An ex vivo biological tissue arterial blood supply and venous blood return simulation system

Abstract

The application provides an ex vivo biological tissue arterial blood supply and venous blood return simulation system, a syringe is arranged in the middle of a base, a linear slide bearing and a rotary disc swing arm mechanism are arranged behind the syringe, a slide rod is slidably supported in the linear slide bearing, the slide rod is connected with the rear end of a syringe plunger handle, the slide rod is driven to slide forward and backward by a DC speed reducer through the rotary disc swing arm mechanism, a transfusion bag containing simulated blood is hung on one side of the base through a transfusion rod, two transfusion pipelines are inserted into the transfusion bag, a pipeline connecting assembly is arranged in front of the syringe of the base, and the syringe, the two transfusion pipelines and an external output hose are connected together through the pipeline connecting assembly. When in use, the external output hose is connected to corresponding blood vessels of an ex vivo biological tissue, so that the ex vivo biological tissue can obtain arterial pulsation and blood flow, and continuous venous return can be realized, so that the ex vivo biological tissue can be used to simulate the simulation training operation of vascular anastomosis of a living animal.

Description

Technical Field

[0001] This invention relates to an arterial blood supply and venous blood return simulation system, specifically an ex vivo biological tissue arterial blood supply and venous blood return simulation system for use in microsurgical surgeons to train their operational skills, belonging to the technical field of medical teaching and training equipment. Background Technology

[0002] Microsurgery refers to delicate surgical procedures performed by surgeons using a surgical microscope to magnify small tissues and employing precise microsurgical instruments and suture materials. Currently, most microsurgical procedures require vascular anastomosis, making it one of the most fundamental skills for microsurgeons. This skill demands a high level of proficiency from the surgeon, requiring extensive practical training to master.

[0003] Currently, vascular anastomosis skills training generally uses laboratory animals. Using live animals requires submitting relevant applications and undergoing animal experiment ethics review, a cumbersome process. Furthermore, obtaining live animals is also quite difficult, hindering skills training. Using ex vivo animal tissues for training avoids this cumbersome ethical review process, and ex vivo tissues are easier to obtain and more suitable for skills training. However, the blood vessels in ex vivo animal tissues lack blood flow. Directly using them for vascular anastomosis skills training cannot effectively simulate the arterial and venous blood supply in live animals, making it impossible to assess the actual effectiveness of vascular anastomosis.

[0004] To this end, we have developed a simulated system for arterial blood supply and venous blood return in isolated biological tissues. By connecting it to the corresponding vascular lumen of isolated animal biological tissues, the arteries of isolated biological tissues can obtain arterial pulsation and blood flow, and continuous venous return can also be achieved. In this way, isolated animal biological tissues can be used to simulate vascular anastomosis training operations in live animals. Summary of the Invention

[0005] The purpose of this invention is to provide a simulated system for arterial blood supply and venous blood return of ex vivo biological tissue, which can be used in microsurgical skills training to simulate vascular anastomosis in live animals.

[0006] The specific technical solution adopted in this invention is as follows:

[0007] An ex vivo biological tissue arterial blood supply and venous blood return simulation system includes a base with a horizontally positioned syringe in the center. The syringe's outer sleeve is fixedly supported above the base by a pair of clamp-type supports. Inside the syringe's outer sleeve is a plunger with a rubber stopper at its front end and a handle at its rear end. A mounting plate is located on one side of the base behind the syringe. A horizontal box-type linear slide bearing is mounted on the mounting plate at its front end, supporting a horizontally sliding slide rod. The front end of the slide rod is fixedly connected to the rear end of the syringe plunger handle, and the slide rod drives the syringe plunger to move synchronously. A turntable swing arm mechanism is mounted on the rear end of the mounting plate. The turntable of the turntable swing arm mechanism is driven by an adjustable-speed DC geared motor. The front end of the swing arm is hinged to the rear end of the slide rod, and the rear end is hinged to the eccentric shaft of the turntable. The DC geared motor drives the slide rod to slide back and forth via the turntable swing arm mechanism. A vertically positioned, retractable, and lockable infusion rod is mounted on one side of the base. The upper end of the infusion pole hangs an infusion bag containing simulated blood. The lower end of the infusion bag is connected to infusion tubing #1 and infusion tubing #2, both equipped with drip chambers and flow regulators. A tubing connection assembly is fixed to the base in front of the syringe. This assembly consists of a medical three-way stopcock #1, a medical three-way stopcock #2, a medical one-way valve #1, and a medical one-way valve #2. Port A of the medical three-way stopcock #1 connects to the cone at the front of the syringe jacket, and port B of the medical three-way stopcock #1 connects to the cone at the front of the syringe jacket. The port is connected to the outlet of medical check valve #1, the inlet of medical check valve #1 is connected to the outlet of infusion line #1, port C of medical three-way stopcock #1 is connected to the inlet of medical check valve #2, the outlet of medical check valve #2 is connected to port A of medical three-way stopcock #2, port B of medical three-way stopcock #2 is connected to the outlet of infusion line #2, and port C of medical three-way stopcock #2 is connected to the inlet of external output hose; the diameter of the external output hose is smaller than the diameter of infusion line #2.

[0008] Furthermore, the syringe plunger is fixedly connected to a nut seat at the rear end of the handle, and the front end of the slide rod is connected to the nut seat at the rear end of the syringe plunger handle via a threaded end, and is equipped with a tightening nut for locking.

[0009] Furthermore, the swing arm of the turntable swing arm mechanism is composed of a U-shaped fork head and a hinge bolt. The U-shaped fork head is movably hinged to the rear end of the slide rod by a pin. The rear end of the U-shaped fork head is provided with a screw seat. The hole of the hinge bolt is movably sleeved on the eccentric shaft of the turntable. The bolt head of the hinge bolt is connected to the screw seat at the rear end of the U-shaped fork head and is equipped with a tightening nut for locking.

[0010] Furthermore, the DC geared motor is powered by a steplessly adjustable DC regulated power supply, and the speed of the DC geared motor is changed by adjusting the output voltage of the DC regulated power supply; the voltage regulation range of the DC regulated power supply is 0 to 12V, and the speed regulation range of the DC geared motor is 0 to 120 rpm.

[0011] Furthermore, the connections between the various components of the pipeline connection assembly are all made using matching Luer interfaces, and the connections between the outlets of the two infusion pipelines and the inlet of the external output hose and the pipeline connection assembly are also made using matching Luer interfaces.

[0012] Furthermore, the simulated blood in the infusion bag can be 0.9% sodium chloride injection solution dyed red, so that the operation effect of vascular anastomosis can be observed more intuitively.

[0013] When using the ex vivo biological tissue arterial blood supply and venous blood return simulation system of this invention, first connect a needle to the outlet of the external output tubing and insert it into the corresponding blood vessel lumen of the ex vivo biological tissue, then tie it tightly with sutures. To simulate arterial blood supply, rotate the regulating valves of the #1 and #2 medical three-way stopcocks to the position where all three ports (A, B, and C) are open, and start the DC geared motor to allow arterial pulsation and blood flow in the ex vivo biological tissue's artery. To simulate venous blood return, stop the DC geared motor, rotate the regulating valve of the #2 medical three-way stopcock to the position where ports B and C are open and port A is closed, and completely close the flow regulator of the #1 infusion line to achieve continuous venous return. Therefore, by connecting this invention with ex vivo biological tissue, animal ex vivo biological tissue can be used to simulate vascular anastomosis training operations in live animals, thereby facilitating the training of related skills by microsurgeons. Attached Figure Description

[0014] Figure 1 A three-dimensional schematic diagram of the simulated system for arterial blood supply and venous blood return in isolated biological tissue.

[0015] Figure 2 for Figure 1 A top-down view.

[0016] Figure 3 This diagram illustrates the adjustment of medical three-way valves #1 and #2 during simulated arterial blood supply.

[0017] Figure 4 This diagram illustrates the adjustment of the #1 and #2 medical three-way valves during simulating venous blood return.

[0018] In the diagram: 1-Base, 2-Injector, 2.1-Outer sleeve, 2.2-Core rod, 3-Clamping support, 4-Nut seat, 5-Mounting angle plate, 6-Linear slider bearing, 7-Slide rod, 8-Clamping nut, 9-Turntable, 9.1-Eccentric shaft, 10-DC geared motor, 11-Swing arm, 12-Infusion rod, 13-Infusion bag, 14-Infusion tubing #1, 15-Infusion tubing #2, 16-Drip chamber, 17-Flow regulator, 18-Medical three-way stopcock #1, 19-Medical three-way stopcock #2, 20-Medical one-way valve #1, 21-Medical one-way valve #2, 22-External output hose, 23-U-shaped fork, 24-Hook bolt, 25-DC regulated power supply. Detailed Implementation

[0019] The present invention will be further described below with reference to the accompanying drawings. In the description of the present invention, directional terms such as "front," "rear," "left," "right," "up," and "down" are based on the appendix to the specification. Figure 1 The orientations or positional relationships shown are defined only for the convenience of describing the present invention, and do not refer to a specific orientation that the device or element must have, and should not be regarded as a limitation of the present invention.

[0020] like Figure 1-4As shown, this in vitro biological tissue arterial blood supply and venous blood return simulation system includes a base 1. A horizontally positioned syringe 2 is located in the middle of the base 1. The syringe 2's outer sleeve 2.1 is fixedly clamped and supported above the base 1 by a pair of clamp-type supports 3. Inside the syringe 2's outer sleeve 2.1 is a core rod 2.2. A rubber stopper is located at the front end of the core rod 2.2, and a handle is located at the rear end of the core rod 2.2. A nut seat 4 is fixedly connected to the rear end of the handle. A mounting angle plate 5 is located on one side of the base 1 behind the syringe 2. A horizontal box-type linear slider bearing 6 is mounted on the mounting angle plate 5 at a forward position. The linear slider bearing 6 has a sliding support that allows for horizontal lateral movement. The slide rod 7 has its front end connected to the nut seat 4 at the rear end of the syringe core rod 2.2 via a threaded end, and is locked with a clamping nut 8. The slide rod 7 drives the syringe core rod 2.2 to move synchronously. A turntable swing arm mechanism is mounted on the rear of the mounting plate 5. The turntable 9 of the turntable swing arm mechanism is driven by a DC geared motor 10 with adjustable speed. The front end of the swing arm 11 of the turntable swing arm mechanism is hinged to the rear end of the slide rod 7, and the rear end of the swing arm 11 is hinged to the eccentric shaft 9.1 of the turntable 9. The slide rod 7 is driven to slide back and forth by the DC geared motor 10 through the turntable swing arm mechanism. A vertical support is mounted on one side of the base 1. An infusion pole 12, which is retractable and lockable, is provided. An infusion bag 13 containing simulated blood is suspended from the upper end of the infusion pole 12. The lower end of the infusion bag 13 is connected to infusion tubing 14 (number 1) and infusion tubing 15 (number 2). Both infusion tubing 14 and 15 are equipped with a drip chamber 16 and a flow regulator 17. A tubing connection assembly is fixedly installed on the base 1 in front of the syringe 2. This assembly consists of a medical three-way stopcock 18, a medical three-way stopcock 19, a medical one-way valve 20, and a medical one-way valve 21. Port A of the medical three-way stopcock 18 is connected to the conical tip at the front end of the syringe jacket 2.1. Port B of Medical Three-Way Stopcock 18 is connected to the outlet of Medical One-Way Valve 20, the inlet of Medical One-Way Valve 20 is connected to the outlet of Infusion Line 14, Port C of Medical Three-Way Stopcock 18 is connected to the inlet of Medical One-Way Valve 21, the outlet of Medical One-Way Valve 21 is connected to Port A of Medical Three-Way Stopcock 19, Port B of Medical Three-Way Stopcock 19 is connected to the outlet of Infusion Line 15, and Port C of Medical Three-Way Stopcock 19 is connected to the inlet of External Output Hoses 22; the diameter of External Output Hoses 22 is smaller than the diameter of Infusion Line 15.

[0021] The swing arm 11 of the turntable swing arm mechanism is composed of a U-shaped fork head 23 and a hinge bolt 24. The U-shaped fork head 23 is movably hinged to the rear end of the slide rod 7 by a pin. The rear end of the U-shaped fork head 23 is provided with a screw seat. The hole of the hinge bolt 24 is movably sleeved on the eccentric shaft 9.1 of the turntable 9. The bolt head of the hinge bolt 24 is connected to the screw seat at the rear end of the U-shaped fork head 23 and is equipped with a tightening nut 8 for locking.

[0022] The DC geared motor 10 is powered by a steplessly adjustable DC regulated power supply 25. The speed of the DC geared motor 10 is changed by adjusting the output voltage of the DC regulated power supply 25. The voltage regulation range of the DC regulated power supply 25 is 0 to 12V, and the speed regulation range of the DC geared motor 10 is 0 to 120 rpm.

[0023] All components of the tubing connection assembly are connected using matching Luer connectors. The outlets of the two infusion tubing lines 14 and 15 and the inlet of the external output hose 22 are also connected to the tubing connection assembly using matching Luer connectors. The simulated blood in the infusion bag 23 can be 0.9% sodium chloride injection solution dyed red, allowing for more direct observation of the vascular anastomosis procedure.

[0024] When using this isolated biological tissue arterial blood supply and venous blood return simulation system, first connect the needle to the outlet of the external output hose 22, then insert it into the proximal end of the main artery of the isolated animal biological tissue and tie it tightly with sutures.

[0025] When simulating arterial blood supply, such as Figure 3 As shown, the regulating valves of medical three-way stopcocks 1# (18) and 2# (19) are rotated to the position where all three ports (A, B, and C) are open. The DC geared motor 10 is then started. The DC geared motor 10 drives the slide rod 7 to slide back and forth via the turntable swing arm mechanism. The slide rod 7 drives the syringe plunger 2.2 to perform a lateral reciprocating motion. When the syringe plunger 2.2 is pulled backward, the simulated blood in the infusion bag 13 flows sequentially into the syringe 2 through infusion tubing 1# (14), medical one-way valve 20, and medical three-way stopcock 1# (18). When the syringe plunger 2.2 is pushed forward, the simulated blood in the syringe 2 flows sequentially into syringe 2 through medical three-way stopcock 1# (18), medical one-way valve 21, and medical three-way stopcock 19. The infusion tubing 15 and the external output hose 22 have different diameters. Since the external output hose 22 has a smaller diameter than the infusion tubing 15, most of the simulated blood will overcome gravity and flow back into the infusion bag 13 through the infusion tubing 15. A small portion of the simulated blood will be output through the external output hose 22, forming a pressurized fluid supply. Adjusting the flow regulator 17 on the infusion tubing 15 can change the pressure of the fluid supplied by the external output hose 22. Adjusting the speed of the DC geared motor 10 can change the frequency of the reciprocating motion of the syringe plunger 2.2. This allows for the supply of an intermittent pulsed fluid flow of 0-120 times / minute at a pressure of 0-60 mmHg, enabling the arteries of the isolated biological tissue to experience arterial pulsation and blood flow.

[0026] When simulating venous blood return, the DC geared motor 10 is stopped, such as... Figure 4As shown, rotate the regulating valve of the #2 medical three-way stopcock 19 to the position where ports B and C are open and port A is closed, and completely close the flow regulator 17 of the #1 infusion line 14. The simulated blood in the infusion bag 13 flows sequentially through the #2 infusion line 15 and the #2 medical three-way stopcock 19 into the external output hose 22 and supplies fluid to the outside. Adjusting the flow regulator 17 on the #2 infusion line 15 can change the flow rate of fluid supplied to the external output hose 22. Adjusting the extension height of the infusion rod 12 changes the hanging height of the infusion bag 13, which can change the pressure of fluid supplied to the external output hose 22. Thus, a static liquid flow with a pressure of 0-50 mm water column can be provided to the outside, so that the veins of the isolated biological tissue can obtain continuous reflux.

[0027] When performing simulated venous return alone, the system's output tubing 22 can also be connected to the distal lumen of the vein to be sutured.

[0028] Therefore, by connecting the present invention with the corresponding vascular lumen of isolated animal biological tissue, isolated animal biological tissue can be used to simulate vascular anastomosis training operations in live animals, thereby facilitating the training of microsurgeons in related skills.

[0029] The above illustrations are merely typical embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A simulated system for arterial blood supply and venous blood return in isolated biological tissues, characterized in that: The simulation system is provided with a base (1), and a horizontally arranged syringe (2) is provided in the middle of the base (1). The outer sleeve (2.1) of the syringe (2) is fixedly clamped and supported above the base (1) by a pair of clamp-type supports (3). The syringe (2) has a core rod (2.2) inside the outer sleeve (2.1). The front end of the core rod (2.2) is provided with a rubber stopper, and the rear end of the core rod (2.2) is provided with a handle. The base (1) has a mounting angle plate (5) on one side behind the syringe (2). The mounting angle plate (5) is equipped with a horizontal box-type linear slider bearing (6) at the front position. The linear slider bearing (6) is slidably supported by a slide rod (7) that can slide horizontally. The front end of the slide rod (7) The core rod (2.2) of the syringe (2) is fixedly connected to the rear end of the hand, and the core rod (2.2) of the syringe (2) is driven synchronously by the slide rod (7); the mounting plate (5) is equipped with a turntable swing arm mechanism at the rear position. The turntable (9) of the turntable swing arm mechanism is driven by a DC geared motor (10) with adjustable speed. The front end of the swing arm (11) of the turntable swing arm mechanism is hinged to the rear end of the slide rod (7), and the rear end of the swing arm (11) is hinged to the eccentric shaft (9.1) of the turntable (9). The slide rod (7) is driven to slide back and forth by the DC geared motor (10) through the turntable swing arm mechanism; a vertically set and retractable and lockable infusion rod (12) is mounted on one side of the base (1). The upper end of the infusion rod (12) is hung with an infusion bag (13) containing simulated blood. The lower end of the infusion bag (13) is connected to an infusion tubing (14) and an infusion tubing (15). Both infusion tubing are equipped with a drip chamber (16) and a flow regulator (17). The base (1) is fixedly equipped with a tubing connection assembly in front of the syringe (2). The tubing connection assembly consists of a medical three-way stopcock (18), a medical three-way stopcock (19), a medical one-way valve (20), and a medical one-way valve (21). The A port of the medical three-way stopcock (18) is connected to the cone at the front end of the syringe jacket (2.1). Port B of 18) is connected to the outlet of medical check valve 1 (20), the inlet of medical check valve 1 (20) is connected to the outlet of infusion line 1 (14), port C of medical three-way stopcock 1 (18) is connected to the inlet of medical check valve 2 (21), the outlet of medical check valve 2 (21) is connected to port A of medical three-way stopcock 2 (19), port B of medical three-way stopcock 2 (19) is connected to the outlet of infusion line 2 (15), and port C of medical three-way stopcock 2 (19) is connected to the inlet of external output hose (22); the diameter of external output hose (22) is smaller than the diameter of infusion line 2 (15).

2. The isolated biological tissue arterial blood supply and venous blood return simulation system according to claim 1, characterized in that: The syringe (2) has a nut seat (4) fixedly connected to the rear end of the handle of the core rod (2). The front end of the slide rod (7) is connected to the nut seat (4) at the rear end of the handle of the syringe (2) through a threaded end, and is equipped with a tightening nut (8) for locking.

3. The isolated biological tissue arterial blood supply and venous blood return simulation system according to claim 1, characterized in that: The swing arm (11) of the turntable swing arm mechanism is connected by a U-shaped fork (23) and a hinge bolt (24). The U-shaped fork (23) is movably hinged to the rear end of the slide rod (7) by a pin. The rear end of the U-shaped fork (23) is provided with a screw seat. The hole of the hinge bolt (24) is movably sleeved on the eccentric shaft (9.1) of the turntable (9). The bolt head of the hinge bolt (24) is connected to the screw seat at the rear end of the U-shaped fork (23) and is equipped with a tightening nut (8) for locking.

4. The isolated biological tissue arterial blood supply and venous blood return simulation system according to claim 1, characterized in that: The DC geared motor (10) is powered by a stepless adjustable DC regulated power supply (25), and the speed of the DC geared motor (10) is changed by adjusting the output voltage of the DC regulated power supply (25).

5. The isolated biological tissue arterial blood supply and venous blood return simulation system according to claim 4, characterized in that: The voltage regulation range of the DC regulated power supply (25) is 0 to 12V, and the speed regulation range of the DC geared motor (10) is 0 to 120 rpm.

6. The isolated biological tissue arterial blood supply and venous blood return simulation system according to claim 1, characterized in that: All components of the pipeline connection assembly are connected using matching Luer interfaces. The connection between the outlet of the two infusion pipelines and the inlet of the external output hose (22) and the pipeline connection assembly is also connected using matching Luer interfaces.

7. The isolated biological tissue arterial blood supply and venous blood return simulation system according to claim 1, characterized in that: The simulated blood in the infusion bag (13) is made of 0.9% sodium chloride injection solution dyed red.

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