A breath-removing composition containing biological zinc and its application in oral care products

Abstract

The invention discloses a breath-removing composition containing bio-zinc and its application in oral care products, belonging to the field of oral care technology. The breath-removing composition includes bio-zinc-yeast / zinc fermentation products, and the content of the bio-zinc in the oral care products is 0.1%-10%. The yeast / zinc fermentation product is applied for the first time in an oral care product with breath-removing function, and while adsorbing sulfides in the breath, the problems of low solubility of conventional organic zinc and strong astringency of inorganic zinc ions are solved. The obtained product can suppress sulfides in the breath for a long time, freshen the breath, and has no obvious astringency or astringency of the oral mucosa.

Description

Technical Field

[0001] The invention relates to the technical field of oral care, and in particular to a breath-removing composition containing biological zinc and application thereof in oral care products. Background Art

[0002] Bad breath, also known as halitosis, refers to a symptom of odor when breathing or opening the mouth. Halitosis is quite common among adults, and epidemiological surveys show that the incidence of halitosis is relatively high. Halitosis is divided into true halitosis (Genuine Halitosis), false halitosis (Pseudo Halitosis) and halitophobia (Halitophobia). True halitosis refers to the obvious bad smell from the mouth that can be felt by others. True halitosis is divided into physiological halitosis (Physiologic Halitosis) and pathological halitosis (Pathologic Halitosis). Physiologic halitosis is caused by the decay process of the oral cavity, and there is no special disease or pathological condition that can cause halitosis. The main source of halitosis is the back of the tongue. Temporary halitosis caused by food factors (garlic, onion, etc.) also belongs to this category. Pathological halitosis is divided into oral halitosis and non-oral halitosis. Oral halitosis is caused by poor oral hygiene or certain oral diseases, such as periodontal disease, unhealed caries, dry mouth, etc. Non-oral halitosis is halitosis caused by diseases of the nasal cavity, throat, lungs or upper digestive system, such as uremia, liver cirrhosis, etc. Pseudo-halitosis refers to the patient's own feeling of having bad breath, and the test result is negative. This symptom can be improved through explanation and psychological counseling. Ostophobia refers to the patient's insistence on having bad breath after treatment of true or false halitosis, and no factors that can cause bad breath are found after examination.

[0003] The causes of halitosis include oral factors, extraoral factors and mental factors. Among the three factors, oral factors are the main pathogenic factors. Studies have shown that 87% of halitosis patients have oral factors (Delanghe G, Ghyselen J, Bollen C, et al. An inventory of patients' response to treatment at a multidisciplinary breath odor clinic [J]. Quintessence Int, 1999, 30: 307-310.). In addition, some mental illnesses and systemic diseases can also cause halitosis.

[0004] According to statistics, 80%-90% of bad breath originates from the oral cavity, such as dental caries, periodontal disease, tongue coating, food impaction, poor restorations, oral mucosal diseases, etc., among which dental caries and periodontal disease are the most common related diseases. The oral cavity contains a large number of microorganisms, which use the amino acids, proteins and other food residues remaining in the oral cavity to decompose and metabolize to produce unpleasant odors - volatile sulfur compounds (VSCs). The main components of sulfides are hydrogen sulfide, methyl mercaptan and ethyl sulfide (Scully C, el-Maaytah M, Porter SR, et al. Breath odor: etippatheogenesis, assessment and management[J]. Eur J Oral Sci, 1997, 105:287-293). Clinical experiments have shown that the level of VSCs is positively correlated with the degree of bad breath (Nakano Y, Yoshimura M, Koga T. Correlation between oral malodor and periodontal bacteria [J]. Microbes Infect, 2002, 4 (6): 67).

[0005] At present, most studies believe that the microorganisms that cause halitosis in the oral cavity (mainly Gram-negative anaerobic bacteria) are mainly concentrated in the gingival sulcus (periodontal pocket) and tongue coating (Tonzetich J, McBride B C. Characterization of volatile sulphur production by pathogenic and non-pathogenic strains of oral Bacteroides[J]. Arch Oral Biol, 1981, 26: 963–969). This is consistent with the research report (Liu Binghong. Classification of halitosis and analysis of related causes[J]. Continuing Medical Education, 2007, 34: 72-74) that the oral sources of VSCs are mainly gingival sulcus (periodontal pocket) and tongue dorsum.

[0006] Patent "Zinc citrate spheres in oral composition" (ZL 03809346) uses zinc citrate as an anti-tartar substance added to the oral composition, and the amount of zinc salt added is 0.001%-20%. Patent "A high-efficiency stain-removing and whitening toothpaste and its manufacturing method" (ZL 200510026049.5) mentions a whitening toothpaste ratio, zinc citrate is 0.5%-3.5%, this patent believes that zinc citrate can dissolve calculus and inhibit plaque calcification deposition, thereby preventing the formation of plaque calculus. Patent "Toothpaste containing calcium carbonate and zinc citrate" (ZL 200580026141.X) mentions a toothpaste composition containing zinc salt, the zinc salt exists in the form of zinc citrate soluble in water, and zinc citrate is used as an anti-calculus agent. The patent "Method for producing attapulgite double fluoride toothpaste" (ZL 200810018916.4) describes attapulgite double fluoride toothpaste as containing zinc citrate, with an addition amount of 0.05%-1.0%. The role of zinc citrate is to prevent the formation of tartar and inhibit the attachment of pigments such as smoke stains and tea stains on the tooth surface. The patent "Method for producing attapulgite toothpaste for removing smoke stains" (ZL 200810018908.X) mentions that the addition amount and role of zinc citrate are the same as those in the previous patent. The patent "Medicinal toothpaste for treating oral diseases and its preparation method" (ZL 201010135299.3) describes that the toothpaste contains zinc citrate, with an addition amount of 0.5%-2%, but does not mention its role. The patent "A toothpaste for removing bad breath and preventing oral ulcers" (ZL 20101047150.7) contains zinc citrate, with an addition amount of 1%-2%. This invention believes that adding naringin, zinc citrate, eucalyptol and other ingredients to toothpaste can remove bad breath and kill harmful oral bacteria. Zinc citrate has the effect of removing bad breath, preventing gingivitis, dental calculus and dental bleeding. The patent "Toothpaste containing stannous fluoride plus zinc citrate and low-level water" (ZL 200980162304.5) contains a zinc ion source, including zinc salts of organic acids and zinc citrate. The patent "Oral Care Composition" (ZL 201180018204.2) contains a citrate ion source that is a slightly water-soluble citrate, and the citrate is zinc citrate. The citrate ion source accounts for at least 0.6% of the total amount of the formula. The reason for choosing zinc citrate is to provide an additional opacifying effect to further improve the aesthetics of the product. This patent believes that zinc ions are also valuable for the anti-plaque and anti-tartar properties of this composition. The composition described in the patent "Oral Care Composition" (ZL 201180018162.2) contains one or more zinc ion sources, which are zinc citrate, which helps to increase the aesthetics and stability of the product. The antimicrobial agent of the composition described in the patent "Oral Care Composition Containing Quinone and Other Antimicrobial Agents" (ZL 201080070593.9) contains zinc-containing compounds, and zinc citrate is one of them.The composition described in the patent "Taste-Masking Oral Care Composition" (ZL 201180067627.3) contains a series of flavoring ingredients for masking oral odor, including metal salts, which are zinc citrate. Zinc citrate has been reported to be used as a sulfide adsorbent in the oral cavity, but due to its low solubility, it is difficult to reach an effective concentration, and a certain concentration of zinc ions will bring obvious astringency and astringency in the oral cavity, limiting its practical application in oral care products.

[0007] Currently, yeast / zinc fermentation products are mainly used as cosmetics, and there has been no research on their use as breath removers. Summary of the invention

[0008] In view of the defects in the prior art, the present invention provides a breath-removing composition containing biological zinc and its application in oral care products. Yeast / zinc fermentation products are applied for the first time in oral care products with breath-removing function. While absorbing sulfides in breath, the problems of low solubility of conventional organic zinc and strong astringency of inorganic zinc ions are solved.

[0009] The breath removing composition containing biological zinc provided by the present invention comprises biological zinc, and its content in oral care products is 0.1%-10% by weight; the biological zinc is yeast / fermentation product.

[0010] The yeast / zinc fermentation product of the combination is prepared by fermentation of brewer's yeast. During the yeast fermentation process, zinc exists in a chelated form with highly active small molecules such as amino acids and polypeptides, so that inorganic zinc is converted into organic zinc, reducing the skin irritation peculiar to inorganic zinc, and is more suitable for oral care product application.

[0011] Preferably, it also includes organic zinc or inorganic zinc, including organic zinc or inorganic zinc, and the weight ratio of the biological zinc to the organic zinc / inorganic zinc is 100:1-5:1; the organic zinc includes any one or more of zinc citrate, zinc acetate, and zinc gluconate, and the inorganic zinc includes any one or more of zinc chloride or zinc sulfate.

[0012] Preferably, the weight ratio of the bio-zinc to the organic zinc / inorganic zinc is 10:1.

[0013] Zinc citrate (organic zinc) in the composition can be used as an antibacterial substance on the one hand, which can effectively inhibit the growth of volatile sulfide-producing bacteria in the oral cavity, and on the other hand, it can also provide zinc ions that react with sulfides.

[0014] An application of the above-mentioned breath-removing composition containing bio-zinc in an oral care product, wherein the oral care product includes any one of toothpaste, tooth powder, mouthwash and mouth freshener.

[0015] Preferably, the weight percentage of biological zinc in the deodorizing composition in the oral care product is 0.1-10%; the weight percentage of organic zinc or inorganic zinc in the deodorizing composition in the oral care product is 0.01-2%. It should be noted that there are many types of organic zinc or inorganic zinc to choose from, and their solubility is different, and the content of them added should be based on complete dissolution.

[0016] A mouthwash comprises, by weight percentage, 0.1%-12% of the above-mentioned breath-removing composition containing biological zinc, 15%-25% of sorbitol, 5%-15% of glycerol, 4%-6% of propylene glycol, 0.5%-1.5% of poloxamer 407, 0.08%-0.12% of flavor, 0.01%-0.03% of saccharin sodium, 0.04%-0.06% of cetylpyridinium chloride, and the balance is water.

[0017] Preferably, the composition comprises, by weight: 0.1%-10% of the above-mentioned deodorizing composition containing bio-zinc, 15%-25% of sorbitol, 5%-15% of glycerol, 4%-6% of propylene glycol, 0.5%-1.5% of poloxamer 407, 0.08%-0.12% of flavor, 0.01%-0.03% of saccharin sodium, 0.04%-0.06% of cetylpyridinium chloride, and the balance is water.

[0018] A method for preparing the above-mentioned mouthwash comprises the following steps:

[0019] (1) Add poloxamer 407 according to weight percentage into deionized water and stir until completely dissolved; add sodium saccharin, cetylpyridinium chloride, and the above-mentioned breath-deodorizing composition and stir until completely dissolved; add sorbitol and glycerol and stir evenly to form an aqueous phase;

[0020] (2) Add the flavor to propylene glycol and mix well to prepare an oil phase;

[0021] (3) Add the oil phase into the water phase under stirring and stir for 20-30 min.

[0022] The technical solution of the present invention has the following advantages:

[0023] For the first time, yeast / zinc fermentation products are used in oral care products with breath-removing functions. While absorbing sulfides in breath, they also solve the problems of low solubility of conventional organic zinc and strong astringency of inorganic zinc ions.

[0024] Yeast / zinc fermentation products are combined with other organic zinc or inorganic zinc to synergistically enhance the absorption efficiency of sulfide in breath and provide a good product taste. The resulting product can suppress sulfide in breath for a long time, freshen breath without obvious astringency or oral mucosal astringency. DETAILED DESCRIPTION

[0025] The technical solution of the present invention is described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] The yeast / zinc fermentation product involved in the present invention, INCI name: Yeast / zinc fermentation product, has been included in the "Catalogue of Used Cosmetic Raw Materials" (2021 edition).

[0027] Unless otherwise specified, other reagents involved in the present invention are common commercially available products.

[0028] Examples 1-5

[0029] The embodiments are designed according to the formula in Table 1.

[0030] Table 1

[0031]

[0032] Comparative Examples 1-6

[0033] The comparative example formula is shown in Table 2.

[0034] Table 2

[0035]

[0036] Preparation process: The preparation process of each embodiment and comparative example in Table 1 and Table 2 adopts the following scheme, and the corresponding addition steps are omitted in the embodiments where no components are added. (1) Add poloxamer 407 to deionized water and stir until completely dissolved; add sodium saccharin, cetylpyridinium chloride, yeast / zinc fermentation product, and zinc citrate and stir until completely dissolved; add sorbitol and glycerol and stir evenly for 5 minutes to prepare an aqueous phase; (2) Add flavor to propylene glycol and stir evenly to prepare an oil phase; (3) Add the oil phase to the aqueous phase under stirring and stir for 20 minutes.

[0037] Performance Testing

[0038] 1. In vitro breath-removal efficacy test - efficacy of absorbing volatile sulfides (hydrogen sulfide) in breath

[0039] 1.1 Test Method

[0040] 9 mL of each mouthwash in the embodiment and the comparative example was loaded into a 22 mL headspace injection bottle, 9 mL of deionized water was used for the blank control group, 1 mL of hydrogen sulfide aqueous solution standard was drawn by a syringe, injected into the headspace injection bottle and quickly sealed, and the hydrogen sulfide content was detected by a gas chromatograph equipped with an FPD+ detector combined with a headspace injector device, the hydrogen sulfide peak area was recorded, and the hydrogen sulfide content in each group of samples was calculated according to the hydrogen sulfide standard curve, and the control group was an aqueous solution.

[0041] 1.2 Test Results

[0042] The hydrogen sulfide absorption rate of each group of samples was calculated according to the following formula: Absorption rate (%) = [(hydrogen sulfide content of blank control group - hydrogen sulfide content of experimental group) / hydrogen sulfide content of blank control group] × 100

[0043] Table 3 In vitro test of the absorption rate of hydrogen sulfide by the samples of each embodiment

[0044] Hydrogen sulfide absorption rate (%) Example 1 96 Example 2 97 Example 3 98 Example 4 97 Example 5 99 Comparative Example 1 3 Comparative Example 2 13 Comparative Example 3 54 Comparative Example 4 66 Comparative Example 5 99 Comparative Example 6 15 Control group 0

[0045] From the results in Table 2, it can be seen that the combination of biological zinc and organic zinc or inorganic zinc in the embodiment has a synergistic effect and a high absorption rate for hydrogen sulfide. Comparative Example 2 only adds zinc citrate, which has limited effect on hydrogen sulfide absorption under limited solubility; yeast / zinc fermentation products are added in Comparative Examples 3-4, which have significant hydrogen sulfide absorption effects; Comparative Example 5, which adds inorganic zinc alone, has a good hydrogen sulfide absorption effect, but it does not bring obvious astringency and astringency at an effective addition amount, limiting its application in oral care products; Comparative Example 6 has a small amount of biological zinc and organic zinc added, and the ratio is 1:1, and the absorption effect is also poor. The reason may be that the activity of zinc ions in the form of organic zinc is not fully released, thereby affecting the synergistic effect of the combination of the two.

[0046] 2. Test on the efficacy of removing sulfide from human breath

[0047] 2.1 Test Method

[0048] The subjects were men and women with oral halitosis who met the inclusion and exclusion criteria. After collecting saliva, the hydrogen sulfide content in the saliva was tested to obtain the baseline value of breath. On the morning of the test, no food was eaten or brushed. 10mL of saliva was collected for gas chromatography to detect the hydrogen sulfide content. The test sample was used to rinse the mouth for 30 seconds and spit out. Then 10mL of saliva was taken within 10 minutes, and the gas chromatograph was used to test the hydrogen sulfide content in the saliva after rinsing the mouth. Each subject used one sample per day, and the test was completed for 5 consecutive days until all 5 samples were tested.

[0049] 2.2 Test Results

[0050] The removal rate of hydrogen sulfide in mouth breath is calculated as follows: Removal rate (%) = [(hydrogen sulfide content before rinsing - hydrogen sulfide content after rinsing) / hydrogen sulfide content before rinsing] × 100

[0051] Table 4 Removal rate of hydrogen sulfide in mouth after gargling

[0052]

[0053] From the results in Table 4, it can be seen that the mouthwash sample of Comparative Example 1 has no obvious effect in removing bad breath, and the hydrogen sulfide removal rate is less than 10%. In Comparative Example 2, only yeast / zinc extract was added as the active ingredient, and the hydrogen sulfide removal rate was also poor. The combined mouthwash of yeast / zinc extract and zinc citrate in Examples 1 and 2 has a significant effect in removing hydrogen sulfide from bad breath, indicating that the two have a synergistic effect.

[0054] 3. Taste test

[0055] 3.1 Test Method

[0056] 20 volunteers were recruited to perform mouthwash tests on the samples of the embodiments and comparative examples, and filled out questionnaires for scoring. The scoring criteria were set as follows: 5 points - no discomfort, no astringency or astringency; 4 points - no obvious discomfort, no obvious astringency or astringency; 3 points - average, with perceptible astringency or astringency; 2 points - obvious discomfort, obvious astringency or astringency; 1 point - very uncomfortable, strong astringency or astringency.

[0057] 3.2 Test Results

[0058] The taste scores of each embodiment were statistically analyzed and the results were averaged. The data are shown in Table 5.

[0059] Table 5 Mouthwash taste test scores of samples in various embodiments

[0060] Taste score Comparative Example 1 4 Comparative Example 2 4.7 Comparative Example 3 4.6 Comparative Example 5 1.1 Example 1 4.9 Example 2 4.9

[0061] From the results in Table 5, it can be seen that the mouthwash samples with added yeast / zinc fermentation products have no astringent or astringent feeling and are easy to use in the mouth.

[0062] Obviously, the above specific implementations are merely examples for clear explanation, and are not intended to limit the implementations. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementations here. The obvious changes or modifications derived therefrom are still within the scope of protection of the present invention.

Claims

1. A breath-removing composition containing biological zinc, characterized in that: The invention comprises biological zinc, which is a yeast / zinc fermentation product, and the content of the biological zinc in the oral care product is 5%-10% by weight; and further comprises organic zinc or inorganic zinc, and the weight ratio of the biological zinc to the organic zinc or inorganic zinc is 100:1-5:1; the organic zinc comprises any one or more of zinc citrate, zinc acetate, and zinc gluconate, and the inorganic zinc comprises any one or more of zinc chloride and zinc sulfate; the weight content of the organic zinc or inorganic zinc in the oral care product is 0.05-2%.

2. The use of a breath-removing composition containing bio-zinc according to claim 1 in oral care products, characterized in that: The oral care product includes any one of toothpaste, tooth powder, mouthwash and mouth freshener.

3. A mouthwash, characterized in that: The composition comprises, by weight percentage: 5.05%-12% of the deodorizing composition containing bio-zinc according to claim 1, 15%-25% of sorbitol, 5%-15% of glycerol, 4%-6% of propylene glycol, 0.5%-1.5% of poloxamer 407, 0.08%-0.12% of flavor, 0.01%-0.03% of saccharin sodium, 0.04%-0.06% of cetylpyridinium chloride, and the balance is water; The breath-removing composition containing biological zinc is composed of biological zinc, organic zinc or biological zinc and inorganic zinc. The biological zinc is a yeast / zinc fermentation product. The content of the biological zinc in the mouthwash is 5%-10% by weight. The weight ratio of the biological zinc to the organic zinc or inorganic zinc is 100:1-5:

1. The organic zinc includes any one or more of zinc citrate, zinc acetate, and zinc gluconate, and the inorganic zinc includes any one or more of zinc chloride and zinc sulfate. The weight content of the organic zinc or inorganic zinc in the mouthwash is 0.05-2%.

4. A mouthwash according to claim 3, characterized in that: The invention comprises, by weight: 5.05%-10% of the breath-removing composition containing bio-zinc according to claim 1, 15%-25% of sorbitol, 5%-15% of glycerol, 4%-6% of propylene glycol, 0.5%-1.5% of poloxamer 407, 0.08%-0.12% of flavor, 0.01%-0.03% of saccharin sodium, 0.04%-0.06% of cetylpyridinium chloride, and the balance is water.

5. A method for preparing a mouthwash according to any one of claims 3 to 4, characterized in that: The following steps are involved: (1) Add poloxamer 407 to deionized water according to weight percentage and stir until completely dissolved; add sodium saccharin, cetylpyridinium chloride, and the deodorizing composition containing biological zinc according to claim 1 and stir until completely dissolved; add sorbitol and glycerol and stir evenly to form an aqueous phase; (2) Add the flavor to propylene glycol and mix well to prepare an oil phase; (3) Add the oil phase into the water phase under stirring and stir for 20-30 min.

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