A non-contact rotating magnetic field experimental treatment device for inhibiting animal tumors

Abstract

The present invention discloses a non-contact rotating magnetic field experimental device and method for inhibiting animal tumors, and the device includes a magnet unit, a magnet rotating support unit, a power drive unit and an animal plate. The device magnet unit adopts a method of relatively arranging two groups of magnets in an upper and lower space and performing a rotating process, and the magnet is fixed in a rotating support arm that is arranged and fixed up and down. The support arm spacing can be conveniently adjusted according to the magnetic field strength required for the experiment, so as to provide a 0-5T magnetic field strength, and the magnets arranged relatively up and down can make the magnetic field strength distribution of the experimental animal placement area more uniform, which can effectively reduce the experimental error and ensure the credibility of the experimental data. At the same time, the rotating support arm can flexibly remove the fixed cover, and a variety of different magnetic pole loading modes can be set, and then the magnetic pole direction of the magnet can be set according to the magnetic field direction required by the experiment, which solves the problems of uneven magnetic field distribution of the existing rotating magnetic field animal device and the inability to systematically screen effective parameters for tumor inhibition.

Description

Technical Field

[0001] The present invention belongs to the field of mechanics and biomedicine, and relates to a non-contact rotating magnetic field processing device for inhibiting animal tumors and a method for inhibiting animal tumor growth using the device. Specifically, it relates to a magnetic field biomedical experimental device that can be used for tumor physical treatment and research. The device is equipped with two groups of magnets with opposite magnetic poles and a certain distance between them, which move in a circle around the center of a circle. By adjusting the support and controlling the drive system, the key parameters of the rotating magnetic field for inhibiting tumors can be screened, and then the device can be used to inhibit tumor growth. Background Art

[0002] (1) Harms of malignant tumors and progress in treatment

[0003] Cancer is still a serious disease that seriously endangers human life and health. According to a report from the World Health Organization's International Agency for Research on Cancer, there were more than 19.29 million new cases of cancer and more than 9.95 million cancer deaths worldwide in 2021. Analysis found that among people under the age of 70 in 112 countries, cancer is the second leading cause of death. [1] . With the increasing incidence of malignant tumors, there is no standard clinical treatment for malignant tumors at this stage. Surgical treatment, chemotherapy, radiotherapy, targeted therapy, endocrine therapy, etc. are mostly used. In clinical practice, surgical resection combined with radiotherapy and chemotherapy are generally used. However, during the treatment process, patients may experience different degrees of side effects such as hair loss, anorexia, vomiting, fever, infection, anemia, digestive tract and organ toxicity, and skin damage in the radiotherapy area. [2] Some adverse reactions may even last a lifetime and seriously affect the patient's quality of life. Therefore, it is urgent to develop new treatment approaches.

[0004] (2) Physical therapy program - magnetic field

[0005] With the development and deepening of modern precision medicine, as well as the emergence and application of new methods and technologies, some new treatment concepts have been proposed - physical therapy. At present, physical therapy has increasingly penetrated into the field of tumor treatment, mainly including electricity, magnetism, cold, laser, ultrasound, capacitance, radiofrequency ablation, microwave coagulation, whole body hyperthermia, etc. [3,4] , new treatment equipment continues to emerge, such as Gamma Knife, Tom Knife, Hifu Knife, Noli Knife, Proton Knife, Argon-Helium Knife, Ultrasound Focused Knife, etc. Among them, magnetic field has attracted more and more attention due to its non-invasiveness and relatively good safety. With the continuous deepening of magnetic bioeffect research, magnetic field has been gradually applied in clinical practice and has good therapeutic effects, such as pain relief. [5] , anti-inflammatory and detumescent [6] , promote wound healing [7] , promote nerve regeneration [8] , repair bone necrosis [9]and regulate muscle function

[10] wait.

[0006] Since the 1970s, the effect of magnetic fields on tumor cells has been confirmed by numerous experiments at home and abroad. [11,12] For example, in 1999, Pacini et al. found that the use of a 0.2T steady-state magnetic field to treat human breast cancer cells MCF-7 for 48 hours could inhibit cell proliferation.

[13] Luo et al. found that the use of 1T steady-state magnetic field to treat cervical cancer cells HeLa, human colon cancer cells HCT-116, human nasopharyngeal cancer cells CNE-2Z and human breast cancer cells MCF-77 for 2 hours can significantly inhibit cell proliferation and migration ability.

[14] Zhang et al. found that the use of 9T steady-state magnetic field to treat human colon cancer cells HCT-11672h significantly inhibited cell proliferation

[15] Tian et al. found that when three tumor cells (human gastrointestinal stromal tumor cells GIST-T1, human breast cancer cells MCF-7, and human colon cancer cells HCT-116) were exposed to a 0.2-0.5T static magnetic field for 2 days, the proliferation of the three tumor cells was significantly inhibited.

[16] These studies have found that magnetic fields can affect cell proliferation and differentiation to varying degrees. At the same time, with the development of biomagnetic technology in recent years, the application of magnetic fields to treat malignant tumors has attracted more and more attention from scholars.

[0007] Since the differences in magnetic field parameters will lead to differences in the biological effects of magnetic fields, the properties of magnetic fields and their correlation with disease treatment are the focus of current research. Magnetic fields can be divided into two main types based on whether the magnetic field changes over time: steady-state magnetic fields and dynamic magnetic fields. Most current research focuses on the biological effects of steady-state magnetic fields, but even steady-state magnetic fields with fewer parameters can have completely different effects due to differences in magnetic field strength, gradient, processing method, sample type, etc. [17-19] The steady-state magnetic field intensity currently used by researchers is increasing. Tian et al. found that when tumor-bearing mice were treated with a 23T steady-state magnetic field, the tumor volume of the mice in the magnetic exposure group was significantly reduced, but the food intake and weight gain rate were significantly reduced compared with the control group.

[20] Therefore, it is speculated that high-intensity steady-state magnetic fields may cause harm to the human body. At the same time, as the intensity of the magnetic field increases, the volume of the magnetic device also increases rapidly, and the cost is also very expensive, which seriously restricts its application in clinical treatment.

[0008] (3) Application potential of rotating magnetic field

[0009] A dynamic magnetic field is a magnetic field whose direction and intensity change with treatment time. Compared with a steady-state magnetic field, a dynamic magnetic field has more variable parameters, such as magnetic field strength, direction, gradient, and frequency. There are many types of dynamic magnetic fields currently used, among which rotating magnetic fields show good clinical application prospects and can significantly inhibit tumor growth in cells, animals, and humans. Rotating magnetic fields can be generated by electromagnetic fields or rotating permanent magnets, and the frequencies used are generally low, which avoids thermal damage caused by high-frequency magnetic fields. Wang et al. found that rotating magnetic field treatment can significantly inhibit the growth of a variety of tumor cells.

[21] In addition, a study by Han Junqing et al. showed that by using the ZCX rotating magnetic field therapeutic device to treat 32 patients with advanced malignant tumors under different magnetic field strength conditions, the patients did not experience adverse reactions or complications during the treatment, and no local tumor recurrence occurred within 1 year after treatment.

[22] Subsequently, Han Junqing and others used the ZCX rotating magnetic field therapeutic device to treat 137 patients with advanced malignant tumors. The results showed that 20.4% of the patients had significant improvement in their condition, the clinical treatment efficacy was 39.4%, and the survival rate of patients was significantly improved.

[23] Zheng Chunxiu et al. found that the treatment rate of 100 patients with malignant tumors was 54% by using low-frequency rotating magnetic field to treat them.

[24] Sun et al. found that after 6 to 10 weeks of treatment of 13 patients with advanced non-small cell lung cancer with rotating magnetic fields, the 1-year and 2-year survival rates of the patients were 31.7% and 15.9%, respectively. Compared with chemotherapy, rotating magnetic fields had no toxic side effects. Among them, 9 patients (69.2%) showed significant improvement in physical strength during magnetic field exposure treatment. This shows that rotating magnetic fields can be used as an effective and safe new treatment method, and have great potential in the clinical treatment of tumors.

[25] .

[0010] At present, in biomedical research, according to the magnetic field providing method, the existing rotating magnetic field animal experimental devices can be divided into two categories: permanent magnets and electromagnetic coils. The electromagnetic field device will generate heat during the working process, which will cause great interference to the experiment. Therefore, most of the devices still use permanent magnets to provide the magnetic field. The existing rotating magnetic field animal device generally fixes the direction of a single piece or a group of permanent magnets on a rotating disk, and provides a rotating magnetic field with a single direction and uneven magnetic field strength by driving the rotating disk. At the same time, the magnetic field strength can only be changed by adjusting the height difference between the animal feeding disk and the single-sided rotating arm. Therefore, the current rotating magnetic field device has two main disadvantages: (1) It is difficult to change the magnetic poles after the permanent magnet is installed and fixed, which results in a single magnetic field processing direction; (2) The magnetic field distribution is uneven, which will cause errors in the experiment. Therefore, there is an urgent need for a rotating magnetic field device that can provide experimental animals with multiple magnetic field processing methods and a uniform magnetic field distribution.

[0011] References:

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[14] Luo Y,Ji X M,Liu J J,et al.Moderate intensity static magneticfields affect mitotic spindles and increase the antitumor efficacy of 5-FUand Taxol[J].Bioelectrochemistry,2016,109(1):31-40。

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[15] Zhang L,Wang J H,Wang H L,et al.Moderate and strong staticmagnetic fields directly affect EGFR kinase domain orientation to inhibitcancer cell proliferation[J].Oncotarget,2016,7(27):41527-41539。

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[17] Zhang X, Kevin Y, Xu A. Biological effects of static magnetic fields [M]. Springer Singapore, 2017: 81-122.

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[18] Zhang Xin, Kevin Y, Xu An. Biological effects of steady-state magnetic fields[M]. Beijing Science Press, 2018: 50-104.

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[19] Wang HZ, Zhang X. Magnetic fields and reactive oxygen species[J]. Int J Mol Sci, 2017, 18(10): 2175-2195.

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[20] Tian XF, Wang DM, Feng S, et al. Effects of 3.5-23.0T static magnetic fields on mice: A safety study [J]. NeuroImage, 2019, 199(10): 273-280.

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[21] Wang T, Nie Y, Zhao S, et al. Involvement of midkine expression in theinhibitory effects of low-frequency magnetic fields on cancer cells [J]. Bioelectromagnetics, 2011, 32(6): 443-452.

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[22] Han Junqing, Yu Huiming, Wang Yu, et al. Clinical analysis of 32 cases of advanced malignant tumors treated with low-frequency magnetic field rotation [J]. Chinese Journal of Clinical Oncology and Rehabilitation, 2013, 20(1): 59-62.

[0034]

[23] Han Junqing, Liu Qi, Sun Chengtao, et al. Clinical observation of low-frequency rotating magnetic field in the treatment of advanced malignant tumors [J]. Chinese Journal of Oncology, 2013(06):468-471.

[0035]

[24] Zheng Chunxiu, Zhang Shuqing. Clinical observation of low-frequency rotating magnetic field in the treatment of advanced malignant tumors[J]. Chinese Journal of Clinical Oncology and Rehabilitation, 2014, 21(4): 415-417.

[0036]

[25] Sun C, Yu H, Wang Summary of the invention

[0037] In order to overcome the shortcomings of current rotating magnetic field animal devices such as single magnetic field direction and uneven distribution, the present invention intends to provide a non-contact rotating magnetic field processing device that can achieve uniform magnetic field intensity and flexibly control multiple parameters such as magnetic field direction, intensity and exposure frequency.

[0038] The present invention adopts the following technical solution:

[0039] A non-contact rotating magnetic field experimental treatment device for inhibiting animal tumors, characterized by comprising a power drive unit, an animal plate and at least two groups of magnet units;

[0040] One end of the power drive unit is connected to two groups of magnet rotation support units and controls their axial rotation. The two groups of magnet rotation support units are located on the upper and lower sides of the animal disk. Each group of magnet rotation support units includes a magnet support arm bottom plate with holes and grooves on both radial sides and a fixed cover for closing the holes and grooves. The magnet support arm bottom plate and the fixed cover are both stainless steel structures. The two magnets of each group of magnet units are respectively installed in the holes and grooves on the upper and lower sides. A screw with a nut is arranged between the two groups of magnet rotation support units. The two ends of the screw respectively pass through the magnet rotation support units on the upper and lower sides so that the positions of the magnets on the upper and lower sides correspond to each other. The nut controls the distance between the two magnet rotation support units by spiral movement.

[0041] In any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors, when the two upper and lower magnets of each group of magnet units attract each other with opposite polarities, the nut is arranged in a position facing the animal disk.

[0042] In any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors, when the magnets at the two upper and lower positions of each group of magnet units repel each other with the same polarity, the nut is arranged at a position opposite to the animal disk.

[0043] Any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors also includes an upper device support and protection unit, and the device support and protection unit includes a top support plate, an intermediate load-bearing support plate and a bottom load-bearing plate arranged in sequence from top to bottom, and the support plate and the intermediate load-bearing support plate are connected by a top plate support to form a first space for placing an animal plate, a magnet rotation support unit and a magnet unit, and the intermediate load-bearing support plate and the bottom load-bearing plate are connected by a bottom support rod to form a second space for placing a power drive unit.

[0044] In any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors, an animal plate threaded support rod for supporting the animal plate is provided between the animal plate and the middle load-bearing support plate.

[0045] In any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors, the animal disk includes two semi-annular spliced ​​disks, which are spliced ​​together by a riveted structure at the interface, and each semi-annular spliced ​​disk is provided with a disassembly cover that is sealed and matched therewith.

[0046] In any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors, the power drive unit includes a drive motor, a spindle screw, a planetary gearbox and a coupling; one end of the spindle screw 5 is axially connected to the magnet rotation support unit, and the other end is connected to the planetary gearbox through a coupling, and the drive motor is transmission connected to the planetary gearbox.

[0047] In any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors, the top of the top support plate includes a bearing, and one end of the main shaft screw extends upward and is connected to the bearing.

[0048] Any of the above-mentioned non-contact animal tumor inhibition rotating magnetic field experimental treatment devices also includes a waterproof side plate, which is installed on the edge of the bottom load-bearing plate.

[0049] In any of the above-mentioned non-contact rotating magnetic field experimental treatment devices for inhibiting animal tumors, the power drive unit also includes a driver, a DC power supply, a drive electronic transformer, a drive speed regulator, and a drive motor line control line. The driver is connected to the drive motor through the drive motor line to achieve the operation of the drive motor, the DC power supply connects the speed regulator to the driver through the drive encoder line and the driver X4 plug control line, the electronic transformer connects the drive and the power supply, and the speed regulator and the driver and other components jointly drive the spindle to rotate, which can ensure that the magnet support arm rotates at the set frequency. The control unit includes a human-machine operation interface, and the human-machine operation interface includes a frequency setting area and a time setting area.

[0050] In any of the above-mentioned non-contact animal tumor inhibition rotating magnetic field experimental processing devices, the processing steps are:

[0051] ⑦Process the magnet according to the experimental requirements;

[0052] ⑧ Adjust the magnetic pole direction to set the magnetic field direction;

[0053] ⑨ Adjust the distance between the rotating arms to set the magnetic field strength;

[0054] ⑩ Place experimental animals;

[0055] Set the speed and process time;

[0056] Expose to magnetic field and collect data.

[0057] The beneficial effects of the present invention are:

[0058] 1. The non-contact rotating magnetic field animal tumor treatment device of the present invention can change the magnetic field loading intensity by adjusting the distance between the magnetic poles of the rotating arm, and produce different degrees of treatment effects on animal tumors within the magnetic field intensity range of 0 to 5T.

[0059] 2. The non-contact rotating magnetic field animal tumor treatment device of the present invention can ensure that the rotating magnetic field treatment frequency is accurately adjustable within the range of 0 to 1500 rpm through a drive control system.

[0060] 3. The non-contact rotating magnetic field animal tumor treatment device of the present invention adopts a vertical relative spatial arrangement mode of the magnets to ensure a more uniform magnetic field. At the same time, the magnetic field loading direction can be changed by changing the direction of the magnetic poles of the magnets in the fixed support arm to meet the needs of different experiments.

[0061] This non-contact rotating magnetic field experimental device for inhibiting animal tumors effectively makes up for the single parameter condition of the traditional steady-state magnetic field that cannot be adjusted in experimental research, and avoids the defects of excessive magnetic field intensity, large device size, and biosafety of the steady-state magnetic field, effectively improving research efficiency and making the operation safe and simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0062] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0063] Figure 1 A three-dimensional diagram of the non-contact rotating magnetic field treatment device for inhibiting animal tumors according to the present invention;

[0064] Figure 2 It is a partial stereoscopic diagram of the non-contact rotating magnetic field treatment device for inhibiting animal tumors of the present invention;

[0065] Figure 3It is a plan view of the non-contact rotating magnetic field treatment device for inhibiting animal tumors of the present invention;

[0066] Figure 4 for Figure 1 Exploded view of the experimental animal dish;

[0067] Figure 5 for Figure 1 A perspective view of the experimental animal dish in the middle;

[0068] Figure 6 for Figure 1 An exploded view of the two sets of magnet rotation support units;

[0069] In the figure, 1 is the top support plate, 2 is the top plate support, 3 is the bottom plate of the magnet support arm, 4 is the fixed cover, 5 is the main shaft screw, 6 is the screw, 7 is the animal disk, 8 is the animal disk threaded support rod, 9 is the coupling, 10 is the intermediate load-bearing support plate, 11 is the bottom support rod, 12 is the bottom load-bearing plate, 13 is the waterproof side plate, 14 is the planetary gear box, 15 is the drive motor, 16 is the driver, 17 is the drive electronic transformer, 18 is the drive speed regulator, 19 is the support connection fixing nut, 20 is the bearing, 21 is the T-nut, 22 is the removable cover of the animal disk, 23 is the fixing nut, 30 is two sets of magnet rotation support units, and 31 is the hole groove. DETAILED DESCRIPTION

[0070] In order to better understand the above technical solution of the present invention, it is further described in detail below with reference to the accompanying drawings and embodiments.

[0071] like Figures 1 to 3 As shown, a non-contact rotating magnetic field experimental device for inhibiting animal tumors provided by the present invention includes a magnet unit, a magnet rotation support unit, a power unit, an animal plate, a device support and protection unit, and a drive control unit. The above magnets include but are not limited to permanent magnets.

[0072] The present invention also includes an upper device support and protection unit, which includes a top support plate 1, an intermediate load-bearing support plate 10 and a bottom load-bearing plate 12 arranged in sequence from top to bottom. The support plate 1 and the intermediate load-bearing support plate 10 are connected by a top plate support column 2 to form a first space for placing an animal disk 7, a magnet rotation support unit 30 and a magnet unit. The intermediate load-bearing support plate 10 and the bottom load-bearing plate 12 are connected by a bottom support rod 11 to form a second space for placing a power drive unit.

[0073] One end of the power driving unit is connected to two sets of magnet rotation supporting units 30 through a T-shaped nut 21 and controls the axial rotation thereof. The two sets of magnet rotation supporting units are located at the upper and lower sides of the animal disk 7 .

[0074] like Figure 6 As shown, each group of magnet rotation support units 30 includes a magnet support arm base plate 3 with holes 31 on both radial sides and a fixed cover 4 for closing the holes 31. The magnet support arm base plate 3 and the fixed cover 4 are both stainless steel structures. The magnet support arm base plate 3 and the fixed cover 4 are fixedly connected by screws. The two magnets of each group of magnet units are respectively installed in the holes 31 on the upper and lower sides. The structure of the magnet is a permanent magnet. The magnet support arm base plate 3 and the fixed cover 4 are fixedly connected by screws to fix the installed magnets. A screw rod 6 with a nut is arranged between the two groups of magnet rotation support units 30. The nut is divided into a fixing nut 23 and a supporting connection fixing nut 19, and is respectively located on the upper and lower sides of the screw rod 6. The two ends of the screw rod respectively pass through the magnet rotation support units on the upper and lower sides so that the magnet positions on the upper and lower sides correspond to each other. The nut controls the distance between the two magnet rotation support units by spiral movement.

[0075] When the magnets at the two upper and lower positions of each group of magnet units attract each other with opposite poles, such as upper N-lower S and upper S-lower N, due to the attraction of the opposite poles of the magnets, the nut is divided into a supporting connection fixing nut 19 and a fixing nut 23 to rotate and drive the magnet rotation support unit to move in the opposite direction of the animal disk 7, thereby adjusting the distance between the two magnet rotation support units and further adjusting the size of the magnetic field.

[0076] When the magnets at the two upper and lower positions of each group of magnet units repel each other with the same polarity, such as the upper NN and SS, the nut is set at a position in the opposite direction of the animal plate 7. Since magnets of the same polarity repel each other, the nuts are divided into the supporting connection fixing nut 19 and the fixing nut 23 to rotate and drive the magnet rotation support unit to move in the direction of the animal plate 7, thereby adjusting the distance between the two magnet rotation support units and further adjusting the size of the magnetic field.

[0077] like Figure 4 and Figure 5 As shown, an animal tray threaded support rod 8 for supporting the animal tray 7 is provided between the above-mentioned animal tray 7 and the intermediate load-bearing support plate 10. The animal tray 7 is composed of two semi-annular spliced ​​discs, which are spliced ​​by a riveted structure at the interface. Each semi-annular spliced ​​disc is provided with a removable animal tray cover 22 that is sealed and matched with it, so as to facilitate disassembly and disinfection during the experiment. The outer wall and the inner wall of the animal tray are equidistantly provided with ventilation holes to facilitate the animal to breathe and drink water. The horizontal top surface of the animal disc is provided with a circular fan-shaped opening, and a top cover of corresponding size is provided to facilitate the removal and placement of experimental animals.

[0078] The above-mentioned power drive unit includes a drive motor 15, a spindle screw 5, a planetary gearbox 14 and a coupling 9. In addition, a driver 16, a drive electronic transformer 17 and a drive speed regulator 18 are all installed and fixed on the surface of the bottom bearing plate 12. One end of the spindle screw 5 is axially connected to the magnet rotation support unit, and the other end is connected to the planetary gearbox 14 through a coupling 9. The drive motor 15 is connected to the planetary gearbox 14 in a transmission manner. The top of the top support plate 1 includes a bearing 20, and one end of the spindle screw 5 extends upward and is connected to the bearing 20. The drive electronic transformer 17 connects the power supply to the driver 16 to provide driving force for the drive motor 15 and the planetary gearbox 14, and the planetary gearbox 14 effectively ensures that the rotating arm and the spindle can operate accurately according to the set frequency, and the drive speed regulator 18 controls the rotation frequency through the driver 16. The bottom plate waterproof side plate 13 is arranged around the bottom bearing plate 12 to prevent the damage of the device to the ground water.

[0079] The operation process of the non-contact rotating magnetic field experimental device for inhibiting animal tumors of the present invention is as follows:

[0080] 1. Prepare magnets and magnetic conductive materials of special shapes required for the experiment.

[0081] 2. Install and fix the prepared magnets and magnetic conductive materials. The installation and fixing method is not fixed, and the magnets and magnetic conductive materials can be installed in a rotating support unit with adjustable spacing, but are not limited to the installation method.

[0082] 3. According to the size of the experimental animal and the required magnetic field direction and strength, adjust the magnet rotating support unit of the non-contact animal tumor inhibition rotating magnetic field experimental device of the present invention, and replace the internal magnet by opening the fixed cover 4 to ensure that the magnetic field direction has been replaced. By adjusting the position of the nut on the top of the middle support connecting screw 6 of the upper and lower magnet rotating support units 30, change the distance between the upper and lower magnet rotating support units 30 and adjust to the required magnetic field strength position.

[0083] 4. Adjust the threaded support rod 8 of the animal tray to place the animal tray 7 in the magnetic field treatment position, open the detachable cover 22 of the animal tray, put the experimental animals in, and place animal drinking bottles at intervals between the ventilation and drinking holes of the animal tray 7.

[0084] 5. Make the magnet or magnetic material rotate around the main shaft screw perpendicular to the magnet rotation support unit 30. The rotation can be clockwise or counterclockwise. Check the connection status of the drive system, make sure the connection is correct, and turn on the main power. Turn on the driver 16, connect the DC power supply to the drive speed regulator 18, turn on the speed regulator, set the rotation speed, and gradually increase the speed to the set processing frequency.

[0085] 6. Set the processing time and record the processing data.

[0086] 7. After the processing is completed, use the drive speed regulator to gradually reduce the speed to 0rpm, turn off the speed regulator and drive, and turn off the main power supply.

[0087] 8. Open the top cover of the animal dish, take out the experimental animals, and collect and record the experimental data.

[0088] 9. Data collection and analysis.

[0089] The magnetic field strength range that can be generated by the magnet rotation support unit 30 with built-in magnets is 0-5T by adjusting the upper and lower spacings, and the rotation frequency that can be achieved by the permanent magnets is 0-1500rpm through the driving control system.

[0090] The annular space of the experimental animal disk, i.e. the distance between the inner and outer diameters, can cover the uniform magnetic field area of ​​the upper and lower magnetic fields, ensuring that the experimental animals can be uniformly treated with a rotating magnetic field of substantially the same intensity within the set area.

[0091] The operation process of the non-contact rotating magnetic field experimental device for inhibiting animal tumors of the present invention is as follows:

[0092] 3. Prepare magnets and magnetic conductive materials of special shapes required for the experiment.

[0093] 4. Install and fix the prepared magnets and magnetic conductive materials. The installation and fixing method is not fixed, and the magnets and magnetic conductive materials can be installed in a rotating support unit with adjustable spacing, but are not limited to the installation method.

[0094] 3. According to the size of the experimental animal and the required magnetic field direction and strength, adjust the magnet rotating support unit assembly of the non-contact animal tumor inhibition rotating magnetic field experimental device of the present invention, replace the internal magnet by opening the detachable fixed cover of the rotating support arm, and make sure that it has been replaced to the required magnetic field direction; change the distance between the upper and lower rotating support arms by adjusting the position of the nut on the top of the middle support connecting screw of the upper and lower rotating support arms, and adjust to the required magnetic field strength position.

[0095] 4. Adjust the threaded support rod of the animal tray to place the animal tray in the magnetic field treatment position, open the top cover of the animal tray, put the experimental animals in, and place animal drinking bottles at intervals between the ventilation and drinking holes of the animal tray.

[0096] 5. Make the magnet or magnetic material rotate around the main axis perpendicular to the rotating support arm. The rotation can be clockwise or counterclockwise. Check the connection status of the drive system, make sure the connection is correct, and turn on the main power. Turn on the driver, connect the DC power supply to the drive speed regulator, turn on the speed regulator, set the rotation speed, and gradually increase the speed to the set processing frequency.

[0097] 6. Set the processing time and record the processing data.

[0098] 7. After the processing is completed, use the drive speed regulator to gradually reduce the speed to 0rpm, turn off the speed regulator and drive, and turn off the main power supply.

[0099] 8. Open the top cover of the animal dish, take out the experimental animals, and collect and record the experimental data.

[0100] 9. Data collection and analysis.

[0101] Embodiment 1:

[0102] The non-contact rotating magnetic field device for inhibiting animal tumors provided by the present invention is used to inhibit the growth of animal tumor tissues by screening and determining the magnetic field with specific parameters. The specific implementation method is exemplified as follows:

[0103] Step 1: Take the triple-negative breast cancer cells MDA-MB-231 that are in good growth state and in the logarithmic growth phase and inject them into the second pair of breast pads on the left side of BABL / c female nude mice. After the tumor cells are inoculated, the tumor volume reaches 200mm 3 A total of 14 nude mice were treated with rotating magnetic field at the same time.

[0104] Step 2: Prepare the matching Figure 6 The cubic permanent magnet of the blind hole size shown in the figure is placed in the hole slot 31 of the bottom plate 3 of the magnet support arm, and the rotating arm is assembled into the device. The installation and fixing method is not fixed. The permanent magnet spacing is adjusted according to the experimental animal, and the position of the animal plate 7 is adjusted. Tumor-bearing nude mice are placed, and the tumor-bearing part is exposed to the device provided by the present invention. The specific position is on the operating platform between the two magnets, and the magnetic field strength at the tumor position of the nude mouse is 0.4T.

[0105] Step 3: Check the connection status of the drive system, turn on the driver 16, connect the DC power supply and the drive speed regulator 18, turn on the speed regulator, set the rotation speed to 100 rpm, and gradually increase the speed to the set treatment frequency. The tumor of each tumor-bearing mouse was treated with a magnetic field for 3 hours every day, and the fixed treatment time was 9:00-12:00 every day. The tumor-bearing mice in the control group were not treated with the magnetic field provided by the present invention.

[0106] Step 4: Observe the status of each group of nude mice every day, record the weight and tumor volume of nude mice, and after 35 days of continuous treatment with the rotating magnetic field, the results showed that compared with the control group, the net tumor volume of the tumor-bearing nude mice in the experimental group decreased by 59.93% and the net tumor weight decreased by 80.21% after 35 days of continuous treatment with the rotating magnetic field device. The experimental results show that the magnetic field with specific parameters provided by the non-contact animal tumor inhibition rotating magnetic field device can effectively inhibit the growth of nude mouse tumors.

[0107] The above briefly describes the embodiments of the present invention, but the present invention is not limited to the above specific implementations. Researchers in the technical field should recognize that the above embodiments are only used to illustrate the operating technical solutions of the present invention, and are not used as limitations of the present invention. Any changes and modifications made to the above embodiments based on the essential spirit of the present invention will fall within the scope of protection of the claims of the present invention.

Claims

1. A non-contact rotating magnetic field experimental treatment device for inhibiting animal tumors, characterized in that: It comprises a power drive unit, an animal plate (7) and at least two groups of magnet units; One end of the power drive unit is connected to two groups of magnet rotation support units (30) and controls their axial rotation. The two groups of magnet rotation support units are located on the upper and lower sides of the animal disk (7). Each group of magnet rotation support units (30) comprises a magnet support arm bottom plate (3) with holes (31) arranged on both radial sides and a fixed cover (4) for closing the holes (31). The two magnets of each group of magnet units are respectively installed in the holes (31) on the upper and lower sides. A screw rod (6) with a nut is arranged between the two groups of magnet rotation support units (30). The two ends of the screw rod respectively pass through the magnet rotation support units on the upper and lower sides so that the positions of the magnets on the upper and lower sides correspond to each other. The nut controls the distance between the two magnet rotation support units by spiral movement. When the two upper and lower magnets of each group of magnet units attract each other with opposite polarities, the nut is arranged in a position facing the animal plate (7); driving the magnet rotation support unit (30) to move in the opposite direction of the animal plate (7); When the two upper and lower magnets of each group of magnet units repel each other with the same polarity, the nut is arranged in a position opposite to the animal plate (7), driving the magnet rotation support unit (30) to move in the direction of the animal plate (7); It also includes a device support and protection unit, the device support and protection unit includes a top support plate (1), an intermediate load-bearing support plate (10) and a bottom load-bearing plate (12) arranged in sequence from top to bottom, the top support plate (1) and the intermediate load-bearing support plate (10) are connected via a top plate support column (2) to form a first space for placing an animal plate (7), a magnet rotation support unit (30) and a magnet unit, and the intermediate load-bearing support plate (10) and the bottom load-bearing plate (12) are connected via a bottom support rod (11) to form a second space for placing a power drive unit; An animal tray threaded support rod (8) for supporting the animal tray (7) is provided between the animal tray (7) and the intermediate load-bearing support plate (10).

2. The non-contact animal tumor inhibition rotating magnetic field experimental treatment device according to claim 1 is characterized in that: The animal plate (7) comprises two semi-annular spliced ​​circular plates, which are spliced ​​together via a riveted structure at the interface, and each semi-annular spliced ​​circular plate is provided with a detachable animal plate cover (22) which is sealed and matched therewith.

3. The non-contact animal tumor inhibition rotating magnetic field experimental treatment device according to claim 1 is characterized in that: The power drive unit comprises a drive motor (15), a main shaft screw (5), a planetary gear box (14) and a coupling (9); one end of the main shaft screw (5) is axially connected to the magnet rotation support unit, and the other end is connected to the planetary gear box (14) via the coupling (9); the drive motor (15) is transmission-connected to the planetary gear box (14).

4. The non-contact animal tumor inhibition rotating magnetic field experimental treatment device according to claim 3 is characterized in that: The top of the top support plate (1) includes a bearing (20), and one end of the main shaft screw (5) extends upward and is connected to the bearing (20).

5. The non-contact rotating magnetic field experimental treatment device for inhibiting animal tumors according to claim 1 is characterized in that: It also includes a waterproof side plate (13), which is installed on the edge of the bottom load-bearing plate (12).

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