An alkali-resistant microorganism, microbial agent and their use in the extraction of silicon iron from volcanic rock

Abstract

The present invention relates to an alkali-tolerant microorganism, a microbial agent and their use in the extraction of volcanic rock ferrosilicon. The name is Paenibacillus simplex WS-L19 ( Peribacillus simplex WS-L19), which is deposited in the China Center for Type Culture Collection with the deposit number of CCTCC NO: M 2022767. The present invention can be used in environmental protection, mineral smelting and metal leaching technologies. The alkali-tolerant microorganism has the advantages of easy cultivation and short growth cycle, and can grow under neutral and alkaline conditions.

Description

Technical Field

[0001] The present invention belongs to the field of biotechnology, and particularly relates to an alkali-tolerant microorganism, a microbial agent and their use in the extraction of silicon iron from volcanic rocks. Background Art

[0002] Mineral weathering is the basis for various landform forms and soil formation on the earth's surface. The weathering of minerals by animals, plants and microorganisms is one of the most important components. Weathering refers to the process in which the rock structure and composition are mechanically broken and then cracked to form small particles or dissolved to form biogenic minerals under physical, chemical or biological factors, and it is the most important geochemical process occurring on the earth's surface. Rock weathering can be divided into physical weathering, chemical weathering and biological weathering according to the causes. Physical weathering includes the weathering of rocks caused by factors such as crustal movement, temperature change, rain erosion, freezing, etc., which refers to the mechanical fragmentation of rocks on the earth's surface without changing their chemical composition and without generating new minerals. Chemical weathering refers to the action of H2O, O2, CO2, organic substances and various cations and anions on the earth's rock surface, resulting in changes in chemical composition and mineral composition and generating new minerals. Biological weathering refers to the phenomenon that the rock surface undergoes mechanical damage or biological corrosion to produce cracks or corrosion under the influence of the growth, development and reproduction of organisms or the by-products of their secreted metabolism. The interaction between minerals and microorganisms is of great significance to rock weathering, the geochemical cycle of geological elements and the environment.

[0003] Microorganisms can accelerate the dissolution of minerals through their own growth and metabolic processes and some extracellular substances secreted. Microbial weathering is an important aspect of biological weathering. The role of microorganisms in the weathering of rocks or single minerals is that the activities of microorganisms can cause changes in the composition of different types of rocks or minerals, and the changed components are mainly some important elements such as Si, Al, Fe, Mg, Ca, etc. The minerals formed by microbial induction are mainly by-products in the physiological metabolism process of microorganisms. Since the activities of microorganisms change the microenvironment for mineral formation, it promotes the formation of minerals. In addition, bacterial mineral weathering is used in bioleaching in industry. It can use the extracellular polysaccharide substances secreted by bacteria to corrode metals, so that they can be biologically leached, and finally precious metals can be obtained.

[0004] Metal leaching is a new biotechnology for metal extraction and recovery. Iron and silicon are essential elements in industrial production. Bacterial leaching is a leaching method that uses microorganisms and their metabolites to oxidize and leach the target components in ores. However, the existing metal leaching technologies still have disadvantages such as low leaching rate, long growth cycle, complex culture conditions, and difficult-to-obtain culture raw materials. The microorganisms used for metal leaching by bacterial leaching are generally acidophilic microorganisms, which are not suitable for metal leaching in alkaline environments. Summary of the Invention

[0005] In view of the problems existing in the prior art, the present invention provides an alkali-tolerant microorganism, a bacterial agent and their use in the leaching of volcanic rock silicon iron, which have the advantages of being suitable for metal leaching in alkaline environments, high leaching rate, low cost, short growth cycle, simple process culture conditions, easy availability of raw materials, and being beneficial to environmental protection.

[0006] The technical solutions of the present invention to solve the above technical problems are as follows:

[0007] The present invention provides an alkali-tolerant microorganism named Peribacillus simplex WS-L19, with a preservation number of CCTCC NO: M 2022767, a preservation time of May 31, 2022, and is currently preserved in the China Center for Type Culture Collection. The address is: Wuhan University, Wuhan, China.

[0008] The alkali-tolerant microorganism provided by the present invention is a strain cultured and isolated from a volcanic rock sample of Weishan in Wudalianchi, Heihe City, Heilongjiang Province and obtained through screening and subculture. The cells are rod-shaped, alkali-tolerant, Gram-positive, with a size of 2.5-3.0 μm × 0.6-0.8 μm, and the surface is smooth. It can be used for metal leaching under alkaline conditions, and this technology provides a new method for the leaching of volcanic rock silicon iron.

[0009] Compared with the prior art, the microorganism provided by the present invention has better alkali tolerance and can grow in alkaline environments. Moreover, the process culture conditions are simple, the raw materials are easy to obtain, and it is beneficial to environmental protection. Compared with traditional smelting technologies, smelting using the microorganism provided by the present invention has the advantages of low cost and little environmental pollution.

[0010] The present invention provides a bacterial agent, which includes the above alkali-tolerant microorganism and / or the fermentation broth of the above alkali-tolerant microorganism. In the bacterial agent, in addition to the alkali-tolerant microorganism and / or the fermentation broth of the above alkali-tolerant microorganism, other substances can also be added. The other substances can be substances that are beneficial for preservation and utilization or other strains for compounding, etc.

[0011] The microbial agent of the present invention is a mesophilic bacterium that can grow within the range of 20 - 40 °C, with a suitable growth temperature of 30 - 35 °C and an optimal growth temperature of 35 °C. It can grow under neutral and alkaline conditions, with a suitable growth pH of 6.00 - 11.00 and an optimal growth pH of 7.00, and the culture conditions are simple. The produced siderophore can specifically bind to ferric iron through the siderophore in a low-Fe environment, thereby improving the absorption and utilization efficiency of Fe.

[0012] Compared with similar metal-leaching microorganisms, the present invention can avoid the problem of difficult silicon and iron leaching in an alkaline environment, and this microbial agent is obtained by natural enrichment, having the advantage of good stability. Moreover, the microbial agent provided by the present invention has simple culture conditions and high leaching efficiency, and can be industrially produced.

[0013] The present invention provides a fermentation method for the above-mentioned alkali-tolerant microorganism, comprising the following steps: inoculating the above-mentioned alkali-tolerant microorganism into a culture medium and performing fermentation culture.

[0014] The culture medium can be LB medium, and the pH of the fermentation culture is 5.00 - 11.00 (preferably, the pH is 6.00 - 11.00; most preferably, the pH is 7.00), and the fermentation culture temperature is 20 - 40 °C (preferably, the suitable growth culture temperature is about 35 °C, and the suitable extraction culture temperature is 36.6 °C).

[0015] Adopting the above conditions is beneficial to the growth of the above-mentioned alkali-tolerant microorganism.

[0016] The present invention provides the application of the above-mentioned alkali-tolerant microorganism in mineral smelting or metal extraction.

[0017] Furthermore, it is used for the smelting or metal extraction of a combination of one or more of silicon, aluminum, iron, magnesium, and calcium.

[0018] For example: The present invention provides the use of the above-mentioned Peribacillus simplex WS-L19 in the extraction of silicon and iron from volcanic rocks, which is convenient for extracting minerals from volcanic rocks and provides a new idea for mineral extraction.

[0019] The present invention provides the application of the above-mentioned microbial agent in mineral smelting or metal extraction.

[0020] Furthermore, it is used for the smelting or metal extraction of a combination of one or more of silicon, aluminum, iron, magnesium, and calcium.

[0021] The microorganism or microbial agent provided by the present invention can be applicable to alkaline conditions and has the advantages of high leaching rate, good stability, low cost, short growth cycle, simple process culture conditions, easily available raw materials, and being beneficial to environmental protection.

[0022] The present invention provides a method for smelting minerals or leaching metals, comprising the following steps: using the above-mentioned alkali-resistant microorganisms or the above-mentioned microbial agents to smelt minerals or leach metals.

[0023] Furthermore, one or a combination of several of silicon, aluminum, iron, magnesium, and calcium is obtained through mineral smelting or metal leaching.

[0024] Furthermore, the above-mentioned alkali-resistant microorganisms or the above-mentioned microbial agents are inoculated into a culture medium, and volcanic rocks are added to the culture medium for fermentation culture.

[0025] For example: during use, the volcanic rocks are fermented and cultured in the culture medium of the above-mentioned microorganisms for leaching. The leaching of silicon and iron from volcanic rocks can be carried out according to the following steps.

[0026] Preferably, the optimized leaching conditions can be: the initial pH value of the culture medium is 6.00 - 9.00 (preferably, the initial pH of the culture medium is 7.00), the culture temperature is 20 - 40 °C (preferably, the fermentation culture temperature is 30 - 40 °C), the fermentation culture time is 6 - 9 d, the inoculation amount is 1% (v / v), the addition amount of volcanic rocks is 2% (w / v), and the liquid loading amount of the system is 50% (v / v).

[0027] Adopting the above conditions is beneficial to the ability of the microorganisms to produce acid, produce siderophores, and form biofilms, providing conditions for eroding volcanic rocks. Description of the Drawings

[0028] Figure 1 It is a Gram staining diagram of Peribacillus simplex WS-L19.

[0029] Figure 2 It is a scanning electron microscope diagram of volcanic rocks before and after inoculation with bacteria (Note: a is the electron microscope picture of volcanic rocks; b is the electron microscope picture of volcanic rocks under the erosion of Peribacillus simplex WS-L19).

[0030] Figure 3 It is a phylogenetic tree diagram of Peribacillus simplex WS-L19. Detailed Embodiments

[0031] Unless otherwise defined, all the technical terms used hereinafter have the same meaning as commonly understood by those skilled in the art.

[0032] The technical terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the protection scope of the present invention.

[0033] Unless otherwise specifically stated, all kinds of raw materials, reagents, instruments, and equipment used in the present invention can be obtained through market purchase or can be prepared by existing methods.

[0034] The present invention is not limited to the specific embodiments or examples listed below, and also includes any combination between the specific embodiments or examples.

[0035] The present invention overcomes the constraints in the field of bacterial leaching under alkaline conditions and provides an alkali-tolerant microorganism.

[0036] This alkali-tolerant microorganism was collected from volcanic rock samples of Zhongwei Mountain in the Wudalianchi volcanic group. Using a combination of traditional separation techniques and molecular identification, 19 culturable microorganisms were enriched and isolated. Volcanic rocks with a size of about 2 mm obtained by sieving were used as the test rocks. 50 mL of 10% LB medium was dispensed into 100 mL Erlenmeyer flasks, 0.5 g of volcanic rock was added, and sterilized at 120 °C for 20 min. The obtained weathered bacteria were respectively inoculated into 50 mL of the medium and cultured for 12 - 24 h. After centrifugation (8000 rpm, 2 min) to collect the bacterial cells, they were washed 3 times with physiological saline and then resuspended in physiological saline. The OD 600 was adjusted to about 0.6. 1 mL of the seed solution was inoculated into a 100 mL Erlenmeyer flask, and the non-inoculated treatment was used as a control. At the same time, 3 replicates were set up and cultured in an orbital shaker incubator at 30 °C and 120 rpm for 3 d. The increase in the contents of Fe and Si in the fermentation broth was used as a marker for volcanic rock erosion. The WS-L19 strain was obtained. The contents of Fe and Si in the WS-L19 culture broth were significantly higher than those of other culturable bacteria, reaching 14.06 ± 0.10 mg·L -1 and 146.11 ± 3.75 mg·L -1 , respectively. The results of the volcanic rock erosion test showed that the microorganism numbered WS-L19 had the best results.

[0037] This microorganism was identified as Peribacillus simplex WS-L19 and has the following characteristics: The bacterial cells are rod-shaped and Gram-positive ( Figure 1), with dimensions of 2.5 - 3.0 μm × 0.6 - 0.8 μm; it has a strong acid-producing ability and presents light yellow round colonies on 10% LB solid medium, with a colony diameter of 0.3 - 0.8 mm and a smooth surface. Peribacillus simplex WS-L19 has strong alkalinity tolerance and can grow normally within the range of pH 6.00 - 11.00, with the optimal growth at pH 7.00. Peribacillus simplex WS-L19 is a mesophilic bacterium that can grow within the range of 20 - 40 °C, with a suitable growth temperature of 30 - 35 °C and an optimal growth temperature of about 35 °C. The suitable temperature for leaching is about 36.6 °C. It can grow normally within the range of salinity of 0.05 - 3% and has good salt tolerance. It is found that Peribacillus simplex WS-L19 has the abilities of acid production, siderophore production and biofilm formation, providing conditions for the erosion of volcanic rocks.

[0038] The present invention provides a microbial agent, comprising the above-mentioned alkali-tolerant microorganism and / or the fermentation broth of the above-mentioned alkali-tolerant microorganism.

[0039] The present invention provides a method for smelting or leaching metals using the above-mentioned microorganism or microbial agent, comprising the following steps: placing volcanic rocks in a culture medium containing the above-mentioned microorganism or microbial agent to leach silicon, aluminum, iron, magnesium, and calcium.

[0040] The specific operation is as follows: inoculating the above-mentioned microorganism or microbial agent into a culture medium, adding volcanic rocks to the culture medium inoculated with the microorganism or microbial agent, and performing fermentation culture. In the initial stage of the erosion of volcanic rocks by Peribacillus simplex WS-L19, the pH of the fermentation system gradually increases, and OH - destroys the Si-O bond, promoting the bioweathering of volcanic rocks; subsequently, a large number of microorganisms proliferate and form a biofilm on the surface of volcanic rocks. In the microenvironment of the biofilm, a large amount of organic acids such as succinic acid, lactic acid, and acetic acid and siderophores produced by Peribacillus simplex WS-L19 dissolve elements such as Si, Fe, Al, Mg, and Ca in biotite, pyroxene, etc. through acidolysis and ligand complexation, thereby destroying the structure of volcanic rocks and promoting erosion ( Figure 2 ).

[0041] For example, the following method can be adopted: Add 2% (w / v) volcanic rock to the medium containing Peribacillus simplex WS-L19 for leaching. During leaching, the temperature of the medium is 36.6 °C, the concentration of the medium is 30.9% LB medium, and the initial pH value of the medium during leaching is 7.00; inoculate the obtained weathering bacteria into 50 mL of the medium respectively and culture for 12 - 24 h. After centrifuging (8000 rpm, 2 min) to collect the bacterial cells, wash them 3 times with physiological saline and then resuspend them in physiological saline, and adjust the OD 600 to about 0.3. Take 1 mL of the seed liquid and inoculate it into a triangular flask containing 100 mL of the medium (inoculation amount is 1%), the oscillation speed is 127.2 rpm / h, and the leaching process is 6 d. By adopting this method, the optimal conditions for eroding the volcanic rock are achieved, and the concentrations of Fe and Si in the culture solution are 85 μg·g -1 and 1005 μg·g -1 .

[0042] Adopting the above parameters is beneficial to accelerating the growth and reproduction rate of the bacterial cells and shortening the culture time.

[0043] In the specific implementation process, specific concentrations can be configured according to the following ratios.

[0044] The method for preparing the medium of the technical solution of the present invention includes the following steps:

[0045] LB medium: Prepared according to the following ratio, 10.0 g of sodium chloride, 10.0 g of tryptone, 5.0 g of yeast extract powder, 1 L of distilled water, adjust the pH to 7.0 ± 0.2 with 0.1 M sodium hydroxide solution, and sterilize at 121 °C for 20 min in a high-pressure steam sterilizer.

[0046] 10% LB medium: Prepared according to the following ratio, 100 mL of LB medium, 900 mL of distilled water, adjust the pH to 7.0 ± 0.2 with 0.1 M sodium hydroxide solution, and sterilize at 121 °C for 20 min in a high-pressure steam sterilizer.

[0047] Example 1 Isolation and Identification of Alkaline-Resistant Microorganisms

[0048] Collect volcanic rock samples from Weishan in Wudalianchi, Heilongjiang Province. Place 5 g of volcanic rock in 50 mL of sterile water, enrich at 120 rpm and 30 °C for 2 h, take out the enrichment solution as the original bacterial solution and dilute it 10 -1 and 10 -2 and 10 -3 times. Respectively absorb 100 μL and coat it on 10% LB solid medium and YN solid medium, and culture in a constant temperature incubator at 28 °C for 12 - 36 h. During this period, randomly pick 50 - 60 single colonies and streak and purify them more than 3 times to obtain 19 purified strains.

[0049] The purified strains were screened, and the screening method included the following steps: volcanic rocks of about 2 mm obtained by sieving were used as the test rocks, 10% LB medium was used as the medium for the volcanic rock erosion test, and the increase in the contents of Fe and Si in the fermentation broth was used as the sign of volcanic rock erosion. It was found that the contents of Fe and Si in the culture broth of the strain numbered WS-L19 were significantly higher than those of other culturable bacteria.

[0050] The strain WS-L19 was identified by 16S rRNA gene, and the identification method included the following steps: an appropriate amount of single colonies were picked and dissolved in 1 mL of ddH2O (double-distilled water), and bacterial DNA was extracted according to the steps in the DNA extraction kit instruction manual to obtain the bacterial DNA as the template for PCR amplification. The total PCR system was 25 μL, including 12.5 μL of 2×Taq PCR Master Mix, 0.5 μL of upstream primer 27F, 0.5 μL of downstream primer 1492R, 1 μL of DNA template added, and supplemented to 25 μL with ddH2O. The reaction conditions were: pre-denaturation at 95 °C for 5 min; denaturation at 95 °C for 30 s, annealing and renaturation at 52 °C for 30 s, extension at 72 °C for 60 s, for a total of 30 cycles; finally, extension at 72 °C for 5 min and preservation at 4 °C. 5.0 μL of the PCR amplification product was subjected to 1.0% agarose gel electrophoresis, and the PCR amplification product was detected under a gel cutting instrument. The PCR amplification product with clear bands conforming to the sample standard was sent to Bioengineering (Shanghai) Co., Ltd. for sequencing to obtain the product sequence. The product sequence was spliced using DNAMAN (version 8.0, USA), the obtained bacterial 16S rRNA gene sequence was aligned using EZBioCloud (https: / / www.ezbiocloud.net / identify), and a phylogenetic tree was constructed using the neighbor-joining method with the standard strain applied by MEGA X (https: / / www.megasoftware.net / ) software (such as Figure 3 ) to preliminarily determine the phylogenetic status of the isolated and purified weathering bacteria in microbial taxonomy.

[0051] Adopting the above identification method, it was identified that this microorganism belongs to the genus Peribacillus, and it was named Peribacillus simplex WS-L19. On May 31, 2022, Peribacillus simplex WS-L19 was deposited in the China Center for Type Culture Collection, with the deposit number CCTCC NO: M 2022767, and the address is: Wuhan University, Wuhan, China.

[0052] The inventors further studied, and the results showed that the erosion mechanism of Peribacillus simplex WS-L19 on volcanic rocks is mainly through acidolysis, alkalinolysis, biofilm action, siderophore action, etc. Hydrogen ions in organic acids such as succinic acid, propionic acid, and lactic acid produced by Peribacillus simplex WS-L19 may undergo ion exchange with the rock, and a large amount of elements such as Fe in the volcanic rock are dissolved out through the combination of biological action and acidolysis.

[0053] Example 2

[0054] The formula for culturing in LB medium includes: 10.0 g of sodium chloride, 10.0 g of tryptone, 5.0 g of yeast extract powder, 1 L of distilled water. Adjust the concentration of LB medium to 30.9% with distilled water, the inoculation amount is 1% (v / v), the loading volume is 50% (v / v), the addition amount of volcanic rock is 2% (w / v), the rotation speed is 127.2 rpm, the temperature is 36.6 °C, and the culture time is 6 d.

[0055] The initial pH values of the culture solution are set to 5.00, 6.00, 7.00, 8.00, and 9.00 respectively. Peribacillus simplex WS-L19 has a relatively wide pH growth range and can grow within the range of pH 5.00 - 9.00. When the initial pH is 7.00, the dissolution rates of iron and silicon are relatively high, which are 2.38% and 3.88% respectively.

[0056] Calculation method of iron dissolution rate: Content of iron in the leaching solution / Total iron content of volcanic rock × 100%.

[0057] Calculation method of silicon dissolution rate: Content of silicon in the leaching solution / Total silicon content of volcanic rock × 100%.

[0058] The results show that when the pH is 5.00, the growth of the strain is slow in the initial stage of erosion, indicating that the acidic environment will inhibit the growth of the strain; when the initial pH is 9.00, Peribacillus simplex WS-L19 still grows normally, indicating that the strain has strong alkalinity tolerance.

[0059] Example 3

[0060] The difference between this example and Example 2 is: the initial pH of the medium during leaching is 7.00, and the culture temperature is set to 20 - 40 °C, and other steps are the same as in Example 2.

[0061] Experimental results: The suitable temperature for the growth of the bacterial agent is 30 - 35 °C, and the optimal temperature for leaching is 36.6 °C.

[0062] Example 4

[0063] Inoculate the Peribacillus simplex WS-L19 bacterial solution (OD 600 is about 0.3) into 10% LB medium at an inoculation amount of 1% (v / v), and culture it under the conditions of pH 7.00, rotation speed of 127.2 rpm, temperature of 36.6 °C, liquid loading of 50% (v / v), and volcanic rock addition amount of 2% (w / v). Set the erosion time to 0 d, 3 d, 6 d, 9 d, 12 d, and 15 d.

[0064] The results show that when the erosion time is 0 - 9 d, the dissolution rates of Fe and Si in the volcanic rock gradually increase with time. After 9 d of erosion time, the dissolution rates of Fe and Si no longer increase, and the dissolution rates are 1.66% and 2.86% respectively.

[0065] Calculation method of iron dissolution rate: content of iron in the leaching solution / total iron content of the volcanic rock × 100%.

[0066] Calculation method of silicon dissolution rate: content of silicon in the leaching solution / total silicon content of the volcanic rock × 100%.

[0067] Example 5

[0068] Inoculate the Peribacillus simplex WS-L19 bacterial solution (OD 600 is about 0.3) into 30.9% LB medium, add 2% (w / v) of volcanic rock, and culture it for 6 d under the conditions of pH 7.00, rotation speed of 100 rpm, and temperature of 35 °C. Set the inoculation amount to 0.1% - 10% (v / v), liquid loading of 50% (v / v), and observe the erosion of the volcanic rock by Peribacillus simplex WS-L19.

[0069] As shown by the results, the erosion effect of Peribacillus simplex WS-L19 shows a trend of first increasing and then decreasing with the increase of the inoculation amount, and the dissolution rates of Fe and Si in the volcanic rock are the highest at an inoculation amount of 1% (v / v), which are 2.0% and 2.7% respectively.

[0070] Calculation method of iron dissolution rate: content of iron in the leaching solution / total iron content of the volcanic rock × 100%.

[0071] Calculation method of silicon dissolution rate: content of silicon in the leaching solution / total silicon content of the volcanic rock × 100%.

[0072] Example 6

[0073] The erosion conditions of Peribacillus simplex WS-L19 were optimized by the response surface method. There is a maximum predicted value on the response surface. When the rotation speed is fixed at 130.4 rpm, the temperature is 30.0 °C, and the concentration of LB medium is 38.2%, Peribacillus simplex WS-L19 has a better erosion effect on volcanic rock, the highest Fe dissolution amount, and the predicted Fe concentration in the fermentation broth can reach 85 μg·g -1 .

[0074] When the concentration of LB medium is fixed at 35.2%, the rotation speed is 125.6 rpm, and the temperature is 35.7 °C, Peribacillus simplex WS-L19 has a better erosion effect on volcanic rock, the highest Si dissolution amount, and the predicted Si concentration in the fermentation broth can reach 1045 μg·g -1 .

[0075] When the temperature is fixed at 36.6 °C, the rotation speed is 127.2 rpm, and the concentration of LB medium is 30.9%, the predicted satisfaction is as high as 97.3%. Peribacillus simplex WS-L19 has the best erosion effect on volcanic rock, and the dissolution amounts of Fe and Si obtained are 85 μg·g -1 、1005 μg·g -1 .

[0076] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. An alkali-tolerant microorganism, characterized in that, The name is Bacillus perennis simplex ( Peribacillus simplex ) WS-L19, and the preservation number is CCTCC NO: M 2022767.

2. A bacterial agent, characterized in that, Comprising the alkali-tolerant microorganism described in claim 1 and / or the fermentation broth of the alkali-tolerant microorganism described in claim 1.

3. A fermentation method of the alkali-resistant microorganism according to claim 1, characterized in that, Comprising the following steps: Inoculating the medium with the alkali-tolerant microorganism described in claim 1 and performing fermentation culture.

4. Use of the alkali-tolerant microorganism described in claim 1 in leaching silicon and / or iron.

5. Use of the microbial agent described in claim 2 in leaching silicon and / or iron.

6. A method for smelting or leaching metals from minerals, characterized in that, Comprising the following steps: Leaching silicon and / or iron using the alkali-tolerant microorganism described in claim 1 or the microbial agent described in claim 2.

7. The method according to claim 6, characterized in that, Inoculating the medium with the alkali-tolerant microorganism described in claim 1 or the microbial agent described in claim 2, adding volcanic rock to the medium, and performing fermentation culture.

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