Long-term time-series sampling and preservation device for benthic organisms in deep-sea mining areas

Abstract

The present invention relates to a deep-sea long-period biological sampling technology, and aims to provide a long-period sequential sampling and preservation device for benthic organisms in deep-sea mining areas. The device includes a sample chamber module, a sampling module, a preservation liquid injection module, and a circuit switching module; wherein the sample chamber module includes a sample chamber composed of a chamber shell and a chamber end cover, as well as a reserved space and several sub-sample chambers provided inside the sample chamber; the sampling module includes a motor barrel, a sampling switching motor, and a sampling tube; the preservation liquid injection module includes a motor barrel, a screw motor, an injection piston, and a preservation liquid storage barrel; the circuit switching module includes a motor barrel, a reversing valve switching motor, a reversing valve, and a multi-way valve. The present invention can meet the continuous and sequential sampling requirements for long-term monitoring of deep-sea mining areas; it can realize multi-chamber sampling and preservation liquid injection, and has a simple structure and is easy to implement.

Description

Technical Field

[0001] The present invention relates to a deep-sea long-period biological sampling technology, and in particular to a long-period time-series sampling and preservation device for benthic organisms in deep-sea mining areas. Background Art

[0002] With the increasing frequency of deep-sea mining activities, the plumes generated by mining are significantly impacting benthic ecosystems through factors such as increased suspended particulate matter, light attenuation, reduced oxygen availability, and increased levels of toxic substances like trace metals. These impacts are highly detrimental to important functions and metabolism of benthic organisms, including feeding, respiration, growth, and even increased mortality.

[0003] Monitoring and assessing the potential environmental impacts of deep-sea mining activities is crucial, but current technology makes this difficult to achieve. Sampling deep-sea organisms is a crucial tool for assessing the impacts of deep-sea mining activities. Current deep-sea benthic sampling techniques are mostly short-term, one-time operations. These typically involve deploying a sampler on the seafloor for several hours, collecting samples, and then returning them to the surface. It's important to understand that deep-sea mining is a long-term, continuous process, and this transient sampling approach is insufficient to assess the long-term effects and potential impacts of deep-sea mining.

[0004] Furthermore, most current benthic sampling techniques fail to consider sample preservation. This is because these samplers are designed for short-term sampling cycles and do not require specialized preservation devices. However, to achieve long-term sampling and sample recovery for research, appropriate preservation measures (such as injection of biomass-preserving fixatives such as alcohol, formalin, or RNAlater) are necessary, but these issues are clearly not considered in the design of existing samplers. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide a long-period time-series sampling and preservation device for benthic organisms in deep-sea mining areas.

[0006] To solve the technical problem, the solution of the present invention is:

[0007] Provided is a long-period time-series sampling and preservation device for benthic organisms in deep-sea mining areas, comprising a sample chamber module, a sampling module, a preservation fluid injection module, and a circuit switching module; wherein the sample chamber module comprises a sample chamber consisting of a chamber shell and a chamber end cover, as well as a reserved space and several sub-sample chambers arranged inside the sample chamber; the sampling module comprises a motor barrel, a sampling switching motor, and a sampling tube; the preservation fluid injection module comprises a motor barrel, a screw motor, an injection piston, and a preservation fluid storage barrel; the circuit switching module comprises a motor barrel, a reversing valve switching motor, a reversing valve, and a multi-way valve; wherein,

[0008] In the sample chamber, the internal space of the sample chamber is evenly divided along the circumference and used as a reserved space and a placeholder for each sub-sample chamber. The sampling tube is longitudinally arranged in the center of the sample chamber, and each sub-sample chamber is fitted with the surface of the sampling tube through the arc surface of the inner wall; the bottom of the sampling tube is an open end, and its top closed end is connected to the output shaft of the sampling switching motor through a coupling; the sampling switching motor is fixed to the upper surface of the sample chamber and is used to drive the sampling tube to rotate axially; side wall holes are provided on the side walls of the sampling tube, and corresponding side wall holes are provided on the inner wall arc surface of each sub-sample chamber for mutual communication; each group of sub-sample chambers consists of a shell and an end cover, and the end cover is provided with a sample chamber inlet connector and a sample chamber outlet connector;

[0009] A lead screw motor and a lead screw are provided inside the motor cylinder of the preservative liquid injection module, and an injection piston is provided inside the preservative liquid storage cylinder. The motor cylinder and the preservative liquid storage cylinder are connected end to end, and the lead screw motor is nested on the lead screw. The piston rod of the injection piston is connected to the lead screw, and the lead screw motor can drive the injection piston to move within the preservative liquid storage cylinder. A preservative liquid outlet connector and a preservative liquid inlet connector are provided at the end of the preservative liquid storage cylinder on the side opposite to the piston rod.

[0010] A multi-way valve is installed at the open end of the motor cylinder of the loop switching module; a multi-way valve inlet connector is provided at the center of the end face of the multi-way valve, and several multi-way valve outlet connectors are provided on its side; a reversing valve is coaxially nested inside the multi-way valve, and an axial bottom inlet and a lateral outlet that are interconnected are provided inside the reversing valve, and the bottom inlet is connected to the multi-way valve inlet connector; a reversing valve switching motor is provided inside the motor cylinder, and its output shaft is fixedly connected to the axial center of the reversing valve through a coupling; the reversing valve switching motor can drive the reversing valve to rotate so that its lateral outlets are respectively connected to each multi-way valve outlet connector, thereby realizing multi-way output switching of the multi-way valve; the multi-way valve inlet connector is connected to the preservation liquid outlet connector through a pipeline, and each multi-way valve outlet connector is connected to the sample chamber inlet connector of each sub-sample chamber through a pipeline.

[0011] As a preferred solution of the present invention, the shell of the sub-sample cabin is a hollow structure with an open end, and a sealing ring and an end cover are provided at the open end, and are fixedly installed by screws; the cabin body shell of the sample cabin is a hollow structure with an open end, and a sealing ring and a cabin body end cover are provided at the open end, and are fixedly installed by screws; a number of through holes are provided on the cabin body end cover for exposing the sample cabin inlet connector and the sample cabin outlet connector on the end cover of each sub-sample cabin.

[0012] As a preferred solution of the present invention, the open end of the sampling tube is provided with a bell-shaped sampler.

[0013] As a preferred solution of the present invention, in the sampling module, the sampling switching motor is fixedly installed in the motor cylinder, and the motor cylinder is fixedly installed on the upper surface of the sample chamber through the connecting cylinder.

[0014] As a preferred solution of the present invention, the sample chamber, the reserved space and the sub-sample chamber have any one of the following structural features:

[0015] (1) The transverse cross-section of the sample chamber is circular; the transverse cross-sections of the reserved spaces and each sub-sample chamber are fan-shaped;

[0016] (2) The transverse cross-section of the sample chamber is a regular polygon; the inner edges of the transverse cross-sections of the reserved space and each sub-sample chamber are the same arc shape, and their outer edges are adapted to the shape of the sample chamber.

[0017] As a preferred solution of the present invention, the motor cylinder and the preservation liquid storage cylinder in the circuit switching module are installed in parallel in a fixed frame, and the fixed frame is installed on the cabin shell of the sample cabin.

[0018] As a preferred solution of the present invention, the side wall holes of the sampling tube and the side wall holes of each sub-sample chamber are long rectangular or waist-shaped openings along the axial direction of the sampling tube, and the size and arrangement height of each opening are the same; a sealing ring is installed in the side wall hole of the sampling tube or the side wall hole of the sub-sample chamber, and the side wall of the sampling tube and the arc surface of the inner wall of each sub-sample chamber are fitted to maintain a gap, so that the sampling tube can rotate around the axis while sealing each sub-sample chamber.

[0019] As a preferred solution of the present invention, when the preservative liquid storage cylinder is in a state of maximum volume, the end of the screw at the opposite end of the injection piston is close to the end of the motor cylinder.

[0020] As a preferred solution of the present invention, the multi-way valve is fixed to the open end of the motor cylinder through a mounting base with a central opening, one end of the reversing valve switching motor is fixed to the mounting base and the other end is fixed to the motor cylinder, and its output shaft is fixedly connected to the protrusion in the center of the reversing valve through a coupling located in the central opening of the mounting base.

[0021] As a preferred solution of the present invention, the sampling switching motor, the lead screw motor and the reversing valve switching motor are all oil-filled motors.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. Existing benthic sampling technologies are short-term, one-time applications. This transient sampling model is insufficient to explore the long-term effects and potential impacts of deep-sea mining. The proposed long-term benthic sampling and preservation technology for deep-sea mining areas can meet the continuous, time-series sampling requirements for long-term monitoring of deep-sea mining areas.

[0024] 2. Existing technologies only consider sampling or preservation in a single chamber, requiring one driver for sampling and another for injecting the preservation solution. The present invention uses three motors as drivers, one for switching sub-sampling chambers, injecting the preservation solution, and distributing the preservation solution flow path. This allows for multi-chamber sampling and preservation solution injection, resulting in a simple structure and ease of implementation.

[0025] 3. A hollow sampling tube is used, and organisms enter from the end and enter the sub-sample chamber through the opening of the side wall. The sub-sampling chamber can be switched by simply driving the sampling motor to rotate it to a certain angle.

[0026] 4. The device system structure of the present invention is simple and easy to implement. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a structural schematic diagram of the device of the present invention;

[0028] Figure 2 yes Figure 1 Bottom view of the device in the middle;

[0029] Figure 3 This is a schematic diagram of the structure of the sample cabin after the cabin end cover is removed;

[0030] Figure 4 It is a structural diagram of a single sub-sample chamber module;

[0031] Figure 5 is a cross-sectional view of the preservation solution injection module;

[0032] Figure 6 This is a cross-sectional view of the circuit switching module.

[0033] Reference numerals in the figure: 1 sample chamber module; 2 sampling module; 3 preservation solution injection module; 4 loop switching module; 1-1 chamber shell; 1-2 chamber end cover; 1-3 sample chamber inlet connector; 1-4 sample chamber outlet connector; 1-5 end cover; 1-6 sub-sample chamber; 1-6-1 first sub-sample chamber; 1-6-2 second sub-sample chamber; 1-6-3 third sub-sample chamber; 1-7 opening end; 1-8 side wall hole; 2-1 sampling switching motor; 2-2 connecting tube; 2-3 sampling tube ; 2-4 Side wall hole; 3-1 Motor cylinder; 3-2 Fixed rod; 3-3 Preservative liquid storage cylinder; 3-4 Fixed frame; 3-5 Preservative liquid inlet connector; 3-6 Preservative liquid outlet connector; 3-7 Multi-way valve inlet connector; 3-8 Multi-way valve outlet connector; 3-9 Screw motor; 3-10 Screw; 3-11 Injection piston; 3-12 Reversing valve switching motor; 3-13 Reversing valve; 3-14 Motor cylinder; 3-15 Bottom inlet; 3-16 Side outlet; 3-17 Multi-way valve. DETAILED DESCRIPTION

[0034] The following examples are provided to enable those skilled in the art to more fully understand the present invention, but are not intended to limit the present invention in any way.

[0035] The serial numbers assigned to the components in this application, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings). In the description of this application, it should be understood that the orientation or position relationship indicated by the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc. are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to this application.

[0036] In this application, unless otherwise expressly specified or limited, when a first feature is "above" or "below" a second feature, it may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Furthermore, when a first feature is "above," "above," or "above" a second feature, it may mean that the first feature is directly above or diagonally above the second feature, or simply means that the first feature is at a higher level than the second feature. When a first feature is "below," "below," or "below" a second feature, it may mean that the first feature is directly below or diagonally below the second feature, or simply means that the first feature is at a lower level than the second feature.

[0037] As shown in the figure, the long-period time-series sampling and preservation device for benthic organisms in deep-sea mining areas of the present invention includes a sample chamber module 1, a sampling module 2, a preservation liquid injection module 3 and a circuit switching module 4; wherein, the sample chamber module 1 includes a sample chamber composed of a chamber shell 1-1 and a chamber end cover 1-2, as well as a reserved space and several sub-sample chambers 1-6 provided inside the sample chamber; the sampling module 2 includes a motor cylinder, a sampling switching motor 2-1 and a sampling tube 2-3; the preservation liquid injection module 3 includes a motor cylinder 3-1, a screw motor 3-9, an injection piston 3-11 and a preservation liquid storage cylinder 3-3; the circuit switching module 4 includes a motor cylinder 3-14, a reversing valve switching motor 3-12, a reversing valve 3-13 and a multi-way valve 3-17.

[0038] The shell of the sub-sample cabin 1-6 is a hollow structure with an open end, and a sealing ring and an end cover 1-5 are provided at its open end 1-7, and fixedly installed by screws; the cabin body shell 1-1 of the sample cabin is a hollow structure with an open end, and a sealing ring and a cabin end cover 1-2 are provided at its open end, and fixedly installed by screws; a number of through holes are provided on the cabin end cover 1-2 for exposing the sample cabin inlet joint 1-3 and the sample cabin outlet joint 1-4 on each sub-sample cabin end cover 1-5.

[0039] In the sample chamber, the internal space is evenly divided circumferentially to accommodate empty space and the sub-sample chambers 1-6. A sampling tube 2-3 is longitudinally inserted through the center of the chamber, with each sub-sample chamber 1-6 contacting the surface of the sampling tube 2-3 via the curved inner surface. The bottom of the sampling tube 2-3 is open, optionally equipped with a bell-shaped sampler. The top, closed end of the sampling tube 2-3 is connected to the output shaft of a sampling switching motor 2-1 via a coupling. The sampling switching motor 2-1 is fixed to the upper surface of the sample chamber and is used to drive the sampling tube 2-3 in axial rotation. The sidewall of the sampling tube 2-3 is provided with a sidewall hole 2-4, and each sub-sample chamber 1-6 has a corresponding sidewall hole 1-8 on its curved inner surface to achieve interconnection.

[0040] The sample chamber, the reserved space, and the sub-sample chamber may optionally have the following structural features: the transverse cross-section of the sample chamber is circular; the transverse cross-section of the reserved space and each sub-sample chamber 1-6 is fan-shaped; or the transverse cross-section of the sample chamber is a regular polygon; the inner edges of the transverse cross-sections of the reserved space and each sub-sample chamber 1-6 are the same arc shape, and their outer edges are adapted to the shape of the sample chamber. The side wall holes 2-4 of the sampling tube 2-3 and the side wall holes 1-8 of each sub-sample chamber are long rectangular or waist-shaped openings along the axis of the sampling tube 2-3, and the openings are of the same size and arrangement height. A sealing ring is installed in the side wall hole 2-4 or the side wall hole 1-8, and the side wall of the sampling tube 2-3 fits with the inner wall arc surface of each sub-sample chamber 1-6 to maintain a gap, so that the sampling tube 2-3 can rotate around the axis while sealing each sub-sample chamber 1-6.

[0041] A screw motor 3-9 and a screw 3-10 are located within the motor barrel of the preservative liquid injection module 3, and an injection piston 3-11 is located within the preservative liquid storage barrel 3-3. The motor barrel and the preservative liquid storage barrel 3-3 are connected end to end, and the screw motor 3-9 is nested on the screw 3-10. The piston rod of the injection piston 3-11 is connected to the screw 3-10, and the screw motor 3-9 can drive the injection piston 3-11 to move within the preservative liquid storage barrel 3-3. At the end of the preservative liquid storage barrel 3-3 on the opposite side of the piston rod, a preservative liquid outlet connector 3-6 and a preservative liquid inlet connector 3-5 are located. When the preservative liquid storage barrel 3-3 is at its maximum volume, the injection piston 3-11 is at the uppermost end, and the end of the screw 3-10 on the opposite end is close to the end of the motor barrel.

[0042] A multi-way valve 3-17 is installed at the open end of the motor barrel 3-14 of the circuit switching module 4; a multi-way valve inlet connector 3-7 is provided at the center of the end face of the multi-way valve 3-17, and several multi-way valve outlet connectors 3-8 are provided on its side. A reversing valve 3-13 is coaxially nested inside the multi-way valve 3-7. The reversing valve 3-13 is provided with an axial bottom inlet 3-15 and a lateral outlet 3-16 that are interconnected, and the bottom inlet 3-15 is connected to the multi-way valve inlet connector 3-7. A reversing valve switching motor 3-12 is installed inside the motor barrel 3-14, and its output shaft is fixedly connected to the axial center of the reversing valve 3-13 via a coupling. The reversing valve switching motor 3-12 can drive the reversing valve 3-13 to rotate, so that its lateral outlets 3-16 are connected to each multi-way valve outlet connector 3-8 respectively, realizing multi-way output switching of the multi-way valve. The multi-way valve inlet connector 3-7 is connected to the preservation solution outlet connector 3-6 through a pipeline, and each multi-way valve outlet connector 3-8 is connected to the sample chamber inlet connector 1-3 of each sub-sample chamber 1-6 through a pipeline.

[0043] In sampling module 2, the sampling switching motor 2-1 is fixedly mounted within the motor barrel, which is then fixed to the upper surface of the sample chamber via a connecting barrel 2-2. The motor barrel 3-14 in the loop switching module 4 is mounted alongside the preservative fluid storage barrel 3-3 within a fixed frame 3-4, which is mounted on the sample chamber's housing 1-1. The multi-way valve 3-17 is secured to the open end of the motor barrel 3-14 via a mounting base with a central opening. The reversing valve switching motor 3-12 is secured to the mounting base at one end and to the motor barrel 3-14 at the other. Its output shaft is fixedly connected to the protrusion at the center of the reversing valve 3-13 via a coupling located in the central opening of the mounting base. The sampling switching motor 2-1, the lead screw motor 3-9, and the reversing valve switching motor 3-12 are all oil-filled motors.

[0044] More detailed specific implementation examples:

[0045] In this specific example, the sample cabin is composed of a cabin shell 1-1, a cabin end cover 1-2, a sealing ring (or sealing strip), and three sub-sample cabins 1-6. The sample cabin is a cube, and the open end of the cabin shell 1-1 is provided with a groove for placing the end face sealing strip to form a seal with the cabin end cover 1-2. The cabin end cover 1-2 is connected to the cabin shell 1-1 by screws. The inner wall of the sub-sample cabin 1-6 is processed with a rectangular side wall hole 1-8, and the side wall hole 1-8 can cooperate with the side wall hole 2-4 of the sampling tube 2-3 to form a passage for seawater to enter the sub-sample cabin 1-6. The outer side of the side wall hole 1-8 is processed with a groove for placing the sealing ring to form a seal with the sampling tube 2-3. The sub-sample cabin 1-6 is composed of a sub-sample cabin body and a sub-sample cabin end cover 1-5. A sealing groove is provided at the open end of the cabin body for placing the sealing strip to form a seal with the end cover 1-5. The sub-sample cabin end cover 1-5 is connected to the cabin body by screws. The interior of the sample chamber is divided into four equal parts along two diagonal lines. The first sub-sample chamber 1-6-1, the second sub-sample chamber 1-6-2, and the third sub-sample chamber 1-6-3 each occupy a part, and the remaining part is left empty.

[0046] The sampling module 2 includes a sampling switching motor 2-1, a connecting tube 2-2 and a sampling tube 2-3. The sampling switching motor 2-1 adopts the form of an oil-filled motor, which is a relatively mature technology in the field of marine engineering. The motor cylinder is fixed to the connecting tube 2-2 by flange bolts, and the motor output shaft is connected to the boss at the tail end of the sampling tube 2-3 by a coupling. The sampling tube 2-3 is a circular tube with one end open, and a trumpet-shaped opening with a 120° angle is welded to the open end to increase the success rate of sampling. The closed end of the sampling tube 2-3 is machined with a boss to facilitate connection with the output shaft of the switching motor. The side wall of the sampling tube 2-3 is provided with a side wall hole 2-4. The sampling switching motor 2-1 drives the sampling tube 2-3 to rotate, so that the side wall hole 2-4 can be connected to the side wall hole 1-8 on any sub-sample tank 1-6, forming a passage for seawater and organisms to enter the sub-sample tank 1-6.

[0047] The preservation solution injection module 3 includes a screw motor 3-9, an injection piston 3-11, and a preservation solution storage cylinder 3-3. The screw motor 3-9 is an oil-filled motor, a relatively mature technology in the field of marine engineering. The screw 3-10 of the screw motor 3-9 is connected to the preservation solution injection piston 3-11 via a piston rod. When injecting the preservation solution, the screw motor 3-9 is activated to push out a certain volume of preservation solution, which is then injected into the target subsample chamber 1-6 through the multi-way valve 3-17. The switching function of the multi-way valve 3-17 is achieved by the reversing valve switching motor 3-12, the reversing valve 3-13, the multi-way valve inlet 3-7, and several multi-way valve outlet connectors 3-8. The bottom inlet 3-15 of the reversing valve 3-13 is connected to its side outlet 3-16. During installation, the reversing valve switching motor 3-12, reversing valve 3-13, and multi-way valve 3-17 are coaxially assembled. The reversing valve bottom inlet 3-15 is connected to the multi-way valve inlet connector 3-7 and sealed by a sealing ring. The reversing valve switching motor 3-12 drives the reversing valve 3-13 to rotate, so that the reversing valve lateral outlet 3-16 forms a coaxial passage with a multi-way valve outlet connector 3-8. A sealing ring is installed between the reversing valve lateral outlet 3-16 and the multi-way valve outlet connector 3-8.

[0048] The device described in the present invention also requires an oil-filled, encapsulated deep-sea battery and a control circuit board encapsulated in a pressure-resistant cabin. Since this is common knowledge and relatively mature technology in the field of marine engineering, it will not be described in detail here. In the present invention, the sample chamber, sampling tube, each motor chamber, connectors, sub-sample chamber, end caps, etc. can optionally be made of nylon to reduce weight. The injection mechanism connecting rod is made of stainless steel; the motor, joints, watertight connectors, couplings, etc. can all be commercially available products. The motor control scheme can determine the parameters of the control program according to actual needs. This is also common knowledge and relatively mature technology in this field and will not be described in detail here.

[0049] Usage examples:

[0050] Before launching the device, Figure 1Complete the installation of each module or component using the assembly method in , lift the injection piston 3-11 in the preservation liquid injection module 3 to the top of its stroke, and fill the preservation liquid storage cylinder 3-3 with preservation liquid. Use a PU hose to connect the preservation liquid storage cylinder outlet 3-6 to the multi-way valve inlet connector 3-7, and use a PU hose to connect the three multi-way valve outlet connectors 3-7 to the sample chamber inlet connectors 1-3 of the three sub-sample chambers 1-6 respectively. Place suitable baits for catching benthic organisms in the three sub-sample chambers 1-6. In order to prevent the sub-sample chamber 1-6 of the device from being damaged by external pressure after being launched into the water, deionized water needs to be injected into the sub-sample chamber 1-6 before launching. Then use a PU tube to connect all the oil-filling motors to the bladder compensator. Start the sampling switching motor 2-1 in the sampling module 2 to drive the sampling tube 2-3 to rotate, and rotate the sampling tube side wall hole 2-4 to the sample chamber to leave an empty space, such as Figure 3 As shown in .

[0051] Upon arrival at the designated operating area, the sampling switching motor 2-1 in the sampling module 2 is activated to rotate the sampling tube 2-3, aligning the side hole 2-4 of the sampling tube with the side hole 1-8 of the first sub-sampling chamber 1-6-1. This allows organisms in the seawater to enter the first sub-sampling chamber 1-6-1 through the bell-shaped opening of the sampling tube 2-3. Depending on the specific conditions of the seabed, a trapping time of 1-2 hours is typically sufficient to attract benthic organisms into the sub-sampling chamber before the bait is consumed.

[0052] After the scheduled sampling time is complete, the sampling switching motor 2-1 in the sampling module 2 is restarted to rotate the sampling tube 2-3 in the opposite direction to the reserved position, thereby sealing the first sub-sample chamber 1-6-1. The reversing valve switching motor 3-12 is activated to rotate the reversing valve 3-13, connecting the reversing valve lateral outlet 3-16 to the multi-way valve outlet connector 3-8 (which is connected to the sample chamber inlet connector 1-3 on the first sub-sample chamber 1-6-1). The screw motor 3-9 is activated to drive the injection piston 3-11 one-third of its stroke, injecting the preservation solution into the first sub-sample chamber 1-6-1 through the multi-way valve 3-17. After the injection is complete, the reversing valve switching motor 3-12 is restarted to rotate the reversing valve 3-13, closing the passage between the preservation solution storage cylinder 3-3 and the first sub-sample chamber 1-6-1. At this point, sampling and the injection of preservation solution into the first sub-sample chamber 1-6-1 are complete, allowing the biological sample to be preserved under appropriate conditions.

[0053] When the next sampling cycle begins, the sampling switching motor 2-1 in the sampling module 2 is activated to rotate the sampling tube 2-3, rotating the sampling tube sidewall hole 2-4 through the third sub-sample chamber 1-6-3 until it is connected to the corresponding sidewall hole 1-8 of the second sub-sample chamber 1-6-2. At this time, organisms in the seawater can enter the second sub-sample chamber 1-6-2 through the bell mouth of the sampling tube 2-3. After the scheduled sampling time is completed, the sampling switching motor 2-1 in the sampling module 2 is activated again to drive the sampling tube 2-3 to rotate in the opposite direction to the reserved position, thereby sealing the first sub-sample chamber 1-6-1. The reversing valve switching motor 3-12 is activated to drive the reversing valve 3-13 to rotate, connecting the reversing valve side outlet 3-16 to the multi-way valve outlet connector 3-8 (the multi-way valve outlet connector 3-8 is connected to the sample chamber inlet connector 1-3 on the second sub-sample chamber 1-6-2). The screw motor 3-9 is activated to drive the injection piston 3-11 one-third of its travel, injecting the preservation solution into the second sub-sample chamber 1-6-2 through the multi-way valve 3-17. After the injection is complete, the reversing valve switching motor 3-12 is activated again to rotate the reversing valve 3-13, cutting off the passage between the preservation solution storage cylinder 3-3 and the second sub-sample chamber 1-6-2. At this point, sampling and the injection of preservation solution into the second sub-sample chamber 1-6-2 are complete.

[0054] When the last sampling cycle is about to begin, the sampling switching motor 2-1 in the sampling module 2 is started to drive the sampling tube 2-3 to rotate, and the sampling tube side wall hole 2-4 is directly rotated to the corresponding side wall hole 1-8 of the third sub-sample compartment 1-6-3. At this time, organisms in the seawater can enter the third sub-sample compartment 1-6-3 through the bell mouth of the sampling tube 2-3. After the scheduled sampling time is completed, the sampling switching motor 2-1 in the sampling module 2 is started again to drive the sampling tube 2-3 to rotate in the opposite direction to the reserved position, thereby achieving the sealing of the first sub-sample compartment 1-6-1. The reversing valve switching motor 3-12 is started to drive the reversing valve 3-13 to rotate, so that the reversing valve side outlet 3-16 is connected to the multi-way valve outlet connector 3-8 (the multi-way valve outlet connector 3-8 is connected to the sample compartment inlet connector 1-3 on the third sub-sample compartment 1-6-3). The screw motor 3-9 is activated to drive the injection piston 3-11 one-third of its travel, injecting the preservation solution into the third sub-sample chamber 1-6-3 through the multi-way valve 3-17. After the injection is complete, the reversing valve switching motor 3-12 is activated again to rotate the reversing valve 3-13, cutting off the passage between the preservation solution storage cylinder 3-3 and the third sub-sample chamber 1-6-3. At this point, sampling and the injection of preservation solution into the three sample chambers are complete.

[0055] Finally, it should be noted that the above examples are merely specific embodiments of the present invention. Obviously, the present invention is not limited to the above examples and is subject to numerous variations. All variations that can be directly derived or conceived by a person of ordinary skill in the art from the disclosure of the present invention should be considered within the scope of protection of the present invention.

Claims

1. A long-term sampling and storage device for benthic organisms in deep-sea mining areas, characterized in that: It includes a sample chamber module, a sampling module, a preservation liquid injection module and a circuit switching module; wherein the sample chamber module includes a sample chamber composed of a chamber shell and a chamber end cover, as well as a reserved space and several sub-sample chambers arranged inside the sample chamber; the sampling module includes a motor cylinder, a sampling switching motor and a sampling tube; the preservation liquid injection module includes a motor cylinder, a screw motor, an injection piston and a preservation liquid storage cylinder; the circuit switching module includes a motor cylinder, a reversing valve switching motor, a reversing valve and a multi-way valve; wherein, In the sample chamber, the internal space of the sample chamber is divided evenly along the circumference and used as a reserved space and a placeholder for each sub-sample chamber. The sampling tube is longitudinally arranged in the center of the sample chamber, and each sub-sample chamber is fitted with the surface of the sampling tube through the inner wall arc surface; the bottom of the sampling tube is an open end, and its top closed end is connected to the output shaft of the sampling switching motor through a coupling; the sampling switching motor is fixed on the upper surface of the sample chamber and is used to drive the sampling tube to rotate around the axial direction; the side wall of the sampling tube is provided with a side wall hole, and the inner wall arc surface of each sub-sample chamber is provided with a side wall hole. Corresponding sidewall holes are provided on the upper surface for mutual communication; each group of sub-sample chambers is composed of a shell and an end cover, and the end cover is provided with a sample chamber inlet joint and a sample chamber outlet joint; the sidewall holes of the sampling tube and the sidewall holes of each sub-sample chamber have the same opening size and arrangement height; a sealing ring is installed in the sidewall hole of the sampling tube or the sidewall hole of the sub-sample chamber, and the side wall of the sampling tube fits with the inner wall arc surface of each sub-sample chamber to maintain a gap, so that the sampling tube can rotate around the axis while achieving sealing of each sub-sample chamber; A lead screw motor and a lead screw are provided inside the motor cylinder of the preservative liquid injection module, and an injection piston is provided inside the preservative liquid storage cylinder. The motor cylinder and the preservative liquid storage cylinder are connected end to end, and the lead screw motor is nested on the lead screw. The piston rod of the injection piston is connected to the lead screw, and the lead screw motor can drive the injection piston to move within the preservative liquid storage cylinder. A preservative liquid outlet connector and a preservative liquid inlet connector are provided at the end of the preservative liquid storage cylinder on the side opposite to the piston rod. A multi-way valve is installed at the open end of the motor cylinder of the loop switching module; a multi-way valve inlet connector is provided at the center of the end face of the multi-way valve, and several multi-way valve outlet connectors are provided on its side; a reversing valve is coaxially nested inside the multi-way valve, and an axial bottom inlet and a lateral outlet that are interconnected are provided inside the reversing valve, and the bottom inlet is connected to the multi-way valve inlet connector; a reversing valve switching motor is provided inside the motor cylinder, and its output shaft is fixedly connected to the axial center of the reversing valve through a coupling; the reversing valve switching motor can drive the reversing valve to rotate so that its lateral outlets are respectively connected to each multi-way valve outlet connector, thereby realizing multi-way output switching of the multi-way valve; the multi-way valve inlet connector is connected to the preservation liquid outlet connector through a pipeline, and each multi-way valve outlet connector is connected to the sample chamber inlet connector of each sub-sample chamber through a pipeline.

2. The device according to claim 1, characterized in that The shell of the sub-sample cabin is a hollow structure with one end open, and a sealing ring and an end cover are provided at the open end, and fixedly installed by screws; the cabin body shell of the sample cabin is a hollow structure with one end open, and a sealing ring and a cabin body end cover are provided at the open end, and fixedly installed by screws; a plurality of through holes are provided on the cabin body end cover for exposing the sample cabin inlet joint and the sample cabin outlet joint on the end cover of each sub-sample cabin.

3. The device according to claim 1, characterized in that The open end of the sampling tube is provided with a trumpet-shaped sampler.

4. The device according to claim 1, characterized in that In the sampling module, the sampling switching motor is fixedly installed in the motor cylinder, and the motor cylinder is fixedly installed on the upper surface of the sample chamber through the connecting cylinder.

5. The device according to claim 1, characterized in that The sample chamber, the reserved space and the sub-sample chamber have any one of the following structural features: (1) The transverse cross-section of the sample chamber is circular; the transverse cross-sections of the reserved spaces and each sub-sample chamber are fan-shaped; (2) The transverse cross-section of the sample chamber is a regular polygon; the inner edges of the transverse cross-sections of the reserved spaces and each sub-sample chamber are the same arc shape, and their outer edges are adapted to the shape of the sample chamber.

6. The device according to claim 1, characterized in that The motor cylinder and the preservation liquid storage cylinder in the loop switching module are installed in parallel in a fixed frame, and the fixed frame is installed on the cabin shell of the sample cabin.

7. The device according to claim 1, characterized in that The side wall holes of the sampling tube and the side wall holes of each sub-sample chamber are long rectangular or waist-shaped openings along the axial direction of the sampling tube.

8. The device according to claim 1, characterized in that When the preservative liquid storage cylinder is in a state of maximum volume, the end of the lead screw at the opposite end of the injection piston is close to the end of the motor cylinder.

9. The device according to claim 1, characterized in that The multi-way valve is fixed to the open end of the motor cylinder through a mounting base with a central opening. One end of the reversing valve switching motor is fixed to the mounting base and the other end is fixed to the motor cylinder. Its output shaft is fixedly connected to the protrusion in the center of the reversing valve through a coupling located in the central opening of the mounting base.

10. The device according to claim 1, characterized in that The sampling switching motor, the screw motor and the reversing valve switching motor are all oil-filled motors.

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