignment orientation (particularly the rotation mechanism, the first fluid connector, the sample separation unit, and / or the installation device), the installation orientation for mounting the first fluid interface of the sample separation unit at the first fluid connector, and the alignment orientation for aligning the second fluid interface of the mounted sample separation unit with the second fluid connector for subsequently coupling the second fluid interface to the second fluid connector.

[0006] According to another exemplary embodiment of the present invention, a sample separation device for separating fluid samples is provided, wherein the sample separation device includes a fluid drive unit configured to drive a mobile phase and a fluid sample injected into the mobile phase, a sample separation unit configured to separate the fluid sample, and a mounting device having the above-described features, the mounting device being used to mount the sample separation unit at the sample separation device.

[0007] According to yet another exemplary embodiment, a method for mounting a sample separation unit configured to separate, preferably chromatographically separate, complexes in a fluid sample is provided, wherein the method includes: mounting a first fluid interface of the sample separation unit at a first fluid connector in a mounting orientation to mechanically and fluidly couple the first fluid connector to the first fluid interface of the sample separation unit; then rotating a rotation mechanism from the mounting orientation to an alignment orientation to align a second fluid interface of the sample separation unit with a second fluid connector; and in the alignment orientation, mechanically and fluidly coupling the second fluid connector to the second fluid interface of the sample separation unit.

[0008] In the context of this application, the term "fluid connector" can specifically refer to a component configured to form a fluid (and particularly fluid-tight, more particularly high-pressure-tight) connection with the inlet or outlet of a sample separation unit. In particular, such a fluid connector can be presented as an accessory.

[0009] In the context of this application, the term "fluid interface" may specifically refer to the inlet or outlet section of a sample separation unit (such as a chromatographic column). Such a fluid interface may be adapted to establish a fluid connection with a fluid connector of a mounting device.

[0010] In the context of this application, the term "mechanically and fluidly coupled" may specifically refer to establishing a connection between a corresponding fluid connector and a corresponding fluid interface, wherein fluid communication is established between the fluid connector and the sample separation unit.

[0011] In the context of this application, the term "rotating first fluid connector" can specifically refer to the action of pivoting a pivotally mounted first fluid connector (particularly together with a sample separation unit mounted on the first fluid connector) between a predetermined orientation (particularly a mounting orientation and an alignment orientation). Preferably, the sample separation unit can be connected to the first fluid connector and can act as a rotating rod during such rotational operation.

[0012] In the context of this application, the term "installation orientation" can specifically refer to a defined angular orientation (which may differ from alignment or analysis orientation) in which a user has access to the first fluid connector, allowing the user to easily (preferably without tools, i.e., in a tool-free manner) install the sample separation unit onto the first fluid connector in the installation orientation, or remove the sample separation unit installed on the first fluid connector from the mounting device. In the installation orientation, the sample separation unit installed at the mounting device may not yet be coupled to its two fluid interfaces at the two fluid connectors of the mounting device for fluid communication or even in a fluid-tight manner. This allows the user in the installation orientation to insert or remove the sample separation unit from the mounting device. In embodiments, the first fluid connector may be biased in the installation orientation (e.g., due to the fluid connector's own weight, and / or by a biasing unit such as a bias spring or bias magnet), i.e., it may exist in the installation orientation without external force. As a result, the installation orientation can be the default orientation of the mounting device.

[0013] In the context of this application, the term "alignment orientation" can specifically refer to a defined angular orientation (which may differ from the mounting orientation) in which the first fluid connector and the sample separation unit mounted on the first fluid connector are aligned with the second fluid connector. Specifically, this alignment can cause the second fluid connector to move toward, or conversely toward, the first fluid connector with the assembled sample separation unit, a subsequent predetermined or guided (particularly longitudinal) movement, or vice versa, to allow the arrangement of the fluid connector and the sample separation unit into an analytical or separation configuration in which a sample separation process using the sample separation unit can be performed. When in alignment orientation (and preferably also in a certain position), the first fluid connector and the sample separation unit can be in an orientation that readily prepares the first fluid connector and the sample separation unit for sample separation or analysis.

[0014] In the context of this application, the term "sample separation apparatus" can specifically refer to an apparatus configured to separate a fluid sample into, for example, different fractions. In particular, the sample separation apparatus can be a chromatographic apparatus. When a fluid sample is supplied to the sample separation apparatus and injected by an injector into the separation path between the fluid drive unit and the sample separation unit, the different physical, chemical, and / or biological properties of the different fractions of the fluid sample can lead to the separation of the different fractions in the sample separation unit.

[0015] In the context of this application, the term "fluid sample" (or sample fluid) can specifically refer to any liquid and / or gaseous medium, and optionally also includes solid particles to be separated. Such fluid samples can include a variety of different molecules or particles to be separated, such as small molecules or large biomolecules such as proteins. The separation of fluid samples may involve certain separation criteria (such as mass, volume, chemical properties, etc.) according to which separation is performed.

[0016] In the context of this application, the term "mobile phase" can specifically refer to any liquid and / or gaseous medium that can be used as a fluid carrier for a fluid sample during separation. The mobile phase can be a solvent or solvent composition (e.g., composed of water and an organic solvent such as ethanol or acetonitrile). In isocratic separation mode of a liquid chromatography apparatus, the mobile phase can have a composition that remains constant over time. However, in gradient mode, the composition of the mobile phase can change over time, particularly to desorb fractions of the fluid sample that have previously been adsorbed onto the stationary phase of the sample separation unit.

[0017] In the context of this application, the term "fluid drive unit" may specifically refer to an entity capable of driving fluids (i.e., liquids and / or gases, optionally including solid particles), particularly fluid samples and / or mobile phases. For example, a fluid drive may be a pump (e.g., embodied as a piston pump or peristaltic pump) or another pressure source. For example, a fluid drive unit may be a high-pressure pump, such as one capable of driving fluids at pressures of at least 100 bar, particularly at least 1000 bar.

[0018] The term "sample separation unit" can specifically refer to a fluid component through which a fluid sample is transferred, and which is configured such that, as the fluid sample is guided through the separation unit, it will be separated into different groups of molecules or particles. An example of a separation unit is a liquid chromatography column capable of collecting or retaining and selectively releasing different fractions of a fluid sample. In particular, the sample separation unit can be a tubular body with an aspect ratio (i.e., the ratio of length to diameter) greater than 1, especially greater than 2, for example, even at least 3. For example, the length of a sample separation unit can range from 80 mm to 300 mm, for example, 100 mm.

[0019] According to an exemplary embodiment, an installation device is provided for establishing a user-friendly and reliable mechanical and fluid connection, for example, a sample separation unit presented as a chromatographic separation column. With this installation device, after the user mounts the sample separation unit onto a first fluid connector in an angular mounting orientation, the first fluid connector can be intuitively rotated together with the sample separation unit to rotate or pivot the sample separation unit from the mounting orientation to an alignment orientation, in which the first fluid connector and the sample separation unit are pre-aligned with a second fluid connector for fluid coupling to prepare the sample separation device for sample analysis or separation. Thus, the mounting orientation can correspond to the entry position and orientation of the first fluid connector and the sample separation unit, while the alignment orientation can correspond to the analysis position and orientation of the sample separation unit. Advantageously, the first fluid connector can be presented to the user in a mounting orientation to allow for anatomically advantageous assembly of the sample separation unit in a user-friendly manner. The intuitive and simple rotational movement of the sample separation unit together with the first fluid connector can then be converted into an alignment orientation, in which it is aligned with the second fluid connector. The assembly of the sample separation unit in the installation device can then be completed by bringing the second fluid connector and the sample separation unit closer together. The second fluid connector can be connected, for example, manually or automatically, via mechanical mechanisms (e.g., knee levers), motorized mechanisms, hydraulic mechanisms, and / or pneumatic devices. Such an assembly process is simple and robust against failure. In a preferred embodiment, simply rotating the sample separation unit to an alignment orientation simultaneously results in a sealed (preferably high-pressure sealed) fluid coupling between the sample separation unit and the first fluid connector of the mounting device. Described, the sample separation unit itself can act as a rotating rod, which operates a sealing eccentric element, etc., triggered only by a rotational action. Apart from mounting the sample separation unit on the first fluid connector and performing an intuitive rotational action, the user does not need to do anything else to prepare the sample separation unit for subsequent fluid coupling with the second fluid connector. This allows even users without specialized knowledge to reliably assemble the sample separation unit in the sample separation apparatus. Using the mounting device according to exemplary embodiments of the invention, time-consuming, delicate, and error-prone operations such as those involving capillaries and their connection to the sample separation unit can be eliminated. Therefore, the mounting device enables rapid, reliable, and user-friendly assembly of the sample separation unit in the sample separation apparatus. After the sample separation unit is assembled onto the mounting device, accurate separation results can be obtained because the sample separation unit in the sample separation device reliably forms fluid coupling. Furthermore, the column holder, as well as the user's handling and connection of capillaries and tools, become unnecessary.

[0020] Further embodiments of the installation apparatus, sample separation equipment, and method will be explained below.

[0021] In this embodiment, the first fluid connector is a tapered fluid connector for insertion into a first fluid interface of the sample separation unit. Additionally or alternatively, the second fluid connector may be a tapered fluid connector for insertion into a second fluid interface of the sample separation unit. More generally, either the first or second fluid connector may be a male fluid connector. Such a male fluid connector can mate with a corresponding female fluid interface of the sample separation unit.

[0022] In an embodiment, at least one of the first and second fluid connectors is configured to move toward a corresponding other fluid connector for coupling the second fluid interface of the sample separation unit to the second fluid connector during alignment. Specifically, the first fluid connector may be longitudinally stationary, while the second fluid connector may move along a defined longitudinal axis. The configuration with at least one movable fluid connector allows the distance between the fluid connectors to be adjusted according to the length of the sample separation unit to be mounted. This makes it possible to use the same mounting device for different sample separation units, which can be freely selected over a wide range of length values. For example, the mounting device may be configured to support the sample separation unit for a length range of at least 30 mm to 300 mm (e.g., length values ​​of 30 mm, 50 mm, 80 mm, or 100 mm are possible). This increases the user's flexibility in using a universal mounting device.

[0023] In an embodiment, at least one movable component of the first and second fluid connectors is connected to a longitudinal guide unit to enable guided movement (particularly sliding) along the longitudinal direction of motion, particularly along a track. For example, the track may be arranged coaxially or parallel to the sample separation unit. Accordingly, the process of mechanically and fluidly coupling the second fluid connector to the second fluid interface during alignment and orientation may include sliding or pushing the second fluid connector onto the second fluid interface. For example, the distance between the fluid connectors may be freely adjustable in a continuous or stepwise manner.

[0024] In this embodiment, the alignment orientation is parallel to the longitudinal direction of movement. Furthermore, the mounting orientation can be tilted relative to the longitudinal sliding direction. Therefore, when the sample separation unit and the first fluid connector are in the alignment orientation, the extension direction of the sample separation unit can correspond to the longitudinal direction of movement, enabling fluid coupling between the second fluid connector and the second fluid interface solely through longitudinal movement. Conversely, this fluid coupling cannot be achieved in a tilted mounting orientation.

[0025] In an embodiment, the mounting device includes a fixing mechanism for securing at least one movable component of the first and second fluid connectors by operating the fixing mechanism. Specifically, the process of mechanically and fluidly coupling the second fluid connector to the second fluid interface may include spatially securing the second fluid connector after sliding. When secured, the second fluid connector is in a specific position and prohibited from movement at a distance from the first fluid connector, the distance corresponding to the length of the implemented sample separation unit. In an embodiment, securing the movable second fluid connector may be a prerequisite for subsequently establishing a sealed connection (particularly a high-pressure resistant connection) between the second fluid interface and the second fluid connector of the sample separation unit.

[0026] In this embodiment, the fixing mechanism includes a fixing rod. Actuating the fixing rod is an intuitive and simple way for the user to secure the corresponding fluid connector to the corresponding fluid interface of the sample separation unit. However, other mechanical solutions for the fixing mechanism are also possible, such as a fixing button that the user can press to secure the corresponding fluid connector. Automatic connection mechanisms are also possible.

[0027] In an embodiment, the retaining rod has a retaining eccentricity or is coupled to a retaining eccentricity configured to secure at least one movable of the first and second fluid connectors by rotating the retaining rod. Highly advantageously, simply pivoting or rotating the retaining rod with the connected retaining eccentricity can trigger a reliable mechanical holding force that secures the respective fluid connector in a user-defined position. Descriptively speaking, when the user actuates the retaining rod, the thicker portion of the retaining eccentricity can press against the fluid connector for securing it.

[0028] In one embodiment, the rotating mechanism includes a hinge. The hinge may be a mechanical bearing connecting two solid objects and may allow only a limited angle of rotation between them along a defined axis of rotation. Therefore, the rotating mechanism allows the user to rotate the first fluid connector only about a predetermined axis of rotation. This avoids erroneous operation of the installation device and thus ensures robust operation in case of failure.

[0029] In a preferred embodiment, the first fluid connector is configured to fluidly couple the sample separation unit to its first fluid interface in a sealed, particularly pressure-resistant manner, by rotating the sample separation unit from a mounting orientation to an alignment orientation. Highly advantageously, the action of shifting the first fluid connector from a mounting orientation to an alignment orientation (where the alignment orientation may correspond to an analytical orientation) can simultaneously and without any further steps establish a fluid-tight seal between the first fluid connector of the mounting device and the first fluid interface of the sample separation unit. Preferably, this sealing configuration is pressure-resistant, i.e., it prevents fluid leakage even when high-pressure fluid flows through the first fluid connector into the first interface of the sample separation unit. For example, the sealing configuration can withstand pressures particularly at least 600 bar, more particularly at least 1200 bar. Therefore, the sealed connection is compatible with the requirements of liquid chromatography, particularly UHPLC.

[0030] Specifically, the first fluid connector may be located downstream of the injector through which the fluid sample to be separated is injected into the mobile phase between the fluid drive unit and the sample separation unit. Optionally, a preheater assembly may be arranged between the injector and the first fluid connector for preheating the fluid sample and / or mobile phase before it reaches the sample separation unit. In another embodiment, the preheater assembly may be integrated into the first fluid connector. Thus, the fluid sample and / or mobile phase may be at or be brought to a high pressure upon reaching or passing through the first fluid connector, the high pressure being provided by the fluid drive unit, such as a piston pump. A significant pressure drop may occur when passing through the sample separation unit, for example, from 1200 bar or higher at the first fluid interface to 30 bar or lower at the second fluid interface. Therefore, high-performance sealing performance may be most advantageous at the first fluid connector.

[0031] In an embodiment, the mounting device includes a first sealing mechanism having a first sealing eccentric member configured to fluidly couple a first fluid connector to a first fluid interface of the sample separation unit in a sealed, particularly high-pressure resistant manner, by rotating the sample separation unit from a mounting orientation to an alignment orientation. Accordingly, the method may include fluidly coupling the first fluid connector to the first fluid interface of the sample separation unit in a sealed, particularly high-pressure resistant manner, by rotating the sample separation unit from a mounting orientation to an alignment orientation. In an embodiment, the mounting device may be configured such that rotating the first fluid connector and the sample separation unit from a mounting orientation to an alignment orientation triggers the formation of a sealed, particularly high-pressure resistant connection between the first fluid interface and the first fluid connector, particularly via the first sealing eccentric member. Therefore, the method may include actuating the first sealing eccentric member by rotating the sample separation unit from a mounting orientation to an alignment orientation, thereby fluidly coupling the first fluid connector to the first fluid interface of the sample separation unit in a sealed, particularly high-pressure resistant manner. Descriptively speaking, for example, the columnar sample separation unit itself can serve as a sealing rod, shifting from an installation orientation to an alignment orientation, thereby acting on the first sealing eccentric to press the first fluid connector together with the first fluid interface of the sample separation unit through its wide portion. Therefore, apart from intuitively rotating the first fluid connector with the sample separation unit from the alignment orientation, no separate action is required for the user to establish a high-pressure connection between the first fluid connector and the sample separation unit. Descriptively speaking, the columnar sample separation unit can also operate as a fixing rod, which only requires tilting to establish a fluid-sealed fluid and mechanical connection. Given the considerable length of the sample separation unit, its rod action can be highly efficient.

[0032] In an embodiment, the second fluid connector is configured to fluidly couple to the second fluid interface of the sample separation unit in a sealed, particularly high-pressure resistant manner. As described above, the second fluid connector can be located downstream of the sample separation unit. Furthermore, the second fluid connector can be located upstream of the detector in the separation section for detecting the fluid sample. Although significant pressure drops may occur along the sample separation unit (e.g., a chromatographic column), the pressure at the second fluid connector can still be significantly higher than ambient pressure, for example, in the range of 20 to 30 bar. To avoid leakage between the second fluid interface and the second fluid connector, it can be advantageous to create a high-pressure resistant seal between the second fluid interface of the sample separation unit and the second fluid connector of the mounting device.

[0033] In one embodiment, the mounting device includes a second sealing mechanism for sealing the second fluid connector to the second fluid interface. This second sealing mechanism can be operated or actuated by the user to establish a fluid-tight connection at the outlet of the sample separation unit.

[0034] In this embodiment, the second sealing mechanism includes a sealing rod. Actuating the sealing rod is an intuitive and simple way for the user to seal the second fluid connector at the second fluid interface of the sample separation unit. However, other mechanical solutions for the sealing mechanism are also possible, such as a sealing button that can be pressed by the user to seal the second fluid connector.

[0035] In one embodiment, the sealing rod has a second sealing eccentric configured to seal the second fluid connector and the second fluid interface by rotating the sealing rod. Advantageously, simply pivoting or rotating the sealing rod with the eccentric element can trigger a fluid sealing force that prevents leakage of fluid (e.g., liquid or gas) at the location between the second fluid interface and the second fluid connector of the sample separation unit. Descriptively speaking, when the user actuates the sealing rod, the thicker portion of the second sealing eccentric can compress the second fluid connector and the second fluid interface of the sample separation unit together.

[0036] In an embodiment, the mounting device is configured such that the second sealing mechanism can only be operated, particularly the sealing rod, after the fixing mechanism has been previously operated, especially after the fixing rod has been previously rotated. For this purpose, the sealing rod and fixing rod can be presented as mechanically coupled dual-rod members. It is highly preferred that the second fluid connector is already in a defined fixed position facing the second fluid interface of the sample separation unit when a fluid-tight or even high-pressure-resistant coupling is established therebetween. To satisfy this condition, the second fluid connector can be first secured by increasing the connection force beyond the fixed level up to the final sealing pressure before a pressure-rigid fluid connection is established between the second fluid connector and the second fluid interface. Therefore, actuation of the sealing rod can be mechanically disabled (e.g., by locking or blocking) until the fixing rod has been actuated to secure the second fluid connector. For example, the sealing rod and fixing rod can be presented as dual rods requiring two user actions. However, the sealing rod and fixing rod can also be embodied as a common mechanism that requires only one user action to complete the fixing and then sealing.

[0037] Preferably, the first fluid connector and the rotating mechanism are longitudinally fixed, while the second fluid connector is capable of longitudinal displacement. This allows for a mechanically simple configuration of the first fluid connector, as it only needs to be configured for rotation, not longitudinal displacement. Correspondingly, the second fluid connector may only need to be configured to move longitudinally, for example, to slide along a track. However, it is not necessary to provide a second fluid connector with rotational functionality, thus maintaining structural simplicity.

[0038] In this embodiment, the mounting device is configured to install the sample separation unit at the sample separation device without tools. Therefore, the user can assemble and disassemble the sample separation unit at the mounting device using only their hands without any tools. Thus, handling the sample separation unit at the mounting device is very simple and can be performed intuitively by the user without requiring specific skills.

[0039] In this embodiment, the alignment orientation corresponds to the orientation of the sample separation unit installed during the separation of the fluid sample by the sample separation unit. Therefore, the alignment orientation can correspond to the analytical orientation or sample separation orientation of the first fluid connector and the sample separation unit mounted thereon.

[0040] In this embodiment, the angle between the mounting orientation and the alignment orientation is no greater than 180°, particularly within the range of 45° to 90°. By allowing rotation angles up to 180°, a sufficiently large lever force can be applied between the first fluid connector and the sample separation unit to establish a fluid-tight connection between them, even if the latter (acting as a sealing lever) is relatively short. However, a rotation angle within the range of 45° to 90° is preferred because this allows the user to start from an anatomically convenient angular position to mount the sample separation unit onto the first fluid connector. However, in other embodiments, rotation angles greater than 180°, such as 270°, can be selected.

[0041] In an embodiment, the sample separation device includes an interface adapter that connects at least one of a first fluid interface and a second fluid interface of the sample separation unit to a corresponding one of a first fluid connector and a second fluid connector of the mounting device. For example, such an interface adapter can be a male-to-female adapter, a female-to-female adapter, or a male-to-male adapter. This type of interface adapter, particularly the one connecting the second fluid interface and the second fluid connector, allows the use of virtually any sample separation unit with a universal mounting device. Typically, different connections between the corresponding fluid connector and the corresponding fluid interface are possible, including tapered-to-taper connections and tapered-forged connections.

[0042] In an embodiment, the method includes rotating (e.g., screwing) the sample separation unit onto a first fluid connector in an installation orientation, thereby mechanically and fluidly coupling the first fluid connector to a first fluid interface. For example, the sample separation unit may first be screwed onto the first fluid connector to form a mechanical connection. By rotating the threaded sample separation unit to an alignment orientation, a fluid-tight connection can be formed between the first fluid connector and the sample separation unit.

[0043] In one embodiment, rotating from mounting orientation to alignment orientation includes rotating the sample separation unit to an upright orientation. Therefore, the sample separation unit can be oriented in a vertical position for sample separation or analysis. However, alternatively, the sample separation unit can also be rotated to a horizontal orientation or even an inclined orientation to separate fluid samples.

[0044] In embodiments, the mounting apparatus may include a preheating device (particularly directly) upstream of the first fluid connector for preheating the fluid sample before it reaches the sample separation unit. Such a preheating unit allows the fluid sample to be brought directly to an elevated (e.g., target) temperature before separation in the sample separation unit, thereby creating defined or favorable separation conditions. If such a preheating device is arranged directly adjacent to the first fluid connector, undesirable cooling between preheating and separation can be avoided or at least strongly suppressed.

[0045] The sample separation unit may be filled with a separation material. This separation material, also known as the stationary phase, can be any material fluid that allows for an adjustable degree of interaction with the sample fluid to enable the separation of different components of the sample fluid. The separation material can be a liquid chromatography column packing material or packing material comprising at least one of the following: polystyrene, zeolite, polyvinyl alcohol, polytetrafluoroethylene, glass, polymer powder, silica, and silica gel, or any of the above materials with chemically modified (coated, capped, etc.) surfaces. However, any packing material with material properties that allow the separation of analytes passing through the material into different components (e.g., due to different types of interactions or affinities between the packing material and the analyte fractions) can be used.

[0046] At least a portion of the sample separation unit may be filled with a fluid separation material, wherein the fluid separation material may comprise beads having a size ranging from substantially 1 μm to substantially 50 μm. Therefore, these beads can be small particles that can fill the separation section of a microfluidic device. The beads may have pores with a size ranging from substantially 0.01 μm to substantially 0.2 μm. The fluid sample can pass through the pores, wherein an interaction can occur between the fluid sample and the pores.

[0047] The sample separation unit can be a chromatographic column used to separate components of a fluid sample. Therefore, exemplary embodiments can be implemented, particularly in the context of a liquid chromatography apparatus.

[0048] The sample separation apparatus can be configured to guide a liquid mobile phase through a sample separation unit. Alternatively, the sample separation apparatus can be used to process a gaseous mobile phase or a mobile phase comprising solid particles. Exemplary embodiments can also be used to process materials that are mixtures of different phases (solid, liquid, and gas). The sample separation apparatus can be configured to conduct the mobile phase through the system under high pressure, particularly at least 600 bar, and more particularly at least 1200 bar.

[0049] This sample separation device can be configured as a microfluidic device. The term "microfluidic device" can specifically refer to a sample separation device as described herein, which allows the delivery of fluid through microchannels having a size order of magnitude less than 500 µm, particularly less than 200 µm, more particularly less than 100 µm, or less than 50 µm or smaller.

[0050] Exemplary embodiments of the sample separation apparatus may be implemented via a sample injector that can draw sample fluid from a fluid container and inject such sample fluid into a conduit for supply to the separation column. During this process, the sample fluid can be compressed from, for example, normal pressure to, for example, several hundred bar or even 1000 bar or higher pressure. An autosampler can automatically inject sample fluid from a vial into the sample loop. The tip or needle of the autosampler may be immersed in the fluid container, drawing fluid into a capillary, and then driven back into the seat, and then injected, for example, towards the sample separation section of the liquid chromatography apparatus via a switchable fluid valve.

[0051] Sample separation equipment can be configured to analyze at least one physical, chemical, and / or biological parameter of at least one component of a sample fluid in a mobile phase. The term "physical parameter" may specifically refer to the size or temperature of the fluid. The term "chemical parameter" may specifically refer to the concentration, affinity, etc., of the analyte fraction. The term "biological parameter" may specifically refer to the concentration of proteins, genes, etc., and the biological activity of the components in a biochemical solution.

[0052] Sample separation equipment can be implemented in various technical environments, such as sensor devices, testing devices, devices for chemical, biological and / or pharmaceutical analysis, capillary electrophoresis devices, liquid chromatography devices, gas chromatography devices, electronic measurement devices, or mass spectrometry devices. In particular, sample separation equipment can be a high-performance liquid chromatography (HPLC) device, which allows for the separation, examination, and analysis of different fractions of analytes.

[0053] Embodiments of the present invention include a sample separation apparatus configured to separate complexes of a sample fluid in a mobile phase. The sample separation apparatus includes a mobile phase driver, such as a pumping system, configured to drive the mobile phase through the sample separation apparatus. A sample separation unit, which can serve as a chromatographic column, is configured to separate the complexes of the sample fluid in the mobile phase. The sample separation apparatus may further include a sample injector configured to introduce the sample fluid into the mobile phase, a detector configured to detect the separated complexes of the sample fluid, a collector configured to collect the separated complexes of the sample fluid, a data processing unit configured to process data received from the fluid separation system, and / or a degassing device for degassing the mobile phase.

[0054] The examples can be implemented in commonly available HPLC systems, such as the analytical Agilent 1290 Infinite II LC system or the Agilent 1290 Infinite II preparative LC / MSD system.

[0055] One embodiment includes a pumping device with a piston for reciprocating within a pump working chamber to compress a liquid in the pump working chamber to a high pressure, under which the compressibility of the liquid becomes significant. Another embodiment includes two pumping devices coupled in series or in parallel.

[0056] The mobile phase (or eluent) can be a pure solvent or a mixture of different solvents. It can be selected, for example, to minimize the retention of the target complex and / or the amount of mobile phase used to run the chromatography. The mobile phase can also be selected to allow for efficient separation of different complexes. The mobile phase may include organic solvents that are often diluted with water, such as methanol or acetonitrile. For gradient runs, water and organics are delivered in separate vials, from which a gradient pump delivers the programmed mixture to the system. Other commonly used solvents may be isopropanol, THF, hexane, ethanol, and / or any combination thereof, or any combination of these with the aforementioned solvents.

[0057] The sample fluid can include any type of process liquid, such as natural samples like juice, bodily fluids like blood plasma, or it can be the result of a reaction such as that from fermentation broth.

[0058] The fluid is preferably a liquid, but may also be a gas and / or a supercritical fluid (as used, for example, in supercritical fluid chromatography – SFC). Attached Figure Description

[0059] Other objects and numerous incidental advantages of the invention will be readily appreciated and better understood by referring to the following more detailed description of embodiments taken in conjunction with the accompanying drawings. Substantially or functionally equivalent or similar features will be referred to by the same reference numerals.

[0060] Figure 1A sample separation apparatus according to an embodiment of the present invention is shown, which is particularly used for high performance liquid chromatography (HPLC).

[0061] Figures 2 to 6 This is an illustration of a mounting device or a portion thereof for mounting a sample separation unit in a sample separation apparatus according to an exemplary embodiment.

[0062] Figure 7 and Figure 8 This is a schematic diagram of a method for installing a sample separation unit in two different states during operation at a sample separation device according to an exemplary embodiment.

[0063] Figure 9 It is using different Figure 7 and Figure 8 Another schematic diagram of a method for installing a sample separation unit at a sample separation device according to another exemplary embodiment in two different states during the operation of another sample separation unit.

[0064] The illustrations in the attached diagram are schematic. Detailed Implementation

[0065] Before referring to the accompanying drawings, exemplary embodiments will be explained in more detail, and some basic considerations will be explained based on exemplary embodiments that have been developed.

[0066] An exemplary embodiment of the present invention provides a mounting device that supports a user during the assembly of a sample separation unit (such as a chromatographic column) into a sample separation apparatus, more specifically, into the column oven of the sample separation apparatus. After a first fluid interface of the sample separation unit is mounted at a first fluid connector of the mounting device in a mounting orientation, the first fluid connector is rotated or pivoted together with the sample separation unit to an alignment orientation in which the sample separation unit remains during subsequent sample separation or analysis.

[0067] For example, the sample separation unit can be mounted on the side wall of the column oven. The sample separation unit can be screwed onto a first fluid connector, which can be presented as an accessory. The columnar sample separation unit can then be used as a lever and manually grasped and rotated by the user to its aligned orientation. This tilting operation can tighten, tension, or tighten an eccentric element (or eccentric member), thereby creating a high-pressure connection between the first fluid connector and the first fluid interface of the sample separation unit. A first capillary can be pre-connected to the first fluid connector, and a second capillary can be pre-connected to the second fluid connector. This eliminates the need for user handling of the capillaries.

[0068] More specifically, an exemplary embodiment of the present invention provides an installation device for fixing and connecting a sample separation unit (e.g., a separation column) to a fluid path, wherein actual sample separation occurs along the fluid path.

[0069] Traditionally, columns used in liquid chromatography are connected to the fluid path via simple wrench-driven fittings (such as forged fittings) or tool-less fittings with quick-connect options. Sometimes, these fittings include a spring to push the capillary forward within the fitting to avoid dead volume (so-called spring-loaded fittings). However, wrench-driven tools are cumbersome to use (e.g., often requiring three hands). Furthermore, dead volume is difficult to control. Such conventional tools are prone to overtightening, often damaging the capillary and fitting. Similarly, ports and / or sample separation units can be damaged or even destroyed by tools that are normally necessary, such as by overtightening fittings. Once the sample separation unit (such as a column for liquid chromatography) is connected to two capillary tubes with the aid of two fittings, the sample separation unit is positioned within the column oven using a clamp-like or other column holder. Manufacturers of chromatography column ovens have developed their own types of column holders. These holders are easily misplaced, and handling requires several additional actions from the user. Often, the column is not securely held within the holder, which is particularly problematic when using more than two columns. This can also impair chromatographic analysis results. Furthermore, the traditional method of installing columns in a liquid chromatography oven may require the user to connect up to 16 capillaries (if the oven has 8 positions) or 4 capillaries (if the oven has 2 positions). This is cumbersome for the user and requires specific skills.

[0070] To overcome at least some of the aforementioned and / or other conventional disadvantages, exemplary embodiments of the present invention provide an installation device for securing a sample separation unit (e.g., a chromatographic column) and freely connecting tools to a fluid path. This installation device can form part of the column oven of a liquid chromatography separation apparatus. By using toolless fittings in the installation device according to exemplary embodiments of the present invention, the user can reliably avoid overtightening, allowing the toolless fittings to have a longer lifespan. Using the installation device according to exemplary embodiments of the present invention reduces the effort and skill required for the user to install or replace sample separation units of a sample separation apparatus. Advantageously, such tasks can be performed with fewer workflow processes. No tools are required, the installation process may involve fewer errors, and it can be performed more quickly. In particular, quick-operating fittings (which may be spring-loaded) can be implemented. When the sample separation unit is connected via the installation device according to exemplary embodiments of the present invention, the dead zone volume can be reduced. Advantageously, the user does not need to worry about the tightness of the connection, because in preferred embodiments, a high-pressure tight or sealed connection can be formed simply by rotating the sample separation unit together with a first fluid connector on which the sample separation unit is pre-assembled. As a result, the risk of error and / or leakage rate can be reduced. No tools are required to operate this installation device.

[0071] Advantageously, the interaction principle between the column insertion and the mounting device according to an exemplary embodiment of the invention can be as follows: the combined mounting device with spring loading for column fixing and column connection to the fluid path allows the user to install the column using only four workflows:

[0072] 1. Connect the sample separation unit to the first fluid connector.

[0073] 2. Move the sample separation unit to the correct orientation or position by rotation. Preferably, but not necessarily, this movement can simultaneously tighten the connection between the sample separation unit and the first fluid connector.

[0074] 3. Position the second fluid connector to fit the length of the sample separation unit (especially the column length), particularly by moving the second fluid connector toward the second fluid interface of the sample separation unit (particularly manually or automatically).

[0075] 4. For example, manually (e.g. by rotating the sealing rod) tighten or fluidly connect the second fluid connector at the sample separation unit so that the sample separation unit is under sufficient pressure for subsequent chromatographic analysis (which may include, for example, 1200 bar or higher pressure).

[0076] Exemplary embodiments of the present invention enable the insertion and fluid coupling of a sample separation unit (e.g., a chromatographic column). When performing the process of inserting the sample separation unit between two (e.g., conical) fluid connectors, the fluid connectors can be slid against each other, for example, on a track. Specifically, one of the (e.g., conical) fluid connectors or couplers can be moved axially along the track and can be fixed at a target position, for example, using a lever mechanism (particularly when the corresponding fluid interface of the sample separation unit has been reached). The other (e.g., conical) fluid connector or coupler can be fixedly arranged on the track but can have a rotation mechanism that allows rotation or turning about a rotation axis. This allows slight rotation of the first fluid connector or coupler to provide a more convenient angle for inserting the sample separation unit into the first fluid connector. Once the sample separation unit is inserted into the first fluid connector or coupler, both can rotate back to the axial position, and the (particularly upper) second fluid connector can move against the (particularly upper) connection point of the sample separation unit, thereby fluidly coupling the sample separation unit from both axial sides. Optionally, but preferably, the position of the upper second fluid connector can be fixed, for example, by using a lever mechanism. An advantageous aspect of exemplary embodiments of this application is the provision of a rotating mechanism for improving usability and the opportunity to establish a sealed, high-pressure, tight coupling solely through the operation of the rotating mechanism. Thus, exemplary embodiments can realize a column coupling mechanism in which one side of the column coupling mechanism can be rotated to simplify column insertion. Therefore, pivoting of one side of the column can be performed to facilitate easier column insertion.

[0077] According to an exemplary embodiment of the present invention, the mechanism for establishing fluid coupling of a chromatographic column can be provided by: (1) inserting the end of the column into a pivot fluid connector (wherein the pivoting mechanism may be attached to a static track), (2) folding the column so that the column is positioned substantially parallel to the track, (3) pushing another fluid connector along the track so that it is fluidly coupled to the end of the column, (4) moving a rod for locking a sliding fluid connector on the track, and (5) moving another eccentrically mounted rod to achieve high-pressure coupling on both sides.

[0078] In one embodiment, a column identification tag reader may be provided for automatic connection. In yet another embodiment, a connection to a solvent preheater may be included. Furthermore, a filter for filtering the fluid may be incorporated into the mounting assembly.

[0079] Now refer to the attached diagram for more details. Figure 1A general schematic diagram of a liquid chromatography separation system is depicted, as an example of a sample separation device 10. A pump, exemplified by a fluid drive unit 20, typically receives the mobile phase from a solvent supply unit 25 via a degasser 27, which degass and thus reduces the amount of gas dissolved in the mobile phase. The fluid drive unit 20—such as a high-pressure pump—drives the mobile phase through a sample separation unit 30 (such as a chromatographic column) that includes a stationary phase. A sampling unit or injector 40 may be disposed between the fluid drive unit 20 and the sample separation unit 30 to inject or add (typically referred to as sample introduction) the sample fluid into the mobile phase. The stationary phase of the sample separation unit 30 is configured to separate complexes of the sample liquid. A detector 50 is provided for detecting the complexes of the separated sample fluid. A fractionation unit 60 may be provided for discharging the complexes of the separated sample fluid.

[0080] While the mobile phase may consist of only one solvent, it can also be a mixture of multiple solvents. This mixing can be low-pressure mixing and positioned upstream of the fluid drive unit 20, such that the fluid drive unit 20 has received and pumped the mixed solvent as the mobile phase. Alternatively, each fluid drive unit 20 may include multiple separate pumping units, each receiving and pumping a different solvent or mixture, such that mixing of the mobile phase (received by the sample separation unit 30) occurs at high pressure and downstream of (or as part of) the fluid drive unit 20. The composition of the mobile phase (mixture) can remain constant over time (so-called isocratic mode) or vary over time (so-called gradient mode).

[0081] A control unit 70, which may be a conventional PC or workstation, can be coupled (as indicated by the dashed arrow) to one or more devices in the sample separation apparatus 10 to receive information and / or control operation. For example, the control unit 70 can control the operation of the fluid drive unit 20 (e.g., set control parameters) and receive information from it about actual operating conditions (e.g., pump outlet, output pressure, flow rate, etc.). The control unit 70 can also control the operation of the solvent supply unit 25 (e.g., set the solvent or solvent mixture to be supplied) and / or the degasser 27 (e.g., set control parameters, such as vacuum level), and can receive information from it about actual operating conditions (e.g., solvent composition supplied over time, flow rate, vacuum level, etc.). The control unit 70 can further control the operation of the sampling unit or injector 40 (e.g., control sample injection or synchronization of sample injection with the operating conditions of the fluid drive unit 20). The sample separation unit 30 can also be controlled by the control unit 70 (e.g., select a specific flow path or column, set the operating temperature, etc.), and—in turn—send information (e.g., operating conditions) to the control unit 70. Accordingly, detector 50 can be controlled by control unit 70 (e.g., regarding spectrum or wavelength settings, setting time constants, starting / stopping data acquisition), and information (e.g., regarding detected sample complexes) can be sent to control unit 70. Control unit 70 can also control the operation of grading unit 60 (e.g., combining data received from detector 50) and provide data back.

[0082] exist Figure 1 As can be seen, the sample separation unit 30 (e.g., a chromatographic column) is arranged together with the preheating device 90 in a temperature control chamber 92 (e.g., a column oven), where a heat source (not shown) can be used for heating. In this way, the sample separation unit 30 can be brought to the target temperature or predetermined temperature required for the sample separation process.

[0083] Figure 1A mounting device 100 according to an exemplary embodiment of the present invention is also schematically shown for assembling and disassembling a sample separation unit 30 in a sample separation apparatus 10. The mounting device 100 is for manual mounting of the sample separation unit 30 onto the sample separation apparatus 10 by a user without the use of tools. For this purpose, the mounting device 100 includes a first fluid connector 102 pre-connected to an inlet capillary 94 and may be presented as a male first fitting. The first fluid connector 102 is configured to be mechanically and fluidly coupled to a first fluid interface 104 (e.g., a female column inlet) of the sample separation unit 30. Furthermore, a second fluid connector 106 (which may be presented as a male second fitting) may be pre-connected to an outlet capillary 96 and may be configured to be mechanically and fluidly coupled to a second fluid interface 108 (e.g., a female column outlet) of the sample separation unit 30. In other embodiments, the sample separation unit 30 may also be implemented having at least one male interface 104 and / or 108. More generally, each fluid interface 104, 108 can provide a fluid connection, particularly but not limited to a female column inlet or outlet. For example... Figure 1 As schematically indicated, the mounting device 100 may further include a rotation mechanism 110, configured for mounting orientation (in) the first fluid interface 104 of the sample separation unit 30 at the first fluid connector 102. Figure 1 (shown in solid lines) and the alignment orientation for aligning the second fluid interface 108 of the mounted sample separation unit 30 with the second fluid connector 106 (in) Figure 1 Rotate the first fluid connector 102 between the two fluid interfaces (shown in dashed lines). When in the alignment orientation, the second fluid interface 108 can then be coupled to the second fluid connector 106 to close the flow path from the fluid drive unit 20 and injector 40 through the temperature control chamber 92 to the detector 50. User-friendly installation of the sample separation unit 30 in an orientation that allows it to face the user during installation is advantageous. Rotating, pivoting, or tilting the sample separation unit 30 into an analytical or sample separation orientation in which subsequent sample separation or analysis will be performed can optionally, but very advantageously, establish a high-pressure robust fluid connection between the first fluid connector 102 and the first fluid interface 104.

[0084] Reference Figures 2 to 6 This section will explain the details regarding the construction of the mounting device 100 according to an exemplary embodiment. (Refer to...) Figures 7 to 9 The method of operating such an installation device 100 according to an exemplary embodiment will be explained in more detail.

[0085] Figures 2 to 6This is a schematic diagram of an installation device 100 for installing a sample separation unit 30 in a sample separation device 10, according to an exemplary embodiment. Figure 2 A three-dimensional view of the mounting device 100 (without the sample separation unit 30) is shown. Figure 3 The mounting device 100 is shown from a top-down view. Figure 4 Details of the slider unit 112 and the corresponding fixing mechanism 114 for moving and fixing the mounting device 100 are shown. Figure 5 This shows details of the rotating mechanism 110 for rotating the first fluid connector 102 of the mounting device 100. Figure 6 Further details of the mounting device 100 are shown, illustrating a double-bar mechanism for securing the second fluid connector 106 and creating a high-pressure seal between the second fluid connector 106 and the second fluid interface 108 of the sample separation unit 30.

[0086] according to Figures 2 to 6 The mounting device 100 is used to install the column sample separation unit 30 (comparative) Figures 7 to 9 Installed in, for example Figure 1 The sample separation equipment shown is 10.

[0087] For this purpose and as a primary reference Figure 2 and Figure 5 The mounting device 100 includes a bottom-side first fluid connector 102, which is a male fitting and configured to connect to a female first fluid interface 104 at the inlet of the sample separation unit 30 (compare). Figure 7 Mechanical and fluid coupling. As shown in the figure, the first fluid connector 102 is a tapered fluid connector for insertion into a correspondingly shaped recess in the first fluid interface 104 of the sample separation unit 30.

[0088] As in Figure 2 , Figure 4 and Figure 6 As best shown, the top-side second fluid connector 106, presented as another male accessory, is configured to connect with the second fluid interface 108 (compare) at the outlet of the sample separation unit 30. Figure 7 Mechanical and fluid coupling. In the illustrated embodiment, the second fluid connector 106 is a tapered fluid connector for insertion into a recess of a corresponding shape and size in the second fluid interface 108 of the sample separation unit 30.

[0089] In addition, it is set in Figure 2 and Figure 5 The rotating mechanism 110 is shown in the image. (As shown in the image...) Figure 2 and Figure 5As shown, the rotating mechanism 110 includes a hinge 122. The rotating mechanism 110 is configured to rotate the first fluid connector 102 between tilted mounting orientations for mounting the first fluid interface 104 of the sample separation unit 30 at the first fluid connector 102 in a user-friendly manner. The tilted mounting orientation is not in... Figures 2 to 6 As shown in the text, but Figure 7 As shown in the figure. Furthermore, the user can actuate the rotation mechanism 110 to shift the first fluid connector 102 to a vertical alignment orientation, as shown, for aligning the second fluid interface 108 of the mounted sample separation unit 30 with the second fluid connector 106. When in the alignment orientation, the mounting device 100, together with the mounted sample separation unit 30, is prepared for subsequently coupling the second fluid interface 108 to the second fluid connector 106 by sliding the second fluid connector 106 downwards until the second fluid connector 106 abuts against the second fluid interface 108 of the sample separation unit 30. In the vertical alignment orientation (see...), Figure 2 and Figure 8 In the sample separation unit 30, the first fluid connector 102 and the sample separation unit 30 are both in their final orientation and position for sample separation or analysis.

[0090] like Figure 2 and Figure 4 As indicated by the double arrows, the second fluid connector 106 is configured to move longitudinally toward but not reach the first fluid connector 102 in a vertical direction to align and couple the second fluid interface 108 of the sample separation unit 30 with the second fluid connector 106. However, in an inclined mounting orientation, such mechanical and fluid coupling is not possible. In the illustrated embodiment, the first fluid connector 106 cannot move longitudinally and is therefore longitudinally fixed. Descriptively speaking, the first fluid connector 102 is rotatable but cannot move longitudinally, while the second fluid connector 106 is not rotatable but can move longitudinally. Therefore, the first fluid connector 102 and the rotating mechanism 110 are longitudinally fixed, while the second fluid connector 106 is longitudinally movable. More specifically, the movable second fluid connector 106 is connected to a slider unit 112, which is mounted on the support 156 (e.g., as a support according to the...). Figure 1The mounting device 100 is mounted on the vertical wall of the column furnace in the embodiment of the temperature control chamber 92, so as to be able to slide along the track 118 in the vertical longitudinal sliding direction together with the second fluid connector 106. When the alignment orientation is parallel to the longitudinal sliding direction, the mounting orientation is tilted relative to the longitudinal sliding direction. By sliding the second fluid connector 106 along the track 118 (presented here as a continuous track) to the desired vertical position via the sliding unit 112, the mounting device 100 can be adjusted to sample separation units 30 of different lengths. Therefore, the universal mounting device 100 can be used compatiblely with the sample separation unit 30 over a large length range (e.g., from 80 mm to 300 mm).

[0091] Now for reference Figure 2 , Figure 3 , Figure 4 and Figure 6 The mounting device 100 includes a fixing mechanism 114 for securing the longitudinally movable second fluid connector 106 in a defined vertical position after its vertical position has been adjusted by sliding the slider unit 112 vertically along the track 118. Fixing can be achieved by operating the fixing mechanism 114. For this purpose, the fixing mechanism 114 includes a fixing rod 116 that can be manually operated by the user. Figure 4 As best shown, the retaining rod 116 has an eccentric member 120, which is functionally connected to the second fluid connector 106 and configured to secure the second fluid connector 106 by rotating the retaining rod 116.

[0092] Advantageously, the first fluid connector 102 is configured to fluidly couple with the first fluid interface 104 of the sample separation unit 30 in a sealed and high-pressure resistant manner when the sample separation unit 30 on the first fluid connector 102 is rotated from the mounting orientation to the alignment orientation. For this purpose, the mounting device 100 may be equipped with a first sealing mechanism 152 having an eccentric member 134. The eccentric member 134 may be configured to rotate together with the mounted sample separation unit 30 from the mounting orientation to the alignment orientation. Thus, the first fluid connector 102 fluidly couples with the first fluid interface 104 of the sample separation unit 30 in a sealed and high-pressure resistant manner. Therefore, even at high pressure values, such as 1200 bar or higher, leakage at the fluid connection between the first fluid interface 104 of the sample separation unit 30 and the first fluid connector 102 can be reliably prevented. Advantageously, when the cylindrical sample separation unit 30 is mounted on the first fluid connector 102, a high-pressure resistant seal can be generated simply by rotating the cylindrical sample separation unit 30 from the mounting orientation to the alignment orientation. During this rotation, considering the considerable length of the sample suppression unit 30 and its therefore high leverage force, the sample separation unit 30 acts as a highly efficient sealing lever. In other words, rotating the first fluid connector 102 together with the sample separation unit 30 from its mounting orientation to its alignment orientation triggers a sealed and high-pressure resistant connection between the first fluid interface 104 and the first fluid connector 102 via an eccentric element 134 cooperating with the sample separation unit 30. No additional hardware is required to establish a high-pressure resistant sealing connection on the bottom side. Due to the... Figure 1 The first fluid connector 102 is fluidly connected to the fluid drive unit 20 and the ejector 40, and is therefore exposed to the high pressure provided by the fluid drive unit 20. Therefore, a reliable high-pressure tight seal at the transition between the first fluid connector 102 and the first fluid interface 104 of the sample separation unit 30 is most advantageous.

[0093] Referring now to the top side, the second fluid connector 106 can also be configured to fluidly couple to the second fluid interface 108 of the sample separation unit 30 in a sealed and preferably high-pressure resistant manner. The top-side second fluid connector 106 can be connected to... Figure 1 The detector 50. Although the main portion of the high pressure generated by the fluid drive unit 20 decreases along the sample separation unit 30, an overpressure of, for example, 20 bar to 30 bar may still exist at the second fluid connector 106. To address this overpressure, a second sealing mechanism 124 can be provided to seal the second fluid connector 106 to the second fluid interface 108 even in the event of significant overpressure at this transition point. Figure 2 , Figure 3 and Figure 6As shown, the second sealing mechanism 124 includes a sealing rod 126 that can be manually operated by a user. The sealing rod 126 has an eccentric element 132 (comparative). Figure 6 The eccentric member 132 is configured to seal the second fluid connector 106 and the second fluid interface 108 by rotating the sealing rod 126. Rotating the sealing rod 126 can put the eccentric member 132 under tension and can seal the second fluid connector 106 and the second fluid interface 108 of the sample separation unit 30 together.

[0094] However, it may be desirable to achieve a seal between the second fluid connector 106 and the second fluid interface 108 only after the second fluid connector 106 has been secured in the desired vertical position. To ensure compliance with this preferred sequence, the sealing rod 126 and the retaining rod 116 are mechanically and functionally coupled. More specifically, the sealing rod 126 and the retaining rod 116 are coupled such that the sealing rod 126 can only be rotated after the retaining rod 116 has been previously rotated. Otherwise, the sealing rod 126 is mechanically blocked by the retaining rod 116.

[0095] As described above, the entire installation device 100 is configured so that the sample separation unit 30 can be installed on the sample separation device 10 manually by the user without the use of any tools.

[0096] Reference Figures 2 to 6 Any eccentric mechanism described (see reference numerals 120, 132, 134) may optionally, but advantageously, be biased by a biasing element (e.g., a spring or magnet) to further strengthen the resulting connection. By taking this measure, the corresponding lever mechanism can be operated with an additional biasing force.

[0097] Figure 7 and Figure 8 This is a schematic diagram of a method for installing the sample separation unit 30 in two different states during operation at the sample separation device 10 according to an exemplary embodiment. Figure 7 As shown, the first fluid connector 102 can be directly arranged downstream of the optional preheater device 90, which in turn is arranged downstream of the ejector 40 and the fluid drive unit 20 (compare). Figure 1 Therefore, during sample separation, the first fluid connector 102 is under a high pressure, for example, 1200 bar. Most of this high pressure decreases as the mobile phase and fluid sample flow through the sample separation unit 30. At the second fluid connector 106, the pressure is typically between 20 and 30 bar during operation of the sample separation device 10. Downstream of the second fluid connector 106, the separated fluid sample can be detected in the detector 50 (for comparison). Figure 1 (In the following text, reference will be made to...) Figure 7 and Figure 8Explain the process of mounting the sample separation unit 30 on the mounting device 100 to establish a quick connection.

[0098] Figure 7 The diagram shows a rotating mechanism 110, a first fluid connector 102, and a first fluid interface 104 of a sample separation unit 30 (e.g., a chromatographic column) in an orientation suitable for installation. With the first fluid connector 102 in its orientation, the user can mount the first fluid interface 104 of the sample separation unit 30 onto the first fluid connector 102. This... Figure 7 The reference numeral "1" is used to indicate this. According to this process, the first fluid connector 102 is mechanically and fluidly coupled to the first fluid interface 104 of the sample separation unit 30, but may not be pressure-sealed. For example, the process may involve hand-tightening the column onto the first fluid connector 102. More generally, the user can rotate the sample separation unit 30 onto the first fluid connector 102 during installation orientation, thereby mechanically and fluidly coupling the first fluid connector 102 to the first fluid interface 104. For the user to perform this task, it is convenient to orient the sample separation unit 30 in an inclined manner.

[0099] like Figure 7 As indicated by reference numeral "2" in the accompanying drawings, the process may subsequently include transferring the rotating mechanism 110, the first fluid connector 102, and the sample separation unit 30 from the sample according to... Figure 7 The mounting orientation is rotated to an upright alignment orientation to align the second fluid interface 108 of the sample separation unit 30 with the second fluid connector 106 of the mounting device 100. Therefore, rotating from the mounting orientation to the alignment orientation can include rotating the sample separation unit 30 to an upright or vertical orientation. The result of this process is as follows: Figure 8 As shown. Through this rotation process, the column-type sample separation unit 30 can be locked simultaneously and the connection can be secured by snapping the column in. In this process, the column acts as a lever. For example, the angle β between the installation orientation and the alignment orientation can be 45°. In the alignment orientation, which can also be called the analytical orientation, the sample separation unit 30 is already in the position and orientation for sample separation or analysis. Therefore, the alignment orientation corresponds to the orientation of the sample separation unit 30 installed during the separation of fluid samples by the sample separation unit 30.

[0100] Advantageously, the process of rotating the rotating mechanism 110, the first fluid connector 102, and the sample separation unit 30 from the mounting orientation to the alignment orientation triggers a simultaneously sealed and pressure-tight connection between the first fluid interface 104 and the first fluid connector 102 without any additional action or measure. This can be achieved, for example, by an eccentric mechanism. For example, this can be accomplished by actuating the eccentric element 134 by rotating the sample separation unit 30 from the mounting orientation to the alignment orientation, thereby fluidly coupling the first fluid connector 102 to the first fluid interface 104 of the sample separation unit 30 in a sealed and pressure-resistant manner (as referenced above). Figures 2 to 6 (as described above). Therefore, according to the exemplary embodiments described in this invention, creating a high-pressure sealed connection at the inlet side of the sample separation unit 30 becomes a simple, quick, and fault-resistant task. There is no need for such operating tools, nor is it necessary to handle minute capillary connections or perform delicate tasks.

[0101] Now for reference Figure 8 When the rotating mechanism 110, the first fluid connector 102 and the sample separation unit 30 have been transferred under alignment and orientation, the second fluid connector 106 can be mechanically and fluidly coupled to the second fluid interface 108 of the sample separation unit 30.

[0102] As indicated by reference numeral "3" in the accompanying drawings, the mechanical and fluid coupling of the second fluid connector 106 to the second fluid interface 108 in alignment and orientation involves sliding the second fluid connector 106 onto the second fluid interface 108. This can be achieved by downwardly placing, driving, or guiding the slider unit 112 until the second fluid connector 106 reaches the second fluid interface 108 of the sample separation unit 30. For example, the slider unit 112 can engage with a vertical track 118 (which is presented herein as a stepping track).

[0103] In addition, such as Figure 8 As indicated by reference numeral "4" in the accompanying drawings, mechanically and fluidly coupling the second fluid connector 106 to the second fluid interface 108 may include spatially fixing the second fluid connector 106 by rotating it about the fixing rod 124 of the fixing mechanism 114 after sliding. Finally, mechanically and fluidly coupling the second fluid connector 106 to the second fluid interface 108 may include sealing the second fluid connector 106 at the second fluid interface 108 by rotating the sealing rod 126 of the second sealing mechanism 124 after fixing. As shown in the figure, it can be based on Figure 8 Establish quick connections to cone-within-cone geometry.

[0104] Figure 9 It is based on the use of different Figure 7 and Figure 8Another exemplary embodiment of another sample separation unit 30, and another schematic diagram of a method for installing the sample separation unit 30 at the sample separation device 10.

[0105] according to Figure 9 Implementation examples and according to Figure 7 and Figure 8 The difference in the embodiments is particularly that, according to Figure 9 An interface adapter 150 is provided, which is arranged between the second fluid interface 108 of the sample separation unit 30 and the second fluid connector 106 of the mounting device 100. More specifically, the interface adapter 150 connects the second fluid interface 108 of the sample separation unit 30 to the second fluid connector 106 of the mounting device 100, for example, when the second fluid interface 108 and the second fluid connector 106 cannot be directly connected due to shape and / or size mismatch. In the illustrated embodiment, the second fluid connector 106 is a male connector with a tapered protrusion assembly. The second fluid interface 108 may have a shape incompatible with the tapered protrusion assembly, for example, because the second fluid interface 108 is male or female, its shape and / or size may not match the tapered protrusion assembly of the second fluid connector 106. When the interface adapter 150 is inserted between the second fluid interface 108 and the second fluid connector 106, the interface adapter 150 can make the latter two components compatible. Specifically, the interface adapter 150 may have a first mechanical and fluid side that mates with the second fluid connector 106, and a second mechanical and fluid side that mates with the second fluid interface 108. By providing a suitable interface adapter 150, the mounting device 100 can become more versatile by supporting different column types or other sample separation units 30. For example, if the sample separation unit 30 is a chromatographic separation column that does not support cone-in-cone fittings, then using the interface adapter 150 may be advantageous.

[0106] It should be noted that the term "comprising" does not exclude other elements, and "a" or "an" does not exclude multiple elements. Similarly, elements described with respect to different embodiments can be combined. It should also be noted that the reference numerals in the claims should not be construed as limiting the scope of the claims.

Claims

1. A mounting device (100) for mounting a sample separation unit (30), said sample separation unit (30) being configured to separate complexes in a fluid sample, wherein, The mounting device (100) includes: A first fluid connector (102) is configured to be mechanically and fluidly coupled to a first fluid interface (104) of the sample separation unit (30). The second fluid connector (106), configured to be mechanically and fluidly coupled to the second fluid interface (108) of the sample separation unit (30), and A rotating mechanism (110) is configured to rotate the first fluid connector (102) between mounting orientation and alignment orientation. The installation orientation configuration involves mounting the first fluid interface (104) of the sample separation unit (30) at the first fluid connector (102), and The alignment orientation configuration is to align the second fluid interface (108) of the sample separation unit (30) mounted at the first fluid connector (102) with the second fluid connector (106), and then couple the second fluid interface (108) with the second fluid connector (106).

2. The mounting device (100) according to claim 1, wherein, The first fluid connector (102) is a male fluid connector, and / or the second fluid connector (106) is a male fluid connector.

3. The mounting device (100) according to claim 1 or 2, wherein, At least one of the first fluid connector (102) and the second fluid connector (106) is configured to move toward the other fluid connector (106, 102) respectively to couple the second fluid interface (108) of the sample separation unit (30) to the second fluid connector (106) in the alignment orientation.

4. The mounting device (100) according to claim 3, wherein, At least one of the first fluid connector (102) and the second fluid connector (106) is connected to the longitudinal guide unit (112) to be able to move in a guided manner along the longitudinal direction of motion.

5. The mounting device (100) according to claim 4, wherein, The alignment orientation is parallel to the longitudinal direction of movement.

6. The mounting device (100) according to claim 4, wherein, The installation orientation is tilted relative to the longitudinal direction of movement.

7. The mounting device (100) according to claim 3, comprising a fixing mechanism (114) for fixing at least one movable of the first fluid connector (102) and the second fluid connector (106) by operating the fixing mechanism (114).

8. The mounting device (100) according to claim 7, wherein, The fixing mechanism (114) includes at least one of the group consisting of a fixing rod (116), a fixing button, and an automatic fixing mechanism.

9. The mounting device (100) according to claim 8, wherein, The fixing rod (116) has an eccentric element (120) configured to secure at least one of the first fluid connector (102) and the second fluid connector (106) by rotating the fixing rod (116).

10. The mounting device (100) according to claim 1, wherein, The rotating mechanism (110) includes a hinge (122).

11. The mounting device (100) according to claim 1, wherein, The first fluid connector (102) is configured to fluidly couple to the first fluid interface (104) of the sample separation unit (30) in a sealed manner by rotating the sample separation unit (30) from the mounting orientation to the alignment orientation.

12. The mounting device (100) according to claim 11, comprising a first sealing mechanism (152) having an eccentric element (134) configured to fluidly couple the first fluid connector (102) to the first fluid interface (104) of the sample separation unit (30) in a sealing manner by rotating the sample separation unit (30) from the mounting orientation to the alignment orientation.

13. The mounting device (100) according to claim 1, wherein, The second fluid connector (106) is configured to fluidly couple to the second fluid interface (108) of the sample separation unit (30) in a sealed manner.

14. The mounting device (100) according to claim 13, comprising a second sealing mechanism (124) for sealing the second fluid connector (106) to the second fluid interface (108), wherein, The second sealing mechanism (124) includes a sealing rod (126), wherein the sealing rod (126) has an eccentric element (132) configured to seal the second fluid connector (106) to the second fluid interface (108) by rotating the sealing rod (126).

15. The mounting device (100) according to claim 14, comprising a fixing mechanism (114) for fixing at least one movable of the first fluid connector (102) and the second fluid connector (106) by operating the fixing mechanism (114); The mounting device (100) is configured such that the second sealing mechanism (124) can only be operated after the fixing mechanism (114) has been previously operated.

16. The mounting device (100) according to claim 1, comprising at least one of the following features: in, The first fluid connector (102) and the rotating mechanism (110) are longitudinally fixed, and the second fluid connector (106) is capable of longitudinal displacement; The configuration is such that rotating the first fluid connector (102) and the sample separation unit (30) from the mounting orientation to the alignment orientation triggers the formation of a sealed connection between the first fluid interface (104) and the first fluid connector (102); The sample separation unit (30) is configured to be installed without tools at the sample separation device (10); Wherein, the alignment orientation corresponds to the orientation of the sample separation unit (30) installed during the separation of the fluid sample by the sample separation unit (30); Wherein, the angle (β) between the installation orientation and the alignment orientation is not greater than 180°; The installation orientation is tilted relative to the alignment orientation.

17. A sample separation device (10) for separating fluid samples, wherein, The sample separation device (10) includes: A fluid drive unit (20) is configured to drive a mobile phase and the fluid sample injected into the mobile phase; Sample separation unit (30), configured to separate the fluid sample; and The mounting device (100) according to any one of claims 1 to 16 is used to mount the sample separation unit (30) at the sample separation device (10).

18. The sample separation apparatus (10) according to claim 17, further comprising at least one of the following features: The sample separation device (10) includes an interface adapter (150) that connects at least one of the first fluid interface (104) and the second fluid interface (108) of the sample separation unit (30) on one side to a corresponding one of the first fluid connector (102) and the second fluid connector (106) of the mounting device (100) on the other side. Temperature control chamber (92), in which at least a portion of the sample separation unit (30) and the mounting device (100) are arranged; Includes a preheating device (90) for preheating the fluid sample and / or mobile phase upstream of the sample separation unit (30); The sample separation device (10) is configured as a chromatographic sample separation device; The sample separation unit (30) is a chromatographic separation column; Detector (50), configured to detect the separated fluid sample; A grading unit (60) configured to collect the separated fractions of the fluid sample; A control unit (70) configured to control the operation of the sample separation device (10); Degassing device (27) for degassing at least a portion of the mobile phase.

19. A method of installing a sample separation unit (30), said sample separation unit being configured to separate complexes in a fluid sample, wherein, The method includes: The first fluid interface (104) of the sample separation unit (30) is mounted in an installation orientation at the first fluid connector (102) to mechanically and fluidly couple the first fluid connector (102) to the first fluid interface (104) of the sample separation unit (30); Then, the rotating mechanism (110) is rotated from the mounting orientation to the alignment orientation so that the second fluid interface (108) of the sample separation unit (30) mounted at the first fluid connector (102) is aligned with the second fluid connector (106); and In the alignment and orientation, the second fluid connector (106) is mechanically and fluidly coupled to the second fluid interface (108) of the sample separation unit (30).

20. The method of claim 19, comprising at least one of the following features: in, The method includes rotating the sample separation unit (30) onto the first fluid connector (102) in the installation orientation, thereby mechanically and fluidly coupling the first fluid connector (102) to the first fluid interface (104); The rotation from the mounting orientation to the alignment orientation includes rotating the sample separation unit (30) from an inclined orientation to an upright orientation; The sample separation unit (30) is rotated from the mounting orientation to the alignment orientation to trigger a sealed connection between the first fluid interface (104) and the first fluid connector (102); The mechanical and fluid coupling of the second fluid connector (106) to the second fluid interface (108) in the alignment and orientation includes sliding the second fluid connector (106) onto the second fluid interface (108), wherein the mechanical and fluid coupling of the second fluid connector (106) to the second fluid interface (108) includes spatially fixing the second fluid connector (106) after the sliding, and wherein the mechanical and fluid coupling of the second fluid connector (106) to the second fluid interface (108) includes sealing the second fluid connector (106) at the second fluid interface (108) after the fixing. The method includes fluidly coupling the first fluid connector (102) to the first fluid interface (104) of the sample separation unit (30) in a sealed manner by rotating the sample separation unit (30) from the mounting orientation to the alignment orientation, wherein the method includes actuating the eccentric member (134) by rotating the sample separation unit (30) from the mounting orientation to the alignment orientation, thereby fluidly coupling the first fluid connector (102) to the first fluid interface (104) of the sample separation unit (30) in a sealed manner.

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