A portable liquid nitrogen fumigation program cryogenic freezer

Abstract

The present invention belongs to the technical field of animal semen cryopreservation, and discloses a portable liquid nitrogen fumigation programmed freezer, which includes a freezing box. The bottom of the freezing box is connected with an equipment box and a storage box. The equipment box and the storage box are connected side by side on the base. The top of the equipment box and the storage box is provided with a first buckle to fix the freezing box on the top of the equipment box and the storage box. The bottom of the base is provided with walking wheels, and the top of the freezing box is hinged with a top cover through a hinge shaft; by setting an independent freezing box and an equipment box, and arranging the controller box outside the freezing box, the freezing chamber is isolated from the electronic control system and the mechanical transmission system, which is convenient for reducing the technical difficulty of equipment manufacturing, prolonging the service life of the equipment, facilitating packaging and transportation, facilitating assembly and combination when needed, facilitating transportation to different animal resource farms, implementing on-site freezing of straw semen, and obtaining a high temperature of about 4 °C by controlling the opening degree of the top cover to avoid difficulties in obtaining high temperature caused entirely by the increase in the distance from the liquid nitrogen surface and other dilemmas.

Description

Technical Field

[0001] The present invention relates to a portable liquid nitrogen fumigation programmed freezer, belonging to the technical field of animal semen freezing technology. Background Art

[0002] Research has proven that cryopreserving semen is the most effective and inexpensive method for restoring the genetic resources of extinct animal breeds. Semen freezing technology plays a crucial role in the fields of animal genetic resource diversity protection, introduction of breeding stock, and variety cultivation. Cryopreservation of semen in straws is currently the best solution.

[0003] Currently, there are generally two methods for the freezing device of cryopreserved semen in straws. One is a commercial programmed freezer, which is complex and expensive, not convenient to carry and use under the relatively poor conditions of breeding farms. The other method is a simple liquid nitrogen fumigation method, usually using a thermos box filled with liquid nitrogen to freeze the straw semen in the nitrogen vapor phase. At different heights from the liquid nitrogen surface, the concentration of nitrogen vapor is different. The closer to the liquid nitrogen surface, the higher the gas concentration and the lower the temperature. By selecting the appropriate combination of height and residence time for the straw semen to park, the effect of freezing at any freezing rate can be obtained.

[0004] However, in the existing simple device technology, generally a lifting system for placing straw semen (including a series of structures such as linkage, transmission, and gears) is used to bring the straw closer to the liquid nitrogen surface. These transmission structures and materials are placed above the nitrogen vapor or even in contact with the nitrogen vapor. When receiving ultra-low temperature thermal fusion, they are prone to frosting, requiring high material and technical requirements, being difficult to implement, and easily damaged. When using a lightweight material such as a foam box for freezing, there are also problems such as easy slipping of the straws, and difficulties in laying and transferring the straws. In addition, the liquid nitrogen fumigation method lacks a simple and feasible fully automatic programmed freezing scheme for any freezing rate. Summary of the Invention

[0005] The purpose of the present invention is to provide a portable liquid nitrogen fumigation programmed freezer.

[0006] To achieve the above object, the present invention adopts the following technical solutions: A portable liquid nitrogen fumigation program freezer, comprising a freezing box, the bottom of the freezing box is connected with an equipment box and a storage box, the bottoms of the equipment box and the storage box are connected to a base, the tops of the equipment box and the storage box are provided with first buckles arrayed, there are four first buckles, and they are respectively hinged on the left and right sides of the upper surfaces of the equipment box and the storage box, the first buckles are clamped with first slots opened at the bottoms of the front and rear sides of the freezing box, and the freezing box is fixed on the tops of the equipment box and the storage box, the upper and lower ends of the left and right sides of the storage box close to the equipment box are hinged with second buckles, the upper and lower ends of the left and right sides of the equipment box close to the storage box are provided with second slots adapted to the second buckles, the second buckles are clamped with the second slots, and the storage box is fixed on the front side of the equipment box, the bottom of the base is provided with walking wheels, the front of the freezing box is provided with a controller box, the top of the freezing box is hinged with a top cover through a hinge shaft, and a single-chip microcomputer controller is arranged in the controller box.

[0007] Preferably, the inner wall of the freezing box is provided with a first low-temperature heat preservation layer, the inner wall of the top cover is provided with a second low-temperature heat preservation layer, the inner bottom of the freezing box is provided with a support plate, the upper surface of the support plate is provided with a groove, a rectangular liquid nitrogen freezing bucket is sleeved in the groove, the top of the side wall of the freezing box is connected with an L-shaped bracket, the horizontal frame at the lower end of the L-shaped bracket supports a first thin tube rack, the lower surface of the support plate is fixedly connected with a support rod, and a temperature sensor is arranged at the upper part of the inner wall of the second low-temperature heat preservation layer.

[0008] Preferably, the tops of the left and right sides of the freezing box are respectively fixedly connected with mounting plates, the upper surfaces of the mounting plates are fixedly connected with first servo motors, the output shafts of the servo motors are fixedly connected with driving gears, the left and right sides of the top cover are respectively fixedly connected with arc-shaped adjusting rods, the arc-shaped adjusting rods are concentric with the hinge shaft, the upper ends of the arc-shaped adjusting rods are fixedly connected with the sides of the top cover, the lower ends movably penetrate through the mounting plates, and tooth patterns are arranged on the inner side walls of the arc-shaped adjusting rods and are meshed with the driving gears.

[0009] Preferably, the surface of the arc-shaped adjusting rod is provided with arc scales.

[0010] Preferably, the lower end of the support rod slidably penetrates through the inner bottom wall of the freezer and the top wall of the equipment box and extends into the equipment box. A reverse-F-shaped mounting bracket is fixedly connected inside the equipment box. A first threaded rod is rotatably connected inside the reverse-F-shaped mounting bracket. The top end of the first threaded rod is rotatably connected to the inner top wall of the equipment box, and the lower end rotatably penetrates through the upper cross plate of the reverse-F-shaped mounting bracket. A second servo motor is arranged below the reverse-F-shaped mounting bracket. The output shaft of the second servo motor rotatably penetrates through the lower cross plate of the reverse-F-shaped mounting bracket and is drivingly connected to the lower end of the first threaded rod. A first sliding table is threadedly sleeved on the surface of the first threaded rod. The upper surface of one end of the first sliding table is rotatably connected to the lower end of the support rod, and the other end is slidably lapped with the inner wall of the reverse-F-shaped mounting bracket.

[0011] Preferably, a displacement sensor is arranged on the reverse-F-shaped mounting bracket. The displacement sensor and the second servo motor are wirelessly connected to the controller box or electrically connected to the controller box (5) through a second wire. The end of the second wire away from the second servo motor movably penetrates through the side wall of the equipment box and is connected with a second plug.

[0012] Preferably, the first thin tube rack includes a rectangular ring plate provided with a rectangular inner hole. Protective vertical plates are arranged on both sides of the upper surface of the rectangular ring plate. Thin tube placement rack plates are arranged on both sides of the upper surface of the rectangular ring plate and inside the rectangular inner hole. A plurality of arc-shaped through grooves are arranged at the top of the thin tube placement rack plates. The two thin tube placement rack plates are parallel to the protective vertical plates. The top end of the protective vertical plate is higher than the top end of the thin tube placement plate rack. "┌┐"-shaped support plates are fixedly connected to both sides of the lower surface of the rectangular ring plate. The support plates are perpendicular to the protective vertical plates. The width of the "┌┐"-shaped support plate is equal to the distance value between the opposite surfaces of the two protective vertical plates. Protrusions are arranged on both sides of the "┌┐"-shaped support plate. Handle plates are arranged at both ends of the rectangular ring plate. The length of the handle plate is less than the distance value between the opposite inner walls of the "┌┐"-shaped support plate. Two weight-reducing holes are symmetrically arranged on the handle plate.

[0013] As another preferred solution, a third servo motor is arranged at one end of the top of the freezer. The output shaft of the third servo motor rotatably penetrates through the top wall of the freezer and the first low-temperature insulation layer and is connected with a second threaded rod. An L-shaped mounting bracket is fixedly connected to the inner side wall of the freezer. The lower end of the second threaded rod is rotatably connected to the lower cross plate of the L-shaped mounting bracket. A second sliding table is threadedly sleeved on the surface of the second threaded rod. The surface of the second sliding table is slidably lapped with the inner wall of the L-shaped mounting bracket. The end of the second sliding table away from the L-shaped mounting bracket is fixedly connected to a support plate.

[0014] As another preferred solution, a second thin tube holder is supported on the horizontal frame at the lower end of the L-shaped bracket. The second thin tube holder includes a rectangular ring block and an annular convex platform arranged on the top of the rectangular ring block. A thin tube placement groove is provided at the top of the inner wall of the annular convex platform. A storage groove adapted to the annular convex platform is provided on the lower surface of the rectangular ring block. A limiting card slot is provided on the side of the second thin tube holder close to the L-shaped bracket.

[0015] As another preferred solution, the second thin tube holder further includes a thin tube scrubbing plate, and scrubbing plate arc grooves adapted to the thin tubes are uniformly arranged on the upper surface of the thin tube scrubbing plate.

[0016] Beneficial effects

[0017] 1. In the thin tube semen freezing system of the present invention, by providing an independent freezing box and an equipment box, the freezing chamber is isolated from the transmission mechanism electronic control system, avoiding the dilemma of frosting of the transmission mechanism due to low temperature, thereby prolonging the service life of the equipment. By setting the freezing box, the equipment box, the storage box and the base as independent components, it is convenient to disassemble and store when not in use, convenient to assemble and combine when needed, and convenient to be packaged and transported to different animal resource farms to implement on-site freezing of thin tube semen. By controlling the opening degree of the top cover, a high temperature of about 4°C is obtained, avoiding difficulties in obtaining high temperature caused entirely by the increase in the distance from the liquid nitrogen surface; by designing a drawer-type storage box to classify and store various thin tube semen freezing utensils, avoiding dilemmas such as spending a long time cleaning and packing when mailing experimental utensils; by providing a detachable L-shaped bracket to support the first thin tube holder, it is convenient to put in and remove the thin tube holder, realizing convenient immersion of the thin tube into liquid nitrogen, and conveniently transferring the container containing the thin tube and liquid nitrogen, realizing functions such as operating the thin tube in liquid nitrogen, performing quality inspection, and storing in a bag after the inspection is completed; by installing a detachable ultra-low temperature temperature monitoring device on the thin tube holder support or connecting an electric device controlled according to the real-time temperature, it is convenient to monitor the freezing process and realize freezing at any freezing rate.

[0018] 2. In the thin tube semen freezing system of the present invention, the thin tubes placed in the freezing box remain stationary. By providing a lifting device on the bottom plate of the liquid nitrogen container to control the lifting of the liquid nitrogen container, and then controlling the distance between the top surface of the liquid nitrogen and the thin tubes, the freezing of thin tube semen is realized, avoiding the risk of the thin tube semen slipping caused by lifting the thin tube semen. By providing the first and second thin tube holders, it is convenient to limit the thin tubes on the thin tube holders and keep the thin tube semen stable. By providing the L-shaped bracket, it is convenient to support the thin tube holder above the liquid nitrogen container.

[0019] 3. The fine-tube semen freezing system of the present invention facilitates the stacking of the first fine-tube racks through the cooperation of the bumps on the upper-layer first fine-tube racks and the protective vertical plates on the lower-layer first fine-tube racks. By providing two "┌┐"-shaped support plates with a spacing at both ends of the bottom of the first fine-tube rack that is adapted to the width of the L-shaped bracket, it is convenient to limit the L-shaped bracket on the top of the first fine-tube rack, enhancing the stability of the first fine-tube rack on the L-shaped bracket.

[0020] 4. The fine-tube semen freezing system of the present invention facilitates the restriction of the fine tubes on the second fine-tube rack by providing a fine-tube placement groove at the top of the second fine-tube rack, preventing the fine tubes from slipping off the second fine-tube rack.

[0021] 5. The fine-tube semen freezing system of the present invention enhances the stability of the second fine-tube rack on the L-shaped bracket by providing a limit card slot on the side of the second fine-tube rack that is adapted to the L-shaped bracket, and the L-shaped bracket is slidably clamped in the limit card slot.

[0022] 6. The fine-tube semen freezing system of the present invention facilitates the quick and neat arrangement of a batch of fine tubes by providing a fine-tube rubbing plate. Through the cooperation of the fine-tube rubbing plate and the second fine-tube rack, it is convenient to quickly and neatly lay a batch of fine tubes on the second fine-tube rack, improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 is a schematic structural diagram of the present invention;

[0024] Figure 2 is a schematic diagram of the dispersed structure of the present invention;

[0025] Figure 3 is a side view of the present invention;

[0026] Figure 4 is a schematic side-sectional structure diagram of the present invention;

[0027] Figure 5 is a schematic top-sectional structure diagram of the freezer in the present invention;

[0028] Figure 6 is Figure 4 an enlarged view of part A in

[0029] Figure 7 is a schematic diagram of the connection state of the arc-shaped adjusting rod and the top cover in the present invention;

[0030] Figure 8 is a schematic diagram of the connection state of the first buckle and the freezer in the present invention;

[0031] Figure 9 is a schematic diagram of the structure of the L-shaped bracket in the present invention;

[0032] Figure 10 is a schematic diagram of the structure of the first fine-tube rack in the present invention;

[0033] Figure 11 It is a side view of the first thin tube rack in the present invention;

[0034] Figure 12 It is a schematic diagram of the superposed state of the first thin tube rack in the present invention;

[0035] Figure 13 It is a schematic diagram of the state when the first thin tube rack places thin tubes in the present invention;

[0036] Figure 14 It is a schematic structural diagram of the second embodiment in the present invention;

[0037] Figure 15 It is a side view of the second embodiment in the present invention;

[0038] Figure 16 It is a schematic structural diagram of the second thin tube rack in the present invention;

[0039] Figure 17 It is a schematic bottom view structure diagram of the second thin tube rack in the present invention;

[0040] Figure 18 It is a schematic diagram of the superposed state of the second thin tube rack in the present invention;

[0041] Figure 19 It is a schematic structural diagram of the thin tube scrubbing board in the present invention;

[0042] Figure 20 It is a schematic diagram of the state when thin tubes are placed on the thin tube scrubbing board in the present invention;

[0043] Figure 21 It is a schematic structural diagram of the second thin tube rack when the thin tube placement groove of the second thin tube rack is a wide groove for placing multiple thin tubes in the present invention;

[0044] Figure 22 It is a schematic diagram of the superposed state of the second thin tube rack in the form of a wide groove in the present invention.

[0045] In the figure: 1, freezer; 2, equipment box; 3, storage box; 4, base; 5, controller box; 101, top cover; 102, first low-temperature insulation layer; 103, second low-temperature insulation layer; 104, pallet; 105, rectangular liquid nitrogen freezer barrel; 106, L-shaped bracket; 107, first thin tube rack; 108, hinge shaft; 109, mounting plate; 110, first servo motor; 111, driving gear; 112, arc-shaped adjusting rod; 113, first card slot; 114, annular card slot; 115, annular clamping block; 116, rectangular limit slot; 117, "┌" - shaped clamping plate; 118, temperature sensor; 119, first wire; 120, first plug; 121, third servo motor; 122, second threaded rod; 123, L-shaped mounting frame; 124, second sliding table; 125, second thin tube rack; 126, rectangular ring block; 127, annular boss; 128, thin tube placement groove; 129, storage groove; 130, limit card slot; 131, thin tube scrubbing board; 132, scrubbing board arc-shaped groove; 133, thin tube; 134, heat insulation curtain; 201, support rod; 202, inverted F-shaped mounting frame; 203, first threaded rod; 204, second servo motor; 205, first sliding table; 206, first buckle; 207, second card slot; 208, second wire; 209, second plug; 301, second buckle; 302, drawer; 401, walking wheel; 501, single-chip microcomputer controller; 502, display screen; 503, controller button; 504, second interface; 1071, rectangular ring plate; 1072, protection vertical plate; 1073, thin tube placement shelf board; 1074, arc-shaped through groove; 1075, "┌┐" - shaped support plate; 1076, bump; 1077, handle plate. Detailed implementation mode

[0046] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0047] Embodiment 1:

[0048] As Figure 1-13As shown in the figure, the present invention provides a technical solution: a portable liquid nitrogen fumigation program freezer, including that the bottom of the freezer 1 is connected to an equipment box 2 and a storage box 3. The equipment box 2 and the storage box 3 are connected in parallel at the bottom of the freezer 1. The bottoms of the equipment box 2 and the storage box 3 are connected in parallel in a groove opened on a base 4. The tops of the equipment box 2 and the storage box 3 are provided with first buckles 206 in an array. There are four first buckles 206, which are respectively hinged on the left and right sides of the upper surfaces of the equipment box 2 and the storage box 3. The first buckles 206 are clamped with first slots 113 opened at the bottoms of the front and rear sides of the freezer 1, and the freezer 1 is fixed on the tops of the equipment box 2 and the storage box 3. The upper and lower ends of the left and right sides of the storage box 3 close to the equipment box 2 are hinged with second buckles 301. Second slots 207 adapted to the second buckles 301 are opened at the upper and lower ends of the left and right sides of the equipment box 2 close to the storage box 3. The second buckles 301 are clamped with the second slots 207, and the storage box 3 is fixed on the front side of the equipment box 2. A plurality of drawers 302 are successively arranged on the front side of the storage box 3 from top to bottom, which is convenient for classifying and storing a variety of cryogenic instruments for fine tube semen, avoiding the dilemmas such as spending a long time cleaning and packing when mailing experimental instruments. The bottom of the base 4 is provided with traveling wheels 401, and the traveling wheels 401 are provided with a braking device. The front of the freezer 1 is provided with a controller box 5. The top of the freezer 1 is hinged with a top cover 101 through a hinge shaft 108. A single-chip microcomputer controller 501 is arranged in the controller box 5. A display screen 502 is arranged on the top of the controller box 5. The display screen 502 is inclined and installed on the top of the controller box 5. Controller buttons 503 are arranged on the front of the controller box 5. A first interface adapted to a first plug 120 and a second interface 504 adapted to a second plug 209 are arranged on the side of the controller box 5. The first plug 120 is clamped in the first interface opened on the side of the controller box 5. The second plug 209 is clamped in the second interface 504 opened on the side of the controller box 5. The single-chip microcomputer controller 501 is electrically connected to a temperature sensor 118, a first servo motor 110, a second servo motor 204, a displacement sensor and the display screen 502 respectively. By setting up an independent freezer 1 and an equipment box 2, and arranging the controller box 5 outside the freezer 1, the freezer is isolated from the electronic control system, which is convenient for extending the service life of the equipment. By setting up a controllable top cover 101 and controlling the opening degree of the top cover 101, a high temperature of about 4 °C can be obtained. By setting the freezer 1, the equipment box 2, the storage box 3 and the base 4 as independent components, it is convenient to disassemble, pack and transport when not in use, and it is convenient to assemble and combine when needed.

[0049] The inner wall of the freezer 1 is provided with a first low-temperature insulation layer 102, and the inner wall of the top cover 101 is provided with a second low-temperature insulation layer 103. The lower surface of the second low-temperature insulation layer 103 overlaps the top of the first low-temperature insulation layer 102. An annular slot 114 is formed in the top of the first low-temperature insulation layer 102. The lower surface of the second low-temperature insulation layer 103 is provided with a protruding annular block 115 that matches the annular slot 114. The cross-section of the annular slot 114 is arc-shaped or inverted trapezoidal. The inner bottom of the freezer 1 is provided with a tray 104. The upper surface of the tray 104 is provided with a groove, and a rectangular liquid nitrogen freezing bucket 105 with an open top is sleeved in the groove. Both sides of the rectangular liquid nitrogen freezing bucket 105 are provided with handles for easy taking and placing of the rectangular liquid nitrogen freezing bucket 105. The rectangular liquid nitrogen freezing bucket 105 contains liquid nitrogen for freezing animal semen. The top of the side wall of the freezer 1 is connected with an L-shaped bracket 106, which is equipped with an ultra-low temperature thermocouple temperature detection line and a probe that are easy to disassemble. The ultra-low temperature temperature probe is wirelessly connected to the single-chip microcomputer controller 501, and the detected temperature information is displayed on the display screen 502. The top of the side wall of the freezer 1 is provided with a rectangular limit slot 116. The top of the L-shaped bracket 106 is provided with a "┌"-shaped clamping plate 117, and the "┌"-shaped clamping plate 117 is clamped in the rectangular limit slot 116. The lower end of the "┌"-shaped clamping plate 117 is provided with a chamfer to facilitate the insertion of the "┌"-shaped clamping plate 117 into the rectangular limit slot 116. The lower crossbar of the L-shaped bracket 106 supports a first thin tube rack 107, which is used for neatly placing thin tubes for freezing animal semen. The length and width of the lower end of the L-shaped bracket 106 and the length and width of the first thin tube rack 107 are both smaller than the length and width of the open top of the rectangular liquid nitrogen freezing bucket 105. The lower surface of the tray 104 is fixedly connected with a support rod 201, and the surface of the support rod 201 is provided with a low-temperature insulation protective layer to facilitate reducing the heat conduction of the support rod 201 and reducing the influence on the temperature of the freezer 1. The center of the lower surface of the tray 104 is fixedly connected with a threaded sleeve, and the top of the support rod 201 is provided with an external thread and is threadedly connected to the threaded sleeve. The upper part of the inner wall of the second low-temperature insulation layer 103 is provided with a temperature sensor 118, and the temperature sensor 118 is wirelessly connected to the controller box 5 or electrically connected to the controller box 5 through a wire.

[0050] The tops of the left and right sides of the freezer 1 are respectively fixedly connected with mounting plates 109, the upper surface of the mounting plate is fixedly connected with a first servo motor 110, the output shaft of the servo motor 110 is fixedly connected with a driving gear 111, the left and right sides of the top cover 101 are respectively fixedly connected with arc-shaped adjustment rods 112, the arc-shaped adjustment rods 112 are cocentrically arranged with the hinge shaft 108, the upper end of the arc-shaped adjustment rod 112 is fixedly connected with the side of the top cover 101, and the lower end movably penetrates the mounting plate 109, and the inner wall of the arc-shaped adjustment rod 112 It is provided with teeth and meshes with the driving gear 111, the first servo motor 110 is wirelessly connected to the controller box 5 or electrically connected to the controller box 5 through the first wire 119, and the first wire 119 is provided with a first plug connector 120 at one end away from the first servo motor 110. Through the cooperation of the first servo motor 110 and the first servo motor 110, it is convenient to arbitrarily adjust the opening amplitude of the top cover 101 within the range of 0-90°, so as to obtain a high temperature of about 4°C and avoid the difficulties of obtaining a high temperature caused by the increase in the distance from the liquid nitrogen surface.

[0051] The surface of the arc-shaped adjusting rod 112 is provided with an arc scale. When the top cover 101 is closed on the top of the freezer 1, the 0 mark of the arc scale is flush with the top of the freezer 1. By observing the arc scale on the surface of the arc-shaped adjusting rod 112, the opening angle of the top cover 101 can be quickly and accurately known.

[0052] The lower end of the support rod 201 slides through the inner bottom wall of the freezer 1 and the top wall of the equipment box 2 and extends into the equipment box 2. An inverted F-shaped mounting frame 202 is fixedly connected to the equipment box 2. A first threaded rod 203 is rotatably connected to the inverted F-shaped mounting frame 202. The top end of the first threaded rod 203 is rotatably connected to the inner top wall of the equipment box 2 through a bearing or a slewing bearing, and the lower end rotatably penetrates the upper horizontal plate of the inverted F-shaped mounting frame 202. A second servo motor 204 is provided below the inverted F-shaped mounting frame 202. The second servo motor 204 is 4 is a forward and reverse servo motor, the output shaft of the second servo motor 204 rotates and penetrates the lower horizontal plate of the inverted F-shaped mounting frame 202 and is drivingly connected to the lower end of the first threaded rod 203, the surface of the first threaded rod 203 is threadedly sleeved with a first slide 205, the upper surface of one end of the first slide 205 is rotatably connected to the lower end of the support rod 201, and the other end is slidably overlapped with the inner wall of the inverted F-shaped mounting frame 202, and the inner wall of the inverted F-shaped mounting frame 202 and the end surface of the first slide 205 away from the support rod 201 are both smooth surfaces.

[0053] A displacement sensor is provided on the inverted F-shaped mounting bracket 202. The displacement sensor and the second servo motor 204 are wirelessly connected to the controller box 5 or electrically connected to the controller box 5 through the second wire 208. One end of the second wire 208 away from the second servo motor 204 movably penetrates the side wall of the equipment box 2 and is connected with a second plug 209. By providing the displacement sensor, it is convenient to detect the up and down movement distance of the first sliding table 205, thereby knowing the lifting height of the rectangular liquid nitrogen freezing barrel 105, and displaying this information on the display screen 502.

[0054] The first capillary tube rack 107 includes a rectangular ring plate 1071 provided with a rectangular inner hole. On both sides of the upper surface of the rectangular ring plate 1071, there are protective vertical plates 1072. The two protective vertical plates 1072 are parallel, and the two ends of the protective vertical plates 1072 protrude from the rectangular ring plate 1071. On both sides of the upper surface of the rectangular ring plate 1071 and inside the rectangular inner hole, there are capillary tube placement shelf plates 1073. At the top of the capillary tube placement shelf plates 1073, there are several arc-shaped through grooves 1074 for placing capillary tubes. The two capillary tube placement shelf plates 1073 are parallel to the protective vertical plates 1072. The top end of the protective vertical plates 1072 is higher than the top end of the capillary tube placement shelf plates 1073, and the height difference between the top end of the protective vertical plates 1072 and the top end of the capillary tube placement shelf plates 1073 is greater than the diameter of the capillary tube. On both sides of the lower surface of the rectangular ring plate 1071, there are fixedly connected "┌┐"-shaped support plates 1075. The support plates are perpendicular to the protective vertical plates 1072. The width of the "┌┐"-shaped support plates 1075 is equal to the distance value between the opposite surfaces of the two protective vertical plates 1072. There are convex blocks 1076 on both sides of the "┌┐"-shaped support plates 1075. When the first capillary tube racks 107 are arranged in multiple layers, the convex blocks 1076 are movably lapped on the top of the protective vertical plates 1072 of the first capillary tube rack 107 on the next lower layer. At both ends of the rectangular ring plate 1071, there are handle plates 1077. The length of the handle plates 1077 is less than the distance value between the opposite inner walls of the "┌┐"-shaped support plates 1075. There are two symmetrically arranged weight reduction holes on the handle plates 1077.

[0055] The usage process of the present invention is as follows: First, place the equipment box 2 and the storage box 3 side by side in the groove opened on the base 4. Then, snap the second buckle 301 hinged on the side of the storage box 3 into the second slot 207 opened on the side of the equipment box 2 to fix the storage box 3 on the front side of the equipment box 2. Then, place the freezer 1 on the tops of the equipment box 2 and the storage box 3. Then, snap the first buckle 206 on the tops of the equipment box 2 and the storage box 3 with the first slots 113 opened at the bottoms of the front and rear sides of the freezer 1 to fix the freezer 1 on the tops of the equipment box 2 and the storage box 3. Connect the first plug 120 on the first wire 119 to the first interface on the side of the controller box 5, and connect the second plug 209 on the second wire 208 to the second interface 504 on the side of the controller box 5, so that the first servo motor 110, the second servo motor 204 and the displacement sensor are connected to the single-chip microcomputer controller 501 and receive control instructions. Move the support rod 201 upward so that the top end of the support rod 201 extends into the freezer 1 and is fixedly connected to the bottom of the tray 104. Open the top cover 101, take out the rectangular liquid nitrogen freezing barrel 105 in the freezer 1 and inject liquid nitrogen into it. Then, put the rectangular liquid nitrogen freezing barrel 105 back on the tray 104 in the freezer 1. (It is also possible not to take out the rectangular liquid nitrogen freezing barrel 105 and directly inject liquid nitrogen into the rectangular liquid nitrogen freezing barrel 105. When injecting liquid nitrogen, set a foam buoy in the rectangular liquid nitrogen freezing barrel 105, set a scale on the top of the foam buoy, and the foam buoy will float up after pouring in liquid nitrogen and stop injecting liquid nitrogen when it floats to the specified mark). Place the first capillary tube rack 107 neatly placed with capillary tubes 133 on the L-shaped bracket 106. Then, snap the "┌"-shaped clamping plate 117 at the top of the L-shaped bracket 106 into the rectangular limit slot 116 at the top of the inner wall of the freezer 1, so as to fix the L-shaped bracket 106 above the rectangular liquid nitrogen freezing barrel 105 in the freezer 1. Place the first capillary tube rack 107 with capillary tube semen on the L-shaped bracket 106. Then, start the second servo motor 204 to drive the first threaded rod 203 to rotate forward, thereby driving the first slide 205 to move upward. The first slide 205 drives the support rod to move upward, thereby driving the rectangular liquid nitrogen freezing barrel 105 to move upward to an appropriate position, and then the capillary tube semen can be frozen. This portable liquid nitrogen fumigation program freezer can set the freezing program through the temperature sensor, or can also set the freezing program according to the speed and time. Taking the freezing of chicken glycerol capillary tube semen as an example, usually first cool the capillary tube semen to 5 °C, then drop from 5 °C to -35 °C at a rate of -7 °C / min, then drop from -35 °C to -140 °C at a rate of -60 °C / min, and then immediately immerse the capillary tube in liquid nitrogen and stay for at least 15 minutes or more to complete the freezing of the capillary tube semen;The portable liquid nitrogen fumigation program freezer of the present invention sets up an independent freezer and an equipment box, and sets the controller box outside the freezer, isolating the freezer compartment from the electronic control system and the mechanical transmission device, which is convenient for reducing the technical difficulty of equipment development and extending the service life of the equipment. By setting the freezer, equipment box, storage box and base as independent components, it is convenient to disassemble, pack and transport when not in use, and to assemble and combine when needed, and it is convenient to transport to different animal resource farms for on-site freezing of straw semen. By controlling the opening degree of the top cover, a high temperature of about 4°C can be obtained, avoiding difficulties in obtaining high temperature caused entirely by the increase in the distance from the liquid nitrogen surface. By designing a drawer-type storage box to store various straw semen freezing utensils in categories, it avoids difficulties such as spending a long time cleaning and packing when mailing experimental utensils. By setting a detachable L-shaped bracket to support the first straw rack, it is convenient to put in and remove the straw rack, realizing convenient immersion of the straw into liquid nitrogen, and conveniently transferring the container containing the straw and liquid nitrogen, and realizing functions such as operating the straw in liquid nitrogen for quality detection and storing it in a bag after the detection. By installing a cryogenic temperature monitoring device that is easy to disassemble on the straw rack support, or connecting an electric device controlled according to the real-time temperature, it is convenient to monitor the freezing program and realize freezing at any freezing rate. At the same time, the straw placed in the freezer remains stationary. By setting a lifting device on the bottom plate of the liquid nitrogen container to control the lifting of the liquid nitrogen container, and then controlling the distance between the liquid nitrogen surface and the straw, the freezing of straw semen is realized, avoiding the risk of the straw semen slipping caused by lifting the straw semen. By setting the first and second straw racks, it is convenient to select a matching freezing device and freezing rack according to the freezing program required by a specific freezing example, simplifying the freezing system. By setting an L-shaped bracket, it is convenient to support the straw rack above the liquid nitrogen container.;

[0056] Embodiment 2:

[0057] As Figure 14-15As shown in the figure, a portable liquid nitrogen fumigation program freezer includes a freezer box 1 and a storage box 3 stacked up and down. The lower end of the storage box 3 is placed in a groove on the top of a base 4. Four first buckles 206 are provided on the top of the storage box 3. The first buckles 206 are clamped with first card slots 113 opened at the bottom of the front and rear sides of the freezer box 1 to fix the freezer box 1 on the top of the storage box 3. A top cover 101 is hinged to the top of the freezer box. A first low-temperature heat preservation layer 102 is provided on the inner wall of the freezer box 1, and a second low-temperature heat preservation layer 103 is provided on the inner wall of the top cover 101. A third servo motor 121 is provided at one end of the top of the freezer box 1. The third servo motor 121 is a forward and reverse servo motor. The output shaft of the third servo motor 121 rotates through the top wall of the freezer box 1 and the first low-temperature heat preservation layer 102 and is connected to a second threaded rod 122. A L-shaped mounting bracket 123 is fixedly connected to the inner side wall of the freezer box 1. The lower end of the second threaded rod 122 is rotationally connected to the lower horizontal plate of the L-shaped mounting bracket 123 through a bearing or a slewing bearing. A second sliding table 124 is threadedly sleeved on the surface of the second threaded rod 122. The surface of the second sliding table 124 is slidably lapped with the inner wall of the L-shaped mounting bracket 123. One end of the second sliding table 124 away from the L-shaped mounting bracket 123 is fixedly connected to a support plate 104. The inner wall of the L-shaped mounting bracket 123 and the end surface of one end of the second sliding table 124 away from the support plate 104 are both smooth surfaces. The L-shaped mounting bracket 123 is embedded in the first low-temperature heat preservation layer 102. A heat insulation curtain 134 that can be folded in a wave shape is provided between the second threaded rod 122 and the support plate 104. The top end of the heat insulation curtain 134 is hermetically connected to the inner top wall of the freezer box 1, and the lower end is hermetically connected to the inner bottom wall of the freezer box 1. The two side edges of the heat insulation curtain 134 are slidably attached to the inner wall of the freezer box 1. The second sliding table 124 passes through the heat insulation curtain 134 and is fixedly connected to the support plate 104. By providing the heat insulation curtain 134 that can be folded in a wave shape to isolate the freezer from the driving equipment, the adverse effects of low temperature on the equipment are reduced, and the service life of the equipment is improved. The other structures are the same as those in Embodiment 1.

[0058] Embodiment 3:

[0059] As Figure 16-20As shown in the figure, a portable liquid nitrogen fumigation program freezer includes a device box 2 and a storage box 3 connected to the bottom of a freezing box 1. The device box 2 and the storage box 3 are connected in parallel to the bottom of the freezing box 1, and the bottoms of the device box 2 and the storage box 3 are connected in parallel to the grooves opened on a base 4. The top of the side wall of the freezing box 1 is connected with an L-shaped bracket 106, and a second thin tube rack 125 is supported on the horizontal rack at the lower end of the L-shaped bracket 106. In this embodiment, the second thin tube rack 125 is made of foam material. The second thin tube rack 125 includes a rectangular ring block 126 and an annular boss 127 arranged on the top of the rectangular ring block 126. At the top of the inner walls on both sides of the annular boss 127, thin tube placement grooves 128 adapted to thin tubes 133 are opened. In this embodiment, the thin tube placement grooves 128 are narrow grooves for placing a single thin tube. The lower surface of the rectangular ring block 126 is provided with a storage groove 129 adapted to the annular boss 127. When multiple layers of the second thin tube racks 125 are arranged, the annular boss 127 of the lower-layer second thin tube rack 125 is snapped into the storage groove 129 at the bottom of the upper-layer second thin tube rack 125. A limit card slot 130 is opened on the side of the second thin tube rack 125 close to the L-shaped bracket 106, and the vertical plate of the L-shaped bracket 106 is slidably snapped into the limit card slot 130 to enhance the stability of the second thin tube rack 125 on the L-shaped bracket 106. The second thin tube rack 125 further includes a thin tube rubbing plate 130. The upper surface of the thin tube rubbing plate 130 is evenly provided with rubbing plate arc grooves 132 adapted to the thin tubes 133. The height of the rubbing plate arc grooves 132 is higher than that of the second thin tube rack 125, and the distance between the rubbing plate arc grooves 132 is equal to the distance between the thin tube placement grooves 128. When in use, first place the thin tube rubbing plate 130 in the second thin tube rack 125, then place a plurality of thin tubes 133 containing animal semen at one end of the thin tube rubbing plate 130, with the thin tubes 133 parallel to the rubbing plate arc grooves 132, and then rub the thin tubes 133 towards the other end of the thin tube rubbing plate 130 to unfold the thin tubes 133 and evenly snap them into the rubbing plate arc grooves 132, so that the thin tubes 133 containing animal semen are neatly arranged. Then lift the second thin tube rack 125 upwards, and both ends of the neatly arranged thin tubes 133 respectively fall into the thin tube placement grooves 128 on the inner walls on both sides of the second thin tube rack 125, and the neatly arranged thin tubes 133 can be batch-transferred to the second thin tube rack 125 and then placed in the freezing box 1 for freezing. The second thin tube rack 125 can either replace the first thin tube rack 107 or be used independently. Since the second thin tube rack 125 is made of foam, it has a small density and a light weight and directly floats on the liquid nitrogen. By stacking the second thin tube racks 125, the distance (height) of the thin tubes 133 relative to the liquid nitrogen liquid surface can be selected to implement thin tube freezing. The second thin tube rack 125 can also be detached from this system and used in a foam box filled with liquid nitrogen. The other structures of this embodiment are the same as those of Embodiment 1 or 2.

[0060] As an alternative, as Figure 21-22 shown, two or more wide grooves are provided at the top of the second thin tube rack 125, and a plurality of thin tube placement grooves 128 are provided in the wide grooves.

[0061] It should be noted that in this text, terms such as "including", "comprising", or any other variants thereof are intended to cover non-exclusive inclusion. Thus, a process, method, article, or device that includes a series of elements not only includes those elements but also includes other elements not explicitly listed, or elements that are inherent to such a process, method, article, or device. The terms "first" and "second" are only used for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include at least one of such features. Unless otherwise clearly specified and defined, the terms "installed", "connected", and "coupled" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0062] Although the embodiments of the present invention have been shown and described, those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes, modifications, substitutions, and variations, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A portable liquid nitrogen fumigation process freezer, comprising a freezer box, Features: The bottom of the freezer is connected to an equipment box and a storage box, the bottoms of the equipment box and the storage box are connected to the base, the top arrays of the equipment box and the storage box are provided with first buckles, four first buckles are provided, and are respectively hinged on the left and right sides of the upper surfaces of the equipment box and the storage box, the first buckles are engaged with first buckle slots opened at the bottom of the front and rear side surfaces of the freezer, and the freezer is fixed to the top of the equipment box and the storage box, the left and right sides of the storage box are hinged with second buckles at the upper and lower ends close to one side of the equipment box, the left and right sides of the storage box are provided with second buckles adapted to the second buckles at the upper and lower ends close to one side of the storage box, the second buckles are engaged with the second buckles, and the storage box is fixed to the front side of the equipment box, the bottom of the base is provided with walking wheels, the front of the freezer is provided with a controller box, the top of the freezer is hinged with a top cover through a hinge shaft, and a single-chip controller is provided in the controller box; The bottom of the freezing box is provided with a support plate, the upper surface of the support plate is provided with a groove, and a rectangular liquid nitrogen freezing barrel is sleeved in the groove, and the lower surface of the support plate is fixedly connected to a support rod, and the lower end of the support rod slides through the inner bottom wall of the freezing box and the top wall of the equipment box and extends into the equipment box, and is fixedly connected to an inverted F-shaped mounting frame in the equipment box, and a first threaded rod is rotatably connected in the inverted F-shaped mounting frame, the top end of the first threaded rod is rotatably connected to the inner top wall of the equipment box, and the lower end rotatably penetrates the upper cross plate of the inverted F-shaped mounting frame, and a second servo motor is provided below the inverted F-shaped mounting frame, and the output shaft of the second servo motor rotates through the lower cross plate of the inverted F-shaped mounting frame and is transmission connected to the lower end of the first threaded rod, and a first slide is threadedly sleeved on the surface of the first threaded rod, and the upper surface of one end of the first slide is rotatably connected to the lower end of the support rod, and the other end is slidably overlapped with the inner wall of the inverted F-shaped mounting frame; An L-shaped bracket is connected to the top of the side wall of the freezer, and a first capillary rack is supported on the frame at the lower end of the L-shaped bracket; The first capillary tube rack comprises a rectangular ring plate with a rectangular inner hole, protective vertical plates are provided on both sides of the upper surface of the rectangular ring plate, capillary tube placement rack plates are provided on the upper surface of the rectangular ring plate and on both sides of the rectangular inner hole, and a plurality of arc-shaped through grooves are provided on the top of the capillary tube placement rack plate.

2. A portable liquid nitrogen fumigation program freezer according to claim 1, Features: The inner wall of the freezer is provided with a first low-temperature thermal insulation layer, the inner wall of the top cover is provided with a second low-temperature thermal insulation layer, and a temperature sensor is provided on the upper part of the inner wall of the second low-temperature thermal insulation layer.

3. A portable liquid nitrogen fumigation program freezer according to claim 1, Features: Installation plates are respectively and fixedly connected to the tops of the left and right sides of the freezer. A first servo motor is fixedly connected to the upper surface of the installation plate. A driving gear is fixedly connected to the output shaft of the first servo motor. Arc-shaped adjusting rods are respectively and fixedly connected to the left and right sides of the top cover. The arc-shaped adjusting rods are concentric with the hinge shaft. The upper end of the arc-shaped adjusting rod is fixedly connected to the side of the top cover, and the lower end movably penetrates through the installation plate. Tooth patterns are provided on the inner side wall of the arc-shaped adjusting rod and mesh with the driving gear.

4. A portable liquid nitrogen fumigation program freezer according to claim 3, characterized in that: Arc scales are provided on the surface of the arc-shaped adjusting rod.

5. A portable liquid nitrogen fumigation program freezer according to claim 4, characterized in that: A displacement sensor is provided on the inverted F-shaped mounting bracket. The displacement sensor and the second servo motor are wirelessly connected to the controller box or electrically connected to the controller box through a second wire. One end of the second wire away from the second servo motor movably penetrates through the side wall of the equipment box and is connected with a second plug.

6. A portable liquid nitrogen fumigation program freezer according to claim 1, characterized in that: The two thin tube placement plates are parallel to the protection vertical plate. The top of the protection vertical plate is higher than the top of the thin tube placement plate. "┌┐”-shaped support plates are fixedly connected to both sides of the lower surface of the rectangular ring plate. The support plates are perpendicular to the protection vertical plate. The width of the "┌┐”-shaped support plate is equal to the distance value between the opposite surfaces of the two protection vertical plates. Protrusions are provided on both sides of the "┌┐”-shaped support plate. Handle plates are provided at both ends of the rectangular ring plate. The length of the handle plate is less than the distance value between the opposite inner surfaces of the "┌┐”-shaped support plate. Two weight reduction holes are symmetrically provided on the handle plate.

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