A method for detecting the pregnancy status of giant pandas using B-type ultrasound for non-diagnostic purposes

Abstract

The invention discloses a method for detecting the pregnancy state of a giant panda by using a B-type ultrasonic instrument for non-diagnostic purposes. One month after the giant pandas breed and mate, the ultrasonic diagnostic instrument is used to perform B-ultrasound monitoring on adult female giant pandas. Nine adult female giant pandas with an age range of 6 to 13 years old are used for monitoring in the study. Before the experiment, the pandas are systematically trained by researchers to achieve the effect of successfully cooperating with ultrasonic examinations. A total of 13 pregnancy observations are performed. Before the examination, the hair on the lower abdomen of the giant panda needs to be cut along the fetal area, and a coupling agent is applied. Then, the fetal position is detected by using ultrasonic waves, and its image can be obtained. It is verified by experiments that 15-20 days before the adult female giant pandas give birth, the giant panda fetus can be detected to be 16.85±1.34 days by B-ultrasound examination. The B-type ultrasonic instrument is successfully used to explore the abdominal wall of the giant panda to detect the pregnancy state, which is of great help to the research of problems related to the physiology of the giant panda during pregnancy.

Description

[0001] This application is a divisional application with an application date of January 2, 2020, application number 202010000756.1, and invention name "A method for detecting and diagnosing giant panda pregnancy using a B-type ultrasound device". Technical Field

[0002] The invention belongs to the technical field of artificial breeding methods for captive animals, and in particular relates to a method for detecting the pregnancy status of a giant panda by using a B-type ultrasonic instrument for non-diagnostic purposes. Background Art

[0003] Giant panda: the only mammal belonging to the order Carnivora, family Ursidae, subfamily Ailuropodinae and genus Ailuropoda, with a head and body length of 1.2-1.8 meters and a tail length of 10-12 centimeters. It weighs 80-120 kilograms, and the heaviest can reach 180 kilograms. Its body color is black and white. It has round cheeks, big dark circles under its eyes, a chubby body, a signature pigeon-toed walking style, and claws as sharp as scalpels. It is one of the cutest animals in the world.

[0004] The Aloka SSD-900V ultrasonic diagnostic instrument (Aloka Co. Ltd., Wallingford, CT) was used for B-ultrasound monitoring of adult female giant pandas, equipped with a 3.5MHz sector scanning probe. In the 1960s, B-mode ultrasonic diagnostic technology was introduced into the veterinary field, and has now developed into a B-mode ultrasonic diagnostic instrument (B-ultrasound) with two-dimensional images. B-mode ultrasound is a tomographic scan, which is a mature high-tech diagnostic technology that can observe real-time images of living organisms. There have been many reports on the application of veterinary B-ultrasound in diagnosing pregnancy in female animals, but there has been no successful case of its application in diagnosing pregnancy in giant pandas.

[0005] At the China Conservation and Research Center for the Giant Panda, medical management training for giant pandas is carried out. Through courses designed according to the special habits and abilities of giant pandas, giant pandas can cooperate with researchers' medical examinations without anesthesia (Wang Chengdong et al. Medical Management Training for Giant Pandas. Wildlife. 2012, 33(4): 246-248). Among them, training giant pandas to maintain a stable standing posture is conducive to abdominal ultrasound examination. This training is the basis for the successful use of B-type ultrasound to diagnose giant panda pregnancy. Summary of the invention

[0006] The purpose of the present invention is to provide a method for detecting and diagnosing giant panda pregnancy using a B-type ultrasound instrument to solve the problems raised in the above background technology. The present invention provides a method for detecting and diagnosing giant panda pregnancy using a B-type ultrasound instrument, which has the characteristics of being simple, understanding the giant panda's pregnancy status in real time, and being of great help to the study of issues related to the giant panda's physiology during pregnancy.

[0007] Another object of the present invention is to provide a method for implementing the method of using a B-type ultrasonic instrument to detect and diagnose giant panda pregnancy.

[0008] To achieve the above object, the present invention provides the following technical solution: a method for detecting and diagnosing giant panda pregnancy using a B-type ultrasound instrument, comprising the following steps:

[0009] (1) Baoding: Through long-term and systematic training of giant pandas by researchers, the experimental giant pandas can make postures that cooperate with B-ultrasound exploration without anesthesia;

[0010] (2) Before performing B-ultrasound examination, the panda's lower abdomen must be trimmed and a coupling agent applied;

[0011] (3) One month after mating, female giant pandas will be monitored by ultrasound to detect the position of the fetus and obtain images. This will be done monthly until uterine enlargement is detected. Thereafter, ultrasound monitoring should be performed at least every 3 days.

[0012] In the present invention, the method for detecting and diagnosing giant panda pregnancy using a B-type ultrasound instrument comprises the following training steps:

[0013] (1) The giant pandas are placed in a familiar and relaxing room and trained by researchers who are familiar with the giant pandas;

[0014] (2) The researchers induced the giant pandas to stand and hold the indoor railings without moving, and gave them food as a reward to establish a conditioned reflex;

[0015] (3) Through long-term, repeated training, giant pandas are made familiar with this posture, which is conducive to abdominal ultrasound examinations.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention uses an ultrasonic diagnostic instrument to perform B-ultrasound monitoring on adult female giant pandas one month after breeding and mating. Before the experiment, the researchers were systematically trained to achieve the effect of successfully cooperating with ultrasonic examinations. A total of 13 pregnancy observations were conducted. Before the examination, the hair on the lower abdomen of the giant panda was cut along the fetal area, and a coupling agent was applied. Then, the fetal position was detected using ultrasound to obtain its image. Experimental verification shows that 15-20 days before the adult female giant panda gave birth, the giant panda fetus can be detected to be 16.85±1.34 days old through B-ultrasound examination. The B-type ultrasonic instrument was successfully used to explore the abdominal wall of the giant panda to diagnose its pregnancy status, and the giant panda's gestation status was understood in real time, which is of great help to the study of issues related to the giant panda's physiology during pregnancy. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1It is a schematic diagram of the structure of the method flow of the present invention;

[0019] Figure 2 This is a comparison chart of the time when the B-type ultrasound instrument of the present invention detects the fetus of a pregnant giant panda. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] See also Figure 1-2 The present invention provides the following technical solution: a method for detecting and diagnosing giant panda pregnancy using a B-type ultrasonic instrument, comprising the following steps:

[0022] (1) Baoding: Through long-term and systematic training of giant pandas by researchers, the experimental giant pandas can make postures that cooperate with B-ultrasound exploration without anesthesia;

[0023] (2) Before performing B-ultrasound examination, the panda's lower abdomen must be trimmed and a coupling agent applied;

[0024] (3) One month after mating, female giant pandas will be monitored by ultrasound to detect the position of the fetus and obtain images. This will be done monthly until uterine enlargement is detected. Thereafter, ultrasound monitoring should be performed at least every 3 days.

[0025] Furthermore, the method of the present invention for detecting and diagnosing giant panda pregnancy using a B-type ultrasound apparatus comprises the following training steps:

[0026] (1) The giant pandas are placed in a familiar and relaxing room and trained by researchers who are familiar with the giant pandas;

[0027] (2) The researchers induced the giant pandas to stand and hold the indoor railings without moving, and gave them food as a reward to establish a conditioned reflex;

[0028] (3) Through long-term, repeated training, giant pandas are made familiar with this posture, which is conducive to abdominal ultrasound examinations.

[0029] Example 1

[0030] The present invention conducts medical management training in the daily feeding of giant pandas. Through the induction of researchers, the giant pandas are made to stand and hold indoor railings to remain motionless, and food is given as a reward to establish conditioned reflexes. Through long-term repeated and accumulative training, the giant pandas are made familiar with this posture that is conducive to abdominal ultrasound examination.

[0031] One month after the giant pandas have bred and mated, B-ultrasound monitoring will begin to detect the fetal position and obtain images. This will be done monthly until the uterus is enlarged. After that, ultrasound monitoring will be done at least every 3 days.

[0032] Before performing B-ultrasound exploration, the panda's lower abdomen needs to be trimmed and coated with coupling agent, and then the probe is used to find a suitable location to observe the image inside the uterus;

[0033] It has been verified through experiments that 15-20 days before an adult female giant panda gives birth, B-ultrasound examination can detect giant panda fetuses (16.85±1.34 days). Figure 2 It can be seen that the method of using B-type ultrasound to diagnose giant panda pregnancy is successful.

[0034] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for detecting the pregnancy status of a giant panda using a B-type ultrasound instrument for non-diagnostic purposes, characterized in that: The following steps are involved: (1) Train giant pandas to maintain a stable standing posture and perform B-ultrasound examinations without anesthesia; (2) Before performing B-ultrasound examination, the panda's lower abdomen must be trimmed and a coupling agent applied; (3) One month after mating, female giant pandas will be monitored by ultrasound to detect the position of the fetus and obtain images. This will be done monthly until uterine enlargement is detected. Thereafter, ultrasound monitoring should be performed at least every 3 days.

2. The method for detecting the pregnancy status of a giant panda using a B-type ultrasonic instrument for non-diagnostic purposes according to claim 1, characterized in that: The steps of training the giant panda to maintain a stable standing posture are as follows: (1) The giant pandas are placed in a familiar and relaxing room and trained by researchers who are familiar with the giant pandas; (2) The researchers induced the giant pandas to stand and hold the indoor railings without moving, and gave them food as a reward to establish a conditioned reflex; (3) Through long-term, repeated training, giant pandas are made familiar with this posture, which is conducive to abdominal ultrasound examinations.

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