Reagent filling device and method

Abstract

The present invention discloses a reagent filling device and method, wherein the reagent filling device includes a liquid supply power device and a first liquid storage tank and a second liquid storage tank connected to the liquid supply power device, the first liquid storage tank and the second liquid storage tank are arranged in parallel and selectively connected to the liquid supply power device, the liquid supply power device first fills a certain dose of reagent into the first liquid storage tank to discharge the air in the liquid supply power device into the first liquid storage tank, and then fills the second liquid storage tank with reagent, the first liquid storage tank is used to provide reagent to the bubble-insensitive module, and the second liquid storage tank is used to provide reagent to the bubble-sensitive module. The present invention empties the front air during the process of filling the reagent into the first liquid storage tank, ensuring that the reagent subsequently filled into the second liquid storage tank does not contain air, thereby ensuring the accuracy of the sample detection results.

Description

Technical Field

[0001] The present invention relates to the technical field of sample analyzers, and in particular to a reagent filling device and method for a sample analyzer. Background Art

[0002] The most commonly used hematology analyzer, a sample analyzer, uses reagents to perform statistical analysis on various cells in a blood sample, providing a basis for doctors' diagnosis and treatment. Some high-end sample analyzers use a reservoir to cache the reagents needed for testing. During operation, the sample analyzer draws the reagents directly from the reservoir, speeding up testing.

[0003] Typically, sample analyzers are equipped with an independent reagent filling system to promptly fill and replenish reagents into the liquid reservoir to ensure smooth sample testing. Existing reagent filling systems primarily utilize negative pressure aspiration and quantitative pumping. Regardless of the method, air is inevitably pumped into the liquid reservoir at the beginning of the filling process, resulting in small bubbles in the reagents injected into the reservoir.

[0004] As the reagent flows within the sample analyzer, small bubbles adhere to the walls of the instrument's internal pipes or key detector components, affecting the stability of the sample flow path or optical path. In addition, if the size of the small bubbles is close to the size of the target particles to be measured, it will cause instrument recognition errors and affect the reliability of the test results. Therefore, it is necessary to improve the reagent filling system and method of existing sample analyzers. Summary of the Invention

[0005] In view of this, a reagent filling device and a reagent filling method are provided that can effectively avoid the generation of small bubbles.

[0006] The present invention provides a reagent filling device, comprising a liquid supply power device and a first liquid storage tank and a second liquid storage tank connected to the liquid supply power device. The first liquid storage tank and the second liquid storage tank are arranged in parallel and selectively connected to the liquid supply power device. The liquid supply power device first fills a certain dose of reagent into the first liquid storage tank to discharge the air in the liquid supply power device into the first liquid storage tank, and then fills the second liquid storage tank with reagent. The first liquid storage tank is used to provide reagent to the bubble-insensitive module, and the second liquid storage tank is used to provide reagent to the bubble-sensitive module.

[0007] Furthermore, the liquid supply power device includes a syringe, which is respectively connected to the first liquid storage tank and the second liquid storage tank; or, the liquid supply power device includes a pressure source and a liquid storage tank, the pressure source is connected to the liquid storage tank through a switching member, and the liquid storage tank is respectively connected to the first liquid storage tank and the second liquid storage tank.

[0008] Furthermore, the liquid supply power device includes a first pressure source and a metering pump, the first pressure source is connected to the metering pump through a first switching component, and the metering pump is connected to the first liquid storage tank and the second liquid storage tank respectively.

[0009] Furthermore, the metering pump is connected to the first liquid storage tank and the second liquid storage tank via a second switching element.

[0010] Furthermore, a reagent container is included, and the reagent container is connected to the metering pump through a one-way valve.

[0011] Further, the reagent container is connected between the second switching element and the first liquid storage tank; or, the reagent container is connected between the metering pump and the second switching element.

[0012] Furthermore, the reagent container is connected to the first liquid storage tank via the one-way valve, and a switching piece is connected between the reagent container and the first liquid storage tank.

[0013] Furthermore, the first liquid storage tank is also connected to a second pressure source.

[0014] Furthermore, a third switching element is connected between the second pressure source and the first liquid storage tank.

[0015] The present invention also provides a reagent filling method comprising the following steps: S1, connecting the liquid supply power device with the first liquid storage tank and disconnecting the liquid supply power device with the second liquid storage tank, and filling the first liquid storage tank with reagent until the air in the liquid supply power device is emptied; S2, connecting the liquid supply power device with the second liquid storage tank and disconnecting the liquid supply power device with the first liquid storage tank, and filling the second liquid storage tank with reagent.

[0016] Compared with the existing technology, the present invention sets up two liquid storage tanks and fills reagents into the two liquid storage tanks successively. In the process of filling the reagent into the first liquid storage tank, the front air is emptied, and the reagent is poured into the second liquid storage tank after the front air is exhausted, ensuring that the reagent in the second liquid storage tank does not contain bubbles. It can be used in sample testing to ensure the accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic structural diagram of the first embodiment of the reagent filling device of the present invention.

[0018] Figure 2 This is a schematic structural diagram of the second embodiment of the reagent filling device of the present invention.

[0019] Figure 3 This is a schematic structural diagram of the third embodiment of the reagent filling device of the present invention. DETAILED DESCRIPTION

[0020] To facilitate understanding of the present invention, the present invention will be described more fully below with reference to the accompanying drawings. The accompanying drawings illustrate one or more embodiments of the present invention to provide a more accurate and thorough understanding of the technical solutions disclosed herein. However, it should be understood that the present invention can be implemented in a variety of different forms and is not limited to the embodiments described below.

[0021] The same or similar numbers in the drawings of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are terms such as "upper", "lower", "left", "right", etc. indicating directions or positional relationships, they are based on the directions or positional relationships shown in the drawings. This is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and cannot be understood as limiting this patent. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0022] The present invention provides a reagent filling device and a reagent filling method based on the device, which are applied to a sample analyzer to fill reagents from reagent containers, such as reagent bottles, into a liquid storage tank for temporary storage, so that the sample analyzer can directly obtain reagents from the liquid storage tank when performing sample testing, such as blood sample testing.

[0023] In sample testing, different test items use different reagents, and a test often uses multiple different reagents. For example, in the testing of blood samples, the reagents used generally include diluents, sheath fluid reagents, immunoturbidimetric reagents, and immunochromatographic reagents. Diluents are used for pretreatment of blood samples to reduce certain high-concentration substances in the blood sample to an appropriate range to avoid high-concentration substances from being difficult to accurately detect; sheath fluid reagents are used to focus the blood sample to form a sample flow of appropriate length and stability, mainly for white blood cell counting; immunoturbidimetric reagents and immunochromatographic reagents are used for antigen-antibody reactions in blood samples, mainly for the detection of markers such as CRP, SAA, PCT, and IL-6. It should be understood that the above are just a few commonly used reagents, and the reagents used in sample testing are not limited to these.

[0024] Figure 1FIG. 1 is a schematic diagram illustrating a specific embodiment of a reagent filling device according to the present invention. The device includes a reagent container 20, a metering pump 22 connected to the reagent container 20, a first pressure source 24 for providing power to the metering pump 22, a first liquid reservoir 26, and a second liquid reservoir 28. Each component can be connected via pipelines, such as flexible hoses. The metering pump 22, the first pressure source 24, and the associated pipelines together constitute a liquid supply power unit that draws the reagent from the reagent container 20 and pours it into the first liquid reservoir 26 or the second liquid reservoir 28. The first pressure source 24 can be a power source that provides pressure, such as an air source, an air pump, or a syringe. In the figure, the first pressure source 24 is an air source, including a positive pressure air source 24a and a negative pressure air source 24b. The negative pressure air source 24b is used to provide a negative pressure P2 to enable the metering pump 22 to draw the reagent from the reagent container 20, while the positive pressure air source 24a is used to provide a positive pressure P1 to enable the metering pump 22 to pour the drawn reagent into the first liquid reservoir 26 or the second liquid reservoir 28.

[0025] The reagent container 20 is used to store the reagents needed for detection. It has a large volume and multiple dosages and is usually located outside the sample analyzer. The first liquid reservoir 26 and the second liquid reservoir 28 are located inside the sample analyzer. Their volumes are reduced by orders of magnitude relative to the reagent container 20, typically only 5 to 10 ml. The reagent container 20 is schematically represented by a box in the figure, and the shape, size, etc. of each component in the figure are not limited to the actual object. Usually, the metering pump 22 is located near the first liquid reservoir 26 and the second liquid reservoir 28. The length of the connecting pipeline between the metering pump 22 and the external reagent container 20 is much greater than the length of the connecting pipeline between the metering pump 22 and the internal first liquid reservoir 26 and the second liquid reservoir 28. In this way, during the sample detection process, the detection module obtains reagents from the first liquid reservoir 26 and the second liquid reservoir 28, which can significantly reduce the length of the reagent flow path and accelerate the process of sample detection.

[0026] In the illustrated embodiment, the positive pressure gas source 24a and the negative pressure gas source 24b are connected to the metering pump 22 via a first switching element 30. The first switching element 30 may be a three-way solenoid valve, etc., and includes a first interface A, a second interface B, and a third interface C. The first interface A is connected to the negative pressure gas source 24b, the second interface B is connected to the positive pressure gas source 24a, and the third interface C is connected to the metering pump 22. When the first switching element 30 is closed, its interfaces A and C are connected, and interfaces B and C are disconnected, connecting the negative pressure gas source 24b to the metering pump 22 so that the metering pump 22 can draw reagent from the reagent container 20. Conversely, when the first switching element 30 is opened, its interfaces B and C are connected, and interfaces A and C are disconnected, connecting the positive pressure gas source 24a to the metering pump 22 so that the metering pump 22 can pour the drawn reagent into the first and second liquid storage tanks 26 and 28.

[0027] Before performing a sample test, a sample analyzer employing the reagent filling device of the present invention uses a metering pump 22 to fill reagent from a first reagent container 20 into first and second liquid storage tanks 26 and 28 under the influence of positive and negative air pressure from a first pressure source 24. Thereafter, during the sample test, the detection module directly draws reagent from the first and second liquid storage tanks 26 and 28. In the above embodiment, metering pump 22 selectively connects positive and negative pressure sources 24a and 24b via a three-way solenoid valve, creating a linkage between positive and negative pressure sources 24a and 24b, simplifying operation. It should be understood that the first switching component 30 can also be composed of two two-way valves and a three-way connector, one interface of the three-way connector is connected to the positive pressure air source 24a through a two-way valve, one interface is connected to the negative pressure air source 24b through another two-way valve, and one interface is connected to the metering pump 22. It can also switch the connection between the metering pump 22 and the positive pressure air source 24a and the negative pressure air source 24b, so that the metering pump 22 can absorb the reagent from the reagent container 20 or inject the reagent into the first and second liquid storage tanks 26 and 28.

[0028] In this embodiment, the first liquid storage tank 26 and the second liquid storage tank 28 are arranged in parallel and connected to the metering pump 22 through a second switching member 32. The second switching member 32 is preferably a three-way solenoid valve, etc., including a first interface D, a second interface E and a third interface F, wherein the first interface D is connected to the second liquid storage tank 28, the second interface E is connected to the first liquid storage tank 26, and the third interface F is connected to the metering pump 22. The metering pump 22 selectively connects the first liquid storage tank 26 and the second liquid storage tank 28 through the second switching member 32. When the second switching member 32 is closed, its interfaces F and E are connected, and interfaces F and D are disconnected, connecting the metering pump 22 and the first liquid storage tank 26 so that the metering pump 22 can perfuse the reagent into the first liquid storage tank 26; conversely, when the second switching member 32 is opened, its interfaces F and D are connected, and interfaces F and E are disconnected, connecting the metering pump 22 and the second liquid storage tank 28 so that the metering pump 22 can perfuse the reagent into the second liquid storage tank 28.

[0029] In this embodiment, the second port B of the second switching element 32, the reagent container 20, and the first liquid reservoir 26 are connected via a tee joint 34. A first on / off member 36 is provided on the connecting pipeline between the tee joint 34 and the first liquid reservoir 26, and a second on / off member 38, such as a one-way valve, is provided on the connecting pipeline between the tee joint 34 and the reagent container 20. The first on / off member 36 can be a two-way solenoid valve that disconnects the tee joint 34 from the first liquid reservoir 26 when closed and connects the tee joint 34 and the first liquid reservoir 26 when opened. The second on / off member 38 maintains the flow path from the reagent container 20 to the tee joint 34 and disconnects the flow path from the tee joint 34 to the reagent container 20. That is to say, when the second switching member 32 is closed, if the first on-off member 36 is closed, the connection between the three-way joint 34 and the first liquid storage tank 26 is disconnected. At this time, only the flow path between the metering pump 22 and the reagent container 20 is connected, and the reagent flows to the metering pump 22; if the first on-off member 36 is opened, the three-way joint 34 and the first liquid storage tank 26 are connected. Under the unidirectional conduction effect of the second on-off member 38, the reagent can only flow from the metering pump 22 to the first liquid storage tank 26.

[0030] The first liquid storage tank 26 is used to temporarily store reagents with bubbles, and a first liquid outlet 27 is formed at its bottom for connection to the bubble-insensitive module of the sample analyzer; the second liquid storage tank 28 is used to temporarily store reagents without bubbles, and a second liquid outlet 29 is formed at its bottom for connection to the bubble-sensitive module of the sample analyzer. Preferably, the first liquid storage tank 26 is also connected to a second pressure source 40, which can be an air source, etc. In the process of perfusing the reagent into the first liquid storage tank 26, the air source 40 provides a negative pressure P3 to accelerate the perfusion of the reagent by the metering pump 22 into the first liquid storage tank 26; in the process of transporting the reagent outward from the first liquid storage tank 26, the air source 40 provides a positive pressure P4 to accelerate the output of the reagent. Preferably, a third switching component 42 is also connected between the second pressure source 40 and the first liquid storage tank 26, and the third switching component 42 can be a three-way solenoid valve, etc., which selectively connects the positive pressure P4 or the negative pressure P3 to the first liquid storage tank 26.

[0031] It should be noted that the bubble-sensitive module in the present invention refers to a module in which bubbles in the reagent will affect the processing results of the reagent by the module, such as the flow chamber, impedance post-cell and other detection modules of the sample analyzer, and the second liquid storage tank 28 provides reagents for reaction, detection or cleaning; the bubble-insensitive module refers to a module in which bubbles in the reagent will not affect the processing results of the reagent by the module, such as the reaction pool, swab, etc. of the sample analyzer, and the first liquid storage tank 26 provides reagents for cleaning.

[0032] When adding reagents, the present invention first fills a certain amount of reagent into the first liquid reservoir 26. During the filling process, the front-end air, including the air inside the metering pump 22 and the air in the connecting pipe between the metering pump 22 and the reagent container 20, is discharged into the first liquid reservoir 26, resulting in the reagent in the first liquid reservoir 26 containing small bubbles. After the front-end air is completely discharged, the reagent is then poured into the second liquid reservoir 28 to ensure that the reagent in the second liquid reservoir 28 is free of small bubbles. It should be understood that the "no small bubbles" in the reagent in the second liquid reservoir 28 described in the present invention does not mean that there are absolutely no bubbles, but rather that the bubble content is so low that it does not affect the accuracy of sample testing. This is because the reagent itself will contain a small amount of air, and the connecting pipe between the metering pump 22 and the second liquid reservoir 28 will also contain a very small amount of air, but this amount is negligible compared to the air in the connecting pipe between the metering pump 22 and the reagent container 20.

[0033] Figure 2 The second embodiment of the reagent filling device of the present invention is shown, which differs from the first embodiment mainly in the connection of the reagent container 20. In this embodiment, the reagent container 20 is connected between the metering pump 22 and the second switching member 32, and the pipelines between the three can be connected by a three-way joint 34. Specifically, the first interface D of the second switching member 32 is directly connected to the second liquid storage tank 28, the second interface E is connected to the first liquid storage tank 26 through the first switch 36, and the third interface F is connected to the three-way joint 34. When the first switching member 30 is closed, the second switching member 32 and the first switch 36 are closed, and the metering pump 22 draws reagent from the reagent container 20. When the first switching member 30 is opened, the first switch 36 is first opened, and the metering pump 22 fills the reagent into the first liquid storage tank 26; thereafter, the first switch 36 is closed and the second switching member 32 is opened, and the metering pump 22 fills the reagent into the second liquid storage tank 28, and the discharge of the front air to the first liquid storage tank 26 is also achieved, ensuring that the reagent in the second liquid storage tank 28 does not contain bubbles. In other embodiments, the reagent container 20 may be connected to the pipeline between the metering pump 22 and the second liquid storage tank 28 via a three-way connector 34 , as long as it can communicate with the metering pump 22 .

[0034] Figure 3The third embodiment of the reagent filling device of the present invention is shown, which differs from the second embodiment mainly in the second switching member. In this embodiment, the second switching member is composed of a first two-way valve 361, a second two-way valve 363, and a three-way connector 365, wherein the three-way connector 365 is connected in series between the metering pump 22 and the first two-way valve 361 and the second two-way valve 363, the first liquid storage tank 26 is connected to the first two-way valve 361, and the second liquid storage tank 28 is connected to the second two-way valve 363. The reagent container 20 is connected between the metering pump 22 and the three-way connector 365. When the first switching member 30 is closed, the first two-way valve 361 and the second two-way valve 363 are closed, and the metering pump 22 draws reagent from the reagent container 20. When the first switching member 30 is opened, the first two-way valve 361 is first opened, and the metering pump 22 injects the reagent into the first liquid storage tank 26; then, the first two-way valve 361 is closed and the second two-way valve 363 is opened, and the metering pump 22 injects the reagent into the second liquid storage tank 28, which also realizes the discharge of the front air into the first liquid storage tank 26, ensuring that the reagent in the second liquid storage tank 28 does not contain bubbles.

[0035] The present invention provides two liquid storage tanks 26 and 28 and successively fills the two liquid storage tanks 26 and 28 with reagents. In the process of filling the first liquid storage tank 26 with reagents, the air in the connecting pipe between the metering pump 22 and the reagent container 20 is emptied, and the reagent is poured into the second liquid storage tank 28 after the front air is exhausted. During the sample test, the second liquid storage tank 28 outputs the bubble-free reagent to the reaction and detection modules of the sample analyzer, etc., to ensure that the subsequent test is not affected by the bubbles and the accuracy of the test results is guaranteed; before or after the sample test is completed, the bubble-containing reagent in the first liquid storage tank 26, such as the diluent, can be output to clean the connecting pipes of the sample analyzer, etc., to prevent the residual sample liquid from affecting the accuracy of the test. Of course, if the reagent is not suitable for cleaning the pipe, it can be directly discharged into the waste liquid storage module of the sample analyzer.

[0036] The reagent filling method of the present invention mainly includes the following steps: first, the reagent is poured into the first liquid storage tank 26 to exhaust, that is, the front air, including the air inside the metering pump 22 and the air in the connecting pipe between the metering pump 22 and the reagent container 20, is discharged into the first liquid storage tank 26; secondly, the reagent is poured into the second liquid storage tank 28, that is, the reagent is poured into the second liquid storage tank 28 for temporary storage after the front air is exhausted.

[0037] Specifically, the process of filling the first liquid reservoir 26 with reagent to expel air includes the following steps: 1) closing the first switching element 30 to connect the negative pressure air source 24b to the metering pump 22, and closing the second switching element 32 to connect the metering pump 22 to the reagent container 20. The metering pump 22 draws the reagent from the reagent container 20 under the action of negative pressure P1. 2) opening the first switching element 30 to connect the positive pressure air source 24a to the metering pump 22, and opening the first on / off element 36 to connect the metering pump 22 to the first liquid reservoir 26. The metering pump 22, under the action of positive pressure P2, pours the drawn reagent into the first liquid reservoir 26. During the process of the metering pump 22 drawing the reagent and pouring it into the first liquid reservoir 26, the front-end air is expelled into the first liquid reservoir 26 along with the reagent. To ensure that the front-end air is completely expelled, steps S11 and S12 can be repeated multiple times, for example, 3 to 5 times.

[0038] Specifically, the process of perfusing the reagent into the second liquid storage tank 28 includes the following steps: 1) closing the first switching member 30 to connect the negative pressure gas source 24b and the metering pump 22, closing the second switching member 32 to connect the metering pump 22 and the reagent container 20, and the metering pump 22 draws the reagent from the reagent container 20 under the action of the negative pressure P1; 2) opening the first switching member 30 to connect the positive pressure gas source 24a and the metering pump 22, opening the second switching member 32 to connect the metering pump 22 and the second liquid storage tank 28, and the metering pump 22 pours the drawn reagent into the second liquid storage tank 28 under the action of the positive pressure P2. Depending on the specific content of the sample test, the amount of reagent used can be estimated. Based on the amount of reagent used, the step of perfusing the reagent into the second liquid storage tank 28 by the metering pump 22 can be performed once or repeatedly until the reagent injected into the second liquid storage tank 28 meets the needs of the sample test or the second liquid storage tank 28 is full.

[0039] In the above embodiment, the metering pump 22 is combined with the first pressure source 20 to form a liquid supply power device, which can better control the amount of reagent injected into the liquid storage tanks 26 and 28. In some embodiments, the liquid supply power device can be composed of a motor and a syringe, the syringes are respectively connected to the first liquid storage tank and the second liquid storage tank, and the motor serves as a power source to drive the syringes to aspirate or inject liquid; or, the liquid supply power device includes a pressure source and a liquid reservoir; or, the liquid supply power device is composed of a syringe alone, and the aspiration or injection operation is performed manually by the user; or, the liquid supply power device can be a combination of a pressure source and a liquid reservoir, the liquid reservoirs are respectively connected to the first liquid storage tank and the second liquid storage tank, and the pressure source is connected to the liquid reservoir via a switching element, without being limited to the specific embodiment. The metering pump, syringe, liquid reservoir, etc. constitute the storage structure of the liquid supply power device, which can temporarily store the aspirated reagent; the pressure source, motor, syringe, etc. constitute the power source of the liquid supply power device, aspirating the reagent in the reagent container 20 into the storage structure and then injecting it into the first liquid storage tank 26 and the second liquid storage tank 28.

[0040] The reagent filling method of the present invention sequentially fills the first and second liquid storage tanks 26 and 28 with reagents by setting a filling sequence. The reagents and the front air sucked by the metering pump 22 for the first few times are injected into the first liquid storage tank 26. After the front air is exhausted, the reagents sucked by the metering pump 22 are injected into the second liquid storage tank 28. It is ensured that the reagent injected into the second liquid storage tank 28 does not contain bubbles. The accuracy of the test results can be ensured when the quantitative reaction of the sample detection is applied later. The reagent containing the front air in the first liquid storage tank 26 can be used to clean the connecting pipes, etc., so as to make the best use of the reagents and avoid waste. The present invention uses two liquid storage tanks 26 and 28 to store the reagent containing bubbles and the reagent not containing bubbles respectively. The structure is simple and easy. During the reagent filling process, the flow path of the two liquid storage tanks 26 and 28 is controlled to be on and off to realize the sequential filling of the two liquid storage tanks 26 and 28, and complete the separation of the reagent containing bubbles. The operation is simple and convenient, the bubble separation effect is good, and the reliability of the sample detection is ensured.

[0041] It should be noted that the present invention is not limited to the above-mentioned embodiments. Based on the creative spirit of the present invention, those skilled in the art can also make other changes. These changes made based on the creative spirit of the present invention should be included in the scope of protection required by the present invention.

Claims

1. A reagent filling device, characterized in that: The system comprises a liquid supply power device and a first liquid storage tank and a second liquid storage tank connected to the liquid supply power device, wherein the first liquid storage tank and the second liquid storage tank are arranged in parallel and selectively connected to the liquid supply power device. The liquid supply power device first fills a certain amount of reagent into the first liquid storage tank to discharge the air in the liquid supply power device into the first liquid storage tank, and then fills the second liquid storage tank with reagent. The first liquid storage tank is used to provide reagent to the bubble-insensitive module, and the second liquid storage tank is used to provide reagent to the bubble-sensitive module. The liquid supply power device includes a first pressure source and a metering pump, the first pressure source is connected to the metering pump through a first switching member, and the metering pump is connected to the first liquid storage tank and the second liquid storage tank respectively; The metering pump is connected to the first liquid storage tank and the second liquid storage tank via a second switching member; Also included is a reagent container, which is connected to the metering pump via a one-way valve; The reagent container is connected between the second switching member and the first liquid storage tank; The reagent container is connected to the first liquid storage tank via the one-way valve, and a switch is connected between the reagent container and the first liquid storage tank; The first liquid storage tank is also connected to a second pressure source; A third switching element is connected between the second pressure source and the first liquid storage tank.

2. A reagent filling method, using the reagent filling device according to claim 1, comprising the following steps: S1, connecting the liquid supply power device to the first liquid storage tank and disconnecting the liquid supply power device from the second liquid storage tank, and filling the first liquid storage tank with reagent until the air in the liquid supply power device is emptied; S2, connecting the liquid supply power device and the second liquid storage tank and disconnecting the liquid supply power device and the first liquid storage tank, and filling the second liquid storage tank with reagent.

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