Simulated solid-state fermentation device and simulated solid-state fermentation method

Abstract

The present invention discloses a simulated solid-state fermentation device and a simulated solid-state fermentation method. The simulated solid-state fermentation device includes a fermentation tank, a shelf, and a circulation structure. The internal space of the fermentation tank includes a reaction part and a receiving part. The reaction part is arranged above the receiving part; a shelf is arranged in the reaction part, a groove is arranged on the plate surface of the shelf, and an absorption structure is arranged above the shelf; the circulation structure includes a first pipeline and a second pipeline. The receiving part is connected to the first end of the shelf through the first pipeline, and the receiving part is connected to the second end of the shelf through the second pipeline. In the present invention, the culture medium is in the receiving part, the culture medium is introduced into the groove of the shelf along the first pipeline, the absorption structure absorbs the culture medium from the groove, the culture medium that is not completely absorbed enters the second pipeline through the groove and flows back to the receiving part, forming a circulation absorption of the absorption structure. When the fermentation reaction starts, the strain is introduced into the groove along the first pipeline, and is circulated and absorbed by the absorption structure, so that the bacteria body undergoes a fermentation reaction, and the reaction parameters are easily monitored.

Description

Technical Field

[0001] The invention relates to the field of fermentation reaction, and in particular to a simulated solid-state fermentation device and a simulated solid-state fermentation method. Background Art

[0002] Solid-state fermentation refers to a biological reaction process using one or more microorganisms in a water-soluble solid matrix with a certain humidity in the absence of or almost no free water. Considering the nature of the biological reaction process, solid-state fermentation is a biological reaction process with the gas phase as the continuous phase. Solid-state fermentation is limited to microorganisms that grow under low humidity conditions, and the possible processes and products are relatively limited. Various parameters in the solid state are not easy to detect, especially various probes for liquid fermentation are not suitable for solid fermentation. pH value, humidity, and matrix concentration are not easy to control, making it difficult to ensure consistent fermentation conditions for each batch, and product quality is unstable. At the same time, the culture time of solid fermentation is long, and the yield and production capacity are low. Summary of the invention

[0003] In order to solve at least one of the above technical problems, the present invention provides a simulated solid-state fermentation device and a simulated solid-state fermentation method, and the technical solutions adopted are as follows:

[0004] The present invention provides a simulated solid-state fermentation device, which comprises a fermentation tank, a shelf, and a circulation structure. The internal space of the fermentation tank comprises a reaction part and a receiving part. The reaction part is connected with the receiving part, and the reaction part is arranged above the receiving part. The shelf is arranged in the reaction part, and the plate surface of the shelf is provided with a groove, and a culture medium or a strain can flow along the groove. An absorption structure is arranged above the shelf, and the absorption structure is used to absorb the culture medium or the strain inside. The circulation structure comprises a first pipeline and a second pipeline. The receiving part is connected to a first end of the shelf through the first pipeline, and the first pipeline is connected to the groove. The receiving part is connected to a second end of the shelf through the second pipeline, and the second pipeline is connected to the groove.

[0005] The embodiments of the present invention have at least the following beneficial effects: In the present invention, the culture medium is in the accommodating portion, the culture medium is introduced into the groove of the shelf along the first pipeline, the absorption structure adsorbs the culture medium from the groove, the culture medium that is not completely adsorbed enters the second pipeline through the groove, and flows back to the accommodating portion along the second pipeline, forming a cyclic absorption of the absorption structure, the culture medium fully enters the absorption structure, so that the absorption structure simulates solid raw materials, when the fermentation reaction starts, the bacteria are introduced into the groove along the first pipeline, and cyclically absorbed by the absorption structure, so that the bacteria are in the environment of the culture medium to perform a simulated solid-state fermentation reaction, which makes it easy to monitor reaction parameters and control reaction conditions.

[0006] In certain embodiments of the present invention, a plurality of shelves are provided, each of the shelves is longitudinally distributed in the reaction section, there is a height difference between adjacent shelves, and adjacent shelves are connected by connecting rods, and each of the shelves can slide on the connecting rods to change the height of the shelves.

[0007] In some embodiments of the present invention, a plurality of the grooves are provided on the shelf, the grooves are arranged in parallel, and the grooves are connected in sequence from end to end.

[0008] In some embodiments of the present invention, a first liquid collecting component is provided on the first pipeline, the first liquid collecting component is hollow and connected to the groove, and a second liquid collecting component is provided on the second pipeline, the second liquid collecting component is hollow and connected to the groove.

[0009] In certain embodiments of the present invention, the first liquid collection component is connected to a first soft pipe, the first soft pipe is connected to the groove, and the number of the first soft pipes is the same as the number of the shelves, and the second liquid collection component is connected to a second soft pipe, the second soft pipe is connected to the groove, and the number of the second soft pipes is the same as the number of the shelves.

[0010] In certain embodiments of the present invention, the simulated solid-state fermentation device also includes a hydraulic device, which is connected to the shelves, and the hydraulic device can push the shelves to reduce the distance between the shelves. The accommodating portion is provided with a supporting structure, and the supporting structure is arranged below the shelves, and the supporting structure is used to support the shelves.

[0011] In some embodiments of the present invention, a liquid storage tank is disposed in the accommodating portion, the liquid storage tank is communicated with the first pipeline, and the liquid storage tank is communicated with the second pipeline.

[0012] In certain embodiments of the present invention, an air intake pipeline is provided on the fermentation tank, a gas filter is provided on the air intake pipeline, and the air intake pipeline is connected to the first liquid collecting component.

[0013] In certain embodiments of the present invention, a simulated solid-state fermentation device further includes a monitoring device, which includes a pressure gauge, a thermometer, and a fermentation monitoring device. The pressure gauge is arranged on the fermentation tank, and the thermometer and the fermentation monitoring device are arranged on the first pipeline.

[0014] The present invention provides a simulated solid-state fermentation method, comprising:

[0015] Adding culture medium into the accommodating part, squeezing the absorption structure, introducing steam into the first pipeline, the steam enters the second pipeline through the shelf, and the steam diffuses from the second pipeline to various positions inside the fermentation tank;

[0016] The first pipeline continuously introduces the culture medium into the groove, releases the absorption structure, and the absorption structure absorbs the culture medium. The unabsorbed culture medium flows back to the containing part along the second pipeline, so that the absorption structure circulates and absorbs the culture medium.

[0017] Lowering the temperature inside the fermentation tank, connecting the bacteria from the first pipeline, introducing the bacteria into the groove, the absorption structure absorbs the bacteria, and the unabsorbed bacteria flow back to the accommodation part along the second pipeline, and further circulate the bacteria into the groove, and the absorption structure circulates and absorbs the bacteria;

[0018] After the fermentation product accumulates, the absorption structure is squeezed and the fermentation product flows to the receiving part, thereby being discharged from the fermentation tank.

[0019] The embodiments of the present invention have at least the following beneficial effects: In the present invention, steam is introduced into the first pipeline, and the steam is distributed in the fermentation tank through the groove on the shelf and the second pipeline, and the fermentation tank is sterilized by wet heat, which can easily denature or coagulate the protein, and finally lead to the death of the microorganisms, and the sterilization efficiency is high. After sterilization, the absorption structure circulates and absorbs the culture medium through the first pipeline and the second pipeline, and the absorption structure circulates and absorbs the bacteria, so that the bacteria ferment in the culture medium, and the reaction products are generated in the absorption structure. The absorption structure simulates solid-state fermentation, the bacteria and the culture medium are evenly mixed, and the reaction environment is easy to control, thereby adjusting the efficiency of the fermentation reaction, and it is easy to operate.

[0020] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0022] Figure 1 It is a structural schematic diagram of a simulated solid-state fermentation device of the present invention;

[0023] Figure 2 It is a side view of a natural state of a shelf in a simulated solid-state fermentation device of the present invention;

[0024] Figure 3 It is a side view of a shelf extrusion state in a simulated solid-state fermentation device of the present invention;

[0025] Figure 4 It is a top view of a shelf in a simulated solid-state fermentation device of the present invention;

[0026] Figure 5 It is a steam flow diagram of a sterilization process of a simulated solid-state fermentation device of the present invention;

[0027] Figure 6It is a flow diagram of the culture medium in the process of culture medium absorption in a simulated solid-state fermentation device of the present invention;

[0028] Figure 7 It is a gas flow diagram during a reaction process of a simulated solid-state fermentation device of the present invention;

[0029] Figure 8 It is an extrusion direction diagram of a shelf extrusion state of a simulated solid-state fermentation device of the present invention.

[0030] Reference numerals:

[0031] 101. fermentation tank; 102. reaction part; 103. containing part;

[0032] 201. shelf; 202. groove; 203. absorption structure; 204. connecting rod;

[0033] 301. Hydraulic device; 302. Support structure;

[0034] 401. First pipeline; 402. Second pipeline; 403. First liquid collecting component; 404. Second liquid collecting component; 405. First soft pipeline; 406. Second soft pipeline;

[0035] 501. Liquid storage tank; 502. Air intake pipe. DETAILED DESCRIPTION

[0036] This section will combine Figures 1 to 8 Embodiments of the present invention are described in detail, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0037] In the description of the present invention, it should be understood that if the terms "center", "middle", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "axial", "radial", "circumferential" and the like appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. The features defined as "first" and "second" are used to distinguish the feature names, rather than having special meanings. In addition, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0038] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0039] The embodiment of the present invention provides a simulated solid-state fermentation device, including a fermentation tank 101, a shelf 201, and a circulation structure. This device is a microbial rapid fermentation device that combines the advantages of solid-state fermentation and avoids the disadvantages of solid-state fermentation. It is convenient for an inert material with the ability to absorb liquid to simulate a solid raw material after absorbing the culture medium. After the fermentation is completed, the fermentation liquid can be obtained by squeezing, and there is no need to deal with the waste residue remaining in the solid raw material after the solid-state fermentation is completed, which reduces the discharge of waste residue, is green and environmentally friendly, and saves time. At the same time, the comprehensive solid-state fermentation can produce special products, or produce special flavors, and improve the nutritional value.

[0040] like Figure 1 As shown, the fermentation tank 101 is relatively closed to prevent the external environment from causing adverse effects on the fermentation reaction. The internal space of the fermentation tank 101 includes a reaction part 102 and a receiving part 103. During the culture medium setting process, the receiving part 103 is used to temporarily store the culture medium; during the strain access process, the receiving part 103 is used to temporarily store the strain; in addition, during the extraction of the reaction product, the receiving part 103 is used to store the reaction product. The strain and the culture medium are mixed in the reaction part 102, and the mixed strains produce a fermentation reaction in the reaction part 102, thereby generating a reaction product. It can be understood that the reaction part 102 is connected to the receiving part 103, which is convenient for passing the strain and the culture medium into the reaction part 102. Specifically, the reaction section 102 is arranged above the accommodating section 103, and the raw materials enter and leave the reaction section 102 through different channels. When the raw materials are introduced from the low-position accommodating section 103 into the high-position reaction section 102, additional power is required to overcome the gravity of the raw materials. When the raw materials are introduced from the high-position reaction section 102 into the low-position accommodating section 103, the raw materials can flow down naturally due to their gravity.

[0041] The shelf 201 is arranged in the reaction part 102. The shelf 201 is used to store raw materials and make the raw materials flow on the shelf 201. Furthermore, the shelf 201 is provided with a groove 202. The culture medium or strain can flow under the guidance of the groove 202 to prevent the raw materials from flowing freely on the shelf 201 in an uncontrollable manner. An absorption structure 203 is arranged above the shelf 201. The absorption structure 203 is located on the groove 202. The absorption structure 203 has many tiny holes. In the natural state, the absorption structure 203 can absorb the liquid contacted by the outside through the holes; in the compressed state, the internal space generated by the holes is compacted, and the ability of the absorption structure 203 to absorb external liquid decreases. Specifically, the absorption structure 203 is made of sponge. When the absorption structure 203 is in a natural state, the absorption structure 203 contacts the culture medium or bacterial strain in the groove 202, and the culture medium or bacterial strain enters the absorption structure 203; when the absorption structure 203 is in a compressed state, the absorption structure 203 can absorb a small amount of culture medium or bacterial strain, while a large amount of culture medium or bacterial strain continues to flow along the groove 202 to the subsequent structure.

[0042] like Figures 2 to 3 As shown, in some examples, a plurality of shelves 201 are provided, and the plurality of shelves 201 are longitudinally distributed in the reaction section 102. It can be understood that each shelf 201 is at a different height position, and there is a certain height difference between adjacent shelves 201. Since an absorption structure 203 is provided on each shelf 201, the absorption structure 203 exists in the space between the shelves 201.

[0043] Furthermore, adjacent shelves 201 are connected by connecting rods 204, which are arranged on both sides of the shelves 201. Specifically, both sides of the shelves 201 are provided with extensions, and the extensions are provided with connection holes. The connecting rods 204 pass through the connection holes on the corresponding extensions of the two adjacent shelves 201, so that the two adjacent shelves 201 are movably connected, that is, the shelves 201 can slide on the connecting rods, thereby changing the distance between the adjacent shelves 201. It can be understood that a protruding structure is provided on the connecting rod 204, and the outer diameter of the protruding structure is larger than the hole diameter of the connection hole, so as to prevent the shelf 201 from falling off the connecting rod 204. At the same time, in order to avoid the interference of the multiple connecting rods 204 connected to the shelf 201, the positions of the multiple connecting holes on the extension part have a certain distance. It can be understood that the shelf 201 located in the middle needs to be connected to the upper adjacent shelf 201 through the connecting rod 204, and also needs to be connected to the lower adjacent shelf 201 through the connecting rod 204. Therefore, two connecting holes are set on the extension part of the shelf 201 located in the middle part. The shelf 201 located at the bottom only needs to be connected to the upper adjacent shelf 201 through the connecting rod 204, so one connecting hole is set on the extension part of the shelf 201 located at the bottom.

[0044] Adjacent shelves 201 extrude the absorption structure 203 by changing the distance between the shelves 201. Since there is no shelf 201 above the top shelf 201, a pressure plate is set above the top shelf 201. The pressure plate and the top shelf 201 are also connected by a connecting rod 204. By changing the distance between the pressure plate and the top shelf 201, the absorption structure 203 set on the top shelf 201 can be squeezed.

[0045] like Figure 4 As shown, in some examples, a plurality of grooves 202 are provided on the shelf 201, and the plurality of grooves 202 are arranged in parallel, and the grooves 202 are connected to each other, and the groove 202 located at the first end of the shelf 201 is connected to the edge of the first end of the shelf 201, and the groove 202 located at the second end of the shelf 201 is connected to the edge of the second end of the shelf 201, so as to facilitate the introduction of the bacterial species or culture medium in the accommodating portion 103 from the first end of the shelf 201 to the shelf 201, and also facilitate the introduction of the bacterial species or culture medium in the groove 202 from the second end of the shelf 201. Specifically, the plurality of grooves 202 are connected end to end in sequence, and the travel path of the culture medium or bacterial species on the shelf 201 is increased, so as to facilitate the absorption structure 203 to fully absorb the culture medium or bacterial species.

[0046] In some examples, the simulated solid-state fermentation device further includes a hydraulic device 301, which extends into the interior of the reaction tank and is connected to the pressing plate. When the hydraulic structure pushes the pressing plate downward, the distance between the pressing plate and the shelf 201 and between adjacent shelves 201 are reduced, thereby squeezing the absorption structure 203 on the shelf 201. Further, a support structure 302 is provided inside the accommodating portion 103. When the absorption structure 203 is in a natural state, the distance between the shelves 201 is large, and there is a certain distance between the shelf 201 at the bottom and the support structure 302; when the absorption structure 203 is in a compressed state, the distance between the shelves 201 is small, and the shelf 201 at the bottom abuts the support structure 302 during the downward movement. When the bottom shelf 201 abuts the support structure 302, the height of the bottom shelf 201 no longer changes, and the remaining shelves 201 move further downward, thereby fully squeezing the absorption structure 203. When each absorption structure 203 needs to be released, the hydraulic device 301 drives the shelf 201 and the pressing plate to move upward.

[0047] The circulation structure includes a first pipeline 401 and a second pipeline 402. The accommodating portion 103 is connected to the first end of the shelf 201 through the first pipeline 401, and the culture medium or bacterial strain is introduced into the shelf 201 from the first end of the shelf 201. Specifically, the first pipeline 401 is connected to the groove 202. In order to enable the culture medium or bacterial strain to overcome gravity and be introduced into the shelf 201, a liquid pump is arranged on the first pipeline 401. At the same time, in order to facilitate the control of the opening and closing of the first pipeline 401 and each branch, a valve is arranged on the first pipeline 401, and part of the first pipeline 401 is arranged outside the fermentation tank 101 to facilitate the operation of the valve. The accommodating portion 103 is connected to the second end of the shelf 201 through a second pipeline 402, and the second pipeline 402 is connected to the groove 202. The second pipeline 402 reintroduces the culture medium or bacterial strain on the shelf 201 into the accommodating portion 103. In order to facilitate the on-off control of the second pipeline 402 and each branch, a valve is provided on the second pipeline 402, and part of the second pipeline 402 is provided outside the fermentation tank 101 to facilitate the operation of the valve.

[0048] In some examples, a first liquid collecting component 403 is provided on the first pipeline 401, and the first liquid collecting component 403 is hollow and connected to the groove 202. The first pipeline 401 introduces the culture medium or bacterial strain into the first liquid collecting component 403, and then further introduces it into the groove 202, so that the culture medium or bacterial strain is evenly distributed on each layer of the shelf 201; a second liquid collecting component 404 is provided on the second pipeline 402, and the second liquid collecting component 404 is hollow and connected to the groove 202. The culture medium or bacterial strain on the shelf 201 is introduced into the second pipeline 402 through the second liquid collecting component 404, and then further introduced into the accommodating portion 103.

[0049] In some examples, due to the drive of the hydraulic device 301, the height of each shelf 201 in the reaction tank can be changed. In order to ensure that the first pipeline 401 is always connected to each groove 202 and the second pipeline 402 is always connected to each groove 202, the first liquid collecting component 403 is connected with a first soft pipeline 405, the number of the first soft pipeline 405 is the same as the number of the shelves 201, there is a certain height difference between each first soft pipeline 405, and each first soft pipeline 405 is connected to each groove 202. The second liquid collecting component 404 is connected with a second soft pipeline 406, the number of the second soft pipeline 406 is the same as the number of the shelves 201, there is a certain height difference between each second soft pipeline 406, and each second soft pipeline 406 is connected to each groove 202.

[0050] Specifically, in order to ensure the strength and durability of the first soft pipeline 405 and the second soft pipeline 406 , the first soft pipeline 405 and the second soft pipeline 406 are both made of stainless steel braided tubes.

[0051] In some examples, a liquid reservoir 501 is provided in the accommodating portion 103, and the liquid reservoir 501 is used to store bacteria or culture medium, and the liquid reservoir 501 is connected to the first pipeline 401, and the liquid reservoir 501 is connected to the second pipeline 402. When the culture medium or bacteria are introduced into the groove 202 through the first pipeline 401, the absorption structure 203 absorbs the culture medium or bacteria. Since the absorption process of the absorption structure 203 is relatively slow, it is difficult to absorb all the raw materials introduced into the groove 202 into the absorption structure 203 at one time. At this time, the bacteria or culture medium that has not been absorbed flows into the second pipeline 402 along the groove 202 and is temporarily stored in the liquid reservoir 501. The raw materials accumulated in the liquid reservoir 501 are introduced into the groove 202 again through the first pipeline 401, forming a cyclic absorption of the absorption structure 203.

[0052] In some examples, the fermentation tank 101 is provided with an air intake line 502, through which steam or air can flow into the fermentation tank 101 to assist the fermentation reaction. At the same time, steam can be introduced from the air intake line 502 to sterilize the fermentation tank 101. The air intake line 502 is provided with a gas filter, which is used to filter substances in the external gas that are not conducive to the reaction, so as to ensure the efficient reaction.

[0053] In some examples, the imitation solid-state fermentation device also includes a monitoring device, which includes a pressure gauge, a thermometer, and a fermentation monitoring device. The pressure gauge is used to measure the air pressure in the fermentation tank 101. During the fermentation reaction, the fermentation tank 101 needs to have a certain air pressure so that polluted air from the outside cannot enter the tank body to avoid pollution. Specifically, during the reaction, the air pressure in the fermentation tank 101 is maintained at about 0.05Mpa. The thermometer is used to monitor the temperature in the fermentation tank 101, so that the staff can adjust the temperature inside the fermentation tank 101 in real time to make the fermentation reaction efficient. The fermentation monitoring device is used to monitor other parameters about the fermentation reaction in the fermentation tank 101, so that the staff can understand the progress of the fermentation reaction in real time.

[0054] The embodiment of the present invention provides a simulated solid-state fermentation method, comprising:

[0055] The culture medium is added to the accommodating portion 103 , the absorption structure 203 is squeezed, and steam is introduced into the first pipeline 401 . The steam enters the second pipeline 402 through the shelf 201 , and the steam diffuses from the second pipeline 402 to various locations inside the fermentation tank 101 .

[0056] like Figure 5As shown, a fermentation culture medium is added to the accommodating portion 103, and steam is introduced into the first pipeline 401, so that the steam enters the simulated solid-state fermentation device, and then the steam is introduced into the shelf 201 along the first pipeline 401. At this time, the shelf 201 is in a compressed state, and each absorption mechanism is compressed, so that the steam is not easy to escape from the groove 202 of the shelf 201. After passing through the groove 202 of the shelf 201, the steam enters the second pipeline 402, and escapes from the outlet of the second pipeline 402, diffuses to various positions inside the fermentation tank 101, and sterilizes various components in the fermentation tank 101.

[0057] Furthermore, steam is introduced into the air inlet line 502, and the steam sterilizes the air filter and related valves and pipes. A branch of the air inlet line 502 enters the first pipe 401 and merges with the steam in the first pipe 401 for sterilization. The temperature of the sterilization process can be read from a thermometer, and the sterilization pressure can be read from a pressure gauge. Specifically, the sterilization temperature is controlled at about 121°C, and the sterilization time is set to about 30 minutes, or as required.

[0058] The first pipeline 401 continuously introduces the culture medium into the groove 202, releasing the absorption structure 203, and the absorption structure 203 absorbs the culture medium. The unabsorbed culture medium flows back to the accommodating portion 103 along the second pipeline 402, so that the absorption structure 203 circulates and absorbs the culture medium.

[0059] like Figure 6 As shown, the pressure plate is pulled by the hydraulic device 301, and the pressure plate pulls each shelf 201 upward, and the compressed absorption structure 203 is released, and at the same time absorbs the culture medium in the groove 202 of each shelf 201, and is in a saturated state. At this time, the unabsorbed culture medium flows back to the accommodating portion 103. Since the absorption structure 203 is not under pressure, a small amount of culture medium will escape from the groove 202 of the shelf 201 and drip on the bottom of the reaction tank, that is, drip on the accommodating portion 103. The culture medium is further introduced into the shelf 201, so that the absorption structure 203 performs cyclic absorption, and air and culture medium are continuously transported to the shelf 201, that is, continuously transported to the absorption structure 203. During the absorption process, constant temperature air is introduced into the reaction tank to control the temperature of the fermentation tank 101.

[0060] Specifically, two branches are provided on the air inlet line 502, one branch is provided with a one-way valve, and the other branch is provided with a two-way valve. After the sterilization is completed, the one-way valve is closed first. Since the flow direction of the one-way valve is from top to bottom, that is, the external gas can pass through the one-way valve, and the internal gas cannot be discharged from the one-way valve, the culture medium from bottom to top cannot pass through the one-way valve. Even if it is operated incorrectly, the culture medium will not flow out from the air filter. However, for the sake of safety, the one-way valve should be closed first. Compressed air is introduced from the air inlet line 502, and the compressed air enters the fermentation tank 101 from the two-way valve to maintain the pressure of the device. The pressure is maintained at about 0.05MPa to avoid the device from losing pressure and prevent the outside air from entering the device to cause microbial contamination.

[0061] During the absorption process, the one-way valve is gradually opened and the two-way valve is closed, allowing air to enter the first pipeline 401 to mix with the culture medium and enter the shelf 201 together, escaping from the groove 202 in the shelf 201 to the holes of the absorption structure 203.

[0062] The temperature inside the fermentation tank 101 is lowered, and the bacteria are connected from the first pipeline 401 and introduced into the groove 202. The absorption structure 203 absorbs the bacteria, and the unabsorbed bacteria flow back to the containing part 103 along the second pipeline 402. The bacteria are further circulated and introduced into the groove 202, and the absorption structure 203 circulates and absorbs the bacteria.

[0063] After the sterilization operation is completed, when the temperature is lowered to a suitable fermentation temperature, the bacteria are introduced into the first pipeline 401, and the bacteria are transported toward the shelf 201 and refluxed into the accommodating portion 103. After continuous introduction of the bacteria, the bacteria will eventually be evenly distributed in the culture medium. At this time, the hydraulic device is started to compress the absorption structure 203, and then the hydraulic device 301 moves upward to release the compressed absorption structure 203. Repeating the operation multiple times can evenly distribute the bacteria on the absorption structure 203 for fermentation operation, while continuously providing culture medium to the absorption structure 203.

[0064] like Figure 7 As shown, constant temperature air is continuously introduced into the fermentation tank 101 to provide oxygen for fermentation, remove waste gas, and maintain a stable fermentation temperature.

[0065] After the fermentation products accumulate, the absorption structure 203 is squeezed, and the fermentation products flow to the receiving portion 103 and are discharged from the fermentation tank 101 .

[0066] like Figure 8As shown, in the absorption structure 203, after the fermentation product generated by fermentation accumulates to a certain amount, the absorption structure 203 is compressed to allow the fermentation product to accumulate at the bottom of the fermentation tank 101. The compressed absorption structure 203 is released, so that the absorption structure 203 absorbs the culture medium and continues to ferment. This cycle is repeated until the fermentation product accumulates to a certain amount at the bottom of the device, and the fermentation product is discharged. The fermentation product remaining on the shelf 201, the pipeline and the receiving portion 103 can be sucked through the first pipeline 401, so that the remaining fermentation product can be accumulated in the receiving portion 103, and finally discharged and collected.

[0067] At the same time, the fermentation tank 101 is provided with a sight glass, through which the fermentation conditions in the fermentation tank 101 can be observed. In addition, the fermentation detection device can also monitor the fermentation parameters, such as pH, dissolved oxygen content, etc.

[0068] In the description of this specification, if the reference terms "one embodiment", "some examples", "some embodiments", "illustrative embodiment", "example", "specific example", or "some examples" appear, it means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0069] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A simulated solid-state fermentation device, characterized in that: include: A fermentation tank, wherein the internal space of the fermentation tank includes a reaction part and a receiving part, the reaction part is communicated with the receiving part, and the reaction part is arranged above the receiving part; A shelf is provided in the reaction part, a groove is provided on the surface of the shelf, a culture medium or bacterial strain can flow along the groove, an absorption structure is provided above the shelf, and the absorption structure is used to absorb the culture medium or bacterial strain inside; a plurality of shelves are provided, each of the shelves is longitudinally distributed in the reaction part, there is a height difference between adjacent shelves, adjacent shelves are connected by connecting rods, and each of the shelves can slide on the connecting rods to change the height of the shelves; A hydraulic device, the hydraulic device is connected to the shelves, the hydraulic device can push the shelves to reduce the distance between the shelves, the accommodating portion is provided with a supporting structure, the supporting structure is arranged below the shelves, and the supporting structure is used to support the shelves; A circulation structure, wherein the circulation structure comprises a first pipeline and a second pipeline, wherein the accommodating portion is connected to the first end of the shelf through the first pipeline, and the first pipeline is connected to the groove, and the accommodating portion is connected to the second end of the shelf through the second pipeline, and the second pipeline is connected to the groove.

2. The simulated solid-state fermentation device according to claim 1, characterized in that: The shelf is provided with a plurality of the grooves, each of which is arranged in parallel, and each of the grooves is connected in sequence from end to end.

3. The simulated solid-state fermentation device according to claim 2, characterized in that: The first pipeline is provided with a first liquid collecting component, the first liquid collecting component is hollow and connected to the groove, and the second pipeline is provided with a second liquid collecting component, the second liquid collecting component is hollow and connected to the groove.

4. The simulated solid-state fermentation device according to claim 3, characterized in that: The first liquid collecting component is connected to a first soft pipe, the first soft pipe is connected to the groove, and the number of the first soft pipes is the same as the number of the shelves. The second liquid collecting component is connected to a second soft pipe, the second soft pipe is connected to the groove, and the number of the second soft pipes is the same as the number of the shelves.

5. The simulated solid-state fermentation device according to claim 1, characterized in that: A liquid storage tank is disposed in the accommodating portion, the liquid storage tank is communicated with the first pipeline, and the liquid storage tank is communicated with the second pipeline.

6. The simulated solid-state fermentation device according to claim 3, characterized in that: The fermentation tank is provided with an air intake pipeline, a gas filter is provided on the air intake pipeline, and the air intake pipeline is connected to the first liquid collecting component.

7. The simulated solid-state fermentation device according to claim 1, characterized in that: A simulated solid-state fermentation device also includes a monitoring device, which includes a pressure gauge, a thermometer, and a fermentation monitoring device. The pressure gauge is arranged on the fermentation tank, and the thermometer and the fermentation monitoring device are arranged on the first pipeline.

8. A simulated solid-state fermentation method, using the simulated solid-state fermentation device according to any one of claims 1 to 7, characterized in that: Adding culture medium into the accommodating part, squeezing the absorption structure, introducing steam into the first pipeline, the steam enters the second pipeline through the shelf, and the steam diffuses from the second pipeline to various positions inside the fermentation tank; The first pipeline continuously introduces the culture medium into the groove, releases the absorption structure, and the absorption structure absorbs the culture medium. The unabsorbed culture medium flows back to the containing part along the second pipeline, so that the absorption structure circulates and absorbs the culture medium. Lowering the temperature inside the fermentation tank, connecting the bacteria from the first pipeline, introducing the bacteria into the groove, the absorption structure absorbs the bacteria, and the unabsorbed bacteria flow back to the accommodation part along the second pipeline, and further circulate the bacteria into the groove, and the absorption structure circulates and absorbs the bacteria; After the fermentation product accumulates, the absorption structure is squeezed and the fermentation product flows to the receiving part, thereby being discharged from the fermentation tank.

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