A traditional Chinese medicine for preventing and treating retinal fibrous membrane or neovascularization proliferative disease

Abstract

The application discloses a traditional Chinese medicine for preventing and treating retinal fibrous membrane or neovascularization proliferative disease, which is composed of leech, zedoary, panax notoginseng, earthworm, kelp and turtle shell. Compared with the prior art, the medicine has wide sources, small toxic and side effects and high safety. The application provides a new method, a new idea and a new prospect for clinical application for the traditional Chinese medicine for preventing and treating retinal fibrous membrane or neovascularization proliferative disease.

Description

Technical Field

[0001] This invention relates to the field of ophthalmic drug technology, and in particular to a traditional Chinese medicine for the prevention and treatment of retinal fibrosis or neovascularization. Background Technology

[0002] Retinal fibrosis or neovascularization is a common pathological change in many fundus diseases and a fundamental reason for surgical failure or poor treatment results in fundus diseases. Currently, the treatment of such diseases often involves intraocular injection of anti-VEGF drugs, which often requires multiple injections and repeated treatments. The treatment is expensive, costly, and risky, and the long-term efficacy is uncertain. There is also a lack of treatment methods that can effectively control the formation of proliferative membranes or neovascularization. Summary of the Invention

[0003] The purpose of this invention is to provide a traditional Chinese medicine for the prevention and treatment of retinal fibrosis or neovascularization.

[0004] To achieve the above objectives, the present invention is implemented according to the following technical solution:

[0005] This invention is composed of leeches, turmeric, Panax notoginseng, earthworm, kelp, and turtle shell.

[0006] Preferably, the ingredients are 3g of leech, 10g of turmeric, 6g of Panax notoginseng, 20g of earthworm, 20g of kelp, and 20g of turtle shell.

[0007] The preparation method of this invention includes the following steps:

[0008] S1: Soak leeches, turmeric, notoginseng, earthworm, and kelp in cold water;

[0009] S2: Boil the turtle shell and then boil the soaked medicine from step S1 together.

[0010] S3: After decocting, filter out the liquid and set aside. Add water again and decoct.

[0011] S4: The medicinal juice from step S2 and step S3 is mixed to obtain a traditional Chinese medicine for preventing and treating retinal fibrosis or neovascularization.

[0012] Preferably, in step S1, the soaking time is 30-60 minutes, and the amount of cold water used is such that the water level covers the herbs by about 3-5 cm. The decoction time of the turtle shell is 20-30 minutes, and the total decoction time is 20-30 minutes. In step S3, 400 ml of decoction is filtered out, and water is added again to decoct and filter out another 400 ml of decoction.

[0013] The beneficial effects of this invention are:

[0014] This invention relates to a traditional Chinese medicine approach for the prevention and treatment of retinal fibrosis or neovascularization. Compared with existing technologies, this invention utilizes widely available medicinal materials, has fewer toxic side effects, and is highly safe. It provides new methods and ideas for the prevention and treatment of retinal fibrosis using traditional Chinese medicine, and offers new prospects for clinical application. Detailed Implementation

[0015] The present invention will be further described below with reference to specific embodiments. The illustrative embodiments and descriptions herein are used to explain the present invention, but are not intended to limit the present invention.

[0016] The entire formula consists of 3g of leech, 10g of turmeric, 6g of Panax notoginseng, 20g of earthworm, 20g of kelp, and 20g of turtle shell.

[0017] The preparation method of this invention includes the following steps:

[0018] S1: Soak leeches, turmeric, notoginseng, earthworm, and kelp in cold water for 30-60 minutes; the amount of cold water should be enough to submerge the herbs by about 3-5 cm.

[0019] S2: Boil the turtle shell for 20-30 minutes, then boil the soaked medicine from step S1 together for another 20-30 minutes.

[0020] S3: After decocting, filter out 400ml of the decoction for later use. Add water again, decoct, and filter out another 400ml of the decoction.

[0021] S4: The medicinal juice from step S2 and step S3 is mixed to obtain a traditional Chinese medicine for preventing and treating retinal fibrosis or neovascularization.

[0022] The formula uses leeches and turmeric as the chief herbs, primarily to break up blood stasis and eliminate masses; Panax notoginseng and earthworm as assistant herbs, to invigorate blood and unblock the meridians to aid in the elimination of masses; kelp as adjuvant herbs, to soften hardness and dissipate nodules; and turtle shell as guiding herbs, to nourish yin and dissipate nodules, while also mitigating the excessive stagnation of blood-breaking herbs that could damage yin. The combined effects of these herbs are to break up blood stasis, eliminate masses, soften hardness, and dissipate nodules, thereby inhibiting the proliferation of fibrous tissue. Treatment principle: Break up blood stasis, eliminate masses, soften hardness, and dissipate nodules. Mainly treats: myopic macular degeneration, age-related macular degeneration, diabetic retinopathy, rhegmatogenous retinopathy, posterior segment ocular trauma, inflammatory diseases of the retinal vessels, idiopathic choroidal neovascularization, and proliferative vitreoretinopathy, etc.

[0023] Efficacy Results: In vitro and in vivo experiments were conducted to investigate whether the method of breaking up blood stasis and eliminating masses could effectively inhibit retinal fibrosis or neovascularization. The results showed that:

[0024] 1. In vitro culture of RPE cells showed that the blood-breaking and mass-eliminating method may effectively upregulate Caspase-3 protein expression and downregulate Cyclin D1, p38MAPK, ERK, and PI3K protein expression by regulating the p38MAPK, ERK, and PI3K signaling pathways, thereby prolonging the cell cycle, inhibiting cell proliferation, and inducing apoptosis.

[0025] 2. Building upon cell experiments, in vivo experiments were conducted to investigate the intervention effects of the traditional Chinese medicine compound "Po Xue Xiao Zheng" on traumatic retinal retinopathy (PVR) in rabbits, examining imaging, pathological cytology, and protein expression. Blue rabbits were selected as experimental animals. The model was established through traumatic incision and intravitreal injection of allogeneic plasma. Twenty-eight days after successful modeling, the "Po Xue Xiao Zheng" compound effectively reduced vitreous opacity, inhibited fibrous tissue production, and decreased local retinal contraction and scar formation, thus preventing complications such as retinal detachment. HE staining results showed that the "Po Xue Xiao Zheng" compound effectively reduced the infiltration of inflammatory cells in various layers of the retina and decreased the production of proliferative tissue, thereby preserving the integrity of the retinal morphology and structure. Western blot and PCR analysis of protein expression on the retinal proliferative membrane showed that the "blood-breaking and swelling-reducing" method may effectively regulate the p38MAPK, PI3K, and ERK pathways to upregulate Caspase-3 protein expression in the retinal proliferative membrane of traumatized PVR rabbits, downregulate Cyclin D1, p38MAPK, ERK, and PI3K protein expression, inhibit cell proliferation, and promote cell apoptosis, thereby maintaining the normal morphology and structure of the retina and preventing the occurrence and development of retinal fibrosis or neovascularization.

[0026] 3. The results of in vitro and in vivo experiments are consistent, both indicating that the method of breaking blood stasis and eliminating masses can effectively prevent and treat the occurrence and development of retinal fibrosis or neovascularization. It has theoretical support and clinical guiding significance for the prevention and treatment of fundus retinal fibrosis or neovascularization by traditional Chinese medicine, and is conducive to better leveraging the unique advantages of traditional Chinese medicine.

[0027] The technical solutions of the present invention are not limited to the specific embodiments described above. Any technical modifications made in accordance with the technical solutions of the present invention fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for preventing and treating proliferative retinal fibrosis, characterized in that: It is made from 3g of leech, 10g of turmeric, 6g of Panax notoginseng, 20g of earthworm, 20g of kelp, and 20g of turtle shell.

2. The traditional Chinese medicine composition for preventing and treating retinal fibrosis according to claim 1, characterized in that, The preparation method includes the following steps: S1: Soak leeches, turmeric, notoginseng, earthworm, and kelp in cold water; S2: Boil the turtle shell and then boil the soaked medicine from step S1 together. S3: After decocting, filter out the medicinal juice and set it aside. Add water again and decoct. S4: The medicinal juice from step S2 and step S3 is mixed to obtain a traditional Chinese medicine composition for preventing and treating proliferative diseases of the retinal fibrosis membrane.

3. The traditional Chinese medicine composition for preventing and treating retinal fibrosis according to claim 2, characterized in that: The soaking time in step S1 is 30-60 minutes, and the amount of cold water used is such that the water level is about 3-5 cm above the surface of the medicine.

4. The traditional Chinese medicine composition for preventing and treating retinal fibrosis according to claim 2, characterized in that: The turtle shell is simmered for 20-30 minutes, and the total simmering time is 20-30 minutes.

5. The traditional Chinese medicine composition for preventing and treating retinal fibrosis according to claim 2, characterized in that: In step S3, 400ml of medicinal juice is filtered out, and water is added again to decoct and filter out another 400ml of medicinal juice.