Plant disease gas collection detection device and working method thereof

Abstract

The present application relates to the technical field of plant disease detection, and particularly relates to a plant disease gas collection and detection device and a working method thereof. The plant disease gas collection and detection device is provided with a stem and leaf part gas collection device and an animal detection box on a moving mechanism. The stem and leaf part gas collection device comprises a first mechanical arm, a gas collection cage for covering the stem and leaf part of a plant, a gas blowing pump, a gas blowing pipe and a first gas guide pipe. The animal detection box is fixed on the moving mechanism and has a movable chamber for accommodating animals. The first gas guide pipe is connected with the movable chamber. The device collects the gas containing volatile substances from the stem and leaf part of a plant and transmits the gas to the animal detection box, thereby solving the problem of inaccurate results caused by single sampling in the laboratory molecular identification technology. In combination with the stress response of animals with sensitive olfactory to the target odor source of the odor of infected plants, the condition of plant infection is determined, and the detection efficiency and accuracy of the Huanglongbing disease are greatly improved.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of plant disease detection, and particularly relates to a plant disease gas collection and detection device and a working method thereof. BACKGROUND

[0002] Citrus Huanglongbing (HLB) is a devastating disease transmitted by citrus psyllids and is a quarantine disease that spreads rapidly in citrus production areas. At present, HLB has broken out in various major citrus production areas in the world from time to time, causing heavy losses to citrus production. Citrus trees infected with HLB release a special odor that attracts psyllids, which feed and lay eggs on the HLB-infected trees, producing a large number of bacteria-carrying psyllids. Once the citrus tree is infected with the bacteria, the bacteria can proliferate in the body, and the infected tree will be infected for life, with accelerated development and improved reproductive capacity. If the prevention and control measures are not effective in the early stage of HLB outbreak, nearby plants that have not been infected will soon be infected, and the disease will spread rapidly, leading to the death of a large number of plants. Therefore, it is necessary to detect HLB in the early stage of outbreak and strengthen prevention and control measures.

[0003] At present, the methods for detecting HLB include field diagnosis, indicator crop identification, microscopic observation, serological identification, DNA-DNA hybridization identification and quantitative PCR detection. However, the field diagnosis method is easy to be confused with other disease symptoms, has strong subjectivity and low accuracy; the indicator crop identification method is time-consuming and is not conducive to rapid diagnosis; the microscopic observation method has uneven distribution of bacteria in the body of the diseased tree, inaccurate sampling and low detection rate; the antibody preparation technology in the serological identification method is complex, and the detection range is narrow; and the nucleic acid detection in the DNA-DNA hybridization identification method requires expensive test instruments and reagents.

[0004] Among them, the most reliable method for detecting HLB is the PCR detection method, which can quantitatively detect Candida species related to citrus HLB and is the most effective method for detecting HLB. However, this method needs to be completed in a laboratory, is expensive, has a long cycle and is complicated to operate, and is difficult to apply in actual production.

[0005] Citrus HLB is an infectious disease caused by a kind of bacterium (Candidatus Liberibacter asiaticus, CLas). After being cross-infected by citrus psyllids, CLas can systemically infect and exist in the plant body for a long time, and there is currently no plant body HLB pathogen killing measure that can be used in production. Gas chromatography-mass spectrometry analysis shows that the plants infected with CLas have a variety of unique volatile organic compounds compared with the uninfected plants, and research shows that this is mainly caused by the odor volatilized by the CLas pathogen itself.

[0006] In summary, in the process of detecting huanglongbing of citrus plants, how to design a gas collection detection device to improve the detection efficiency and accuracy of huanglongbing has become a technical problem to be solved by the technical personnel in the field. SUMMARY

[0007] The purpose of the present application is to provide a gas collection detection device for detecting huanglongbing of citrus plants, to improve the detection efficiency and accuracy of huanglongbing, to provide technical support for timely detection and removal of diseased plants, and to provide protection for sustainable development of agricultural economy.

[0008] To achieve the above purpose, the first scheme adopted by the present application is to provide a plant disease gas collection detection device, which comprises a moving mechanism, a stem and leaf part gas collection device and an animal detection box.

[0009] The moving mechanism carries the stem and leaf part gas collection device and the animal detection box.

[0010] The stem and leaf part gas collection device comprises a first mechanical arm, a gas collection cage for covering the plant stem and leaf part, a gas blowing pump, a gas blowing pipe and a first gas guide pipe. The base of the first mechanical arm is fixedly installed on the moving mechanism. The gas collection cage is installed at the extending end of the first mechanical arm. The gas collection cage is composed of a folding framework. The outer side of the folding framework is covered with a film. The gas blowing pipe is arranged along the extending direction of the folding framework. The leading end of the gas blowing pipe is connected with the gas blowing pump. The trailing end of the gas blowing pipe is provided with a gas outlet for blowing gas to the plant branch and leaf part. The gas inlet end of the first gas guide pipe is connected with the gas collection cage. The gas outlet end of the first gas guide pipe is connected with the animal detection box.

[0011] The animal detection box is fixed on the moving mechanism. The animal detection box has a movable chamber for accommodating animals. The first gas guide pipe is connected with the movable chamber.

[0012] As a preferred, the gas collection cage has an unfolded form and a folded form.

[0013] In the unfolded form, the folding framework is unfolded and the film is opened to form a cage structure, which can cover the plant stem and leaf part.

[0014] In the folded form, the folding framework is contracted and stored close to the first mechanical arm. In this way, all branches and leaves of the plant can be covered, so that the infection result can be obtained at the early stage of infection, and missed detection can be avoided.

[0015] As preferred, the folding framework comprises a top support, a side support and a bottom support connected in sequence, a connecting frame and a ball screw connected with the output end of the first mechanical arm, the top support, the side support and the bottom support constitute a framework unit, the framework unit is circularly arranged along the axis of the ball screw, and the top support is connected with the sliding nut of the ball screw through the connecting frame; the unfolding and folding states of the gas collection cage are switched by the power source. In this way, the ball screw is driven by the output end of the first mechanical arm, and then the rapid switching of the folding framework between the unfolding and folding states is realized, which is beneficial to further improve the detection efficiency of citrus Huanglongbing in the orchard.

[0016] As preferred, the moving mechanism is a unmanned aerial vehicle, a helicopter or a walking chassis; when the moving mechanism is a walking chassis, the moving mechanism also carries a root gas collection device; the root gas collection device comprises a second mechanical arm, a gas collection pile for extending into the soil of the plant roots, a probe device, a suction pump and a second gas pipe, the base of the second mechanical arm is fixedly installed on the walking chassis, the gas collection pile is installed at the extending end of the second mechanical arm, the probe device is embedded in the gas collection pile, and the probe device is connected with the animal detection box through the second gas pipe; the suction pump provides power to send the gas from the probe device to the animal detection box, and the end of the probe device is provided with a filter screen for forming a barrier to the soil. In this way, the unmanned aerial vehicle can be used on plants with long age and dense branches and leaves; the ground walking chassis can be used in areas with relatively large plant spacing, and the root infection can be detected at the same time.

[0017] As preferred, the gas outlet of the movable chamber is connected with a gas harmless treatment device. In this way, the leakage of the disease gas is reduced, the risk of attracting psyllids is reduced, and the transmission route of Huanglongbing through the detection process is cut off.

[0018] As preferred, the walking chassis is a tracked vehicle. In this way, the tracked vehicle can be more suitable for the road conditions in the orchard, and the flexibility of the walking chassis is further improved.

[0019] As preferred, the walking chassis is provided with an operation table for controlling the first mechanical arm and the second mechanical arm. In this way, the operation table is convenient for the detection personnel to control the first mechanical arm and the second mechanical arm on site.

[0020] As preferred, the animal detection box contains a rat for detecting gas. In this way, the rat's developed olfactory receptors are used to identify trace volatile organic compounds. By collecting volatile substances from the stem and leaf and root system of the plant, the target odor source of the infected plant odor is smelled by the well-trained rat, which will have a stress response. The odor of the normal plant is a non-target odor source, and the rat will not have any reaction when it smells it. By observing the animal's behavior, it can be determined whether the plant is infected with a disease.

[0021] To achieve the above-mentioned purpose, the second scheme adopted by the present application is to provide a working method of the plant disease gas collection and detection device as described above, comprising: moving the moving mechanism to the plant to be detected, the stem and leaf gas collection device covering the branches and leaves of the plant to be detected and collecting gas; the gas collected by the stem and leaf gas collection device is transported to the animal detection box, and whether the plant is infected with a disease is determined according to the reaction of the pre-trained animal.

[0022] The plant disease gas collection and detection device and its working method provided by the present application have the following outstanding substantial features and significant progress compared with the prior art: the plant disease gas collection and detection device collects the gas containing volatile substances from the stem and leaf and root system of the plant by using the stem and leaf gas collection device and the root gas collection device respectively, and transports the gas to the animal detection box. The gas collection cage is used to cover and absorb the volatile substances of the plant stem and leaf, and the gas collection pile is used to penetrate the soil to collect the volatile substances of the plant root system, realizing the detection of the whole plant, solving the problem of inaccurate results caused by single sampling of laboratory molecular identification technology, and combining the stress response of the olfactory sensitive animal to the target odor source of the infected plant odor to determine the infection of the plant. Disease condition, greatly improving the detection efficiency and accuracy of Huanglongbing disease, providing technical support for timely detection and removal of diseased plants, and providing guarantee for sustainable development of agricultural economy. BRIEF DESCRIPTION OF DRAWINGS

[0023] Figure 1 is a perspective structural schematic diagram of a plant disease gas collection and detection device in an embodiment of the present application;

[0024] Figure 2 is Figure 1 a perspective structural schematic diagram of a plant disease gas collection and detection device in another view of

[0025] Figure 3 is Figure 1 the front view of

[0026] Figure 4 is Figure 3 the top view of

[0027] Figure 5is a schematic diagram of the use state of the gas collection cage ready to unfold to form a cage cover on the plant stem and leaf part;

[0028] Figure 6 is a schematic diagram of the use state of the gas collection cage in the unfolded form;

[0029] Figure 7 is a schematic diagram of the three-dimensional structure of another plant disease gas collection and detection device in the embodiment of the application;

[0030] Figure 8 is Figure 7 a reference diagram of the use state of the plant disease gas collection and detection device.

[0031] The drawings show that: 1, walking chassis; 2, bearing platform; 3, stem and leaf part gas collection device; 4, root part gas collection device; 5, animal detection box; 6, operation table; 7, unmanned aerial vehicle; 8, landing gear; 31, first mechanical arm; 32, gas collection cage; 33, air blowing pump; 34, air blowing pipe; 35, first air guide pipe; 41, second mechanical arm; 42, gas collection pile; 43, air suction pump; 44, second air guide pipe; 321, top support; 322, side support; 323, bottom support; 324, connecting frame; 325, ball screw. DETAILED DESCRIPTION

[0032] The specific embodiments of the application will be described in detail below with reference to the accompanying drawings.

[0033] As Figures 1-8 shown, the plant disease gas collection and detection device in the embodiment of the application aims to improve the detection efficiency and accuracy of Huanglongbing.

[0034] The plant disease gas collection and detection device in the embodiment of the application can realize efficient and rapid plant detection through biological recognition, the gas collection cage can fully cover and absorb plant stem and leaf volatiles, the gas collection pile can penetrate the soil to collect plant root volatiles, realizing a full-body "physical examination" of the plant, and solving the problem of inaccurate results caused by single sampling of laboratory molecular identification technology. The plant disease gas collection and detection device in the embodiment of the application can be matched with an unmanned aerial vehicle, a camera and a display screen to realize remote control and 24-hour operation, which greatly saves manpower compared with traditional expert field diagnosis and one-by-one sampling identification, and is faster and better. It is particularly important that the application first proposes to use rats to detect plant diseases, and no pollutants and waste reagents are produced in the whole process, which not only ensures the accuracy of the detection results but also is more environmentally friendly and sustainable.

[0035] Example 1

[0036] [Plant disease gas collection and detection device-unmanned aerial vehicle type]

[0037] As Figure 7 shown, a plant disease gas collection detection device includes a unmanned aerial vehicle 7, a stem and leaf gas collection device 3 and an animal detection box 5. The unmanned aerial vehicle 7 carries the stem and leaf gas collection device 3 and the animal detection box 5 and moves to the vicinity of the plant. Among them, the unmanned aerial vehicle 7 realizes rapid take-off and landing through the landing gear 8.

[0038] As Figure 8 combined Figure 6 shown, the stem and leaf gas collection device 3 includes a first mechanical arm 31, a gas collection cage 32 for covering the stem and leaf of the plant, a gas blowing pump 33, a gas blowing pipe 34 and a first gas guide pipe 35. The base of the first mechanical arm 31 is fixedly installed on the walking chassis 1, the gas collection cage 32 is installed at the extension end of the first mechanical arm 31, the gas collection cage 32 is composed of a folding frame, the outer side of the gas collection cage 32 is covered with a film, the gas blowing pipe 34 is arranged along the extension direction of the folding frame, the leading end of the gas blowing pipe 34 is connected with the gas blowing pump 33, the trailing end of the gas blowing pipe 34 is provided with a gas outlet for blowing gas to the plant canopy, the gas inlet end of the first gas guide pipe 35 is connected with the gas collection cage 32, and the gas outlet end of the first gas guide pipe 35 is connected with the animal detection box 5.

[0039] The animal detection box 5 is fixed on the unmanned aerial vehicle 7, and the animal detection box 5 has a movable chamber for accommodating animals. The first gas guide pipe 35 is connected with the movable chamber.

[0040] The gas collection cage 32 has an unfolded state and a folded state. In the unfolded state, the folding frame is unfolded and the film is opened to form a cage structure, which can cover the stem and leaf of the plant; in the folded state, the folding frame is contracted and stored close to the first mechanical arm 31.

[0041] As Figure 6 shown, the folding frame includes a top support 321, a side support 322 and a bottom support 323 connected in sequence, a connecting frame 324 and a ball screw 325. The ball screw 325 is connected with the output end of the first mechanical arm 31. The top support 321, the side support 322 and the bottom support 323 constitute a frame unit, which is circularly arranged along the axis of the ball screw 325. The top support 321 is connected with the sliding nut of the ball screw 325 through the connecting frame 324. The unfolded state and the folded state of the gas collection cage 32 are switched by a power source.

[0042] The plant disease gas collection detection device provided in the embodiment of the application is used as follows: the unmanned aerial vehicle 7 takes off from the landing gear 8 and moves to the plant to be detected; the stem and leaf gas collection device covers the branches and leaves of the plant to be detected and collects gas; the gas collected by the stem and leaf gas collection device is transported to the animal detection box, and whether the plant is infected with disease is determined according to the reaction of the pre-trained animal. The unmanned aerial vehicle can also carry a camera, which is convenient for remote control by the detection personnel.

[0043] Example 2

[0044] [Plant Disease Gas Collection and Detection Device - Mobile Chassis Type]

[0045] like Figure 1 As shown, a plant disease gas collection and detection device includes a walking chassis 1, a stem and leaf gas collection device 3, a root gas collection device 4, and an animal detection box 5.

[0046] The top of the chassis 1 is equipped with a support platform 2 for housing the stem and leaf gas collection device 3, the root gas collection device 4, and the animal detection box 5. The chassis 1 is a tracked vehicle. This design makes the tracked vehicle more adaptable to the road conditions in the orchard, further enhancing the flexibility of the chassis 1.

[0047] like Figure 2 Combination Figure 6 The shown stem and leaf gas collection device 3 includes a first robotic arm 31, a gas collection cage 32 for covering the plant stems and leaves, an air pump 33, an air blowing pipe 34, and a first air guide pipe 35. The base of the first robotic arm 31 is fixedly mounted on the support platform 2. The gas collection cage 32 is installed at the output end of the first robotic arm 31.

[0048] The air collection cage 32 is constructed of a folded frame. A thin film covers the outside of the air collection cage 32. An air blowing pipe 34 is arranged along the extension direction of the folded frame. The first end of the air blowing pipe 34 is connected to an air pump 33. The tail end of the air blowing pipe 34 is provided with an air outlet for blowing air into the plant crown. The air inlet of the first air guide pipe 35 is connected to the air collection cage 32. The air outlet of the first air guide pipe 35 is connected to the animal detection box 5.

[0049] like Figure 2 Combination Figure 6 As shown, the root gas collection device 4 includes a second robotic arm 41, a gas collection pile 42 for extending into the plant roots, a probe, an air intake pump 43, and a second air delivery tube 44. The base of the second robotic arm 41 is fixedly mounted on the support platform 2. The gas collection pile 42 is installed at the output end of the second robotic arm 41. The probe is embedded in the gas collection pile 42. The probe is connected to the animal detection box 5 through the second air delivery tube 44. The air intake pump 43 provides power to send gas from the probe to the animal detection box 5. The end of the probe is equipped with a filter screen that forms a barrier against the soil.

[0050] like Figure 3 As shown, the animal detection box 5 is fixed on the support platform 2. The animal detection box 5 has an active chamber for accommodating animals. The first air guide tube 35 and the second air guide tube 44 are both connected to the active chamber.

[0051] like Figure 4As shown, the bearing platform 2 is provided with an operation table 6 for controlling the first mechanical arm 31 and the second mechanical arm 41. In this way, the operation table 6 facilitates the on-site control of the first mechanical arm 31 and the second mechanical arm 41 by the detection personnel.

[0052] Structure of the gas collection cage

[0053] As shown, Figure 5 In combination Figure 6 As shown, the gas collection cage 32 has an unfolded state and a folded state. In the unfolded state, the folding skeleton is unfolded and props open the film to form a cage structure, and the gas collection cage 32 encloses the plant stem and leaf part; in the folded state, the folding skeleton is retracted and is accommodated on the bearing platform 2 together with the first mechanical arm 31.

[0054] As shown, Figure 6 The folding skeleton includes a top support 321, a side support 322 and a bottom support 323 connected in sequence, and a connecting frame 324 and a ball screw 325. The ball screw 325 is connected to the output end of the first mechanical arm 31, and the top support 321, the side support 322 and the bottom support 323 form a skeleton unit. The skeleton unit is circularly arranged along the axis of the ball screw 325. The top support 321 is connected to the sliding nut of the ball screw 325 through the connecting frame 324.

[0055] In this way, the ball screw 325 is driven by the output end of the first mechanical arm 31, thereby realizing the rapid switching of the folding skeleton between the unfolded state and the folded state, which is conducive to further improving the detection efficiency of citrus Huanglongbing in the orchard.

[0056] The main function of the gas collection cage 32 is to collect the gas containing volatile substances in the stem and leaf part of the plant, which can ensure full coverage of the detection of all plant leaves. The folding gas collection cage is composed of multiple hard skeletons and a film, and the hard skeletons are hollow and sealed. When the gas collection and detection device is in standby state, the gas collection cage can be folded into a strip and placed on the tracked vehicle together with the first gas guide pipe; when working, the folding gas collection cage is opened, encloses the entire plant stem and leaf, and is inflated from the inside by the air blowing pump 33, and the gas from the plant stem and leaf part is blown from the bottom to the top through the air outlet after filtering dust, leaves and other solid substances through the filter screen, and then is transmitted to the animal detection box 5 through the first gas guide pipe 35.

[0057] Structure of the gas collection cage

[0058] The gas collection pile is mainly used for collecting volatile substances of plant roots. The gas collection pile 42 is mainly made of a telescopic hard pipe. In standby state, it can be retracted and placed on the tracked vehicle. In operation, the gas collection pile can be flexibly telescoped to explore different parts of the roots under the drive of the second mechanical arm. After determining the root collection point, the probe penetrates into the ground and expands when contacting the roots. After the probe expands, a plurality of filter screens are arranged inside to prevent soil from blocking the pipe. The root volatile substances are sucked into the transport pipe and transmitted to the animal detection box 5 through the suction pump 43.

[0059] Animal detection box

[0060] The animal detection box 5 can realize efficient and rapid plant detection through biological recognition. Taking rats as an example, the animal detection box 5 contains rats for detecting gas. In this way, the developed olfactory receptors of rats are used to identify trace volatile organic compounds. By collecting volatile substances from plant stems, leaves and root parts and transmitting them to the animal detection box 5, pre-trained rats can smell the target odor source of the infected plant odor, which will have a stress response. The odor of normal plants is a non-target odor source, and the rat will not have any reaction when it smells it. By observing the animal behavior, it can be determined whether the plant is infected with diseases.

[0061] Generally, rats have a short training cycle and low training cost, which is the best choice. Moreover, the olfactory sensitivity of rats is much higher than that of dogs. Similarly, by changing the structure of the animal detection box 5, for example, not using the animal detection box but using the gas guide outlet, it can be used to adapt to pre-trained pigs, dogs and other larger animals. It is also a common choice.

[0062] The gas outlet of the movable chamber is connected with a gas harmless treatment device. In this way, it is beneficial to reduce the leakage of disease gas and reduce the risk of attracting aphids, cutting off the transmission route of Huanglongbing through the detection process.

[0063] The animal detection box 5 can also be equipped with a complete set of animal training, living and working facilities, and equipped with a monitor. The monitor can transmit real-time images to the host computer. One side of the animal detection box 5 is also provided with a communication antenna for receiving unmanned aerial vehicle signals to realize remote control of the equipment.

[0064] The plant disease gas collecting and detecting device provided in the embodiment of the application is used, the stem and leaf part gas collecting device and the root part gas collecting device are used to collect the gas containing volatile substances of the stem and leaf and the root part of the plant, and the gas is transmitted to the animal detecting box, the gas collecting cage is used to absorb the volatile substances of the stem and leaf of the plant, the gas collecting pile is used to penetrate the soil to collect the volatile substances of the root of the plant, the whole body of the plant is detected, the plant with diseases can be found earlier, and more plants can be prevented from being infected in time. The device has shorter detection time, higher real-time performance, lower cost, is beneficial to actual production application, does not need to sample and damage the plant, and does not have the problems of inaccurate sampling and low detection rate.

Claims

1. A plant disease gas collection detection device, characterized by, The mobile mechanism, the stem and leaf gas collection device and the animal detection box are included. The mobile mechanism carries the stem and leaf gas collection device and the animal detection box. The stem and leaf gas collection device includes a first mechanical arm, a gas collection cage for covering the plant stem and leaf, a gas blowing pump, a gas blowing pipe and a first gas guide pipe, the base of the first mechanical arm is fixedly installed on the mobile mechanism, the gas collection cage is installed on the extending end of the first mechanical arm, the gas collection cage is composed of a folding framework, the outside of the folding framework is covered with a film, the gas blowing pipe is arranged along the extending direction of the folding framework, the leading end of the gas blowing pipe is connected with the gas blowing pump, the trailing end of the gas blowing pipe is provided with a gas outlet for blowing gas to the plant branch and leaf, the gas inlet end of the first gas guide pipe is connected with the gas collection cage, and the gas outlet end of the first gas guide pipe is connected with the animal detection box. The animal detection box is fixed on the mobile mechanism, the animal detection box has a moving chamber for accommodating animals, and the first gas guide pipe is connected with the moving chamber. The mobile mechanism is a walking chassis, and the mobile mechanism further carries a root gas collection device, the root gas collection device includes a second mechanical arm, a gas collection pile for extending into the soil of the plant roots, a probe device, a gas suction pump and a second gas guide pipe, the base of the second mechanical arm is fixedly installed on the walking chassis, the gas collection pile is installed on the extending end of the second mechanical arm, the probe device is embedded in the gas collection pile, the probe device is connected with the animal detection box through the second gas guide pipe, the gas suction pump provides power to send gas from the probe device to the animal detection box, and the end of the probe device is provided with a filter screen for forming a barrier to the soil. The gas outlet of the moving chamber is connected with a gas harmless treatment device.

2. The plant disease gas collection detection device of claim 1, wherein: The gas collection cage has an unfolded state and a folded state. In the unfolded state, the folding framework is unfolded, the film is opened, a cage structure is formed, and the plant stem and leaf can be covered. In the folded state, the folding framework is contracted and accommodated close to the first mechanical arm.

3. The plant disease gas collection detection device of claim 2, wherein: The folding framework includes a top support, a side support and a bottom support connected in sequence, a connecting frame and a ball screw, the ball screw is connected with the output end of the first mechanical arm, the top support, the side support and the bottom support form a framework unit, the framework unit is circularly arranged along the axis of the ball screw, the top support is connected with the sliding nut of the ball screw through the connecting frame, and the unfolded state and the folded state of the gas collection cage are switched by a power source.

4. The plant disease gas collection detection device of claim 1, wherein: The walking chassis is a tracked vehicle.

5. The plant disease gas collection detection device of claim 1, wherein: The walking chassis is provided with an operation table for controlling the first mechanical arm and the second mechanical arm.

6. The plant disease gas collection detection device of claim 1, wherein: The animal detection box contains rats for detecting gas.

7. The method of operating a plant disease gas collection detection apparatus according to any one of claims 1 to 6, wherein, The mobile mechanism moves to the plant to be detected, the stem and leaf gas collection device covers the branches and leaves of the plant to be detected and collects gas, the gas collected by the stem and leaf gas collection device is transported to the animal detection box, and whether the plant is infected with a disease is determined according to the reaction of a pre-trained animal. ​

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