A rice male fertility control gene STS1, its encoding protein and application

Abstract

The present invention provides a rice male fertility control gene STS1, a protein encoded by the gene and an application thereof, and belongs to the field of plant biotechnology. The nucleotide sequence of the rice male fertility control gene STS1 has one of the following groups: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a DNA sequence having a similarity of not less than 90% with the nucleotide sequence as shown in SEQ ID NO.1 and having the same function; 3) a DNA sequence capable of hybridizing with the DNA of the sequence in 1) under certain conditions; 4) a DNA sequence complementary to any of the sequences in 1) to 3); the present invention also protects a method for affecting plant fertility by affecting the nucleotide sequence of STS1 or by regulating the transcriptional expression of the STS1 gene, and using conventional genetic transformation means to transfer the STS1 gene into the rice male sterile strain obtained by the aforementioned application, thereby restoring the mutant to the wild-type phenotype.

Description

Technical Field

[0001] The present invention relates to the field of plant gene technology, and in particular to a rice male fertility control gene STS1, a protein encoded by the gene and an application thereof. Background Art

[0002] Rice is one of the most important crops for human nutrition and calorie intake, providing more than one-fifth of the calories consumed by the entire human race. Utilizing hybrid vigor is the main means of breeding new rice varieties and an important way to increase rice yields. Rice is a self-pollinating plant, with pistils and stamens growing in the same small spikelet. Artificial emasculation is difficult to produce a large number of hybrid seeds required for production, so hybrid rice breeding relies on the exploration and utilization of male sterile germplasm resources. In other words, using this natural male sterile rice as the female parent and artificial assisted pollination, hybrid seeds can be produced in large quantities. However, for a long time, due to the overall limited exploration and utilization of male sterile germplasm resources, the currently widely used ones are limited to nuclear-cytoplasmic interaction male sterility (three-line) and temperature-sensitive nuclear sterility (two-line). The former is restricted by the restoration relationship, and the latter is easily affected by the environment. More importantly, the actual sterility gene resources in these two types are single, which limits the further improvement of the advantages of hybrid rice and also poses great risks. With the development of current biotechnology, recent studies have shown that by making full use of a technical means called "Seed Production Technology (SPT)", rice nuclear male sterile mutants can be directly applied in hybrid rice. Therefore, by isolating and cloning new types of nuclear male fertility control genes and creating new sterile materials, it is of great significance to expand the range of sterile line germplasm urgently needed in hybrid rice. Summary of the invention

[0003] In view of the problems and defects of the prior art, the present invention provides a rice male fertility control gene STS1, the protein encoded by it and its application. The gene and the protein encoded by it are used to regulate rice male fertility, and a new rice sterile line is obtained by mutating the encoding gene through gene editing, and the mutant fertility is restored through transgenic technology, which has important application value in agricultural production. The technical solution of the present invention is:

[0004] In the first aspect, the present invention provides a rice male fertility control gene STS1, which is derived from rice and has a nucleotide sequence having one of the following groups: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a DNA sequence having a similarity of not less than 90% with the nucleotide sequence as shown in SEQ ID NO.1 and having the same function; 3) a DNA sequence that can hybridize with the DNA of the sequence in 1) under certain conditions; 4) a DNA sequence that is complementary to any of the sequences in 1) to 3).

[0005] It is well known in the art that the rice male fertility control gene described in the present invention includes a highly homologous functional equivalent sequence that is highly homologous to the STS1 gene and has the same fertility control function. The highly homologous functional equivalent sequence includes a DNA sequence that can hybridize with the nucleotide sequence of the STS1 gene disclosed in the present invention under certain conditions. The "certain conditions" used in the present invention are common knowledge and include nucleic acid hybridization by means of denaturation (such as 90-96°C, a certain pH, and the presence of an organic solvent), annealing (under 40-65°C renaturation conditions), and extension (under the action of a polymerase at 68-75°C).

[0006] Functionally equivalent sequences also include DNA sequences that have at least 90%, 95%, 96%, 97%, 98%, or 99% sequence similarity to the sequence shown in the STS1 gene disclosed in the present invention and have fertility regulatory functions and can be isolated from any plant. Among them, the percentage of sequence similarity can be obtained by well-known bioinformatics algorithms, including Myers and Miller algorithm (Bioinformatics, 4 (1): 11-17, 1988), Needleman-Wunsch global alignment method (J. Mol. Biol., 48 (3): 443-53, 1970), Smith-Waterman local alignment method (J. Mol. Biol., 147: 195-197, 1981), Pearson and Lipman similarity search method (PNAS, 85 (8): 2444-2448, 1988), Karlin and Altschul algorithm (Altschul et al., J. Mol. Biol., 215 (3): 403-410, 1990; PNAS, 90: 5873-5877, 1993). This is familiar to those skilled in the art.

[0007] The above-mentioned "can be isolated and obtained from any plant" includes, but is not limited to, Brassica, corn, wheat, sorghum, two-joint genus, mustard, castor bean, sesame, cotton seed, linseed, soybean, Arabidopsis, bean, peanut, alfalfa, oat, rapeseed, barley, oat, rye, millet, milo, triticale, einkorn wheat, spelt, emmer wheat, flax, gramma grass, friction grass, teosinte, fescue, perennial wheat grass, sugarcane, cranberry moss, papaya, banana, safflower, oil palm, melon, apple, cucumber, dendrobium, gladiolus, chrysanthemum, lily family, cotton, eucalyptus, sunflower, Brassica, sugar beet, coffee, ornamental plants and pine, etc. Preferably, the plants include corn, soybean, safflower, mustard, wheat, barley, rye, rice, cotton and sorghum.

[0008] In a second aspect, the present invention also provides a biological material related to the rice male fertility control gene STS1, which is any one of the following 1) to 5):

[0009] 1) Encoding protein of gene STS1;

[0010] 2) an expression cassette containing the gene STS1;

[0011] 3) a recombinant vector containing the gene STS1;

[0012] 4) A recombinant microorganism containing the STS1 gene;

[0013] 5) A recombinant microorganism containing the recombinant vector described in 3).

[0014] Furthermore, the amino acid sequence of the protein encoding gene STS1 has a sequence as shown in SEQ ID NO.2, or an amino acid sequence that is similar to the amino acid sequence shown in SEQ ID NO.2 and has the same function.

[0015] Further, during the preparation of the expression cassette containing the gene STS1, various DNA fragments can be manipulated to provide a DNA sequence in a suitable direction or in a correct reading frame. To achieve this purpose, adaptors or joints can be used to connect the DNA fragments, or other operations can be further included to provide convenient restriction enzyme sites, etc. In addition, the expression cassette or recombinant vector provided by the present invention can be inserted into any vector in a plasmid, a cosmid, a yeast artificial chromosome, a bacterial artificial chromosome, or other suitable for transformation into a host cell. Preferred host cells are bacterial cells, especially bacterial cells used for cloning or storing polynucleotides, or for transforming plant cells, such as Escherichia coli, Agrobacterium tumefaciens and Agrobacterium rhizogenes. When the host cell is a plant cell, the expression cassette or recombinant vector can be inserted into the genome of the transformed plant cell. The insertion can be a localized or random insertion. Preferably, the insertion is achieved by, for example, homologous recombination. In addition, the expression cassette or recombinant vector can be maintained outside the chromosome. The expression cassette or recombinant vector of the present invention can be present in the nucleus, chloroplast, mitochondria and / or plastid of the plant cell. Preferably, the expression cassette or recombinant vector of the present invention is inserted into the chromosomal DNA of the plant cell nucleus.

[0016] Further, the recombinant microorganism can be obtained by conventional methods or gene editing means, such as operably connecting the promoter sequence to the reporter gene to form a transformable construct, and then transferring the construct into the plant; or the above-mentioned construct is subcloned into an expression vector for transient expression experiments, and the function of the promoter or its regulatory region is detected by transient expression experiments. The selection of an appropriate expression vector for testing the function of the promoter or regulatory region will depend on the host and the method for introducing the expression vector into the host, and such methods are well known to those of ordinary skill in the art. For eukaryotes, the region in the vector includes regions that control transcription initiation and control processing. These regions are operably connected to a reporter gene, and the reporter gene includes YFP, UidA, GUS gene or luciferase. The expression vector containing the putative regulatory region located in the genomic fragment can be introduced into intact tissues, such as stage pollen, or introduced into callus tissue.

[0017] In a third aspect, the application described in the present invention is: the present invention provides a method for affecting plant fertility by affecting the nucleotide sequence of STS1 or by regulating the transcriptional expression of the STS1 gene, thereby obtaining a new rice male sterile line, and the rice male sterile line can be used to produce hybrid seeds.

[0018] The method of influencing plant fertility refers to regulating the expression of the STS1 gene, thereby changing the fertility of the plant, such as causing male sterility of the plant. Specifically, depending on the specific application requirements, a variety of methods can be used to influence the expression of the STS1 gene in the plant, thereby achieving the effect of regulating the male fertility of the plant. More specifically, the expression of the STS1 gene can be regulated using many tools available to those of ordinary skill in the art, for example, mutation, mutagenesis, transfer of antisense genes, co-suppression or introduction of hairpin structures, and gene editing, etc., can all be used to destroy the normal expression of the STS1 gene, thereby obtaining male sterile plants.

[0019] In addition, the present invention also provides a sterile mutant sequence of the STS1 gene and a male sterile mutant material thereof. More specifically, the male sterile mutant material is a process in which the plant loses male fertility by mutating the endogenous STS1 gene of rice, or mutating the nucleotide sequence of a gene highly homologous thereto. The "mutation" includes but is not limited to the following methods, such as gene mutation caused by physical or chemical methods, chemical methods including mutagenesis caused by treatment with mutagens such as EMS, the mutation can also be a point mutation, a DNA deletion or insertion mutation, or can be produced by gene silencing and gene editing such as RNAi and site-directed mutagenesis.

[0020] Those skilled in the art should be aware that the male sterile material receptors created may include, but are not limited to, all conventional indica and japonica rice varieties currently known.

[0021] Specifically, the present invention uses the above method to construct four rice male sterile mutants, and the four rice male sterile mutants all contain mutated male sterile genes, and the nucleotide sequences of the mutated male sterile genes are shown in SEQ ID NO.6 (sts1-1), SEQ ID NO.7 (sts1-2), SEQ ID NO.8 (sts1-3), and SEQ ID NO.9 (sts1-4). Compared with the wild type, in the four male sterile mutants, the nucleotide of a splicing recognition site in the intron of the coding region of the gene is replaced in the sts1-1 mutant, resulting in three erroneous transcripts; a nucleotide in the 7th exon of the gene is replaced in the sts1-2 mutant, thereby causing the terminator to be formed prematurely; a base is inserted in the 2nd exon of the gene in the sts1-3 mutant, thereby causing the terminator to be formed prematurely; and two bases are deleted in the 9th exon of the gene in the sts1-4 mutant, thereby causing the terminator to be formed prematurely. Mutations in this gene in the four mutants ultimately led to premature termination of protein translation.

[0022] In a fourth aspect, the present invention also relates to a method for restoring the male sterility trait of a rice male sterile line, comprising the following steps: using conventional genetic transformation means to transfer the STS1 gene into the rice male sterile line obtained by the application as described above, thereby restoring the mutant to a wild-type phenotype.

[0023] In a fifth aspect, the present invention also relates to a use of a rice sterile line in rice seed production, using the rice male sterile mutant line constructed as described above as a female parent for hybrid breeding. Specifically, in certain application embodiments, the STS1 gene provided by the present invention can be used to achieve the reproduction and maintenance of male sterile lines obtained by mutations in STS1 or other similar fertility-related genes.

[0024] More specifically, the reproduction and maintenance of the male sterile line refers to using a homozygous recessive nuclear male sterile mutant as a transformation recipient material, and transforming three tightly linked target genes into the sterile mutant recipient plant. The three target genes are fertility restoration gene, pollen inactivation gene and screening gene. Among them, the fertility restoration gene can restore the fertility of the sterile transformation recipient, the pollen inactivation gene can inactivate the pollen containing the transformed exogenous gene, that is, lose the fertilization ability, the screening gene can be used for sorting transgenic seeds and non-transgenic seeds, the sorted non-transgenic seeds are used as sterile lines to produce hybrids, and the transgenic seeds are used as maintenance lines to continuously and stably produce sterile lines. The pollen-specific expression promoter provided by the present invention can be used for the specific expression of exogenous genes in pollen, thereby avoiding the adverse effects caused by the continuous expression of the exogenous genes in other tissues of the plant, and can also be used for the functional analysis and identification of genes related to plant pollen growth and development; it can be used for the creation of male sterile lines and restorer lines; and it can be applied to pollen abortion experiments, thereby avoiding the biosafety problems caused by plant transgenic drift or pollen escape, which is of great significance to the creation of plant male sterile lines and restorer lines.

[0025] Those skilled in the art should know that suitable screening genes include, but are not limited to, chloramphenicol resistance gene, hygromycin resistance gene, streptomycin resistance gene, spectinomycin resistance gene, sulfonamide resistance gene, glyphosate resistance gene, and oxaliplatin resistance gene. The selection marker gene can also be a red fluorescent gene, a cyan fluorescent protein gene, a yellow fluorescent protein gene, a luciferase gene, a green fluorescent protein gene, anthocyanin p1 and other genes.

[0026] In addition, the transgenic plants of the present invention are prepared using transformation methods known to those skilled in the art in the field of plant biotechnology. Any method can be used to transform the recombinant expression vector into a plant cell to produce the transgenic plants of the present invention. The transformation method can include direct and indirect transformation methods. Suitable direct methods include polyethylene glycol-induced DNA uptake, liposome-mediated transformation, introduction using a gene gun, electroporation, and microinjection, etc. In a specific embodiment of the present invention, the present invention uses a transformation technique based on soil agrobacterium.

[0027] Compared with the prior art, the present invention can achieve the following technical effects:

[0028] 1) The present invention obtains a rice male reproductive development mutant by controlling the rice male fertility STS1 gene and its encoded protein, thereby achieving artificial control of the rice reproductive process.

[0029] 2) The rice mutant obtained by the present invention has no obvious difference from the source parent during the vegetative stage. After entering the reproductive growth stage, the male reproductive development is abnormal and the pollen is aborted, thereby obtaining a sterile line with complete male sterility. The fertility of the sterile line is stable, not affected by environmental conditions, and can be restored by wild-type transgenics.

[0030] 3) This gene and the sterile line produced by this gene mutation provide the necessary elements for constructing the third-generation hybrid breeding system. The male sterile line produced by this gene mutation is used to produce hybrid seeds, which is of great significance for breaking through and improving the existing "three-line" and "two-line" hybridization technologies, and has very important application value in hybrid rice construction and agricultural production.

[0031] Of course, any product implementing the present invention does not necessarily need to achieve all of the above-mentioned technical effects at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The phenotype diagram of the STS1 gene after mutation in Example 1 of the present invention; wherein A is the wild type 9311 and mutant plant type; B is the wild type and mutant panicle; C is the wild type and mutant floret; D is the wild type and mutant anther morphology; E is the mutant and wild type pollen iodine staining.

[0033] Figure 2 It is the clone of the STS1 gene in Example 2 of the present invention; wherein A and B are the SNP index maps of mutants sts1-1 and sts1-2, respectively; C is the gene structure and mutation sites and modes of the STS1 gene; D is the three erroneous transcripts produced by the mutation in sts1-1; E is the co-segregation verification of the mutation sites and phenotypes of mutants sts1-1 and sts1-2.

[0034] Figure 3 The figure is a mutant phenotype diagram obtained by gene editing of the STS1 gene in Example 3 of the present invention; wherein A is the gene structure of the STS1 gene and the gene editing sites of the mutants sts1-3 and sts1-4 and the base changes after successful editing; B is the plant type of the wild type Nipponbare and the mutants sts1-3 and sts1-4; C is the anther morphology of the wild type Nipponbare and the mutants sts1-3 and sts1-4; D is the pollen iodine staining of the wild type Nipponbare and the mutants sts1-3 and sts1-4.

[0035] Figure 4It is a phenotypic diagram of the sts1-1 mutant fertility restoration strain in Example 4 of the present invention; wherein A is the STS1 gene structure for restoring fertility; B is the plant type of the wild type 9311, mutant and complementation strain; C is the floret of the wild type 9311, mutant and complementation strain; D is the anther morphology of the wild type 9311, mutant and complementation strain; E is the pollen iodine staining of the wild type 9311, mutant and complementation strain. DETAILED DESCRIPTION

[0036] In the description of the present invention, it should be noted that, if the specific conditions are not specified in the examples, the experiments were carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, if the manufacturer is not specified, are all conventional products that can be purchased commercially.

[0037] The inventors used bioinformatics techniques well known to those skilled in the art to identify a complete rice male fertility control gene STS1 from the rice genome, the nucleotide sequence of which is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO.2; the full-length ORF of the STS1 gene is 1986bp in length and encodes 661 amino acids.

[0038] The present invention is further described in detail below in conjunction with the accompanying drawings and specific embodiments, which are intended to explain the present invention rather than to limit it.

[0039] Example 1

[0040] Phenotypes of STS1 mutations

[0041] The conventional gene mutation technology in the art was used to induce mutation in the conventional rice variety 9311. Two male sterile mutants sts1-1 and sts1-2 were found in the mutant library. The nucleotide sequences were shown in SEQ ID NO.6 and SEQ ID NO.7, respectively. The two mutants were stably inherited after multiple generations of hybridization with the wild type (WT). Phenotypic analysis showed that the mutants showed similar vegetative growth to the wild type, but could not produce mature pollen grains, showing pollenless male sterility ( Figure 1 ). The same gene STS1 was further cloned from the two mutants by genome resequencing technology. The specific steps are shown in Example 2.

[0042] Example 2

[0043] Cloning of STS1 gene

[0044] 1. Resequencing cloning of STS1 gene mutations

[0045] Two mutants sts1-1 and sts1-2 were used as female parents to cross with the wild type, and segregation populations were constructed. Male sterile plants and normal fertile plants in the segregation populations were mixed and sequenced separately. The sequencing data were compared with the wild type genome to obtain a map with a SNP index of 1 (such as Figure 2 As shown in Figures A and B in the figure). The variant sites in the above map were further analyzed, and the single strains and variant sites isolated in the population were co-segregated and verified by PCR sequencing, and the only SNP variant sites that can be co-segregated with the phenotype were obtained. The primers for verifying the site were sts1-1F (SEQ ID NO.10), sts1-1R (SEQ ID NO.11) and sts1-2F (SEQ ID NO.12), sts1-2R (SEQ ID NO.13). The gene where the site is located is STS1. Figure 2 As shown in Figures C, D, and E, the variation patterns of sts1-1 and sts1-2, the three erroneous transcripts produced by the mutation in sts1-1, and the co-segregation analysis of sts1-1 and sts1-2. It is thus confirmed that sts1-1 and sts1-2 are different variations occurring on the same gene STS1. Among them, a nucleotide substitution occurred at a splicing recognition site in the intron of the coding region of the STS1 gene, resulting in a DNA sequence as shown in SEQ ID NO.6, leading to the production of three misplaced transcripts, thereby obtaining the sts1-1 mutant; a nucleotide substitution occurred in the 7th exon of the coding region of the STS1 gene, resulting in a DNA sequence as shown in SEQ ID NO.7, leading to the premature formation of the terminator and the premature termination of protein translation, thereby obtaining a male sterile strain, namely the sts1-2 mutant.

[0046] The sequence of the wild-type STS1 gene is shown in SEQ ID NO. 1, and the encoded protein sequence is shown in SEQ ID NO. 2. The full-length ORF of the STS1 gene is 1986 bp in length, encoding 662 amino acids.

[0047] 2. Full-length cDNA cloning of STS1 gene

[0048] The RNA of the normal wild-type rice variety Nipponbare was used as a template to synthesize the first-strand cDNA, and the oligonucleotides at the 5' and 3' ends of the ORF sequence of the STS1 gene were used as PCR primers (SEQ ID NO.14 and SEQ ID NO.15), and the high-fidelity Taq enzyme Primerstar was used for amplification to obtain a full-length cDNA amplification product of the MFS1 gene of about 1.9K. The amplification product was recombined into the corresponding restriction endonuclease multiple cloning site of the vector pEntry through EcoRI restriction enzyme digestion, and the recombinant was sequenced and verified. The recombinant transitional plasmid vector is called pEntry-STS1.

[0049] The oligonucleotide sequence at the 5' end is (SEQ ID NO.14):

[0050] 5'-aaaaaagcaggctccgaattcatggccacgacgctgacg-3'

[0051] The oligonucleotide sequence at the 3' end is (SEQ ID NO.15):

[0052] 5'-caagaaagctgggtcgaattcttactgtgcaactgttccatctgtt-3'

[0053] Example 3

[0054] Creation of new STS1 mutant strains using gene editing

[0055] The commercial CRSPR / Cas9 gene editing vector BGK030 (commercially available) was used as a rice transgenic vector. The vector encodes a bacterial replication origin (ori), a kanamycin resistance gene (Kanamycin r ), hygromycin resistance gene (Hyg r ), gene editing gene (Cas9), 35S promoter, NOS gene termination signal sequence and multiple cloning site (MCS) for connecting target fragments. Two guide sequences SG RNAs of STS1 gene were inserted into the restriction endonuclease sites, the sequences are shown in SEQ ID NO.16 and SEQ ID NO.17, and transgenic plasmids BGK030-STS1-3 and BGK030-STS1-4 were constructed.

[0056] 4. Experiment on STS1 gene transformation into rice

[0057] The above BGK030-STS1-3 and BGK030-STS1-4 were introduced into Agrobacterium EHA105 by freeze-thaw method, and infected the callus tissue of wild-type japonica rice variety Nipponbare, cultured, and differentiated to obtain transgenic plants. Hygromycin was used to screen the transformed plants; total leaf DNA was extracted from positive plants, and the transformed plants were further identified by PCR. The fertility phenotype was investigated using the transgenic T1 generation to verify the function of the STS1 gene. Figure 3As shown in the figure, the plants with one base inserted and two bases deleted in the STS1 target site showed male sterility, and the obtained mutants were sts1-3 and sts1-4, respectively, and the nucleotide sequences were shown in SEQ ID NO.8 and SEQ ID NO.9, respectively. That is, the sts1-3 mutant had one nucleotide inserted in the second exon of the coding region of the STS1 gene; the sts1-4 mutant had two nucleotides deleted in the ninth exon of the coding region of the STS1 gene.

[0058] Example 4

[0059] Fertility restoration of STS1 gene mutant

[0060] The STS1 gene complementary expression vector was constructed. In this example, pCMBIYA1300 was firstly restricted with PstⅠ and SacⅠ, and the wild-type STS1 complementary fragment containing the promoter, about 6.3K, amplified using SEQ ID NO.3 and SEQ ID NO.4, and the sequence is shown in SEQ ID NO.5, was connected to the PstⅠ and SacⅠ sites of pCMBIYA1300, and the recombinant was digested and sequenced for identification. Then, the transgenic method described in Example 3 was used to obtain the rice STS1 complementary expression mutant transgenic plants, and the transgenic T1 generation plants were observed, as shown in FIG. Figure 4 As shown, in the mutant sts1-1, the transgenic positive plants obtained after successful transfer of the complementation expression vector restored fertility.

[0061] In summary, the present invention uses cloning technology and gene editing methods to change, increase or delete individual nucleotide sequences of STS1, resulting in loss of the encoded protein function, thereby obtaining male sterile lines. The fertility of the male sterile lines produced is complete and not affected by environmental conditions, and the transfer of wild-type genes can restore fertility. The male fertility control gene provided by the present invention and the sterile lines produced based on the gene will provide the necessary sterile line germplasm resources for the new hybrid breeding system, which is of great significance to hybrid rice breeding.

[0062] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims. Sequence Listing <110> Sichuan Agricultural University <120> A rice male fertility control gene STS1, its encoding protein and application <160> 17 <170> SIPOSequenceListing 1.0 <210> 1 <211> 1986 <212> DNA <213> STS1 gene (Oryza sativa) <400> 1 atggccacga cgctgacgcc gacgcagcgc tacgcggcgg gggcgctgct ggcgctcgcg 60 ctccgccagg cccagatcca ccagtccgtc ctcctcggcg cccaccacca ccacgacgac 120 gacgacgagg aacaggggcg caccagcacc agcagcggcg gcggcggcgg cagctcctcc 180 tcctcctcca actccggcgc cggcgccgac gccgacctct ggacacacga ctcccacggc 240 ctcctccgcc ccgtcttcag gttcctggag atcgatccca aggcgtggtc ggggctggag 300 gagacggcgg cgtcgtccga ggccaagcat cacatcggcg cgtttctaag gataatattc 360 gaagaagatg gcgaaagctc ctcagacaga tccgttcagg agcttgcctt ggcaaaagga 420 gttgatgtga tggtaatgag tttgggcaat gacagtgaag tgggtaacac aattaaaggt 480 ggggatcaag atgctttgcc tagcagctct ggaacagata aatccccagg ggaaagctct 540 catgatgacc agttaggaat caataaattg accttagatg atattcctgc taacaatcac 600 cggaagatgg cattgctgtt tgctcttctc tcggcttgtg ttgctgataa acctgtatca 660 caagagaag agcagaaa atcaacccgc ttcagaaagg gttatgatgc tcggcatcgt 720 gttgctcttc ggctgctatc tacatggctt gatgtgaagt ggatcaaaat ggaagcaatt 780 gaggttatgg ttgcatgctc tgccatggca gcagcgaaag aacaagaaca atcacaagaa 840 agcgcatcac ccaaaagtaa atgggaaaaa tggaagcgtg gaggaataat tggtgcagct 900 gctttgactg gagagcact gttggctatt actgggggtt taggctctcc agcaattgct 960 gcagggtttg gtgctcttgc tccaaccctg ggcacacttg tccctgttat aggagctagc 1020 ggatttgctg ctatggctac cgctgcagga tctgttgctg gctctgtggc agttgctgca 1080 tcatttggag ctgctggagc tggcttgacg gggagcaaaa tggccagaag aattgggagc 1140 gtgaaagaat ttgagttcaa acctattggt gaaaaccata atcagggtcg gcttgcagtt 1200 ggcatcttaa tttccggatt tgcttttgat gaggatgact tttgtagacc ttgggaagga 1260 tggcaggata acctagagag gtacatcctt caatgggagt ctaagcatat aattgcagtg 1320 agtacagcaa tacaggattg gctgacatca agacttgcta tggaattgat gaagcaaggt 1380 gcaatgagaa ccgtattaag tggtcttctt gcagcatttg catggccagc tacactgctt 1440 gcggctacag actttattga tagtaaatgg tctgttgcta ttgacagatc agataaggca 1500 ggaaaaatgc ttgctgaagt gcttctcaag ggattgcaag gaaataggcc tgtgactctc 1560 attgggtttt cactaggtgc acgtgttata tttaagtgtt tacaggaact tgctttatcg 1620 agtgataatg agggacttgt tgagagggtg gttctttcttg gtgctccagt ttctgtgaaa 1680 ggcgagcggt gggaggctgc aaggaagatg gttgctggaa gatttgtgaa tgtgtactcc 1740 acagatgact ggattcttgg tgttactttt cgtgcgagtc tgctaaca agggttggct 1800 ggcatccagg ccattgatgt ccctggtgtt gaaaatgtcg atgttacgga gcttgtcgat 1860 ggccattcgt cttacctgtc ggcagcacaa cagatactgg agcatcttga actaaatacc 1920 tactatccgg ttttgttcc cctgagcgca gccaacgaag aaacagatgg aacagttgca 1980 written 1986 <210> 2 <211> 661 <212> PRT <213> Encoded protein of STS1 gene (Oryza sativa) <400> 2 Met Ala Thr Thr Leu Thr Pro Thr Gln Arg Tyr Ala Ala Gly Ala Leu 1 5 10 15 Leu Ala Leu Ala Leu Arg Gln Ala Gln Ile His Gln Ser Val Leu Leu 20 25 30 Gly Ala His His His His Asp Asp Asp Asp Glu Glu Gln Gly Arg Thr 35 40 45 Ser Thr Ser Ser Gly Gly Gly Gly Gly Ser Ser Ser Ser Ser Ser Asn 50 55 60 Ser Gly Ala Gly Ala Asp Ala Asp Leu Trp Thr His Asp Ser His Gly 65 70 75 80 Leu Leu Arg Pro Val Phe Arg Phe Leu Glu Ile Asp Pro Lys Ala Trp 85 90 95 Ser Gly Leu Glu Glu Thr Ala Ala Ser Ser Glu Ala Lys His His Ile 100 105 110 Gly Ala Phe Leu Arg Ile Ile Phe Glu Glu Asp Gly Glu Ser Ser Ser 115 120 125 Asp Arg Ser Val Gln Glu Leu Ala Leu Ala Lys Gly Val Asp Val Met 130 135 140 Val Met Ser Leu Gly Asn Asp Ser Glu Val Gly Asn Thr Ile Lys Gly 145 150 155 160 Gly Asp Gln Asp Ala Leu Pro Ser Ser Ser Gly Thr Asp Lys Ser Pro 165 170 175 Gly Glu Ser Ser His Asp Asp Gln Leu Gly Ile Asn Lys Leu Thr Leu 180 185 190 Asp Asp Ile Pro Ala Asn Asn His Arg Lys Met Ala Leu Leu Phe Ala 195 200 205 Leu Leu Ser Ala Cys Val Ala Asp Lys Pro Val Ser Gln Glu Glu Glu 210 215 220 Asp Arg Lys Ser Thr Arg Phe Arg Lys Gly Tyr Asp Ala Arg His Arg 225 230 235 240 Val Ala Leu Arg Leu Leu Ser Thr Trp Leu Asp Val Lys Trp Ile Lys 245 250 255 Met Glu Ala Ile Glu Val Met Val Ala Cys Ser Ala Met Ala Ala Ala 260 265 270 Lys Glu Gln Glu Gln Ser Gln Glu Ser Ala Ser Pro Lys Ser Lys Trp 275 280 285 Glu Lys Trp Lys Arg Gly Gly Ile Ile Gly Ala Ala Ala Leu Thr Gly 290 295 300 Gly Ala Leu Leu Ala Ile Thr Gly Gly Leu Ala Ala Pro Ala Ile Ala 305 310 315 320 Ala Gly Phe Gly Ala Leu Ala Pro Thr Leu Gly Thr Leu Val Pro Val 325 330 335 Ile Gly Ala Ser Gly Phe Ala Ala Met Ala Thr Ala Ala Gly Ser Val 340 345 350 Ala Gly Ser Val Ala Val Ala Ala Ser Phe Gly Ala Ala Gly Ala Gly 355 360 365 Leu Thr Gly Ser Lys Met Ala Arg Arg Ile Gly Ser Val Lys Glu Phe 370 375 380 Glu Phe Lys Pro Ile Gly Glu Asn His Asn Gln Gly Arg Leu Ala Val 385 390 395 400 Gly Ile Leu Ile Ser Gly Phe Ala Phe Asp Glu Asp Asp Phe Cys Arg 405 410 415 Pro Trp Glu Gly Trp Gln Asp Asn Leu Glu Arg Tyr Ile Leu Gln Trp 420 425 430 Glu Ser Lys His Ile Ile Ala Val Ser Thr Ala Ile Gln Asp Trp Leu 435 440 445 Thr Ser Arg Leu Ala Met Glu Leu Met Lys Gln Gly Ala Met Arg Thr 450 455 460 Val Leu Ser Gly Leu Leu Ala Ala Phe Ala Trp Pro Ala Thr Leu Leu 465 470 475 480 Ala Ala Thr Asp Phe Ile Asp Ser Lys Trp Ser Val Ala Ile Asp Arg 485 490 495 Ser Asp Lys Ala Gly Lys Met Leu Ala Glu Val Leu Leu Lys Gly Leu 500 505 510 Gln Gly Asn Arg Pro Val Thr Leu Ile Gly Phe Ser Leu Gly Ala Arg 515 520 525 Val Ile Phe Lys Cys Leu Gln Glu Leu Ala Leu Ser Ser Asp Asn Glu 530 535 540 Gly Leu Val Glu Arg Val Val Leu Leu Gly Ala Pro Val Ser Val Lys 545 550 555 560 Gly Glu Arg Trp Glu Ala Ala Arg Lys Met Val Ala Gly Arg Phe Val 565 570 575 Asn Val Tyr Ser Thr Asp Asp Trp Ile Leu Gly Val Thr Phe Arg Ala 580 585 590 Ser Leu Leu Thr Gln Gly Leu Ala Gly Ile Gln Ala Ile Asp Val Pro 595 600 605 Gly Val Glu Asn Val Asp Val Thr Glu Leu Val Asp Gly His Ser Ser 610 615 620 Tyr Leu Ser Ala Ala Gln Gln Ile Leu Glu His Leu Glu Leu Asn Thr 625 630 635 640 Tyr Tyr Pro Val Phe Val Pro Leu Ser Ala Ala Asn Glu Glu Thr Asp 645 650 655 Gly Thr Val Ala Gln 660 <210> 3 <211> 19 <212> DNA <213> Artificial Sequence <400> 3 gctcatcttc gggctcacc 19 <210> 4 <211> 20 <212> DNA <213> Artificial Sequence <400> 4 gacacggcca gaaacagcaa 20 <210> 5 <211> 6353 <212> DNA <213> STS1 gene (Oryza sativa) <400> 5 gctcatcttc gggctcacca aggacgtcat ctttcgcgcc gccttcggca cgcgcgacgg 60 cggcggccac ggcgagctgg aggttctcct gcaggagttc tccaagctgt tcggcgcgtt 120 caacgtcggg gacttcatcc cctggctcgc gtggctcgac ccgcacggca tcaaccgacg 180 gctccgcgcg gcgcgcgccg ccctcgacag cgtcatcgac aggatcatcg acgagcacgt 240 cagcaacccc gccggcgacg aggacgccga catggtggac gacatgctcg cgttcctcga 300 cgaggccgga cgagaccaaa ccggcggcgg cggcgagctg cagggcacgc tccggctgac 360 gcgcgacaac atcaaggcca tcataatggt acgtgccatt ctgacaccac tgccatggca 420 tgcacgtcgc gttgcttgat ggagttggcg acgacaagtg caggacttcg tcttcggcgg 480 gacggagacg gtggcgtcgg cgatcgagtg ggcgatggcg gagttgctgc acagccccgg 540 cgatctccgg cggctgcagg cggagctcgc cgacgtggtg gggctcgggc gcggcgtgga 600 ggagggcgac ctggagaagc tccccttcct caggtgcgtc gccatggaga cgctgcgtct 660 ccacccgccg atcccgctgc tcctccacga ggcggcggcg gactgcgtcg tcggcgggta 720 ctccgtgccg aggggcgccc gcgtggtggt caacgtgtgg agcgtcggcc gcgacgccgg 780 cgcgtggaag ggcgacgccg gcgcgttccg gccggcgagg ttcatggcgg gcggcgaggc 840 ggcggggatg gacctcaggg gcggatgctt cgagctcctg ccgttcgggt cggggcggag 900 ggcgtgcccc gccatcgtgc tcgggatgta cgagctggag ctcgtcgtcg cgcgcctcgt 960 ccacgcgttc gggtgggcgc cgccgggcgg cgtggcgccg gaggagctcg acatggcgga 1020 cggcttcggc ctcaccgcgc cgcgcgccgc gaggctccgc gccgtgccga cgccccggct 1080 cacctgcccg atgtgacgcg cgttctcgat ttggcgcgag agatgcgcaa cgcagctcga 1140 agtgtgactg tgacagtgtg agcgtgtaat ctggtgtgga catcgtatgc atcatgtgtg 1200 cttatggata attaaatata taaataatat ttggatgtgg atttcggctg aaattttctg 1260 tcatttcgaa aaaaattcgt cctagaattc aaatagaata ttttttttct cattattttt 1320 tatagttgca cattggctac attatctgta gaagcttctt gattcacgag atctagcggg 1380 cgtgacaata tggtcaaaga attctttacc ctagacatgg gcgtcgatat ggtcgtaaag 1440 ctgctcttca gcttagtagc cacatccata atttaatctt aggattattt ttacagttga 1500 ttccgatatt ttttcatcgt aaacatgggg ttttacatca ttaaaaaccc acatataaaa 1560 tcctttccct taaaagaatg atggtctttg aagcccttat ttcttttttt aatcttcgag 1620 tatgtatgaa tagttgatt acgtgcattt taattatgta gagatttgga atgctattcg 1680 acattatat tatcttaatg attacattag tgagatgctt tattat aaaatcgatt 1740 atattttaaa aaaatctg aagcactccg tggaccgtgg tacacgtgc caggtcgacg 1800 tgttctctg gagaaatct atgtctactt tgaatgga atatactacc tccgttaaaaa 1860 ataagtaca acgtaata ataagtaca acgtaaat ccgtatgac gttcgtctta 1920 ttccaaaaat ttccaaaaat aaattataaaaaacagtca catataaaat attatttatg 1980 ttttataatc tattagtac aaaaaaat tatcataa agataaga aaatttaat 2040 aagataacg ctgaataata atattatttt aaagctgt acttatttta agacggaag 2100 agtaaaaaat tttggaaaaa tttctagtgc agcatgctgc tcattggga gtgtgtgct 2160 gctgctctc agtctccccc aaccaaaatt tgaccgagtt tggcacgaaa gaaaccgcct 2220 cgacgccgtc caacggaagc cagccgaggc gaccaaccca agggtaggcc taggcgtaaa 2280 ccctgcacct gatcgatcg ccggcgatgg ccacgacgct gaccccgacg cagcgctacg 2340 cggcgggggc gctgctggcg ctcgcgctcc gccaggccca gatccaccag tccgtcctcc 2400 tcggcgccca ccaccaccac gacgacgacg acgaggaaca ggggcgcacc agcaccagca 2460 gcggcggcgg cggcggcagc tcctcctcct cctccaactc cggcgccggc gccgacgccg 2520 acctctggac acacgactcc cacggcctcc tccgccccgt cttcaggtgt tttctcgctc 2580 ctctccgctt gtaaatttgg ggggtttgag atgaatgctt ccgctttact tggcgatcgg 2640 ggggctctgc taggttcctg gagatcgatc ccaaggcgtg gtcggggctg gaggagacgg 2700 cggcgtcgtc cgaggccaag catcacatcg gcgcggtacg gcactcactg cattttctgc 2760 cctcgtattg taattggccg tgcgttttgg tcagtgttgc agtgatggtt tggggcttag 2820 gtgtttgatt atatgcctac aagactgttc aacttcaatt tccgcgtgtg cttgaccagt 2880 gaaatttgtg ttttcgtagc atctcatatc actctcattt tgtagtttct aaggataata 2940 ttcgaagaag atggcgaaag ctcctcagac agatccgttc aggagcttgc cttggcaaaa 3000 ggagttgatg tgatggtaat gagtttgggc aatgacagtg aagtgggtaa cacaattaaa 3060 ggtggggatc aagatgcttt gcctagcagc tctggaacag ataaatcccc aggggaaagc 3120 tctcatgatg accagttagg aatcaataaa ttgaccttag atgatattcc tgctaacaat 3180 3240 tcacaagagg aagaggacag aaaatcaacc cgcttcagaa agggtttga tgctcggcat 3300 cgtgttgctc ttcggctgct atctacatgg cttgatgtga agtggatcaa aatggtttgt 3360 agactaaa ctttttttgga ttcagcagag taatcactat gggtaccaaa aatatccta 3420 aggcaattaa agaaaaaaga acccccaatg tttttcatgt ttcaccaaat ttgtcaccaa 3480 ccatgctttt tcgctgcagg aagcaattga ggttatggtt gcatgctctg ccatggcagc 3540 agcgaaagaa caagaacaat cacaagaaag cgcatcaccc aaagtaaat gggaaaaatg 3600 gaagcgtgga ggaataattg gtgcagctgc tttgactgga ggagcactgt tggctattac 3660 tggggtatg aaaaaacttc agaaattctg ctggacgctg aacatctgaa ctagtttctg 3720 tcgctcatat tactctaaac ggttgatatt ctcttccaac ttgacaggtt taggctctcc 3780 agcaattgct gcagggtttg gtgctcttgc tccaaccctg ggcacacttg tccctgttat 3840 aggagctagc ggatttgctg ctatggctac cgctgcagga tctgttgctg gctctgtggc 3900 agttgctgca tcatttggag gtgtgtctac atgttcttca aaacccttct ggtgatgttg 3960 ttttgagctt tcgtttcgtc atgctacatt actatcagct gcagtctccc tttccttttg 4020 tcatgagggt tgcaatattg tttagtgaac ttacccaaga atatgcacac aacactttta 4080 ggggtgcatt ttacaattag ataatagttc gatggattac cagaagaagg ataagactta 4140 tacaatgtat gacccaaaat tgtgagaaac atatggatga aagcgtaatg agaatagttg 4200 tggtgtggaa attttctatt tattcatgct tatcctttga aatgatattt ttcaattagc 4260 tgctggagct ggcttgacgg ggagcaaaat ggccagaaga attgggagcg tgaaagaatt 4320 tgagttcaaa cctattggtg aaaaccataa tcagggtgtg agttcctttt ctctttctga 4380 tgtcacaaca aatttcattc tactagattt ttgttcaccc ttttagtagt caaactgatc 4440 tggtctggtt aaatgttctc aacactttca gcggcttgca gttggcatct taatttccgg 4500 atttgcttt gatgaggatg acttttgtag accttgggaa ggatggcagg ataacctaga 4560 gaggtataga gtggttttt tgacatgttc ttttgctga tcactattct agtactttgt 4620 catgtgttac cagctaagag catccttaaa aatataattt caagagttga ataatgtctg 4680 gcaggtacat ccttcaatgg gagtctaagc atataattgc agtgagtaca gcaatacagg 4740 attggctgac atcaagtaat atttgtagtt ttgcccattt acactatcag caaaaacttc 4800 taatttacac tgttatttat gattcaggac ttgctatgga attgatgaag caaggtgcaa 4860 tgagaaccgt attaagtggt cttcttgcag catttgcatg gccagctaca ctgcttgcgg 4920 ctacagactt tattgatagt aaatggtctg ttgctattga caggtaattg caaactctcc 4980 cattctctt atgttgcttg cagagttgca gtgcacactg actatctaca gtattggtat 5040 tcttctgttg atcgacacat aagtttgtgt attcaaattt atgaagatta tgacgtaaca 5100 tttagacaaa cttttaatgt atatatccca cagtacatat attgcagtta aagacatcct 5160 agcaatttgt taacttagcc aatcaaaatc aactgtatct tctcagatca gataaggcag 5220 gaaaaatgct tgctgaagtg cttctcaagg gattgcaagg aaataggtaa ctccatatgc 5280 atttatttca ttagcgatgt tggccgctca gaaatatgcc cccctaatgc caattttttc 5340 ccaggcctgt gactctcatt gggttttcac taggtgcacg tgttatattt aagtgtttac 5400 aggaacttgc tttatcgagt gataatggta cctgttctgt aaccatctcc ttcatccata 5460 agattttcct ttcttggtgg caaattatca gttttaacca tctccatctc ttgtttgcaa 5520 ccagagggac ttgttgagag ggtggttctt cttggtgctc cagtttctgt gaaaggcgag 5580 cggtgggagg ctgcaaggaa ggtaatggcg atgaaatcat ttttttctct attatctctg 5640 taggaatggt gcaacgtaac aacaagaaac agtgaagttg ctgttcattt tatcccagct 5700 tcttccctcg gcgcttaatt cactcacttg tcctcatggc agatggttgc tggaagattt 5760 gtgaatgtgt actccacaga tgactggatt cttggtgtta cttttcgtgc gaggtaaaca 5820 gatttatgat ttcagagctc ctagaaagct catatcacca ttcccccatg tgagctatga 5880 cttaagtaat cttctcgtgc agtctgctaa cacaagggtt ggctggcatc caggccattg 5940 atgtccctgg tgttgaaaat gtaagcatct ggggtgccat tcttgtgttc ttacgtgatt 6000 ttcatctatc ttttacacgt ctttgtgtac aatgcaattt acaggtcgat gttacggagc 6060 ttgtcgatgg ccattcgtct tacctgtcgg cagcacaaca gatactggag catcttgaac 6120 taaataccta ctatccggtt tttgttcccc tgagcgcagc caacgaagaa acagatggaa 6180 cagttgcaca gtaacgtgtg ggcaggattc gatgaaaatc gtatgtagac aaccaaatgt 6240 aacagactat atatacaacc aaatgtaaca gactaactcg ttcaaactgt aaaccagtct 6300 cagcctgagg cgaatgtgtc tggttggtga ggcttgctgt ttctggccgt gtc 6353 <210> 6 <211> 4183 <212> DNA <213> Rice male sterile mutant sts1-1 (Oryza sativa) <400> 6 agtctccccc aaccaaaatt tgaccgagtt tggcacgaaa gaaaccgcct cgacgccgtc 60 caacggaagc cagccgaggc gaccaaccca agggtaggcc taggcgtaaa ccctgcacct 120 gaatcgatcg ccggcgatgg ccacgacgct gacgccgacg cagcgctacg cggcgggggc 180 gctgctggcg ctcgcgctcc gccaggccca gatccaccag tccgtcctcc tcggcgccca 240 ccaccaccac gacgacgacg acgaggaaca ggggcgcacc agcaccagca gcggcggcgg 300 cggcggcagc tcctcctcct cctccaactc cggcgccggc gccgacgccg acctctggac 360 acacgactcc cacggcctcc tccgccccgt cttcaggtgt tttctcgctc ctctccgctt 420 gtaaatttgg ggggtttgag atgaatgctt ccgctttact tggcgatcgg ggggctctgc 480 taggttcctg gagatcgatc ccaaggcgtg gtcggggctg gaggagacgg cggcgtcgtc 540 cgaggccaag catcacatcg gcgcggtacg gcactcactg cattttctgc cctcgtattg 600 taattggccg tgcgttttgg tcagtgttgc agtgatggtt tggggcttag gtgtttgatt 660 atatgcctac aagactgttc aacttcaatt tccgcgtgtg cttgaccagt gaaatttgtg 720 ttttcgtagc atctcatatc actctcattt tgtagtttct aaggataata ttcgaagaag 780 atggcgaaag ctcctcagac agatccgttc aggagcttgc cttggcaaaa ggagttgatg 840 tgatggtaat gagtttgggc aatgacagtg aagtgggtaa cacaattaaa ggtggggatc 900 aagatgcttt gcctagcagc tctggaacag ataaatcccc agggaaaagc tctcatgatg 960 1020 tggcattgct gtttgctctt ctctcggctt gtgttgctga taaacctgta tcaaagagg 1080 aaggaccag aaaatcaacc cgcttcagaa agggtttga tgctcggcat cgtgttgctc 1140 ttcggctgct atctacatgg cttgatgtga agtggatcaa aatggtttgt agagactaaa 1200 1260 agaaaaaaga acccccaatg tttttcatgt ttcaccaaat ttgtcaccaa ccatgctttt 1320 tcgctgcagg aagcaattga ggttatggtt gcatgctctg ccatggcagc agcgaagaa 1380 caagaacaat cacaagaaag cgcatcaccc aaaagtaat gggaaaaatg gaagcgtgga 1440 ggaataattg gtgcagctgc tttgactgga ggagcactgt tggctattac tggggtatg 1500 aaaaaacttc agaaattctg ctggacgctg aacatctgaa ctagtttctg tcgctcatat 1560 tactctaaac ggttgatatt ctcttccaac ttgacaggtt tagctgctcc agcaattgct 1620 gcagggtttg gtgctcttgc tccaaccctg ggcacacttg tccctgttat aggagctagc 1680 ggatttgctg ctatggctac cgctgcagga tctgttgctg gctctgtggc agttgctgca 1740 tcatttggag gtgtgtctac atgttcttca aaacccttct ggtgatgttg ttttgagctt 1800 tcgtttcgtc atgctacatt actatcagct gcagtctccc tttccttttg tcatgagggt 1860 tgcaatattg tttagtgaac ttacccaaga atatgcacac aacactttta ggggtgcatt 1920 ttacaattag ataatagttc gatggattac cagaagaagg ataagactta tacaatgtat 1980 gacccaaaat tgtgagaaac atatggatga aagcgtaatg agaatagttg tggtgtggaa 2040 attttctatt tattcatgct tatcctttga aatgatattt ttcaattagc tgctggagct 2100 ggcttgacgg ggagcaaaat ggccagaaga attgggagcg tgaaagaatt tgagttcaaa 2160 cctattggtg aaaaccataa tcagggtgtg agttcctttt ctctttctga tgtcacaaca 2220 aatttcattc tactagattt ttgttcaccc ttttagtagt caaactgatc tggtctggtt 2280 aaatgttctc aacactttca gcggcttgca gttggcatct taatttccgg atttgctttt 2340 gatgaggatg acttttgtag accttgggaa ggatggcagg ataacctaga gaggtataga 2400 gtggttttt tgacatgttc ttttgctga tcactattct agtactttgt catgtgttac 2460 cagctaagag catccttaaa aataattt caagagttga ataatgtctg gcaggtacat 2520 ccttcaatgg gagtctaagc atataattgc agtgagtaca gcaatacagg attggctgac 2580 atcaaatat atttgtagtt ttgcccattt acactatcag caaaaacttc taatttacac 2640 tgttatttat gattcaggac ttgctatgga attgatgaag caaggtgcaa tgagaaccgt 2700 attaagtggt cttcttgcag catttgcatg gccagctaca ctgcttgcgg ctacagactt 2760 tattgatagt aaatggtctg ttgctattga caggtaattg caaactctcc catttctctt 2820 atgttgcttg cagagttgca gtgcacactg actatctaca gtattggtat tcttctgttg 2880 atcgacacat aagtttgtgt attcaaattt atgaagatta tgacgtaaca tttagacaaa 2940 cttttaatgt atatatcccca cagtacatat attgcagtta aagacatcct agcaatttgt 3000 taacttagcc aatcaaaatc aactgtatct tctcagatca gataaggcag gaaaaatgct 3060 tgctgaagtg cttctcaagg gattgcaagg aaataggtaa ctccatatgc atttatttca 3120 ttagcgatgt tggccgctca gaaatatgcc cccctaatgc caattttttc ccaggcctgt 3180 gactctcatt gggttttcac taggtgcacg tgttatattt aagtgtttac aggaacttgc 3240 tttatcgagt gataatggta cctgttctgt aaccatctcc ttcatccata agattttcct 3300 ttcttggtgg caaattatca gttttaacca tctccatctc ttgtttgcaa ccagagggac 3360 ttgttgagag ggtggttctt cttggtgctc cagtttctgt gaaaggcgag cggtgggagg 3420 ctgcaaggaa ggtaatggcg atgaaatcat ttttttctct attatctctg taggaatggt 3480 gcaacgtaac aacaagaaac agtgaagttg ctgttcattt tatcccagct tcttccctcg 3540 gcgcttaatt cactcacttg tcctcatggc agatggttgc tggaagattt gtgaatgtgt 3600 actccacaga tgactggatt cttggtgtta cttttcgtgc gaggtaaaca gatttatgat 3660 ttcagagctc ctagaaagct catatcacca ttcccccatg tgagctatga cttaagtaat 3720 cttctcgtgc agtctgctaa cacaagggtt ggctggcatc caggccattg atgtccctgg 3780 tgttgaaaat gtaagcatct ggggtgccat tcttgtgttc ttacgtgatt ttcatctatc 3840 ttttacacgt ctttgtgtac aatgcaattt acaggtcgat gttacggagc ttgtcgatgg 3900 ccattcgtct tacctgtcgg cagcacaaca gatactggag catcttgaac taaataccta 3960 ctatccggtt tttgttcccc tgagcgcagc caacgaagaa acagatggaa cagttgcaca 4020 gtaacgtgtg ggcaggattc gatgaaaatc gtatgtagac aaccaaatgt aacagactat 4080 atatacaacc aaatgtaaca gactaactcg ttcaaactgt aaaccagtct cagcctgagg 4140 cgaatgtgtc tggttggtga ggcttgctgt ttctggccgt gtc 4183 <210> 7 <211> 4183 <212> DNA <213> Rice male sterile mutant sts1-2 (Oryza sativa) <400> 7 agtctccccc aaccaaaatt tgaccgagtt tggcacgaaa gaaaccgcct cgacgccgtc 60 caacggaagc cagccgaggc gaccaaccca agggtaggcc taggcgtaaa ccctgcacct 120 gaatcgatcg ccggcgatgg ccacgacgct gacgccgacg cagcgctacg cggcgggggc 180 gctgctggcg ctcgcgctcc gccaggccca gatccaccag tccgtcctcc tcggcgccca 240 ccaccaccac gacgacgacg acgaggaaca ggggcgcacc agcaccagca gcggcggcgg 300 cggcggcagc tcctcctcct cctccaactc cggcgccggc gccgacgccg acctctggac 360 acacgactcc cacggcctcc tccgccccgt cttcaggtgt tttctcgctc ctctccgctt 420 gtaaatttgg ggggtttgag atgaatgctt ccgctttact tggcgatcgg ggggctctgc 480 taggttcctg gagatcgatc ccaaggcgtg gtcggggctg gaggagacgg cggcgtcgtc 540 cgaggccaag catcacatcg gcgcggtacg gcactcactg cattttctgc cctcgtattg 600 taattggccg tgcgttttgg tcagtgttgc agtgatggtt tggggcttag gtgtttgatt 660 atatgcctac aagactgttc aacttcaatt tccgcgtgtg cttgaccagt gaaatttgtg 720 ttttcgtagc atctcatatc actctcattt tgtagtttct aaggataata ttcgaagaag 780 atggcgaaag ctcctcagac agatccgttc aggagcttgc cttggcaaaa ggagttgatg 840 tgatggtaat gagtttgggc aatgacagtg aagtgggtaa cacaattaaa ggtggggatc 900 aagatgcttt gcctagcagc tctggaacag ataaatcccc agggaaaagc tctcatgatg 960 1020 tggcattgct gtttgctctt ctctcggctt gtgttgctga taaacctgta tcaaagagg 1080 aaggaccag aaaatcaacc cgcttcagaa agggtttga tgctcggcat cgtgttgctc 1140 ttcggctgct atctacatgg cttgatgtga agtggatcaa aatggtttgt agagactaaa 1200 1260 agaaaaaaga acccccaatg tttttcatgt ttcaccaaat ttgtcaccaa ccatgctttt 1320 tcgctgcagg aagcaattga ggttatggtt gcatgctctg ccatggcagc agcgaagaa 1380 caagaacaat cacaagaaag cgcatcaccc aaaagtaat gggaaaaatg gaagcgtgga 1440 ggaataattg gtgcagctgc tttgactgga ggagcactgt tggctattac tggggtatg 1500 aaaaaacttc agaaattctg ctggacgctg aacatctgaa ctagtttctg tcgctcatat 1560 tactctaaac ggttgatatt ctcttccaac ttgacaggtt tagctgctcc agcaattgct 1620 gcagggtttg gtgctcttgc tccaaccctg ggcacacttg tccctgttat aggagctagc 1680 ggatttgctg ctatggctac cgctgcagga tctgttgctg gctctgtggc agttgctgca 1740 tcatttggag gtgtgtctac atgttcttca aaacccttct ggtgatgttg ttttgagctt 1800 tcgtttcgtc atgctacatt actatcagct gcagtctccc tttccttttg tcatgagggt 1860 tgcaatattg tttagtgaac ttacccaaga atatgcacac aacactttta ggggtgcatt 1920 ttacaattag ataatagttc gatggattac cagaagaagg ataagactta tacaatgtat 1980 gacccaaaat tgtgagaaac atatggatga aagcgtaatg agaatagttg tggtgtggaa 2040 attttctatt tattcatgct tatcctttga aatgatattt ttcaattagc tgctggagct 2100 ggcttgacgg ggagcaaaat ggccagaaga attgggagcg tgaaagaatt tgagttcaaa 2160 cctattggtg aaaaccataa tcagggtgtg agttcctttt ctctttctga tgtcacaaca 2220 aatttcattc tactagattt ttgttcaccc ttttagtagt caaactgatc tggtctggtt 2280 aaatgttctc aacactttca gcggcttgca gttggcatct taatttccgg atttgctttt 2340 gatgaggatg acttttgtag accttgggaa ggatgacagg ataacctaga gaggtataga 2400 gtggttttt tgacatgttc ttttgctga tcactattct agtactttgt catgtgttac 2460 cagctaagag catccttaaa aataattt caagagttga ataatgtctg gcaggtacat 2520 ccttcaatgg gagtctaagc atataattgc agtgagtaca gcaatacagg attggctgac 2580 atcaagtaat atttgtagtt ttgcccattt acactatcag caaaaacttc taatttacac 2640 tgttatttat gattcaggac ttgctatgga attgatgaag caaggtgcaa tgagaaccgt 2700 attaagtggt cttcttgcag catttgcatg gccagctaca ctgcttgcgg ctacagactt 2760 tattgatagt aaatggtctg ttgctattga caggtaattg caaactctcc catttctctt 2820 atgttgcttg cagagttgca gtgcacactg actatctaca gtattggtat tcttctgttg 2880 atcgacacat aagtttgtgt attcaaattt atgaagatta tgacgtaaca tttagacaaa 2940 cttttaatgt atatatcccca cagtacatat attgcagtta aagacatcct agcaatttgt 3000 taacttagcc aatcaaaatc aactgtatct tctcagatca gataaggcag gaaaaatgct 3060 tgctgaagtg cttctcaagg gattgcaagg aaataggtaa ctccatatgc atttatttca 3120 ttagcgatgt tggccgctca gaaatatgcc cccctaatgc caattttttc ccaggcctgt 3180 gactctcatt gggttttcac taggtgcacg tgttatattt aagtgtttac aggaacttgc 3240 tttatcgagt gataatggta cctgttctgt aaccatctcc ttcatccata agattttcct 3300 ttcttggtgg caaattatca gttttaacca tctccatctc ttgtttgcaa ccagagggac 3360 ttgttgagag ggtggttctt cttggtgctc cagtttctgt gaaaggcgag cggtgggagg 3420 ctgcaaggaa ggtaatggcg atgaaatcat ttttttctct attatctctg taggaatggt 3480 gcaacgtaac aacaagaaac agtgaagttg ctgttcattt tatcccagct tcttccctcg 3540 gcgcttaatt cactcacttg tcctcatggc agatggttgc tggaagattt gtgaatgtgt 3600 actccacaga tgactggatt cttggtgtta cttttcgtgc gaggtaaaca gatttatgat 3660 ttcagagctc ctagaaagct catatcacca ttcccccatg tgagctatga cttaagtaat 3720 cttctcgtgc agtctgctaa cacaagggtt ggctggcatc caggccattg atgtccctgg 3780 tgttgaaaat gtaagcatct ggggtgccat tcttgtgttc ttacgtgatt ttcatctatc 3840 tttgacaaat catcgtagaa ccccatggta aagaacacaa aagtaacaaa aatgatgata 3840 ttttacacgt ctttgtgtac aatgcaattt acaggtcgat gttacggagc ttgtcgatgg 3900 aaaaatgtgt tttgtgtata ttacgttatt tgtccagcta catgccagct tgtcgatgg 3900 ccattcgtct tacctgtcgg cagcacaaca gatactggag catcttgaac taaataccta 3960 ggttagcaga cctgtcggca gcacaacaga tatactggag catcttgaac taaataccta 3960 ctatccggtt tttgttcccc tgagcgcagc caacgaagaa acagatggaa cagttgcaca 4020 ctatccggtt tttgttcccc tgagcgcagc caacgaagaa acagatggaa cagttgcaca 4020 gtaacgtgtg ggcaggattc gatgaaaatc gtatgtagac aaccaaatgt aacagactat 4080 gtaacgtgtg ggcaggattc gatgaaaatc gtatgtagac aaccaaatgt aacagactat 4080 atatacaacc aaatgtaaca gactaactcg ttcaaactgt aaaccagtct cagcctgagg 4140 atatacaacc aaatgtaaca gactaactcg ttcaaactgt aaaccagtct cagcctgagg 4140 cgaatgtgtc tggttggtga ggcttgctgt ttctggccgt gtc 4183 cgaatgtgtc tggttggtga ggcttgctgt ttctggccgt gtc 4183 <210> 8<210> 8 <211> 4184<211> 4184 <212> DNA <212> DNA <213> 水稻雄性不育突变体sts1-3(Oryza sativa) <213> Rice male sterile mutant sts1-3 (Oryza sativa) <400> 8 <400> 8 agtctccccc aaccaaaatt tgaccgagtt tggcacgaaa gaaaccgcct cgacgccgtc 60 agtctccccc aaccaaaatt tgaccgagtt tggcacgaaa gaaaccgcct cgacgccgtc 60 caacggaagc cagccgaggc gaccaaccca agggtaggcc taggcgtaaa ccctgcacct 120 caacggaagc cagccgaggc gaccaaccca agggtaggcc taggcgtaaa ccctgcacct 120 gaatcgatcg ccggcgatgg ccacgacgct gacgccgacg cagcgctacg cggcgggggc 180 gaatcgatcg ccggcgatgg ccacgacgct gacgccgacg cagcgctacg cggcgggggc 180 gctgctggcg ctcgcgctcc gccaggccca gatccaccag tccgtcctcc tcggcgccca 240 ccaccaccac gacgacgacg aggacaca ggggcgcacc agcaccagca gcggcggcgg 300 cggcggcagc tcctcctcct cctccaactc cggcgccggc gccgacgccg acctctggac 360 acacgactcc cacggcctcc tccgccccgt cttcaggtgt tttctcgctc ctctccgctt 420 gtaaatttgg ggggtttgag atgaatgctt ccgctttact tggcgatcgg ggggctctgc 480 taggttcctg gagatcgatc ccaaggcgtg gtcggggctg gaggagacgg cggcgtcgtc 540 cgaggccaag catcacatcg ggcgcggtac ggcactcact gcattttctg ccctcgtatt 600 gtaattggcc gtgcgttttg gtcagtgttg cagtgatggt ttggggctta ggtgtttgat 660 tatatgccta caagactgtt caacttcaat ttccgcgtgt gcttgaccag tgaaatttgt 720 gttttcgtag catctcatat cactctcatt ttgtagtttc tagggataat attcgaagaa 780 gatggcgaaa gctcctcaga cagatccgtt caggagcttg ccttggcaaa aggagttgat 840 gtgatggtaa tgagtttggg caatgacagt gaagtgggat acaaattaa aggtggggat 900 caagatgctt tgcctagcag ctctggaaca gataaatccc caggggaag ctctcatgat gaccagttag gaccata attgacctta gatgatattc ctgctaaca tcaccggaag atggcattgc tgtttgctct tctctcggct tgtgttgctg ataaacctgt atcacaagag gaagaggaca gaaaatcaac ccgcttcaga aagggttatg atgctcggca tcgtgttgct cttcggctgc tatctacatg gcttgatgtg aagtggatca aaatggtttg tacagactaa acttttttgg attcagcaga gtaatcacta tgggtaccaa acaatatcct aaggcaatta aagaaaaaag aacccccaat gtttttcatg tttcaccaaa tttgtcacca accatgcttt ttcgctgcag gaagcaattg aggttatggt tgcatgctct gccatggcag cagcgaaaga acaagaaca tcacaagaa gcgcatcacc caaaagtaa tgggaaaaat ggaagcgtgg aggaataatt ggtgcagctg ctttgactgg aggagcactg ttggctatta ctggggggtat gaaaaaactt cagaaattct gctggacgct gaacatctga actagtttct gtcgctcata ttactctaaa cggttgatat tctcttccaa cttgacaggt ttagctgctc cagcaattgc tgcagggttt ggtgctcttg ctccaaccct gggcacactt gtccctgtta taggagctag 1680 cggattgct gctatgcta ccgctgcagg atctgttgct ggctctgtgg cagttgctgc 1740 atcatttgga ggtgtgtcta catgttcttc aaaacccttc tggtgatgtt gttttgagct 1800 ttcgtttcgt catgctacat tactatcagc tgcagtctcc ctttcctttt gtcatgaggg 1860 ttgcaatatt gtttagtgaa cttacccaag aatatgcaca caacacttttt aggggtgcat 1920 tttacaatta gataatagtt cgatggatta ccagaagaag gataagactt atacaatgta 1980 tgacccaaaa ttgtgagaaa catatggatg aaagcgtaat gagaatagtt gtggtgtgga 2040 aatttcttat ttattcatgc ttatcctttg aaatgatatt tttcaattag ctgctggagc 2100 tggcttgacg gggagcaaaa tggccagaag aattgggagc gtgaaagaat ttgagttcaa 2160 acctattggt gaaaaccata atcagggtgt gagttccttt tctctttctg atgtcacaac 2220 aaatttcatt ctactagatt tttgttcacc cttttagtag tcaaactgat ctggtctggt 2280 taaatgttct caacactttc agcggcttgc agttggcatc ttaatttccg gatttgcttt 2340 tgatgaggat gactttgta gaccttggga aggatggcag gataacctag agaggtatag 2400 agtggtttt ttgacatgtt cttttgctg atcactattc tagtactttg tcatgtgtta 2460 ccagctaaga gcatccttaa aatataatt tcaagagttg aataatgtct ggcaggtaca 2520 tccttcaatg ggagtctaag catataattg cagtgagtac agcaatacag gattggctga 2580 catcaagtaa tatttgtagt tttgcccatt tacactatca gcaaaaactt ctaatttaca 2640 ctgttattta tgattcagga cttgctatgg aattgatgaa gcaaggtgca atgagaaccg 2700 tattaagtgg tcttcttgca gcatttgcat ggccagctac actgcttgcg gctacagact 2760 ttattgatag taaatggtct gttgctattg acaggtaatt gcaaactctc ccatttctct 2820 tatgttgctt gcagagttgc agtgcacact gactatctac agtattggta ttcttctgtt 2880 gatcgacaca taagtttgtg tattcaaatt tatgaagatt atgacgtaac atttagacaa 2940 acttttaatg tatatatccc acagtacata tattgcagtt aaagacatcc tagcaatttg 3000 ttaacttagc caatcaaaat caactgtatc ttctcagatc agataaggca ggaaaaatgc 3060 ttgctgaagt gcttctcaag ggattgcaag gaaataggta actccatatg catttatttc 3120 attagcgatg ttggccgctc agaaatatgc ccccctaatg ccaatttttt cccaggcctg 3180 tgactctcat tgggttttca ctaggtgcac gtgttatatt taagtgttta caggaacttg 3240 ctttatcgag tgataatggt acctgttctg taaccatctc cttcatccat aagattttcc 3300 tttcttggtg gcaaattatc agttttaacc atctccatct cttgtttgca accagaggga 3360 cttgttgaga gggtggttct tcttggtgct ccagtttctg tgaaaggcga gcggtgggag 3420 gctgcaagga aggtaatggc gatgaaatca tttttttctc tattatctct gtaggaatgg 3480 tgcaacgtaa caacaagaaa cagtgaagtt gctgttcatt ttatcccagc ttcttccctc 3540 ggcgcttaat tcactcactt gtcctcatgg cagatggttg ctggaagatt tgtgaatgtg 3600 tactccacag atgactggat tcttggtgtt acttttcgtg cgaggtaaac agatttatga 3660 tttcagagct cctagaaagc tcatatcacc attcccccat gtgagctatg acttaagtaa 3720 tcttctcgtg cagtctgcta acacaagggt tggctggcat ccaggccatt gatgtccctg 3780 gtgttgaaaa tgtaagcatc tggggtgcca ttcttgtgtt cttacgtgat tttcatctat 3840 cttttacacg tctttgtgta caatgcaatt tacaggtcga tgttacggag cttgtcgatg 3900 gccattcgtc ttacctgtcg gcagcacaac agatactgga gcatcttgaa ctaaatacct 3960 actatccggt ttttgttccc ctgagcgcag ccaacgaaga aacagatgga acagttgcac 4020 agtaacgtgt gggcaggatt cgatgaaaat cgtatgtaga caaccaaatg taacagacta 4080 tatatacaac caaatgtaac agactaactc gttcaaactg taaaccagtc tcagcctgag 4140 gcgaatgtgt ctggttggtg aggcttgctg tttctggccg tgtc 4184 <210> 9 <211> 4122 <212> DNA <213> Rice male sterile mutant sts1-4 (Oryza sativa) <400> 9 agtctccccc aaccaaaatt tgaccgagtt tggcacgaaa gaaaccgcct cgacgccgtc 60 caacggaagc cagccgaggc gaccaaccca agggtaggcc taggcgtaaa ccctgcacct 120 gaatcgatcg ccggcgatgg ccacgacgct gacgccgacg cagcgctacg cggcgggggc 180 gctgctggcg ctcgcgctcc gccaggccca gatccaccag tccgtcctcc tcggcgccca 240 ccaccaccac gacgacgacg acgaggaaca ggggcgcacc agcaccagca gcggcggcgg 300 cggcggcagc tcctcctcct cctccaactc cggcgccggc gccgacgccg acctctggac 360 acacgactcc cacggcctcc tccgccccgt cttcaggtgt tttctcgctc ctctccgctt 420 gtaaatttgg ggggtttgag atgaatgctt ccgctttact tggcgatcgg ggggctctgc 480 taggttcctg gagatcgatc ccaaggcgtg gtcggggctg gaggagacgg cggcgtcgtc 540 cgaggccaag catcacatcg gcgcggtacg gcactcactg cattttctgc cctcgtattg 600 taattggccg tgcgttttgg tcagtgttgc agtgatggtt tggggcttag gtgtttgatt 660 atatgcctac aagactgttc aacttcaatt tccgcgtgtg cttgaccagt gaaatttgtg 720 ttttcgtagc atctcatatc actctcattt tgtagtttct aaggataata ttcgaagaag 780 atggcgaaag ctcctcagac agatccgttc aggagcttgc cttggcaaaa ggagttgatg 840 tgatggtaat gagtttgggc aatgacagtg aagtgggtaa cacaattaaa ggtggggatc 900 aagatgcttt gcctagcagc tctggaacag ataaatcccc agggaaaagc tctcatgatg 960 1020 tggcattgct gtttgctctt ctctcggctt gtgttgctga taaacctgta tcaaagagg 1080 aaggaccag aaaatcaacc cgcttcagaa agggtttga tgctcggcat cgtgttgctc 1140 ttcggctgct atctacatgg cttgatgtga agtggatcaa aatggtttgt agagactaaa 1200 1260 agaaaaaaga acccccaatg tttttcatgt ttcaccaaat ttgtcaccaa ccatgctttt 1320 tcgctgcagg aagcaattga ggttatggtt gcatgctctg ccatggcagc agcgaagaa 1380 caagaacaat cacaagaaag cgcatcaccc aaaagtaat gggaaaaatg gaagcgtgga 1440 ggaataattg gtgcagctgc tttgactgga ggagcactgt tggctattac tggggtatg 1500 aaaaaacttc agaaattctg ctggacgctg aacatctgaa ctagtttctg tcgctcatat 1560 tactctaaac ggttgatatt ctcttccaac ttgacaggtt tagctgctcc agcaattgct 1620 gcagggtttg gtgctcttgc tccaaccctg ggcacacttg tccctgttat aggagctagc 1680 ggatttgctg ctatggctac cgctgcagga tctgttgctg gctctgtggc agttgctgca 1740 tcatttggag gtgtgtctac atgttcttca aaacccttct ggtgatgttg ttttgagctt 1800 tcgtttcgtc atgctacatt actatcagct gcagtctccc tttccttttg tcatgagggt 1860 tgcaatattg tttagtgaac ttacccaaga atatgcacac aacactttta ggggtgcatt 1920 ttacaattag ataatagttc gatggattac cagaagaagg ataagactta tacaatgtat 1980 gacccaaaat tgtgagaaac atatggatga aagcgtaatg agaatagttg tggtgtggaa 2040 attttctatt tattcatgct tatcctttga aatgatattt ttcaattagc tgctggagct 2100 ggcttgacgg ggagcaaaat ggccagaaga attgggagcg tgaaagaatt tgagttcaaa 2160 cctattggtg aaaaccataa tcagggtgtg agttcctttt ctctttctga tgtcacaaca 2220 aatttcattc tactagattt ttgttcaccc ttttagtagt caaactgatc tggtctggtt 2280 aaatgttctc aacactttca gcggcttgca gttggcatct taatttccgg atttgctttt 2340 2400 gtggtttttt tgacatgttc tttttgctga tcactattct agtactttgt catgtgttac 2460 cagctaagag catccttaaa aatataattt caagagttga ataatgtctg gcaggtacat 2520 ccttcaatgg gagtctaagc atataattgc agtgagtaca gcaatacagg attggctgac 2580 atcaagtaat atttgtagtt ttgcccattt acactatcag caaaaaacttc taatttacac 2640 tgttatttat gattcaggac ttgctatgga attgaatgaaa aggtgcaatg agaaccgtat 2700 taagtggtct tcttgcagca tttgcatggc cagctacact gcttgcggct acagacttta 2760 ttgatagtaa atggtctgtt gctattgaca ggtaattgca aactctccca tttctctttat 2820 gttgcttgca gagttgcagt gcacactgac tatctacagt attggtattc ttctgttgat 2880 cgacacata gtttgtgtat tcaaatttat gaagattatg acgtaacatt tagacaaact 2940 tttaatgtat atatcccaca gtacatatat tgcagttaaa gacatcctag caatttgtta 3000 acttagccaa tcaaaatcaa ctgtatcttc tcagatcaga taaggcagga aaaatgcttg 3060 ctgaagtgct tctcaaggga ttgcaaggaa ataggtaact ccatatgcat ttattcatt 3120 agcgatgttg gccgctcaga aatatgcccc cctaatgcca attttttcc aggcctgtga 3180 ctctcattgg gttttcacta ggtgcacgtg ttatatttaa gtgtttacag gaacttgctt 3240 tatcgagtga taatggtacc tgttctgtaa ccatctcctt catccataag attttccttt 3300 cttggtggca aattatcagt tttaaccatc tccatctctt gtttgcaacc agagggactt 3360 gttgagaggg tggttcttct tggtgctcca gtttctgtga aaggcgagcg gtgggaggct 3420 gcaaggaagg taatggcgat gaaatcattt ttttctctat tatctctgta ggaatggtgc 3480 aacgtaacaa caagaacag tgaagttgct gttcatttta tcccagcttc ttccctcggc 3540 gcttaattca ctcacttgtc ctcatggcag atggttgctg gaagatttgt gaatgtgtac 3600 tccacagatg actggattct tggtgttact tttcgtgcga ggtaaaaga tttatgattt 3660 cagagctcct agaaagctca tatcaccatt cccccatgtg agctatgact tagtaatct 3720 tctcgtgcag tctgctaaca caagggttgg ctggcatcca ggccattgat gtccctggtg 3780 ttgaaaatgt aagcatctgg ggtgccattc ttgtgttctt acgtgatttt catctatctt 3840 ttacacgtct ttgtgtacaa tgcaatttac aggtcgatgt tacggagctt gtcgatggcc 3900 attcgtctta cctgtcggca gcacaacaga tactggagca tcttgaacta aatacctact 3960 atccggtttt tgttcccctg agcgcagcca acgaagaaac agatggaaca gttgcacagt 4020 aacgtgtggg caggattcga tgaaaatcgt atgtagacaa ccaaatgtaa cagactatat 4080 atacaaccaa atgtaacaga ctaactcgtt caaactgtaa ac 4122 <210> 10 <211> 16 <212> DNA <213> Artificial Sequence <400> 10 ctgtagccgc aagcag 16 <210> 11 <211> 16 <212> DNA <213> Artificial Sequence <400> 11 ttgggagcgt gaaaga 16 <210> 12 <211> 18 <212> DNA <213> Artificial Sequence <400> 12 caaatgctgc aagaagac 18 <210> 13 <211> 20 <212> DNA <213> Artificial Sequence <400> 13 tgaaagcgta atgagaatag 20 <210> 14 <211> 39 <212> DNA <213> STS1 gene (Oryza sativa) <400> 14 aaaaaagcag gctccgaatt catggccacg acgctgacg 39 <210> 15 <211> 46 <212> DNA <213> STS1 gene (Oryza sativa) <400> 15 caagaaagct gggtcgaatt cttactgtgc aactgttcca tctgtt 46 <210> 16 <211> twenty three <212> DNA <213> Artificial Sequence <400> 16 ggccaagcat cacatcggcg cgg 23 <210> 17 <211> twenty three <212> DNA <213> Artificial Sequence <400> 17 gctatggaat tgatgaagca agg 23

Claims

1. A rice male fertility control gene STS1 mutant, characterized in that: Its nucleotide sequence is as shown in SEQ ID NO.6; the mutant is a mutant in which a nucleotide at a splicing recognition site in an intron in the coding region of the STS1 gene is substituted.

2. The biological material related to the rice male fertility control gene STS1 mutant according to claim 1, characterized in that: The biological material is any one of the following 1) to 4): 1) An expression cassette containing the rice male fertility control gene STS1 mutant according to claim 1; 2) A recombinant vector containing the rice male fertility control gene STS1 mutant according to claim 1; 3) A recombinant microorganism containing the rice male fertility control gene STS1 mutant according to claim 1; 4) A recombinant microorganism containing the recombinant vector described in 2).

3. Use of the rice male fertility control gene STS1 mutant according to claim 1 in regulating rice male fertility, wherein the rice male fertility regulation is to obtain male sterile materials.

4. The use according to claim 3, characterized in that: The male sterile material is hybridized and backcrossed with other rice materials respectively to obtain stable genetic male sterile plant lines of different backgrounds that cannot produce normal pollen.

5. The use according to claim 3, characterized in that: The male sterile material is further transformed using genetic engineering technology to form a derived sterile line; the derived sterile line includes the "3G intelligent sterile line" created using the "SPT" technology.

6. The use according to claim 5, characterized in that: The male sterile material or the derived sterile line is used as a female parent of a hybrid rice sterile line in hybrid rice seed production.

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