A culture device and a culture method for butyrate-producing bacteria

Abstract

The present invention discloses a butyric acid bacteria cultivation device and method, which relates to the technical field of butyric acid bacteria cultivation. The device comprises a first-layer cultivation rack; a primary cultivation chamber disposed on the first-layer cultivation rack; a second-layer cultivation rack detachably fixed below the first-layer cultivation rack; a secondary cultivation chamber disposed on the second-layer cultivation rack and interconnected with the primary cultivation chamber; a third-layer cultivation rack detachably fixed below the second-layer cultivation rack; a bacteria collection cylinder mounted on the third-layer cultivation rack; and a slow cultivation assembly disposed in the bacteria collection cylinder and interconnected with the secondary cultivation chamber, the slow cultivation assembly having an opening for butyric acid bacteria liquid to be discharged into the bacteria collection cylinder. This solution arranges several cultivation areas vertically, cultivates butyric acid bacteria in a batch and streamlined manner, and increases the density of the butyric acid bacteria liquid.

Description

Technical Field

[0001] The present invention relates to the technical field of butyric acid bacteria cultivation, and specifically provides a cultivation device and a cultivation method for butyric acid-producing bacteria. Background Art

[0002] Butyric acid bacteria, also known as Butyric acid bacteria, are beneficial bacteria that can inhibit pathogenic bacteria in the intestines and have a strong bowel-regulating effect. Butyric acid bacteria are often cultivated using culture media. Culture media is a technique that artificially grows and reproduces bacteria. Most bacteria can be cultivated artificially by inoculating them onto culture media and allowing them to grow and reproduce. The cultured bacteria are then used for research, identification, and application, making this a highly technical and demanding process.

[0003] In the existing technology, culturing butyric acid bacteria colonies requires culturing a butyric acid bacteria sample first, and then replacing the culture medium to cultivate the butyric acid bacteria after the culture is completed. The amount of culture medium required is large, the culture medium occupies a large area, and the operation of replacing the culture medium multiple times is cumbersome. The anaerobic environment is difficult to control, and batch cultivation of butyric acid bacteria is almost impossible. There is a large market demand for butyric acid bacteria, especially high-density butyric acid bacteria liquid. Summary of the Invention

[0004] The purpose of the present invention is to provide a multi-layered culture rack and a culture chamber installed on the culture rack to realize the vertical arrangement of the culture area, cultivate butyric acid bacteria in batches and in a streamlined manner, and increase the density of the butyric acid bacteria liquid.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] A butyrate-producing bacteria culture device comprises a first layer culture rack;

[0007] A primary culture chamber is provided on the first layer culture shelf;

[0008] a second layer of culture rack detachably fixed below the first layer of culture rack;

[0009] A secondary cultivation chamber provided on the second-layer cultivation shelf and communicated with the primary cultivation chamber;

[0010] a third-layer culture rack detachably fixed below the second-layer culture rack;

[0011] A bacteria collecting cylinder installed on the third-layer culture rack;

[0012] and a slow culture component which is arranged in the bacteria collecting cylinder and communicated with the secondary culture chamber. The slow culture component is provided with an opening for discharging the butyric acid bacteria liquid into the bacteria collecting cylinder.

[0013] As a preferred technical solution of the present application, the primary cultivation chamber and the secondary cultivation chamber are both transparent spheres with cavities opened inside.

[0014] As a preferred technical solution of the present application, both the primary cultivation chamber and the secondary cultivation chamber are provided with a culture fluid replenishment interface and an observation component. The primary cultivation chamber is provided with a strain replenishment interface connected to the butyric acid bacteria liquid tank, wherein the observation component is used to observe the bacterial liquid volume, butyric acid bacteria concentration and butyric acid bacteria growth status in the two cultivation chambers.

[0015] As a preferred technical solution of the present application, columns extending toward each other and used for support are provided between the first-layer culture rack and the second-layer culture rack, as well as between the second-layer culture rack and the third-layer culture rack, and the columns in contact with each other are connected by bolts and nuts.

[0016] As a preferred technical solution of the present application, a support assembly is provided between the first-layer culture rack and the second-layer culture rack, and between the second-layer culture rack and the third-layer culture rack. The support assembly includes a rod body, a sleeve and a pin. The rod body and the sleeve are provided with through holes in the same position. The rod body is inserted into the sleeve, and the pin is inserted into the through hole for locking the sleeve and the rod body.

[0017] As a preferred technical solution of the present application, the slow-incubation component includes a bracket;

[0018] a centrifugal chamber disposed inside the bacteria collecting cylinder, wherein the centrifugal chamber contains a butyric acid bacteria liquid;

[0019] A motor housing rotatably connected to the bottom of the centrifugal chamber and fixedly connected to the bacteria collecting cylinder via the bracket;

[0020] A motor installed in the motor housing and having an output end connected to the centrifugal chamber, the motor being used to drive the centrifugal chamber to rotate;

[0021] A reducer is arranged on the output shaft of the motor.

[0022] As the preferred technical solution of the present application, two primary culture chambers are provided on the first-layer culture rack, and two secondary culture chambers are provided on the second-layer culture rack. The primary culture chambers and the secondary culture chambers are connected one-to-one through straight tubes, and the two secondary culture chambers are connected to the buffer culture assembly through a Y-shaped tube.

[0023] As a preferred technical solution of the present application, the straight tube and the Y-shaped tube are connected in series with a curved tube or a capsule to buffer the speed at which the bacterial liquid enters the secondary culture chamber or the centrifugal chamber.

[0024] As a preferred technical solution of the present application, a weak bacteria blocking member is further connected to one end of the straight tube facing the primary culture chamber, and the weak bacteria blocking member is used to reduce the probability of weak bacteria or dead bacteria in the primary culture chamber entering the secondary culture chamber.

[0025] The weak bacteria barrier is a trumpet-shaped structure with a larger upper end and a smaller lower end.

[0026] The larger end of the weak bacteria blocking member is connected to the straight tube, or the smaller end of the weak bacteria blocking member is connected to the straight tube. Furthermore, the weak bacteria blocking member is made of a permeable membrane that butyric acid bacteria can pass through.

[0027] As a preferred technical solution of the present application, the periphery of the lower half of the primary cultivation chamber and the secondary cultivation chamber spheres are surrounded by trachea, and the trachea is connected to an air pump and a heater through a pipeline.

[0028] According to another aspect of the present invention, a method for culturing butyric acid bacteria is provided, comprising the following steps:

[0029] S1: injecting a butyric acid bacteria liquid and a culture medium into the primary culture chamber and cultivating for a certain period of time to obtain a primary bacterial liquid;

[0030] S2: opening the valve on the connecting pipe between the primary cultivation chamber and the secondary cultivation chamber, injecting the primary bacterial solution into the secondary cultivation chamber and cultivating for a certain period of time to obtain the secondary bacterial solution;

[0031] S3: opening the valve on the connecting pipe between the secondary culture chamber and the buffer culture component, and injecting the secondary bacterial liquid into the buffer culture component;

[0032] S4: The slow culture component overflows high-density bacterial liquid into the bacteria collecting cylinder.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] The multi-layered culture racks and culture chambers installed on the culture racks are arranged to realize the vertical arrangement of the culture area. The operation of changing the culture area is simple and fast, and the culture area occupies a small area. At the same time, the closed anaerobic operation is realized throughout the process, which is highly suitable for the anaerobic cultivation requirements of butyric acid bacteria.

[0035] Real-time observation of the bacterial solution status in each culture area, timely replenishment of the corresponding solution, and adjustment of the operation nodes enable the batch and streamlined cultivation of butyric acid bacteria, making the cultivation process efficient and rapid;

[0036] The butyric acid bacteria liquid is centrifuged so that the strains gather at the edge of the culture area and overflow, thereby obtaining a high-density butyric acid bacteria liquid;

[0037] In the current butyric acid bacteria culture process, high-density culture of bacteria is the development direction. However, in the process of further research, the inventors found that in the culture process, even if the same culture density is ensured in the early stage of culture, the growth of butyric acid bacteria in the middle stage of culture, especially in the late stage of culture, is quite different. The reason is that in the early stage of culture, there are differences in the quality of the bacteria. When there are weak bacteria in the bacteria, the weak bacteria develop and reproduce slowly. In severe cases, there are even too many dead bacteria, which deteriorates the culture environment. Therefore, the culture quality of the bacteria with a high content of weak bacteria in the initial stage is significantly lower than the culture quality of the bacteria with a low content of weak bacteria. Therefore, in the solution of the present application, a weak bacteria blocking member is provided to reduce the probability of weak bacteria or dead bacteria in the primary culture chamber entering the secondary culture chamber, thereby ensuring a good culture effect of butyric acid bacteria.

[0038] When the bacterial liquid in the primary cultivation chamber 2 enters the secondary cultivation chamber, the remaining portion of the bacterial liquid is located between the outer periphery of the weak bacteria blocking member 27 and the primary cultivation chamber 2. Due to the presence of the weak bacteria blocking member, healthy and healthy strong bacteria with good activity can smoothly pass through the weak bacteria blocking member and enter the secondary cultivation chamber. Weak bacteria, due to their low activity, swim more slowly and usually gather at the bottom with dead bacteria. Therefore, these weak and dead bacteria are more likely to be blocked by the weak bacteria blocking member. This reduces the amount of weak and dead bacteria entering the secondary cultivation chamber, reduces the risk of dead bacteria deteriorating the cultivation environment, and thus improves the quality of bacterial culture.

[0039] The weak bacteria blocking member is configured as a structure with adjustable vertical height, and a suitable height can be selected according to actual culture conditions. For example, when the content of bacteria, weak bacteria or dead bacteria in the primary culture chamber is expected to be high, a higher weak bacteria blocking member is used to allow more bacterial liquid to enter the secondary culture chamber after passing through the weak bacteria blocking member. When the quality of the bacteria in the primary culture chamber is estimated to be good, a lower height weak bacteria blocking member is selected to allow the bacterial liquid to enter the secondary culture chamber more quickly, thereby reducing transfer time. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 Schematic diagram of the overall structure of the butyrate-producing bacteria cultivation device in Example 1 of the present invention;

[0041] Figure 2 This is a schematic diagram of the overall structure of the bacteria collecting cylinder in Example 1 of the present invention;

[0042] Figure 3 This is a schematic diagram of the connection of the bacteria collecting cylinder in Example 1 of the present invention.

[0043] Figure 4 Schematic diagram of the process of the butyric acid bacteria cultivation method in Example 1 of the present invention;

[0044] Figure 5This is a schematic diagram of the overall structure of the primary cultivation chamber in Example 2 of the present invention;

[0045] Figure 6 Schematic diagram of the overall structure of the support assembly in the fourth embodiment of the present invention;

[0046] Figure 7 Schematic diagram of the exploded structure of the buffering component in the fifth embodiment of the present invention;

[0047] Figure 8 Schematic diagram of the overall structure of the Y-shaped tube in Example 6 of the present invention;

[0048] Figure 9 This is a schematic diagram of the connection of the trachea in Example 7 of the present invention;

[0049] Figure 10 It is a partial cross-sectional view of one embodiment of the bottom of the primary cultivation chamber of the present invention.

[0050] Markings in the figure: 1. First-layer culture rack; 2. Primary culture chamber; 3. Second-layer culture rack; 4. Secondary culture chamber; 5. Third-layer culture rack; 6. Bacteria collection tube; 7. Slow culture assembly; 8. Opening; 9. Culture fluid replenishment interface; 10. Observation assembly; 11. Bacteria strain replenishment interface; 12. Support assembly; 13. Rod; 14. Sleeve; 15. Pin; 16. Bracket; 17. Centrifugal chamber; 18. Motor cover; 19. Motor; 20. Reducer; 21. Straight pipe; 22. Y-shaped pipe; 23. Bend pipe; 24. Air pipe; 25. Air pump; 26. Heater, 27. Weak bacteria barrier. DETAILED DESCRIPTION

[0051] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0052] Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely represents some embodiments of the present invention. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without creative effort shall fall within the scope of protection of the present invention.

[0053] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features and technical solutions therein may be combined with each other.

[0054] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0055] In the description of the present invention, it should be noted that the terms "upper" and "lower" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or the orientations or positional relationships in which the inventive product is typically placed when in use, or the orientations or positional relationships commonly understood by those skilled in the art. Such terms are intended solely to facilitate the description of the present invention and simplify the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" and the like are used solely for distinction and should not be construed as indicating or implying relative importance.

[0056] Example 1: See Figures 1 to 3 As shown,

[0057] The present embodiment provides a butyric acid bacteria cultivation device, comprising a first-layer cultivation rack 1; a primary cultivation chamber 2 provided on the first-layer cultivation rack 1; the volume of the primary cultivation chamber 2 is greater than that of the secondary cultivation chamber 4, and the reproduction rate of the butyric acid bacteria cultivated in the primary cultivation chamber 2 is restricted after reaching a certain concentration. At this time, the bacterial liquid in the primary cultivation chamber 2 can be discharged to the secondary cultivation chamber 4 to achieve the purpose of expansion; a second-layer cultivation rack 3 detachably fixed below the first-layer cultivation rack 1; a secondary cultivation chamber 4 provided on the second-layer cultivation rack 3 and interconnected with the primary cultivation chamber 2; a third-layer cultivation rack 5 detachably fixed below the second-layer cultivation rack 3; a bacteria collecting cylinder 6 installed on the third-layer cultivation rack 5; and a slow cultivation component 7 provided in the bacteria collecting cylinder 6 and interconnected with the secondary cultivation chamber 4, wherein a portion is provided on the slow cultivation component 7 for discharging the butyric acid bacteria liquid to the bacteria collecting cylinder 6 opening 8; butyric acid bacteria enter the secondary cultivation chamber 4 from the primary cultivation chamber 2 and then enter the slow cultivation component 7 as the cultivation and reproduction stage changes. Relatively independent cultivation areas are set for each stage, which is convenient for adjusting the cultivation environment according to the cultivation requirements of butyric acid bacteria at each stage. In this embodiment, layered cultivation racks are set to save the site area occupied by the cultivation area; save the time required for transferring the strain from one cultivation area to another, and reduce the difficulty of transferring the bacterial solution; achieve full closed anaerobic operation, which is highly suitable for the anaerobic cultivation requirements of butyric acid bacteria; after the bacterial solution in the primary cultivation chamber 2 is transferred, new culture solution can be added thereto, and the cultivation process is streamlined and efficient; the residual butyric acid bacteria caused by incomplete transfer of the cultivation area can be used as strains, saving the amount of strains put in; 0-3 layers of second-layer cultivation racks 3 can be set to adapt to the cultivation of butyric acid bacteria in each cultivation stage, and the scope of application is wide.

[0058] As the preferred technical solution of the present application, the primary cultivation chamber 2 and the secondary cultivation chamber 4 are both transparent spheres with cavities opened inside; the design of the sphere can maximize the ratio of the volume of the two cultivation chambers and the area of ​​the inner wall of the cultivation chamber, reduce the number of bacteria species attached to the inner wall of the cultivation chamber, and reduce the reproduction effect of the boundaries in the bounded culture area on the bacteria species. The transparent material facilitates observation.

[0059] As a preferred technical solution of the present application, the bacteria collecting cylinder 6 is funnel-shaped, and a bacteria liquid discharge outlet is opened at its lower end, and a valve mouth is provided at the bacteria liquid discharge outlet; the bacteria collecting cylinder 6 is transparent for easy observation, and the high-density bacteria liquid discharged from the opening 8 slides downward along the outer wall of the centrifugal chamber 17 and the outer wall of the motor cover 18, and finally gathers in the bacteria collecting cylinder 6. When the valve mouth is opened, the bacteria liquid gathered in the bacteria collecting cylinder 6 can be discharged along the bacteria liquid discharge outlet, thereby obtaining a batch of high-density butyric acid bacteria liquid.

[0060] As a preferred technical solution of the present application, two primary culture chambers 2 are provided on the first-layer culture rack 1, and two secondary culture chambers 4 are provided on the second-layer culture rack 3. The primary culture chambers 2 and the secondary culture chambers 4 are connected one-to-one through straight tubes 21, and the two secondary culture chambers 4 are connected to the buffer culture assembly 7 through a Y-shaped tube 22.

[0061] See also Figure 1-10 As shown, in order to better illustrate the process of the high-density culture method of butyric acid bacteria, this embodiment now proposes a butyric acid bacteria culture method, including the following steps:

[0062] S1: injecting butyric acid bacteria liquid and culture medium into the primary culture chamber 2 and cultivating for a certain period of time to obtain a primary bacterial liquid;

[0063] S2: Opening the valve on the connecting pipe between the primary culture chamber 2 and the secondary culture chamber 4, injecting the primary bacterial solution into the secondary culture chamber 4 and cultivating for a certain period of time to obtain the secondary bacterial solution;

[0064] S3: opening the valve on the connecting pipe between the secondary culture chamber 4 and the slow culture component 7, and injecting the secondary bacterial liquid into the slow culture component 7;

[0065] S4: The slow culture component 7 overflows the high-density bacterial liquid into the bacteria collecting cylinder 6.

[0066] Example 2: See Figure 5 As shown,

[0067] In addition to the above embodiments, this embodiment further provides a culture solution replenishment interface 9 and an observation component 10 on both the primary cultivation chamber 2 and the secondary cultivation chamber 4. The primary cultivation chamber 2 is provided with a strain replenishment interface 11 connected to the butyric acid bacteria liquid tank, wherein the observation component 10 is used to observe the bacterial liquid volume, butyric acid bacteria concentration and butyric acid bacteria growth status in the two cultivation chambers, and the observation component 10 is fixed to the first-layer culture rack 1 or the second-layer culture rack 3 through a connecting rod; the culture solution replenishment interface 9 and the strain replenishment interface 11 are both interconnected with the internal cavity of the cultivation chamber, wherein the culture solution replenishment interface 9 is connected to the culture solution storage tank through a pipeline, and a solenoid valve is installed on the pipeline. The culture solution storage tank in this embodiment is connected to each raw material storage tank through a pipeline, and the corresponding pipelines of each raw material storage tank are also connected in series with a solenoid valve. During operation, each raw material enters the culture solution storage tank and the culture solution enters The incubation process can be driven by gravity or a mixing pump; the observation component 10 consists of an optical microscope, a camera, an image processor and a computing center. The optical microscope is closely attached to the outer surface of the incubation chamber to magnify the butyric acid bacteria liquid in the incubation chamber, and then the camera collects the image of the magnified butyric acid bacteria liquid. The image processor identifies and analyzes the number and characteristics of the butyric acid bacteria in the image and the number and characteristics of the miscellaneous bacteria in the liquid to obtain corresponding data. Using this data as a parameter, a preliminary calculation is performed to obtain the amount of liquid, nutrient solution and the proportion of each component in the nutrient solution that needs to be replenished in the incubation chamber. Then, based on the data obtained from the preliminary calculation and the data of the pipe diameter as parameters, the opening time point and duration of each solenoid valve are calculated again. The control system controls each solenoid valve to open at a specified time point and for a specified duration, thereby achieving the purpose of controlling the proportion of each raw material component in the culture liquid storage tank and replenishing the total amount of culture liquid entering the incubation chamber.

[0068] Example 3:

[0069] On the basis of the above embodiments, this embodiment further provides columns extending toward each other and used for support between the first-layer culture rack 1 and the second-layer culture rack 3, as well as between the second-layer culture rack 3 and the third-layer culture rack 5, and the columns in contact with each other are connected by bolts and nuts; the first-layer culture rack 1 is composed of a plate body 1 on which two primary culture chambers 2 are installed, and a column 1 arranged at the four corners of the lower end of the plate body; the second-layer culture rack 3 is composed of a plate body 2 on which two secondary culture chambers 4 are installed, and a column 2 arranged at the four corners of the plate body 2, and the two ends of the column 2 are respectively located above and below the plate body 2; the third-layer culture rack 5 is composed of a plate body 3 on which a bacteria collecting tube 6 is installed, and a column 3 arranged at the four corners of the plate body 3, and the two ends of the column 3 are respectively located above and below the plate body 3, and the columns are used to support the plate body and provide placement space for the culture chambers and the bacteria collecting tube 6, wherein the columns are connected by bolts and nuts, the connection is firm and stable, and the culture rack is not easy to tip over due to the shift of the center of gravity.

[0070] Example 4: See Figure 6 As shown,

[0071] In addition to the above embodiments, this embodiment further provides support components 12 between the first-layer culture rack 1 and the second-layer culture rack 3, and between the second-layer culture rack 3 and the third-layer culture rack 5. The support component 12 includes a rod body 13, a sleeve 14 and a latch 15. The rod body 13 and the sleeve 14 are provided with through holes in the same position. The rod body 13 is inserted into the sleeve 14, and the latch 15 is inserted into the through hole to lock the sleeve 14 and the rod body 13. The design of the rod body 13, the sleeve 14 and the latch 15 in this embodiment can reduce the difficulty of disassembly and assembly between the culture racks, and the operation of adding or removing the culture racks is simple and fast.

[0072] Example 5: See Figure 7 As shown,

[0073] In addition to the above embodiments, the slow culture component 7 of this embodiment further includes a bracket 16; a centrifugal chamber 17 provided inside the bacteria collecting cylinder 6, wherein the centrifugal chamber 17 contains a butyric acid bacteria liquid; a motor housing 18 rotatably connected to the bottom of the centrifugal chamber 17 and fixedly connected to the bacteria collecting cylinder 6 through the bracket 16; a Y-shaped tube 22 interface is provided at the upper end of the centrifugal chamber 17, and the Y-shaped tube 22 interface is rotatably connected to the Y-shaped tube 22 to avoid constraining the rotational movement of the centrifugal chamber 17; and a motor housing 18 is installed in the motor housing 18 and the output end is connected to the centrifugal chamber 17. The motor 19 is used to drive the centrifugal chamber 17 to rotate; the reducer 20 is arranged on the output shaft of the motor 19; the slow-cultivation component 7 is located inside the bacteria collecting cylinder 6, and the bracket 16 is used to support the motor cover 18, so that the motor cover 18 is suspended relative to the bacteria collecting cylinder 6. The motor 19 rotates the centrifugal chamber 17 at a low speed, which not only allows the butyric acid bacteria contained in the centrifugal chamber 17 to move slowly, improve its temperature balance, and stimulate it to accelerate its reproduction rate, but also gathers the butyric acid bacteria strains to the edge of the centrifugal chamber 17 through centrifugal motion, and as the bacterial liquid in the secondary cultivation chamber 4 The high-density bacterial liquid at the edge of the centrifugal chamber 17 overflows from the opening 8. The lower end of the Y-shaped tube 22 is inserted below the liquid level of the bacterial liquid in the centrifugal chamber 17. The cavity of the centrifugal chamber 17 is bowl-shaped, which prevents the bacterial strains from accumulating at the edge of the lower end of the cavity and being unable to be discharged. In specific operation, the centrifugal chamber 17 is in a rotating state for 20-60 seconds before and after the valve on the Y-shaped tube 22 is opened and closed. The rotation before the valve is opened causes the bacterial strains retained in the centrifugal chamber 17 to move to the edge of the cavity, so that the valve is opened. The bacterial liquid discharged from the opening 8 for the first time after the bacterial liquid is injected is the high-density bacterial liquid. The high-density bacterial liquid can be pumped out from the center of the centrifugal chamber 17 and recycled into the primary culture chamber to save costs. The motor 19 can also be turned off to keep the centrifugal chamber 17 stationary. After the high-density bacterial liquid in the bacteria collecting cylinder 6 is completely discharged, the valve mouth is closed. At this time, the bacterial liquid from the secondary culture chamber 4 is injected into the center of the centrifugal chamber 17. The low-density bacterial liquid at the edge of the centrifugal chamber 17 is squeezed and overflows from the opening 8 and accumulates in the bacteria collecting cylinder 6.

[0074] Example 6: See Figure 8 and 10 As shown,

[0075] This embodiment is further improved upon the above embodiment by providing a curved tube 23 or a capsule connected in series to the straight tube 21 and the Y-shaped tube 22 to buffer the speed at which the bacterial liquid enters the secondary culture chamber 4 or the centrifugal chamber 17. The capsule can also relieve high or negative pressure in each chamber through elastic deformation. In this embodiment, a corrugated curved tube 23 can also achieve the same effect as the capsule.

[0076] Furthermore, a weak bacteria blocking member 27 is connected to one end of the straight tube 21 facing the primary incubation chamber 2. The weak bacteria blocking member 27 is used to reduce the probability of weak bacteria or dead bacteria in the primary incubation chamber 2 entering the secondary incubation chamber 4.

[0077] In the current butyric acid bacteria culture process, high-density culture of bacteria is the development direction, but in the process of further research, the inventors found that in the culture process, even if the same culture density is ensured in the early stage of culture, the growth of butyric acid bacteria in the middle stage, especially in the late stage of culture, also has great differences. The reason is that in the early stage of culture, there are differences in the quality of the bacteria. When there are weak bacteria in the bacteria, the weak bacteria develop and reproduce slowly. In severe cases, there are even too many dead bacteria, which worsens the culture environment. Therefore, the culture quality of the bacteria with a higher content of weak bacteria in the initial stage is significantly lower than the culture quality of the bacteria with a low content of weak bacteria. Therefore, in the scheme of the present application, a weak bacteria blocking member 27 is provided to reduce the probability of weak bacteria or dead bacteria in the primary culture chamber 2 entering the secondary culture chamber 4, thereby ensuring a good culture effect of butyric acid bacteria.

[0078] Furthermore, the weak bacteria blocking member 27 is a trumpet-shaped structure with a larger upper end and a smaller lower end. The larger end of the weak bacteria blocking member 27 is connected to the straight tube 21, or the smaller end of the weak bacteria blocking member 27 is connected to the straight tube 21. In this manner, when the bacterial liquid in the primary incubation chamber 2 enters the secondary incubation chamber 4, the remaining portion of the bacterial liquid is located between the periphery of the weak bacteria blocking member 27 and the primary incubation chamber 2. Due to the presence of the weak bacteria blocking member 27, healthy and healthy strong bacteria with good activity can smoothly pass through the weak bacteria blocking member 27 and enter the secondary incubation chamber 4. Weak bacteria, due to their lower activity, move more slowly and usually gather at the bottom with dead bacteria. Therefore, these weak and dead bacteria are more likely to be blocked by the weak bacteria blocking member 27. This reduces the amount of weak and dead bacteria entering the secondary incubation chamber 4, reduces the risk of dead bacteria deteriorating the culture environment, and thus improves the quality of bacterial culture.

[0079] Furthermore, the weak bacteria blocking member 27 is a mesh plate structure with a plurality of mesh holes or is made of gauze, and the mesh number of the weak bacteria blocking member 27 is 80-150 meshes. The mesh plate or gauze with this mesh number can play a better role in blocking dead bacteria.

[0080] Furthermore, the vertical height of the weak bacteria blocking member 27 is adjustable. By configuring the weak bacteria blocking member 27 as a structure with adjustable vertical height, a suitable height can be selected according to actual culture conditions. For example, if the primary culture chamber 2 is expected to contain a high content of weak bacteria, or dead bacteria, a higher weak bacteria blocking member 27 is used, allowing more bacterial liquid to enter the secondary culture chamber 4 after passing through the weak bacteria blocking member 27. If the quality of the bacteria in the primary culture chamber 2 is estimated to be good, a lower height weak bacteria blocking member 27 is selected, allowing the bacterial liquid to enter the secondary culture chamber 4 more quickly, thereby reducing transfer time. In this embodiment, the weak bacteria blocking member 27 is configured as a structure with adjustable vertical height. The specific height-adjustable structure is a well-known technical means in the art and will not be further elaborated here. For example, a foldable structure can be used.

[0081] Example 7: See Figure 9 As shown,

[0082] This embodiment, based on the above embodiment, further comprises an air tube 24 surrounding the lower half of the primary incubation chamber 2 and the secondary incubation chamber 4. The air tube 24 is connected to an air pump 25 and a heater 26 via a pipe. The bacterial liquid in the primary incubation chamber 2 and the secondary incubation chamber 4 is collected in the lower half of the sphere. The air pump 25 draws in external air and heats the air using the heater 26. The air heated to a certain temperature is then transported to the air tube 24 for circulation. The hot air in the air tube 24 then heats the air near the bacterial liquid, creating a relatively constant temperature environment in the area where the bacterial liquid is located. High-precision temperature control is then used to increase the bacterial liquid growth rate. The above embodiment is intended to illustrate the present invention only and is not intended to limit the technical solutions described herein. Although the present invention has been described in detail with reference to the above embodiments, the present invention is not limited to the specific embodiments described above. Therefore, any modifications or equivalent substitutions of the present invention, as well as all technical solutions and improvements that do not depart from the spirit and scope of the invention, are encompassed by the scope of the claims.

Claims

1. A butyrate-producing bacteria cultivation device, characterized in that: It includes a first-layer culture rack (1); A primary culture chamber (2) provided on the first-layer culture rack (1); a second layer of culture rack (3) detachably fixed below the first layer of culture rack (1); a secondary cultivation chamber (4) disposed on the second-layer cultivation shelf (3) and communicating with the primary cultivation chamber (2); a third-layer culture rack (5) detachably fixed below the second-layer culture rack (3); A bacteria collecting cylinder (6) installed on the third-layer culture rack (5); and a slow culture component (7) disposed in the bacteria collecting cylinder (6) and in communication with the secondary culture chamber (4); the slow culture component (7) is provided with an opening (8) for discharging the butyric acid bacteria liquid into the bacteria collecting cylinder (6); The slow-incubation component (7) includes a bracket (16); a centrifugal chamber (17) disposed inside the bacteria collecting cylinder (6), wherein the centrifugal chamber (17) contains a butyric acid bacteria liquid; A motor housing (18) rotatably connected to the lower portion of the centrifugal chamber (17) and fixedly connected to the bacteria collecting cylinder (6) via the bracket (16); a motor (19) installed in the motor housing (18) and having an output end connected to the centrifugal chamber (17), wherein the motor (19) is used to drive the centrifugal chamber (17) to rotate; a speed reducer (20) disposed on the output shaft of the motor (19); The primary cultivation chamber (2) and the secondary cultivation chamber (4) are connected one-to-one via a straight tube (21), and the two secondary cultivation chambers (4) are connected to the buffer cultivation assembly (7) via a Y-shaped tube (22); The straight tube (21) and the Y-shaped tube (22) are connected in series with a curved tube (23) or a capsule to buffer the speed of the bacterial liquid entering the secondary culture chamber (4) or the centrifugal chamber (17). A weak bacteria blocking member (27) is further connected to one end of the straight tube (21) facing the primary cultivation chamber (2). The weak bacteria blocking member (27) is used to reduce the probability of weak bacteria or dead bacteria in the primary cultivation chamber (2) entering the secondary cultivation chamber (4). The weak bacteria blocking member (27) is a trumpet-shaped structure with a larger upper end and a smaller lower end. The larger end of the weak bacteria blocking member (27) is connected to the straight tube (21), or the smaller end of the weak bacteria blocking member (27) is connected to the straight tube (21), and the weak bacteria blocking member (27) is a mesh plate structure provided with a plurality of mesh holes or is made of gauze, and the mesh number of the weak bacteria blocking member (27) is 80-150 meshes; The slow culture component (7) overflows high-density bacterial liquid into the bacteria collecting cylinder (6).

2. The butyrate-producing bacteria cultivation device according to claim 1, wherein: The primary cultivation chamber (2) and the secondary cultivation chamber (4) are both transparent spheres with cavities formed therein.

3. The butyrate-producing bacteria cultivation device according to claim 2, wherein: The primary cultivation chamber (2) and the secondary cultivation chamber (4) are both provided with a culture solution replenishment interface (9) and an observation component (10). The primary cultivation chamber (2) is provided with a strain replenishment interface (11) connected to a butyric acid bacteria liquid tank, wherein the observation component (10) is used to observe the bacterial liquid volume, butyric acid bacteria concentration and butyric acid bacteria growth status in the two cultivation chambers.

4. The butyrate-producing bacteria cultivation device according to claim 3, wherein: Columns extending toward each other and used for supporting are provided between the first-layer culture rack (1) and the second-layer culture rack (3), as well as between the second-layer culture rack (3) and the third-layer culture rack (5), and the columns in contact with each other are connected by bolts and nuts.

5. The butyrate-producing bacteria cultivation device according to claim 4, wherein: A support assembly (12) is provided between the first-layer culture rack (1) and the second-layer culture rack (3), and between the second-layer culture rack (3) and the third-layer culture rack (5). The support assembly (12) comprises a rod (13), a sleeve (14), and a latch (15). Through holes with the same position are provided on the rod (13) and the sleeve (14). The rod (13) is inserted into the sleeve (14), and the latch (15) is inserted into the through hole for locking the sleeve (14) and the rod (13).

6. The butyrate-producing bacteria cultivation device according to claim 5, wherein: The first-layer culture rack (1) is provided with two primary culture chambers (2), and the second-layer culture rack (3) is provided with two secondary culture chambers (4).

7. The butyrate-producing bacteria cultivation device according to claim 1, wherein: The peripheries of the lower halves of the primary cultivation chamber (2) and the secondary cultivation chamber (4) are surrounded by air tubes (24), and the air tubes (24) are connected to an air pump (25) and a heater (26) through pipelines.

8. A method for culturing butyric acid bacteria, comprising culturing butyric acid bacteria using the butyric acid-producing bacteria culturing device according to any one of claims 1 to 7, comprising the following steps: S1: injecting a butyric acid bacteria liquid and a culture medium into the primary culture chamber (2) and cultivating for a certain period of time to obtain a primary bacterial liquid; S2: opening the valve on the connecting pipe between the primary cultivation chamber (2) and the secondary cultivation chamber (4), injecting the primary bacterial liquid into the secondary cultivation chamber (4) and cultivating for a certain period of time to obtain the secondary bacterial liquid; S3: opening the valve on the connecting pipe between the secondary culture chamber (4) and the slow culture component (7), and injecting the secondary bacterial liquid into the slow culture component (7); S4: the slow culture component (7) overflows the high-density bacterial liquid into the bacterial collection cylinder (6); In the step S2, a first weak bacteria removal step is also included: when the valve on the connecting pipe between the primary cultivation chamber (2) and the secondary cultivation chamber (4) is opened, when the liquid level in the primary cultivation chamber (2) is lower than the upper edge of the weak bacteria blocking member (27), and the height of the liquid level from the upper edge of the weak bacteria blocking member (27) is 1 / 3 of the height of the weak bacteria blocking member (27), the valve is closed, and then new culture fluid is added into the weak bacteria blocking member (27) until it reaches the upper edge of the weak bacteria blocking member (27), and maintained for 2-5 hours, and then the valve is opened again until the liquid level in the primary cultivation chamber (2) is 1 / 15 to 1 / 10 of the height of the weak bacteria blocking member (27), and then the valve is closed.

Images