Detector and method of operation thereof

Abstract

A detector and its operating method are disclosed. The detector includes a detector placement and entry / exit assembly, a detector clamping assembly, and a mixing assembly. The detector placement and entry / exit assembly includes a platform for placing at least one detector; a detector clamping assembly is disposed on a first side of the assembly for clamping the at least one detector; and a mixing assembly is disposed on the first side of the assembly and connected to the detector to mix the sample within the detector. The detector placement and entry / exit assembly is connected to the detector clamping assembly and configured such that the clamping assembly gradually clamps the detector as the platform enters the detector. This detector can cooperate with the detector to achieve fully automated sample detection.

Description

Technical Field

[0001] Embodiments of this disclosure relate to a detector and its operating method. Background Technology

[0002] Microfluidics is a technology for precisely controlling and manipulating microscale fluids. It integrates basic operational units such as sample preparation, reaction, separation, and detection in analytical processes onto a micro- or nanoscale chip, automating the entire analytical process. Microfluidics offers advantages such as low sample consumption, high detection speed, ease of operation, multifunctional integration, small size, and portability, making it a promising technology for applications in biology, chemistry, medicine, and other fields. Summary of the Invention

[0003] At least one embodiment of this disclosure provides a detector, which includes a detector placement and entry / exit assembly, a detector clamping assembly, and a mixing assembly. The detector placement and entry / exit assembly includes a support platform for placing at least one detector; a detector clamping assembly is disposed on a first side of the detector placement and entry / exit assembly for clamping the at least one detector; and a mixing assembly is disposed on the first side of the detector placement and entry / exit assembly for connecting with the detector to mix the sample within the detector. The detector placement and entry / exit assembly is connected to the detector clamping assembly and configured such that when the support platform enters the detector, the detector clamping assembly gradually clamps the detector.

[0004] For example, in the detector provided in at least one embodiment of this disclosure, the detector placement and entry / exit assembly further includes a support and a first tray, the first tray being slidably disposed on the support, and the support platform being disposed on the first tray, configured to allow the support platform to extend or enter the detector by sliding the first tray.

[0005] For example, in the detector provided in at least one embodiment of this disclosure, the detector placement and entry / exit assembly further includes linear bearings disposed on the bracket and located on both sides of the first tray. The detector clamping assembly further includes a pressure plate, a guide shaft, a lower support plate, and a roller assembly. The guide shaft passes through the linear bearings, with one end connected to the pressure plate and the other end connected to the lower support plate. Near the linear bearings, the side of the first tray facing the roller assembly has a guide slope. The roller assembly is disposed on the lower support plate and includes a roller bracket and rollers disposed on the roller bracket. It is configured such that when the support platform enters the detector, the rollers slide along the guide slope to cause the lower support plate to drive the guide shaft to move in the linear bearings, thereby causing the pressure plate to gradually clamp the detector.

[0006] For example, in the detector provided in at least one embodiment of this disclosure, the detection component clamping assembly further includes a spring, which is sleeved on the guide shaft and configured to be compressed during the process of the pressure plate clamping the detection component.

[0007] For example, in the detector provided in at least one embodiment of this disclosure, the detector placement and entry / exit assembly further includes a photoelectric switch and a signal prompting circuit, configured such that after the carrier platform is fully entered into the detector, the light path of the photoelectric switch is blocked, the photoelectric switch is turned on, and the signal prompting circuit sends a prompt that the detector has reached the origin.

[0008] For example, in the detector provided in at least one embodiment of this disclosure, the surface of the pressure plate facing the support stage includes a piercing mechanism configured to pierce the sealing membrane of the liquid storage chamber in the detection component when the pressure plate presses the detection component.

[0009] For example, in the detector provided in at least one embodiment of this disclosure, the detector placement and entry / exit assembly further includes a second tray, and the support platform is elastically connected to the second tray via a stepped shaft and a spring, so that the support platform can move relative to the second tray in a direction perpendicular to the tray surface; the second tray is slidably disposed on the first tray.

[0010] For example, in the detector provided in at least one embodiment of this disclosure, the detector placement and entry / exit assembly further includes a first guide rail, a first baffle, and a second baffle. The first baffle and the second baffle are respectively disposed at opposite ends of the second tray. The second tray is slidably connected to the first tray via the first guide rail and is limited by the first baffle and the second baffle.

[0011] For example, in the detector provided in at least one embodiment of this disclosure, the detector placement and entry / exit assembly further includes a first motor, on which a gear and a rack are fixed, configured to convert the rotational motion of the first motor into linear motion of the first tray on the support.

[0012] For example, in the detector provided in at least one embodiment of this disclosure, the mixing component includes at least one injection pump, each of the at least one injection pump including a cylinder and a plunger rod, the plunger rod moving within the cylinder to achieve evacuation or venting.

[0013] For example, in the detector provided in at least one embodiment of this disclosure, the mixing assembly further includes a first support plate connected to the cylinder, a second support plate connected to the plunger rod, and a second guide rail. The first support plate and the second support plate are slidably disposed on the second guide rail. At least one of the first support plate and the second support plate is configured to move the plunger rod within the cylinder by moving on the second guide rail.

[0014] For example, in the detector provided in at least one embodiment of this disclosure, one end of the cylinder near the placement and entry / exit assembly of the detection component includes a connecting assembly for connecting to the detection component, the connecting assembly including a sealing ring for sealing the cylinder to the detection component.

[0015] For example, at least one embodiment of the detector provided in this disclosure further includes a detection component for detecting samples within the detection component.

[0016] For example, in the detector provided in at least one embodiment of this disclosure, the detection component includes an optical scanning component, the optical scanning component includes at least one optical probe, each of the at least one optical probe includes a light emission path and a light receiving path, for optical detection of the sample in the detection component.

[0017] For example, in the detector provided in at least one embodiment of this disclosure, the optical scanning assembly further includes a conveyor belt, and the at least one optical probe includes a plurality of optical probes connected to the conveyor belt and configured to move with the conveyor belt.

[0018] For example, in the detector provided in at least one embodiment of this disclosure, the light emission paths of the plurality of optical probes are configured to emit light in different wavelength ranges.

[0019] For example, in the detector provided in at least one embodiment of this disclosure, the optical scanning assembly further includes a probe bracket and a third guide rail. The plurality of optical probes are disposed on the probe bracket, a portion of the probe bracket is connected to the conveyor belt, and another portion of the probe bracket is connected to the third guide rail, so that the plurality of optical probes can move along the trajectory of the third guide rail with the conveyor belt.

[0020] For example, the detector provided in at least one embodiment of this disclosure further includes: an air path assembly disposed on a second side of the detector placement and entry / exit assembly, the second side being opposite to the first side, for controlling the opening and closing of the flow channel in the detector by applying air pressure.

[0021] For example, at least one embodiment of the detector provided in this disclosure further includes: a magnet lifting assembly disposed on the second side of the detector placement and entry / exit assembly, the second side being opposite to the first side, and including a magnet, the magnet lifting assembly being configured to control the movement of the magnet.

[0022] For example, in the detector provided in at least one embodiment of this disclosure, the magnet lifting assembly further includes a second motor, a fourth guide rail and a magnet bracket, the magnet is disposed on the magnet bracket, the magnet bracket is slidably disposed on the fourth guide rail, and the second motor is configured to drive the magnet bracket to slide on the fourth guide rail.

[0023] For example, in the detector provided in at least one embodiment of this disclosure, the fourth guide rail is a linear guide rail and is arranged in a direction perpendicular to the surface of the support platform, so that the magnet bracket slides on the fourth guide rail to move closer to or away from the support platform.

[0024] At least one embodiment of this disclosure provides a method for operating a detector, the method comprising: controlling the detector placement and entry / exit assembly to extend the support stage out of the detector to place at least one detector; controlling the detector placement and entry / exit assembly to allow the support stage to enter the detector, the detector pressing assembly gradually pressing the at least one detector; and connecting the mixing assembly to the at least one detector to mix the sample within the detector. Attached Figure Description

[0025] To more clearly illustrate the technical solutions of the embodiments of this disclosure, the accompanying drawings of the embodiments will be briefly described below. Obviously, the drawings described below only relate to some embodiments of this disclosure and are not intended to limit this disclosure.

[0026] Figure 1 This is a schematic diagram of the structure of a detector provided in at least one embodiment of the present disclosure;

[0027] Figure 2 This is another schematic diagram of the detector provided in at least one embodiment of the present disclosure;

[0028] Figure 3 This is a schematic diagram of the structure of the detector placement and entry / exit assembly in the detector provided in at least one embodiment of the present disclosure;

[0029] Figure 4 This is a schematic diagram of the structure of the detection component clamping assembly in the detector provided in at least one embodiment of the present disclosure;

[0030] Figure 5A This is a schematic diagram of the structure of the pressure plate in the pressing assembly of the detection component in the detector provided in at least one embodiment of the present disclosure;

[0031] Figure 5B A schematic diagram of the puncture mechanism of the pressure plate in the pressing assembly of the detection component in the detector provided in at least one embodiment of the present disclosure;

[0032] Figure 5C A cross-sectional schematic diagram showing the cooperation between the puncturing mechanism of the pressure plate in the pressing assembly of the detection component in at least one embodiment of the present disclosure and the detection component;

[0033] Figure 6 This is a schematic diagram of the structure of the mixing component in the detector provided in at least one embodiment of the present disclosure;

[0034] Figure 7 This is a schematic diagram of the structure of the detection component in the detector provided in at least one embodiment of the present disclosure;

[0035] Figure 8 This is a schematic diagram of the structure of the magnet lifting assembly in the detector provided in at least one embodiment of the present disclosure;

[0036] Figure 9 A top view of the substrate of the detection component provided for at least one embodiment of this disclosure; and

[0037] Figure 10 A bottom view of the substrate of a detection component provided in at least one embodiment of this disclosure. Detailed Implementation

[0038] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this disclosure. All other embodiments obtained by those skilled in the art based on the described embodiments of this disclosure without creative effort are within the scope of protection of this disclosure.

[0039] Unless otherwise defined, the technical or scientific terms used in this disclosure shall have the ordinary meaning understood by one of ordinary skill in the art to which this disclosure pertains. The terms “first,” “second,” and similar terms used in this disclosure do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as “comprising” or “including” mean that the element or object preceding the word encompasses the elements or objects listed following the word and their equivalents, without excluding other elements or objects. Terms such as “connected” or “linked” are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as “upper,” “lower,” “left,” and “right” are used only to indicate relative positional relationships, and these relative positional relationships may change accordingly when the absolute position of the described objects changes.

[0040] Typically, detection components used for nucleic acid testing, such as microfluidic chips, include multiple functional chambers such as a sample chamber, reagent chamber, reaction chamber, waste liquid chamber, and detection chamber to realize the entire process of sample addition, mixing, cleaning, and detection. However, due to the complexity and special nature of nucleic acid testing, detection components often cannot achieve fully automated detection on their own.

[0041] At least one embodiment of this disclosure provides a detector, which includes a detector placement and exit assembly, a detector clamping assembly, and a mixing assembly. The detector placement and exit assembly includes a support platform for placing at least one detector; the detector clamping assembly is disposed on a first side of the detector placement and exit assembly for clamping at least one detector; the mixing assembly is disposed on the first side of the detector placement and exit assembly and is connected to the detector to mix the sample within the detector. This detector can be used in conjunction with a detector to achieve fully automated detection of the sample within the detector.

[0042] The detector of this disclosure will be described below through several specific embodiments.

[0043] This disclosure provides at least one embodiment of a detector, Figure 1 and Figure 2 The diagrams show the structure of the detector viewed from different angles. For example... Figure 1 and Figure 2 As shown, the detector includes a detection component placement and entry / exit assembly 1, a detection component pressing assembly 2, and a mixing assembly 4. In some embodiments, the detector may further include a magnet lifting assembly 3, a temperature circulation assembly 5, a detection assembly 6, a pneumatic path assembly 7, and a circuit control assembly 8. For example, the circuit control assembly 8 can be communicatively connected to each of the above-mentioned components to realize the control function of each component, thereby achieving fully automatic detection.

[0044] The structure and function of each component will be described in detail below with reference to the accompanying drawings.

[0045] For example, Figure 3 This is a schematic diagram illustrating the placement and entry / exit of the detection component in a detector provided in at least one embodiment of this disclosure. Figure 3 As shown, the detection component placement and access assembly 1 includes a support stage 1-1 for placing at least one detection component A, or multiple detection components A. Four detection components A are shown in the figure as an example. For example, the detection component A can be a detection chip, such as a microfluidic chip.

[0046] For example, such as Figure 3As shown, the detection component placement and entry / exit assembly 1 also includes a bracket 1-5 (two symmetrical brackets 1-5 shown in the figure) and a first tray 1-3. The first tray 1-3 is slidably disposed on the bracket 1-5, and the support platform 1-1 is disposed on the first tray 1-3. It is configured to allow the support platform 1-1 to extend or enter the detector by sliding the first tray 1-3 on the bracket 1-5, so as to place or remove the detection component A and move the detection component A to a designated position inside the detector.

[0047] For example, in some embodiments, the detection component placement and entry / exit assembly 1 may further include a photoelectric switch 1-9 and a signal indication circuit (not shown), configured such that after the carrier platform 1-1 is fully inserted into the detector, the light path of the photoelectric switch 1-9 is blocked, thereby turning on the photoelectric switch 1-9, and the signal indication circuit sends a prompt indicating that the detection component has reached the origin, thus achieving origin positioning. For example, the photoelectric switch 1-9 includes a photoelectric baffle; after the carrier platform 1-1 enters the detector along with the first tray 1-3, the photoelectric baffle is activated to block the light path of the photoelectric switch 1-9, thereby turning on the photoelectric switch 1-9.

[0048] For example, during use, when it is necessary to place the detection component A into the detector, pull out the support platform 1-1, place the detection component A on the support platform 1-1, and then push the support platform 1-1 into the detector. At this time, the signal prompt circuit sends a prompt that the detection component has reached the origin, and then the detector can be controlled to perform subsequent operations.

[0049] For example, in some embodiments, a detection switch (not shown) may be provided at the bottom of the support platform 1-1 to detect whether a detection component has been placed therein. For example, the detection switch may be a photoelectric switch. When a detection component is placed on the support platform 1-1, the light path of the photoelectric switch is blocked, the photoelectric switch is turned on, indicating that a chip has been placed therein, thereby realizing the self-detection function of determining whether a detection component has been placed therein.

[0050] For example, in some embodiments, the detection component placement and entry / exit assembly 1 may further include a second tray 1-2, with a support platform 1-1 elastically connected to the second tray 1-2 via a stepped shaft and a spring (not shown), so that the support platform 1-1 can move relative to the second tray 1-2 in a direction perpendicular to the surface of the second tray 1-2 (i.e., vertically in the figure). For example, the support platform 1-1 may be elastically connected to the second tray 1-2 via a combination of multiple stepped shafts and springs, for example, the springs are sleeved on the stepped shafts, so that the support platform 1-1 can move vertically relative to the second tray 1-2 under force.

[0051] For example, the second tray 1-2 is slidably disposed on the first tray 1-3. For example, in some examples, the detection component placement and entry / exit assembly 1 further includes a first guide rail 1-13, a first baffle 1-4 and a second baffle 1-10, the first baffle 1-4 and the second baffle 1-10 being disposed at opposite ends of the second tray 1-2, the second tray 1-2 being slidably connected to the first tray 1-3 via the first guide rail 1-13 and being limited by the first baffle 1-4 and the second baffle 1-10.

[0052] For example, the first guide rail 1-13 is a linear guide rail. In this case, the first baffle 1-4 and the second baffle 1-10 are located at both ends of the linear guide rail, so that the second tray 1-2 can move linearly on the first tray 1-3 and be limited by the first baffle 1-4 and the second baffle 1-10.

[0053] For example, the component placement and entry / exit assembly 1 may also include a first motor 1-6, such as a stepper motor. A gear and rack 1-7 are fixed to the output shaft of the first motor 1-6. For instance, through the meshing of the gear and rack, the rotational motion of the first motor 1-6 can be converted into linear motion of the first tray 1-3 on the support 1-5. Thus, the automatic entry and exit of the support platform 1-1 can be achieved through the first motor 1-6.

[0054] For example, a linear guide rail 1-8 is provided on the bracket 1-5, and the first tray 1-3 is slidably disposed on the linear guide rail 1-8, for example, as Figure 3 As shown, linear guide rails 1-8 are respectively provided on the two supports 1-5. The linear guide rails 1-8 can play a role in motion constraint, so that the first tray 1-3 moves on the support 1-5 along the trajectory defined by the linear guide rails 1-8.

[0055] For example, the detection component placement and entry / exit assembly 1 also includes a base plate 1-12 for supporting the various structures of the detection component placement and entry / exit assembly 1.

[0056] Compared to other detection component placement methods, the chip placement and entry / exit component 1 provided in this embodiment of the present disclosure provides a simpler, safer, and more interactive chip placement and entry / exit function, and can also be automated.

[0057] For example, Figure 4 This is a schematic diagram of the structure of the pressing assembly of the detection component in the detector provided in at least one embodiment of the present disclosure. Figure 4 As shown, the detection component clamping assembly 2 is disposed on the first side of the detection component placement and entry / exit assembly 1, i.e. the upper side shown in the figure, for clamping at least one detection component A, so as to facilitate various operations on the detection component A in the future. Figure 4 The situation shown is the state when the detection component clamping assembly 2 clamps the detection component A.

[0058] For example, such as Figure 1 and Figure 2 As shown, the gas path assembly 7 is located on the second side (lower side shown in the figure) where the detection component is placed and enters / exits assembly 1. This second side is opposite to the first side and is used to control the flow path in the detection component by applying air pressure (described in detail later). For example, the gas path assembly 7 includes an air pump 7-1, a gas path plate 7-2, and an air storage tank 7-3. The air pump 7-1 is used to apply air pressure, such as positive or negative pressure. The air storage tank 7-3 stores the gas used by the air pump 7-1 when applying air pressure. The gas path plate 7-2 is used to press against the detection component. For example, when the detection component pressing assembly 2 presses the detection component A, the lower side of the detection component A is pressed against the air circuit board. The air circuit board includes, for example, multiple vent holes or air passages. These multiple vent holes or air passages are connected to the switching valve (the membrane valve part mentioned below, which can be controlled by air pressure) of the flow channel of the detection component to form a closed air passage in the detection component and the air circuit board, so that the air pump can apply air pressure to the detection component A, thereby controlling the opening and closing of the flow channel in the detection component.

[0059] For example, in some embodiments, the detection component placement and entry / exit assembly 1 is connected to the detection component pressing assembly 2, and is configured such that when the support platform 1-1 enters the detector, the detection component pressing assembly 2 gradually presses the detection component.

[0060] For example, such as Figure 3 and Figure 4 As shown, the detection component placement and entry / exit assembly 1 also includes linear bearings 1-11 disposed on the bracket 1-5 and located on both sides of the first tray 1-3, such as... Figure 4 As shown, the detection component clamping assembly 2 also includes a pressure plate 2-1, a guide shaft 2-2, a lower support plate 2-5, and a roller assembly 2-6. The guide shaft 2-2 passes through a linear bearing 1-11. One end of the guide shaft 2-2 (the upper end in the figure) is connected to the pressure plate 2-1, and the other end (the lower end in the figure) is connected to the lower support plate 2-5. Near the linear bearing 1-11, the side of the first tray 1-3 facing the roller assembly 2-6 has a guide slope 1-3-1. The roller assembly 2-6 is mounted on the lower support plate 2-5 and includes a roller bracket and rollers mounted on the roller bracket. It is configured such that when the support platform 1-1 enters the detector (i.e., when moving to the left in the figure), the rollers slide along the guide slope 1-3-1 to cause the lower support plate 2-5 to drive the guide shaft 2-2 to move (move downward) in the linear bearing 1-11, thereby causing the pressure plate 2-1 to gradually clamp the detection component A. In addition, when the bearing platform 1-1 extends the detector (i.e., when it moves to the right in the figure), the roller slides along the guide slope 1-3-1 so that the lower support plate 2-5 drives the guide shaft 2-2 to move (upward) in the linear bearing 1-11, thereby driving the pressure plate 2-1 to gradually move away from the detection component A.

[0061] For example, such as Figure 4 As shown, the detection component clamping assembly 2 may also include a spring 2-3, which is sleeved on the guide shaft 2-2 and configured such that the spring 2-3 is compressed during the process of the pressure plate 2-1 clamping the detection component; in addition, the spring 2-3 can also provide a restoring force during the process of the pressure plate 2-1 moving away from the detection component, thereby assisting the pressure plate 2-1 to gradually move away from the detection component A; furthermore, the spring 2-3 can also provide a supporting function at the initial stage (when the support platform 1-1 is pulled out of the detector) to ensure the initial distance between the air passage plate 7- and the detection component.

[0062] Compared to other clamping methods for detection components, the clamping method provided in this embodiment does not require separate power source control. It only needs to be linked with the detection component placement and entry / exit assembly 1, which is convenient for debugging and saves costs.

[0063] For example, Figure 5A This is a schematic diagram of the structure of the pressure plate in the pressing assembly of the detection component in the detector provided in at least one embodiment of the present disclosure. Figure 5A As shown, in some embodiments, the surface of the pressure plate 2-1 facing the support platform 1-1 (the lower surface in the figure) includes a piercing mechanism 2-7, which is configured to pierce the sealing film of the liquid storage cavity in the detection component A when the pressure plate 2-1 presses the detection component A.

[0064] For example, Figure 5B This is a schematic diagram of the puncture mechanism of the pressure plate in the pressing assembly of the detection component in the detector provided in at least one embodiment of the present disclosure. Figure 5B As shown, the puncture mechanism 2-7 includes a push rod 2-7-1, a needle-shaped protrusion 2-7-2, and a squeezing part 2-7-3. The needle-shaped protrusion 2-7-2 and the squeezing part 2-7-3 protrude from the surface of the push rod 2-7-1, and the protrusion length of the needle-shaped protrusion 2-7-2 exceeds the protrusion length of the squeezing part 2-7-3.

[0065] For example, the needle-shaped protrusion 2-7-2 is used to pierce the sealing membrane of the liquid reservoir of the detection component. The needle-shaped protrusion 2-7-2 is located at the center of the top surface of the push rod 2-7-1, and the size of the needle-shaped protrusion 2-7-2 is smaller than the size of the opening of the liquid reservoir. For example, the needle-shaped protrusion 2-7-2 can be a triangular pyramid with a length of 3mm-5mm, such as 4mm, and a top that is a circle with a diameter of 1mm-2mm, such as 1.5mm.

[0066] For example, there are multiple extrusion parts 2-7-3, which are evenly arranged around the circumference of the needle-shaped protrusion 2-7-2, and the top surface of the extrusion part 2-7-3 is a plane. For example, the extrusion part 2-7-3 can be a boss with a height of 1mm-3mm, such as 2mm, and the number can be, for example, 2-10, and they are arranged circumferentially at equal intervals.

[0067] Figure 5C This is a cross-sectional schematic diagram showing the cooperation between the puncturing mechanism of the pressure plate in the pressing assembly of the detection component in at least one embodiment of the detector provided by this disclosure and the detection component. Figure 5C As shown, during the process of the pressure plate 2-1 pressing the detection component, the piercing mechanism 2-7 acts on the liquid storage chamber A1 of the detection component. The needle-shaped protrusion 2-7-2 is used to pierce the upper sealing film (not shown in the figure) of the liquid storage chamber A1 through the elastic component in the sealing cover A2. For example, as the piercing mechanism 2-7 continues to move downward, the squeezing part 2-7-3 contacts the area around the upper opening of the liquid storage chamber A1, pushing the liquid storage chamber A1 downward. At this time, the multiple squeezing parts 2-7-3 arranged at intervals can prevent them from blocking the opening of the liquid storage chamber A1, so gas can enter the liquid storage chamber A1 through the gaps. For example, the lower end of the liquid storage chamber A1 has another piercing mechanism A3. When the liquid storage chamber A1 moves downward to the piercing mechanism A3, the piercing mechanism A3 can pierce the lower sealing membrane A4 of the liquid storage chamber A1. At the same time, the piercing mechanisms 2-7 can pierce the upper sealing membrane A2 of the liquid storage chamber A1, so that the liquid in the liquid storage chamber A1 can flow out, for example, into the flow channel of the detection component and other functional chambers for subsequent operations. Thus, during the process of the detection component pressing assembly 2 pressing the detection component, the function of piercing the sealing membrane of the liquid storage chamber of the detection component can also be achieved simultaneously.

[0068] For example, Figure 6 This is a schematic diagram of the mixing component in a detector provided in at least one embodiment of the present disclosure. Figure 1 and 6 As shown, the mixing component 4 is disposed on the first side (upper side in the figure) of the detection component placement and entry / exit component 1, and is used to connect with the detection component A to mix the sample in the detection component A.

[0069] For example, such as Figure 6 As shown, the mixing assembly 4 includes at least one injection pump, each of which includes a cylinder 4-6 and a plunger rod 4-9, the plunger rod 4-9 moving within the cylinder 4-6 to achieve evacuation or venting.

[0070] For example, in nucleic acid testing, the mixing component 4 can achieve mixing of the sample and magnetic beads within the detection component, which will be described in detail later.

[0071] For example, such as Figure 6As shown, the mixing assembly 4 also includes a first support plate 4-5 connected to the cylinder 4-6, a second support plate 4-4 connected to the plunger rod 4-9, and a second guide rail 4-3. The first support plate 4-5 and the second support plate 4-4 are slidably disposed on the second guide rail 4-3. At least one of the first support plate 4-5 and the second support plate 4-4 is configured to move on the second guide rail 4-3 to realize the movement of the plunger rod 4-9 within the cylinder 4-6. That is, controlling the movement of one or both of the first support plate 4-5 and the second support plate 4-4 to generate relative movement between the first support plate 4-5 and the second support plate 4-4, thereby allowing the plunger rod 4-9 to move relative to the cylinder 4-6 to realize the extraction or release of air.

[0072] For example, the second guide rail 4-3 is a linear guide rail, so that the first support plate 4-5 and / or the second support plate 4-4 can move linearly on the second guide rail 4-3 to drive the cylinder 4-6 and / or the plunger rod 4-9 to move linearly, so that the plunger rod 4-9 can pump or release air by moving in the cylinder 4-6.

[0073] For example, the mixing assembly 4 may also include motors 4-7 and 4-8 that drive the first support plate 4-5 and the second support plate 4-4 to move respectively. Motors 4-7 and 4-8 are, for example, stepper motors, to drive the first support plate 4-5 and the second support plate 4-4 to move under controlled conditions (e.g., under the control of the circuit control assembly 8).

[0074] For example, the end of cylinder 4-6 near the placement and exit assembly 1 of the detection component includes a connecting assembly 4-10 for connection with the detection component A. The connecting assembly 4-10 includes a sealing ring for sealing the cylinder with the detection component. Thus, by moving the plunger rod 4-9 within cylinder 4-6, for example, evacuation or degassing of the mixing chamber within the detection component A can be achieved, thereby achieving mixing.

[0075] For example, the mixing assembly 4 also includes a bracket 4-1 and a fixing part 4-2 for supporting and fixing the above-mentioned structure of the mixing assembly 4.

[0076] For example, in some embodiments, the detector further includes a detection component 6 for detecting samples within the detection component. Figure 7 This is a schematic diagram of the structure of the detection component in a detector provided in at least one embodiment of this disclosure. For example... Figure 7As shown, the detection component 6 can be an optical scanning component, which includes at least one optical probe 6-1. For example, each optical probe 6-1 includes a light emission path and a light receiving path (not shown), used for optical detection of the sample in the detection component. For example, the light emission path is used to emit light to the sample in the detection component A, and the light receiving path is used to receive the light reflected by the sample. In this case, the detection and analysis of the sample can be achieved, for example, by considering the characteristics of the reflected light or by comparing the difference between the reflected light and the light emitted by the light emission path.

[0077] For example, the optical scanning assembly may also include a conveyor belt 6-4, wherein the at least one optical probe 6-1 comprises a plurality of optical probes 6-1 connected to the conveyor belt 6-4 and configured to move with the conveyor belt 6-1, for example, enabling simultaneous optical scanning of multiple detection components. For example, the optical scanning assembly may also include a motor 6-2 (e.g., a stepper motor) and an idler wheel 6-3, with the conveyor belt 6-4 wound around the idler wheel 6-3, and the motor 6-2 used to drive the conveyor belt 6-4 to move under controlled conditions (e.g., under the control of the circuit control assembly 8).

[0078] For example, the light emission paths of multiple optical probes 6-1 are configured to emit light in different wavelength ranges to suit the detection of different samples or different indicators. For example, each optical probe 6-1 can emit light in different wavelength ranges, such as four, six or more wavelength ranges, to suit the detection of different samples or different indicators.

[0079] Therefore, the optical scanning component provided in the present invention has a multi-channel optical scanning design, strong scalability, and can realize multi-throughput joint inspection.

[0080] For example, in some embodiments, the optical scanning assembly may further include a probe holder 6-7 and a third guide rail 6-5. Multiple optical probes 6-1 are mounted on the probe holder 6-7. Part of the probe holder 6-7 is connected to the conveyor belt 6-4, and another part of the probe holder 6-7 is connected to the third guide rail 6-5, so that the multiple optical probes 6-1 can move along the trajectory of the third guide rail 6-5 with the conveyor belt 6-4. Thus, the third guide rail 6-5 can limit the movement trajectory of the multiple optical probes 6-1.

[0081] For example, the optical scanning assembly also includes a support 6-6 for carrying and supporting the aforementioned structure of the optical scanning assembly.

[0082] For example, Figure 8 This is a schematic diagram of the structure of the magnet lifting assembly in the detector provided in at least one embodiment of this disclosure. Figure 8As shown, the magnet lifting assembly 3 is located on the second side (lower side shown in the figure) of the detection component placement and entry / exit assembly 1, and includes magnet 3-5. The magnet lifting assembly 3 is configured to control the movement of magnet 3-5.

[0083] For example, in nucleic acid testing, the magnet in the magnet lifting assembly 3 can attract the magnetic beads in the detection component when it is close to the detection component, and release the magnetic beads when it is far away from the detection component, thereby assisting in completing functions such as cleaning and mixing the magnetic beads in the detection component.

[0084] For example, the magnet lifting assembly 3 also includes a second motor 3-2 (e.g., a stepper motor), a fourth guide rail 3-3, and a magnet bracket 3-4. A magnet 3-5 is disposed on the magnet bracket 3-4. For example, the magnet 3-5 is elastically connected to the magnet bracket 3-4 by a spring. The magnet bracket 3-4 is slidably disposed on the fourth guide rail 3-3. The second motor 3-2 is configured to drive the magnet bracket 3-4 to slide on the fourth guide rail 3-3. For example, the direction and distance of the magnet bracket 3-4 sliding on the fourth guide rail 3-3 are controllable.

[0085] For example, corresponding to multiple detection components on the support platform 1-1, the magnet lifting assembly 3 may include a combination of a corresponding number of magnet supports 3-4 and magnets 3-5, such as four shown in the figure. In this case, the movement state of the four magnets 3-5 can be controlled to be the same to ensure the consistency of operation on multiple detection components. Of course, in some embodiments, the movement state of the four magnets 3-5 may also be different to achieve different operations on the detection components.

[0086] For example, the fourth guide rail 3-3 is a linear guide rail and is set in a direction perpendicular to the surface of the support platform 1-1, so that the magnet bracket 3-4 slides on the fourth guide rail 3-3 to move closer to or away from the support platform 1-1.

[0087] For example, the magnet lifting assembly 3 also includes a base 3-1 for fixing and supporting the above-described structure of the magnet lifting assembly 3.

[0088] For example, the temperature cycling component 5 includes components such as a heat-conducting part, a cooling part, and a heat dissipation part, and is used at least to realize the temperature cycling of PCR (Polymerase Chain Reaction) to realize the reaction and detection of nucleic acids.

[0089] For example, the circuit control component 8 communicates with the aforementioned components, namely the detection component placement and exit component 1, the detection component pressing component 2, the magnet lifting component 3, the mixing component 4, the temperature circulation component 5, the detection component 6, and the pneumatic circuit component 7, via wired or wireless means, thereby controlling the aforementioned operations of each component. For example, the circuit control component 8 may include any type of controller. For example, the controller may be various types of integrated circuit chips with processing capabilities, possessing various computing architectures, such as Complex Instruction Set Computer (CISC) architecture, Reduced Instruction Set Computer (RISC) architecture, or an architecture implementing multiple instruction set combinations. In some embodiments, the controller 230 may be a microprocessor, such as an x86 processor or an ARM processor, or a digital signal processor (DSP), etc.

[0090] For example, in some embodiments, the circuit control component 8 may further include a memory for storing control instructions for the detector to perform different detections and different processes. For example, the memory unit may be any form of storage medium, such as volatile memory or non-volatile memory, such as semiconductor memory or magnetic media memory, etc., and the embodiments of this disclosure are not limited thereto.

[0091] The detector provided in this embodiment can be implemented as a fully automated microfluidic detector for nucleic acid detection components. During nucleic acid detection, it features nucleic acid extraction, PCR temperature cycling, and fluorescence detection functions, enabling a rapid and convenient measurement process from sample input to result output. This detector offers advantages such as rapid temperature rise and fall, real-time fluorescence detection, and is a fully enclosed detection system. It also has multiple optical detection channels and can simultaneously detect multiple components, offering advantages in cost, space, and time. Furthermore, multiple detectors can be used simultaneously to expand the channels. In applications with high throughput, multiple detectors can be used to form a detection system, thereby achieving modularization of nucleic acid detection.

[0092] At least one embodiment of this disclosure also provides a method for operating a detector, the method comprising: controlling a detection component placement and entry / exit assembly to extend a support stage out of the detector to place at least one detection component; controlling the detection component placement and entry / exit assembly to allow the support stage to enter the detector, a detection component pressing assembly gradually pressing at least one detection component; and connecting a mixing assembly to at least one detection component to mix a sample within the detection component.

[0093] The following description, in conjunction with the accompanying drawings, exemplarily describes a detection component (e.g., a detection chip) used in conjunction with the detector provided in the embodiments of this disclosure, as well as an operation method for using the detector and the detection component together.

[0094] Figure 9A top view of the substrate of a detection chip provided in at least one embodiment of the present disclosure is shown. Figure 10 A bottom view of the substrate of a detection chip provided in at least one embodiment of the present disclosure is shown.

[0095] like Figure 9 and Figure 10 As shown, the detection chip includes a substrate 100, which includes a sample chamber 110, an elution chamber 180, a first cleaning chamber 150, a second cleaning chamber 160, a third cleaning chamber 170, a magnetic bead chamber 120, a first mixing chamber 130, a second mixing chamber 140, a waste liquid chamber 190, and an amplification chamber 200; for example, it also includes a first freeze-drying chamber 102, a second freeze-drying chamber 103, and multiple substrate grooves 100c.

[0096] Multiple fluid channels are formed in the substrate 100. These multiple flow channels include multiple flow paths and multiple membrane valves respectively disposed on some or all of the flow paths. The multiple flow paths include a first flow path 1, a second flow path 2, a third flow path 3, a fourth flow path 4, a fifth flow path 5, a sixth flow path 6, a seventh flow path 7, an eighth flow path 8, a ninth flow path 9, and a tenth flow path 10. Furthermore, each of the first flow path 1, second flow path 2, third flow path 3, fourth flow path 4, fifth flow path 5, sixth flow path 6, seventh flow path 7, eighth flow path 8, ninth flow path 9, and tenth flow path 10 is respectively provided with a first membrane valve V1, a second membrane valve V2, a third membrane valve V3, a fourth membrane valve V4, a fifth membrane valve V5, a sixth membrane valve V6, a seventh membrane valve V7, an eighth membrane valve V8, a ninth membrane valve V9, and a tenth membrane valve V10. These membrane valves are configured to control the connection and disconnection of at least a portion of the corresponding fluid channels, thereby correspondingly closing and opening the flow path in which they are located.

[0097] The following is a detailed explanation of the operation steps for nucleic acid testing using a specific example, in conjunction with the detector and detection components (e.g., microfluidic chips).

[0098] Step S1: Sample addition step.

[0099] The sample to be tested is added to the sample chamber 110 of the detection component. The circuit control component 8 of the detector controls the first motor 1-6 of the detection component placement and entry / exit component 1 to drive the carrier stage 1-1 out of the detector, placing the detection chip onto the carrier stage 1-1. Then, the first motor 1-6 is controlled to drive the carrier stage 1-1 into the detector. At this time, the detection component pressing component gradually presses the detection chip so that the mixing component and other operating components can connect with the detection chip for subsequent operations. When the carrier stage 1-1 is fully inside the detector, the light path of the photoelectric switch 1-9 in the detection component placement and entry / exit component 1 is blocked, the photoelectric switch 1-9 is turned on, and the signal prompt circuit sends a prompt that the detection component has returned to the origin.

[0100] Step S2: Sample and lysing lyophilization release steps.

[0101] For example, the circuit control component 8 of the detector controls the air pump 7-1 of the gas path component 7 to provide air pressure, such as negative pressure, to the first membrane valve V1 to open the first membrane valve V1. At this time, the sample in the sample chamber 110 flows from the sample chamber 110 through the first flow path 1 to the first freeze-drying chamber 102 to dissolve the lysed freeze-dried material stored in the first freeze-drying chamber 102, and then flows to the first mixing chamber 130.

[0102] Step S3: Magnetic bead release step.

[0103] For example, the circuit control component 8 of the detector controls the air pump 7-1 of the gas path component 7 to provide air pressure, such as negative pressure, to the second membrane valve V2 to open the second membrane valve V2. At this time, the magnetic bead solution (i.e., the liquid containing magnetic beads) in the magnetic bead chamber 120 flows to the first mixing chamber 130 through the second flow path 2. Then, the circuit control component 8 controls the air pump of the gas path component 7 to provide air pressure, such as positive pressure, to the second membrane valve V2 to close the second membrane valve V2. At this time, the lysis buffer, the sample, and the magnetic bead solution are mixed in the first mixing chamber 130.

[0104] Step S4: Pyrolysis and mixing step.

[0105] For example, the circuit control component 8 of the detector controls the air pump of the gas path component 7 to provide air pressure, such as negative pressure, to the seventh membrane valve V7 to open the seventh membrane valve V7. Then, the circuit control component 8 controls the connection of the cylinder 4-6 of the mixing component 4 to connect and seal with the first mixing chamber 130, and controls the plunger rod 4-9 to reciprocate within the cylinder 4-6, so that the lysis solution, sample and magnetic bead solution are transported back and forth between the first mixing chamber 130 and the second mixing chamber 140 through the seventh flow path 7 to achieve mixing.

[0106] Then the circuit control component 8 controls the air pump of the air circuit component 7 to provide air pressure, such as positive pressure, to the seventh diaphragm valve section V7 to close the seventh diaphragm valve section V7.

[0107] Step S5: Magnetic bead collection and mixing.

[0108] For example, the circuit control component 8 of the detector controls the magnet lifting component 3 to move the magnet 3-5 to the bottom of the first mixing chamber 130. Then, the circuit control component 8 controls the air pump of the gas path component 7 to provide air pressure, such as negative pressure, to the seventh membrane valve V7 to open the seventh membrane valve V7. Then, the circuit control component 8 controls the plunger rod 4-9 of the mixing component 4 to reciprocate in the cylinder 4-6. At this time, the mixture of sample, lysis buffer and magnetic beads moves back and forth between the first mixing chamber 130 and the second mixing chamber 140 through the seventh flow path 7. Finally, the mixture without magnetic beads stays in the second mixing chamber 140, while the magnetic beads with nucleic acid molecules are attracted by the magnet and stay in the first mixing chamber 130. Then, the circuit control component 8 controls the magnet lifting component 3 to move the magnet 3-5 away from the first mixing chamber 130, and the circuit control component 8 controls the air pump of the gas path component 7 to provide air pressure, such as positive pressure, to the seventh membrane valve V7 to close the seventh membrane valve V7.

[0109] Step S6: Drain the waste liquid.

[0110] For example, the circuit control component 8 of the detector controls the air pump of the air path component 7 to provide air pressure, such as negative pressure, to the tenth membrane valve V10 to open the tenth membrane valve V10. At this time, the mixture without magnetic beads flows as waste liquid from the second mixing chamber 140 through the tenth flow path 10 to the waste liquid chamber 190. Then, the circuit control component 8 controls the air pump of the air path component 7 to provide air pressure, such as positive pressure, to the tenth membrane valve V10 to close the tenth membrane valve V10.

[0111] Step S7: Cleaning fluid release step.

[0112] For example, the circuit control component 8 of the detector controls the air pump of the air path component 7 to provide air pressure, such as negative pressure, to the third membrane valve V3 to open the third membrane valve V3. At this time, the first cleaning liquid in the first cleaning liquid chamber 150 flows from the first cleaning liquid chamber 150 through the third flow path 3 to the first mixing chamber 130 and redissolves the magnetic beads remaining in the first mixing chamber 130. Then, the circuit control component 8 controls the air pump of the air path component 7 to provide air pressure, such as positive pressure, to the third membrane valve V3 to close the third membrane valve V3.

[0113] Step S8: Magnetic bead drying step.

[0114] For example, the circuit control component 8 of the detector controls the heat-conducting part 5-1 of the temperature circulation component 5 to heat the bottom of the first mixing chamber 130 and maintain the temperature at a constant temperature for a certain period of time, for example, within the range of 30-70°C, to dry the surface of the magnetic beads.

[0115] Step S9: Eluent release step.

[0116] For example, the circuit control component 8 of the detector controls the air pump 7-1 of the gas path component 7 to provide air pressure, such as negative pressure, to the sixth membrane valve V6 to open the sixth membrane valve V6. At this time, the eluent in the eluent chamber 180 flows from the eluent chamber 180 through the sixth flow path 6 to the first mixing chamber 130 and redissolves the magnetic beads remaining in the first mixing chamber 130. Then, the circuit control component 8 controls the air pump 7-1 of the gas path component 7 to provide air pressure, such as positive pressure, to the sixth membrane valve V6 to close the sixth membrane valve V6.

[0117] Step S10: Washing and mixing step.

[0118] For example, the circuit control component 8 of the detector controls the air pump 7-1 of the air circuit component 7 to provide air pressure, such as negative pressure, to the seventh membrane valve V7 to open the seventh membrane valve V7. Then, the circuit control component 8 controls the plunger rod 4-9 of the mixing component 4 to reciprocate within the cylinder 4-6, causing the mixture of eluent and magnetic beads to move back and forth between the first mixing chamber 130 and the second mixing chamber 140 through the seventh flow path 7, eventually causing the mixture to remain in the first mixing chamber 130. Then, the circuit control component 8 controls the air pump 7-1 of the air circuit component 7 to provide air pressure, such as positive pressure, to close the seventh membrane valve V7 and control the heat-conducting part of the temperature circulation component 5 to stop heating.

[0119] Step S11: Magnetic bead collection and mixing.

[0120] For example, the circuit control component 8 of the detector controls the magnet lifting component 3 to move the magnet 3-5 to the bottom of the first mixing chamber 130. Then, the circuit control component 8 controls the air pump 7-1 of the gas path component 7 to provide air pressure, such as negative pressure, to the seventh membrane valve V7 to open the seventh membrane valve V7. After that, the circuit control component 8 controls the plunger rod 4-9 of the mixing component 4 to reciprocate in the cylinder 4-6, so that the sample to be amplified moves back and forth between the first mixing chamber 130 and the second mixing chamber 140 through the seventh flow path 7. Finally, the sample to be amplified without magnetic beads stays in the second mixing chamber 140, while the magnetic beads without nucleic acid molecules stay in the first mixing chamber 130 due to the adsorption of the magnet. Then, the circuit control component 8 controls the magnet lifting component 3 to move the magnet 3-5 away from the first mixing chamber 130 and controls the air pump of the gas path component 7 to provide air pressure, such as positive pressure, to the seventh membrane valve V7 to close the seventh membrane valve V7.

[0121] Step S12: Transfer to amplification chamber 200.

[0122] For example, the circuit control component 8 of the detector controls the gas pump 7-1 of the gas path component 7 to provide gas pressure, such as negative pressure, to the eighth membrane valve V8 and the ninth membrane valve V9 to open the eighth membrane valve V8 and the ninth membrane valve V9. At this time, the sample to be amplified first flows through the eighth flow path 8 to the second lyophilization chamber 103 to reconstitute the amplification lyophilized sample in the second lyophilization chamber 103, and then flows to the amplification chamber 200. After filling the amplification chamber 200, it flows through the ninth flow path 9 to the permeate chamber 400. Then, the circuit control component 8 controls the gas pump 7-1 of the gas path component 7 to provide gas pressure, such as positive pressure, to the eighth membrane valve V8 and the ninth membrane valve V9 to close the eighth membrane valve V8 and the ninth membrane valve V9. This transfer step helps to fill the amplification chamber 200 with the sample to be amplified, and excess elution buffer containing nucleic acid molecules will remain in the eighth flow path 8, the ninth flow path 9, or the permeate chamber 400.

[0123] Step S13: Amplification and detection steps.

[0124] For example, the circuit control component 8 of the detector controls the heat conduction, cooling, and heat dissipation sections of the temperature cycling component 5 to perform PCR temperature cycling control on the amplification chamber, so as to amplify the sample to be amplified. Then, the circuit control component 8 controls the detection component 6 to detect the optical properties of the amplified sample to obtain the detection result. For example, the circuit control component 8 controls the movement of the conveyor belt 6-4 of the detection component 6 to drive multiple optical probes 6-1 to perform optical scanning, and by controlling the wavelength range of the light emitted by the light-emitting path of the optical probes 6-1, the detection of a certain index of the sample can be achieved.

[0125] For example, the circuit control component 8 can obtain the detection results of the detection component 6 and save or upload the detection results to the cloud server.

[0126] Therefore, the detection instrument and the detection chip work together to achieve fully automated nucleic acid detection.

[0127] Of course, in other embodiments, the cooperation between the detection component and the detector can also achieve other simplified or more complex operating steps, and the embodiments disclosed herein do not specifically limit this.

[0128] The following points also need to be explained:

[0129] (1) The accompanying drawings of the embodiments of this disclosure only involve the structures involved in the embodiments of this disclosure. Other structures can be referred to the general design.

[0130] (2) For clarity, the thickness of layers or regions in the drawings used to describe embodiments of the present disclosure is enlarged or reduced, i.e., these drawings are not drawn to actual scale.

[0131] (3) Where there is no conflict, the embodiments of this disclosure and the features in the embodiments can be combined with each other to obtain new embodiments.

[0132] The above are merely specific embodiments of this disclosure, but the scope of protection of this disclosure is not limited thereto. The scope of protection of this disclosure shall be determined by the scope of the claims.

Claims

1. A detector, comprising: A component placement and access assembly, including a support platform for placing at least one component; A detection component clamping assembly is disposed on the first side of the detection component placement and entry / exit assembly for clamping the at least one detection component; A mixing component is disposed on the first side of the placement and entry / exit component of the detection component, and is used to connect with the detection component to mix the sample within the detection component; The detection component placement and entry / exit assembly is connected to the detection component pressing assembly, and is configured such that when the carrier platform enters the detector, the detection component pressing assembly gradually presses the detection component; The detection component placement and entry / exit assembly also includes a bracket and a first tray, the first tray being slidably disposed on the bracket, and the support platform being disposed on the first tray, configured to allow the support platform to extend or enter the detector by sliding the first tray; The detection component placement and entry / exit assembly also includes linear bearings mounted on the bracket and located on both sides of the first tray. The detection component clamping assembly further includes a pressure plate, a guide shaft, a lower support plate, and a roller assembly. The guide shaft passes through the linear bearing, and one end of the guide shaft is connected to the pressure plate, while the other end is connected to the lower support plate. Near the linear bearing, the first tray has a guide ramp on the side facing the roller assembly. The roller assembly is disposed on the lower support plate and includes a roller bracket and rollers disposed on the roller bracket. It is configured such that when the support platform enters the detector, the rollers slide along the guide ramp to cause the lower support plate to drive the guide shaft to move in the linear bearing, thereby causing the pressure plate to gradually press the detection component.

2. The detector of claim 1, wherein, The detection component clamping assembly also includes a spring, which is sleeved on the guide shaft and configured to be compressed during the process of the pressure plate clamping the detection component.

3. The detector of claim 1, wherein, The detection component placement and entry / exit assembly also includes a photoelectric switch and a signal indication circuit, configured such that after the carrier platform is fully inserted into the detector, the light path of the photoelectric switch is blocked, the photoelectric switch is turned on, and the signal indication circuit sends a prompt indicating that the detection component has reached the origin.

4. The detector of claim 1, wherein, The surface of the pressure plate facing the support platform includes a piercing mechanism configured to pierce the sealing membrane of the liquid storage chamber within the detection component when the pressure plate presses the detection component.

5. The detector of claim 4, wherein, The puncture mechanism includes a push rod, a needle-shaped protrusion, and a squeezing part. The needle-shaped protrusion and the squeezing part protrude from the surface of the push rod, and the protrusion length of the needle-shaped protrusion exceeds the protrusion length of the squeezing part.

6. The detector according to claim 4, wherein, The detection component placement and entry / exit assembly also includes a second tray, and the support platform is elastically connected to the second tray via a stepped shaft and a spring, so that the support platform can move relative to the second tray in a direction perpendicular to the tray surface of the second tray; The second tray is slidably mounted on the first tray.

7. The detector of claim 6, wherein, The detection component placement and entry / exit assembly further includes a first guide rail, a first baffle, and a second baffle. The first baffle and the second baffle are respectively disposed at opposite ends of the second tray. The second tray is slidably connected to the first tray via the first guide rail and is limited by the first baffle and the second baffle.

8. The detector according to claim 7, wherein, The placement and exit assembly for the detection component also includes a first motor. The output shaft of the first motor is fixed with a gear and a rack, configured to convert the rotational motion of the first motor into the linear motion of the first tray on the support.

9. The detector according to claim 1, wherein, The mixing assembly includes at least one injection pump, each of the at least one injection pump including a cylinder and a plunger rod, the plunger rod moving within the cylinder to achieve evacuation or venting.

10. The detector of claim 9, wherein, The mixing assembly further includes a first support plate connected to the cylinder, a second support plate connected to the plunger rod, and a second guide rail. The first support plate and the second support plate are slidably disposed on the second guide rail, and at least one of the first support plate and the second support plate is configured to move the plunger rod within the cylinder by moving it on the second guide rail.

11. The detector of claim 10, wherein, The end of the cylinder near the placement and exit assembly of the detection component includes a connecting assembly for connecting to the detection component, the connecting assembly including a sealing ring for sealing the cylinder to the detection component.

12. The detector according to claim 1, further comprising a detection component for detecting a sample within the detection component; wherein The detection component includes an optical scanning component, which includes at least one optical probe. Each of the at least one optical probe includes a light emission path and a light receiving path for optical detection of the sample in the detection component. The optical scanning assembly further includes a conveyor belt, and the at least one optical probe includes a plurality of optical probes connected to the conveyor belt and configured to move with the conveyor belt; The light-emitting optical paths of the multiple optical probes are configured to emit light in different wavelength ranges.

13. The detector of claim 12, wherein, The optical scanning assembly also includes a probe bracket and a third guide rail. The plurality of optical probes are mounted on the probe bracket. A portion of the probe bracket is connected to the conveyor belt, and another portion of the probe bracket is connected to the third guide rail, so that the plurality of optical probes can move along the trajectory of the third guide rail with the conveyor belt.

14. The detector according to claim 1, further comprising: An air path assembly is disposed on the second side of the detection component placement and entry / exit assembly, the second side being opposite to the first side, for controlling the opening and closing of the flow channel in the detection component by applying air pressure.

15. The detector according to claim 1, further comprising: A magnet lifting assembly is disposed on the second side of the detection component placement and entry / exit assembly, the second side being opposite to the first side, and includes a magnet. The magnet lifting assembly is configured to control the movement of the magnet.

16. The detector of claim 15, wherein, The magnet lifting assembly also includes a second motor, a fourth guide rail, and a magnet bracket. The magnet is mounted on the magnet bracket, which is slidably mounted on the fourth guide rail. The second motor is configured to drive the magnet bracket to slide on the fourth guide rail.

17. The detector of claim 16, wherein, The fourth guide rail is a linear guide rail and is arranged in a direction perpendicular to the surface of the support platform, so that the magnet bracket can slide on the fourth guide rail to move closer to or further away from the support platform.

18. A method of operating the detector as described in claim 1, comprising: The control assembly for placing and moving the detection component extends the support platform out of the detector to place at least one detection component; The control assembly for placing and moving the detection component causes the support platform to enter the detector, and the detection component pressing assembly gradually presses the at least one detection component; as well as The mixing component is connected to the at least one detection component to mix the sample within the detection component.

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