A combination of intestinal microbial gene markers for the identification of type 2 diabetes and its application

Abstract

The present invention belongs to the technical field of microbial medicine, and particularly relates to a combination of gut microbial gene markers for the identification of type 2 diabetes and its application, especially a combination of gut microbial gene markers for differentiating diabetic patients or patients at high risk of diabetes from the normal population. The gut microbial gene marker combination described in the present invention consists of a total of 246 different OTUs, and is a gut microbial marker model that can distinguish diabetic patients or patients at high risk of diabetes from the normal population. By using these 246 differential OTUs and constructing an ROC curve, a marker combination for non-invasive screening of diabetic populations with both high sensitivity and specificity was successfully obtained, and a discrimination model with both high sensitivity and specificity was further established, which can effectively distinguish diabetic patients from healthy populations, showing good discrimination ability.

Description

Technical Field

[0001] The present invention belongs to the field of microbial medicine technology, and specifically relates to a combination of intestinal microbial gene markers for identifying type 2 diabetes, especially a combination of intestinal microbial gene markers for distinguishing diabetic patients or high-risk diabetic patients from normal people. Background Art

[0002] Diabetes is one of the most common metabolic diseases in the world, affecting approximately 400 million people worldwide. Diabetes is primarily diagnosed by measuring blood glucose. However, this method can be subject to haphazard testing, including instrument deviations and operator mishandling, leading to failure to diagnose patients in the early stages of or at high risk of developing diabetes. Furthermore, some patients with coagulation disorders or other contraindications cannot undergo blood sampling. Therefore, there is an urgent need to develop safer, highly sensitive, and specific methods for predicting or diagnosing diabetes.

[0003] The human intestine is colonized by a huge number of intestinal bacteria (about 10 trillion), with rich species and complex structures. According to research, the composition and homeostasis of the intestinal flora are closely related to human health, and have a significant impact on the host's digestion and absorption, metabolic function, immune response, etc. According to existing research reports, the intestinal flora is closely related to many diseases, such as rectal cancer, cirrhosis, stem cell liver cancer, diabetes, etc. Based on the strong potential of the intestinal flora, many studies have shown that the intestinal flora may have the ability to become a new way to diagnose diseases. For example, Chinese patent CN105378739A discloses a model that uses metagenomic clusters (MGCs) to identify individuals with or at risk of developing type 2 diabetes. However, due to the complex and time-consuming process of metagenomic sequencing, and the large number of bacterial genera targeted by sequencing, the specificity of the marker is not high. Therefore, there is an urgent need to develop a gut microbial gene marker with high sensitivity and specificity for clinical rapid and accurate identification and screening of diabetic patients. Summary of the Invention

[0004] To this end, the technical problem to be solved by the present invention is to provide a combination of intestinal microbial gene markers for the identification of type 2 diabetes, which includes 246 OTUs that show statistical differences between normal and diabetic groups and can be used for the clinical screening and diagnosis of diabetic patients;

[0005] The second technical problem to be solved by the present invention is to provide the application of the intestinal microbial gene markers in the clinical screening and diagnosis of patients with type 2 diabetes.

[0006] To solve the above technical problems, the present invention provides a combination of intestinal microbial gene markers for the identification of type 2 diabetes, comprising a combination of 246 OTUs as shown in the following table:

[0007]

[0008]

[0009]

[0010]

[0011] Preferably, each of the OTU markers includes a nucleotide sequence structure as shown in the following table:

[0012]

[0013]

[0014]

[0015] The present invention also discloses the use of the microbial gene marker combination in preparing a diagnostic reagent for differentiating diabetic patients from healthy people, or for screening or auxiliary diagnosis of diabetic patients, or for preparing a diabetic microbial targeted therapeutic drug.

[0016] Specifically, the diabetes includes type 2 diabetes.

[0017] The present invention also discloses a kit for identifying and diagnosing diabetes, comprising primers of the intestinal microbial gene marker combination.

[0018] Specifically, the kit includes primers 341F_805R, with an insertion length of 465bq and a sequencing length of 2*300bp. The specific structure is:

[0019] Upstream primer: 341F 5′-CCTACGGGNGGCWGCAG-3′;

[0020] Downstream primer: 805R5′-GACTACHVGGGTATCTAATCC-3′.

[0021] The present invention also discloses a method for constructing a model for predicting and assisting in the diagnosis of diabetes, comprising the following steps:

[0022] (1) Stool samples from diabetic and healthy subjects meeting the requirements were collected to complete 16S rRNA gene sequencing;

[0023] (2) The obtained gene sequences were clustered into OTUs and their abundance was determined based on a similarity of 97%. The RDP classifier Bayesian algorithm was then used to perform taxonomic analysis on the OTU representative sequences at the 97% similarity level, and the community composition of each sample was statistically analyzed at each taxonomic level.

[0024] (3) Based on the results of step (2), statistically significant OTUs that can accurately distinguish diabetic nephropathy from normal subjects are screened out through statistical analysis;

[0025] (4) Using the OTU results screened in step (3), calculate the OTUs that are enriched and statistically significant in the diabetic and normal groups, and add the two types of OTUs together; use the total difference between the two groups of OTUs to construct an ROC curve to determine the ability of this OTU combination marker to distinguish between diabetic and normal people.

[0026] Specifically, the method for constructing a model for predicting and assisting in the diagnosis of diabetes further includes the steps of performing genetic testing on the feces of the patient to be diagnosed according to the microbial gene markers described in claim 1 or 2, and the step of verifying the diagnosis results.

[0027] Specifically, among the 246 OTU compositions, 56 were increased in diabetic patients and 190 were decreased in diabetic patients.

[0028] The present invention also discloses a composition for microbial targeted treatment of diabetes, comprising the microbial gene marker combination as described in claim 1 or 2, that is, treating or controlling diabetes by increasing or decreasing the relative content of the corresponding markers, performing early intervention in diabetes, thereby controlling the condition of diabetes and preventing the occurrence of complications.

[0029] The present invention further provides a new approach for treating diabetes, namely, by changing or controlling the intestinal flora through flora transplantation or the use of related drugs, thereby achieving the purpose of treating diabetes and controlling the progression of diabetes.

[0030] The present invention can further be used as a verification method for patients with diabetes. Some normal people may be misdiagnosed with diabetes due to improper diet, improper measurement methods, etc. In this case, the present invention can be used to verify the correctness of the original diagnosis.

[0031] The intestinal microbial gene marker combination described in this invention, consisting of 246 distinct OTUs, is a gut microbial marker model that can distinguish between patients with diabetes or those at high risk of diabetes and healthy controls. 16S rRNA sequencing of patient samples revealed 246 of the 3,115 OTUs screened to be statistically different between the healthy and diabetic groups. Using these 246 differentially expressed OTUs and constructing receiver operating characteristic (ROC) curves, a noninvasive screening marker combination with high sensitivity and specificity for diabetic patients was successfully developed. Furthermore, a highly sensitive and specific discrimination model was established, effectively distinguishing patients with diabetes from healthy controls, demonstrating excellent discrimination capabilities. Based on these differentially expressed microbiota, diabetic patients who may be normoglycemic or at high risk for diabetes can be screened and identified from the healthy population.

[0032] The proposed method can be used primarily to differentiate patients with type 2 diabetes (DM) from healthy controls. 16S rRNA gene sequencing is used to analyze and identify the microbial profiles of the intestinal flora of the screened population. Further statistical analysis identifies distinct bacterial communities at the OTU level. This proposed model, which uses intestinal flora markers to predict the presence or absence of diabetes or a high risk of diabetes in individuals, can be widely used for diabetes screening in healthy individuals. Especially for many patients who have contraindications to blood tests, such as those taking large amounts of anticoagulants or those with coagulation disorders such as coagulation factor deficiency, the present invention can be used for non-invasive auxiliary diagnosis of diabetes; it can effectively avoid the situation where, when using a blood glucose meter for examination, patients who actually have diabetes are not screened out due to problems such as equipment aging or improper operation by the operator, and it is more stable than the blood glucose concentration in the blood. It is suitable for all normal people and is more suitable for patients with bleeding tendencies or contraindications to blood tests; compared with conventional blood tests, the present invention is completely non-invasive; at the same time, since the intestinal flora is a relatively stable composition and changes over a relatively long period of time, there will be no extreme result deviation, the sensitivity and specificity are both highly specific, and the results are true and reliable. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to make the content of the present invention more clearly understood, the present invention is further described in detail below based on specific embodiments of the present invention in conjunction with the accompanying drawings, wherein:

[0034] Figure 1 A flowchart of the model for predicting and assisting in the diagnosis of diabetes according to the present invention;

[0035] Figure 2 The obtained ROC curve analysis results are shown in Figure 3. DETAILED DESCRIPTION

[0036] Unless otherwise specified, the methods used in the following examples are all conventional methods. The materials or reagents required in the following examples were all obtained from public commercial channels unless otherwise specified.

[0037] Example 1

[0038] The fecal microbial composition of 137 patients with type 2 diabetes and 179 healthy controls was determined. The subjects were randomly divided into a discovery cohort (102 patients with diabetes and 131 healthy controls) and a validation cohort (35 patients with diabetes and 48 healthy controls).

[0039] like Figure 1 The flowchart shown in the figure is to establish a microbial differentiation model between diabetic patients and healthy people in the discovery cohort based on high-throughput sequencing data.

[0040] Sample Collection: This study protocol was approved by the Ethics Committee of the First Affiliated Hospital of Zhengzhou University, in accordance with the design principles of a prospective clinical trial. After patients signed informed consent, fresh stool samples were collected early in the morning of the second day of hospitalization. After collection, they were placed in an ice box with ice packs and transported to the Zhengzhou University Biobank within half an hour. Upon arrival, they were immediately stored in a -80°C freezer to avoid repeated freezing during transportation. All other steps except this were completed using the Shanghai Illumina platform. A total of 137 stool samples were collected from patients with type 2 diabetes and 179 healthy controls. A discovery cohort of 234 individuals (102 with diabetes and 131 with normal controls) was used for model development and validation, and a validation cohort of 83 individuals (35 with diabetes and 48 with normal controls) was used for model validation.

[0041] Total microbial DNA was extracted from samples collected from the discovery cohort and 16S rDNA Miseq sequencing of the intestinal flora was performed. The DNA extraction procedure specifically included the following steps:

[0042] (1) Chemical lysis of cell membranes and nuclear membranes: add 790 μl of lysis buffer (4 mmol / L guanidine thiocyanate, 250 μl; 10% N-lauroylsarcosine, 40 μl; 5% N-lauroylsarcosine, 0.1 mmol / L PBS buffer [pH 8.0], 500 μl) to each sample, vortex vigorously, and incubate at 70°C for 1 h.

[0043] (2) Physical beating method (Beads-Beating) to fully lyse the cell membrane and nuclear membrane: After the incubation, add glass beads (0.1 mm, 500-750 μl) and mix, and beat for 10 min (25 Hz / s);

[0044] (3) The subsequent extraction was carried out according to the instructions of the extraction kit (EZNA DNA Kit);

[0045] (4) After the first centrifugation (14000g, 3 min), the supernatant was placed in a 2 ml centrifuge tube, 200 μl of SP2 buffer was added, vortexed, and placed in an ice bath for 5 min.

[0046] (5) Centrifuge for the second time (14000g, 3min). Take the supernatant and place it in a new 2ml centrifuge tube. Add 200ul of cHTRReagent (shake well before use), mix well and let it stand at room temperature for 2min.

[0047] (6) After the third centrifugation (14000g, 2min), 500ul of the supernatant was placed in a new 2ml centrifuge tube, 20ul of proteinase K (20mg / ml) and 500ul of BL buffer were added, vortexed and mixed, and incubated at 70℃ for 10min; after incubation, 500ul of anhydrous ethanol was added, vortexed and passed through the column: Insert the DNA mini column into a 2 mL collection tube.

[0048] The 16S rRNA gene was amplified using the DNA extracted from each sample as a template. The sequencing primer sequences included:

[0049] Upstream primer: 341F 5′-CCTACGGGNGGCWGCAG-3′;

[0050] Downstream primer: 805R5′-GACTACHVGGGTATCTAATCC-3′.

[0051] The PCR amplification and sequencing operations specifically include the following steps:

[0052] The V3-V4 region of the 16S rRNA gene was amplified using the DNA extracted from each sample as a template. The reaction conditions were as follows:

[0053]

[0054] The PCR products were mixed in the same ratio and sequenced on the Illumina Miseq platform.

[0055] Example 2 OTU clustering and annotation

[0056] After high-throughput sequencing of 16S rRNA, the sequences were classified and divided into many operational units according to their similarity, namely an OTU (Operational Taxonomy Unit). All sequences can be divided into OTUs according to different similarity levels. Usually, OTUs at a similarity level of 97% are used for bioinformatics statistical analysis (Usearch software). OTU is used as the basic operational unit and the RDP classifier based on Silva (SSU123) 16S rRNA data is used ( http: / / rdp.cme.msu.edu / ) for analysis and annotation. The RDP classifier Bayesian algorithm was used to perform taxonomic analysis on the OTU representative sequences at a similarity level of 97%, thereby obtaining the species classification information corresponding to each OTU. The community composition of each sample was statistically analyzed at each classification level: domain, kingdom, phylum, class, order, family, genus, and species.

[0057] Community composition difference analysis: We used LEfSe analysis (Linear discriminant analysis Effect Siz, linear discriminant analysis) to evaluate the difference in abundance of all OTUs between the two groups, and obtained all OTUs with LDA>2.0 (Linear discriminant analysis). A total of 246 OTUs had LDA values ​​greater than 2.0, of which 56 were enriched in diabetic patients and 190 were more enriched in normal people. According to the identified bacteria, these OTUs belong to 5 different phylums, 10 different classes, 10 different orders, 21 different families, 41 different genus, and 3 unclassified bacteria.

[0058] The test showed that the increase or decrease of different OUT in diabetic patients is shown in Table 1 below.

[0059] Table 1 Sequence structure and changes of OTU

[0060]

[0061]

[0062]

[0063]

[0064]

[0065]

[0066] As shown in the table above, the OTUs marked CON are more enriched in CON, that is, they are reduced in diabetes; the OTUs marked DM are increased in diabetic patients.

[0067] Example 3 ROC analysis

[0068] The measurement results of the diabetic population and the normal population were analyzed. Based on statistical knowledge, a total of 246 different OTUs with LDA values ​​greater than 2.0 were divided into a disease-enriched group and a normal population-enriched group. The number of OTUs in the two groups of the two populations was added together, and the two groups were subtracted. The results of the subtraction of the two OTUs were then summed. The calculation method is shown in Table 2 below.

[0069] Table 2 Calculation method

[0070]

[0071] Using the upper and lower limits of the difference obtained for each sample, the class interval, and the cutoff point (threshold, cut-off point), a cumulative frequency distribution table is compiled according to the selected class interval, and the sensitivity (Sensitivity), specificity, and false-positive rate (1-Specificity) for all cutoff points are calculated. The ROC curve is plotted, with sensitivity as the vertical axis representing the true-positive rate and (1-Specificity) as the horizontal axis representing the false-positive rate. The area under the ROC curve (AUC) is between 1.0 and 0.5, and the closer it is to the upper left corner, the higher the diagnostic accuracy. When AUC is greater than 0.5, the closer the AUC is to 1, the better the diagnostic effect. An AUC of 0.5-0.7 has low accuracy, an AUC of 0.7-0.9 has some accuracy, and an AUC above 0.9 has high accuracy. Therefore, the closer the ROC curve is to the upper left corner, the higher the accuracy of the test. The point closest to the upper left corner of the ROC curve is the best threshold with the least error, that is, the cut-off value, with the least total number of false positives and false negatives. At this point, the combined level of sensitivity and specificity of the model is the highest, that is, the diagnostic efficiency of the model is the best.

[0072] Construct the ROC model and calculate the area under the curve AUC (see Figure 2 In the discovery cohort, the AUC reached 92.25% (95% confidence interval: 88.71%-95.79%). At the optimal cutoff, the model's sensitivity and specificity reached 75.81% and 92.22%, respectively. This demonstrates the high diagnostic efficacy of this discrimination model.

[0073] The relative abundance values ​​of the 246 OTUs required for inclusion in the present invention were measured, and the model established by the present invention was used to test whether the subjects had diabetes or were at high risk of diabetes.

[0074] Experimental Example 4

[0075] The present invention is further illustrated by taking the verification queue as an experimental example.

[0076] We validated the model in a validation cohort of 83 individuals, including 35 diabetic patients and 48 healthy individuals. This cohort included the model and validation. Fecal samples were collected from the participants, total microbial DNA was extracted, and 16S rDNA Miseq sequencing of the intestinal flora was performed. The relative abundance values ​​of the 246 OTUs required for the model were identified and calculated, and then incorporated into the model for identification. Specifically, the following were included:

[0077] (1) According to the design principles of prospective clinical trials, such as the present invention Figure 1 The study protocol was approved by the Ethics Committee of the First Affiliated Hospital of Zhengzhou University, and all enrolled patients signed informed consent for the study protocol and informed consent for clinical sample collection.

[0078] (2) Fecal samples were collected from the enrolled subjects (diabetic patients and healthy people), and the total microbial DNA in the fecal samples was extracted according to the standard DNA extraction method. The 16S rDNA high-throughput sequencing of the intestinal flora was completed on the Illumina MiSeq platform;

[0079] (3) Based on the intestinal flora sequencing data, the intestinal flora of 83 subjects in the validation cohort were classified at the 16S rDNA gene sequence classification unit (Operational Taxonomy Unit, OTU) level, and the relative abundance of 246 OTUs required by the model was calculated;

[0080] (4) The relative abundance of 246 microbial gene markers required for the validation cohort was obtained. According to the model, the 56 OTUs that were more enriched in diabetic patients and the 190 OTUs that were more enriched in normal subjects were added together. The total difference between the two groups of OTUs in the two groups of people was calculated and incorporated into the model established based on the discovery cohort to test the model's discriminative ability.

[0081] (5) Draw the ROC curve of the validation cohort group (such as Figure 2In Figure 3B), the identification effect of the model was evaluated, and the area under the curve (AUC) reached 90.48%. That is, the model had a discrimination ability of 90.48% for 35 diabetic patients and 48 normal subjects in the validation cohort, and the sensitivity and specificity reached 90.91% and 78.72%, respectively, verifying the excellent identification ability of the identification model described in the present invention.

[0082] Therefore, the microbial gene discrimination model of the present invention achieves good discrimination ability between type 2 diabetes patients and healthy people.

[0083] While the present invention is described through the above-described embodiments, the present invention is not limited to the above-described detailed methods, nor does it necessarily rely on the above-described detailed methods for implementation. Those skilled in the art will appreciate that any improvements to the present invention, equivalent replacements for raw materials in the products of the present invention, addition of auxiliary ingredients, and selection of specific methods, etc., fall within the scope of protection and disclosure of the present invention. Sequence Listing <110> The First Affiliated Hospital of Zhengzhou University <120> A combination of intestinal microbial gene markers for identifying type 2 diabetes and its application <160> 246 <170> SIPOSequenceListing 1.0 <210> 1 <211> 440 <212> DNA <213> OTU11 <400> 1 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180 aagcgttgtc cggatttact gggtgtaaag ggtgcgcagg cggaaggcta agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 2 <211> 440 <212> DNA <213> OTU36 <400> 2 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgtgtc cggaattact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgggtagtc 440 <210> 3 <211> 440 <212> DNA <213> OTU41 <400> 3 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgagta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgaggca agtctgatgt 240 gaaagcctgg ggcttaaccc cggaactgca ttggaaactg cttgctgga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 4 <211> 460 <212> DNA <213> OTU58 <400> 4 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg ttagcctgaa ccagccaagt 60 agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatataagaa taaagtgcag 120 tatgtatact gttttgtatg tattatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggactggta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 tcagcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 5 <211> 440 <212> DNA <213> OTU66 <400> 5 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt 240 gaaatccccg ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga 300 ggggggtaga attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg 360 cgaaggcggc cccctggacg aagactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac ccgagtagtc 440 <210> 6 <211> 440 <212> DNA <213> OTU72 <400> 6 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attgggaaga acaccagtgg 360 cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 7 <211> 440 <212> DNA <213> OTU77 <400> 7 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg atgaagtatt tcggtatgta aaactctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggtatggca agtcagaagt 240 gaaaacccag ggcttaactc tgggactgct ttgaaactg tcagactgga gtgcaggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 8 <211> 440 <212> DNA <213> OTU101 <400> 8 cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgcagca agtctgatgt 240 gaaaggcagg ggcttaaccc ctggactgca ttggaaactg ctgtgcttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 9 <211> 440 <212> DNA <213> OTU104 <400> 9 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatc tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggtatggca agtctgatgt 240 gaaaggccgg ggctcaaccc cgggactgca ttggaaactg tcacacttga gtgtcggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg acaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 10 <211> 440 <212> DNA <213> OTU107 <400> 10 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagccccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggtatggca agtcagaagt 240 gaaagcctgg ggctcaaccc cggaattgct tttgaaactg tcaaactaga gtgtcggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggtgg 360 cgaaggcggc ttactggacg accactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgggtagtc 440 <210> 11 <211> 441 <212> DNA <213> OTU110 <400> 11 cctacgggtg gctgcagtgg ggaatattgc gcaatggggg aaaccctgac gcagcaacgc 60 cgcgtgattg aagaaggcct tcgggttgta aagatcttta attcgggacg aattttgacg 120 gtaccgaaag aataagctcc ggctaactac gtgccagcag ccgcggtaat acgtagggag 180 caagcgttat ccggatttac tgggtgtaaa gggcgcgcag gcgggccggc aagttggaag 240 tgaaatccgg gggcttaacc cccgaactgc tttcaaaact gctggtcttg agtgatggag 300 aggcaggcgg aattccgtgt gtagcggtga aatgcgtaga tatacggagg aacaccagtg 360 gcgaaggcgg cctgctggac attaactgac gctgaggcgc gaaagcgtgg ggagcaaaca 420 ggattagata ccctagtagt c 441 <210> 12 <211> 440 <212> DNA <213> OTU111 <400> 12 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctgctggact gttactgaca ctgaggcacg aaagcgtggg gagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 13 <211> 441 <212> DNA <213> OTU124 <400> 13 cctacgggcg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgatgc 60 cgcgtgaggg aagaaggttt taggattgta aacctctgtc tttggggacg aaaaatgacg 120 gtacccaagg aggaagctcc ggctaactac gtgccagcag ccgcggtaat acgtagggag 180 cgagcgttgt ccggaattac tgggtgtaaa gggagcgtag gcgggatcgc aagtcagatg 240 tgaaaactat gggcttaacc cataaactgc atttgaaact gtggttcttg agtgaagtag 300 aggtaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg aacatcagtg 360 gcgaaggcgg cttactggac tgtaactgac actgaggctc gaaagcgtgg ggagcaaaca 420 ggattagata cccgagtagt c 441 <210> 14 <211> 440 <212> DNA <213> OTU147 <400> 14 cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgaggg aagaaggttt tcggattgta aacctctgtt cttagtgacg ataatgacgg 120 tagctaagga gaaagctccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180 gagcgtgtc cggatttact gggtgtaaag ggtgcgtagg cggcgaggca agtcaggcgt 240 gaaatctatg ggcttaaccc ataaactgcg cttgaaactg tcttgcttga gtgaagtaga 300 ggtaggcgga attcccggtg tagcggtgaa atgcgtagag atcgggagga acaccagtgg 360 cgaaggcggc ctactgggct ttaactgacg ctgaagcacg aaagcatggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 15 <211> 440 <212> DNA <213> OTU149 <400> 15 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 16 <211> 440 <212> DNA <213> OTU200 <400> 16 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cggaggatcc 180 gagcgttatc cggatttatt gggtttaaag ggagcgtagg cgggttgtta agtcagttgt 240 gaaagtttgc ggctcaaccg taaattgca gttgatactg gcgaccttga gtgcaacaga 300 ggtaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360 cgaaggcagc tcactagact gcaactgaca ctgatgctcg aaagtgtggg tatcaaacag 420 gattagatac ccctgtagtc 440 <210> 17 <211> 440 <212> DNA <213> OTU232 <400> 17 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggttttgca agtctgaagt 240 gaaagcccgg ggcttaaccc cgggactgct ttggaaactg taggactaga gtgcaggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 18 <211> 460 <212> DNA <213> OTU235 <400> 18 cctacgggtg gctgcagtga ggaatattgg tcaatggccg agaggctgaa ccagccaagt 60 cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgcgg 120 gacgtgtccc gttttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240 cggcctttta agtcagcggt gaaagtctgt ggctcaacca tagaattgcc gttgaaactg 300 gggggcttga gtatgtttga ggcaggcgga atgcgtggtg tagcggtgaa atgcatagat 360 atcacgcaga accccgattg cgaaggcagc ctgccaagcc attactgacg ctgatgcacg 420 aaagcgtggg gatcaaacag gattagatac cctagtagtc 460 <210> 19 <211> 440 <212> DNA <213> OTU245 <400> 19 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggaattact gggtgtaaag ggtgcgtaga cggcaaggca agtctgatgt 240 gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagag attaggaaga acaccggtgg 360 cgaaggcgac tttctggacg aaaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 20 <211> 440 <212> DNA <213> OTU266 <400> 20 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggaattact gggtgtaaag ggtgcgtagg tggtatggca agtcagaagt 240 gaaaacccag ggcttaactc tgggactgct ttgaaactg tcagactgga gtgcaggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acatcagtgg 360 cgaaggcggc ttactggact gaaactgaca ctgaggcacg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 21 <211> 440 <212> DNA <213> OTU276 <400> 21 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cgggagaaca agttggaagt 240 gaaatccatg ggctcaaccc atgaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctgctggact gttactgaca ctgaggcacg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 22 <211> 442 <212> DNA <213> OTU277 <400> 22 cctacgggtg gctccagtgg ggaatattgc acaatgggcg aaagcctgat gcagcaacgc 60 cgcgtgagg aagacggttt tcggattgta aacctctgtt ttcggtgacg aacaaatgac 120 ggtaaccgag taggaagcca cggctacta cgtgccagca gccgcggtaa tacgtaggtg 180 gcaagcgtta tccggaatta ctgggtgtaa agggagcgca ggcgggatag caagtcagct 240 300 gaggcaagcg gaattccgag tgtagcggtg aaatgcgtag atattcggag gaacaccagt 360 ggcgaaggcg gcttgctggg ctctaactga cgctgaggct cgaaagtgtg gggagcaaac 420 aggattagat accctagtag tc 442 <210> 23 <211> 465 <212> DNA <213> OTU286 <400> 23 cctacgggag gctgcagtgg ggaatattgc acatggggcg caagcctgat gcagccatgc 60 cgcgtgtgtg aagaaggcct tcggttgta aagcactttc agcggggagg aaggcgttaa 120 ggttaataac cttggcgatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtc tgtcaagtcg gatgtgaat ccccgggctc aacctgggaa ctgcatctga 300 tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gatacccctg tagtc 465 <210> 24 <211> 443 <212> DNA <213> OTU344 <400> 24 cctacgggtg gcagcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60 cgcgtgaagg agaaggctt tcggttgta aacttcttt gtcagggacg aagcaagtga 120 cggtacctga cgaataagcc acggctaact acgtgccagc agccgcggta atacgtaggt 180 ggcaagcgtt atccggattt actgggtgta aagggcgtgt aggcgggaaa gcaagtcaga 240 tgtgaaaact gtgggctcaa cccacagcct gcatttgaaa ctgtttttct tgagtactgg 300 agaggcagat ggaattccta gtgtagcggt gaaatgcgta gatattagga ggaacaccag 360 tggcgaaggc gatctgctgg acagcaactg acgctgaggc gcgaaagcgt ggggagcaaa 420 caggattaga taccctagta gtc 443 <210> 25 <211> 460 <212> DNA <213> OTU366 <400> 25 cctacgggtg gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300 gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 460. aaagtgtggg tatcaaacag gattagatac ccgagtagtc <210> 26 <211> 447 <212> DNA <213> OTU368 <400> 26 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc cgcgtgcggg atgacggcct tcggggttgta aaccgctttt gatcgggagc aagccttcgg 120 gtgagtgtac ctttcgaata agcaccggct aactacgtgc cagcagccgc ggtaatacgt agggtgcaag cgttatccgg aattattggg cgtaaagggc tcgtaggcgg ttcgtcgcgt 240 ccggtgtgaa agtccatcgc ttaacggtgg atctgcgccg ggtacggggcg ggctggagtg cggtagggga gactggaatt cccggtgtaa cggtggaatg tgtagatc gggaagaaca ccaatggcga aggcaggtct ctgggccgtt actgacgctg aggagcgaaa gcgtggggag 420 cgaacaggat ownerccct agtagtc <210> 27 <211> 440 <212> DNA <213> OTU386 <400> 27 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaatggca agtctgatgt 240 gaaaggccgg ggctcaaccc cgggactgca ttggaaactg ccaatctaga gtaccggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagctaggg tagcgaacgg 420 gattagatac cccagtagtc 440 <210> 28 <211> 460 <212> DNA <213> OTU405 <400> 28 cctacggggg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagttttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420 aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460 <210> 29 <211> 439 <212> DNA <213> OTU418 <400> 29 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttatc cggatttatt gggtgtaaag ggcgcgcagg cgggccggca agttggaagt 240 gaaatccggg ggcttaaccc ccgaactgct ttcaaaactg ctggtcttga gtgatggaga 300 ggcaggcgga attccgtgtg tagcggtgaa atgcgtagat atacggagga acaccagtgg 360 cgaaggcggc ctgctggaca ttaactgacg ctgatgctcg aaagtgtggg tatcaaacag 420 gattagatcc cttgtagtc 439 <210> 30 <211> 442 <212> DNA <213> OTU427 <400> 30 cctacgggag gcagcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60 cgcgtgaagg aagaaggctt tcgggttgta aacttctttt aagaggggaag agcagaagac 120 ggtaccctt gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180 gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccgggtgcg caagtcagat 240 gtgaaatctc agggctcaac cctgaaactg catttgaaac tgtgcatctt gagtgccgga 300 gaggtaatcg gaattccttg tgtagcggtg aaatgcgtag atataaggaa gaacaccagt 360 ggcgaaggcg gattactgga cggtaactga cggtgaggcg cgaaagcgtg gggagcaaac 420 aggattagat acccgggtag tc 442 <210> 31 <211> 440 <212> DNA <213> OTU428 <400> 31 cctacgggag gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240 gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgggtagtc 440 <210> 32 <211> 440 <212> DNA <213> OTU433 <400> 32 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aagctctatc agcagggaag acagtgacgg 120 tacctgacta agaagcaccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaagagca agtctgatgt 240 gaaaggctgg ggcttaaccc caggactgca ttggaaactg tttttcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccggtagtc 440 <210> 33 <211> 460 <212> DNA <213> OTU440 <400> 33 cctacgggag gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 cggtgtggca agtctgatgt gaaaggcatg ggctcaacct gtggactgca ttggaaactg 300 tcatacttga gtgccggagg ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360 attaggagga acaccagtgg cgaaggcggc ttactggaca gtaactgacg ttgaggctcg 420 aaagcgtggg gagcaaacag gattagatac ccttgtagtc 460 <210> 34 <211> 442 <212> DNA <213> OTU458 <400> 34 cctacgggcg gctccagtgg ggaatattgc acaatgggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120 ggtacctgac taagaagcat cggctaaata cgtgccagca gccgcggtaa tacgtatgga 180 240 gtgaaaaccc agggcttaac cctgggactg cattggaaac tgtctggctc gagtgccgga 300 gaggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa gaacaccagt 360 ggcgaaggcg gcttactgga cattactga cgttgaggct cgaaaagcgtg gggagcaaac 420 aggattagat acccgagtag tc 442 <210> 35 <211> 460 <212> DNA <213> OTU465 <400> 35 cctacggggg gcagcagtga ggaatattgg tcaatggccg agaggctgaa ccagccaagt 60 cgcgtgaagg aagaaggatc tatggtttgt aaacttcttt tataggggaa taaagtggag 120 gacgtgtcct ttttgtatg tacctatga ataagcatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagttttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420 aaagtgtggg tatcaaacag gattagatac cccagtagtc 460 <210> 36 <211> 442 <212> DNA <213> OTU478 <400> 36 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgaacg aagaagtatt tcggtatgta aagttctatc agcagggaag aagaaatgac 120 ggtacctgac taagaagctc cggctaaata cgtgccagca gccgcggtaa tacgtatgga 180 gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggcggtctta taagtctgat 240 gtgaaagccc ggggctcaac cccgggactg cattggaaac tgtaggacta gagtgtcgga 300 ggggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360 ggcgaaggcg gcttactgga cgattactga cgctgaggct cgaaagcgtg gggagcaaac 420 aggattagat accccagtag tc 442 <210> 37 <211> 440 <212> DNA <213> OTU479 <400> 37 cctacgggtg gctgcagtgg gggatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240 gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctaga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 38 <211> 460 <212> DNA <213> OTU482 <400> 38 cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgaaggtcc tacggattgt aaacttcttt tataagggaa taaaccctcc 120 cacgtgtggg agcttgtatg taccttatga ataagcatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggagcgcaga 240 cgggtcgtta agtcagctgt gaaagtttgg ggctcaacct taaaattgca gttgatactg 300 gcgtccttga gtgcggttga ggtgtgcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc acactaatcc gtaactgacg ttcatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 39 <211> 440 <212> DNA <213> OTU492 <400> 39 cctacgggtg gctgcagtcg agaatcattc acaatggggg aaaccctgat ggtgcgacgc 60 cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120 tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaa cgtagggtgc 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggaccggca agttggaagt 240 gaaatccatg ggctcaaccc gtgaattgct ttcaaaactg ctggccttga gtagtgcaga 300 ggtaggtgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcgac ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420 gattagatac ccttgtagtc 440 <210> 40 <211> 440 <212> DNA <213> OTU493 <400> 40 cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaaaccatg ggctcaaccc ataaactgct ttcaaaactg tttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 41 <211> 440 <212> DNA <213> OTU507 <400> 41 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg atgaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggtgcgtagg cggctttgca agtcagatgt 240 gaaatctatg ggctcaaccc atagcctgca tttgaaactg cagagcttga gtgaagtaga 300 ggcaggcgga attccccgtg tagcggtgaa atgcgtagag atggggagga acaccagtgg 360 cgaaggcggc ctgctgggct ttaactgacg ctgaggcacg aaagcgtggg tagcaaacag 420 gattagatac ccgagtagtc 440 <210> 42 <211> 460 <212> DNA <213> OTU513 <400> 42 cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagtattc 120 cacgtgtggg attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gtttccttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactagact gcaactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 43 <211> 460 <212> DNA <213> OTU517 <400> 43 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg taagcctgaa ccagccaagt 60 cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgcgg 120 gacgtgtccc gttttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300 gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactagact gttactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 44 <211> 460 <212> DNA <213> OTU530 <400> 44 cctacggggg gcagcagtga ggaatattgg tcaatgggcg cgagcctgaa ccagccaagt 60 cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgcgg 120 gacgtgtccc gttttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagttttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460 <210> 45 <211> 465 <212> DNA <213> OTU531 <400> 45 cctacgggag gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60 cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120 agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg tatacggag gatccgagcg ttatccggat ttattgggtt taaagggagc 240 gtagatggat gttaagtca gttgtgaaag tttgcggctc aaccgtaaaa ttgcagttga 300 tactggatat cttgagtgca gttgaggcag gcggaattcg tggtgtagcg gtgaaatgct 360 tagatatcac gaagaactcc gattgcgaag gcagcctgct aagctgcaac tgacattgag 420 gctcgaaagg gtgggtatca aacaggatta gataccccag tagtc 465 <210> 46 <211> 460 <212> DNA <213> OTU536 <400> 46 cctacgggag gcagcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60 cgcgtgcagg atgacggctc tatgagttgt aaactgcttt tgtactaggg taaactcacc 120 tacgtgtagg tgactgaaag tatagtacga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggattc aagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctggact gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460 <210> 47 <211> 460 <212> DNA <213> OTU548 <400> 47 cctacgggtg gctgcagtga ggaatattgg tcaatggacg cgagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagttgtc 120 cacgtgtgga ttttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gcagtcttga gtgcagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggagt gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 48 <211> 440 <212> DNA <213> OTU569 <400> 48 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240 gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 49 <211> 439 <212> DNA <213> OTU587 <400> 49 cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtaggtggc 180 gagcgttgtc cggaattatt gggcgtaaag agcatgtagg cggcttaata agtcgagcgt 240 gaaaatgcgg ggctcaaccc cgtatggcgc tggaaactgt taggcttgag tgcaggagag 300 gaaaggggaa ttcccagtgt agcggtgaaa tgcgtagata ttgggaggaa caccagtggc 360 gaaggcgcct ttctggactg tgtctgacgc tgagatgcga aagccagggt agcgaacggg 420 attagatacc cctgtagtc 439 <210> 50 <211> 440 <212> DNA <213> OTU597 <400> 50 cctacgggcg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgagaca agtctgaagt 240 gaaagcccgg ggctcaaccc cgggactgct ttggaaactg ccttgctaga gtgctggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccctgtagtc 440 <210> 51 <211> 460 <212> DNA <213> OTU606 <400> 51 cctacgggcg gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttact gggtgtaaag ggagcgtaga 240 cggcaaggca agtctgatgt gaaaacccag ggcttaaccc tgggactgca ttggaaactg 300 tctggctgga gtgccggaga ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360 attaggaaga acaccagtgg cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg 420 aaagcgtggg gagcaacag gattagatac cctagtagtc 460 <210> 52 <211> 465 <212> DNA <213> OTU609 <400> 52 cctacgggtg gcagcagtgg ggaattttgg aaatgggcg caagcctgat ccagctattc 60 cgcgtgtggg atgaaggccc tcgggttgta aaccactttt gtagagaacg aaaagacacc 120 atcgataaa tggtgttgct gacggtactc tagagaataag caccggctaa ctacgtgcca 180 gcagccgcgg tatacgtag ggtgcgagcg ttaatcggaa ttactgggcg taaagggtgc 240 gcaggcggtt gagtaagaca gatgtgaaat ccccgagctt aactcgggaa tggcatatgt 300 gactgctcga ctagagtgtg tcagagggag gtggaattcc acgtgtagca gtgaaatgcg 360 tagatattag gaggaacacc agtggcgaag gcggcttact ggacgataac tgacgctgag 420 gctcgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 53 <211> 465 <212> DNA <213> OTU625 <400> 53 cctacgggtg gcagcagtag ggaatctttcg gcaatggacg gaagtctgac cgagcaacgc 60 cgcgtgagtg aagaaggttt tcggatcgta aagctctgtt gtaagagaag aacgagtgtg 120 agagtggaaa gttcacactg tgacggtatc ttaccagaaa gggacggcta actacgtgcc 180 agcagccgcg gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag 240 cgcaggcggt tagataagtc tgaagttaaa ggctgtggct taaccatagt acgctttgga 300 aactgtttaa cttgagtgca agaggggaga gtggaattcc atgtgtagcg gtgaaatgcg 360 tagatatatg gaggaacacc ggtggcgaaa gcggctctct ggcttgtaac tgacgctgag 420 gctcgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 54 <211> 460 <212> DNA <213> OTU634 <400> 54 cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacggggaa taaagtgagg 120 cacgtgtgcc ttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttact gggtgtaaag ggagcgtaga 240 cggatgggca agtctgatgt gaaaacccgg ggctcaaccc cgggactgca ttggaaactg 300 tctggctcga gtgccggaga ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360 attaggaaga acaccagtgg cgaaggcggc ttactggact gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cccggtagtc 460 <210> 55 <211> 440 <212> DNA <213> OTU656 <400> 55 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag agattgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240 gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 56 <211> 440 <212> DNA <213> OTU671 <400> 56 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgatta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240 gaaagactgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcgga ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccggtagtc 440 <210> 57 <211> 440 <212> DNA <213> OTU676 <400> 57 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cggaggatcc 180 gagcgttatc cggatttatt gggtttaaag ggagcgtaga tggatgttta agtcagttgt 240 gaaagtttgc ggctcaaccg taaattgca gttgatactg gatgtcttga gttcagttga 300 ggcaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360 cgaaggcagc ctgctaatct gcgactgaca ttgaggctcg aaagtgtggg tatcaacag 420 gattagatac ccgggtagtc 440 <210> 58 <211> 459 <212> DNA <213> OTU678 <400> 58 cctacgggtg gctgcagtga ggaatattgg tcaatggacg taagtctga ccagccaagt 60 cgcgtgagggg aagactgccc tattggttgt aaacctcttt tataagggaa gaataagttc 120 tacgtgtaga atgatgcctg taccttatga ataagcatcg gctaactccg tgccagcagc 180 cgcggtatata cggaggatgc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240 cggtttatta agttagtggt taaatattg agctaactc aattgtgcca ttaatactgg 300 taaactggag tacagacgag gtaggcggaa taagttaagt agcggtgaa tgcatagata 360 taacttagaa ctccgatagc gaggcagct taccagactg taactgacgc tgatgcacga 420 gagcgtgggt agcgaacagg attagatacc ctagtagtc 459 <210> 59 <211> 440 <212> DNA <213> OTU703 <400> 59 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggagaaca agttggaagt 240 gaaatccatg ggctcaaccc atgaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggcacg aaggccaggg gagcgaaagg 420 gattagatac cccagtagtc 440 <210> 60 <211> 441 <212> DNA <213> OTU707 <400> 60 cctacgggag gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaatgacg 120 gtacctgact aagaagctcc ggctaaatac gtgccagcag ccgcggtaat acgtatggag 180 caagcgttat ccggaattac tgggtgtaaa gggtgcgtag gtggtatggc aagtcagaag 240 tgaaaaccca gggcttaact ctgggactgc ttttgaaact gtggaactgg agtgcaggag 300 aggtaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg aacaccagtg 360 gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420 ggattagata ccctagtagt c 441 <210> 61 <211> 460 <212> DNA <213> OTU717 <400> 61 cctacgggag gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatatgggaa taaagtattc 120 cacgtgtgga atttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300 gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactagact gtcactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac ccctgtagtc 460 <210> 62 <211> 460 <212> DNA <213> OTU724 <400> 62 cctacgggag gctgcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60 cgcgtgcagg aagacggctc tatgagttgt aaactgcttt tgtacgaggg taaacgctct 120 tacgtgtaag agcctgaaag tatcgtacga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggattc aagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240 cggtttgata agttagaggt gaaatttcgg ggctcaaccc tgaacgtgcc tctaatactg 300 ttgagctaga gagtagttgc ggtaggcgga atgtatggtg tagcggtgaa atgcttagag 360 atcatacaga acaccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420 aaagcgtggg gagcaaacag gattagatac cccagtagtc 460 <210> 63 <211> 440 <212> DNA <213> OTU736 <400> 63 cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg tggcaaggca agccagaagt 240 gaaaacccgg ggctcaaccg cgggattgct tttggaactg tcatgctaga gtgcaggagg 300 ggtgagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360 cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 64 <211> 440 <212> DNA <213> OTU752 <400> 64 cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120 tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggctgtgca agtctgaagt 240 gaaaggcatg ggctcaacct gtggactgct ttggaaactg tgcagctaga gtgtcggagaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 65 <211> 442 <212> DNA <213> OTU757 <400> 65 cctacgggtg gctccagtgg ggaatattgc aaatgggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120 ggtacctgac taagaagcac cggctaaata cgtgccagca gccgcggtaa tacgtaggtg 180 gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggacttt taagtgagat 240 gtgaaatacc cgggctcaac ttgggtgctg catttcaaac tggaagtcta gagtgcagga 300 gaggagaatg gaattcctag tgtagcggtg aaatgcgtag agattaggaa gaacaccagt 360 ggcgaaggcg attctctgga ctgtaactga cgctgaggct cgaaagcgtg gggagcaaac 420 aggattagat accccagtag tc 442 <210> 66 <211> 465 <212> DNA <213> OTU763 <400> 66 cctacgggtg gcagcagtag ggaatcttcc acaatgggcg aaagcctgat ggagcaacgc 60 cgcgtgtgtg atgaagggtt tcggctcgta aaacactgtt gtaagagaag aatgacattg 120 180 gcagccgcgg taatacgtat gttccaagcg ttatccggat ttatgggcg taaagcgagc 240 gcagacggtt atttaagtct gaagtgaaag ccctcagctc aactgagaa ttgctttgga 300 aactggatga cttgagtgca gtagagaaa gtggaactcc atgtgtagcg gtgaaatgcg 360 tagatatg gaagaacacc agtggcgaag gcggctttct ggactgtaac tgacgttgag 420 gctcgaaagt gtgggtagca aacaggatta gataccctag tagtc 465 <210> 67 <211> 460 <212> DNA <213> OTU771 <400> 67 cctacgggtg gctgcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60 cgcgtgcagg aagacggctc tatgagttgt aaactgcttt tgtactaggg taaacgcttc 120 tacgtgtagg agcctgaaag tatagtacga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300 gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactagact gcaactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cccagtagtc 460 <210> 68 <211> 440 <212> DNA <213> OTU774 <400> 68 cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgtagg tggcaaggca agccagaagt 240 gaaaacccgg ggctcaaccg cgggattgct tttggaactg tcatgctaga gtgcaggagg 300 ggtgagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360 cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 69 <211> 444 <212> DNA <213> OTU777 <400> 69 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgaagg aagaaggttt tcggatcgta aacttctatc aacagggacg aagaaagtga 120 cggtacctga ataagaagcc ccggctaact acgtgccagc agccgcggta atacgtaggg 180 ggcaagcgtt atccggaatt actgggtgta aagggagcgt aggcggcacg ccaagccaga 240 tgtgaaagcc cgaggcttaa cctcgcggat tgcatttgga actggcgagc tagagtacag 300 gagaggaaag cggaattcct agtgtagcgg tgaaatgcgt agatattagg aagaacacca 360 gtggcgaagg cggctttctg gactgaaact gacgctgagg ctcgaaagcg tggggagcaa 420 acaggattag ataccctagt agtc 444 <210> 70 <211> 439 <212> DNA <213> OTU780 <400> 70 cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcaacgc 60 cgcgtgagcg atgaaggcct tcgggtcgta aagctctgtc ctcaaggaag ataatgacgg 120 tacttgaga ggaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 tagcgttatc cggatttact gggcgtaaag ggtgcgtagg cggtctttta agtcaggagt 240 gaaggctac ggctcaaccg tagtaagctc ttgaaactgg aggacttgag tgcaggagag 300 gagaggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggagaa caccagtagc 360 gaaggcggct ctctggactg taactgacgc tgaggcacga aagcgtgggg agcaaacagg 420 attagatacc ctagtagtc 439 <210> 71 <211> 440 <212> DNA <213> OTU783 <400> 71 cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag acagtgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggtatcaca agtcagaagt 240 gaaagcccgg ggctcaaccc cgggactgct tttgaaactg tggaactgga gtgcaggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 72 <211> 440 <212> DNA <213> OTU787 <400> 72 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtcagatgt 240 gaaaacccgg ggctcaaccc cgggactgca tttgaaactg tcggactaga gtgccggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 73 <211> 460 <212> DNA <213> OTU793 <400> 73 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgaccgccc tatgggttgt aaacttcttt tatatgggaa taaagggtgc 120 cacgtgtggc attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggacatgta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300 cgtgtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cccagtagtc 460 <210> 74 <211> 440 <212> DNA <213> OTU801 <400> 74 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgaggca agtctgatgt 240 gaaaacccgg ggctcaaccc cgtgactgca ttggaaactg tttgcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gcaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 75 <211> 440 <212> DNA <213> OTU828 <400> 75 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaatccatg ggctcaaccc atgaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 76 <211> 440 <212> DNA <213> OTU844 <400> 76 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtc ccaggggacg ataatgacgg 120 taccctggga ggaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtagggtgc 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggattggca agttgggagt 240 gaaatctatg ggctcaaccc ataaattgct ttcaaaactg tcagtcttga gtggtgtaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ctactgggca ctaactgacg ctgaggctcg aaagcatggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 77 <211> 440 <212> DNA <213> OTU845 <400> 77 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg tggcaaggca agccagaagt 240 gaaaacccgg ggctcaaccg cgggattgct tttggaactg tcatgctaga gtgcaggagg 300 ggtgagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360 cgaaggcgat tctctggact gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccctgtagtc 440 <210> 78 <211> 442 <212> DNA <213> OTU846 <400> 78 cctacgggtg gctgcagtga ggaatcttcc acaatgggcg aaagcctgat ggagcaacgc 60 cgcgtgaagg atgaaggcct tcgggttgta aacttcttttt atgagtgaag aatatgacgg 120 taactcatga ataagcaccg gctaactacg tgccagcagc cgcggtcata cgtagggtgc 180 aagcattatc cggagtgact gggcgtaaag agttgcgtag gcggtttaat aagtgaatag 240 tgaaacctgg tggctcaacc atacagacta ttattcaaac tgttaaactc gagaatggta 300 gaggtaactg gaatttctag tgtaggagtg aaatccgtag atattagaag gaacaccgat 360 ggcgtaggca ggttactggg ccatttctga cgctaaggca cgaaagcgtg gggagcgaac 420 cggattagat acccgagtag tc 442 <210> 79 <211> 440 <212> DNA <213> OTU850 <400> 79 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240 gaaaggccgg ggctcaaccc cggagctgca ttgaaactg ctcggctaga gtacaggaga 300 ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 80 <211> 440 <212> DNA <213> OTU866 <400> 80 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggaaggcta agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 81 <211> 460 <212> DNA <213> OTU870 <400> 81 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg taagcctgaa ccagccaagt 60 cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgtgg 120 gacgtgtcct attttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgagga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 82 <211> 440 <212> DNA <213> OTU873 <400> 82 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaagtgacgg 120 tacctgacta agaagccccg gctaattacg tgccagcagc cgcggtaata cgtaaggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtttggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcagacttga gtgccggaga 300 ggcaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctgctggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctggtagtc 440 <210> 83 <211> 440 <212> DNA <213> OTU875 <400> 83 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg atgaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactccg tgccagcagc cgcggtaata cggaggatcc 180 gagcgttatc cggatttatt gggtttaaag ggagcgtagg tggattgtta agtcagttgt 240 gaaagtttgc ggctcaaccg taaaattgca gttgaaactg gcagtcttga gtacagtaga 300 ggtggggcgga attcgtggtg tagcggtgaa atgcttggat atcacgaaga actccgattg 360 cgaaggcagc tcactagact gcaactgaca ctgatgctcg aaagtgtggg tatcaaacag 420 gattagatac ccgagtagtc 440 <210> 84 <211> 440 <212> DNA <213> OTU893 <400> 84 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcagcgca agtctgaagt 240 gaaagcccgg ggctcaaccc cggaatggct ttggaaactg tgcagctaga gtaccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 85 <211> 460 <212> DNA <213> OTU944 <400> 85 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggactggta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 tcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420 aaagtgtggg tatcaaacag gattagatac ccttgtagtc 460 <210> 86 <211> 460 <212> DNA <213> OTU948 <400> 86 cctacgggag gcagcagtga ggaatattgg tcaatgggcg taagcctgaa ccagccaagt 60 cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgtgg 120 gacgtgtcct attttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240 cggcctttta agtcagcggt gaaagtctgt ggctcaacca tagaattgcc gttgaaactg 300 ggaggcttga gtatgtttga ggcaggcgga atgcgtggtg tagcggtgaa atgcttagat 360 atcacgcaga accccgattg cgaaggcagc ctgccaagcc atgactgacg ctgatgcacg 420 aaagcgtggg gatcaaacag gattagatac ccctgtagtc 460 <210> 87 <211> 442 <212> DNA <213> OTU949 <400> 87 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120 ggtacctgac taagaagcac cggctaaata cgtgccagca gccgcggtaa tacgtatggt 180 gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggtgtgg caagtctgat 240 gtgaaaggca tgggctcaac ctgtggactg cattggaaac tgtcatactt gagtgccgga 300 ggggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360 ggcgaaggcg gcttactgga cgataactga cgctgaggct cgaaagcgtg gggagcaaac 420 aggattagat accctagtag tc 442 <210> 88 <211> 465 <212> DNA <213> OTU962 <400> 88 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60 cgcgtgtgtg aagaaggcct tcgggttgta aagcactttc agcggggagg aaggcggtga 120 ggttaataac ctcatcgatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtc tgtcaagtcg gatgtgaat ccccgggctc aacctgggaa ctgcattcga 300 aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 89 <211> 440 <212> DNA <213> OTU983 <400> 89 cctacgggag gcagcagtgg ggaatattgc acatggggg aaccctgat gcagcgacgc 60 cgcgtggagg agaaggtct tcggattgta aactcctgtt gttgaggaag atatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttatc cggatttact gggtgtaag ggagcgtaga cggcgacgca agtctgaagt 240 gaaatacccg gggctcaacct gggaactgct tggaactg tgttgctga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtga atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 90 <211> 460 <212> DNA <213> OTU1002 <400> 90 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg atggcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttact gggtgtaaag ggagcgcaga 240 cggcactgca agtctgaagt gaaagcccgg ggctcaaccc cgggactgct ttggaaactg 300 tagagctaga gtgctggaga ggcaagcgga attcctagtg tagcggtgaa atgcgtagat 360 attaggaaga acaccagtgg cgaaggcggc ttactggaca gtaactgacg ttgaggctcg 420 aaagcgtggg gagcaaacag gattagatac cctagtagtc 460 <210> 91 <211> 440 <212> DNA <213> OTU1013 <400> 91 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120 tacctgagga ggaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 180 gagcgttgtc cggatttact gggcgtaaag ggagcgtagg cggactttta agtgagatgt 240 gaaatacccg ggctcaactt gggtgctgca tttcaaactg gaagtctaga gtgcaggaga 300 ggagaatgga attcctagtg tagcggtgaa atgcgtagag attaggaaga acaccagtgg 360 cgaaggcgat tctctggact gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 92 <211> 440 <212> DNA <213> OTU1024 <400> 92 cctacgggcg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240 gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctaga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 93 <211> 440 <212> DNA <213> OTU1038 <400> 93 cctacggggg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240 gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggagg 360 cgaaggcggc tcactggact gtactgaca ctgaggctcg aaagcgtggg gagcaacag 420 ccgagtagtc 440 <210> 94 <211> 465 <212> DNA <213> OTU1053 <400> 94 cctacgggag gcagcagtgg ggaatattgc acatggggcg caagcctgat gcagccatgc 60 cgcgtgtag aagaaggcct tcggttgta aagtactttc agcggggagg aagggagtaa 120 agttaatacc ttgctcatt gacgttacc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtc tgtcaagtcg gatgtgaat ccccgggctc aacctgggaa ctgcattcga 300 aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaagacc ggtggcgaag gcggccccct ggacgagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gatacccgtg tagtc 465 <210> 95 <211> 442 <212> DNA <213> OTU1064 <400> 95 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60 cgcgtgaagg aagaaggctt tcgggttgta aacttcttttt gtcagggacg agcagaagac 120 ggtacctgac gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180 gcaagcgttg tccggattta ctgggtgtaa agggcgtgta gccgggtcgg caagtcagat 240 gtgaaatcca cgggcttaac tcgtgaactg catttgaaac tgttgatctt gagtatcgga 300 gaggcaatcg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360 ggcgaaggcg gattgctgga cgacaactga cggtgaggcg cgaaagcgtg gggagcaaac 420 aggattagat accccagtag tc 442 <210> 96 <211> 440 <212> DNA <213> OTU1070 <400> 96 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgtgtc cggaattact gggtgtaaag ggagcgtaga cggcgaagca agtctgaagt 240 gaaaacccag ggctcaaccc tgggactgct ttggaaactg ttttgctaga gtgtcggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 97 <211> 440 <212> DNA <213> OTU1081 <400> 97 cctacgggag gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggacgggca agtctgatgt 240 gaaagcccgg ggcttaaccc cgggactgca ttggaaactg tccatcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccctgtagtc 440 <210> 98 <211> 440 <212> DNA <213> OTU1150 <400> 98 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaagagca agtctgatgt 240 gaaaggctgg ggcttaaccc caggactgca ttggaaactg ttttcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 99 <211> 440 <212> DNA <213> OTU1157 <400> 99 cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120 tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaa cgtagggtgc 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaaaccatg ggctcaaccc atgaattgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtagatgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggt ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 100 <211> 440 <212> DNA <213> OTU1163 <400> 100 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagt aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgatgca agtctgaagt 240 gaaatacccg ggctcaacct gggaactgct ttggaaactg tatggctaga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 101 <211> 460 <212> DNA <213> OTU1170 <400> 101 cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagc 120 cacgtgtggc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cgggttgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gcgaccttga gtgcaacaga ggtaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ttactggatt gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 102 <211> 460 <212> DNA <213> OTU1188 <400> 102 cctacgggag gcagcagtga ggaatattgg tcaatgggcg caggcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatgtatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240 cggtttgata agttagaggt gaaatcccgg ggcttaactc cggaactgcc tctaatactg 300 ttagactaga gagtagttgc ggtaggcgga atgtatggtg tagcggtgaa atgcttagag 360 atcatacaga acaccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420 aaagcgtggg gagcaaacag gattagatac cccagtagtc 460 <210> 103 <211> 440 <212> DNA <213> OTU1205 <400> 103 cctacgggag gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagcaacgc 60 cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120 tacctgagga ggaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 180 gagcgttgtc cggatttact gggcgtaaag ggagcgtagg cggattttta agtgagatgt 240 gaaatactcg ggcttaacct gagtgctgca tttcaaactg gaagtctaga gtgcaggaga 300 ggagaaggga attcctagtg tagcggtgaa atgcgtagag attaggaaga acaccagtgg 360 cgaaggcgct tctctggact gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccctgtagtc 440 <210> 104 <211> 445 <212> DNA <213> OTU1207 <400> 104 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg caagcctgat gcagcgacgc 60 cgcgtgaggg atggaggcct tcgggttgta aacctctttt atcggggagc aagcgagagt 120 gagtttaccc gttgaataag caccggctaa ctacgtgcca gcagccgcgg taatacgtag 180 ggtgcaagcg ttatccggaa ttattgggcg taaagggctc gtaggcggtt cgtcgcgtcc 240 ggtgtgaaag tccatcgctt aacggtggat ccgcgccggg tacgggcggg cttgagtgcg 300 gtaggggaga ctggaattcc cggtgtaacg gtggaatgtg tagatatcgg gaagaacacc 360 aatggcgaag gcaggtctct gggccgttac tgacgctgag gagcgaaagc gtggggagcg 420 aacaggatta gataccctag tagtc 445 <210> 105 <211> 440 <212> DNA <213> OTU1208 <400> 105 cctacgggtg gcagcagtag ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatt tcggtatgta aacttctatc agcaaggaag aaaatgacgg 120 tacttgacta agaagccccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggaaggcta agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg atgactgacg ttgaggcttg aaagcgtggg gagcaaacag 420 gattagatac cctggtagtc 440 <210> 106 <211> 443 <212> DNA <213> OTU1271 <400> 106 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgaagg aagaagtatt tcggtatgta aacttctatc gacagggaag aaacaaatga 120 cggtacctga ataagaagca ccggctaaat acgtgccagc agccgcggta atacgtatgg 180 tgcaagcgtt atccggattt actgggtgta aagggtgagt aggcggtcat gcaagtcata 240 tgtgaaatgt cagggcttaa cttggcgct gcataagaaa ctgtatgact agagtgcagg 300 agaggtaagc ggaattccta gtgtagcggt gaaatgcgta gatattagga agaacaccgg 360 tggcgaaggc ggcttactgg actgttactg acgctgagtc acgaaagcgt ggggagcaaa 420 caggattaga taccctagta gtc 443 <210> 107 <211> 440 <212> DNA <213> OTU1274 <400> 107 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cacggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggctgtgta agtctgaagt 240 gaaagcccgg ggctcaaccc cgggactgct ttggaaacta tgcagctaga gtgtcggaga 300 ggtaagtgga attcccagtg tagcggtgaa atgcgtagat attgggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 108 <211> 460 <212> DNA <213> OTU1276 <400> 108 cctacgggcg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacggggaa taaagtgagg 120 cacgtgtgcc ttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtatata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240 cggcctttta agtcagcggt gaagtctgt ggctcaacca tegattgcc gttgaactg 300 gggggcttga gtatgtttga ggcaggcgga atgcgtggtg tagcggtga atgcttagat 360 atcacgcaga accccgattg cgaaggcagc ctgccaagcc atgactgacg ctgatgcacg 420 aaagcgtggg gatcaacag gattagatc cctggtagtc 460 <210> 109 <211> 465 <212> DNA <213> OTU1298 <400> 109 cctacgggtg gctgcagtag ggaatcttcg gcaatggggg caaccctgac cgagcaacgc 60 cgcgtgagtg aagaaggttt tcggatcgta aagctctgtt gtaagagaag aacgtgtgtg 120 agagtggaaa gttcacacag tgacggtaac ttaccagaaa gggacggcta actacgtgcc 180 agcagccgcg gtaatacgta ggtcccgagc gttgtccgga tttattgggc gtaaagcgag 240 cgcaggcggt ttaatagtc tgaagttaaa ggcagtggct taaccattgt tcgctttgga 300 aactgttaga cttgagtgca gaaggggaga gtggaattcc atgtgtagcg gtgaatgcg 360 tagatatag gaggaacacc ggtggcgaaa gcggctctct gtctgtaac tgacgctgag 420 gctcgaaagc gtggggagca aacaggatta gataccccag tagtc 465 <210> 110 <211> 442 <212> DNA <213> OTU1359 <400> 110 cctacgggtg gcagcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60 cgcgtgaagg aagaggctt tcggttgta aacttcttt aagtgggaag agtagagac 120 ggtaccactt gaataagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 180 gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccgggcatg caagtcagat 240 gtgaaatctc agggcttaac cctgaactg catttgaac tgtagtctt gagtgccgga 300 gaggtaatcg gattccttg tgtagcggtg aaatgcgtag ataggaa gaacaccagt 360 ggcgaaggcg gattactgga cggtaactga cggtgaggcg cgaagcgtg gggagcgaac 420 aggtag acctagtag tc 442 <210> 111 <211> 440 <212> DNA <213> OTU1377 <400> 111 cctacgggag gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggcacagca agtcagaagt 240 gaaagcccgg ggctcaaccc cgggactgct tttgaaactg ttgtgctgga gtgcaggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagcgg 360 cgaaggcggc ttactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgggtagtc 440 <210> 112 <211> 460 <212> DNA <213> OTU1404 <400> 112 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatatgggaa taaagttttc 120 cacgtgtgga atttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagttttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactagact gttactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 113 <211> 441 <212> DNA <213> OTU1412 <400> 113 cctacgggtg gctgcagtgg ggaatctttgc gcaatggggg gaaccctgac gcagcgacgc 60 cgcgtgcggg acggaggcct tcgggcgta aaccgctttc agcagggaag agtcaagact 120 gtacctgcag aagaagcccc ggctaactac gtgccagcag ccgcggtaat acgtaggggg 180 cgagcgttat ccggattcat tgggcgtaaa gcgcgcgtag gcggcccggc aggccggggg 240 tcgaagcggg gggctcaacc cccgaagcc cccggaacct cccggcttg ggtccggtag 300 gggagggtgg aacacccggt gtagcggtgg aatgcgcaga tatcgggtgg aacaccggtg 360 gcgaaggcgg ccctctgggc cgagaccgac gctgaggcgc gaaagctggg ggagcgaaca 420 ggattagata ccctagtagt c 441 <210> 114 <211> 440 <212> DNA <213> OTU1413 <400> 114 cctacgggcg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120 tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaatctatg ggctcaaccc ataaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 115 <211> 441 <212> DNA <213> OTU1420 <400> 115 cctacgggtg gctgcagtgg gggatattgc gcaatggggg caaccctgac gcagcaacgc 60 cgcgtgaagg atgaaggttt tcggattgta aacttcttttt attaaggacg aaaaatgacg 120 gtacttaatg aataagctcc ggctaactac gtgccagcag ccgcggtaat acgtaggggg 180 caagcgttat ccggatttac tgggtgtaaa gggagcgtag acggtgtggc aagtctgatg 240 tgaaaggcat gggctcaacc tgtggactgc attggaaact gtcatacttg agtgccggag 300 gggtaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg aacaccagtg 360 gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420 ggattagata ccccagtagt c 441 <210> 116 <211> 440 <212> DNA <213> OTU1444 <400> 116 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg atgaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggttatgta agtctgatgt 240 gaaaacccgg ggctcaaccc cgggactgca ttggaaacta tgtaactaga gtgtcggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg atgactgacg gtgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 117 <211> 440 <212> DNA <213> OTU1446 <400> 117 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240 gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggt ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 118 <211> 440 <212> DNA <213> OTU1450 <400> 118 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtacggca agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcggactaga gtgtcggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 119 <211> 465 <212> DNA <213> OTU1464 <400> 119 cctacgggtg gctgcagtag ggaatcttcc acaatggacg caagtctgat ggagcaacgc 60 cgcgtgagtg aagaaggttt tcggatcgta aagctctgtt gttggtgaag aaggatagag 120 gtagtaactg gcctttattt gacggtaatc aaccagaaag tcacggctaa ctacgtgcca 180 gcagccgcgg tatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc 240 gcaggcggaa gaataagtct gatgtgaaag ccctcggctt aaccgaggaa ctgcatcgga 300 aactgttttt cttgagtgca gaagaggaga gtggaactcc atgtgtagcg gtggaatgcg 360 tagatatatg gaagaacacc agtggcgaag gcggctctct ggtctgcaac tgacgctgag 420 gctcgaaagc atgggtagcg aacaggatta gataccctag tagtc 465 <210> 120 <211> 440 <212> DNA <213> OTU1467 <400> 120 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240 gaaagcccgg ggctcaaccc cgggactgca ttggaaactg tcgtacttga gtatcggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac ccttgtagtc 440 <210> 121 <211> 440 <212> DNA <213> OTU1476 <400> 121 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg atgaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggcatggca agtcagaagt 240 gaaagcctgg ggctcaaccc cggaattgct tttgaaactg tcaggctaga gtgtcggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtactgacg ttgaggctcg aaagcgtggg gagcaacag 420 gattagatc ccctgtagtc 440 <210> 122 <211> 465 <212> DNA <213> OTU1488 <400> 122 cctacgggtg gctgcagtgg ggaatattgc acatggggcg caagcctgat gcagccatgc 60 cgcgtgtgtg aagaaggcct tcggttgta aagcactttc agcggggagg aaggcgttaa 120 ggttaataac cttggcgatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcgagcg ttaatcggaa taactggggcg taaagggcac 240 gcaggcggtg acttaagtga ggtgtgaaag ccccggctc aacctgggaa ctgcatctga 300 tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gatacccgtg tagtc 465 <210> 123 <211> 440 <212> DNA <213> OTU1502 <400> 123 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg atgaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggagtggca agtctgatgt 240 gaaaacccgg ggctcaaccc cgggactgca ttggaaactg tcaatctaga gtaccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 124 <211> 449 <212> DNA <213> OTU1503 <400> 124 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgcggg atggaggcct tcgggttgta aaccgctttt gatcgggagc aagcccttcg 120 gggtgagtgt acctttcgaa taagcaccgg ctaactacgt gccagcagcc gcggtaatac 180 gtagggtgca agcgttatcc ggaattattg ggcgtaaagg gctcgtaggc ggttcgtcgc 240 gtccggtgtg aaagcccatc gcttaacggt gggtctgcgc cgggtacggg cgggctggag 300 tgcggtaggg gagactggaa ttcccggtgt aacggtggaa tgtgtagata tcgggaagaa 360 caccaatggc gaaggcaggt ctctgggccg tcactgacgc tgaggagcga aagcgtgggg 420 agcgaacagg attagatacc cttgtagtc 449 <210> 125 <211> 440 <212> DNA <213> OTU1518 <400> 125 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag ataatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggcggagca agtcagaagt 240 gaaagcccgg ggctcaaccc cgggacggct tttgaaactg ccctgcttga tttcaggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggact gacaatgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 126 <211> 440 <212> DNA <213> OTU1522 <400> 126 cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240 gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 127 <211> 459 <212> DNA <213> OTU1538 <400> 127 cctacggggg ctgcagtgag gaatattggt caatgggcgc aggcctgaac cagccaagta 60 gcgtgaagga tgactgccct atgggttgta aacttctttt ataaaggaat aaagtcgggt 120 atgtataccc gtttgcatgt actttatgaa taaggatcgg ctaactacgt gccagcagcc 180 gcggtaatac gtagggggca agcgttatcc ggatttactg ggtgtaaagg gagcgtagac 240 ggtgtggcaa gtctgatgtg aaaggcatgg gctcaacctg tggactgcat tggaaactgt 300 catacttgag tgccggaggg gtaagcggaa ttcctagtgt agcggtgaaa tgcgtagata 360 ttaggaagaa caccagtggc gaaggcggct tactggacag taactgacgt tgaggctcga 420 aagcgtgggg agcaaacagg attagatacc ccagtagtc 459 <210> 128 <211> 440 <212> DNA <213> OTU1556 <400> 128 cctacgggag gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240 gaaaggccgg ggctcaaccc cggagctgca tttgaaactg ctcggctaga gtacaggaga 300 ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 129 <211> 460 <212> DNA <213> OTU1563 <400> 129 cctacgggtg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120 cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggggtatta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gtatccttga gtgcagcaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggagt gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460 <210> 130 <211> 440 <212> DNA <213> OTU1564 <400> 130 cctacgggag gctgcagtgg gggatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag acagtgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggact gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 131 <211> 440 <212> DNA <213> OTU1599 <400> 131 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcaggaaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggttttgca agtctgaagt 240 gaaagcccgg ggcttaaccc cgggactgct ttggaaactg taggactaga gtgcaggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggact gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 132 <211> 460 <212> DNA <213> OTU1605 <400> 132 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatggatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cgtatggtgc aagcgttatc cggatttact gggtgtaaag ggagcgcagg 240 cggtgcggca agtctgatgt gaaagcccgg ggctcaaccc cggtactgca ttggaaactg 300 tcgtactaga gtgtcggagg ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360 attaggagga acaccagtgg cgaaggcggc ttactggacg gtaactgacg ttgaggctcg 420 aaagcgtggg gagcaaacag gattagatac ccgagtagtc 460 <210> 133 <211> 440 <212> DNA <213> OTU1612 <400> 133 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cggaggatgc 180 gagcgttatc cggatttatt gggtttaaag ggagcgtaga cggatggaca agtctgatgt 240 gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccggtgg 360 cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccggtagtc 440 <210> 134 <211> 460 <212> DNA <213> OTU1626 <400> 134 cctacgggag gcagcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgcct tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cgtagggggc aagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 135 <211> 440 <212> DNA <213> OTU1637 <400> 135 cctacgggcg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggaagagca agtctgatgt 240 gaaaggctgg ggcttaaccc caggactgca ttggaaactg ttttcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgggtagtc 440 <210> 136 <211> 440 <212> DNA <213> OTU1641 <400> 136 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcaggaaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggcatcaca agtcagaagt 240 gaaagcccgg ggctcaaccc cgggactgct tttgaaactg tggagctgga gtgcaggaga 300 ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 137 <211> 460 <212> DNA <213> OTU1661 <400> 137 cctacgggtg gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120 cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggagt gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460 <210> 138 <211> 460 <212> DNA <213> OTU1675 <400> 138 cctacgggtg gctgcagtga ggaatattgg tcaatggacg caagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagtattc 120 cacgtgtggg attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 139 <211> 440 <212> DNA <213> OTU1680 <400> 139 cctacgggag gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtcaagca agtcagaagt 240 gaaaggctgg ggctcaaccc cgggactgct tttgaaactg ttgactgga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 140 <211> 440 <212> DNA <213> OTU1683 <400> 140 cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120 tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaa cgtagggtgc 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggaccggca agttggaagt 240 gaaaactatg ggctcaaccc ataaattgct ttcaaaactg ctggccttga gtagtgcaga 300 ggtaggtgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcgac ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420 gattagatac cccagtagtc 440 <210> 141 <211> 440 <212> DNA <213> OTU1713 <400> 141 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggctttgca agtctgacgt 240 gaaactccgg ggctcaactc cggaactgcg ttggaaactg taaggcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gcaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 142 <211> 440 <212> DNA <213> OTU1716 <400> 142 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggaattact gggtgtaaag ggagcgtaga cggtaaagca agtctgaagt 240 gaaagcccgg ggctcaaccc cgggactgct ttggaaactg tttaactaga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 143 <211> 440 <212> DNA <213> OTU1742 <400> 143 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 144 <211> 440 <212> DNA <213> OTU1758 <400> 144 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggactggca agtctgatgt 240 gaaaggcggg ggctcaaccc ctggactgca ttggaaactg tgtctttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccctgtagtc 440 <210> 145 <211> 440 <212> DNA <213> OTU1774 <400> 145 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactccg tgccagcagc cgcggtaata cggaggatcc 180 gagcgttatc cggatttatt gggtttaaag ggagcgtaga tgggttgtta agtcagttgt 240 gaaagtttgc ggctcaaccg taaaattgca attgatactg gcagccttga gtacagttga 300 ggtaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360 cgaaggcagc tcactggact gcaactgaca ctgatgctcg aaagtgtggg tatcaaacag 420 gattagatac cccagtagtc 440 <210> 146 <211> 440 <212> DNA <213> OTU1789 <400> 146 cctacgggtg gcagcagtag ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag acaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggacgggca agtctgaagt 240 gaaaggcagg ggctcaaccc ctggactgct ttggaaactg tccatctaga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgggtagtc 440 <210> 147 <211> 440 <212> DNA <213> OTU1805 <400> 147 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaatctatg ggcttaaccc ataaactgct ttcaaaactg ctggtcttga gtgatggaga 300 ggcaggcgga attccgtgtg tagcggtgaa atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac ccgagtagtc 440 <210> 148 <211> 443 <212> DNA <213> OTU1813 <400> 148 cctacgggtg gctgcagtgg ggaatattgc gcaatggggg caaccctgac gcagcaacgc 60 cgcgtgcagg aagaaggtct tcggattgta aactgttgtc gcaagggaag aagacagtga 120 cggtaccttg tgagaaagtc acggctaact acgtgccagc agccgcggta atacgtaggt 180 gandaagcgtt gtccggattt actgggtgta aagggcgcgt agggcggactg tcaagtcagt 240 cgtgaaatac cggggcttaa ccccggggct gcgattgaaa ctgacagcct tgagtatcgg 300 agaggaaagc ggaattccta gtgtagcggt gaatgcgta gatattagga ggacaccag 360 tggcgaaggc ggctttctgg acgacaactg acgctgaggc gcgaaagtgt ggggagcaaa 420 Tacctagtagtc 443 <210> 149 <211> 440 <212> DNA <213> OTU1824 <400> 149 cctacggtg gctgcagtgg ggaatattgc acatggagg aactctgat gcagcgacgc 60 cgcgtgagtg aagagtaat tcgttatgta aagctctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaag ggagtgtagg tggcatcaca agtcagaagt 240 gaaagcccgg gggctcaaccc cgggactgct ttgaactg tggagctgga gtgcaggaga 300 ggcaagtgga attcctagtg tagcggtga atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 150 <211> 440 <212> DNA <213> OTU1830 <400> 150 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatgggca agtctgatgt 240 gaaaacccgg ggctcaaccc cgggactgca ttggaaactg ttcatctaga gtgctggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc cccctggaca aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 151 <211> 442 <212> DNA <213> OTU1831 <400> 151 cctacgggtg gctgcagtgg ggaatattgg gcaatggacg caagtctgac ccagcaacgc cgcgtgaagg aagaaggctt tcggggttgta aacttctttt gtcagggaag agtagaagac 180. ggtacctgac gaatagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccggggccgg caagtcagat 240 gtgaatctg last ctccaaactg catttgaac tgtaggtctt last gaggttatcg gattccttg tgtagcggtg aaatgcgtag atatagga gaacaccagt ggcgaaggcg gataactgga cggcaactga cggtgaggcg cgaaagcgtg gggagcaaac acquire accctagtag tc 442 <210> 152 <211> 465 <212> DNA <213> OTU1835 <400> 152 cctacgggag gcagcagtag ggaatcttcg gcaatggggg caaccctgac cgagcaacgc cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120 agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtt tgttaagtca gatgtgaat ccccgggctc aacctgggaa ctgcatctga 300 tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 153 <211> 443 <212> DNA <213> OTU1841 <400> 153 cctacgggtg gctgcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc 60 cgcgtgaagg agaaggctt tcggttgta aacttcttt atgagggacg aaggaagtga 120 cggtacctca tgaataagct ccggctaact acgtgccagc agccgcggta atacgtaggg 180 agcgagcgtt atccggattt actgggtgta aagggcgtgt aggcgggaa gcaagtcaga 240 tgtgaaaacc agtggctcaa ccactggcct gcatttgaaa ctgtttttct tgagtgatgg 300 agaggcaggc ggaattccgt gtgtagcggt gaatgcgta gatatacgga ggaacaccag 360 tggcgaaggc ggcctgctgg acattaactg acgctgaggc gcgaaagcgt ggggagcaaa 420 caggattaga taccctagta gtc 443 <210> 154 <211> 440 <212> DNA <213> OTU1844 <400> 154 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtaaagca agtctgaagt 240 gaaagcccgg ggctcaaccc cgggactgct ttggaaactg tttaactaga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 155 <211> 440 <212> DNA <213> OTU1848 <400> 155 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240 gaaaggccgg ggctcaaccc cggagctgca ttgaaactg ctcggctaga gtacaggaga 300 ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 156 <211> 440 <212> DNA <213> OTU1858 <400> 156 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga tggatgttta agtcagttgt 240 gaaagtttgc ggctcaaccg taaaattgca gttgatactg gatatcttga gtgcagttga 300 ggcaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360 cgaaggcagc acactaatcc gtaactgacg ttcatgctcg aaagtgtggg tatcaaacag 420 gattagatac cccagtagtc 440 <210> 157 <211> 441 <212> DNA <213> OTU1859 <400> 157 cctacgggcg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaatgacg 120 gtacctgact aagaagcacc ggctaaatac gtgccagcag ccgcggtaaa acgtagggtg 180 caagcgttgt ccggaattac tgggtgtaaa gggagcgtag gtggcaaggc aagccagaag 240 tgaaaacccg gggctcaacc gcgggattgc ttttggaact gtcatgctag agtgcaggag 300 gggtgagcgg aattcctagt gtagcggtga aatgcgtaga tattaggaag aacaccagtg 360 gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420 ggattagata cccgggtagt c 441 <210> 158 <211> 440 <212> DNA <213> OTU1880 <400> 158 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagtgtagg tggccaggca agtcagaagt 240 gaaagcccgg ggctcaaccc cgggactgct tttgaaactg cagggctaga gtgcaggagg 300 ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 159 <211> 465 <212> DNA <213> OTU1909 <400> 159 cctacgggtg gctgcagtgg ggaatattgc acatggggcg caagcctgat gcagccatgc 60 cgcgtgtag aagaaggcct tcggttgta aagtactttc agcggggagg aagggagtaa 120 agttaatacc ttgctcatt gacgttacc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtt tgttaagtca gatgtgaat ccccgggctc aacctgggaa ctgcatctga 300 tactggcaag cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 160 <211> 460 <212> DNA <213> OTU1918 <400> 160 cctacgggtg gctgcagtga ggaatattgg tcaatggacg agatctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacggga taaagtgagg 120 cacgtgtgcc ttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ttgctggact gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 161 <211> 440 <212> DNA <213> OTU1919 <400> 161 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggaaggcta agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg gtcatctaga gtgtcggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctggtagtc 440 <210> 162 <211> 440 <212> DNA <213> OTU1936 <400> 162 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaa cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 163 <211> 465 <212> DNA <213> OTU1951 <400> 163 cctacgggtg gctccagtgg ggaatcttcc gcaatggacg aaagtctgac ggagcaacgc 60 cgcgtgagtg atgacggcct tcgggttgta aagctctgtg atcggggacg aatggctggt 120 atgctaatac catatcagag tgacggtacc cgaatcaa gccacggcta actacgtgcc 180 agcagccgcg gtaatacgta ggtggcaagc gttgtccgga attattgggc gtaaagcgcg 240 cgcaggcggc ttcttaagtc catcttaaaa gtgcggggct taaccccgtg atgggatgga 300 aactgggagg ctggagtatc ggagagaaa gtggaattcc tagtgtagcg gtgaaatgcg 360 tagagattag gaaacacc ggtggcgaag gcgactttct ggacgacaac tgacgctgag 420 gcgcgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 164 <211> 440 <212> DNA <213> OTU1972 <400> 164 cctacgggtg gctccagtgg ggaatattgc aaatgggg aaaccctgat gcagcgacgc 60 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggtgc 180 aagcgttaat cggaattact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 165 <211> 440 <212> DNA <213> OTU1977 <400> 165 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaatccatg ggctcaaccc atgaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 166 <211> 440 <212> DNA <213> OTU1979 <400> 166 cctacgggtg gctgcagtgg ggaatttttgc gcaatggggg aaaccctgac gcagcaacgc 60 cgcgtgcggg acgacggcct tcgggttgta aaccgctttc agcagggaag aaattcgacg 120 gtacctgcag aagaagctcc ggctaactac gtgccagcag ccgcggtaat acgtagggag 180 cgagcgttat ccggattcat tgggcgtaaa gagcgcgtag gcggcctc aagcgggatc 240 tctaatccga gggctcaacc cccggccgga tcccgaactg ggaggctcga gttcggtaga 300 ggcaggcgga attcccggtg tagcggtgga atgcgcagat atcgggaaga acaccgatgg 360 cgaaggcagc ctgctgggcc gcaactgacg ctgaggcgcg aaagctaggg gagcgaacag 420 gattagatac cctagtagtc 440 <210> 167 <211> 460 <212> DNA <213> OTU2006 <400> 167 cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagtattc 120 cacgtgtggg attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300 gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420 aaagcgtggg gagcaaacag gattagatac ccctgtagtc 460 <210> 168 <211> 465 <212> DNA <213> OTU2048 <400> 168 cctacggggg gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc 60 cgcgtgtatg aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa 120 agttaatacc tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtt gattgagtca gatgtgaat cccggggctt aacccggga ttgcatctga 300 tactggtcag ctagagtctt gtagaggggg gtagaatttcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacgagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gatacccctg tagtc 465 <210> 169 <211> 440 <212> DNA <213> OTU2052 <400> 169 cctacggcg gcagcagtgg ggaatattgc acatggggg aaccctgat gcagcgacgc 60 cgcgtgagcg aagagtatt tcggtagta aacttctatc agcagggaag aaatgacgg 120 tacctgacta agaagccccg gctactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaag ggagcgtagg tggcagtgca agtcagatgt 240 gaaaggccgg ggctcaaccc cggagctgca ttgaactg ctcggctga gtacaggaga 300 ggcaggcgga attcctagtg tagcggtga atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctgctggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 170 <211> 440 <212> DNA <213> OTU2086 <400> 170 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcagcgca agtctgaagt 240 gaaagcccgg ggctcaaccc cggaatggct ttggaaactg tgcagctaga gtaccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 171 <211> 440 <212> DNA <213> OTU2096 <400> 171 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagcaccg gctaaatacg tgccagcagc cgcggtaata cgtatggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 172 <211> 440 <212> DNA <213> OTU2112 <400> 172 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggtatcaca agtcagaagt 240 gaaagcccgg ggctcaaccc cgggactgct tttgaaactg tggaactgga gtgcaggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 173 <211> 440 <212> DNA <213> OTU2162 <400> 173 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240 gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 174 <211> 440 <212> DNA <213> OTU2198 <400> 174 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggcagtgca agtcagatgt 240 gaaaggccgg ggctcaaccc cggagctgca ttgaaactg ctcggctaga gtacaggaga 300 ggcaggcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ctgctggact gttactgaca ctgaggcacg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 175 <211> 472 <212> DNA <213> OTU2219 <400> 175 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaaccc cgggactgca tttggaactg tcaggctaga gtgtcggaga 300 ggaaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc tttctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc catctgtctc tggattagat accctagtag tc 472 <210> 176 <211> 439 <212> DNA <213> OTU2221 <400> 176 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgatgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggaattatt gggcgtaaag agggagcagg cggcactaag ggtctgtggt 240 gaaagatcga agcttaactt cggtaagcca tggaaaccgt agagctagag tgtgtgagag 300 gatcgtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc 360 gaaggcgacg atctggcgca taactgacgc tgaggctcga aagcgtgggg agcaaacagg 420 attagatacc cgagtagtc 439 <210> 177 <211> 440 <212> DNA <213> OTU2285 <400> 177 cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtaat tcgttatgta aagctctatc agcagggaag atagtgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggccatgca agtcagaagt 240 gaaaatccgg ggctcaaccc cggaactgct tttgaaactg tgaggctgga gtgcaggagg 300 ggtgagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 178 <211> 440 <212> DNA <213> OTU2294 <400> 178 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggacgctta agtcagttgt 240 gaaagtttgc ggctcaaccg taaaattgca gttgatactg ggtgtcttga gtacagtaga 300 ggcaggcgga attcgtggtg tagcggtgaa atgcttagat atcacgaaga actccgattg 360 cgaaggcagc ctgctggact gtaactgacg ctgatgctcg aaagtgtggg tatcaaacag 420 gattagatac cctagtagtc 440 <210> 179 <211> 439 <212> DNA <213> OTU2306 <400> 179 cctacgggtg gctgcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcaacgc 60 cgcgtgagcg atgaaggcct tcgggcgta aagctctgtc ctcaaggaag ataatgacgg 120 tacttgagga ggaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 tagcgttatc cggaattact gggcgtaaag ggtgcgtagg tggtttctta agtcagaggt 240 gaaaggctac ggctcaaccg tagtaagcct ttgaaactgg gaaacttgag tgcaggagag 300 gagagtggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaggaa caccagttgc 360 gaaggcggct ctctggactg taactgacac tgaggcacga aagcgtgggg agcaaacagg 420 attagatacc ctagtagtc 439 <210> 180 <211> 440 <212> DNA <213> OTU2312 <400> 180 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag atagtgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180 aagcgttatc cggatttatt gggtgtaaag ggtgcgcaga cggcgatgca agtctggagt 240 gaaagcccgg ggctcaaccc cgggactgct ttggaaactg tatggctaga gtgctggaga ggcaagcgga attcctagtg tagcggtga atgcgtagt attack acaccagtgg cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420 440. ccggggtagtc <210> 181 <211> 442 <212> DNA <213> OTU2325 <400> 181 cctacgggtg gctgcagtgg ggaatattgg gcaatgggcg caagcctgac ccagcaacgc cgcgtgaagg aagaaggctt tcggggttgta aacttctttt gtcagggacg agtagaagac 120 180. ggtacctgac gaatagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta gccggggaggg caagtcagat 240 gtgaaatcca cgggctcaac tcgtgaactg catttgaaac tactcttctt gagtatcgga gaggcaatcg gattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt ggcgaaggcg gattgctgga cgacaactga cggtgaggcg cgaaagcgtg gggagcaaac 420 aggattagat accctagtag tc 442 <210> 182 <211> 440 <212> DNA <213> OTU2334 <400> 182 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg atgaagtatt tcggtatgta aacttctatc ggcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtatggca agtctgatgt 240 gaaaggccag ggctcaaccc tgggactgca ttggaaactg tcgaactaga gtgtcggaga 300 ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 183 <211> 443 <212> DNA <213> OTU2385 <400> 183 cctacgggtg gctgcagtgg ggaatattgg gcaatggggg aaaccctgac ccagcaacgc 60 cgcgtgaagg aagaaggctt tcgggttgta aacttctttt accagggacg aaggacgtga 120 cggtacctgg agaaaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt 180 ggcaagcgtt gtccggattt actgggtgta aagggcgtgt aggcggagaa gcaagtcaga 240 agtgaaatcc atgggcttaa cccatgaact gcttttgaaa ctgtttccct tgagtatcgg 300 agaggcaggc ggaattccta gtgtagcggt gaaatgcgta gatattagga ggaacaccag 360 tggcgaaggc ggcctgctgg acgacaactg acgctgaggc gcgaaagcgt ggggagcaaa 420 caggattaga taccctagta gtc 443 <210> 184 <211> 440 <212> DNA <213> OTU2397 <400> 184 cctacgggtg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt ttggtatgta aagctctatc agcagggaag acagtgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240 gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 185 <211> 440 <212> DNA <213> OTU2402 <400> 185 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120 tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggactggca agtctgatgt 240 gaaaggcggg ggctcaaccc ctggactgca ttggaaactg tgtcttga gtgtcggagg 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccctgtagtc 440 <210> 186 <211> 465 <212> DNA <213> OTU2407 <400> 186 cctacgggtg gctccagtgg ggaattttgg aaatgggcg caagcctgat ccagctattc 60 cgcgtgtggg atgaaggccc tcgggttgta aaccactttt gtagagaacg aaaagacatc 120 ttcgataaa ggatgttgct gacggtactc tagagaataag caccggctaa ctacgtgcca 180 gcagccgcgg taatacgtag ggtgcgagcg ttaatcggaa ttactgggcg taaagggtgc 240 gcaggcggtt ggagaagaca gatgtgaaat ccccgagctt aactcgggaa tggcatatgt 300 gactgctcga ctagagtgtg tcagagggag gtggaattcc acgtgtagca gtgaaatgcg 360 tagatatgtg gaagaacacc gatggcgaag gcagcctcct gggacataac tgacgctcag 420 gcacgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 187 <211> 460 <212> DNA <213> OTU2409 <400> 187 cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacggga taaagttagc 120 cacgtgtggt ttttgcatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtatata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggggtatta agtcagttgt gaagtttgc ggctcaccg taaaattgca gttgatactg 300 gtatccttga gtgcagcaga ggtgggcgga attcgtggtg tagcggtga atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggagt gtaacgacg ctgatgctcg 420 aaagtgtggg tatcaacag gattagatac cctagtagtc 460 <210> 188 <211> 446 <212> DNA <213> OTU2423 <400> 188 cctacggtg gctgcagtcg agaatcattc acatggggg aaccctgat ggtgcgacgc 60 cgcgtggggg aatgaaggtc ttcggattgt aaacccctgt catgtgggag caattaaaa 120 agatagtacc acaagaggaa gagacggcta actctgtgcc agcagccgcg gtaatacaga 180 ggtctcaagc gttgttcgga atcactgggc gtaaagcgtg cgtaggctt ttcgtaagtc 240 gtgtgtgaaa ggcgcggggct caacccgcgg acggcacatg attackgcgag actagagtaa 300 tggagggga accggaatttc tcggtgtagc agtgaatgc gtagatatcg agaggaacac 360 tcgtggcgaa ggcggttcc tggacattaa ctgacgctga ggcacgaagg ccagggaggc 420 gaaagggatt agatacccta gtagtc 446 <210> 189 <211> 440 <212> DNA <213> OTU2443 <400> 189 cctacggtg gctgcagtgg ggaatattgc acatggcg aaagcctgat gcagcaacgc 60 cgcgtgagtg aagaagtatc tcggtagta aagctctatc agcagggaag aaatgacgg 120 tacctgacta agaagccccg gctactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaag ggagcgcaga cggcactgca agtctgaagt 240 gaaagcccgg gggctcaaccc cgggactgct tggaactg tagagctga gtgctggaga 300 ggcaagcgga attcctagtg tagcggtga atgcgtagat attaggaga acaccagtgg 360 cgaaggcggc ttgctggaca gtaactgacg ttcaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 190 <211> 440 <212> DNA <213> OTU2445 <400> 190 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag ataatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttgaggcttg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 191 <211> 465 <212> DNA <213> OTU2460 <400> 191 cctacgggtg gcagcagtgg ggaatattgc acatggggcg caagcctgat gcagccatgc 60 cgcgtgtag aagaaggcct tcggttgta aagtactttc agcgaggagg aaggtgttgt 120 ggttaataac cgcagcaatt gacgttactc gcagagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtc tgtcaagtcg gatgtgaat ccccgggctc aacctgggaa ctgcatccga 300 aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gatacccctg tagtc 465 <210> 192 <211> 465 <212> DNA <213> OTU2471 <400> 192 cctacgggtg gctgcagtgg ggaatattgc acatggggcg caagcctgat gcagccatgc 60 cgcgtgtatg aagaaggcct tcggttgta aagtactttc agcgaggagg aaggcgttgt 120 ggttaataac cgcaacgatt gacgttactc gcagagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcgga ttactgggcg taaagcgcac gcaggcggtc tgtcaagtcg gatgtgaat ccccgggctc aacctggga ctgcatccga aactggcagg ctagagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 420. tagagatctg gaggaatacc ggtggcgaag gcagccatct ggcctaatac tgacgctgag gtacgaaagc atggggagca aacaggatta gataccccag tagtc <210> 193 <211> 460 <212> DNA <213> OTU2473 <400> 193 cctacggggg gctgcagtga ggaatattgg tcaatgggcg caggcctga ccagccaagt agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaagga taaagtcggg 180. tatgtatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtga atgcttagat atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaacgacg ctgaggctcg 420 aaagtgtggg tagcaac gattagatac ccgagtagtc 460 <210> 194 <211> 465 <212> DNA <213> OTU2499 <400> 194 cctacgggtg gcagcagtgg ggaatattgc acatggggcg caagcctgat gcagccatgc 60 cgcgtgtag aagaaggcct tcggttgta aagtactttc agcgaggagg aaggcattaa 120 ggttaataac cttagtgatt gacgttactc gcagaagaag caccggctaa ctccgtgcca 180 gcagccgcgg taatacggag ggtgcaagcg ttaatcggaa ttactggggcg taaagcgcac 240 gcaggcggtc tgttaagtca gatgtgaat cccggggctc aacctgggaa ctgcatttga 300 aactggcagg cttgagtctt gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg 360 tagagatctg gaggaatacc ggtggcgaag gcggccccct ggacaagac tgacgctcag 420 gtgcgaaagc gtggggagca aacaggatta gataccccag tagtc 465 <210> 195 <211> 440 <212> DNA <213> OTU2514 <400> 195 cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtagt tcgctatgta aagctctatc agcagggaag atagtgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggccaggca agtcagaagt 240 gaaagcccgg ggctcaaccc cgggactgct tttgaaactg cagggctaga gtgcaggagg 300 ggcaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 196 <211> 439 <212> DNA <213> OTU2531 <400> 196 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggtgcgtagg tggtttctta agtcagaggt 240 gaaggctac ggctcaaccg tagtaagcct ttgaaactgg gaaacttgag tgcaggagag 300 gagaggaa ttcctagtgt agcggtgaaa tgcgtagata ttacgaggaa caccagttgc 360 gaaggcggct ctctggacag taactgacgt tcaggctcga aagcgtgggg agcaaacagg 420 attagatacc ctagtagtc 439 <210> 197 <211> 439 <212> DNA <213> OTU2533 <400> 197 cctacggtgg cagcagtggg gaatattgca caatgggcga aagcctgatg cagcaacgcc 60 120 acctgactaa gaagccccgg ctaactacgt gccagcagcc gcggtaatac gtagggggca 180 agcgttatcc ggatttactg ggtgtaaagg gagcgtagac ggcgcagcaa gtctgatgtg 240 aaaggcaggg gcttaacccc tggactgcat tggaaactgc tgtgcttgag tgccggaggg 300 gtaagcggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaggaa caccagtggc 360 gaaggcggct tgctggacag taactgacgt tcaggctcga aagcgtgggg agcaaacagg 420 attagatacc ccggtagtc 439 <210> 198 <211> 443 <212> DNA <213> OTU2541 <400> 198 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgaagg atgacggttt tcggattgta aacttcttttt cttagtgacg aagacagtga 120 cggtagctaa ggaataagca tcggctaact acgtgccagc agccgcggta atacgtagga 180 tgcaagcgtt atccggattt actgggtgta aagggagcgc aggcggggact gcaagttgga 240 tgtgaaatac cgtggcttaa ccacggaact gcatccaaaa ctgtagttct tgagtgaagt 300 agaggcaagc ggaattccga gtgtagcggt gaaatgcgta gatattcgga ggaacaccag 360 tggcgaaggc ggcttgctgg gctttaactg acgctgaggc tcgaaagtgt ggggagcaaa 420 caggattaga taccctagta gtc 443 <210> 199 <211> 460 <212> DNA <213> OTU2564 <400> 199 cctacgggtg gcagcagtga ggaatattgg tcaatgggcg caggcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatgtatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggacgctta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 ggtgtcttga gtacagtaga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420 aaagtgtggg tatcaaacag gattagatac ccgtgtagtc 460 <210> 200 <211> 439 <212> DNA <213> OTU2568 <400> 200 cctaggtggc tgcagtgggg aatattgcac aatggaggaa actctgatgc agcgacgccg 60 cgtgaaggat gaagtatttc ggtatgtaaa gctctatcag cagggaagaa gaatgacggt 120 acctgactaa gaagcaccgg ctaactacgt gccagcagcc gcggtaatac gtagggggca 180 agcgttatcc ggatttactg ggtgtaaagg gagcgtagac ggtgtggcaa gtctgatgtg 240 aaaggcatgg gctcaacctg tggactgcat tggaaactgt catacttgag tgccggaggg 300 gtaagcggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaagaa caccagtggc 360 gaaggcggct tactggacgg taactgacgt tgaggctcga aagcgtgggg agcaaacagg 420 attagatacc cttgtagtc 439 <210> 201 <211> 440 <212> DNA <213> OTU2600 <400> 201 cctacgggtg gcagcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120 tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaatctatg ggctcaaccc ataaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcgac ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 202 <211> 465 <212> DNA <213> OTU2601 <400> 202 cctacgggtg gcagcagtag ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc 60 cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg 120 agagtaactg ttcatccctt gacggtatct aaccagaaag ccacggctaa ctacgtgcca 180 gcagccgcgg tatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc 240 gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga 300 aactgggaga cttgagtgca gaagaggaga gtggaattcc atgtgtagcg gtgaaatgcg 360 tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgag 420 gctcgaaagc gtggggagca aacaggatta gataccctag tagtc 465 <210> 203 <211> 440 <212> DNA <213> OTU2618 <400> 203 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120 tacctgagga ggaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 180 aagcgttgtc cggatttact gggcgtaaag ggagcgtagg cggactttta agtgagatgt 240 gaaatacccg ggctcaactt gggtgctgca tttcaaactg gaagtctaga gtgcaggaga 300 ggagaatgga attcctagtg tagcggtgaa atgcgtagag attaggaaga ataccagtgg 360 cgaaggcgcc tttctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccggtagtc 440 <210> 204 <211> 440 <212> DNA <213> OTU2626 <400> 204 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtaat tcgttatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggccgtgca agtctgatgt 240 gaaaggctgg ggctcaaccc cgggactgca ttggaaactg tatggctgga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctggtagtc 440 <210> 205 <211> 440 <212> DNA <213> OTU2637 <400> 205 cctacgggtg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtagt tcgctatgta aagctctatc agcagggaag ataatgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaatccatg ggctcaaccc atgaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 206 <211> 460 <212> DNA <213> OTU2647 <400> 206 cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacgggaa taaagtgagg 120 cacgtgtgcc tttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtgtaaag ggagcgcagg 240 cggtacggca agtctgatgt gaaagcccgg ggctcaaccc cggtactgca ttggaaactg 300 tcggactaga gtgtcggagg ggtaagtgga attcctagtg tagcggtgaa atgcgtagat 360 attaggagga acaccagtgg cgaaggcggc ttactggacg gtaactgacg ttgaggctcg 420 aaagcgtggg gagcaaacag gattagatac cccagtagtc 460 <210> 207 <211> 440 <212> DNA <213> OTU2686 <400> 207 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgagta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggt caactggacg ataactgacg ctgaggcgcg aaagcgtggg gagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 208 <211> 460 <212> DNA <213> OTU2693 <400> 208 cctacgggag gcagcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacggggaa taaagtgagc 120 cacgtgtggc ttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagttttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ttactggatt gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 209 <211> 440 <212> DNA <213> OTU2695 <400> 209 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcaggaaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagtgcagg cgggaagaca agttggaagt 240 gaaatccatg ggctcaaccc atgaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ttactggact gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 210 <211> 440 <212> DNA <213> OTU2703 <400> 210 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggatggaca agtctgatgt 240 gaaaggctgg ggctcaaccc cgggactgca ttggaaactg cccgtcttga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 211 <211> 440 <212> DNA <213> OTU2717 <400> 211 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatc tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcggagca agtctgaagt 240 gaaagcccgg ggctcaaccc cgggactgct ttggaaactg ttctgctaga gtgctggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccggtagtc 440 <210> 212 <211> 440 <212> DNA <213> OTU2721 <400> 212 cctacgggtg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag acagtgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcaga cggcactgca agtctgaagt 240 gaaagcccgg ggctcaaccc cgggactgca ttggaaactg tcaatctaga gtaccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 213 <211> 442 <212> DNA <213> OTU2730 <400> 213 cctacgggtg gctccagtgg ggaatattgc aaatgggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaaatgac 120 ggtacctgac taagaagcac cggctaaata cgtgccagca gccgcggtaa tacgtatggt 180 gcaagcgtta tccggattta ctgggtgtaa agggagcgca ggcggaaggc taagtctgat 240 gtgaaagccc ggggctcaac cccggtactg cattgggaaac tggtcatcta gagtgtcgga 300 ggggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag gaacaccagt 360 ggcgaaggcg gcttactgga cgataactga cgctgaggct cgaaagcgtg gggagcaaac 420 aggattagat accctagtag tc 442 <210> 214 <211> 460 <212> DNA <213> OTU2734 <400> 214 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg caggcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatatgggaa taaagttttc 120 cacgtgtgga attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggacagtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gctgtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactggact gcaactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 215 <211> 440 <212> DNA <213> OTU2735 <400> 215 cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgtggg aagaaggttt tcggattgta aaccactgtt cttggtgaag ataatgacgg 120 taaccaagga gaaagctccg gctaactacg tgccagcagc cgcggtaata cgtagggagc 180 aagcgttgtc cggatttact gggtgtaaag ggtgcgtagg cggctaggca agtcagacgt 240 gaaaaccatg ggctcaacct gtggactgcg ttgaaactg tttagcttga gtgaagtaga 300 ggtaggcgga attcccggtg tagcggtgaa atgcgtagag atcgggagga acaccagtgg 360 cgaaggcggc ctactgggct ttaactgacg ctgaggcacg aaagcatggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 216 <211> 440 <212> DNA <213> OTU2736 <400> 216 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtagg cggtctgaca agtcagaagt 240 gaaagcccgg ggctcaactc cgggactgct ttgaaactg ccggactaga ttgcaggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggact gtaaatgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 217 <211> 440 <212> DNA <213> OTU2770 <400> 217 cctacggggg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240 gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctgga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc tcactggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctggtagtc 440 <210> 218 <211> 439 <212> DNA <213> OTU2813 <400> 218 cctacgggtg gcagcagtgg gggatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcgac ttactggact gctactgaca ctgaggcacg aaagcgtggg gagcaaacag 420 gattagatac ccagtagtc 439 <210> 219 <211> 440 <212> DNA <213> OTU2819 <400> 219 cctacgggtg gctgcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcgacgca agtctgaagt 240 gaaatacccg ggctcaacct gggaactgct ttggaaactg tgttgctaga gtgctggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 220 <211> 440 <212> DNA <213> OTU2835 <400> 220 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgaagg aagaagtatc tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgcagg cgggaagaca agttggaagt 240 gaaatctatg ggctcaaccc ataaactgct ttcaaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg tagcaaacag 420 gattagatac ccctgtagtc 440 <210> 221 <211> 440 <212> DNA <213> OTU2851_dkd43_100000030018 <400> 221 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcatgaca agccagatgt 240 gaaaacccag ggctcaaccc tgggactgca tttggaactg ccaggctgga gtgcaggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg atgactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccttgtagtc 440 <210> 222 <211> 440 <212> DNA <213> OTU2867 <400> 222 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaagtgacgg 120 tacctgaata agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggcaaggca agtctgaagt 240 gaaagcccgg tgcttaacgc cgggactgct ttggaaactg ttggctgga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 223 <211> 440 <212> DNA <213> OTU2879_hc56_100000053763 <400> 223 cctacgggtg gctgcagtgg ggaatattgg acaatgggcg caagcctgat ccagcaatgc 60 cgcgtgagtg atgaaggccc tagggttgta aagctctttt acccgggaag ataatgactg 120 taccgggaga ataagccccg gctaactccg tgccagcagc cgcggtaata cggagggggc 180 tagcgttgtt cggaattact gggcgtaaag cgcacgtagg cggctttgta agtcagaggt 240 gaaagcctgg agctcaactc cagaactgcc tttgagactg catcgcttga atccaggaga 300 ggtcagtgga attccgagtg tagaggtgaa attcgtagat attcggaaga acaccagtgg 360 cgaaggcggc tgactggact ggtattgacg ctgaggtgcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 224 <211> 440 <212> DNA <213> OTU2918 <400> 224 cctacgggtg gcagcagtgg ggaatattgg gcaatggacg caagtctgac ccagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaag ggagcgcagg cgggagaca agttggaagt 240 gaaatctatg ggctcaaccc ataactgct ttcaaactg ttttcttga gtagtgcaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggc ctgctggact gtactgacg ctgatgctcg aaagtgtggg tatcaacag 420 gattagatac ccttgtagtc 440 <210> 225 <211> 465 <212> DNA <213> OTU2933 <400> 225 cctacggtg gctgcagtgg ggaatctcc gcaatggacg aaagtctgac ggagcaacgc 60 cgcgtgagtg atgaaggatt tcggtctgta aagctctgtt gtttatgacg aacgtgcagt 120 gtgtgaacaa tgcattgcaa tgacggtagt aaacgaggaa gccacggcta actacgtgcc 180 agcagccgcg gtaatacgta ggtggcgagc gttgtccgga attattgggc gtaagagca 240 tgtaggcggc ttaataagtc gagcgtgaa atgcggggct caacccgta tggcgctgga 300 aactgttagg cttgagtgca ggagaggaaa gggaattcc cagtgtagcg gtgaatgcg 360 tagatattgg gaggaacacc agtggcgaag gcgccttttct ggactgtgtc tgacgctgag 420 atgcgaaagc cagggtagcg aacgggatta gataccctag tagtc 465 <210> 226 <211> 443 <212> DNA <213> OTU2935 <400> 226 cctacgggtg gctgcagtgg ggaatattgc gcaatggggg aaaccctgac gcagcaacgc 60 cgcgtgattg aagaaggcct tcgggttgta aagatcttta atcagggacg aaacaaatga 120 cggtacctga agaataagct ccggctaact acgtgccagc agccgcggta atacgtaggg 180 agcaagcgtt atccggattt actgggtgta aagggcgcgc aggcgggccg gcaagttgga 240 agtgaaatct atgggcttaa cccataaact gctttcaaaa ctgctggtct tgagtgatgg 300 agaggcaggc ggaattccgt gtgtagcggt gaaatgcgta gatatacgga ggaacaccag 360 tggcgaaggc ggcctgctgg acattaactg acgctgaggc gcgaaagcgt ggggagcaaa 420 caggattaga taccctagta gtc 443 <210> 227 <211> 439 <212> DNA <213> OTU2950 <400> 227 cctagggtgg cagcagtggg gatattgca caatggggga aaccctgatg cagcgacgcc 60 gcgtggagga agaaggtctt cggattgtaa actcctgttg tgaggaga taatgacggt 120 actcacaag gaagtgacgg ctaacgt gccagcagcc gcggtaatac ggaggatccg 180 agcgttatcc ggatttattg gtttaaagg gagcgtaggc ggacgcttaa gtcagttgtg 240 aaagtttgcg gctcaccgt aaattgcag ttgatactgg gtgtctcgg tacagtag 300 gcaggcggaa ttcgtggtgt agcggtgaaa tgcttagata tcacgaagaa ctccgattgc 360 gaaggcagct tgctggactg taactgacgc tgatgctcga aagtgtggct atcaacagg 420 attagatacc cgtgtagtc 439 <210> 228 <211> 460 <212> DNA <213> OTU2954 <400> 228 cctacgggtg gctgcagtga ggaatattgg tcaatggacg agatctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacggga taaagtgagg 120 cacgtgtgcc ttttgtatg taccgtatga ataaggatcg gctaaccg tgccagcagc 180 cgcggtaata cgtagggggc aagcgttatc cggatttact gggtgtaaag ggagcgtaga 240 cggcaaggca agtctgatgt gaaaacccag ggcttaaccc tgggactgca ttggaaactg 300 tctggctcga gtgccggaga ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360 attaggagga acaccagtgg cgaaggcgac taactggact gtaactgacg ctgaggcgcg 420 aaagtgtggg gagcaaacag gattagatac cccagtagtc 460 <210> 229 <211> 440 <212> DNA <213> OTU2955 <400> 229 cctacgggtg gcagcagtgg ggaatattgc acaatgggcg aaagcctgat gcagcgacgc 60 cgcgtgagtg aagaagtatt tcggtatgta aagctctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggtaaagca agtctgaagt 240 gaaagcccgc ggctcaactg cgggactgct ttggaaactg tttaactgga gtgtcggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 230 <211> 440 <212> DNA <213> OTU2997 <400> 230 cctacgggcg gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgaagg atgaagtatt tcggtatgta aacttctatc agcagggaag aaaatgacgg 120 tacctgacta agaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggatttact gggtgtaaag ggagcgtaga cggagcagca agtctgatgt 240 gaaaacccgg ggctcaaccc cgggactgca ttggaaactg ttgatctgga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 231 <211> 440 <212> DNA <213> OTU3044 <400> 231 cctacgggag gcagcagtgg ggaatattgc acaatggagg aaactctgat gcagcgacgc 60 cgcgtgagtg aagaagtagt tcgctatgta aagctctatc agcagggaag atagtgacgg 120 tacctgacta agaagctccg gctaaatacg tgccagcagc cgcggtaata cgtatggagc 180 aagcgttatc cggatttact gggtgtaaag ggagtgtagg tggccaggca agtcagaagt 240 gaaaacccag ggcttaactc tgggactgct ttgaaactg tcagactgga gtgcaggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttgctggact gtaactgaca ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cctagtagtc 440 <210> 232 <211> 466 <212> DNA <213> OTU3055 <400> 232 cctacgggtg gctgcagtgg ggaatattgc gcaatgggcg aaagcctgac gcagcgacgc 60 cgcgtgaggg atgaaggttc tcggatcgta aacctctgtc aggggggaag aaaccccctc 120 gtgtgaataa tgcgagggct tgacggtacc cccaaaggaa gcaccggcta actccgtgcc 180 agcagccgcg gtaatacgga gggtgcaagc gttaatcgga atcactgggc gtaaagcgca 240 cgtaggcggc ttggtaagtc aggggtgaaa tcccacagcc caactgtgga actgccttg 300 atactgccag gcttgagtac cggagagggt ggcggaattc caggtgtagg agtgaaatcc 360 gtagatatct ggaggaacac cggtggcgaa ggcggccacc tggacggtaa ctgacgctga 420 ggtgcgaaag cgtgggtagc aaacaggatt agatacccta gtagtc 466 <210> 233 <211> 440 <212> DNA <213> OTU3061 <400> 233 cctacgggtg gctgcagtgg gggatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggttt tcggattgta aactcctgtc gttagggacg ataatgacgg 120 tacctaacaa gaaagcaccg gctaactacg tgccagcagc cgcggtaaa cgtagggtgc 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggattggca agttgggagt 240 gaaatctatg ggctcaaccc ataaattgct ttcaaaactg tcagtcttga gtggtgtaga 300 ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga acaccagtgg 360 cgaaggcggt ctactgggca ccaactgacg ctgaggctcg aaagcatggg tagcaaacag 420 gattagatac cctagtagtc 440 <210> 234 <211> 440 <212> DNA <213> OTU3065 <400> 234 cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120 tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgtgtc cggaattact gggtgtaaag ggagcgcagg cggtgcggca agtctgatgt 240 gaaagcccgg ggctcaaccc cggtactgca ttggaaactg tcgtactaga gtgtcggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac cccagtagtc 440 <210> 235 <211> 439 <212> DNA <213> OTU3087 <400> 235 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgaagg atgaaggcct ttgggtcgta aacttctgtt ctaagggaag atagtgacgg 120 taccttagga gcaagtcccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 aagcgttatc cggaattatt gggcgtaaag agtacgtagg tggttttcta agcacggggt 240 ttaaggcaat ggcttaacca ttgttcgcct tgtgaactgg aagacttgag tgcaggagag 300 gaaagcggaa ttcctagtgt agcggtgaaa tgcgtagata ttaggaggaa caccagtggc 360 gaaggcggct ttctggactg taactgacac tgaggtacga aagcgtgggg agcaaacagg 420 attagatacc ctagtagtc 439 <210> 236 <211> 440 <212> DNA <213> OTU3094 <400> 236 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttggggaag ataatgacgg 120 tacccaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cggtctggca agtctgatgt 240 gaaaatccgg ggctcaactc cggaactgca ttggaaactg tcagactaga gtgtcggaga 300 ggtaagtgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggacg ataactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgtgtagtc 440 <210> 237 <211> 460 <212> DNA <213> OTU3102 <400> 237 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 tggatgttta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gatatcttga gtgcagttga ggcaggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ctgctaagct gcaactgaca ttgaggctcg 420 aaagtgtggg tatcaaacag gattagatac cctagtagtc 460 <210> 238 <211> 460 <212> DNA <213> OTU3111 <400> 238 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgaaggctc tatgggtcgt aaacttcttt tatatgggaa taaagttttc 120 cacgtgtgga attttgtatg taccatatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 cggggtatta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gtatccttga gtgcagcata ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactagact gtcactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460 <210> 239 <211> 440 <212> DNA <213> OTU3112 <400> 239 cctacgggtg gcagcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtggagg aagaaggtct tcggtttgta aactcctgtt gttgaggaag ataatgacgg 120 tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa cgtaggtcac 180 aagcgttgtc cggaattact gggtgtaaag ggagcgtaga cggcaaggca agtctgatgt 240 gaaaacccag ggcttaaccc tgggactgca ttggaaactg tctggctcga gtgccggaga 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggaaga acaccagtgg 360 cgaaggcggc ttactggacg attactgacg ctgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccgagtagtc 440 <210> 240 <211> 460 <212> DNA <213> OTU3128 <400> 240 cctacgggtg gcagcagtga ggaatattgg tcaatggacg caagtctgaa ccagccatgc 60 cgcgtgcagg aagacggctc tatgagttgt aaactgcttt tgtacgagag taaacgctct 120 tacgtgtaag agcctgaaag tatcgtacga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc aagcgttatc cggatttatt gggtttaaag ggtgcgtagg 240 cggtttgata agttagaggt gaaataccgg tgcttaacac cggaactgcc tctaatactg 300 ttgaactaga gagtagttgc ggtaggcgga atgtatggtg tagcggtgaa atgcttagag 360 atcatacaga acaccgattg cgaaggcagc ttaccaaact atatctgacg ttgaggcacg 420 aaagcgtggg gagcaaacag gattagatac cctagtagtc 460 <210> 241 <211> 465 <212> DNA <213> OTU3131 <400> 241 cctacgggtg gctgcagtag ggaatcttcc acaatggacg caagtctgat ggagcaacgc 60 cgcgtgagtg aagaagggtt tcggctcgta aagctctgtt gttggtgaag aaggacaggg 120 gtagtaactg acctttgttt gacggtaatc aattagaaag tcacggctaa ctacgtgcca 180 gcagccgcgg tatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagt 240 gcaggcggct cgataagtct gatgtgaaag cttcggctc aaccggagaa ttgcatcaga 300 aactgtcgag cttgagtaca gaagaggaga gtggaactcc atgtgtagcg gtgaaatgcg 360 tagatatatg gaagaacacc ggtggcgaag gcggctctct ggtctgttac tgacgctgag 420 gctcgaaagc atgggtagcg aacaggatta gataccctag tagtc 465 <210> 242 <211> 460 <212> DNA <213> OTU3137 <400> 242 cctacgggtg gctgcagtga ggaatattgg tcaatgggcg agagcctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tataaaggaa taaagtcggg 120 tatgcatacc cgtttgcatg tactttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtaga 240 cggtgtggca agtctgatgt gaaaggcatg ggctcaacct gtggactgca ttggaaactg 300 tcatacttga gtgccggagg ggtaagcgga attcctagtg tagcggtgaa atgcgtagat 360 attaggagga acaccagtgg cgaaggcggc tttctggacg acaactgacg ctgaggcgcg 420 aaagtgtggg gagcaaacag gattagatac ccgagtagtc 460 <210> 243 <211> 441 <212> DNA <213> OTU3143 <400> 243 cctacgggag gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcgacgc 60 cgcgtgagcg aagaagtatt tcggtatgta aagctctatc agcagggaag aagaatgacg 120 gtacctgact aagaagcacc ggctaactac gtgccagcag ccgcggtaaa acgtaggtca 180 caagcgttgt ccggaattac tgggtgtaaa gggagcgcag gcgggagaac aagttggaag 240 tgaaatccat gggctcaacc catgaactgc tttcaaaact gttttccttg agtagtgcag 300 aggtaggcgg aattcccggt gtagcggtgg aatgcgtaga tatcgggagg aacaccagtg 360 gcgaaggcgg cttactggac ggtaactgac gttgaggctc gaaagcgtgg ggagcaaaca 420 ggattagata ccctagtagt c 441 <210> 244 <211> 460 <212> DNA <213> OTU3155 <400> 244 cctacgggtg gctgcagtga ggaatattgg tcaatggacg agagtctgaa ccagccaagt 60 agcgtgaagg atgactgccc tatgggttgt aaacttcttt tatacggggaa taaagttatc 120 cacgtgtgga ttttgtatg taccgtatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggacagtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgatactg 300 gctgtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc ttactggatt gtaactgacg ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac cccagtagtc 460 <210> 245 <211> 440 <212> DNA <213> OTU3157 <400> 245 cctacgggtg gctgcagtgg ggaatattgc acaatggggg aaaccctgat gcagcaacgc 60 cgcgtgagtg atgacggcct tcgggttgta aagctctgtc ttcagggacg ataatgacgg 120 tacttgagga ggaagccccg gctaactacg tgccagcagc cgcggtaata cgtagggggc 180 tagcgttatc cggaattact gggcgtaaag ggtgcgtaga cggtgtggca agtctgatgt 240 gaaaggcatg ggctcaacct gtggactgca ttggaaactg tcatacttga gtgccggagg 300 ggtaagcgga attcctagtg tagcggtgaa atgcgtagat attaggagga acaccagtgg 360 cgaaggcggc ttactggaca gtaactgacg ttgaggctcg aaagcgtggg gagcaaacag 420 gattagatac ccctgtagtc 440 <210> 246 <211> 460 <212> DNA <213> OTU3166 <400> 246 cctacgggtg gcagcagtga ggaatattgg tcaatggccg agaggctgaa ccagccaagt 60 cgcgtgaggg atgaaggttc tatggatcgt aaacctcttt tataagggaa taaagtgtgg 120 gacgtgtcct attttgtatg taccttatga ataaggatcg gctaactccg tgccagcagc 180 cgcggtaata cggaggatcc gagcgttatc cggatttatt gggtttaaag ggagcgtagg 240 tggattgtta agtcagttgt gaaagtttgc ggctcaaccg taaaattgca gttgaaactg 300 gcagtcttga gtacagtaga ggtgggcgga attcgtggtg tagcggtgaa atgcttagat 360 atcacgaaga actccgattg cgaaggcagc tcactagact gtcactgaca ctgatgctcg 420 aaagtgtggg tatcaaacag gattagatac ccgagtagtc 460

Claims

1. Intestinal microbial gene marker combination for the identification of type 2 diabetes, characterized in that, the intestinal microbial gene marker combination is an OTU marker combination, and the OTU marker combination is composed of nucleotide sequence structures with the serial numbers shown in the following table:

2. Use of the intestinal microbial gene marker combination according to claim 1 for the preparation of a differential diagnostic reagent for diabetic patients and healthy people or a kit for the identification and diagnosis of diabetes.

3. The use according to claim 2, characterized in that, the diabetes includes type 2 diabetes.

4. The use according to claim 2, characterized in that, the differential diagnostic reagent for diabetic patients and healthy people or the kit for the identification and diagnosis of diabetes includes sequencing primers for the intestinal microbial gene marker combination, and the sequencing primers include 341F_805R, with an insertion length of 465 bpq and a sequencing length of 2 * 300 bp. The specific structure is: Forward primer: 341F 5’-CCTACGGGNGGCWGCAG-3’; Reverse primer: 805R 5’-GACTACHVGGGTATCTAATCC-3’.

Images