Apparatus for processing a biological cell culture

Abstract

The invention relates to a treatment apparatus for treating biological cell cultures which are located in storage cavities (13) of carriers (11), having a worktable (3) with one or more support sites (10) for supporting the carriers (11) respectively, wherein the support surface of the support sites (10) corresponds to the base surface of the carriers (11), and having nozzles (4, 5) for generating an air flow in the space (2) above the worktable. In order to avoid the transport of droplets or cell material from one storage cavity to another storage cavity by means of a horizontal air flow above the carriers during the treatment, the nozzles (4, 5) are arranged outside the support surface (10) and along the edge of the support surface, or arranged within the support surface (10) and generate an air curtain which surrounds the carriers (11) or the storage cavities (13).

Description

Technical Field

[0001] The present invention relates to an apparatus for processing biological cell cultures located in a storage cavity of a carrier, the processing apparatus having a worktable having one or more support positions for supporting the carrier, wherein the support surface of the support position corresponds to the base surface of the carrier, and the apparatus having nozzles for generating airflow in the space above the worktable. Background Technology

[0002] Processing devices for handling biological cells are known in the prior art, particularly from documents US 2018 / 0202908 A1, US 6,146,592, EP 3229028 A1, US 2014 / 0273242 A1, WO 03 / 008103 A1, WO93 / 03891 A1, EP 2068155 A2, WO 2011 / 047710 A1, US 2007 / 005920 A1, and US 6,360,792B1. This type of processing device has a housing that is substantially airtight to the outside. Inside the housing is a worktable, such as a workbench, with multiple support positions on which carriers can be placed. The carriers are preferably standardized microplates, such as those described in US 2012 / 0178120 A1 or US 6,143,250. Cell cultures can be removed from the storage chamber of the carrier using pipetting devices. Nutrients can also be supplied to the storage chamber using pipetting devices. Nutrients can be stored in nutrient containers. These containers can have a planar profile conforming to the planar contours of the carrier. Within the housing of the processing equipment, a pure airflow is generated by a fan and a filter, flowing in a laminar flow parallel to the worktable. During the processing of cell cultures stored in the storage chambers, liquids are transported. Droplets, particularly microdroplets, may form, carried by the airflow. This can result in cells from cell cultures arranged in the storage chambers being transported to adjacent storage chambers containing cells from other cell cultures. Such unintentional contamination by foreign cell cultures is undesirable.

[0003] DE 10303736 B4 describes an air-conditioned cabinet with a loading opening through which glass slides can be transported. The loading opening has two sidewalls facing each other, with openings in the sidewalls through which airflow can be generated into the loading opening.

[0004] DE 3879631 T2 describes a carrier for cell cultures having a space that can be flushed with gas and a gas outlet disposed between adjacent storage chambers of the carrier.

[0005] US 5,525,512 discloses a cabinet for containing carriers, having a storage cavity for containing cell cultures, wherein openings are provided next to the support for containing the carriers.

[0006] US 4,968,625 describes a carrier having a storage cavity for containing cell cultures, wherein perforations for separating adjacent storage cavities are arranged between the storage cavities.

[0007] WO 2007 / 043561 A1 describes a table for holding a carrier containing cell cultures, wherein the table has an opening through which light passes, allowing the cell cultures to be observed under a microscope. Summary of the Invention

[0008] The technical problem to be solved by the present invention is to provide measures to at least reduce, and preferably prevent, unintentional infection by foreign cell cultures. The present invention particularly relates to improvements in workbenches and carriers, wherein the carrier is preferably a plate-like carrier in the form of microplates and has multiple storage cavities.

[0009] The technical problem is solved by the invention given in the claims, wherein the dependent claims are not only advantageous extensions of the invention given in the parallel claims, but also independent solutions to the technical problem.

[0010] First, the present invention relates to a processing apparatus for processing biological cell cultures located in storage cavities of one or more carriers. A worktable is provided having one or more support positions for supporting the carriers. The support surface of each support position has a base surface corresponding to the base surface of the carrier. Particularly, devices are provided that allow the carriers on the support surfaces to be adjusted. In this case, the carriers occupy a predetermined position on the worktable. According to the invention, nozzles are arranged outside the support surface and along its edge. Airflow can be generated through these nozzles, the direction of which is transverse to the planar extension direction of the support surface or transverse to the planar extension direction of the upper side of the worktable. The airflow generated by the nozzles preferably extends transversely to a laminar flow parallel to the worktable within the housing. An air curtain is generated by the airflow generated by the nozzles, which can surround the carrier and form a barrier preventing droplets from being carried away from one carrier, and particularly preventing them from being transported to other carriers. In an alternative embodiment of the invention, the nozzles are arranged within the support surface. The carrier used in this alternative embodiment has one or more storage cavities for containing biological cell cultures, each having a planar profile or an open surface. One or more vents are arranged outside the planar profile or opening surface and along the edge of the planar profile or opening surface. The vents may be arranged annularly around the planar profile or opening surface of the storage cavity. The vents form openings on the underside of the carrier, aligned with nozzles, particularly arranged within the support surface of the worktable, such that airflow generated by the nozzles can pass through the vents. This alternative creates an air curtain surrounding the storage cavity, thus providing a barrier that prevents droplets from being transported from one storage cavity, particularly towards adjacent storage cavities. The extended designs mentioned below constitute, both individually and in combination, an extended design of the device according to the invention or of the carrier according to the invention. The support surface is rectangular. However, the support surface may also have a different planar profile, such as a polygonal or circular surface. The nozzles extend along lines that extend at a constant distance relative to the edge of the support surface. These lines may be straight lines extending parallel to the edge of the support surface. However, these lines may also be curved. The worktable may have a circular shape. Multiple support surfaces are arranged around the center of the worktable. These support surfaces may have the same radial distance relative to the center. The worktable is capable of rotating around the center. Devices can be provided for generating airflow within an outwardly substantially or nearly airtight housing, the airflow being conveyed through the housing and delivered above a worktable arranged within the housing. These devices may include filters and fans. The airflow generated by the fan, preferably in a stratified manner, has a direction perpendicular to the airflow generated by the nozzles. Vents within the carrier form a connecting channel between a lower and upper side of the carrier, the lower side preferably planarly supported on a support surface, with the opening of the storage cavity located within the upper side. Multiple nozzles may be interconnected with gas lines. The gas lines may be located within the worktable. A pump is connected to the gas lines to generate negative pressure.In this configuration, the nozzle functions as an intake nozzle and generates airflow into the worktable. However, the airflow can also exit from the nozzle into the housing, generated by a second fan. For adjustment at the support position, a carrier preferably composed of a carrier plate, and particularly preferably of a microplate having a planar profile of 127.76 mm × 85.48 mm, can be provided with positioning sides. The carrier can have positioning sides that abut against the positioning sides of the support position. Positioning sides can be formed by the sidewalls of recesses or by the sidewalls of ribs or connecting plates. The nozzles are preferably arranged close to the positioning sides. Here, the positioning sides of the support position can form a frame or a segment forming a frame. In this case, the nozzles are preferably close to the frame. These nozzles are preferably arranged to create an air curtain that completely surrounds the carrier or storage cavity and / or the same. The nozzles can be formed by elongated slits extending in the upper side of the worktable. The openings of the vents leading to the upper side of the carrier are also preferably elongated. The openings of the nozzles or vents are so closely adjacent that a closed air curtain is created. The storage cavity can preferably have a circular shape. The vent can extend along an arc around the opening of the storage cavity. In a variation of the invention, the storage cavity has a tubular wall. This wall has an inner side and an outer side. The inner side is the inner wall of the storage cavity. The outer side is adjacent to the vent, thereby generating an airflow that sweeps along the outer side of the wall. In an extended design of the invention, it is suggested that the carrier having the storage cavity is a first carrier, and preferably a second carrier having the same basal surface as the first carrier carries the liquid or other treatment agent. The second carrier particularly has a storage volume for nutrient solution or a support for holding pipette tips. Generating directional airflow through openings that are substantially known in the prior art is considered an advantage of the invention. It is particularly advantageous here that these directional airflows extend transversely to the laminar airflow. Furthermore, it is advantageous that the actively generated airflow forms an air curtain. Attached Figure Description

[0011] Embodiments of the present invention are illustrated below with reference to the accompanying drawings. In the drawings:

[0012] Figure 1 A schematic cross-sectional view of the device according to the invention is shown;

[0013] Figure 2 A top view of the worktable 3 with multiple support positions 10 according to line II-II is shown;

[0014] Figure 3 Show Figure 2 Enlarged screenshot III;

[0015] Figure 4 A perspective view of the support position 10 on the worktable 3 is shown;

[0016] Figure 5 The different designs of the support positions 10 on the worktable 3 are also shown in perspective view.

[0017] Figure 6 A top view of a carrier 11 having multiple storage cavities 13 is shown;

[0018] Figure 7 Showing according to Figure 6 A cross-sectional view of line VII-VII in the middle;

[0019] Figure 8 Shown in sectional view according to Figure 6 and Figure 7 The carrier is installed into the groove formed by the positioning side 14 of the support position 10 of the worktable 3; and

[0020] Figure 9 A perspective view showing another embodiment of the carrier 11. Detailed Implementation

[0021] Figure 1 A housing 1 is shown, and a processing chamber 2 arranged within the housing 1 is shielded from the ambient atmosphere by the housing. Airflow is generated by a fan 21 and filtered by a filter 20. The airflow generated by the fan 21 enters the processing chamber 2 through an inlet 19, flows generally horizontally through the inlet, and exits the processing chamber through an outlet 23.

[0022] A worktable 3 with a circular upper side exists within the processing chamber 2. The worktable 3 has a center Z and can be rotated around the center Z to different working positions. Multiple processing agents can be placed within the processing chamber 2. A pipetting device 8 is shown as an example. Using this pipetting device 8, liquid can be taken from a bottle 24 arranged on a carrier 12 through a pipette. This liquid can be transferred to a storage chamber 13 of another carrier 11. Cell cultures are present in the storage chamber 13.

[0023] Multiple support positions 10 are arranged on the upward-pointing wide side of the worktable 3. Figure 4 and Figure 5 Different variations of the support 10 are illustrated exemplaryly. Positioning sides 6 are provided, through which a carrier 11 having multiple storage cavities 13 or a carrier 12 carrying liquids or treatment agents can be reproducibly positioned on the support 10. The carrier 11 preferably has a planar profile of a microplate.

[0024] Support 10 defines a support surface that corresponds to the base surface of carriers 11 and 12. The support surface has an edge. A plurality of nozzles 4 extend beyond this edge, and these nozzles are capable of generating an airflow that flows transversely to the aforementioned horizontal airflow. The nozzles 4 thus generate a vertical airflow. According to the invention, these nozzles are suction nozzles, but could also be blowing nozzles. Nozzles 5 are connected to a gas conduit 9 extending inside or below the worktable 3. The gas conduit 9 connects nozzles 5 to a pump 22 that generates negative pressure, thereby allowing gas from the horizontal airflow to be drawn out through the nozzles 4.

[0025] Nozzles 4 are designed as elongated slit nozzles with a constant spacing relative to the edge of support 10. These nozzles are arranged so closely together that they create a substantially closed air curtain around support 10, which extends along the edge of support 10.

[0026] exist Figure 4 In the illustrated embodiment, the support 10 is formed by a groove, wherein the sidewalls of the groove form positioning sides 6, and the edge surfaces of the carriers 11 and 12 can abut against these positioning sides. The nozzle 4 can be directly adjacent to the sidewalls of the carriers 11 and 12.

[0027] exist Figure 5 In the illustrated embodiment, the positioning side 6 is formed by a mounting plate 7. The mounting plate can be an angular mounting plate 7, which can surround the corner region of the carrier 11. A section of the sidewall of the carrier 11 can abut against the inner side of the mounting plate 7. The nozzle 4 can be directly adjacent to the outer wall of the mounting plate 7.

[0028] Figure 4 and Figure 5 Not only is the nozzle 4 arranged outside the support surface of the support position 10 shown, but also the nozzle 5 arranged inside the support surface is shown. The nozzle 5 is also connected to the gas line 9, so that the nozzle 5 can be used as a blowing nozzle or a suction nozzle, wherein the nozzle 5 is preferably used as a suction nozzle.

[0029] Figures 6 to 9 Carriers are shown, which have a plate shape and, more particularly, a microplate shape, wherein the microplate has the aforementioned planar profile dimensions. Carrier 11 has a lower side 16 supported on a support surface of support 10. Carrier 11 has an upper side 18 facing away from the lower side 16, in which the opening of a basin-shaped storage cavity 13 extends. The storage cavity 13 may be open, but it may also be closed with a closure or a lid. A vent 15 is arranged along the edge of the opening of the storage cavity 13. The vent 15 forms a flow channel between the upper side 18 and the lower side 16.

[0030] The nozzles 5 are arranged within the support surface of the support position 10 such that the nozzles 5 are flush with the vent 15. The negative pressure generated by the nozzles 5 thus creates an airflow from the processing chamber 2 into the vent 15 and thus creates an air curtain around the storage chamber 13. The nozzles 5 are so closely adjacent that a continuous air curtain is created around the storage chamber 13.

[0031] exist Figure 9 In the illustrated embodiment, the boundary walls of the storage cavity 13 are formed by tubular structures. These tubular structures form a wall 17 having an inner side 17' and an outer side 17"". Vents 15 are arranged in the base plane such that these vents are directly adjacent to the outer side 17"". The formed air curtain sweeps along the outer side 17"".

[0032] An air curtain is created by a horizontally flowing airflow, which protects the upwardly open storage chamber 13 during processing, for example, using a pipette from pipetting device 8. A vertical air curtain is created by an airflow perpendicular to the horizontal air curtain, generated by nozzles 4 and 5. This air curtain prevents aerosols generated during processing, such as during pipetting, from being carried away by the horizontal airflow, for example, and transported to other storage chambers. This prevents cross-contamination of different cell cultures located in different storage chambers 13. The arrangement of nozzles 4 and 5 or vents 15 is chosen to create an opening pattern showing openings arranged closely in sequence along a line. A continuous airflow flows through these openings.

[0033] Other devices for processing cell cultures, such as devices for opening bottles or microscopes, can be installed in processing chamber 2.

[0034] The foregoing embodiments are used to illustrate the invention as generally included in this application, which extends the prior art through at least the following combinations of features, each of which independently extends the prior art. Two, more, or all of these combinations of features may also be combined with each other, namely:

[0035] A processing device, characterized in that nozzles 4 and 5 are arranged outside the support surface 10 and along the edge of the support surface, or are arranged inside the support surface 10.

[0036] A processing device is characterized in that the support surface 10 is a rectangular surface, and the nozzles 4 and 5 are located on a line extending along and parallel to the edge of the rectangular surface, and / or the worktable 3 has a circular shape and a plurality of support surfaces 10 are arranged around the center Z of the worktable 3, and / or the worktable 3 is rotatable around the center Z, and / or the worktable 3 is arranged in an outwardly airtight housing 1 through which an airflow 1 can be conveyed, and / or devices 20 and 21 are provided to generate a filtered first airflow that flows parallel to the worktable 3 within the housing, wherein a second airflow generated by the nozzles 4 and 5 extends perpendicular to the first airflow.

[0037] A processing apparatus characterized in that one or more carriers 11 have one or more storage cavities 13 for containing biological cell cultures, wherein the carrier 11 has vents 15 disposed outside the planar profile of the storage cavity 13 and along the edge of the planar profile, the vents being flush with nozzles 5 when the carrier 11 is placed on a support 10.

[0038] A carrier characterized in that a vent 15 is arranged outside the planar outline of the storage cavity 13 and along the edge of the planar outline.

[0039] A processing device or carrier, characterized in that the carriers 11, 12 have a rectangular planar profile having a lower side 16 extending in a plane and an upper side 18 extending in a plane parallel to the plane, the opening of the storage cavity 13 being located in the upper side 18, wherein the vent 15 forms a connecting channel between the lower side 16 and the upper side 18, and / or a pump 22 is provided to generate negative pressure in the nozzles 4, 5 or in the gas pipeline 9 connecting the plurality of nozzles 4, 5 to each other.

[0040] A processing device or carrier, characterized in that the support has a first positioning side 6 for abutting against a second positioning side 14 of the carrier 11, and / or the support 10 forms a frame for adjusting the position of the carriers 11 and 12 on the worktable 3, wherein the nozzle 4 is adjacent to the frame or a section of the frame.

[0041] A processing device or carrier is characterized in that gas conduits 9 are arranged below the upper side of the workbench 3 constituting the support 10, and these gas conduits are flow-connected to the nozzles 4, 5, wherein the nozzles 4, 5 are either exhaust nozzles for generating airflow discharged from the workbench 3 or intake nozzles for generating airflow into the workbench 3, and / or the airflow generated by the nozzles 4, 5 constitutes a barrier in the form of an air curtain between adjacent carriers 11, 12 and / or adjacent storage cavities 13, by which the barrier prevents liquid or cells from being transferred from one storage cavity 13 to another storage cavity 13 during the processing of biological cells, and / or the nozzles 4, 5 generate an air curtain of the same shape that completely surrounds the carriers 11, 12 or storage cavities 13.

[0042] A processing device or carrier, characterized in that the planar profile of the storage cavity 13 is circular, and the vent 15 is arranged on an arc line surrounding the planar profile, wherein the support surface 10 has a nozzle 5 arranged on the arc line, and / or the storage cavity 13 has a wall 17 having an inner side 17' pointing towards the storage cavity and an outer side 17" pointing away from the storage cavity, wherein the vent 15 is adjacent to the outer side 17".

[0043] A processing device or carrier, characterized in that: a first carrier 11 is a carrier plate, and / or the first carrier 11 is a microplate having a planar profile of 127.76 mm × 85.48 mm, and / or the storage cavity 13 is a recess, and / or the storage cavity 13 is open or closed upwards, and / or the first carrier 11 has a plurality of storage cavities 13, which are respectively arranged around a plurality of vents 15, and / or a second carrier 12 is provided, the planar profile of the second carrier being the same as that of the first carrier 11, and the second carrier stores processing liquid or processing agent, and / or the nozzles 4, 5 or vents 15 have an elongated planar profile.

[0044] A method characterized by generating an air curtain surrounding a carrier 11 and / or at least one storage cavity 13 via nozzles 4 and 5.

[0045] A method characterized by a first airflow flowing parallel to a worktable 3, the first airflow extending perpendicularly to a second airflow generated by nozzles 4 and 5.

[0046] All disclosed features (either individually or in different combinations) are essential to the invention. Therefore, the disclosure of this application also includes the full content of the relevant / attached priority documents (copies of earlier applications), and for this purpose, the features of the priority documents are also incorporated into the claims of this application. Dependent claims, by their features, characterize unique inventive improvements to the prior art even when they do not possess the technical features of the cited claims, and are particularly useful for divisional applications based on such technical features. The invention given in each claim may additionally have one or more features that are particularly provided with reference numerals in the foregoing description and / or given in the list of reference numerals. The invention also relates to various design options in which certain features mentioned in the foregoing description are not implemented, especially when they are considered irrelevant to the corresponding purpose of use or can be replaced by other means with the same technical effect.

[0047] List of reference numerals

[0048] 1. Shell

[0049] 2 Space

[0050] 3 Workbench

[0051] 4 nozzles

[0052] 5 nozzles

[0053] 6 First positioning side

[0054] 7 Connecting board

[0055] 8. Pipettes

[0056] 9. Gas Piping

[0057] 10 Support position / support surface

[0058] 11. Carrier

[0059] 12 carriers

[0060] 13 Storage cavity

[0061] 14 Second positioning side

[0062] 15 Vents

[0063] 16. Lower side

[0064] 17 walls

[0065] 17' Inner side

[0066] 17" outer side

[0067] 18. Upper side

[0068] 19. Entrance

[0069] 20 Devices, Filters

[0070] 21 components, fan

[0071] 22 pumps

[0072] 23 Discharge outlet

[0073] 24 bottles

[0074] Z Center

Claims

1. A processing apparatus for processing biological cell cultures, the cell cultures being located in a storage cavity (13) of a carrier (11), the processing apparatus having a worktable (3) having one or more support positions (10) for supporting the carrier (11), each support position having a support surface, wherein, The support (10) is provided with a plurality of openings (4) through which airflow passes. These openings are suction nozzles or blowing nozzles connected to a plurality of gas lines (9) for generating airflow oriented transversely to the support surface. The support (10) is characterized in that each support has a support surface defined by positioning sides (6, 14) corresponding to the base surface of the carrier (11). The plurality of openings (4) arranged outside the support surface and along the edge of the support surface are arranged so closely adjacently that these openings generate closed air curtains around the support (10) that extend along the edge of the support (10).

2. A processing apparatus for processing biological cell cultures, the cell cultures being located in a storage cavity (13) of a carrier (11), the processing apparatus having a worktable (3), the worktable having one or more support positions (10) for supporting the carrier (11), each support position having a support surface, wherein, The device is provided with a plurality of openings (5) through which airflow passes. These openings are suction nozzles or blowing nozzles connected to a plurality of gas lines (9) for generating airflow oriented transversely to the support surface. The device is characterized in that the openings (5) are arranged inside the support position (10), wherein the openings (5) are arranged so closely adjacent to each other that a continuous air curtain is generated around the storage cavity (13).

3. The processing apparatus according to claim 1 or 2, characterized in that, The gas lines (9) of these suction nozzles (4, 5) are connected to the pump (22) that generates negative pressure.

4. The processing apparatus according to claim 1, characterized in that, The openings (4) are designed as elongated slit nozzles with a constant spacing relative to the edge of the support (10), where they are arranged so closely adjacently that they create a closed air curtain around the support (10) that the air curtain extends along the edge of the support (10).

5. The processing apparatus according to claim 2, characterized in that, One or more carriers (11) have one or more storage cavities (13) for containing biological cell cultures, wherein the carrier (11) has a plurality of vents (15) that are flush with nozzles (5) when the carrier (11) is placed on a support (10).

6. The processing apparatus according to claim 1 or 2, characterized in that, The device (20, 21) is provided, through which a first airflow is generated within the housing parallel to the worktable (3), wherein a second airflow generated by these openings (4, 5) extends perpendicular to the first airflow.

7. The processing apparatus according to claim 1, characterized in that, The support (10) has a first positioning side (6) that abuts against the second positioning side (14) of the carrier (11).

8. The processing apparatus according to claim 7, characterized in that, The first positioning side (6) is formed by a frame through which the position of the carrier (11) on the workbench (3) can be adjusted, wherein the opening (4) is adjacent to the frame, or the opening and the frame have segments spaced apart from each other, and the opening (4) is adjacent to these segments.

9. The processing apparatus according to claim 1 or 2, characterized in that, These gas pipes (9) are arranged below the upper side of the workbench (3) that forms the support position (10), and these gas pipes are flow-connected to the openings (4, 5), wherein these openings (4, 5) are either exhaust nozzles for generating airflow discharged from the workbench (3) or intake nozzles for generating airflow into the workbench (3).

10. The processing apparatus according to claim 1 or 2, characterized in that, The worktable (3) has a circular shape and multiple support surfaces are arranged around the center (Z) of the worktable (3), and the worktable (3) is rotatable around the center (Z), and the worktable (3) is arranged in an outwardly airtight shell (1).

11. A carrier for use in a processing apparatus according to any one of claims 1 to 10, said carrier having one or more storage cavities (13) for containing biological cell cultures, wherein, The carrier (11) has an upper side (18) extending in a plane, the opening of the storage cavity (13) is located in the upper side (18), and one or more vents (15) are arranged outside the opening, the vents extending along the edge of the storage cavity (13), characterized in that the vents (15) form a connecting channel between the lower side (16) and the upper side (18).

12. The carrier according to claim 11, characterized in that, The storage cavity (13) has a circular planar profile, and the vent (15) is arranged on an arc line surrounding the planar profile.

13. The carrier according to claim 11, characterized in that, The storage cavity (13) has a wall (17) having an inner side (17') pointing towards the storage cavity and an outer side (17") pointing away from the storage cavity, wherein the vent (15) is adjacent to the outer side (17").

14. A method for processing a biological cell culture, said biological cell culture being located in a plurality of storage cavities (13) of a carrier (11) according to claim 11, said carrier being located on a support (10) extending on a support surface of a worktable (3), wherein, Airflow is generated through openings (4, 5), characterized in that an air curtain is generated around the carrier (11) and / or at least one storage cavity (13) through the openings (4, 5), and the air curtain is oriented transversely to the surface extension direction of the support surface.

15. The method according to claim 14, characterized in that, The airflow generated by the openings (4, 5) is configured as a barrier of air curtain between adjacent carriers (11) and / or adjacent storage cavities (13), through which liquid or cells are prevented from being transferred from one storage cavity (13) to another storage cavity (13) during the processing of biological cells.

16. The method according to claim 14, characterized in that, A first airflow is generated parallel to the worktable (3), and a second airflow generated by the openings (4, 5) flows perpendicular to the first airflow.

17. The method according to claim 14, characterized in that, The airflow generated by the openings (4, 5) is either an intake airflow or a blow airflow.

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