A cosmetic composition containing chia seed extract for strengthening the skin barrier and moisturizing.

Abstract

The cosmetic composition of the present invention includes low molecular weight peptides, including proline, hydroxyproline and lysine isolated from chia seeds. It has excellent effects on promoting the proliferation of skin fibroblasts and collagen synthesis, excellent skin moisturizing effect, and excellent skin barrier strengthening effect.

Description

Technical Field

[0001] This invention relates to a cosmetic composition containing chia seed extract for strengthening the skin barrier and moisturizing. Background Technology

[0002] The skin is a vital organ that plays a crucial role in protecting the body from external physical and chemical stimuli. The skin structure consists of three layers: the epidermis, dermis, and subcutaneous tissue. The epidermis itself is further divided into four layers: the stratum corneum, stratum granulosum, stratum spinosum, and stratum basale. The cells of the stratum corneum function similarly to the bricks themselves, while the lipids between the cells act like the mortar between the bricks, forming the skin barrier. The stratum corneum inhibits the evaporation of water from the body and prevents harmful substances from invading from the outside.

[0003] More than 70% of the skin's moisture resides in the dermis, decreasing to approximately 10%–30% in the epidermis. However, due to drastic environmental changes, irregular lifestyles, and aging, if the epidermis's moisture content falls below 10%, the skin surface becomes rough, loses its luster, and appears dull, making skin hydration increasingly important. Healthy individuals possess high concentrations of Natural Moisturizing Factor (NMF) in their keratinocytes, which helps the skin retain moisture. However, despite this function, external environmental stimuli such as indoor heating, polluted air, and ultraviolet radiation, as well as physical and chemical stimuli like friction, shaving, and face washing, can hinder and disrupt skin cell growth, leading to a breakdown of the skin barrier. To prevent this, strengthening the skin barrier and moisturizing become crucial, and various studies are underway to confirm their efficacy at the cellular level.

[0004] Skin is a complex bodily organ that simultaneously possesses the properties of an elastic solid and a viscous fluid; this mechanical property of skin is known as viscoelasticity. Viscoelasticity of the skin primarily occurs in the dermis, due to collagen, which occupies most of the matrix outside the dermal cells and plays a crucial role in maintaining skin strength and viscoelasticity. Collagen is the main structural protein accounting for 75% of the dry weight of skin. After being produced in the chief cells (fibroblasts) of the dermis, it is secreted into the extracellular matrix, maintaining skin elasticity through its triple helix structure. Therefore, if collagen is insufficient, the skin will experience decreased elasticity and hydration, leading to wrinkles and other signs of skin aging.

[0005] There are more than 20 types of collagen in the body. In the dermis of the skin, collagen type I accounts for 80-85%, and collagen type III accounts for 10-15%. The major amino acids proline and lysine, which are abundant in collagen, form a triple helix structure, which plays a role in maintaining skin elasticity. The regularity of the Gly-XY amino acid arrangement stabilizes its structure. Therefore, the synthesis of collagen types I and III, which affect skin structure, is believed to involve the participation of the major collagen-forming amino acids proline or hydroxyproline and lysine.

[0006] Maintaining proper skin cell growth and hydration is crucial. Therefore, cosmetics often incorporate ingredients similar to sebum, natural moisturizers (NMFs), or humectants like polyether polyols. For example, water-soluble polyether polyols containing three or more hydroxyl groups, such as glycerin and alcohols, possess excellent moisturizing power but are very sticky and can cause discomfort during use. Conversely, polyether polyols with two hydroxyl groups, such as propylene glycol and 1,3-butanediol, can also cause skin side effects. Furthermore, other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, and other elements, have strong electrolytes that can impair the emulsification stability of cosmetics. While collagen and elastin also have moisturizing capabilities, their absorption capacity is limited. Therefore, to strengthen the skin barrier, it is necessary to develop natural cosmetic compositions.

[0007] Prior literature regarding the moisturizing effects of compositions containing peptides has been published.

[0008] Korean Patent No. 249202 discloses a pharmaceutical composition for the prevention or treatment of xeroderma, including ZAG protein-derived peptides.

[0009] Korean Patent No. 10-1074776 discloses a method for separating the proteoglycan layer present on the surface of chia seeds and initiating the manufacture of a polymer, as well as a composition containing this component. The aforementioned composition has moisturizing effects, among other benefits. Summary of the Invention

[0010] The purpose of this invention is to overcome the shortcomings of existing products and provide a cosmetic composition for strengthening the skin barrier and moisturizing the skin, containing chia seed peptide as an effective ingredient.

[0011] To solve the above-mentioned technical problems, the present invention provides the following technical solution:

[0012] This invention discloses a cosmetic composition for strengthening the skin barrier and moisturizing, which contains peptides isolated from chia seeds.

[0013] Preferably, the peptides isolated from the chia seeds include low molecular weight peptides with a molecular weight of less than 500 Da.

[0014] Preferably, the peptides isolated from the chia seeds include one or more of proline, lysine, and hydroxyproline.

[0015] Preferably, the peptides isolated from the chia seeds have the effect of promoting the proliferation of dermal fibroblasts or the synthesis of collagen.

[0016] Preferably, the peptides isolated from the chia seeds contain 0.1% to 30.0% of the total weight of the cosmetic composition.

[0017] Preferably, the cosmetic composition is any basic cosmetic material formulation selected from the group consisting of solutions, suspensions, emulsions, creams, lotions, gels, water-soluble liquids, face creams, serums, cleansers containing surfactants, oils, oil-in-water emulsions, and water-in-oil emulsions; mask formulations; shower gel formulations; gels; oil-in-water or water-in-oil emulsion foundations; concealers; lipsticks; lip glosses; pressed powders; dual-effect pressed powders; eyeshadows; blushes; and eyebrow pencils; or a scalp formulation.

[0018] The beneficial effects of the present invention are: the cosmetic composition of the present invention includes low molecular weight peptides including proline, hydroxyproline and lysine isolated from chia seeds, which have excellent effects on promoting the proliferation of skin fibroblasts and collagen synthesis, excellent skin moisturizing effect and excellent skin barrier strengthening effect. Attached Figure Description

[0019] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used in conjunction with embodiments of the invention to explain the invention and do not constitute a limitation thereof. In the drawings:

[0020] Figure 1 This shows one of the results of the amino acid analysis of chia seed peptides.

[0021] Figure 2 The second result of the amino acid analysis of chia seed peptides is shown.

[0022] Figure 3 The results of hydroxyproline analysis of chia seed peptides are shown.

[0023] Figure 4 The results of the analysis of chia seed peptide molecular weight are shown.

[0024] Figure 5 The results show the effect of chia seed peptide on the proliferation of dermal fibroblasts.

[0025] Figure 6 The results indicate the collagen synthesis-promoting effect of chia seed peptides. Detailed Implementation

[0026] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are for illustration and explanation only and are not intended to limit the present invention.

[0027] This invention relates to a cosmetic composition for improving the skin barrier and moisturizing the skin, comprising peptides isolated from chia seeds as active ingredients.

[0028] Chia seeds (Salvia hisperia) are a flowering plant belonging to the mint family, native to south-central Mexico and Guatemala. Cultivated for seed harvesting, they have been used for centuries in South America, western Mexico, and the southwestern United States, and are frequently used as food and functional foods. Chia seeds are hydrophilic, capable of absorbing approximately 12 times their weight in water. Nutritionally, 100g of chia seeds contains about 42g of carbohydrates, 30g of fat, 16g of protein, and other minerals. Omega-3 fatty acids, such as linolenic acid, account for about 25-30%. Furthermore, unlike other grains such as wheat, corn, rice, and oats, chia seeds contain a high amount of protein and have strong anti-inflammatory effects.

[0029] The presence of active ingredients in this invention means that the cosmetic composition of this invention can exhibit effects of strengthening the skin barrier and moisturizing the skin. This means that chia seed peptides are added to the cosmetic composition. In order to deliver the ingredients and stabilize them, multiple ingredients are added to the secondary ingredients, that is, including formulation in multiple forms.

[0030] In the cosmetic composition of the present invention, the aforementioned chia seed peptide can be manufactured according to conventional methods published in the industry, i.e., under normal solvent, temperature, and pH conditions, using common proteolytic enzymes. Preferably, it can be manufactured using a single or mixed solvent of alcohol and purified water, at a temperature of 20-60°C and a pH below 5, employing food-grade acidic or neutral proteolytic enzymes. Most preferably, it can be manufactured using a 100% purified water solvent, at a temperature of 40-45°C, with the pH adjusted to 2-4, utilizing an enzymatic decomposition process involving a mixture of papain, pepsin, and an enzyme in a 1:1 weight ratio.

[0031] Furthermore, the chia seed peptides of the present invention include not only the extraction methods described above, but also substances that have typically undergone purification. Preferably, substances obtained by various purification methods such as alcohol solvent partitioning, centrifugation, precision filtration membrane, ultrafiltration membrane separation, and various chromatographic separations (1-4) are also included in the present invention.

[0032] Furthermore, the chia seed peptides of the present invention include not only the extraction and purification methods described above, but also substances that have typically undergone a drying process. Preferably, substances obtained by various drying methods such as concentration, hot air drying, spray drying, and freeze drying are also included in the present invention.

[0033] Chia seed peptides manufactured using the above-described manufacturing method of the present invention comprise low molecular weight peptides. Preferably, substances with a molecular weight range of less than 500 Da are included in the present invention.

[0034] In this invention, chia seed peptide is characterized by including proline, hydroxyproline and lysine.

[0035] In addition, in the above composition, chia seed peptides have the effect of promoting the proliferation of dermal fibroblasts and collagen synthesis, thus exhibiting a skin moisturizing effect.

[0036] The chia seed peptides mentioned above can be present in the total weight of the cosmetic composition from 0.1% to 30.0%. Ideally, they should be present in the total weight of the cosmetic composition from 0.1% to 10.0%. When the content of the above extract is less than 0.1%, the effect is negligible; when it exceeds 30.0%, the increase in effect due to the increased content is negligible, and there are issues with the safety and stability of the dosage form, making it uneconomical.

[0037] On the other hand, the compositions of the present invention can be manufactured from any dosage form commonly manufactured in the industry. For example, solutions, suspensions, emulsions, creams, lotions, gels, water-soluble liquids, face creams, serums, basic cosmetic dosage forms containing surfactants—cleansers, oils, oil-in-water (O / W) and water-in-oil (W / O) types; toners; lotions; face creams; eye creams; soothing gels; ointments; mask dosage forms; shower gel dosage forms; gels; oil-in-water or water-in-oil foundations; foundation creams; concealers; lipsticks; lip glosses; pressed powders; dual-effect pressed powders; eyeshadows; blushes and eyebrow pencils; or dosage forms for the scalp.

[0038] Furthermore, the cosmetic compositions of the present invention utilize auxiliary agents commonly used in the cosmetic field, such as hydrophilic or lipophilic surfactants, preservatives, antioxidants, solvents, fragrances, fillers, separating agents, pigments, absorbents, dyes, modifiers, etc. The amounts of these different auxiliary agents are generally those commonly used in the field; however, in all cases, the auxiliary agents and their proportions will be selected so as not to affect the desirable properties of the cosmetic compositions brought about by the present invention.

[0039] Furthermore, the cosmetic composition of the present invention can be reused with other cosmetic compositions besides those of the present invention. Additionally, the cosmetic composition according to the present invention can be used according to the usual method of application, and the number of times it can be used may vary depending on the user's skin condition or preference.

[0040] Furthermore, according to the present invention, the cosmetic composition containing chia seed peptides exhibits excellent effects in promoting dermal fibroblast proliferation and collagen synthesis, and also provides skin moisturizing effects. In addition, because it uses natural materials, it is harmless to the human body and is a cosmetic composition with excellent safety.

[0041] The present invention will now be described in detail with reference to preparation examples and embodiments, but the scope of the invention is not limited by the preparation examples and embodiments.

[0042] [Preparation Example 1] Manufacturing of Chia Seed Peptides

[0043] The chia seed peptide powder mentioned above is manufactured by the following method.

[0044] (1) Chia seeds are powdered: Chia seeds are powdered using a crusher and pulverizer.

[0045] (2) Enzymatic hydrolysis of proteins: Soak the above chia seed powder in purified water at a concentration 20 times its weight, maintain a temperature of 40-45°C, adjust the pH to 2-4, and add 0.5%-2% of the weight of the chia seed powder to a 1:1 mixture of papain and pepsin. Stir for 6 hours to allow the enzyme to decompose, boil for 30 minutes to remove activity, add 1M citric acid, and adjust the pH to 5-7. After standing at room temperature for 24 hours, remove the precipitate through a filter screen of less than 400 mesh, and centrifuge to obtain the supernatant.

[0046] (3) Separation and purification: The upper liquid is reduced by 1 / 10 of its volume by depressurization and concentration. Ethanol is added at a 1:1 ratio to the concentrate, and the mixture is placed at low temperature for 24 hours. The upper liquid is then obtained by centrifugation.

[0047] (4) Filtration: The separated upper liquid is passed through a precision filter membrane of 0.1~0.5 micrometers to obtain filtrate.

[0048] (5) Drying: Freeze-dry the filtrate at -40℃ to 40℃ to obtain chia seed peptide powder.

[0049] [Comparative Preparation Example 1] Preparation of Chia Seed Solvent Extract

[0050] The above-mentioned chia seed solvent extract is manufactured by the following method.

[0051] (1) Chia seeds are powdered: Chia seeds are powdered using a crusher and pulverizer.

[0052] (2) Solvent extraction: Immerse the above chia seed powder in 20 times its weight of pure water and stir at 300 rpm at room temperature.

[0053] (3) Filtration: The extract was filtered through a 400-mesh screen, and the filtrate was centrifuged at 7000 rpm for 15 minutes to obtain the supernatant.

[0054] (4) Drying: Freeze-dry the upper liquid at -40℃~40℃ to obtain chia seed extract powder.

[0055] [Experimental Example 1] Amino Acid Analysis

[0056] To confirm the presence of proline and lysine, which play important roles in skin collagen synthesis, amino acid analysis was performed using HPLC.

[0057] (1) Sample pretreatment: Take an appropriate amount of chia seed peptide from Preparation Example 1, add 6N HCl, hydrolyze at 120 degrees Celsius for 24 hours, dilute to pure water, and filter with 0.45μm filter paper.

[0058] (2) Analysis conditions

[0059] - Column: Cation column

[0060] - Detector: UV / Vis detector 340 nm, 450 nm / Fluorescence 266 nm, 305nm

[0061] - Mobile Phase: 40mM Sodiumphosphate(Di basic) 0.1% Phosphoric acid(A)

[0062] Acetonitrile / Methanol / DW=45 / 45 / 10(B)

[0063] [Table 1]

[0064] Time A B 0.0 100 0 1.9 100 0 18.1 43 57 18.6 20 80 22.3 20 80 23.2 100 0 26.0 100 0

[0065] - Flow: 1.5 ml / min

[0066] - Temperature: 40℃

[0067] exist Figure 1 and 2 The results of amino acid analysis of chia seed peptides are shown. It was confirmed that chia seed peptides contain 5.97 mg / L of proline and 22.48 mg / L of lysine. The presence of proline and lysine, which play important roles in collagen formation, helps strengthen the skin barrier.

[0068] [Experimental Example 2] Hydroxyproline Analysis

[0069] To confirm the presence of hydroxyproline, which plays an important role in skin collagen synthesis, amino acid analysis was performed using HPLC.

[0070] (1) Sample pretreatment: Take an appropriate amount of chia seed peptide from Preparation Example 1, dilute it with purified water, and filter it with 0.45μm filter paper.

[0071] (2) Analysis conditions

[0072] -Column: A stainless steel tube with an inner diameter of approximately 4.6 mm and a length of approximately 25 cm is filled with 5 mm of high-performance liquid chromatography silica gel.

[0073] - Detector: UV / Vis detector 203 nm

[0074] - Mobile Phase: Water

[0075] [Table 2]

[0076] No. Time Flow % purified water 1 0 0.4 100 2 10 0.4 100

[0077] - Flow: 0.4 ml / min - Temperature: 30℃ Figure 3 The results of the chia seed peptide analysis for hydroxyproline are shown.

[0078] It has been confirmed that chia seed peptides contain an average of 0.35% hydroxyproline. Containing hydroxyproline, which plays an important role in collagen formation, helps strengthen the skin barrier.

[0079] [Experimental Example 3] Molecular Weight Analysis

[0080] To confirm the molecular weight of chia seed peptides, MALDI-TOF MS was used for analysis.

[0081] The dried sample of chia seed peptide from Preparation Example 1 was dissolved in a 50% [v / v] methanol / water mixture, and 1 μL of the solution was mixed with 1 μL of SA (sinapinic acid) matrix (50% [v / v] 0.1% TFA acetonitrile / 50% water). The mixture was spotted onto a stainless steel MALDI plate and dried at room temperature. Analysis was performed using Microflex LRF MALDI-TOF mass spectrometry and Bruker UltrafleXtreme (Bruker Daltonics, Bremen, Germany) in reflector mode.

[0082] The intensities of each ion were calculated by summing the peaks from the first isotope region to the third isotope region. Spectral acquisition and processing were performed using FlexAnalysis software (ver. 3.3, Booker Daltonics, Bremen, Germany), and the results are shown in [the table / image / data]. Figure 4 middle.

[0083] like Figure 4 As shown, the molecular weight of chia seed peptides is below 500 da, confirming the presence of low molecular weight peptides.

[0084] [Experimental Example 4] Evaluation of Skin Fibroblast Proliferation

[0085] Human deral fibroblasts (HDFn) cells were busy at 1 x 10⁴ cells / well in 96-well plates and cultured for one day in an incubator at 37°C with 5% CO₂. Then, the incubator was switched to one containing 0.2% FBS. Serially diluted samples were treated according to concentration and reacted in medium at 37°C for 48 hours. 20 μL of MTT reagent was added to each well, and after reacting in medium for 2 hours, the supernatant was removed, and 100 μL of DMSO was added to completely dissolve the cells. The absorbance was measured at 550 nm. The significance of the test substance was then confirmed using a two-sample t-test according to biostatistical criteria (significance level (α): 1%). The results were displayed as... Figure 5 middle.

[0086] like Figure 5 As shown, when treating Preparation Example 1 and Comparative Preparation Example 1 respectively, Preparation Example 1 showed a significant effect, with an increase of approximately 33% at the maximum concentration of 100 ug / ml, confirming the skin fibroblast proliferation effect of chia seed peptide.

[0087] [Experimental Example 5] Effect on promoting collagen production

[0088] 1) Cell Culture and Sample Preparation: Dermatologic fibroblasts were added to F12 / DMEM medium containing 10% FBS. 1×10⁵ cells / well were added to 450 ml of medium in a 24-well plate and cultured for 24 hours. Different concentrations of sample were added to each well, and the mixture was incubated for another 24 hours at 37°C with 5% CO₂. The supernatant was then collected, and collagen content was measured using the Procollagen Type I peptide EIA kit (Takara, Japan). Protein content was confirmed using the BCA quantification method and corrected using collagen content per unit cell. After washing cells with PBS, the cells were treated with 1X RIPA buffer containing protease inhibitors to lyse them. The supernatant was then recovered by centrifugation.

[0089] 2) Protein quantification: Add BCA solution (A:B=50:1) to the recovered supernatant. After reacting for 30 minutes, measure the absorbance from 540 nm to the ELISA reader. Substitute the measured value into the standard detection line of albumin (0~2 mg / ml) to obtain the protein content. Calculate the total protein content according to the following formula.

[0090] 3) Collagen content determination: The Antibody-POD conjugate solution was placed in a well, and the supernatant (separated from the standard solution) was added. The mixture was incubated for 3 hours. After removing the reaction solution from the well, the sample was washed four times with PBS. A chromogenic reagent was added, and the mixture was reacted at room temperature for 15 minutes. Then, 1N sulfuric acid was added, and the absorbance was measured at 492 nm. The calculated collagen synthesis rate, compared to the control group, was also obtained. Figure 6 middle.

[0091] like Figure 6 As shown, when treating Preparation Example 1 and Comparative Preparation Example 1 respectively, Preparation Example 1 showed a significant effect, with an increase of approximately 65% ​​at the maximum concentration of 100 ug / ml, confirming the collagen synthesis-promoting effect of chia seed peptides.

[0092] [Example 1] Manufacturing products using chia seed peptides

[0093] Cosmetic compositions containing chia seed peptides, as shown in Table 3, are manufactured according to conventional methods.

[0094] [Table 3]

[0095]

[0096] [Experimental Example 6] Measurement of transdermal water loss

[0097] Eighteen participants aged 20-40 years without skin diseases were enrolled, divided into three groups of six. Each group applied the cosmetic materials described in Example 1 and Comparative Examples 1 and 2 to their face and skin. Under constant temperature and humidity conditions (24°C, 40% humidity) before application, the amount of skin moisture evaporation was measured using a Tewameter TM210 (Courage and Kazaka, Germany). Measurements were taken at 1 hour, 2 hours, 4 hours, and 6 hours after application, and the reduction rate (%) was evaluated. The results are shown in Table 4 below.

[0098] [Table 4]

[0099]

[0100] As shown in Table 4, Example 1, which contains chia seed peptides, had a lower rate of transdermal moisture reduction compared to Comparative Examples 1 and 2.

[0101] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A cosmetic composition for strengthening the skin barrier and moisturizing, characterized in that: It contains peptides isolated from chia seeds; The peptides isolated from the chia seeds include low molecular weight peptides with a molecular weight of less than 500 Da. The peptides isolated from chia seeds include one or more of proline, lysine, and hydroxyproline. The peptides isolated from chia seeds are manufactured using the following method: (1) Powdering of chia seeds: Chia seeds are powdered using a crusher and pulverizer; (2) Enzymatic hydrolysis of protein: Soak the above chia seed powder in pure water, maintain the temperature at 40~45℃, adjust the pH to 2~4, add 0.5%~2% of the weight of the chia seed powder to the enzyme mixture of papain and pepsin in a 1:1 ratio; stir to allow the enzyme to decompose, boil for 30 minutes, remove the activity, add 1M citric acid, and adjust the pH to 5~7; after standing at room temperature for 24 hours, remove the precipitate through a filter screen of less than 400 mesh, and centrifuge to obtain the supernatant; (3) Separation and purification: The upper liquid is reduced and concentrated, and ethanol is added at a ratio of 1:1 to the concentrate. After standing at low temperature for 24 hours, the upper liquid is obtained by centrifugation. (4) Filtration: The separated upper layer liquid is passed through a precision filter membrane of 0.1~0.5 micrometers to obtain filtrate; (5) Drying: Freeze-dry the filtrate at -40℃ to 40℃ to obtain chia seed peptide powder.

2. The cosmetic composition for strengthening the skin barrier and moisturizing according to claim 1, characterized in that: The peptides isolated from chia seeds have the effect of promoting the proliferation of dermal fibroblasts or the synthesis of collagen.

3. The cosmetic composition for strengthening the skin barrier and moisturizing according to claim 1, characterized in that: The peptides isolated from the chia seeds account for 0.1% to 30.0% of the total weight of the cosmetic composition.

4. The cosmetic composition for strengthening the skin barrier and moisturizing according to any one of claims 1 to 3, characterized in that: The cosmetic composition is one of the group consisting of shower gel, water-in-oil or oil-in-water foundation, concealer, lipstick, lip gloss, eyeshadow, blush, or eyebrow pencil.

5. The cosmetic composition for strengthening the skin barrier and moisturizing according to any one of claims 1 to 3, characterized in that: The cosmetic composition is a facial powder.

6. The cosmetic composition for strengthening the skin barrier and moisturizing according to any one of claims 1 to 3, characterized in that: The cosmetic composition is one of cream, gel, face cream, serum or face mask.

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