A fluorescence detection system for a biochip

Abstract

The present invention discloses a fluorescence detection system for a biochip, which includes a bracket and an imaging acquisition component vertically arranged on the bracket. A first filter is arranged below the imaging acquisition component; a light source component is arranged below the first filter. The light source component includes a light source turntable, the light source turntable is coaxial with the imaging acquisition component, and a white light source is arranged at the center of the light source turntable; a number of pairs of monochromatic light sources are arranged on the light source turntable. Each pair of monochromatic light sources is symmetric about the center of the light source turntable. The light-emitting end of the monochromatic light source points to directly above the white light source. A second filter is arranged at the light-emitting end of the monochromatic light source, and a light homogenizing component is arranged between the monochromatic light source and the second filter. The advantages of the present invention are as follows: It can simultaneously meet detection indexes such as high optical path uniformity, large detection field of view, and high resolution.

Description

Technical Field

[0001] The present invention relates to the field of fluorescence detection, and particularly to a fluorescence detection system for a biochip. Background Art

[0002] In recent years, single-molecule detection technologies represented by digital PCR and digital ELISA have developed rapidly. The change in the target detection dimension has put forward higher requirements for the performance of the fluorescence detection system (such as: higher optical path uniformity, larger detection field of view (see, for example, Wang Zicheng et al., Design of a large field of view fluorescence microscopy detection system applied to dPCR, optical design, 2021, Vol. 41, No. 1: 6), higher resolution, etc.). In the prior art, for example, the Chinese patent application with publication number CN112345503A discloses a multiplex fluorescence detection device for aligning a sample, including: a lens located directly above the sample and aligned with the sample; an annular illumination device sleeved around the lens and having a plurality of light-emitting points distributed annularly; a plurality of laser light sources respectively emitting lasers; optical fibers connecting the respective light-emitting points of the annular illumination device and the respective laser light sources; a camera located directly above the lens and aligned with the lens for collecting fluorescence signals; a filter wheel horizontally arranged between the camera and the lens and annularly arrayed with a plurality of filter plates corresponding to the emission wavelengths of different fluorescent substances; and a motor for driving the filter wheel to rotate so that a filter plate is located directly above the lens. However, at present, the fluorescence detection systems adopted in most single-molecule detection devices cannot simultaneously meet the detection indexes such as high optical path uniformity, large detection field of view, and high resolution. Any shortcoming will directly affect the detection sensitivity and quantitative accuracy of nucleic acid and protein single molecules, greatly restricting the development and application of single-molecule detection technologies. Therefore, it is of great significance to develop a new fluorescence detection system. Summary of the Invention

[0003] The technical problem to be solved by the present invention is as follows:

[0004] The technical problem that the fluorescence detection systems adopted in single-molecule detection devices in the prior art cannot simultaneously meet the detection indexes such as high optical path uniformity, large detection field of view, and high resolution.

[0005] The present invention solves the above technical problem by the following technical means:

[0006] A fluorescence detection system for a biochip, including a bracket and an imaging acquisition component vertically arranged on the bracket, and a first filter is arranged below the imaging acquisition component;

[0007] A light source component is arranged below the first filter, the light source component includes a light source turntable, the light source turntable is coaxial with the imaging acquisition component, and a white light source is arranged at the center of the light source turntable;

[0008] A number of pairs of monochromatic light sources are provided on the light source turntable. Each pair of monochromatic light sources is symmetric about the center of the light source turntable. The light-emitting end of the monochromatic light source points directly above the white light source. A second filter is provided at the light-emitting end of the monochromatic light source, and a light homogenizing component is provided between the monochromatic light source and the second filter.

[0009] When the fluorescence detection system for a biochip in the present invention is actually applied, the imaging acquisition component is used for imaging the sample to be detected and collecting fluorescence signals. The first filter can filter out the emitted fluorescence in non-characteristic bands and separate the emitted fluorescence in characteristic bands; the white light source can provide a bright-field environment for focusing and imaging the sample to be detected; the monochromatic light source can excite the fluorescent substances in the sample to be detected to generate fluorescence signals that can be detected by optical devices, and the light homogenizing component can enhance the uniformity of the light signal and improve the light energy utilization rate. The fluorescence detection system for a biochip provided by the present invention has a compact structure, greatly reducing the space occupied by the hardware, providing favorable conditions for the design and development of portable and miniaturized instruments; the coaxial cross-oblique shooting scheme of the imaging acquisition component, the white light source, and the monochromatic light source proposed by the present invention can effectively reduce the energy loss during the transmission of the light signal, which is beneficial to the detection of weak fluorescence signals in the sample to be detected. The use of the white light source illumination-assisted imaging acquisition component focusing and imaging scheme can provide favorable conditions for the high-precision capture of fluorescence signals in the sample to be detected. The system can simultaneously meet detection indexes such as high optical path uniformity, large detection field of view, and high resolution. Based on this system, the effective detection of fluorescence signals in nucleic acid or protein amplification biochips can be realized.

[0010] Optimally, it further includes a filter turntable rotatably mounted on the bracket. A filter turntable driving mechanism capable of driving the filter turntable to rotate is provided on the bracket. A number of first filters are provided, and all the first filters are distributed on the filter turntable in a circular array. The filter turntable driving mechanism can drive the filter turntable to drive the first filter to rotate below the imaging acquisition component;

[0011] The filter turntable driving mechanism adopts a motor, the motor shaft is vertically downward, and the filter turntable is mounted on the motor shaft.

[0012] During actual application, the motor drives the filter turntable to rotate, thereby driving different first filters to rotate below the imaging acquisition component, which can meet the multi-channel detection requirements.

[0013] Optimally, the light source assembly further includes a light source turntable driving mechanism, and the light source turntable driving mechanism can drive the light source turntable to rotate;

[0014] The light source turntable driving mechanism adopts a motor, the motor shaft is vertically upward and coaxial with the imaging acquisition component, and the light source turntable is mounted on the motor shaft.

[0015] Optimized, a number of pairs of fixing seats are provided on the light source turntable. Each pair of fixing seats is symmetric about the center of the light source turntable. The inside of the fixing seat is axially penetrated. The fixing seat is inclined. The monochromatic light source is arranged at the lower end of the fixing seat. The second filter is arranged at the upper end of the fixing seat. The light homogenizing component is arranged inside the fixing seat.

[0016] Optimized, the light homogenizing component includes an aspherical lens, a fly-eye lens, and a focusing lens arranged in sequence along the light output direction of the monochromatic light source.

[0017] The light homogenizing component formed by the aspherical lens, the fly-eye lens, and the focusing lens can effectively enhance the uniformity of the optical signal and improve the utilization rate of light energy.

[0018] Optimized, a lower fixing seat is arranged at the lower end of the fixing seat. The monochromatic light source and the aspherical lens are arranged on the lower fixing seat.

[0019] Optimized, a light-transmitting hole coaxial with the fixing seat is arranged in the lower fixing seat. The light-transmitting hole is located between the monochromatic light source and the aspherical lens.

[0020] Optimized, a pressing ring is arranged inside the upper end of the lower fixing seat. The aspherical lens is pressed in the lower fixing seat by the pressing ring.

[0021] Optimized, a heat dissipation component is arranged at the bottom of the lower fixing seat;

[0022] The heat dissipation component includes a number of parallel heat dissipation fins.

[0023] Optimized, a mounting seat is arranged at the upper end of the fixing seat. The fly-eye lens is pressed inside the fixing seat by the mounting seat. A pressing ring is arranged inside the upper end of the mounting seat. The focusing lens and the second filter are pressed inside the mounting seat by the pressing ring.

[0024] The advantages of the present invention are as follows:

[0025] 1. When the fluorescence detection system for biochips in the present invention is actually applied, the imaging acquisition component is used for imaging the sample to be detected and collecting the fluorescence signal. The first filter can filter the emitted fluorescence in non-characteristic wavelength bands and separate the emitted fluorescence in characteristic wavelength bands. The white light source can provide a bright field environment for focusing and imaging the sample to be detected. The monochromatic light source can excite the fluorescent substances in the sample to be detected to generate fluorescence signals that can be detected by optical devices. The light homogenizing component can enhance the uniformity of the light signal and improve the utilization rate of light energy. The fluorescence detection system for biochips provided by the present invention has a compact structure, greatly reducing the space occupied by the hardware, providing favorable conditions for the design and development of portable and miniaturized instruments. The coaxial cross-oblique shooting scheme of the imaging acquisition component, the white light source, and the monochromatic light source proposed by the present invention can effectively reduce the energy loss during the transmission of the light signal, which is beneficial to the detection of weak fluorescence signals in the sample to be detected. The use of the white light source illumination-assisted imaging acquisition component focusing and imaging scheme can provide favorable conditions for the high-precision capture of fluorescence signals in the sample to be detected. This system can simultaneously meet detection indexes such as high optical path uniformity, large detection field of view, and high resolution. Based on this system, the effective detection of fluorescence signals in nucleic acid or protein amplification biochips can be realized.

[0026] 2. During actual application, the motor drives the filter turntable to rotate, thereby driving different first filters to rotate below the imaging acquisition component, which can meet the multi-channel detection requirements.

[0027] 3. The light homogenizing component formed by the aspherical lens, the fly-eye lens, and the focusing lens can effectively enhance the uniformity of the light signal and improve the utilization rate of light energy. Description of the Drawings

[0028] Figure 1 、 2 is a perspective view (hiding the bracket) of a fluorescence detection system for biochips from different perspectives in an embodiment of the present invention;

[0029] Figure 3 is the front view of a fluorescence detection system for biochips in an embodiment of the present invention;

[0030] Figure 4 is the right view of a fluorescence detection system for biochips in an embodiment of the present invention;

[0031] Figure 5 is Figure 4 the sectional view taken along A-A in

[0032] Figure 6 is the perspective view of the monochromatic light source in an embodiment of the present invention;

[0033] Figure 7 is the front view of the monochromatic light source in an embodiment of the present invention;

[0034] Figure 8 For Figure 7 sectional view B-B in the middle;

[0035] Figure 9 、 10 is an exploded view of a fluorescence detection system for a biochip in an embodiment of the present invention; wherein,

[0036] Bracket - 1;

[0037] Imaging acquisition component - 2; Camera - 21; Telecentric lens - 22;

[0038] First filter - 3; Filter turntable - 31; Filter turntable drive mechanism - 32;

[0039] Light source component - 4; Mounting base - 40; Light source turntable - 41; White light source - 42; Monochromatic light source - 43; Second filter - 44; Light homogenizing component - 45; Light source turntable drive mechanism - 46; Fixed seat - 47; Lower fixed seat - 48; Heat dissipation component - 49; Upper retaining ring - 401; Aspherical lens - 451; Fly's eye lens - 452; Focusing lens - 453; Light transmission hole - 481; Lower retaining ring - 482. Specific embodiments

[0040] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts fall within the scope of protection of the present invention.

[0041] With comprehensive reference to Figure 1-3 , a fluorescence detection system for a biochip includes a bracket 1, an imaging acquisition component 2, a first filter 3, and a light source component 4.

[0042] In this embodiment, the main function of the bracket 1 is to provide an installation position for the remaining components. The bracket 1 is not limited to a specific shape, as long as it can meet the requirements of installing and cooperating with each component and realizing the corresponding functions. As Figure 3 shown, the camera 21 is installed on the bracket 1 through a mounting base, the filter turntable drive mechanism 32 is installed on the bracket 1 through a mounting base, and the light source turntable drive mechanism 46 is directly installed on the bracket 1.

[0043] As Figure 3 shown, the imaging acquisition component 2 is vertically arranged on the bracket 1. As Figure 5 shown, a first filter 3 is arranged below the imaging acquisition component 2; AsFigure 4 , 5 As shown in 5 , a light source assembly 4 is disposed below the first filter 3. The light source assembly 4 includes a light source turntable 41, which is coaxial with the imaging acquisition assembly 2. A white light source 42 is provided at the center of the light source turntable 41. The white light source 42 uses a white light LED, and its irradiation direction is vertically upward and coaxial with the imaging acquisition assembly 2.

[0044] With comprehensive reference to Figure 1 , 5 , a plurality of pairs of monochromatic light sources 43 are provided on the light source turntable 41. Each pair of monochromatic light sources 43 is symmetric about the center of the light source turntable 41. In this embodiment, 6 pairs of monochromatic light sources 43 are provided. The two monochromatic light sources 43 in each pair are the same. The light-emitting end of the monochromatic light source 43 points to directly above the white light source 42, and the light paths cross and overlap on the surface of the sample to be inspected, which can improve the intensity of the excitation light. The central wavelength of the monochromatic light source 43 is one or more of 350nm ± 20nm, 494nm ± 20nm, 535nm ± 20nm, 585nm ± 20nm, 643nm ± 20nm, and 684nm ± 20nm.

[0045] As Figure 6 , 8 shown, a second filter 44 is provided at the light-emitting end of the monochromatic light source 43. The second filter 44 is a prior art and can be purchased commercially. A light homogenizing component 45 is provided between the monochromatic light source 43 and the second filter 44.

[0046] In practical applications, the white light source 42 and the monochromatic light source 43 are not turned on simultaneously. The white light source 42 is only turned on once before the start of a single experiment; the light paths emitted by a pair of two identical monochromatic light sources 43 overlap in the target area of the sample to be inspected; each emission filter (i.e., the first filter 3) corresponds to each monochromatic light source 43 one by one. When the monochromatic light source 43 is turned on, the corresponding emission filter will be switched to.

[0047] Specifically, as Figure 1 shown, the imaging acquisition assembly 2 includes a camera 21 and a telecentric lens 22 arranged in sequence from top to bottom. The camera 21 is used for imaging the sample to be inspected and collecting fluorescence signals. The telecentric lens 22 is used to adjust the imaging target area. The camera 21 is mounted on the bracket 1 through an L-shaped mounting seat. The telecentric lens 22 is coaxial with the camera 21 and vertically downward.

[0048] As Figure 3As shown in the figure, the fluorescence detection system for a biochip further includes a filter wheel 31 rotatably mounted on a bracket 1. A filter wheel drive mechanism 32 capable of driving the filter wheel 31 to rotate is provided on the bracket 1. A plurality of first filters 3, six in this embodiment, are respectively provided corresponding to 6 pairs of monochromatic light sources 43 one by one. All the first filters 3 are distributed on the filter wheel 31 in a circular array. The central wavelength and bandwidth of the first filter 3 are one or more of 440nm ± 30nm, 518nm ± 30nm, 556nm ± 30nm, 605nm ± 30nm, 667nm ± 30nm, and 710nm ± 30nm.

[0049] The filter wheel drive mechanism 32 can drive the filter wheel 31 to drive the first filter 3 to rotate below the imaging acquisition assembly 2. The first filter 3 rotated below the imaging acquisition assembly 2 is coaxial with the imaging acquisition assembly 2. Different first filters 3 and different pairs of monochromatic light sources 43 can form different detection channels, and can be switched according to the selection of the detection channel, so that the emission filter (i.e., the first filter 3) matching the detection channel is located at the ends of the camera 21 and the telecentric lens 22.

[0050] The specific installation method of the first filter 3 on the filter wheel 31 is as follows: Six through holes are opened on the filter wheel 31, and the first filter 3 is installed in the corresponding through holes. The first filter 3 is a prior art and can be purchased commercially.

[0051] As Figure 4 shown in the figure, the filter wheel drive mechanism 32 uses a motor, the motor shaft is vertically downward, and the filter wheel 31 is installed on the motor shaft. The motor is installed on the bracket 1 through an L-shaped mounting seat.

[0052] As Figure 3 shown in the figure, the light source assembly 4 further includes a light source turntable drive mechanism 46, and the light source turntable drive mechanism 46 can drive the light source turntable 41 to rotate; the light source turntable drive mechanism 46 uses a motor, the motor shaft is vertically upward and coaxial with the imaging acquisition assembly 2, and the light source turntable 41 is installed on the motor shaft.

[0053] As Figure 1As shown, a number of pairs of fixing seats 47 are provided on the light source turntable 41. Each pair of fixing seats 47 is centrosymmetric about the center of the light source turntable 41. In this embodiment, 12 fixing seats 47 are provided, and the fixing seats 47 and the monochromatic light sources 43 are in one-to-one correspondence. The specific number can be increased or decreased according to actual needs. The inside of the fixing seat 47 is axially penetrated and has a cylindrical structure. The fixing seat 47 is inclined, and the inclination angle of the fixing seat 47 satisfies that the included angle between the axis of the fixing seat 47 and the vertical axes of the camera 21 and the telecentric lens 22 is 30° - 60°, preferably 47.5° ± 1.5°. It can be set according to actual needs during actual application.

[0054] As Figure 8 shown, the monochromatic light source 43 is arranged at the lower end of the fixing seat 47, the second filter 44 is arranged at the upper end of the fixing seat 47, and the light homogenizing component 45 is arranged inside the fixing seat 47.

[0055] Specifically, as Figure 8 shown, the light homogenizing component 45 includes an aspherical lens 451, a fly-eye lens 452, and a focusing lens 453 arranged in sequence along the light-emitting direction of the monochromatic light source 43. The aspherical lens 451, the fly-eye lens 452, and the focusing lens 453 are all prior arts and can be purchased commercially.

[0056] As Figure 6-8 shown, a lower fixing seat 48 is arranged at the lower end of the fixing seat 47. The upper part of the lower fixing seat 48 has a cylindrical structure, and the lower part is a square seat. The cylindrical structure at the upper part of the lower fixing seat 48 is provided with an external thread, and the inside of the lower end of the fixing seat 47 is provided with an internal thread. The lower fixing seat 48 is installed at the lower end of the fixing seat 47 through the thread.

[0057] As Figure 8 shown, the monochromatic light source 43 and the aspherical lens 451 are arranged on the lower fixing seat 48. Specifically, a light-transmitting hole 481 coaxial with the fixing seat 47 is arranged in the lower fixing seat 48, and the light-transmitting hole 481 is located between the monochromatic light source 43 and the aspherical lens 451. Specifically, the upper part of the light-transmitting hole 481 is a conical slope with a large upper part and a small lower part. A lower pressing ring 482 is arranged inside the upper end of the lower fixing seat 48. The lower pressing ring 482 is installed in the lower fixing seat 48 through the thread, and the aspherical lens 451 is pressed in the lower fixing seat 48 by the lower pressing ring 482.

[0058] As Figure 6 、 8 shown, a heat dissipation component 49 is arranged at the bottom of the lower fixing seat 48; as Figure 9 、 10As shown, the heat dissipation component 49 includes a plurality of heat dissipation fins arranged in parallel. The upper part of the heat dissipation component 49 is a square seat, and the heat dissipation fins are evenly distributed below the square seat. The cross-sectional sizes of the square seats of the heat dissipation component 49 and the lower fixed seat 48 are the same, and the two square seats are connected by screws. A groove is formed below the light transmission hole 481 for accommodating the monochromatic light source 43. The monochromatic light source 43 is fixedly installed in the groove or fixedly connected to the square seat of the heat dissipation component 49.

[0059] As Figure 8 shown, an installation seat 40 with a cylindrical structure is provided at the upper end of the fixed seat 47. The compound eye lens 452 is pressed by the installation seat 40 inside the fixed seat 47. An upper pressing ring 401 is provided inside the upper end of the installation seat 40, and the upper pressing ring 401 is threadedly connected to the inside of the upper end of the installation seat 40. The focusing lens 453 and the second filter 44 are pressed by the upper pressing ring 401 inside the installation seat 40. Specifically, parts of the edge of the second filter 44 bulge upward and downward respectively. After installation, a certain gap is provided between the second filter 44 and the top of the focusing lens 453.

[0060] In this embodiment, the monochromatic light source 43 uses a monochromatic LED. The light emission direction of the monochromatic light source 43 is coaxial with the fixed seat 47. The light transmission hole 481, the aspherical lens 451, the compound eye lens 452, the focusing lens 453, the second filter 44, and the fixed seat 47 are all coaxial.

[0061] Working principle:

[0062] When the fluorescence detection system for a biochip in the present invention is actually applied, the imaging acquisition component 2 is used for imaging and fluorescence signal acquisition of the sample to be detected. The first filter 3 can filter the emitted fluorescence in non-characteristic bands and separate the emitted fluorescence in characteristic bands. The white light source 42 can provide a bright field environment for focusing and imaging the sample to be detected. The monochromatic light source 43 can excite the fluorescent substances in the sample to be detected to generate fluorescent signals that can be detected by optical devices. The light homogenizing component 45 can enhance the uniformity of the light signal and improve the light energy utilization rate. The fluorescence detection system for a biochip provided by the present invention has a compact structure, greatly reducing the space occupation of the hardware, providing favorable conditions for the design and development of instrument portability and miniaturization. The coaxial cross-oblique shooting scheme of the imaging acquisition component 2, the white light source 42, and the monochromatic light source 43 proposed by the present invention can effectively reduce the energy loss during the transmission of the light signal, which is beneficial to the detection of weak fluorescent signals in the sample to be detected. The use of the white light source 42 for illumination to assist the imaging acquisition component 2 for focusing and imaging can provide favorable conditions for the high-precision capture of fluorescent signals in the sample to be detected. This system can simultaneously meet detection indexes such as high optical path uniformity, large detection field of view, and high resolution. Based on this system, the effective detection of fluorescent signals in nucleic acid or protein amplification biochips can be realized.

[0063] In actual application, the motor drives the filter turntable 31 to rotate, thereby driving different first filters 3 to rotate below the imaging acquisition assembly 2, which can meet the multi-channel detection requirements. The light homogenizing assembly 45 formed by the aspherical lens 451, the compound eye lens 452, and the focusing lens 453 can effectively enhance the uniformity of the optical signal and improve the utilization rate of light energy.

[0064] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A fluorescence detection system for a biochip, characterized in that: It includes a bracket (1) and an imaging acquisition component (2) vertically arranged on the bracket (1). A first filter (3) is arranged below the imaging acquisition component (2), and several first filters (3) are provided; A light source component (4) is arranged below the first filter (3). The light source component (4) includes a light source turntable (41). The light source turntable (41) is coaxial with the imaging acquisition component (2), and a white light source (42) is arranged at the center of the light source turntable (41); Several pairs of monochromatic light sources (43) are arranged on the light source turntable (41). Each pair of monochromatic light sources (43) is symmetric about the center of the light source turntable (41). The light-emitting end of the monochromatic light source (43) points to the directly above of the white light source (42). A second filter (44) is arranged at the light-emitting end of the monochromatic light source (43), and a light homogenizing component (45) is arranged between the monochromatic light source (43) and the second filter (44); The optical paths emitted by a pair of two same monochromatic light sources (43) overlap in the target area of the sample to be detected, and several first filters (3) correspond to several pairs of monochromatic light sources (43) one by one.

2. The fluorescence detection system for a biochip according to claim 1, characterized in that: It further includes a filter turntable (31) rotatably installed on the bracket (1), and a filter turntable driving mechanism (32) capable of driving the filter turntable (31) to rotate is arranged on the bracket (1). All the first filters (3) are distributed in a circular array on the filter turntable (31), and the filter turntable driving mechanism (32) can drive the filter turntable (31) to drive the first filter (3) to rotate below the imaging acquisition component (2); The filter turntable driving mechanism (32) uses a motor, the motor shaft is vertically downward, and the filter turntable (31) is installed on the motor shaft.

3. A fluorescence detection system for a biochip according to claim 1, wherein: The light source component (4) further includes a light source turntable driving mechanism (46), and the light source turntable driving mechanism (46) can drive the light source turntable (41) to rotate; The light source turntable driving mechanism (46) uses a motor, the motor shaft is vertically upward and coaxial with the imaging acquisition component (2), and the light source turntable (41) is installed on the motor shaft.

4. A fluorescence detection system for a biochip according to claim 1, wherein: Several pairs of fixing seats (47) are arranged on the light source turntable (41). Each pair of fixing seats (47) is symmetric about the center of the light source turntable (41). The inside of the fixing seat (47) is axially penetrated. The fixing seat (47) is inclined. The monochromatic light source (43) is arranged at the lower end of the fixing seat (47), the second filter (44) is arranged at the upper end of the fixing seat (47), and the light homogenizing component (45) is arranged inside the fixing seat (47).

5. The fluorescence detection system for a biochip according to claim 4, characterized in that: The light homogenizing component (45) includes an aspherical lens (451), a fly-eye lens (452), and a focusing lens (453) arranged in sequence along the light-emitting direction of the monochromatic light source (43).

6. The fluorescence detection system for a biochip according to claim 5, wherein: A lower fixing seat (48) is arranged at the lower end of the fixing seat (47), and the monochromatic light source (43) and the aspherical lens (451) are arranged on the lower fixing seat (48).

7. The fluorescence detection system for a biochip according to claim 6, characterized in that: A light-transmitting hole (481) coaxial with the fixed seat (47) is provided in the lower fixed seat (48), and the light-transmitting hole (481) is located between the monochromatic light source (43) and the aspherical lens (451).

8. A fluorescence detection system for a biochip according to claim 6, characterized in that: A lower pressing ring (482) is provided inside the upper end of the lower fixed seat (48), and the aspherical lens (451) is pressed by the lower pressing ring (482) in the lower fixed seat (48).

9. A fluorescence detection system for a biochip according to claim 6, characterized in that: A heat dissipation component (49) is provided at the bottom of the lower fixed seat (48); The heat dissipation component (49) includes a plurality of heat dissipation fins arranged in parallel.

10. A fluorescence detection system for a biochip according to claim 5, characterized in that: An installation seat (40) is provided at the upper end of the fixed seat (47), the fly-eye lens (452) is pressed by the installation seat (40) inside the fixed seat (47), an upper pressing ring (401) is provided inside the upper end of the installation seat (40), and the focusing lens (453) and the second filter (44) are pressed by the upper pressing ring (401) inside the installation seat (40).

Images