A method, feed and device for raising **Pristhesancus plagipennis** using fruit flies with vestigial wings

Abstract

The present invention relates to the technical field of natural enemy insect breeding, and particularly to a method, feed and device for breeding **Pristhesancus plagipennis** using wingless fruit flies. The present invention provides a method for breeding **Pristhesancus plagipennis** using wingless fruit flies, including: breeding **Pristhesancus plagipennis** with wingless fruit fly adults. Through experimental research, the present invention discovers that the predation preference of **Pristhesancus plagipennis** is that it prefers to prey on wingless fruit fly adults. Based on the predation characteristics of **Pristhesancus plagipennis** in different insect states, the present invention uses wingless fruit fly adults to breed **Pristhesancus plagipennis**, eliminating the cumbersome steps of planting host plants to breed prey, reducing the breeding cost, and improving the efficiency of breeding **Pristhesancus plagipennis**. In addition, by using the device provided by the present invention, it is possible to complete the entire generation of breeding **Pristhesancus plagipennis** with wingless fruit flies indoors.

Description

Technical Field

[0001] The present invention relates to the technical field of natural enemy insect breeding, and particularly to a method, feed, and device for breeding Rhynocoris fuscipes using wingless fruit flies. Background Art

[0002] Rhynocoris fuscipes (Fabricius) is a natural enemy insect in the family Reduviidae with strong predation ability. It can prey on various pests such as Helicoverpa armigera on cotton, Nilaparvata lugens, Chilo suppressalis, and Cnaphalocrocis medinalis on rice, Myzus persicae, Helicoverpa assulta, Spodoptera litura on tobacco, the pest Myllocerus undecimpustulatus on mulberry, and Eutectona machaeralis on teak. It has a very broad application prospect in the prevention and control of crop pests. There are few reports on the artificial breeding technology of this natural enemy. Patent No. CN 112674034A discloses a method for breeding Rhynocoris fuscipes, which first plants Sonchus asper in flower pots, then breeds aphids with Sonchus asper, and then feeds Rhynocoris fuscipes with aphids. Although this method can complete the generation breeding of Rhynocoris fuscipes, it requires continuous planting of host plants, and the breeding of host plants consumes a lot of time and labor. Therefore, it is necessary to further seek a simpler operation and lower breeding cost artificial breeding mode. Summary of the Invention

[0003] To solve the above problems, the present invention provides a method, feed, and device for breeding Rhynocoris fuscipes using wingless fruit flies. Using the method of the present invention to breed Rhynocoris fuscipes eliminates the cumbersome steps of planting host plants to breed prey, reduces the breeding cost, and improves the efficiency of breeding Rhynocoris fuscipes.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] The present invention provides a method for breeding Rhynocoris fuscipes using wingless fruit flies, comprising: feeding adult wingless fruit flies to Rhynocoris fuscipes.

[0006] Preferably, the adult wingless fruit flies include adult Drosophila melanogaster and adult Drosophila hydei. Adult Drosophila melanogaster are used to feed the 1st - 3rd instar nymphs of Rhynocoris fuscipes, and adult Drosophila hydei are used to feed the nymphs of Rhynocoris fuscipes older than the 3rd instar and / or adult Rhynocoris fuscipes.

[0007] Preferably, the method for obtaining the adult wingless fruit flies includes:

[0008] Feeding adult wingless fruit flies with feed, the adult wingless fruit flies mate and lay eggs, and after the eggs hatch, wingless fruit fly larvae are obtained;

[0009] The larvae of the vestigial-winged Drosophila melanogaster pupate after feeding on the feed, and the pupae emerge into adult vestigial-winged Drosophila melanogaster.

[0010] Preferably, the temperature for hatching is 25±1°C and the humidity is 70%±10%.

[0011] The present invention also provides a feed for breeding adult vestigial-winged Drosophila melanogaster in the method described in the above technical solution, including feed A and / or feed B in independent packages;

[0012] The feed A includes the following raw materials for preparation in parts by mass: 500 parts of water, 100-120 parts of corn flour, 100-120 parts of potato granules, 10-12 parts of cinnamon, 20-30 parts of alfalfa grass powder, 20 parts of dried kelp powder, 20 parts of dried watermelon powder, 20 parts of yeast powder, 8-10 parts of rose hips, 8-10 parts of hibiscus flowers, 8-10 parts of marigold flowers, 3-5 parts of red pepper powder, 3-5 parts of turmeric root powder, 8-10 parts of fructose, 2 parts of compound vitamins, 1 part of methylparaben, 0.5-0.8 parts of table salt, 0.5-0.8 parts of citrate, 0.5-0.8 parts of calcium malate, 0.5-0.8 parts of zinc methionine, 0.5-0.8 parts of magnesium glycinate chelate, 0.5-0.8 parts of copper methionine, and 0.5-0.8 parts of calcium propionate;

[0013] The feed B includes the following raw materials for preparation in parts by mass: 500 parts of water, 100-120 parts of corn flour, 100-120 parts of flour, 20-30 parts of fruit puree, 10-15 parts of cinnamon, 10-15 parts of alfalfa grass powder, 10-15 parts of rose flowers, 10-15 parts of hibiscus flowers, 10-15 parts of yeast powder, 8-10 parts of fructose, 2-3 parts of malic acid, 2 parts of potassium citrate, 1 part of vitamin A supplement, 1 part of vitamin D supplement, 0.5-0.8 parts of zinc methionine, 0.5-0.8 parts of copper methionine, 1 part of methylparaben, 1 part of potassium sorbate, 0.8-1.0 parts of choline chloride, 0.8-1.0 parts of pantothenic acid, 0.5-0.8 parts of β-carotene, 0.5 parts of pyridoxine hydrochloride, 0.5-1.0 parts of folic acid, and vitamin B 12 0.5 parts.

[0014] The present invention also provides a device for raising Rhynocoris marginatus using the method described in the above technical solution, including:

[0015] At least one feed box (10) having a feed cavity (11) for containing feed;

[0016] A vestigial-winged Drosophila melanogaster breeding box (20) having a breeding cavity (21), wherein the feed box (10) and the vestigial-winged Drosophila melanogaster breeding box (20) can be detachably connected; a plurality of adult vestigial-winged Drosophila melanogaster are placed in the breeding cavity (21), and the plurality of adult vestigial-winged Drosophila melanogaster can lay eggs in the feed cavity (11);

[0017] The vestigial-wing Drosophila culture box (30) has a culture cavity (31). The feed box (10) in the breeding cavity (21) and the vestigial-wing Drosophila culture box (30) can be detachably connected. An escape hole (32) that can communicate the culture cavity (31) with the outside is provided on the vestigial-wing Drosophila culture box (30), and a blocking component that can block the culture cavity (31) and the outside is provided at the escape hole (32). The blocking component and the vestigial-wing Drosophila culture box (30) can be detachably connected.

[0018] The red-colored Rhinocoris fuscipes rearing box (40) has a rearing cavity (41) in which red-colored Rhinocoris fuscipes are reared. An access hole (42) that can communicate the rearing cavity (41) with the outside is provided on the red-colored Rhinocoris fuscipes rearing box (40); and a connecting pipe (50) whose two ends are respectively used to be detachably connected and communicate with the escape hole (32) and the access hole (42). The adults cultured in the vestigial-wing Drosophila culture box (30) can enter the red-colored Rhinocoris fuscipes rearing box (40) through the escape hole (32), the connecting pipe (50), and the access hole (42).

[0019] Preferably, ventilation holes (60) are provided on the vestigial-wing Drosophila breeding box (20), the vestigial-wing Drosophila culture box (30), and the red-colored Rhinocoris fuscipes rearing box (40); and the escape hole (32) is provided at the top of the vestigial-wing Drosophila culture box (30).

[0020] Preferably, the number of the vestigial-wing Drosophila breeding boxes (20) and the vestigial-wing Drosophila culture boxes (30) is at least two; the two vestigial-wing Drosophila breeding boxes (20) are respectively a first breeding box and a second breeding box, and the two vestigial-wing Drosophila culture boxes (30) are respectively a first culture box and a second culture box.

[0021] The first breeding box and the second breeding box are respectively used to place adult vestigial-wing small Drosophila and adult vestigial-wing large Drosophila. The feed box (10) in the first breeding box is used to contain the feed for vestigial-wing small Drosophila, and the feed box (10) in the second breeding box is used to contain the feed for vestigial-wing large Drosophila.

[0022] The first culture box is used to place the feed box (10) in the first breeding box and cultivate adult vestigial-wing small Drosophila.

[0023] The second culture box places the feed box (10) in the second breeding box and cultivates adult vestigial-wing large Drosophila.

[0024] The red-colored Rhinocoris fuscipes rearing box (40) is communicated with the first culture box or the second culture box through the connecting pipe (50).

[0025] Preferably, a fixing component (90) is fixedly arranged in both the wingless fruit fly breeding box (20) and the wingless fruit fly culture box (30). The upper end of the feed box (10) is open, and the fixing component (90) is used for detachably connecting with the feed box (10).

[0026] Preferably, each fixing component (90) includes a connecting rod (91) and a connecting disk (92). The feed box (10) is arranged in a cylindrical shape. One end of the connecting rod (91) is fixedly connected to the top inside the wingless fruit fly breeding box (20) or the wingless fruit fly culture box (30), and the other end is fixedly connected to the connecting disk (92). The connecting disk (92) is detachably connected to the upper end of the feed box (10), and a worm passing hole (93) is arranged on the connecting disk (92) to communicate the feed cavity (11) with the breeding cavity (21) or the culture cavity (31) and allow wingless fruit flies to pass through.

[0027] Beneficial effects:

[0028] The present invention provides a method for raising Rhynocoris fuscipes using wingless fruit flies, including: raising Rhynocoris fuscipes with adult wingless fruit flies. Through experimental research, the present invention discovers that the predation preference characteristic of Rhynocoris fuscipes is that it prefers to prey on adult wingless fruit flies. Based on the predation characteristics of Rhynocoris fuscipes in different insect states, the present invention raises Rhynocoris fuscipes with adult wingless fruit flies, eliminating the cumbersome steps of planting host plants to breed prey, reducing the breeding cost, and improving the efficiency of raising Rhynocoris fuscipes. Description of the drawings

[0029] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the following described drawings are only some embodiments of the device of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0030] Figure 1 An isometric schematic diagram of the device for raising Rhynocoris fuscipes using wingless fruit flies provided for the embodiment;

[0031] Figure 2 A front view schematic diagram of the connection structure between the wingless fruit fly culture box and the Rhynocoris fuscipes breeding box provided for the embodiment;

[0032] Figure 3 For Figure 2 A top view schematic diagram of the connection structure between the wingless fruit fly culture box and the Rhynocoris fuscipes breeding box provided;

[0033] Figure 4 For Figure 2Left view schematic diagram of the structure connecting the culture box for wingless fruit flies and the rearing box for Rhinocoris fuscipes

[0034] Figure 5 Schematic diagram of the structure of the second climbing component for wingless fruit flies and Rhinocoris fuscipes provided for the embodiment

[0035] Icons: 1 - Device for rearing Rhinocoris fuscipes using wingless fruit flies; 10 - Feed box; 11 - Feed cavity; 20 - Reproduction box for wingless fruit flies; 21 - Reproduction cavity; 30 - Culture box for wingless fruit flies; 31 - Culture cavity; 32 - Escape hole; 40 - Rearing box for Rhinocoris fuscipes; 41 - Rearing cavity; 42 - Entrance hole; 50 - Connecting pipe; 60 - Ventilation hole; 70 - Cover body; 80 - Box body; 90 - Fixing component; 91 - Connecting rod; 92 - Connecting disc; 93 - Insect passage hole; 100 - Insect-proof component; 110 - Second climbing component; 111 - Support frame; 112 - Climbing board Detailed implementation mode

[0036] The present invention provides a method for rearing Rhinocoris fuscipes using wingless fruit flies, including: rearing Rhinocoris fuscipes with adult wingless fruit flies

[0037] Preferably, the present invention uses adult Drosophila melanogaster to rear the 1st - 3rd instar nymphs of Rhinocoris fuscipes, and preferably uses adult Drosophila hydei to rear the nymphs of Rhinocoris fuscipes older than the 3rd instar and / or adult Rhinocoris fuscipes

[0038] According to the predation characteristics of nymphs or adults at different stages, the present invention correspondingly rears adult Drosophila melanogaster or adult Drosophila hydei, which can complete the whole generation of rearing Rhinocoris fuscipes with wingless fruit flies, omitting the cumbersome steps of planting host plants to breed prey, reducing the rearing cost, and improving the efficiency of rearing Rhinocoris fuscipes

[0039] In the present invention, the preferred method for obtaining adult wingless fruit flies preferably includes:

[0040] Feeding adult wingless fruit flies with feed, the adult wingless fruit flies mate and lay eggs, and the eggs hatch into wingless fruit fly larvae

[0041] The wingless fruit fly larvae are reared into pupae, and the pupae emerge into adult wingless fruit flies

[0042] Preferably, the present invention feeds adult wingless fruit flies with feed, and the adult wingless fruit flies mate and lay eggs to obtain wingless fruit fly larvae. In the present invention, the adult wingless fruit flies that mate and lay eggs are preferably used as parents and continuously lay eggs

[0043] After obtaining the larvae of Drosophila melanogaster with vestigial wings, the present invention preferably pupates the larvae of Drosophila melanogaster with vestigial wings and hatches them to obtain adults of Drosophila melanogaster with vestigial wings. In the present invention, the temperature for hatching is preferably 25±1°C, and the humidity is preferably 70%±10%. During the process of hatching the larvae of Drosophila melanogaster with vestigial wings to adults of Drosophila melanogaster with vestigial wings, the feed fed is preferably the same as the feed for adults of Drosophila melanogaster with normal wings in the above technical solution.

[0044] The present invention also provides a feed for breeding the adults of Drosophila melanogaster with vestigial wings in the above technical solution, including independently packaged Feed A and / or Feed B;

[0045] Feed A includes the following preparation raw materials in parts by mass: 500 parts of water, 100-120 parts of corn flour, 100-120 parts of potato granules, 10-12 parts of cinnamon, 20-30 parts of alfalfa meal, 20 parts of dried kelp powder, 20 parts of dried watermelon powder, 20 parts of yeast powder, 8-10 parts of rose hips, 8-10 parts of hibiscus flowers, 8-10 parts of marigold flowers, 3-5 parts of red pepper powder, 3-5 parts of turmeric root powder, 8-10 parts of fructose, 2 parts of compound vitamins, 1 part of methylparaben, 0.5-0.8 parts of table salt, 0.5-0.8 parts of citrate, 0.5-0.8 parts of calcium malate, 0.5-0.8 parts of zinc methionine, 0.5-0.8 parts of magnesium glycinate chelate, 0.5-0.8 parts of copper methionine, and 0.5-0.8 parts of calcium propionate;

[0046] Feed B includes the following preparation raw materials in parts by mass: 500 parts of water, 100-120 parts of corn flour, 100-120 parts of flour, 20-30 parts of fruit puree, 10-15 parts of cinnamon, 10-15 parts of alfalfa meal, 10-15 parts of rose flowers, 10-15 parts of hibiscus flowers, 10-15 parts of yeast powder, 8-10 parts of fructose, 2-3 parts of malic acid, 2 parts of potassium citrate, 1 part of vitamin A supplement, 1 part of vitamin D supplement, 0.5-0.8 parts of zinc methionine, 0.5-0.8 parts of copper methionine, 1 part of methylparaben, 1 part of potassium sorbate, 0.8-1.0 parts of choline chloride, 0.8-1.0 parts of pantothenic acid, 0.5-0.8 parts of β-carotene, 0.5 parts of pyridoxine hydrochloride, 0.5-1.0 parts of folic acid, and 0.5 part of vitamin B 12 0.5 part.

[0047] Unless otherwise specified, there are no special requirements for the sources of the components in Feed A or Feed B of the present invention, and commercially available products well-known to those skilled in the art can be used.

[0048] Feed A of the present invention includes 500 parts by mass of water.

[0049] Based on the parts by mass of the water, Feed A of the present invention comprises 100 - 120 parts of corn flour, preferably 110 - 120 parts, and more preferably 120 parts. The corn flour of the present invention can improve the fecundity of fruit flies, shorten the pre-oviposition period of fruit flies, prolong the larval stage of fruit flies, and shorten the pupal stage of fruit flies.

[0050] Based on the parts by mass of the water, Feed A of the present invention comprises 100 - 120 parts of potato granules, preferably 110 - 120 parts, and more preferably 120 parts. The potato powder of the present invention can promote the gastrointestinal digestion of fruit flies.

[0051] Based on the parts by mass of the water, Feed A of the present invention comprises 10 - 12 parts of cinnamon, preferably 10 - 11 parts, and more preferably 10 parts. The cinnamon of the present invention can promote the metabolic process of digestive enzymes in fruit flies, and can also inhibit the growth of bacteria to prevent Feed A from rotting.

[0052] Based on the parts by mass of the water, Feed A of the present invention comprises 20 - 30 parts of alfalfa powder, preferably 25 - 30 parts, and more preferably 30 parts. The alfalfa powder of the present invention can promote the growth and development of fruit flies.

[0053] Based on the parts by mass of the water, Feed A of the present invention comprises 20 parts of dried kelp powder. The dried kelp powder of the present invention can promote the growth of fruit flies and increase the egg-laying amount of fruit flies.

[0054] Based on the parts by mass of the water, Feed A of the present invention comprises 20 parts of dried watermelon powder. The dried watermelon powder of the present invention contains rich substances such as protein, glucose, fructose, malic acid, glutamic acid, citrulline, and sucrase, etc., and can promote the growth and development of fruit flies.

[0055] Based on the parts by mass of the water, Feed A of the present invention comprises 20 parts of yeast powder. In the present invention, the yeast powder preferably comprises brewer's yeast. The yeast powder of the present invention is the nitrogen source component source of Feed A, can produce a small amount of volatile substances with a stimulating odor similar to that of ripe fruits to attract fruit flies to gather and feed, and can also condition the intestine and eliminate indigestion in the gastrointestinal tract of fruit flies.

[0056] Based on the parts by mass of the water, Feed A of the present invention comprises 8 - 10 parts of rose hips, preferably 9 - 10 parts, and more preferably 10 parts. The rose hips of the present invention can improve the immunity of fruit flies.

[0057] Based on the parts by mass of the water, Feed A of the present invention comprises 8 - 10 parts of hibiscus flowers, preferably 9 - 10 parts, and more preferably 10 parts. The hibiscus flowers of the present invention have the functions of sterilization and enhancing the physique of fruit flies.

[0058] Based on the mass parts of the water, Feed A of the present invention comprises 8 - 10 parts of marigold flowers, preferably 9 - 10 parts, and more preferably 10 parts. The marigold flowers of the present invention contain various amino acids and flavonoid substances and have the effect of antioxidation.

[0059] Based on the mass parts of the water, Feed A of the present invention comprises 3 - 5 parts of red pepper powder, preferably 4 - 5 parts, and more preferably 5 parts. The red pepper powder of the present invention can promote the salivary secretion of fruit flies, promote digestion and improve appetite.

[0060] Based on the mass parts of the water, Feed A of the present invention comprises 3 - 5 parts of turmeric root powder, preferably 4 - 5 parts, and more preferably 5 parts. The turmeric root powder of the present invention has antioxidant and anti-inflammatory activities and can delay the aging of fruit flies and prolong the lifespan of fruit flies.

[0061] Based on the mass parts of the water, Feed A of the present invention comprises 8 - 10 parts of fructose, preferably 9 - 10 parts, and more preferably 10 parts. The fructose of the present invention can regulate the insulin activity of fruit flies.

[0062] Based on the mass parts of the water, Feed A of the present invention comprises 2 parts of compound vitamins. The compound vitamins of the present invention are preferably purchased from Wyeth Pharmaceuticals Co., Ltd. with the product number 6675752. The compound vitamins of the present invention can supplement the vitamins required by fruit flies and accelerate the growth and development speed.

[0063] Based on the mass parts of the water, Feed A of the present invention comprises 1 part of methyl paraben.

[0064] Based on the mass parts of the water, Feed A of the present invention comprises 0.5 - 0.8 parts of table salt, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts. By compounding an appropriate amount of table salt, the present invention can accelerate the growth and development of fruit fly larvae and increase the number of offspring.

[0065] Based on the mass parts of the water, Feed A of the present invention comprises 0.5 - 0.8 parts of citrate, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts. In the present invention, the citrate preferably comprises sodium citrate and / or potassium citrate, and more preferably sodium citrate. The citrate of the present invention can improve the activity and reproductive ability of fruit flies and prolong the lifespan of fruit flies.

[0066] Based on the mass parts of the water, Feed A of the present invention comprises 0.5 - 0.8 parts of calcium malate, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts.

[0067] Based on the mass parts of the water, Feed A of the present invention comprises 0.5 - 0.8 parts of zinc methionine, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts.

[0068] Based on the mass parts of the water, Feed A of the present invention comprises 0.5 - 0.8 parts of magnesium glycinate chelate, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts.

[0069] Based on the mass parts of the water, Feed A of the present invention comprises 0.5 - 0.8 parts of copper methionine, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts.

[0070] The methionine zinc, magnesium glycinate chelate and copper methionine of the present invention are all nutritional fortifiers, which can enhance the immunity of fruit flies and improve the survival rate of fruit flies.

[0071] Based on the mass parts of the water, Feed A of the present invention comprises 0.5 - 0.8 parts of calcium propionate, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts. The calcium propionate of the present invention can promote the reproductive development of fruit flies and enhance immunity.

[0072] Feed A obtained by compounding appropriate amounts of each component of the present invention can be used to feed adult and larval Drosophila melanogaster with vestigial wings, and has the effects of promoting the growth and development of larvae, shortening the development cycle, increasing the weight and number of adult Drosophila melanogaster, and prolonging the lifespan of adults.

[0073] Feed B of the present invention comprises 500 mass parts of water.

[0074] Based on the mass parts of the water in Feed B, Feed B of the present invention comprises 100 - 120 parts of corn flour, preferably 110 - 120 parts, and more preferably 120 parts. The function of the corn flour of the present invention is the same as above and will not be elaborated here.

[0075] Based on the mass parts of the water in Feed B, Feed B of the present invention comprises 100 - 120 parts of flour, preferably 110 - 120 parts, and more preferably 120 parts.

[0076] Based on the mass parts of the water in Feed B, Feed B of the present invention comprises 20 - 30 parts of fruit puree, preferably 20 - 25 parts, and more preferably 20 parts. In the present invention, the fruit puree preferably comprises banana puree or apple puree, and more preferably apple puree. The fruit puree of the present invention is rich in dietary fiber and organic acids, and can well stimulate the taste bud sensitivity and promote the secretion of digestive juice in the gastrointestinal tract of fruit flies.

[0077] Based on the mass parts of the water in Feed B, Feed B of the present invention comprises 10 - 15 parts of cinnamon, preferably 10 - 13 parts, and more preferably 10 parts. The function of the cinnamon of the present invention is the same as above and will not be elaborated here.

[0078] Based on the mass portion of water in Feed B, Feed B of the present invention includes 10 - 15 parts of alfalfa meal, preferably 12 - 15 parts, and more preferably 15 parts. The function of the alfalfa meal in the present invention is the same as above and will not be elaborated here.

[0079] Based on the mass portion of water in Feed B, Feed B of the present invention includes 10 - 15 parts of rose flowers, preferably 12 - 15 parts, and more preferably 15 parts.

[0080] Based on the mass portion of water in Feed B, Feed B of the present invention includes 10 - 15 parts of hibiscus flowers, preferably 12 - 15 parts, and more preferably 15 parts.

[0081] The rose flowers and hibiscus flowers in the present invention contain various vitamins, aromatic substances, and trace elements, which can promote the growth and development of fruit flies.

[0082] Based on the mass portion of water in Feed B, Feed B of the present invention includes 10 - 15 parts of yeast powder, preferably 10 - 13 parts, and more preferably 10 parts. The function of the yeast powder in the present invention is the same as above and will not be elaborated here.

[0083] Based on the mass portion of water in Feed B, Feed B of the present invention includes 8 - 10 parts of fructose, preferably 9 - 10 parts, and more preferably 10 parts. The function of the fructose in the present invention is the same as above and will not be elaborated here.

[0084] Based on the mass portion of water in Feed B, Feed B of the present invention includes 2 - 3 parts of malic acid, preferably 2.5 - 3 parts, and more preferably 3 parts. The malic acid in the present invention can increase the secretion of fruit fly saliva and increase the appetite of fruit flies.

[0085] Based on the mass portion of water in Feed B, Feed B of the present invention includes 2 parts of potassium citrate. The potassium citrate in the present invention can adjust the taste of Feed B and increase the appetite of fruit flies.

[0086] Based on the mass portion of water in Feed B, Feed B of the present invention includes 1 part of vitamin A supplement.

[0087] Based on the mass portion of water in Feed B, Feed B of the present invention includes 1 part of vitamin D supplement. The present invention has no special requirements for the sources of the vitamin A supplement or vitamin D supplement, and commercially available products well-known to those skilled in the art can be used.

[0088] Based on the mass portion of water in Feed B, Feed B of the present invention includes 0.5 - 0.8 parts of zinc methionine, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts.

[0089] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 0.5 - 0.8 parts of copper methionine, preferably 0.6 - 0.8 parts, and more preferably 0.8 parts.

[0090] The functions of zinc methionine and copper methionine of the present invention are the same as above and will not be elaborated here.

[0091] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 1 part of methylparaben.

[0092] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 1 part of potassium sorbate. Methylparaben and potassium sorbate of the present invention are preservatives.

[0093] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 0.8 - 1.0 parts of choline chloride, preferably 0.9 - 1.0 parts, and more preferably 1.0 part. Choline chloride of the present invention can stimulate fruit flies' ovaries to lay more eggs.

[0094] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 0.8 - 1.0 parts of pantothenic acid, preferably 0.9 - 1.0 parts, and more preferably 1.0 part. Pantothenic acid of the present invention can maintain the normal functions of the digestive tract of fruit flies.

[0095] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 0.5 - 0.8 parts of β - carotene, preferably 0.5 - 0.7 parts, and more preferably 0.5 part. β - carotene of the present invention has the functions of maintaining cell viability, delaying aging, and can be converted into vitamin A.

[0096] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 0.5 part of pyridoxine hydrochloride.

[0097] Based on the mass portion of water in Feed B, Feed B of the present invention comprises 0.5 - 1.0 parts of folic acid, preferably 0.7 - 1.0 parts, and more preferably 1.0 part.

[0098] Based on the mass portion of water in Feed B, Feed B of the present invention comprises vitamin B 12 0.5 part.

[0099] Feed B obtained by compounding appropriate amounts of each component in the present invention can be used to raise adult and larval Drosophila melanogaster with vestigial wings, and has the effects of promoting the growth and development of larvae, shortening the development cycle, increasing the weight and number of adult Drosophila melanogaster, and prolonging the lifespan of adults.

[0100] The present invention preferably also provides a preparation method of the above - mentioned Feed A or B, comprising the following steps:

[0101] Except for water, 80 wt.% of yeast powder and other preparation raw materials are mixed to obtain a mixed raw material;

[0102] After boiling water, it is mixed with the mixed raw material and stirred to obtain a stirred raw material;

[0103] When the temperature of the stirred raw material drops to 55 - 65 °C, the remaining 20% of yeast powder is sprinkled on the stirred raw material to obtain the feed A or B.

[0104] In the present invention, cinnamon, rose hips, hibiscus flowers or marigold flowers are preferably pulverized and sieved through a 60 - mesh sieve before being mixed with yeast powder. The present invention has no special requirements for the pulverization method, and a method well - known to those skilled in the art can be used.

[0105] In the present invention, by adding yeast powder in two steps, the first part of yeast powder can be mixed with the culture medium to facilitate the fermentation and softening of the culture medium, and the second part sprinkled on the surface of the culture medium is conducive to the formation of yeast on the surface of the culture medium for fruit flies to eat.

[0106] An apparatus 1 for raising Rhynocoris fuscipes with wing - less fruit flies provided by an embodiment of the present invention is shown in Figures 1 to 4 , including a wing - less fruit fly breeding box 20, a wing - less fruit fly culture box 30, a Rhynocoris fuscipes breeding box 40, a connecting pipe 50 and at least one feed box 10; the feed box 10 has a feed cavity 11 for containing feed; the wing - less fruit fly breeding box 20 has a breeding cavity 21, and the feed box 10 and the wing - less fruit fly breeding box 20 can be detachably connected; a plurality of wing - less fruit fly adults are placed in the breeding cavity 21, and the plurality of wing - less fruit fly adults can lay eggs in the feed cavity 11; the wing - less fruit fly culture box 30 has a culture cavity 31, and the feed box 10 in the breeding cavity 21 and the wing - less fruit fly culture box 30 can be detachably connected; an escape hole 32 communicating the culture cavity 31 and the outside is provided on the wing - less fruit fly culture box 30, and a blocking member for closing the culture cavity 31 and the outside is provided at the escape hole 32, and the blocking member and the wing - less fruit fly culture box 30 can be detachably connected; the Rhynocoris fuscipes breeding box 40 has a breeding cavity 41, and Rhynocoris fuscipes are raised in the breeding cavity 41, and an access hole 42 communicating the breeding cavity 41 and the outside is provided on the Rhynocoris fuscipes breeding box 40; both ends of the connecting pipe 50 are respectively used to be detachably connected and communicate with the escape hole 32 and the access hole 42, and the adults cultured in the wing - less fruit fly culture box 30 can enter the Rhynocoris fuscipes breeding box 40 through the escape hole 32, the connecting pipe 50 and the access hole 42.

[0107] Adult Drosophila melanogaster with vestigial wings in the breeding cavity 21 can lay eggs in the feed box 10 within the breeding cavity 21. Then, the feed box 10 containing Drosophila melanogaster eggs with vestigial wings in the breeding cavity 21 is removed from the Drosophila melanogaster breeding box 20 and placed into the culture cavity 31. A new feed box 10 is set in the breeding cavity 21 for adult Drosophila melanogaster with vestigial wings to continue laying eggs continuously to meet the feeding requirements. After the Drosophila melanogaster eggs with vestigial wings in the feed box 10 in the culture cavity 31 are cultivated into adult Drosophila melanogaster with vestigial wings, the blocking component is removed to open the escape hole 32, and the escape hole 32 and the entry hole 42 are connected through the connecting pipe 50. So that adult Drosophila melanogaster with vestigial wings enter the Rhynocoris fuscipes rearing box 40 through the escape hole 32, the connecting pipe 50, and the entry hole 42, and are preyed on by Rhynocoris fuscipes in the rearing cavity 41. In this way, only adult Drosophila melanogaster with vestigial wings need to be bred with feed indoors, and then adult Drosophila melanogaster with vestigial wings are used to rear Rhynocoris fuscipes, eliminating the cumbersome steps of planting host plants to breed prey, reducing the feeding cost, and improving the efficiency of rearing Rhynocoris fuscipes.

[0108] Specifically, the blocking component can be set as absorbent cotton.

[0109] In an alternative embodiment of the present example, preferably, ventilation holes 60 are provided on the Drosophila melanogaster breeding box 20, the Drosophila melanogaster culture box 30, and the Rhynocoris fuscipes rearing box 40. The provision of the ventilation holes 60 facilitates the normal respiration of Drosophila melanogaster with vestigial wings. The escape hole 32 is provided at the top of the Drosophila melanogaster culture box 30. Utilizing the upward climbing tendency of adult Drosophila melanogaster with vestigial wings, adult Drosophila melanogaster with vestigial wings can enter the connecting pipe 80 through the escape hole 32 and continuously enter the Rhynocoris fuscipes rearing box 40 along the connecting pipe 80.

[0110] In an alternative embodiment of the present example, preferably, the Drosophila melanogaster breeding box 20, the Drosophila melanogaster culture box 30, and the Rhynocoris fuscipes rearing box 40 all include a lid body 70 and a box body 80 that can be detachably connected, facilitating the assembly and replacement of the feed box 10. The escape hole 32 is provided on the lid body 70 of the Drosophila melanogaster culture box 30, and the entry hole 42 is provided on the lid body 70 of the Rhynocoris fuscipes rearing box 40. Utilizing the upward climbing tendency of adult Drosophila melanogaster with vestigial wings, adult Drosophila melanogaster with vestigial wings can enter the connecting pipe 80 through the escape hole 32 and continuously enter the Rhynocoris fuscipes rearing box 40 along the connecting pipe 80.

[0111] Furthermore, the entry hole 42 can also be provided on the bottom wall of the Rhynocoris fuscipes rearing box 40, and the Rhynocoris fuscipes rearing box 40 is located above the Drosophila melanogaster culture box 30.

[0112] Among the optional schemes of the present embodiment, it is more preferred that, according to the predation ability of the red-colored assassin bug at different ages, the nymphs of the red-colored assassin bug of 1 to 3 ages are fed with the adults of the small malnourished fruit flies, and the nymphs and / or adults of the red-colored assassin bug of more than 3 ages are fed with the adults of the large malnourished fruit flies, so as to realize the artificial rearing of the entire generation of the red-colored assassin bug; therefore, the malnourished fruit fly breeding box 20 and the malnourished fruit fly culture box 30 are both set to at least two, preferably, both can be set to two, the two malnourished fruit fly breeding boxes 20 are respectively the first breeding box and the second breeding box, and the two malnourished fruit fly culture boxes 30 are respectively the first culture box and the second culture box. Two culture boxes; the first breeding box and the second breeding box are used to place the adults of the small fruit flies with stump wings and the adults of the large fruit flies with stump wings respectively, the feed box 10 in the first breeding box is used to contain the feed of the small fruit flies with stump wings, and the feed box 10 in the second breeding box is used to contain the feed of the large fruit flies with stump wings; the first culture box is used to place the feed box 10 in the first breeding box and culture the adults of the small fruit flies with stump wings; the second culture box is used to place the feed box 10 in the second breeding box and culture the adults of the large fruit flies with stump wings; the red-colored assassin bug breeding box 40 is connected to the first culture box or the second culture box through a connecting pipe 50.

[0113] Specifically, when the red-colored assassin bugs raised in the red-colored assassin bug breeding box 40 are in the 1st to 3rd instar nymphs, they are fed with adult pterygoid fruit flies. For the cultivation of pterygoid fruit flies, after the adult pterygoid fruit flies mate and lay eggs in the first breeding box for 4 to 5 days, before the fruit fly larvae crawl out of the feed box 10, the feed box 10 in the first breeding box is removed, and a feed box 10 containing new pterygoid fruit fly feed is reinstalled in the first breeding box for the adult pterygoid fruit flies to continue laying eggs. The removed feed box 10 containing the laid eggs is placed in the first culture box, and the pterygoid fruit fly eggs are hatched, and the larvae crawl out of the inner wall of the first culture box and pupate for standby use. When the pupae in the first culture box begin to emerge as adults and the number of adults reaches more than 30, the blocking component on the escape hole 32 of the first culture box is removed, and the escape hole 32 is connected to the entrance hole 42 of the red-colored assassin bug breeding box 40 through the connecting pipe 50;

[0114] When the red-colored assassin bugs raised in the red-colored assassin bug breeding box 40 are larger than the third-instar nymphs, they are fed with adult drosophila with vestigial wings. For the cultivation of drosophila with vestigial wings, after the adult drosophila with vestigial wings mates and lays eggs in the second breeding box for 7 to 8 days, before the fruit fly larvae crawl out of the feed box 10, the feed box 10 in the second breeding box is removed, and a feed box 10 containing new drosophila with vestigial wings feed is reinstalled in the second breeding box for the drosophila with vestigial wings adults to continue laying eggs. The feed box 10 is placed in the second culture box, and when the winged Drosophila eggs are hatched, the larvae crawl out of the inner wall of the second culture box and pupate for standby use, when the pupae in the second culture box begin to emerge into adults, and when the number of adults reaches more than 30, the blocking component on the escape hole 32 of the second culture box is removed, and the escape hole 32 is connected to the entrance hole 42 of the red-colored assassin bug breeding box 40 through the connecting pipe 50; thus, the artificial breeding of the entire generation of red-colored assassin bugs in the red-colored assassin bug breeding box 40 is realized. In the present invention, the incubation temperature is preferably 25±1°C, and the humidity is preferably 70%±10%.

[0115] Further preferably, the number of the connecting tubes 50 can be set to two, and the two connecting tubes 50 are respectively used to connect the first culture box and the second culture box with the red-colored assassin bug breeding box 40.

[0116] Further preferably, two red-colored assassin bug breeding boxes 40 can also be set. After the red-colored assassin bugs in one red-colored assassin bug breeding box 40 grow to be larger than the third-instar nymphs, at this time, the red-colored assassin bug breeding box 40 does not need to use the first breeding box and the first culture box to cultivate the adult pterygoid fruit flies. Therefore, the cultivation of the adult pterygoid fruit flies in the first breeding box and the first culture box can be used for the cultivation of the 1st to 3rd-instar nymphs of the red-colored assassin bugs in another red-colored assassin bug breeding box 40. In this way, the breeding efficiency of the red-colored assassin bugs is improved.

[0117] In the optional scheme of this embodiment, it is more preferred that a fixing component 90 is fixedly arranged in the winged fruit fly breeding box 20 and the winged fruit fly culture box 30, and the upper end of the feed box 10 is open to facilitate the fruit flies to crawl out of the feed cavity 11. The fixing component 90 is used to be detachably connected to the feed box 10, so that the feed box 10 can be detachably arranged in the winged fruit fly breeding box 20 and the winged fruit fly culture box 30, which is convenient for replacing and transferring the feed box 10.

[0118] Further preferably, each fixing component 90 includes a connecting rod 91 and a connecting plate 92. The feed box 10 is arranged in a cylindrical shape. One end of the connecting rod 91 is fixedly connected to the top inside the wingless fruit fly breeding box 20 or the wingless fruit fly culture box 30, and the other end is fixedly connected to the connecting plate 92. The connecting plate 92 is detachably connected to the upper end of the feed box 10, so as to fix the feed box 10 inside the wingless fruit fly breeding box 20 or the wingless fruit fly culture box 30. And an insect passing hole 93 is provided on the connecting plate 92, which can communicate the feed cavity 11 with the breeding cavity or the culture cavity 31 and is used for the wingless fruit flies to pass through, ensuring that the fruit flies can climb out of the feed cavity 11.

[0119] In an alternative embodiment of the present embodiment, more preferably, an insect-proof component 100 is provided at each ventilation hole 60 to prevent wingless fruit flies or Rhinocoris fuscipes from escaping through the ventilation hole 60. Specifically, the insect-proof component 100 can be set as an insect-proof net.

[0120] In an alternative embodiment of the present embodiment, more preferably, a first climbing component is provided inside both the wingless fruit fly breeding box 20 and the wingless fruit fly culture box 30 to provide an activity place for the wingless fruit flies. Specifically, the first climbing component can be set as wood wool.

[0121] In an alternative embodiment of the present embodiment, more preferably, please refer to Figure 5 , the device 1 for raising Rhinocoris fuscipes with wingless fruit flies provided in this embodiment further includes a second climbing component 110. The second climbing component 110 is arranged inside the Rhinocoris fuscipes breeding box 40, and the second climbing component 110 is used to provide an activity place for Rhinocoris fuscipes and wingless fruit flies inside the Rhinocoris fuscipes breeding box 40.

[0122] Further preferably, the second climbing component 110 includes a support frame 111 and a plurality of climbing plates 112. The plurality of climbing plates 112 are arranged side by side with gaps. Each climbing plate 112 is for Rhinocoris fuscipes and wingless fruit flies to climb on; each climbing plate 112 is detachably connected to the support frame 111, and the size of the activity place is adjusted by adjusting the number of climbing plates 112. Specifically, the middle of the support frame 111 is hollowed out, and each climbing plate 112 is inserted side by side into the hollowed-out area. And a plurality of symmetrically arranged placement grooves are provided on the border of the hollowed-out area of the support frame 111. Each climbing plate 112 can be set as a T shape, and the placement grooves are used for lapping each climbing plate 112. The number of climbing plates 112 is adjusted by lapping to adjust the size of the activity space.

[0123] Specifically, at least the climbing plate 112 can be made of corrugated cardboard; and the number and size of the climbing plates 112 are determined according to actual needs.

[0124] To further illustrate the present invention, a method, feed, and device for breeding Reduvius personatus using wingless Drosophila melanogaster will be described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0125] Example 1

[0126] A feed for breeding adult wingless Drosophila melanogaster consists of the following preparation raw materials:

[0127] Water 500 mL (500 parts), corn flour 120 g (120 parts), potato granules 120 g (120 parts), cinnamon 10 g (10 parts), alfalfa meal 30 g (30 parts), dried kelp powder 20 g (20 parts), dried watermelon powder 20 g (20 parts), yeast powder 20 g (20 parts of brewer's yeast), rose hips 10 g (10 parts), hibiscus flowers 10 g (10 parts), marigold flowers 10 g (10 parts), red pepper powder 5 g (5 parts), turmeric root powder 5 g (5 parts), fructose 10 g (10 parts), compound vitamins 2 g (2 parts), methylparaben 1 g (1 part), table salt 0.8 g (0.8 parts), sodium citrate 0.8 g (0.8 parts), calcium malate 0.8 g (0.8 parts), zinc methionine 0.8 g (0.8 parts), magnesium glycinate chelate 0.8 g (0.8 parts), copper methionine 0.8 g (0.8 parts), and calcium propionate 0.8 g (0.8 parts).

[0128] The feed is prepared by the following preparation method:

[0129] 1) Crush cinnamon, rose hips, hibiscus flowers, and marigold flowers, sieve them through a 60-mesh sieve, and pour them into a stainless steel basin;

[0130] 2) Boil the water and pour it into the stainless steel basin in step 1), stir while pouring, and after stirring evenly, let it stand until the temperature of the stirred material is 60 °C, then mix it with 80 wt.% of yeast powder (16 g) to obtain a primary feed;

[0131] 3) Sprinkle the remaining 20 wt.% of yeast powder (4 g) on the primary feed to obtain the feed for breeding adult wingless Drosophila melanogaster.

[0132] Example 2

[0133] A feed for breeding adult wingless Drosophila magna consists of the following preparation raw materials:

[0134] 500 mL of water (500 parts), 120 g of corn flour (120 parts), 120 g of wheat flour (120 parts), 20 g of apple puree (20 parts), 10 g of cinnamon (10 parts), 15 g of alfalfa powder (15 parts), 15 g of rose flowers (15 parts), 15 g of hibiscus flowers (15 parts), 10 g of yeast powder (10 parts), 10 g of fructose (10 parts), 3 g of malic acid (3 parts), 2 g of potassium citrate (2 parts), 1 g of vitamin A supplement (1 part), 1 g of vitamin D supplement (1 part), 0.8 g of methionine zinc (0.8 part), 0.8 g of methionine copper (0.8 part), 1 g of methylparaben (1 part), 1 g of potassium sorbate (1 part), 1 g of choline chloride (1 part), 1 g of pantothenic acid (1 part), 0.5 g of β-carotene (0.5 part), 0.5 g of pyridoxine hydrochloride (0.5 part), 1 g of folic acid (1 part), and 0.5 g of vitamin B 12 0.5 part).

[0135] The feed is prepared by the following preparation method:

[0136] 1) After crushing cinnamon, rose flowers and hibiscus flowers and passing through a 60-mesh sieve, pour them into a stainless steel basin;

[0137] 2) After boiling the water, pour it into the stainless steel basin in step 1), stir while pouring, and after stirring evenly, let it stand until the temperature of the stirred material is 60 °C, then mix it with 80 wt.% of yeast powder (8 g) to obtain the primary feed;

[0138] 3) Sprinkle the remaining 20 wt.% of yeast powder (2 g) on the primary feed to obtain the feed for breeding adult Drosophila melanogaster with vestigial wings.

[0139] Comparative Examples 1-6

[0140] Feeds similar to those in Example 1, with the dosages of the different preparation raw materials shown in Table 1.

[0141] Table 1 Different feeds for breeding adult Drosophila melanogaster with vestigial wings (unit: g)

[0142]

[0143]

[0144] Note: Except for the components in Table 1 and those in Example 1 being different, the other components in Comparative Examples 1-6 are the same as those in Example 1, and the preparation method is the same as that in Example 1. The difference is that for those with a component dosage of 0, they are not added during preparation.

[0145] Test Example 1

[0146] Determine the effects of the feeds prepared in Example 1 and Comparative Examples 1-6 on the growth and development of Drosophila melanogaster with vestigial wings. The specific treatment methods for each feed are as follows: Anesthetize the adult Drosophila melanogaster with vestigial wings with ether, select 5 pairs of male and female adults, add them to the Drosophila culture bottle containing the feed, and place 10 poplar filaments with a length of 8-12 cm on the feed for the Drosophila to climb. Observe and record the growth and development of different Drosophila every day, and record the development time from Drosophila eggs to pupae, from pupae to adults, the weight of adults, the number of adults, and the lifespan of adults. Each feed is subjected to 3 parallel repeated experiments, and the results are shown in Tables 2-5.

[0147] Table 2 Effects of different feeds on the growth and development of Drosophila melanogaster with vestigial wings (Replicate 1)

[0148]

[0149] Table 3 Effects of different feeds on the growth and development of Drosophila melanogaster with vestigial wings (Replicate 2)

[0150]

[0151]

[0152] Table 4 Effects of different feeds on the growth and development of Drosophila melanogaster with vestigial wings (Replicate 3)

[0153]

[0154] Table 5 Effects of different feeds on the growth and development of Drosophila melanogaster with vestigial wings (Average value)

[0155]

[0156] Note: The data in the table are mean ± standard error. Different letters after the data in the same column indicate differences after t-test comparison. The same applies to the following table.

[0157] As can be seen from Tables 2-5, the feed prepared in Example 1 has the best effect on the growth and development of Drosophila melanogaster with vestigial wings, obtaining the shortest development cycle (8.88 days), increasing the weight of adult Drosophila melanogaster (1.35 mg), getting the largest number of adult Drosophila melanogaster with vestigial wings (57), and the longest average lifespan of adults (15 days).

[0158] Comparative Examples 7-12

[0159] Feeds similar to those in Example 2, with the dosages of the different preparation raw materials shown in Table 6.

[0160] Table 6 Different feeds for breeding adult Drosophila melanogaster with large vestigial wings (unit / g)

[0161]

[0162]

[0163] Note: Except that the components of Comparative Examples 7-12 are different from those of Example 2 in Table 6, the remaining components are the same as those of Example 2, and the preparation method is the same as that of Example 2. The difference is that for those with a component dosage of 0, they are not added during preparation.

[0164] Test Example 2

[0165] Determine the effects of the feeds prepared in Example 2 and Comparative Examples 7-12 on the growth and development of Drosophila melanogaster with vestigial wings. The specific treatment methods for each feed are as follows: Anesthetize the adult Drosophila melanogaster with vestigial wings with ether, select 5 pairs of male and female adults, add them to the Drosophila culture bottle containing the feed, and place 10 pieces of poplar wood filaments with a length of 8-12 cm on the feed for the Drosophila to climb. Observe and record the growth and development of different Drosophila every day, and record the development time from Drosophila eggs to pupae, from pupae to adults, the weight of adults, the number of adults, and the lifespan of adults. Each feed is subjected to 3 parallel repeated experiments, and the results are shown in Tables 7-10.

[0166] Table 7 Effects of Different Feeds on the Growth and Development of Drosophila melanogaster with Vestigial Wings (Replicate 1)

[0167]

[0168] Table 8 Effects of Different Feeds on the Growth and Development of Drosophila melanogaster with Vestigial Wings (Replicate 2)

[0169]

[0170] Table 9 Effects of Different Feeds on the Growth and Development of Drosophila melanogaster with Vestigial Wings (Replicate 3)

[0171]

[0172] Table 10 Effects of Different Feeds on the Growth and Development of Drosophila melanogaster with Vestigial Wings (Average Value)

[0173]

[0174] As can be seen from Tables 7-10, the feed prepared in Example 2 has the best effect on the growth and development of Drosophila melanogaster with vestigial wings, obtaining the shortest development cycle (13.67 days), increasing the weight of adult Drosophila melanogaster (3.8 mg), having the largest number of adult Drosophila melanogaster with vestigial wings in the offspring (106 individuals), and the longest average lifespan of adults (57 days).

[0175] Application Example 1

[0176] A method for raising Rhinocoris fuscipes with the device 1 of the present invention and Drosophila melanogaster with vestigial wings consists of the following steps:

[0177] 1) Cultivation of Drosophila melanogaster with vestigial wings:

[0178] The primary feed prepared in step 2) of Example 1 was dispensed into the feed chamber 11 of the feed box 10 (25 ml disposable sauce cup), and then the remaining 20 wt.% of yeast powder was evenly distributed into the feed chamber 11 and sprinkled on the surface of the primary feed. After the primary feed was completely cooled, the feed box 10 containing Feed A was obtained.

[0179] The feed box 10 containing Feed A was installed on the connecting plate 92. 20 poplar wood filaments with a length of 15 - 20 cm and a thickness of 0.2 - 0.4 mm were added to the bottom of the wingless fruit fly breeding chamber 21, and then 60 adult wingless fruit flies were poured into each wingless fruit fly breeding chamber 21, and the cover body 70 was covered.

[0180] After the adult wingless fruit flies mated and laid eggs in the wingless fruit fly breeding chamber 21 for 5 days, before the fruit fly larvae climbed out of the feed box 10, the cover body 70 was opened, and the feed box 10 was removed from the connecting plate 92. A new feed box 10 containing Feed A was reinstalled on the connecting plate 92 for the adult wingless fruit flies to continue laying eggs.

[0181] 20 poplar wood filaments with a length of 15 - 20 cm and a thickness of 0.2 - 0.4 mm were added to the bottom of the culture chamber 31. The removed feed box 10 containing fruit fly larvae was installed on a new connecting plate 92. The new connecting plate 92 and the new cover body 70 were connected by a connecting rod 91, and the new cover body 70 was connected to the wingless fruit fly breeding box. The feed box 10 containing fruit fly larvae was placed in the culture chamber 31. The escape hole 32 on the cover body 70 was plugged with absorbent cotton to prevent fruit flies from escaping. Finally, the wingless fruit fly culture box 30 was placed in a climate incubator at a temperature of 25 ± 1°C and a humidity of 70% ± 10%. After the eggs of the wingless fruit flies hatched and the larvae climbed out and pupated on the inner wall of the culture chamber 31, it was ready for use.

[0182] 2) Cultivation of wingless large fruit flies:

[0183] The primary feed prepared in step 2) of Example 2 was dispensed into the feed chamber 11 of the feed box 10 (25 ml disposable sauce cup), and then the remaining 20 wt.% of yeast powder was evenly distributed into the feed chamber 11 and sprinkled on the surface of the primary feed. After the primary feed was completely cooled, the feed box 10 containing Feed B was obtained.

[0184] The feed box 10 containing Feed A was installed on the connecting plate 92. 20 poplar wood filaments with a length of 15 - 20 cm and a thickness of 0.2 - 0.4 mm were added to the bottom of the wingless fruit fly breeding chamber 21, and then 60 adult wingless large fruit flies were poured into each wingless fruit fly breeding chamber 21, and the cover body 70 was covered.

[0185] After the adult Drosophila melanogaster with vestigial wings mates and lays eggs in the vestigial-wing Drosophila breeding cavity 21 for 7 days, and before the Drosophila larvae climb out of the feed box 10, open the cover body 70, remove the feed box 10 from the connection plate 92, reinstall a feed box 10 containing new feed B on the connection plate 92 for the adult Drosophila with vestigial wings to continue laying eggs.

[0186] Add 20 poplar wood filaments with a length of 15 - 20 cm and a thickness of 0.2 - 0.4 mm at the bottom of the culture cavity 31. Install the removed feed box 10 containing Drosophila larvae on a new connection plate 92. The new connection plate 92 and the new cover body 70 are connected by a connecting rod 91, and the new cover body 70 is connected to the vestigial-wing Drosophila breeding box. Place the feed box 10 containing Drosophila larvae in the culture cavity 31. The escape hole 32 on the cover body 70 is plugged with absorbent cotton to prevent Drosophila from escaping. Finally, place the vestigial-wing Drosophila culture box 30 in a climate incubator at a temperature of 25 ± 1°C and a humidity of 70% ± 10%. Wait for the eggs of the adult Drosophila melanogaster with vestigial wings to hatch. After the larvae climb out and pupate on the inner wall of the culture cavity 31, it is ready for use.

[0187] 3) Rearing of Rhynocoris fuscipes:

[0188] Collect the vestigial-wing Drosophila culture boxes that have pupated on the inner wall of the culture cavity 31 in steps 1) and 2). When the pupae on the inner wall of the culture cavity 31 start to emerge as adults and the number of adults in each box reaches more than 30, remove the absorbent cotton on the escape hole 32 of the cover body 70, connect the escape hole 32 to the Rhynocoris fuscipes rearing box 40 through a connecting tube 50. Utilize the upward climbing tendency of the adult vestigial-wing Drosophila. The adult vestigial-wing Drosophila in the culture cavity 31 climbs from the escape hole 32 to the connecting tube 50 and then drops into the rearing cavity 41.

[0189] According to the predation ability of different instars of Rhynocoris fuscipes, the 1 - 3 instar nymphs of Rhynocoris fuscipes are fed with adult vestigial-wing Drosophila melanogaster, and the nymphs and adults of Rhynocoris fuscipes older than 3 instars are fed with adult vestigial-wing Drosophila melanogaster. In this way, the artificial rearing of the entire generation of Rhynocoris fuscipes can be completed.

[0190] Comparative Application Example 1

[0191] A method similar to Application Example 1, the difference is that step 2) is not carried out, and in step 3), the nymphs and adults of Rhynocoris fuscipes older than 3 instars are also fed with adult vestigial-wing Drosophila melanogaster.

[0192] Comparative Application Example 2

[0193] A method similar to Application Example 1, the difference is that step 1) is not carried out, and in step 3), the 1 - 3 instar nymphs of Rhynocoris fuscipes are also fed with adult vestigial-wing Drosophila melanogaster.

[0194] Comparative Application Example 3

[0195] A method similar to Application Example 1, except that in steps 1) and 2), incubation treatment is not carried out, and step 3) is as follows: For the nymphs of Rhynocoris fuscipes aged 1 to 3, they are directly fed with the eggs of Drosophila melanogaster with broken wings, and for the nymphs and adults of Rhynocoris fuscipes older than 3 years old, they are directly fed with the eggs of Drosophila melanogaster with large broken wings.

[0196] Comparative Application Example 4

[0197] Feeding Rhynocoris fuscipes with Myzus persicae, the specific method is as follows: First, breed tobacco seedlings in flowerpots, and then breed Myzus persicae on the tobacco seedlings. The newly hatched nymphs to the 3rd instar nymphs of Rhynocoris fuscipes are fed with Myzus persicae aged 1 to 2, and the 4th instar nymphs, 5th instar nymphs and adults of Rhynocoris fuscipes are fed with wingless and winged Myzus persicae aged 3 to 4.

[0198] Comparative Application Example 5

[0199] Feeding Rhynocoris fuscipes with Spodoptera litura, the specific method is as follows: First, breed Spodoptera litura with feed, and then feed the newly hatched nymphs of Rhynocoris fuscipes with the 1st instar larvae of Spodoptera litura, feed the 2nd to 3rd instar nymphs of Rhynocoris fuscipes with the 2nd instar larvae of Spodoptera litura, and feed the 4th instar nymphs, 5th instar nymphs and adults of Rhynocoris fuscipes with the 3rd instar larvae of Spodoptera litura.

[0200] Comparative Application Example 6

[0201] Feeding Rhynocoris fuscipes with Tenebrio molitor, the specific method is as follows: First, purchase Tenebrio molitor egg masses from the market, and after hatching, feed them with wheat bran and vegetable leaves. Then, feed the newly hatched nymphs of Rhynocoris fuscipes with the 1st instar larvae of Tenebrio molitor, feed the 2nd to 3rd instar nymphs of Rhynocoris fuscipes with the 2nd instar larvae of Tenebrio molitor, and feed the 4th instar nymphs, 5th instar nymphs and adults of Rhynocoris fuscipes with the 3rd to 4th instar larvae of Tenebrio molitor.

[0202] Comparative Application Example 7

[0203] Feeding Rhynocoris fuscipes with Galleria mellonella, the specific method is as follows: First, feed Galleria mellonella with feed, and then feed the newly hatched nymphs of Rhynocoris fuscipes with the 1st instar larvae of Galleria mellonella, feed the 2nd to 3rd instar nymphs of Rhynocoris fuscipes with the 2nd instar larvae of Galleria mellonella, and feed the 4th instar nymphs, 5th instar nymphs and adults of Rhynocoris fuscipes with the 3rd to 4th instar larvae of Galleria mellonella.

[0204] Test Example 3

[0205] Determine the effects of different methods of raising Rhynocoris fuscipes in Application Example 1 and Comparative Application Examples 1 to 7. The specific determination method is as follows:

[0206] Respectively determine and record the survival rates of different instars of Rhynocoris fuscipes, the pre-oviposition period, oviposition period, number of ovipositions, egg production amount, egg development duration and hatching rate of female adults under different methods.

[0207] The determination results are shown in Tables 11 to 13.

[0208] Table 11 Effects of different methods on the survival rates of nymphs and adults of *Pristhesancus plagipennis*

[0209]

[0210] Table 12 Effects of different methods on the pre-oviposition period and oviposition period of *Pristhesancus plagipennis*

[0211] Group Pre-oviposition period / d Oviposition period / d Number of ovipositions Number of eggs laid per single oviposition / grain Application Example 1 6.8±0.52a 42.2±1.93a 6.2±0.23a 22.5±0.82a Comparative Application Example 1 7.7±0.89b 32.5±2.13b 2.5±0.23c 10.3±0.74d Comparative Application Example 2 7.6±0.75b 33.7±2.08b 2.4±0.25c 11.4±0.79d Comparative Application Example 3 12.4±1.02c 25.1±2.35c 2.2±0.21c 5.3±0.13e Comparative Application Example 4 7.1±0.56a 39.9±2.02a 4.5±0.15b 19.5±1.06b Comparative Application Example 5 7.2±0.63a 39.4±1.34a 5.6±0.15a 18.9±1.11b Comparative Application Example 6 7.6±0.67b 38.3 ± 2.06 ab 3.2±0.13c 15.2±1.09d Comparative Application Example 7 7.8±0.51b 40.1±2.94a 5.8±0.19a 18.5±1.19b

[0212] Table 13 Effects of different methods on the developmental duration and hatching rate of eggs of *Pristhesancus plagipennis*

[0213]

[0214]

[0215] As can be seen from Table 11, the method provided by the present invention has the highest survival rate for rearing *Pristhesancus plagipennis*. The survival rates of the 1st - 5th instar nymphs, male and female adults reach 96.5%, 94.6%, 91.4%, 88.6%, 85.1%, 82.4% and 83.5% respectively. The lowest survival rate is in Comparative Application Example 3, and the result shows that *Pristhesancus plagipennis* does not like to feed on Drosophila eggs.

[0216] As can be seen from Table 12 and Table 13, the method provided by the present invention has the best effect on rearing *Pristhesancus plagipennis*, with the largest number of oviposition times (6.2 times), the largest average number of eggs laid per oviposition (22.5 eggs), and the egg hatching rate reaching 92.45%.

[0217] In summary, based on the predation characteristics of *Pristhesancus plagipennis* in different insect states, the present invention uses adult Drosophila melanogaster with vestigial wings to rear *Pristhesancus plagipennis*, eliminating the cumbersome steps of cultivating host plants to breed prey, reducing the rearing cost, improving the efficiency of rearing *Pristhesancus plagipennis*, and enabling the completion of the entire generation of *Pristhesancus plagipennis* rearing with vestigial-winged Drosophila melanogaster indoors.

[0218] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for rearing Rhynocoris fuscipes using wingless Drosophila melanogaster, comprising: Feeding **Pristhesancus plagipennis** with adult fruit flies having vestigial wings; The adult fruit flies having vestigial wings include adult **Drosophila melanogaster** and adult **Drosophila hydei**. The 1st - 3rd instar nymphs of **Pristhesancus plagipennis** are fed with adult **Drosophila melanogaster**, and the nymphs of **Pristhesancus plagipennis** older than the 3rd instar and / or the adults of **Pristhesancus plagipennis** are fed with adult **Drosophila hydei**; The method for obtaining the adult fruit flies having vestigial wings includes: feeding the adult fruit flies having vestigial wings with feed, the adult fruit flies having vestigial wings mating and laying eggs, and the hatched eggs yielding larvae of fruit flies having vestigial wings; the larvae of fruit flies having vestigial wings pupating after feeding on the feed, and the pupae emerging into adult fruit flies having vestigial wings.

2. The method according to claim 1, wherein The temperature for hatching is 25 ± 1 °C, and the humidity is 70% ± 10%.

3. The method according to claim 1 or 2, characterized in that, The feed includes feed A and / or feed B in individual packages; Feed A includes the following raw materials in parts by mass: 500 parts of water, 100 - 120 parts of corn flour, 100 - 120 parts of potato granules, 10 - 12 parts of cinnamon, 20 - 30 parts of alfalfa meal, 20 parts of dried kelp powder, 20 parts of dried watermelon powder, 20 parts of yeast powder, 8 - 10 parts of rose hips, 8 - 10 parts of hibiscus flowers, 8 - 10 parts of marigold flowers, 3 - 5 parts of red pepper powder, 3 - 5 parts of turmeric root powder, 8 - 10 parts of fructose, 2 parts of compound vitamins, 1 part of methylparaben, 0.5 - 0.8 part of table salt, 0.5 - 0.8 part of citrate, 0.5 - 0.8 part of calcium malate, 0.5 - 0.8 part of zinc methionine, 0.5 - 0.8 part of magnesium glycinate chelate, 0.5 - 0.8 part of copper methionine, and 0.5 - 0.8 part of calcium propionate; The feed B comprises the following raw materials for preparation in parts by mass: 500 parts of water, 100 - 120 parts of corn flour, 100 - 120 parts of flour, 20 - 30 parts of fruit puree, 10 - 15 parts of cinnamon, 10 - 15 parts of alfalfa meal, 10 - 15 parts of rose flowers, 10 - 15 parts of hibiscus flowers, 10 - 15 parts of yeast powder, 8 - 10 parts of fructose, 2 - 3 parts of malic acid, 2 parts of potassium citrate, 1 part of vitamin A supplement, 1 part of vitamin D supplement, 0.5 - 0.8 parts of zinc methionine, 0.5 - 0.8 parts of copper methionine, 1 part of methylparaben, 1 part of potassium sorbate, 0.8 - 1.0 parts of choline chloride, 0.8 - 1.0 parts of pantothenic acid, 0.5 - 0.8 parts of β - carotene, 0.5 part of pyridoxine hydrochloride, 0.5 - 1.0 parts of folic acid and 0.5 part of vitamin B 12 0.5 part.

4. The method according to claim 1 or 2, characterized in that, The device for feeding **Pristhesancus plagipennis** includes: At least one feed box (10) having a feed cavity (11) for containing feed; A breeding box (20) for fruit flies having vestigial wings having a breeding cavity (21), the feed box (10) and the breeding box (20) for fruit flies having vestigial wings being detachably connected; a plurality of adult fruit flies having vestigial wings are placed in the breeding cavity (21), and the plurality of adult fruit flies having vestigial wings can lay eggs in the feed cavity (11); A culture box (30) for fruit flies having vestigial wings having a culture cavity (31), the feed box (10) in the breeding cavity (21) and the culture box (30) for fruit flies having vestigial wings being detachably connected; an escape hole (32) communicating the culture cavity (31) with the outside is provided on the culture box (30) for fruit flies having vestigial wings, and a plugging member for closing the culture cavity (31) and the outside is provided at the escape hole (32), and the plugging member is detachably connected to the culture box (30) for fruit flies having vestigial wings; Red-colored Rui hunting bug breeding box (40), having a breeding cavity (41) for breeding red-colored Rui hunting bugs, an access hole (42) is provided on the red-colored Rui hunting bug breeding box (40) which can communicate the breeding cavity (41) with the outside; and a connecting pipe (50), both ends of the connecting pipe (50) are respectively detachably connected and communicated with the escape hole (32) and the access hole (42), and the adults cultured in the wingless fruit fly culture box (30) can enter the red-colored Rui hunting bug breeding box (40) through the escape hole (32), the connecting pipe (50) and the access hole (42).

5. The method according to claim 4, wherein Ventilation holes (60) are provided on both the wingless fruit fly breeding box (20), the wingless fruit fly culture box (30) and the red-colored Rui hunting bug breeding box (40); and the escape hole (32) is provided at the top of the wingless fruit fly culture box (30).

6. The method according to claim 4, characterized in that, The number of the wingless fruit fly breeding boxes (20) and the wingless fruit fly culture boxes (30) are both set to be at least two; the two wingless fruit fly breeding boxes (20) are respectively a first breeding box and a second breeding box, and the two wingless fruit fly culture boxes (30) are respectively a first culture box and a second culture box; The first breeding box and the second breeding box are respectively used to place adult Drosophila melanogaster and adult Drosophila hydei. The feed box (10) in the first breeding box is used to contain Drosophila melanogaster feed, and the feed box (10) in the second breeding box is used to contain Drosophila hydei feed; The first culture box is used to place the feed box (10) in the first breeding box and cultivate adult Drosophila melanogaster; The second culture box places the feed box (10) in the second breeding box and cultivates adult Drosophila hydei; The red-colored Rui hunting bug breeding box (40) is communicated with the first culture box or the second culture box through the connecting pipe (50).

7. The method according to claim 4, wherein Fixing components (90) are fixedly arranged in both the wingless fruit fly breeding box (20) and the wingless fruit fly culture box (30). The upper end of the feed box (10) is open, and the fixing component (90) is used for detachably connecting with the feed box (10).

8. The method according to claim 7, wherein Each fixing component (90) includes a connecting rod (91) and a connecting disk (92). The feed box (10) is arranged in a cylindrical shape. One end of the connecting rod (91) is fixedly connected with the top inside the wingless fruit fly breeding box (20) or the wingless fruit fly culture box (30), and the other end is fixedly connected with the connecting disk (92). The connecting disk (92) is detachably connected with the upper end of the feed box (10), and an insect passing hole (93) is provided on the connecting disk (92) which can communicate the feed cavity (11) with the breeding cavity (21) or the culture cavity (31) and is used for allowing wingless fruit flies to pass through.

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