A method for cultivating Paphiopedilum floridulus

Abstract

The present invention discloses a method for cultivating a florid Paphiopedilum callosum. The method comprises the following steps: selecting plants with 5-6 leaves, strong growth, and a well-developed root system for root irrigation with a 6-BA solution at a concentration of 200 to 800 mg / L, treating them once every two weeks for a total of 5-6 times, and performing unique fertilizer and water management during the subsequent cultivation process. The present invention utilizes a 6-BA solution to root-irrigate seedlings transplanted for one year to significantly increase the number of tillering buds, and performs appropriate fertilizer and water management during the subsequent cultivation process, which can cause most of the tillering lateral buds and main buds to bloom together, significantly increasing the number of flowers. The method can promote a single Paphiopedilum callosum plant to bloom multiple branches, improve its ornamental value, fill domestic and international gaps, and have a positive driving effect on promoting the development of my country's Paphiopedilum industry.

Description

Technical Field

[0001] The invention belongs to the technical field of plant cultivation, and particularly relates to a cultivation method of Paphiopedilum floridulus. Background Art

[0002] Paphiopedilum callosum, native to Vietnam, Thailand, and other Southeast Asian regions, is one of the most widely cultivated and highly ornamental native species of the genus Paphiopedilum. It is also an important parent of the "Magic Emperor" family of Paphiopedilum cultivars. Under conventional natural cultivation conditions, Paphiopedilum callosum test tube seedlings typically do not tiller before flowering. Individual seedlings often have one inflorescence with two flower buds, but in most cases only produce a single flower. Flowering plants typically produce only a single lateral bud, followed by another flower in the second year, which is usually a single flower. Although our laboratory's patented technology, using gibberellin (GA3) injection treatment, can significantly increase the double-flowering rate of individual flowers and advance the flowering period, it does not increase the number of tillers or flowering branches (A Method for Improving the Double-Flowering Rate and Regulating the Flowering Period of Paphiopedilum Callosum. Patent Authorization Number: ZL202110378693.8).

[0003] According to literature search, there are no reports at home and abroad on the use of cytokinin treatment to promote tillering and flowering of Paphiopedilum. Summary of the Invention

[0004] The present invention aims to provide a method for cultivating Paphiopedilum floridianum. The method utilizes a 6-benzylaminopurine (6-BA) solution as a root irrigation treatment for one-year-old transplanted seedlings to significantly increase the number of tillering buds. Proper fertilizer and water management during subsequent cultivation can cause most of the tillering lateral buds to bloom together with the main bud, significantly increasing the number of flowers.

[0005] The present invention provides a cultivation method for Paphiopedilum floridulus, which specifically comprises the following steps: selecting plants with 5-6 leaves, strong growth and developed root systems, performing root irrigation treatment with 6-benzylaminopurine solution, and performing unique fertilizer and water management in the subsequent cultivation process.

[0006] Preferably, the concentration of the 6-benzylaminopurine solution is 200-800 mg / L.

[0007] More preferably, the concentration of the 6-benzylaminopurine solution is 400 mg / L.

[0008] Preferably, the root irrigation treatment is performed once every two weeks, for a total of 5-6 times.

[0009] Preferably, the fertilizer and water management includes the step of implementing high-phosphorus fertilizer and water management to promote flowering after root irrigation with 6-benzylaminopurine solution for 6 months.

[0010] Preferably, the fertilizer and water management is specifically as follows: spraying 2000-3000 times the water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer once a week, and applying 10-20 grains of Haokangduo 180-day slow-release fertilizer per pot on the surface of the pot; after 6 months, spraying 2000-3000 times the water-soluble Huaduoduo No. 215 high-phosphorus fertilizer once a week, and applying 10-20 grains of Aolv A2 180-day slow-release fertilizer per pot on the surface of the pot.

[0011] More preferably, the fertilizer and water management is specifically as follows: spray 3000 times the water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer once a week, and apply 20 grains of Haokangduo 180-day slow-release fertilizer per pot on the surface of the pot; after 6 months, spray 2000 times the water-soluble Huaduoduo No. 215 high-phosphorus fertilizer once a week, and apply 20 grains of Aolv A2 180-day slow-release fertilizer per pot on the surface of the pot.

[0012] Preferably, the early cultivation and management steps of Paphiopedilum callosum plants are:

[0013] Sterile, 5-8 cm tall Paphiopedilum callosum seedlings are sown in test tubes in September or October, planted in 1.7-inch white plastic cups using imported Israeli sphagnum moss. For planting, the test tube seedling bottles are placed in a greenhouse for 1-2 weeks to harden. The rooted seedlings are then removed from the bottles, the attached culture medium cleaned, and the roots of the seedlings are wrapped with sphagnum moss that has been soaked in water for at least 24 hours and squeezed dry. The seedlings are then planted in 1.7-inch pots in a greenhouse, maintained at 10-32°C and 70-90% humidity. Maintain ventilation throughout the greenhouse, and after one month, the survival rate should reach over 95%. Spray the water-soluble balanced fertilizer, Huaduoduo No. 1 General Purpose Fertilizer (nitrogen, phosphorus, and potassium at a ratio of 20-20-20) at a 3000-4000 times dilution once a week.

[0014] After 6-7 months, from March to May of the following year, repot the plants in 2.5-inch white plastic cups using a 2:1 mixture of plant stone and pine bark. For one month after repotting, spray the plants weekly with a 2000-3000x diluted water-soluble balanced fertilizer, Huaduoduo No. 1 (nitrogen, phosphorus, and potassium in a 20-20-20 ratio). In September or October of the following year, one year after the seedlings were transplanted, the plants were cultivated.

[0015] The present invention provides a method for cultivating Paphiopedilum callosum, which can promote the multi-flowering of Paphiopedilum callosum and the production of multiple flowers per plant, thereby improving its ornamental value. This method fills a gap in domestic and international research and has a positive role in promoting the development of the Paphiopedilum industry in my country. The present invention is technically feasible and has high application value. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1This shows the tillering situation of Paphiopedilum callosum after root irrigation with 6-BA solution at a concentration of 400 mg / L.

[0017] Figure 2 The results show that after the roots of Paphiopedilum callosum were irrigated with 400 mg / L 6-BA solution, 3000 times diluted water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer was sprayed once a week, and 20 grains of Haokangduo slow-release fertilizer were applied to each pot for one year.

[0018] Figure 3 After the callus orchid was root-irrigated with 400 mg / L 6-BA solution, it was sprayed with 3000 times diluted water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer once a week, and 20 grains of Haokangduo slow-release fertilizer were applied on the pot surface; 6 months later, it was sprayed with 2000 times diluted water-soluble Huaduoduo No. 215 high-phosphorus fertilizer once a week, and 20 grains of Aolv A2 slow-release fertilizer were applied on the pot surface to observe its flowering. DETAILED DESCRIPTION

[0019] The following examples are provided to further illustrate the present invention, but are not intended to limit the present invention.

[0020] Example 1

[0021] 1. Early cultivation and management of Paphiopedilum callosum

[0022] Sterile, 7-cm-tall Paphiopedilum callosum seedlings were sown in September using imported Israeli sphagnum moss in 1.7-inch white plastic cups. After acclimating the seedlings in the jars for two weeks, the rooted seedlings were removed from the jars, the attached culture medium cleaned, and the roots of the seedlings were wrapped with sphagnum moss that had been soaked in water for at least 24 hours and squeezed dry. The seedlings were then planted in 1.7-inch pots in a greenhouse, maintained at 32°C and 70% humidity. Maintaining ventilation was crucial. After one month, the survival rate reached 97%. The seedlings were then sprayed weekly with a 3500-fold dilution of the water-soluble balanced fertilizer, Huaduoduo No. 1 (nitrogen, phosphorus, and potassium ratio: 20-20-20).

[0023] Seven months later, in May of the following year, repot the plant in a 2.5-inch white plastic cup using a 2:1 mixture of phytoliths and pine bark. For one month after repotting, spray the plant with a 2500-fold dilution of Huaduoduo No. 1 universal fertilizer (nitrogen, phosphorus, and potassium in a ratio of 20-20-20) weekly.

[0024] 2. Effects of different 6-BA treatments and fertilization methods on the flowering of Paphiopedilum callosum

[0025] 2.1 Treatment of Paphiopedilum callosum with foliar spraying or root irrigation of 6-BA solution of varying concentrations: In September of the following year, one year after the test tube seedlings were transplanted, select plants with 5-6 leaves, strong growth, and well-developed root systems for foliar spraying or root irrigation of 6-BA solution of varying concentrations. The concentrations of 6-BA solution were 200mg / L, 400mg / L, 600mg / L, and 800mg / L, respectively, with treatments every two weeks for a total of six treatments. One week after treatment, spray the water-soluble balanced fertilizer Huaduoduo No. 1 general-purpose fertilizer (nitrogen, phosphorus, and potassium are 20-20-20) at a dilution of 3000 times once a week, and apply 20 capsules of Haokangduo (nitrogen, phosphorus, and potassium are 14-12-14) to each pot surface, which is a slow-release fertilizer with a 180-day fertilizer retention period. After six months of treatment, the plants were sprayed weekly with a 2000-fold dilution of the water-soluble Huaduoduo 215 high-phosphate fertilizer (nitrogen, phosphorus, and potassium ratios of 10-52-10). Twenty capsules of Aolv A2 (nitrogen, phosphorus, and potassium ratios of 9-14-19) were applied to each pot surface, along with a 180-day slow-release fertilizer. After another six months of this management, the effects of different 6-BA treatments on the tillering and flowering of Paphiopedilum callosum were analyzed. A control group was also established, without 6-BA treatment but receiving the same fertilizer and water management. The results are shown in Table 1.

[0026] Table 1. Effects of different 6-BA treatments on tillering and flowering of Paphiopedilum callosum

[0027]

[0028]

[0029] Note: Treatment method: once every two weeks

[0030] Number of buds per plant = total number of buds per plant per treatment / number of mother plants treated

[0031] Number of flowering branches = number of flowering branches in each treatment group / total number of pots

[0032] Flowering rate = number of flowering buds per treatment / total number of buds

[0033] As shown in Table 1, root irrigation with 6-BA solution promoted tillering and flowering of Paphiopedilum callose better than foliar spraying, and the root irrigation with 400 mg / L 6-BA solution had the best effect.

[0034] 2.2 Differentiated Fertilizer and Water Management for Paphiopedilum Callosum: In September of the following year, one year after the test tube seedlings were transplanted, select plants with 5-6 leaves, strong growth, and a well-developed root system and apply a root irrigation treatment of 400mg / L 6-BA solution. Treatments were repeated every two weeks for a total of six times. One week after treatment, the water-soluble balanced fertilizer Huaduoduo No. 1 (nitrogen, phosphorus, and potassium ratios of 20-20-20) was sprayed weekly in various dilutions. Furthermore, Haokangduo (nitrogen, phosphorus, and potassium ratios of 14-12-14) slow-release fertilizer with a 180-day retention period was applied to each pot surface in varying amounts. About six months after treatment, 3.33 tiller buds grew at the base of the plant. This treated material was sprayed weekly with water-soluble Huaduoduo 215 high-phosphorus fertilizer (nitrogen, phosphorus, and potassium ratios of 10-52-10) at varying dilutions. Aolu A2 (nitrogen, phosphorus, and potassium ratios of 9-14-19) with a 180-day retention period was also applied to each pot surface in varying amounts of slow-release fertilizer. After another six months of this management, the effects of different fertilization methods on the flowering of Paphiopedilum callosum were statistically analyzed. The results are shown in Table 2.

[0035] Table 2 Effects of different fertilization methods on the flowering of Paphiopedilum callosum

[0036]

[0037]

[0038] Note: The tillering buds obtained after 6 months of root irrigation with 400 mg / L 6-BA solution were used as materials for flowering cultivation experiments.

[0039] As can be seen from Table 2, after the calliphizus orchid was root-irrigated with a 6-BA solution at a concentration of 400 mg / L, it was sprayed with 3000 times the water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer once a week, and 20 grains of Haokangduo slow-release fertilizer were applied to each pot on the surface of the pot; 6 months later, it was sprayed with 2000 times the water-soluble Huaduoduo No. 215 high-phosphorus fertilizer once a week, and 20 grains of Aolv A2 slow-release fertilizer were applied to each pot on the surface of the pot, which had the best effect on promoting the flowering of the calliphizus orchid.

[0040] Example 2

[0041] 1. The early stage cultivation and management of Paphiopedilum callosum are the same as those in Example 1.

[0042] 2. Carry out root irrigation and fertilization treatment on Paphiopedilum callosum with 6-BA solution: In September of the following year, one year after the test tube seedlings were transplanted, select plants with 5-6 leaves, strong growth and well-developed root systems for root irrigation treatment with 6-BA solution at a concentration of 400 mg / L. Treat once every two weeks, for a total of 6 times. One week after treatment, spray 3000 times the water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer (nitrogen, phosphorus and potassium are 20-20-20) once a week, and apply 20 grains of Haokangduo (nitrogen, phosphorus and potassium are 14-12-14) slow-release fertilizer for 180 days on each pot surface. About 6 months after treatment, 3.33 tiller buds will grow at the base of the plant ( Figure 1 ), on the treated materials, that is, after 6 months of treatment, spray 2000 times liquid of water-soluble Huaduoduo No. 215 high phosphorus fertilizer (nitrogen, phosphorus, and potassium are 10-52-10) once a week, and apply 20 grains of Aolv A2 (nitrogen, phosphorus, and potassium are 9-14-19) slow-release fertilizer with a fertilizer retention period of 180 days on each pot surface. After another 6 months of such management, the plants bloomed in April-May of the following year, and the number of flowers in each pot was 2.80.

[0043] After the roots of Paphiopedilum callosum were irrigated with 400mg / L 6-BA solution, 3000 times diluted water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer was sprayed once a week, and 20 capsules of Haokangduo slow-release fertilizer were applied to each pot surface; after 6 months, 2000 times diluted water-soluble Huaduoduo No. 215 high phosphorus fertilizer was sprayed once a week, and 20 capsules of Aolv A2 slow-release fertilizer were applied to each pot surface. The flowering situation can be seen from the following figure: Figure 3 .

[0044] As a comparison, the present invention also investigated the development of Paphiopedilum callosum after root irrigation with a 6-BA solution at a concentration of 400 mg / L, spraying a water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer 3000 times liquid once a week, and applying 20 capsules of Haokangduo slow-release fertilizer per pot on the pot surface for one year. The results are shown in FIG. Figure 2 .

[0045] Example 3

[0046] 1. The early stage cultivation and management of Paphiopedilum callose are the same as those in Example 1.

[0047] 2. Root irrigation and fertilization of Paphiopedilum callosum with 6-BA solution: In September of the following year, one year after the test tube seedlings were transplanted, select plants with 5-6 leaves, strong growth, and a well-developed root system and root irrigation with 400mg / L of 6-BA solution. Treatments were conducted every two weeks for a total of six times. One week after treatment, spray the plants weekly with a 3000-fold dilution of the water-soluble balanced fertilizer Huaduoduo No. 1 (nitrogen, phosphorus, and potassium ratio of 20-20-20). Apply 10 capsules of Haokangduo (nitrogen, phosphorus, and potassium ratio of 14-12-14) to each pot surface, which provides a 180-day fertilizing effect. About 6 months after the treatment, 3.33 tiller buds will grow at the base of the plant. On the treated material, that is, 6 months after the treatment, spray 2000 times of water-soluble Huaduoduo No. 215 high-phosphorus fertilizer (nitrogen, phosphorus, and potassium are 10-52-10) once a week, and apply 15 grains of Aolv A2 (nitrogen, phosphorus, and potassium are 9-14-19) slow-release fertilizer with a 180-day retention period on each pot. After another 6 months of such management, the plants will bloom in April-May of the following year, with 2.60 flowers per pot.

[0048] Example 4

[0049] 1. The early stage cultivation and management of Paphiopedilum callose are the same as those in Example 1.

[0050] 2. Root irrigation and fertilization of Paphiopedilum callosum with 6-BA solution: In September of the following year, one year after the test tube seedlings were transplanted, select plants with 5-6 leaves, strong growth, and a well-developed root system and root irrigation with 400mg / L of 6-BA solution. Treatments were conducted every two weeks for a total of six times. One week after treatment, spray the plants weekly with a 2500-fold dilution of the water-soluble balanced fertilizer Huaduoduo No. 1 (nitrogen, phosphorus, and potassium ratio of 20-20-20). Apply 10 capsules of Haokangduo (nitrogen, phosphorus, and potassium ratio of 14-12-14) to each pot surface, which provides a 180-day fertilizing effect. About 6 months after the treatment, 3.33 tiller buds will grow at the base of the plant. On the treated material, that is, 6 months after the treatment, spray 2500 times the water-soluble Huaduoduo No. 215 high-phosphorus fertilizer (nitrogen, phosphorus, and potassium are 10-52-10) once a week, and apply 20 grains of Aolv A2 (nitrogen, phosphorus, and potassium are 9-14-19) slow-release fertilizer with a 180-day retention period on each pot. After another 6 months of such management, the plants will bloom in April-May of the following year, and the number of flowers in each pot is 2.853.

[0051] The above are merely preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be construed as limiting the present invention, and the scope of protection of the present invention should be determined by the scope defined in the claims. Persons skilled in the art will appreciate that improvements and modifications may be made without departing from the spirit and scope of the present invention, and such improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for cultivating Paphiopedilum floridulus, characterized in that: The following steps are involved: Select plants with 5-6 leaves, strong growth and developed root system for root irrigation treatment with 6-benzylaminopurine solution. The concentration of 6-benzylaminopurine solution is 400 mg / L. Treat once every two weeks, for a total of 5-6 times. One week after treatment, spray 3000 times diluted water-soluble balanced fertilizer Huaduoduo No. 1 general fertilizer once a week, and apply 20 grains of Haokangduo 180-day slow-release fertilizer on the surface of the pot; after 6 months, spray 2000 times diluted water-soluble Huaduoduo No. 215 high-phosphorus fertilizer once a week, and apply 20 grains of Aolv A2 180-day slow-release fertilizer on the surface of the pot.

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